CN207908243U - A kind of separation and Extraction kit of excretion body - Google Patents
A kind of separation and Extraction kit of excretion body Download PDFInfo
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- CN207908243U CN207908243U CN201820146576.2U CN201820146576U CN207908243U CN 207908243 U CN207908243 U CN 207908243U CN 201820146576 U CN201820146576 U CN 201820146576U CN 207908243 U CN207908243 U CN 207908243U
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Abstract
The utility model provides a kind of separation and Extraction kit of excretion body, includes the waste collection pipe of first level filtering device, the two-stage filter and undertaking two-stage filter of accepting first level filtering device, and application method includes：Sample is transferred to first level filtering device and tentatively filters some the larger impurity removed in sample, then some smaller impurity and waste liquid in removal sample are filtered by two-stage filter, retention obtains being located at the interior suspension for being rich in excretion body of secondary filtration tube body.The utility model is of low cost, simple and efficient to handle, and is not necessarily to specific centrifugation apparatus, has very wide applicable context.
Description
Technical field
The utility model is related to molecular biology and clinical testing techniques fields, and in particular to a kind of separation of excretion body carries
Take kit.
Background technology
Excretion body be by be recessed inwardly by cell endocytic vacuolar membrane formed steep endosomes, steep endosomes again with cell membrane
After fusion, it is discharged into the film property vesica of diameter about 30-150nm in extracellular matrix a kind of.The very big portion of inclusion in excretion body
It is protein to divide, such as：CD9, CD63, CD81, MHC-I etc.；Excessive table can also be detected in the excretion body that tumour cell generates
The protein marker of the specificity reached, such as TRAIL and TGF-β.Also contain in addition to the protein having been found that, in excretion body big
The ingredients such as mRNA, miRNA, cirRNA and DNA fragmentation of amount, since the presence of these contents provides abundant biology letter
Breath, multi-faceted, multi-angle can disclose disease information, this has just contained great potential.Moreover, excretion body derives from a wealth of sources,
Lymphocyte, Dendritic Cells, mast cell and epithelial cell, tumour cell etc. can secrete excretion body, be secreted
Excretion body (Exosome) can also enter amniotic fluid, and ascites, saliva, blood, milk, in urine, this is just greatly expanded detection way
Diameter makes clinical detection become possible to.
Mainly have for the extracting method of excretion body at present：Supercentrifugation, the polymer precipitation method, paramagnetic particle method and ultrafiltration
Method.The shortcomings of these methods there are operating procedures loaded down with trivial details, low separation efficiency, high reagent cost, is unfavorable for excretion body into one
Walk the development of research.
Utility model content
To solve the above problems, the utility model provides a kind of separation and Extraction kit of excretion body, the kit cost
It is cheap, simple and efficient to handle, and it is not necessarily to specific centrifugation apparatus, there is very wide applicable context.
To achieve the above object, the utility model uses following technical scheme：A kind of separation and Extraction kit of excretion body,
Including first level filtering device, accepts the two-stage filter of first level filtering device and accept the waste collection of two-stage filter
Pipe, the first level filtering device include that hollow by-pass filtration tube body, aperture are more than the by-pass filtration film and one of excretion body diameter
Grade infusing device, the by-pass filtration film are located at the bottom in the by-pass filtration tube body, and the level-one infusing device is for producing
Raw thrust makes the liquid in by-pass filtration tube body pass through by-pass filtration film realization by-pass filtration, during the two-stage filter includes
The secondary filtration tube body of sky, aperture are less than the secondary filtration film and two level infusing device of excretion body diameter, the secondary filtration film
Bottom in the secondary filtration tube body, the two level infusing device, which is used to generate thrust, makes liquid in secondary filtration tube body
Body realizes secondary filtration by secondary filtration film.
As a preferred solution, the bottom of the by-pass filtration tube body and the company of matching at the top of secondary filtration tube body
It connects, the pipe diameter of the by-pass filtration tube body is more than the internal diameter of tube body of secondary filtration tube body.
As a preferred solution, the aperture of the by-pass filtration film is 200-1000 nanometers.
As a preferred solution, the level-one infusing device is piston type infusing device.
As a preferred solution, the level-one infusing device include cut somebody's hair and with the push rod cut somebody's hair and be connected,
Described cut somebody's hair matches with the interior wall seamless of the by-pass filtration tube body.
As a preferred solution, the bottom of the secondary filtration tube body matches with waste collection pipe, and described two
The pipe diameter of grade filter body is more than the internal diameter of tube body of waste collection pipe.
As a preferred solution, the aperture of the secondary filtration film is 10-40 nanometers.
As a preferred solution, the two level infusing device is piston type infusing device.
As a preferred solution, the two level infusing device include cut somebody's hair and with the push rod cut somebody's hair and be connected,
Described cut somebody's hair matches with the interior wall seamless of the secondary filtration tube body.
The advantageous effect of the separation and Extraction kit of excretion body provided by the utility model is：
1, isolating and purifying for excretion body is realized in such a way that infusing device generates thrust complement filter film, without specific
Centrifugation apparatus.
2, of low cost, simple and efficient to handle, isolate and purify it is efficient, have very wide applicable context.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of the separation and Extraction kit for excretion body that the utility model embodiment provides；
Fig. 2 is that a kind of separation and Extraction kit for excretion body that the utility model embodiment provides carries out the behaviour of by-pass filtration
Make schematic diagram；
Fig. 3 is that a kind of separation and Extraction kit for excretion body that the utility model embodiment provides carries out the behaviour of secondary filtration
Make schematic diagram.
Specific implementation mode
The utility model is described in further detail with reference to the utility model the drawings and specific embodiments, it should
It points out, for those skilled in the art, without departing from the principle of this utility model, can also do
Go out several improvements and modifications, these improvements and modifications are also considered as the scope of protection of the utility model.
As shown in Figure 1, Figure 2 and Figure 3, the utility model provides a kind of separation and Extraction kit of excretion body, including one
Grade filter device 1, the two-stage filter 2 for accepting first level filtering device 1 and the waste collection pipe for accepting two-stage filter 2
3, the first level filtering device includes that hollow by-pass filtration tube body 11, aperture are more than 12 and of by-pass filtration film of excretion body diameter
Level-one infusing device 13, the by-pass filtration film 12 are located at the bottom in the by-pass filtration tube body 11, and the level-one injects dress
Set 13 makes the liquid in by-pass filtration tube body pass through the realization by-pass filtration of by-pass filtration film 12, the two level mistake for generating thrust
Filter secondary filtration film 22 and two level infusing device that the secondary filtration tube body 21, aperture that device includes hollow are less than excretion body diameter
23, the secondary filtration film 22 is located at the bottom in the secondary filtration tube body 21, and the two level infusing device 23 is for generating
Thrust makes the liquid in secondary filtration tube body pass through the realization secondary filtration of secondary filtration film 12.
The bottom of the by-pass filtration tube body 11 matches connection, the level-one mistake with the top of secondary filtration tube body 21
The pipe diameter of chimney filter body 11 is more than the internal diameter of tube body of secondary filtration tube body 21.
The aperture of the by-pass filtration film 12 is not more than 1 micron.
The level-one infusing device 13 is piston type infusing device.
The level-one infusing device 13 include cut somebody's hair 131, with the push rod 132 cut somebody's hair and be connected, 131 and the institute of cutting somebody's hair
State the 111 seamless matching of inner wall of by-pass filtration tube body 11.
The bottom of the secondary filtration tube body 21 matches with waste collection pipe 3, the pipe of the secondary filtration tube body 21
Body diameter is more than the internal diameter of tube body of waste collection pipe 3.
The aperture of the secondary filtration film 22 is not more than 40 nanometers.
The two level infusing device 23 is piston type infusing device.
The two level infusing device 23 include cut somebody's hair 231, with the push rod 232 cut somebody's hair and be connected, 231 and the institute of cutting somebody's hair
State the 211 seamless matching of inner wall of secondary filtration tube body 21.
A kind of principle of the separation and Extraction kit of excretion body provided by the utility model：Since excretion body is a diameter
The film property vesica of about 30-150nm, therefore Selective Separation can be carried out to sample by the filter membrane combination infusing device of different pore size
To obtain excretion body.
The separation and Extraction kit application method of above-mentioned excretion body is as follows：
1) sample is transferred in hollow by-pass filtration tube body 11, applying to push by level-one infusing device 13 makes sample
By by-pass filtration film 12, some larger impurity in preliminary filtering removal sample, such as most microcapsule bubbles,
2) the secondary filtration tube body 21 of two-stage filter 2 is entered by the sample of 1 filtration treatment of first level filtering device, is led to
It crosses two level infusing device 23 and applies some the smaller impurity for pushing and sample being made to pass through in the filtering removal sample of secondary filtration film 22
And waste liquid, the lower suspension that excretion body is rich in secondary filtration tube body 21 of retention.
3) suspension that will be enriched in excretion body is transferred to preservation pipe, saves backup.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
All any modification, equivalent and improvement etc., should be included in the utility model made by within the spirit and principle of utility model
Protection domain within.
Claims (9)
1. a kind of separation and Extraction kit of excretion body, which is characterized in that including first level filtering device, accept first level filtering device
Two-stage filter and accept the waste collection pipe of two-stage filter, the first level filtering device includes hollow level-one
Filter body, aperture are more than the by-pass filtration film and level-one infusing device of excretion body diameter, and the by-pass filtration film is located at described
Bottom in by-pass filtration tube body, the level-one infusing device make the liquid in by-pass filtration tube body pass through one for generating thrust
Grade filter membrane realizes by-pass filtration, and it is straight that the two-stage filter includes hollow secondary filtration tube body, aperture is less than excretion body
The secondary filtration film and two level infusing device of diameter, the secondary filtration film is located at the bottom in the secondary filtration tube body, described
Two level infusing device makes the liquid in secondary filtration tube body pass through secondary filtration film realization secondary filtration for generating thrust.
2. the separation and Extraction kit of excretion body as described in claim 1, which is characterized in that the by-pass filtration tube body
Match at the top of bottom and secondary filtration tube body, the pipe diameter of the by-pass filtration tube body is more than the pipe of secondary filtration tube body
Internal diameter.
3. the separation and Extraction kit of excretion body as described in claim 1, which is characterized in that the hole of the by-pass filtration film
Diameter is 200-1000 nanometers.
4. the separation and Extraction kit of excretion body as described in claim 1, which is characterized in that the level-one infusing device is
Piston type infusing device.
5. the separation and Extraction kit of excretion body as described in claim 1, which is characterized in that the level-one infusing device packet
Include cut somebody's hair and with the push rod cut somebody's hair and be connected, wherein cut somebody's hair and matched with the interior wall seamless of by-pass filtration tube body, it is described
The pipe diameter of secondary filtration tube body is more than the internal diameter of tube body of waste collection pipe.
6. the separation and Extraction kit of excretion body as described in claim 1, which is characterized in that the bottom of the secondary filtration tube body
Portion matches with waste collection pipe.
7. the separation and Extraction kit of excretion body as described in claim 1, which is characterized in that the aperture of the secondary filtration film
It is 10-40 nanometers.
8. the separation and Extraction kit of excretion body as described in claim 1, which is characterized in that the two level infusing device is
Piston type infusing device.
9. the separation and Extraction kit of excretion body as described in claim 1, which is characterized in that the two level infusing device packet
Include cut somebody's hair and with the push rod cut somebody's hair and be connected, wherein cut somebody's hair and matched with the interior wall seamless of secondary filtration tube body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820146576.2U CN207908243U (en) | 2018-01-29 | 2018-01-29 | A kind of separation and Extraction kit of excretion body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820146576.2U CN207908243U (en) | 2018-01-29 | 2018-01-29 | A kind of separation and Extraction kit of excretion body |
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| CN207908243U true CN207908243U (en) | 2018-09-25 |
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ID=63565366
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| CN201820146576.2U Active CN207908243U (en) | 2018-01-29 | 2018-01-29 | A kind of separation and Extraction kit of excretion body |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110252440A (en) * | 2019-07-25 | 2019-09-20 | 上海市第六人民医院 | A kind of excretion body filtering extraction element and excretion body extracting method |
| CN110426528A (en) * | 2019-07-24 | 2019-11-08 | 云南中烟工业有限责任公司 | A kind of device and its application method for multiple sample pre-treatments |
| CN110820051A (en) * | 2018-12-28 | 2020-02-21 | 广州表观生物科技有限公司 | High-sensitivity fusion gene detection method and application thereof |
-
2018
- 2018-01-29 CN CN201820146576.2U patent/CN207908243U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110820051A (en) * | 2018-12-28 | 2020-02-21 | 广州表观生物科技有限公司 | High-sensitivity fusion gene detection method and application thereof |
| CN110820051B (en) * | 2018-12-28 | 2023-04-28 | 广州表观生物科技有限公司 | High-sensitivity fusion gene detection method and application thereof |
| CN110426528A (en) * | 2019-07-24 | 2019-11-08 | 云南中烟工业有限责任公司 | A kind of device and its application method for multiple sample pre-treatments |
| CN110252440A (en) * | 2019-07-25 | 2019-09-20 | 上海市第六人民医院 | A kind of excretion body filtering extraction element and excretion body extracting method |
| CN110252440B (en) * | 2019-07-25 | 2024-01-23 | 上海市第六人民医院 | Exosome filtering and extracting device and exosome extracting method |
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