CN106085832A - A kind of circulating tumor cell automatic separation system - Google Patents
A kind of circulating tumor cell automatic separation system Download PDFInfo
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- CN106085832A CN106085832A CN201610613548.2A CN201610613548A CN106085832A CN 106085832 A CN106085832 A CN 106085832A CN 201610613548 A CN201610613548 A CN 201610613548A CN 106085832 A CN106085832 A CN 106085832A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M41/48—Automatic or computerized control
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Abstract
The present invention relates to a kind of circulating tumor cell automatic separation system, described erythrocyte cracks addition erythrocyte in unit and cracks liquid and blood, erythrocyte cracks liquid and is cracked by the erythrocyte in blood, described circulating tumor cell separative element includes circulating line, cell separation module, circulation control module, described circulation control module is arranged on described circulating line, described cell separation module is arranged on described circulating line, described circulation control module controls blood and circulates in described circulating line, described cell separation module is by the leukoreduction filter of blood in described circulating line, the circulating tumor cell that described circulating line is flowed out by described circulating tumor cell collector unit is collected.One circulating tumor cell automatic separation system of the present invention, implements the process that the integration to circulating tumor cell is automatically separated, and improves the separation efficiency of circulating tumor cell, and can keep the intact of cell well in separation process, is conveniently analyzed.
Description
Technical field
The present invention relates to a kind of cell automatic separation system, particularly relate to a kind of circulating tumor cell automatic separation system.
Background technology
Along with the development of microflow control technique, research field increases, with the always research of micro-fluidic screening circulating tumor cell
Focus.Circulating tumor cell typically to carry out three steps, and the first step is to remove erythrocyte；Second step is to filter out circulation
Tumor cell；3rd step is whether the cell that evaluation and screening goes out can confirm that as cancerous cell, the at present enforcement to these three steps,
Automaticity is relatively low, and needs to have separated these three step, lacks automatic integratedization and completes the system of these steps
Device, makes the automatic degree of separation process the highest, inefficiency.Further, since implement step by step, blood and cell may be made in reality
It is damaged during executing.
Summary of the invention
Present invention solves the technical problem that and be: build a kind of circulating tumor cell automatic separation system, overcome prior art
In circulating tumor cell separation process, automatic degree is the highest, the technical problem of inefficiency.
The technical scheme is that a kind of circulating tumor cell automatic separation system of structure, crack list including erythrocyte
Unit, circulating tumor cell separative element, circulating tumor cell collector unit, described erythrocyte cracks unit, described circulating tumor
Cell separation unit and described circulating tumor cell collector unit are sequentially connected with, and described erythrocyte cracks addition erythrocyte in unit
Cracking liquid and blood, erythrocyte cracks liquid and is cracked by the erythrocyte in blood, and described circulating tumor cell separative element includes ring
Flow tube road, cell separation module, circulation control module, described circulation control module is arranged on described circulating line, described carefully
Born of the same parents' separation module is arranged on described circulating line, and described circulation control module controls blood recycle stream in described circulating line
Dynamic, described cell separation module is by the leukoreduction filter of blood in described circulating line, described circulating tumor cell collector unit
The circulating tumor cell flowed out by described circulating line is collected.
The further technical scheme of the present invention is: described cell separation module includes leukoreduction filter net.
The further technical scheme of the present invention is: described circulating line is circulating line.
The further technical scheme of the present invention is: described erythrocyte cracks unit and described circulating tumor cell separative element
Between the first shutoff valve is set.
The further technical scheme of the present invention is: described circulating tumor cell separative element and described circulating tumor cell are received
Between collection unit, the second shutoff valve is set.
The further technical scheme of the present invention is: also include that reflux line, described cell separation module include also including receiving
The container of collection tissue fluid and tissue fluid drainage screen, described container is connected by described tissue fluid drainage screen and described reflux line
Described circulating line.
The further technical scheme of the present invention is: described circulation control module is blood pump.
The further technical scheme of the present invention is: described circulating tumor cell collector unit includes that negative pressure cell collects mould
Block.
The further technical scheme of the present invention is: described circulation control module connects described erythrocyte and cracks unit and described
Circulating line.
The further technical scheme of the present invention is: also include that tumor cell marking fluid adds entrance.
The solution have the advantages that: build a kind of circulating tumor cell automatic separation system, crack list including erythrocyte
Unit, circulating tumor cell separative element, circulating tumor cell collector unit, described erythrocyte cracks unit, described circulating tumor
Cell separation unit and described circulating tumor cell collector unit are sequentially connected with, and described erythrocyte cracks addition erythrocyte in unit
Cracking liquid and blood, erythrocyte cracks liquid and is cracked by the erythrocyte in blood, and described circulating tumor cell separative element includes ring
Flow tube road, cell separation module, circulation control module, described circulation control module is arranged on described circulating line, described carefully
Born of the same parents' separation module is arranged on described circulating line, and described circulation control module controls blood recycle stream in described circulating line
Dynamic, described cell separation module is by the leukoreduction filter of blood in described circulating line, described circulating tumor cell collector unit
The circulating tumor cell flowed out by described circulating line is collected.One circulating tumor cell automatic separation system of the present invention, implements
The process being automatically separated the integration of circulating tumor cell, improves the separation efficiency of circulating tumor cell, and point
The intact of cell can be kept well from process, conveniently be analyzed.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention.
Fig. 2 is the structural representation of circulating tumor cell separative element of the present invention.
Detailed description of the invention
Below in conjunction with specific embodiment, technical solution of the present invention is further illustrated.
As it is shown in figure 1, the detailed description of the invention of the present invention is: build a kind of circulating tumor cell automatic separation system, bag
Including erythrocyte and crack unit A, circulating tumor cell separative element B, circulating tumor cell collector unit C, described erythrocyte cracks
Unit A, described circulating tumor cell separative element B and described circulating tumor cell collector unit C are sequentially connected with, described erythrocyte
Cracking addition erythrocyte in unit A and crack liquid and blood, erythrocyte cracks liquid and is cracked by the erythrocyte in blood, and described circulation is swollen
Oncocyte separative element B includes that circulating line 5, cell separation module 6, circulation control module 4, described circulation control module 4 are pacified
Being contained on described circulating line 5, described cell separation module 6 is arranged on described circulating line 5, described circulation control module 4
Controlling blood to circulate in described circulating line 5, described cell separation module 6 is by blood white in described circulating line 5
Cell filtration, the circulating tumor cell that described circulating line 5 is flowed out by described circulating tumor cell collector unit C is collected.Specifically
In embodiment, described circulation control module 4 is blood pump, implements to circulate by blood pump, and by described circulation control
Molding block 4 can realize flow speed control, controls the separation process of circulating tumor cell the most more easily.
As shown in Figure 1 and Figure 2, the specific implementation process of the present invention is: described erythrocyte cracks unit and includes blood vessel 1,
Described erythrocyte cracks and adds erythrocyte in the blood vessel 1 of unit A and crack liquid and blood, and erythrocyte cracks liquid by blood
Erythrocyte cracks, and the blood after erythrocyte cracks enters in described circulating tumor cell separative element B, circulation control module 4
Control control blood circulate in described circulating line 5, when blood flow to described cell separation module 6, described carefully
Born of the same parents' separation module 6 arranges leukoreduction filter net 17, and the leukoreduction filter net 17 of described cell separation module 6 is by described circulating line
The leukoreduction filter of blood in 5, the tissue fluid after filtration is flowed in container 18.Owing to circulating tumor cell volume is relatively big, because of
This, circulating tumor cell still circulates in described circulating line 5, so that leukoreduction filter is cleaner.When having filtered
Cheng Hou, described circulating line 5 opens with described circulating tumor cell collector unit C junction, and described circulating tumor cell is collected
Unit C collects the circulating tumor cell that described circulating line 5 flows out.
As it is shown in figure 1, the preferred embodiment of the present invention is: described circulating line 5 is circulating line, and blood is at circulating pipe
Road circulates, the convenient circulation of circulating line, and can be easily separated.
As it is shown in figure 1, the preferred embodiment of the present invention is: described erythrocyte cracks cell S and described circulating tumor is thin
First shutoff valve 3 is set between born of the same parents separative element B.In blood entry port, add erythrocyte and crack liquid and blood, the first shutoff valve
3 close, and erythrocyte cracks liquid and cracked by the erythrocyte in blood, and after having cracked, the first shutoff valve 3 is opened, and erythrocyte cracks
After blood flow into described circulating tumor cell separative element B.
As it is shown in figure 1, the preferred embodiment of the present invention is: described circulating line 5 also sets up the 3rd shutoff valve 9, described
Between circulating tumor cell separative element B and described circulating tumor cell collector unit C, the second shutoff valve 10 is set.At described ring
When blood in flow tube road 5 flows, the 3rd shutoff valve 9 is opened, and the second shutoff valve 10 cuts out, it is achieved in described circulating line 5
Blood circulation flows.When blood in described circulating line 5 flows, the second shutoff valve 10 is opened, and the 3rd shutoff valve 9 cuts out,
The circulating tumor cell separated in described circulating line 5 flows in described circulating tumor cell collector unit C.
As in figure 2 it is shown, the preferred embodiment of the present invention is: also include that reflux line 7, described cell separation module 6 wrap
Including and also include collecting the container 18 of tissue fluid, tissue fluid drainage screen 19 and filter chamber 16, tissue fluid drainage screen 19 was arranged on
In filter chamber 16, filter chamber 16 is socketed on described circulating line 5, and described container 18 is by described tissue fluid drainage screen 19 and described
Reflux line 7 connects described circulating line 5.Tissue fluid after being filtered by reflux line 7 passes through tissue fluid drainage screen 19 times
Flow to described circulating line 5, proceed to circulate, during circulating, leukocyte is filtered more up hill and dale clean,
Leave circulating tumor cell, it is achieved the separation of circulating tumor cell.
As it is shown in figure 1, the preferred embodiment of the present invention is: described circulating tumor cell collector unit C includes that negative pressure is thin
Born of the same parents' collection module 15, described circulating tumor cell collector unit C also includes that tumor cell marking fluid adds entrance 11 and cytometer
Digital-to-analogue block 13, adds entrance 11 from tumor cell marking fluid and adds tumor cell marking fluid, makes what described circulating line 5 flow through to follow
Ring tumor cell is labeled.Negative pressure cell collection module 15, by negative pressure, makes circulating tumor cell to negative pressure cell collection module
Move in 15, counted by cell counting module 13.
As it is shown in figure 1, the preferred embodiment of the present invention is: described circulation control module 4 connects described erythrocyte and cracks
Unit A and described circulating line 5.Described circulation control module 4 directly receives described erythrocyte and cracks the blood after unit A processes
Liquid, flows into described circulating line 5, more convenient control by circulation control module 4 the most again.
The solution have the advantages that: build a kind of circulating tumor cell automatic separation system, crack list including erythrocyte
Unit A, circulating tumor cell separative element B, circulating tumor cell collector unit C, described erythrocyte cracks unit A, described circulation
Tumor cell separative element B and described circulating tumor cell collector unit C is sequentially connected with, and described erythrocyte cracks in unit A and adds
Entering erythrocyte and crack liquid and blood, erythrocyte cracks liquid and is cracked by the erythrocyte in blood, and described circulating tumor cell separates single
Unit B includes that circulating line 5, cell separation module 6, circulation control module 4, described circulation control module 4 are arranged on described circulation
On pipeline 5, described cell separation module 6 is arranged on described circulating line 5, and described circulation control module 4 controls blood in institute
Stating in circulating line 5 and circulate, described cell separation module 6 is by the leukoreduction filter of blood in described circulating line 5, described
The circulating tumor cell that described circulating line 5 is flowed out by circulating tumor cell collector unit C is collected.One circulating tumor of the present invention
Cell automatic separation system, implements the process that the integration to circulating tumor cell is automatically separated, improves circulating tumor thin
The separation efficiency of born of the same parents, and the intact of cell can be kept well in separation process, conveniently it is analyzed.
Above content is to combine concrete preferred implementation further description made for the present invention, it is impossible to assert
Being embodied as of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of present inventive concept, it is also possible to make some simple deduction or replace, all should be considered as belonging to the present invention's
Protection domain.
Claims (10)
1. a circulating tumor cell automatic separation system, it is characterised in that include that erythrocyte cracks unit, circulating tumor cell
Separative element, circulating tumor cell collector unit, described erythrocyte cracks unit, described circulating tumor cell separative element and institute
Stating circulating tumor cell collector unit to be sequentially connected with, described erythrocyte cracks addition erythrocyte in unit and cracks liquid and blood, red
Cell cracks liquid and is cracked by the erythrocyte in blood, and described circulating tumor cell separative element includes circulating line, cell separation
Module, circulation control module, described circulation control module is arranged on described circulating line, and described cell separation module is arranged on
On described circulating line, described circulation control module controls blood and circulates in described circulating line, described cell separation
Module is by the leukoreduction filter of blood in described circulating line, and described circulating tumor cell collector unit is by described circulating line stream
The circulating tumor cell gone out is collected.
The most described circulating tumor cell automatic separation system, it is characterised in that described cell separation mould
Block includes leukoreduction filter net.
The most described circulating tumor cell automatic separation system, it is characterised in that described circulating line is
Circulating line.
The most described circulating tumor cell automatic separation system, it is characterised in that described erythrocyte cracks
Between unit and described circulating tumor cell separative element, the first shutoff valve is set.
The most described circulating tumor cell automatic separation system, it is characterised in that described circulating tumor is thin
Between born of the same parents' separative element and described circulating tumor cell collector unit, the second shutoff valve is set.
The most described circulating tumor cell automatic separation system, it is characterised in that also include return duct
Road, described cell separation module includes container and the tissue fluid drainage screen also including collecting tissue fluid, and described container passes through institute
State tissue fluid drainage screen and described reflux line connects described circulating line.
The most described circulating tumor cell automatic separation system, it is characterised in that described ring flow control module
Block is blood pump.
The most described circulating tumor cell automatic separation system, it is characterised in that described circulating tumor is thin
Born of the same parents' collector unit includes negative pressure cell collection module.
The most described circulating tumor cell automatic separation system, it is characterised in that described ring flow control module
Block connects described erythrocyte and cracks unit and described circulating line.
The most described circulating tumor cell automatic separation system, it is characterised in that also include that tumor is thin
Born of the same parents' marking fluid adds entrance.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610613548.2A CN106085832A (en) | 2016-07-29 | 2016-07-29 | A kind of circulating tumor cell automatic separation system |
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| CN201610613548.2A CN106085832A (en) | 2016-07-29 | 2016-07-29 | A kind of circulating tumor cell automatic separation system |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106769693A (en) * | 2016-11-14 | 2017-05-31 | 中国科学院重庆绿色智能技术研究院 | A kind of circulating tumor cell automatic checkout system based on Raman spectrum |
| CN107988042A (en) * | 2017-11-27 | 2018-05-04 | 长春理工大学 | A kind of circulating tumor cell separator and its detection method |
| CN112111385A (en) * | 2020-08-19 | 2020-12-22 | 东南大学 | Cancer cell sorting and detecting device |
| WO2021056952A1 (en) * | 2019-09-27 | 2021-04-01 | 东南大学 | Leukocyte extraction device |
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| CN106769693A (en) * | 2016-11-14 | 2017-05-31 | 中国科学院重庆绿色智能技术研究院 | A kind of circulating tumor cell automatic checkout system based on Raman spectrum |
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| CN112111385A (en) * | 2020-08-19 | 2020-12-22 | 东南大学 | Cancer cell sorting and detecting device |
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