CN106075956A - A kind of platelet rich plasma PRP extracts centrifugal separating device and using method thereof - Google Patents
A kind of platelet rich plasma PRP extracts centrifugal separating device and using method thereof Download PDFInfo
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- CN106075956A CN106075956A CN201610365011.9A CN201610365011A CN106075956A CN 106075956 A CN106075956 A CN 106075956A CN 201610365011 A CN201610365011 A CN 201610365011A CN 106075956 A CN106075956 A CN 106075956A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
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Abstract
The present invention provides a kind of PRP to extract centrifugal separating device and using method thereof, PRP extracts centrifugal separating device and includes the first centrifuge bottle and the second centrifuge bottle, second centrifuge bottle is unsettled to be nested in the first centrifuge bottle, first centrifuge bottle and the second centrifuge bottle are connected with tee pipe coupling by the first check valve and the second check valve, and the control of the first check valve and the second check valve flows to contrary；First centrifuge bottle bottle cap is provided with centrifuge bottle nesting mouth, the second conduit mouth；Centrifuge bottle nesting mouth is positioned at the center of the first centrifuge bottle；Conduit in second conduit mouth is connected with tee pipe coupling by the first check valve；Second centrifuge bottle bottle cap is provided with the second blow vent, the second blood plasma inlet and the 3rd conduit mouth；The conduit of the second blood plasma inlet is connected with tee pipe coupling by the second check valve.The PRP using the present invention to provide extracts centrifugal separating device, blood plasma can be carried out secondary centrifuging, it is not necessary to transfer blood, will not be contaminated.
Description
Technical field
The present embodiments relate to technical field of medical instruments, particularly to a kind of PRP extract centrifugal separating device and
Using method.
Background technology
Platelet rich plasma (Platelet Rich Plasma, be called for short PRP) be obtained by centrifugal blood containing height
The thrombocyte plasma of concentration.The platelet of human body, in addition to can providing cohesion stopping blooding when, also contains in platelet
Have much the most relevant with wound healing and bone regeneration multiple somatomedin, such as platelet derived growth factor, convert grow because of
Son, insulin like growth factor, epidermal growth factor and VEGF etc..Somatomedin in platelet,
Differentiation and propagation to cell play facilitation, and these somatomedin have cooperative response each other, promote thin with other
The factor interaction of cytoactive and impact, maintain the balance of organizational environment jointly, to wound healing, repairs and regeneration has
Important effect.Further, since PRP can extract from patient's self-blood, after extracted enrichment processes, can be used for disease of being correlated with
Sick treatment, thus abundance, draw materials conveniently, and preparation method is simple and health can be allowed to absorb.Self PRP uses and can keep away
The problem exempting from virus disseminating and immunologic rejection occurs.Therefore during PRP is widely applied to different medical domains.Such as orthopaedics, mouth
Chamber section, decorative sursery, sports medical science and aesthetic medicine.
At present, there is no specialty and list for the equipment separated that extracts of PRP, mostly use open manual secondary centrifuging
Method prepares PRP, but the following shortcoming existed strongly limit it in clinical application: be 1. easily subject to outside contamination, Duo Gerong
Device shifts, adds platelet activation and contaminated chance；2. recovery rate of blood platelet is relatively low；3. easily by the shadow of operation factors
Ringing, preparation method is not sufficiently stable；4. there is no special application tool, in-convenience in use.
Summary of the invention
For solving problem, the present invention provides a kind of platelet rich plasma PRP to extract centrifugal separating device, is centrifuged including first
Bottle and the second centrifuge bottle, described second centrifuge bottle is unsettled to be nested in described first centrifuge bottle, described first centrifuge bottle and described
Second centrifuge bottle is connected by tee pipe coupling, is provided with the first check valve and the second check valve, institute in described tee pipe coupling
The control stating the first check valve and described second check valve flows to contrary；
Described first centrifuge bottle bottle cap is provided with the first blow vent, centrifuge bottle nesting mouth, the first blood plasma inlet, first leads
The mouth of pipe and the second conduit mouth；Described centrifuge bottle nesting mouth is positioned at the center of described first centrifuge bottle, for nesting place second from
Heart bottle；Conduit in described second conduit mouth is connected with described tee pipe coupling conducting by the first check valve；
Described second centrifuge bottle bottle cap is provided with the second blow vent, the second blood plasma inlet and the 3rd conduit mouth；Described
The conduit of two blood plasma inlets is connected with described tee pipe coupling conducting by the second check valve.
Further, described first and second check valves are provided with liquid feeding end and liquid outlet；Described liquid feeding end with described go out liquid
End is isolated by funnel-form interlayer, and the beaker flask of described funnel-form interlayer is positioned at described liquid outlet；It is provided with logical bottom described beaker flask
Hole, makes described liquid feeding end be connected with described liquid outlet；Described liquid outlet is provided with drainage screen, before being positioned at described funnel-form interlayer
Side；Being provided with lightweight bead between described beaker flask and described drainage screen, the diameter of described lightweight bead is straight more than described through hole
Footpath；
The liquid feeding end of described first check valve is connected with the conduit in described second conduit mouth；Going out of described first check valve
Liquid end is connected with the first interface of described tee pipe coupling；
The liquid feeding end of described second check valve is connected with the second interface of described tee pipe coupling；Described second check valve
Liquid outlet is connected with described second blood plasma inlet.
Further, all use between described conduit, check valve and tee pipe coupling hose connection.
Further, it is the coniform of closing bottom described first centrifuge bottle and the second centrifuge bottle.
Further, the 3rd interface of described tee pipe coupling is provided with storage area ball.
Further, described centrifuge bottle nesting mouth, the first conduit mouth, the second conduit mouth and the 3rd conduit mouth are provided with close
Envelope rubber ring.
The present invention also provides for a kind of any one PRP as mentioned above and extracts the using method of centrifugal separating device, including:
To inject the first centrifuge bottle from the first blood plasma inlet containing anticoagulant fresh blood, by described PRP extract from
Heart segregation apparatus is placed in a centrifuge and carries out being centrifuged for the first time；
After being centrifuged for the first time, blood completes layering, uses syringe to be in by the conduit extraction of the first conduit mouth
The red blood cell layer of bottom；
When syringe extraction to red blood cell layer is with when being under the PRP demarcation line in intermediate layer, use syringe by the
Two conduit mouth conduit extraction PRP layer liquid, until extraction to PRP layer be on the stillness of night layer demarcation line on upper strata；
PRP layer liquid in syringe is injected in the second centrifuge bottle by the second blood plasma inlet, by PRP extract from
It is centrifugal that heart segregation apparatus is again placed in centrifuge carrying out second time；
After secondary centrifuging completes, extract the bottom red blood cell layer in the second centrifuge bottle with syringe；
When syringe extraction to red blood cell layer is with PRP layer demarcation line, exchanges new syringe for and received by the 3rd conduit mouth
Collect the PRP layer in the second centrifuge bottle.
Further, after being centrifuged for the first time, use the syringe conduit extraction PRP layer liquid by the second conduit mouth
When the second centrifuge bottle injects, again inject containing anticoagulant fresh blood to the first centrifuge bottle.
Further, during PRP layer in collecting the second centrifuge bottle after secondary centrifuging, can take out according to actual needs
Take the different amounts of stillness of night, be mixedly configured into variable concentrations PRP.
The PRP using the present invention to provide extracts centrifugal separating device, blood plasma can carry out secondary centrifuging, and carrying out two
Secondary centrifugal time, it is not necessary to be transferred to blood, outside centrifuge bottle, directly shift inside device, will not be contaminated；Another
Aspect, second time is to carry out in less centrifuge bottle time centrifugal, and PRP layer is obvious with the demarcation line of red blood cell layer, easily takes
Go out rich platelet blood.The PRP using the present invention to provide extracts the using method of centrifugal separating device, the rich platelet blood of extraction
Slurry concentration is high, will not be contaminated.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is this
Some bright embodiments, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to
Other accompanying drawing is obtained according to these accompanying drawings.
The PRP that Fig. 1 provides for the present invention extracts centrifugal separating device structural representation；
Fig. 2 is the first centrifuge bottle Bottle cap structure schematic diagram in Fig. 1；
Fig. 3 is the second centrifuge bottle Bottle cap structure schematic diagram in Fig. 1；
Fig. 4 is one-way valve structures schematic diagram in Fig. 1；
Fig. 5 is filter screen structure schematic diagram in Fig. 4；
Fig. 6 A is check valve schematic diagram in PRP extracts centrifugal separating device use state one；
Fig. 6 B is check valve schematic diagram in PRP extracts centrifugal separating device use state two；
Fig. 7 is another preferred embodiment schematic diagram of tee pipe coupling in Fig. 1；
Fig. 8 is the layering schematic diagram of centrifugal rear blood.
Reference:
10 first centrifuge bottle 20 second centrifuge bottle 30 tee pipe couplings
41 first check valve 42 second check valve 101 first blow vents
102 centrifuge bottle nesting mouth 103 first blood plasma inlet 104 first conduit mouth
105 second conduit mouth 201 second blow vent 202 second blood plasma blow vents
203 the 3rd conduit mouth 301 first interface 302 second interfaces
303 the 3rd interface 304 storage area ball 401 liquid feeding ends
402 liquid outlet 403 beaker flask 404 through holes
405 drainage screen 406 lightweight beads
Detailed description of the invention
For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
The a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under not making creative work premise, broadly falls into the scope of protection of the invention.
The embodiment of the present invention provides a kind of PRP to extract centrifugal separating device, sees Fig. 1～Fig. 3, and this PRP extracts centrifugal point
Including the first centrifuge bottle 10 and the second centrifuge bottle 20 from device, described second centrifuge bottle 20 is unsettled is nested in described first centrifuge bottle
In 10, described first centrifuge bottle 10 is connected, in described tee pipe coupling by tee pipe coupling 30 with described second centrifuge bottle 20
30 are provided with the first check valve 41 and the second check valve 42, described first check valve 41 and the control stream of described second check valve 42
To on the contrary；
As in figure 2 it is shown, the first centrifuge bottle 10 bottle cap is provided with the first blow vent 101, centrifuge bottle nesting mouth the 102, first blood
Slurry inlet the 103, first conduit mouth 104 and the second conduit mouth 105；
Wherein: the effect of regulation air pressure when the first blow vent 101 effect is extraction and injects liquid；
Centrifuge bottle nesting mouth 102 is for placing the second centrifuge bottle 20；
First blood plasma inlet 103 is for injecting fresh blood in the first centrifuge bottle 10；
Conduit passes the first conduit mouth 104 and the second conduit mouth 105, and extends to the bottom of the first centrifuge bottle 10, and first
The conduit of conduit mouth 104 has been centrifuged the red blood cell layer of rear first centrifuge bottle 10 bottom for extracting；Second conduit mouth 105
Conduit is for extracting the PRP (after the conduit extraction red blood cell layer of the first conduit mouth 104) in residue intermediate layer, and passes through T joint
30 are delivered in the second centrifuge bottle 20.
As it is shown on figure 3, the second centrifuge bottle 20 bottle cap is provided with second blow vent the 201, second blood plasma inlet 202 and the 3rd
Conduit mouth 203, wherein:
The effect of regulation air pressure when second blow vent 201 effect is extraction and injects liquid；
The conduit of the second blood plasma inlet 202 by described tee pipe coupling 30 conducting connect, for input from first from
The PRP of heart bottle 10 extraction；
Conduit passes the 3rd conduit mouth 203, and extends to the bottom of the second centrifuge bottle 20, be used for extracting centrifugal after second from
Red blood cell layer bottom heart bottle 20.
When being embodied as, the check valve in above-described embodiment can be as shown in Figure 4: the first check valve 41 and the second check valve
42 are provided with liquid feeding end 401 and liquid outlet 402, and liquid feeding end 401 is isolated by funnel-form interlayer with liquid outlet 402.Funnel-form interlayer
Beaker flask 403 is positioned at liquid outlet 402.It is provided with through hole 404 bottom beaker flask 403, makes liquid feeding end 401 be connected with liquid outlet 402.
Liquid outlet 402 is provided with drainage screen 405 (as shown in Figure 5), is positioned at funnel-form interlayer front；Between beaker flask 403 and drainage screen 405
Being provided with lightweight bead 406, the diameter of lightweight bead 406 is more than the diameter of through hole 404.When air pressure or hydraulic pressure are from liquid outlet 402
When putting on beaker flask 403, lightweight bead 406 is adjacent to through hole 404, liquid or gas and will not flow out from through hole 404.Work as air pressure
Or hydraulic pressure is when liquid feeding end 401 puts on through hole 404, lightweight bead 406 can float, and is no longer adjacent to through hole 404, liquid or
Gas can enter liquid outlet 402 from through hole 404.Drainage screen 405 1 aspect is used for preventing lightweight bead 406 by gas or liquid
Body rushes to check valve, and on the other hand, liquid or gas can pass through by drainage screen 405 smoothly.
Conduit and the liquid feeding end 401 of the first check valve 41 in second conduit mouth 105 connect, the first check valve 41 go out liquid
End 402 is connected with the first interface 301 of tee pipe coupling 30.Second blood plasma inlet 202 and the liquid outlet of the second check valve 42
402 connect, and the liquid feeding end 401 of the second check valve 42 is connected with the second interface 302 of tee pipe coupling 30.
When being embodied as, when with three interface 303 of syringe connecting tee pipe joint 30, by the second conduit mouth
When conduit on 105 extracts the blood within the first centrifuge bottle 10, the lightweight bead 406 in the first check valve 41 can float, no
Being adjacent to through hole 404 again, meanwhile, the lightweight bead 406 of the second check valve 42 can be held is adjacent to through hole 404, and blood will not
Entering the second centrifuge bottle 20 by the second check valve 42, therefore, blood can only be from the first check valve 41 by the first check valve 41
And tee pipe coupling 30 enters syringe inside (as shown in Figure 6A).
When the outside injecting blood of syringe, the lightweight bead 406 of the first check valve 41 compacted can be adjacent to through hole 404,
Blood will not enter the first centrifuge bottle 10 by the first check valve 51, and meanwhile, blood is pressed downward against the light of the second check valve 42
Matter bead 406, lightweight bead 406 is no longer adjacent to through hole 404, and blood can only enter the second centrifuge bottle 20 from the second check valve 42
(as shown in Figure 6B).
Preferably, the conduit in the second conduit mouth 105 and the first check valve 41, the first check valve 41 and tee pipe coupling
30, tee pipe coupling 30 and the second check valve 42, all use flexible pipe even between the second check valve 42 and the second blood plasma inlet 202
Connect.Use hose connection conduit, check valve and tee pipe coupling 30, easy to operate, good airproof performance, will not leakage.
It is preferred that be coniform bottom the first centrifuge bottle 10 and the second centrifuge bottle 20.Use coniform bottom, coniform
Bottom makes bottom liquid relatively concentrate, when extracting the blood within centrifuge bottle, it is possible to more completely within extraction centrifuge bottle
Blood.
It is preferred that the 3rd interface 303 of tee pipe coupling 30 is provided with storage area ball 304 (as shown in Figure 7), storage area
Ball 304 is easy to temporarily deposit blood.Because PRP is the most less, storage area ball can be set according to the specification of the first centrifuge bottle
Volume size, the PRP separated can reach to deposit the first centrifuge bottle is advisable, so can without PRP is drawn in syringe,
Have only to be cached to storage area ball.
It is preferred that conduit, centrifuge bottle, check valve, tee pipe coupling 30 and storage empty 304 all use transparent materials to make,
It is easy to the blood situation of real-time monitored device interior.
It is preferred that centrifuge bottle nesting mouth the 102, first conduit mouth the 104, second conduit mouth 105 and the 3rd conduit mouth 203 are all provided with
It is equipped with sealing rubber ring.Each conduit mouth on centrifuge bottle bottle cap, centrifuge bottle nesting mouth 102 is respectively provided with sealing rubber ring (doctor
With), it is simple to conduit and the installation of centrifuge bottle and fixing, and good sealing effectiveness can be reached.
The present invention also provides for a kind of any one PRP as mentioned above and extracts the using method of centrifugal separating device, specifically real
Shi Shi:
To inject the first centrifuge bottle from the first blood plasma inlet containing anticoagulant fresh blood, by described PRP extract from
Heart segregation apparatus is placed in a centrifuge and carries out being centrifuged for the first time；
After being centrifuged for the first time, blood completes layering, uses syringe to be in by the conduit extraction of the first conduit mouth
The red blood cell layer of bottom；As shown in Figure 8, being generally divided into three layers after centrifugal blood, bottom is red blood cell layer, and intermediate layer is PRP layer,
The superiors are stillness of night layer；Wherein, supernatant layer is a with the demarcation line of intermediate layer PRP layer, red blood cell layer and the PRP in intermediate layer
The demarcation line of layer is b；
When syringe extraction to red blood cell layer is with when being under the PRP layer demarcation line b in intermediate layer, syringe is used to pass through
Second conduit mouth conduit extraction PRP layer liquid, until extraction to PRP layer be on the stillness of night layer demarcation line a on upper strata；This
Time, the liquid of extraction just contains and mixes unclear liquid between PRP and PRP with the stillness of night, erythrocyte；
Extraction in syringe is injected in the second centrifuge bottle by the second blood plasma inlet night, PRP is extracted centrifugal
It is centrifugal that segregation apparatus is again placed in centrifuge carrying out second time；
After two times centrifugal, extract the bottom red blood cell layer in the second centrifuge bottle with syringe；
When syringe extraction to red blood cell layer is with PRP layer demarcation line, exchanges new syringe for and received by the 3rd conduit mouth
Collect the PRP layer in the second centrifuge bottle.
The PRP using the embodiment of the present invention to provide extracts centrifugal separating device, and the method extracting PRP is compared to traditional
Manual collection method, has purity height, collects free of contamination feature, specifically:
Traditional centrifuge bottle general requirements is relatively big, and PRP layer is because amount is fewer, so with red on centrifuge bottle after Li Xin
Cell once, the boundary of stillness of night layer unclear, general being difficult to extracts PRP layer accurately.If by PRP layer and red blood cell layer, the stillness of night
Layer boundary extracts in the lump, purity will be caused the highest with erythrocyte；If in order to reach certain purity, avoiding boundary
Extraction, then can cause the PRP extracted to measure very little, and be not easily controlled；And the PRP that the embodiment of the present invention provides extracts centrifugation
Device, fractional centrifugation, when for the first time centrifugal, PRP layer and with red blood cell layer, stillness of night layer boundary mixed liquor in the lump will be included
It is input to the second centrifuge bottle, it is ensured that all of PRP layer is included, after secondary centrifuging, due to the PRP layer institute at the second centrifuge bottle
Accounting rate is higher, and the second centrifuge bottle is because specification is less, and scale is the most more apparent, is therefore easy for energy control, extracts pure
Spend the highest PRP；
And during the PRP of embodiment of the present invention offer extracts centrifugal separating device intermediary operation, owing to whole process is in close
Seal dustless state, be not result in that antibacterial infects；
It is preferred that to the second centrifuge bottle inject extract time, inject containing anticoagulant fresh blood to the first centrifuge bottle；
By described PRP extraction centrifugal separating device be placed in a centrifuge, simultaneously in the first centrifuge bottle containing anticoagulant fresh blood
It is centrifuged with the extract in the second centrifuge bottle, it is possible to achieve concurrent working, improves extraction efficiency.
It is preferred that during PRP layer in collecting the second centrifuge bottle after secondary centrifuging, can extract according to actual needs
The different amounts of stillness of night, it is configured to variable concentrations PRP, meets different demands.
Although the most more employs such as the first centrifuge bottle, the second centrifuge bottle, the first check valve, second is unidirectional
Valve, tee pipe coupling, the first blow vent, the first blood plasma inlet, centrifuge bottle nesting hole, the second conduit mouth, the second blow vent, the
Two blood plasma blow vents, the 3rd conduit mouth, first interface, the second interface, the 3rd interface, storage area ball, liquid feeding end, liquid outlet,
Beaker flask, through hole, drainage screen, the term such as lightweight bead, but it is not precluded from using the probability of other term.Use these terms
It is only used to describe and explain more easily the essence of the present invention；Be construed as any additional restriction be all with
Spirit of the present invention is contrary.
Last it is noted that various embodiments above is only in order to illustrate technical scheme, it is not intended to limit；To the greatest extent
The present invention has been described in detail by pipe with reference to foregoing embodiments, it will be understood by those within the art that: it depends on
So the technical scheme described in foregoing embodiments can be modified, or the most some or all of technical characteristic is entered
Row equivalent；And these amendments or replacement, do not make the essence of appropriate technical solution depart from various embodiments of the present invention technology
The scope of scheme.
Claims (9)
1. a platelet rich plasma PRP extracts centrifugal separating device, it is characterised in that: include the first centrifuge bottle (10) and second
Centrifuge bottle (20), described second centrifuge bottle (20) is unsettled to be nested in described first centrifuge bottle (10), described first centrifuge bottle
(10) connected by tee pipe coupling (30) with described second centrifuge bottle (20), described tee pipe coupling (30) is provided with first
Check valve (41) and the second check valve (42), the control of described first check valve (41) and described second check valve (42) flows to phase
Instead；
Described first centrifuge bottle (10) bottle cap is provided with the first blow vent (101), centrifuge bottle nesting mouth (102), the first blood plasma note
Entrance (103), the first conduit mouth (104) and the second conduit mouth (105)；Described centrifuge bottle nesting mouth (102) is positioned at described first
The center of centrifuge bottle (10), places the second centrifuge bottle (20) for nesting；Conduit on described second conduit mouth (105) is by the
One check valve (41) is connected with described tee pipe coupling (30) conducting；
Described second centrifuge bottle (20) bottle cap is provided with the second blow vent (201), the second blood plasma inlet (202) and the 3rd conduit
Mouth (203)；The conduit of described second blood plasma inlet (202) is led with described tee pipe coupling (30) by the second check valve (42)
Lead to connect.
PRP the most according to claim 1 extracts centrifugal separating device, it is characterised in that: described first and second check valves
(41,42) are provided with liquid feeding end (401) and liquid outlet (402)；Described liquid feeding end (401) and described liquid outlet (402) are by funnel-form
Interlayer is isolated, and the beaker flask (403) of described funnel-form interlayer is positioned at described liquid outlet (402)；Described beaker flask (403) bottom sets
There is through hole (404), make described liquid feeding end (401) be connected with described liquid outlet (402)；Described liquid outlet (402) is provided with filtration
Net (405), is positioned at described funnel-form interlayer front；It is provided with lightweight little between described beaker flask (403) and described drainage screen (405)
Ball (406), the diameter of described lightweight bead (406) is more than the diameter of described through hole (404)；
The liquid feeding end (401) of described first check valve (41) is connected with the conduit on described second conduit mouth (105)；Described first
The liquid outlet (402) of check valve (41) is connected with the first interface (301) of described tee pipe coupling (30)；
The liquid feeding end (401) of described second check valve (42) is connected with second interface (302) of described tee pipe coupling (30)；Institute
The liquid outlet (302) stating the second check valve (42) is connected with described second blood plasma inlet (202).
PRP the most according to claim 1 extracts centrifugal separating device, it is characterised in that: described conduit, check valve and three
All use hose connection between connection for bbreather pipe.
PRP the most according to claim 1 extracts centrifugal separating device, it is characterised in that: described first centrifuge bottle (10) and
Second centrifuge bottle (20) bottom is coniform for close.
PRP the most according to claim 1 extracts centrifugal separating device, it is characterised in that: described tee pipe coupling (30)
3rd interface (303) is provided with storage area ball (304).
PRP the most according to claim 1 extracts centrifugal separating device, it is characterised in that: described centrifuge bottle nesting mouth
(102), the first conduit mouth (104), the second conduit mouth (105) and the 3rd conduit mouth (203) are provided with sealing rubber ring.
7. use the PRP according to any one of claim 1～6 to extract a using method for centrifugal separating device, its feature
It is, including:
To inject the first centrifuge bottle from the first blood plasma inlet containing anticoagulant fresh blood, described PRP will be extracted centrifugal point
It is placed in a centrifuge from device and carries out being centrifuged for the first time；
After being centrifuged for the first time, blood completes layering, uses syringe to be in bottom by the conduit extraction of the first conduit mouth
Red blood cell layer；
When syringe extraction to red blood cell layer is with when being under the PRP layer demarcation line in intermediate layer, use syringe by second
Conduit mouth conduit extraction PRP layer liquid, until extraction to PRP layer be on the stillness of night layer demarcation line on upper strata；
PRP layer liquid in syringe is injected in the second centrifuge bottle by the second blood plasma inlet, PRP is extracted centrifugal point
It is again placed in centrifuge carrying out second time from device centrifugal；
After secondary centrifuging completes, extract the bottom red blood cell layer in the second centrifuge bottle with syringe；
When syringe extraction is to red blood cell layer and PRP layer demarcation line, exchanges new syringe for and collect the by the 3rd conduit mouth
PRP layer in two centrifuge bottles.
PRP the most according to claim 7 extracts the using method of centrifugal separating device, it is characterised in that: the most centrifugal
After completing, use syringe by the conduit of the second conduit mouth extraction PRP layer liquid when the second centrifuge bottle injects, again to the
One centrifuge bottle injects containing anticoagulant fresh blood.
PRP the most according to claim 7 extracts the using method of centrifugal separating device, it is characterised in that: collecting secondary
During PRP layer in the second centrifuge bottle after Li Xin, the different amounts of stillness of night can be extracted, is mixedly configured into not according to actual needs
With concentration PRP.
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| CN201610365011.9A CN106075956B (en) | 2016-05-27 | 2016-05-27 | Platelet-rich blood plasma PRP extraction and centrifugation apparatus and use method thereof |
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| CN201610365011.9A CN106075956B (en) | 2016-05-27 | 2016-05-27 | Platelet-rich blood plasma PRP extraction and centrifugation apparatus and use method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106994396A (en) * | 2017-05-23 | 2017-08-01 | 武汉朗克医疗器械有限公司 | PRP continuous centrifugal separation devices and separation method |
| CN110251995A (en) * | 2019-06-17 | 2019-09-20 | 郭钦 | A kind of economic preparation method of platelet rich plasma |
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| CN106994396B (en) * | 2017-05-23 | 2019-03-29 | 武汉朗克医疗器械有限公司 | PRP continuous centrifugal separation device and separation method |
| CN110251995A (en) * | 2019-06-17 | 2019-09-20 | 郭钦 | A kind of economic preparation method of platelet rich plasma |
| CN110251995B (en) * | 2019-06-17 | 2021-05-14 | 郭钦 | Economical preparation method of platelet-rich plasma |
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