CN206736237U - A kind of filter for removing excretion body - Google Patents
A kind of filter for removing excretion body Download PDFInfo
- Publication number
- CN206736237U CN206736237U CN201720465148.1U CN201720465148U CN206736237U CN 206736237 U CN206736237 U CN 206736237U CN 201720465148 U CN201720465148 U CN 201720465148U CN 206736237 U CN206736237 U CN 206736237U
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- China
- Prior art keywords
- filter
- outer tube
- excretion body
- pipe
- filtering group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 230000029142 excretion Effects 0.000 title claims abstract description 49
- 238000001914 filtration Methods 0.000 claims abstract description 40
- 239000011148 porous material Substances 0.000 claims abstract description 7
- 230000004323 axial length Effects 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 9
- 238000011144 upstream manufacturing Methods 0.000 claims description 7
- 229920002492 poly(sulfone) Polymers 0.000 claims description 6
- 230000010148 water-pollination Effects 0.000 claims description 6
- 238000010276 construction Methods 0.000 abstract description 2
- 210000002966 serum Anatomy 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 7
- 239000011324 bead Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 4
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 210000001163 endosome Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 210000002487 multivesicular body Anatomy 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004413 cardiac myocyte Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000012202 endocytosis Effects 0.000 description 1
- 210000001808 exosome Anatomy 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 230000005660 hydrophilic surface Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 230000034217 membrane fusion Effects 0.000 description 1
- 239000013618 particulate matter Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- External Artificial Organs (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
It the utility model is related to a kind of filter for removing excretion body, it mainly includes an outer tube and filtering group, wherein, the pipe interior diameter of outer tube is 8cm~16cm, the axial length of outer tube is 30cm~50cm, filtering group includes several hollow filter pipes, and several hollow filter pipes are axially parallel arranged in outer tube along outer tube, and the pipe interior diameter of each hollow filter pipe is 150 μm~250 μm, the pore size filter of tube wall is 2nm~4nm；Space is occupied by hollow filter pipe in 60%~80% pipe of outer tube；Filtering group has an inlet end portion and an outlet end, and the position of the outlet end of the close filtering group of outer tube offers an outlet.The filter of the present utility model for removing excretion body, it is simple in construction, larger filter area can be provided, high-volume sample operations can be carried out, and may be reused.
Description
Technical field
A kind of filter for removing excretion body is the utility model is related to, belongs to biological technical field.
Background technology
Eukaryotic can carry out endocytosis and form endosome (endosome), re-form multivesicular body
(multivesicular bodies, MVBs), it is with that after cell membrane fusion, can discharge nano level duplicature vesicles to carefully
In extracellular environment, this duplicature vesicles are referred to as excretion body (exosomes).
Although excretion body has just been found in the eight zero years generation in last century, it is mistaken as simply a kind of always in the past
The discarded object of cell.Several years up to date, scientist had found the albumen containing cell-specific, lipid and core in this small vesica
Acid, other cells can be passed to as signaling molecule so as to change the function of other cells.The excretion body of different cell secretions, meeting
With the distinctive molecule of mother cell, therefore there is no constituent and function.
The size of excretion body is about 30-150nm, and the content rough estimate of excretion body in blood is 3,000,000/ml, so
Serum for cell culture also contains substantial amounts of excretion body.Existing document points out that the excretion in serum knows from experience the table for influenceing cell
Type, change the protein expression mass spectrum of cell itself excretion body, can also influence the size of cardiac muscle cell's formation, quantity and extracellular base
The expression of matter.Therefore scholar gradually begins attempt to using the serum for eliminating excretion body to avoid possible external source from influenceing.
The minimizing technology of excretion body at present, it is heavy can be mainly summarized as supercentrifugation, PEG (polyethyleneglycol)
Shallow lake method, immunomagnetic beads method and ultrafiltration.
Wherein, supercentrifugation is method most widely used at present, but supercentrifugation has shortcomings, such as is operated
Time-consuming, removal efficiency low (clearance is about 80%), the specific ultracentrifuge of needs, and parallel processing sample number has
Limit.The PEG precipitation method are mainly used in scientific research field, due to needing to add added ingredient in the sample in extraction process, subsequently
Processing is limited, is not in general suitable for bulk sample and mass disposal.Immunomagnetic beads method is mainly first specific having
The magnetic bead and sample incubation of antibody, combined using the excretion body of antigen-antibody reaction film surface antigen corresponding with having in sample, most
Magnetic bead and excretion body are together adsorbed using magnetic force eventually, realize the removal of excretion body；But the cost of immunomagnetic beads method is higher, one
The secondary a certain specific excretion body subgroup being only capable of in adsorption sample, can not remove whole excretion bodies in culture medium, also not apply to
In the sample of large volume.Part also once carried out excretion body extraction in studying using ultrafiltration, but still needed ultracentrifugation to enter one
Step precipitation excretion body, operating process is tediously long, is not appropriate for Clinical practice.
In summary, the filter that the excretion body that there is no at present can be directed to substantially product sample removes.
The content of the invention
In view of the above mentioned problem and/or other problemses of correlation technique, the utility model provides a kind of removal excretion body
Filter, the filter include：One outer tube, the pipe interior diameter of the outer tube are 8cm~16cm, the outer tube
Axial length be 30cm~50cm；And filtering group, the filtering group includes several hollow filter pipes, described several hollow
Screen pipe is axially parallel arranged in the outer tube along the outer tube, straight in the pipe of each hollow filter pipe
Footpath is 150 μm~250 μm, the pore size filter of tube wall is 2nm~4nm；Space quilt in 60%~80% pipe of the outer tube
The hollow filter pipe occupies；The filtering group has an inlet end portion and an outlet end, the outer tube close to described
The position of the outlet end of filtering group offers an outlet.
Preferably, the inlet end portion of the filtering group and outlet end stretch out in outside the both ends of the outer tube respectively.
Preferably, the filter for removing excretion body also includes setting what is connected with the inlet end portion of the filtering group
Upstream line, the upstream line are provided with the liquid storage container of one-way pneumatic pump and material to be filtered.
Preferably, the filter for removing excretion body also includes the outlet end downstream for being arranged on the filtering group
One collection vessel；The outlet end of the filtering group connects with the collection vessel.
Preferably, it is described remove excretion body filter also include be arranged on the outer tube outlet it is downstream,
For collecting the container of filter material.
Preferably, the hollow filter pipe is the screen pipe of hydrophily polysulphone super-filter membrane.
The filter of the present utility model for removing excretion body, it is simple in construction, larger filter area can be provided, can be entered
Row high-volume sample operations, and may be reused.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the filter for the removal excretion body that the embodiment of the utility model one provides；
Fig. 2 is the schematic cross-sectional view of A-A positions in Fig. 1.
Embodiment
The utility model is further described by the following examples, but to be not limited to these specific for the utility model
Embodiment.
As shown in figure 1, the filter 100 of the removal excretion body of the present embodiment, it mainly includes an outer tube 10 and position
In the filtering group 20 in outer tube 10.
In a specific embodiment of the present utility model, the pipe interior diameter of outer tube 10 is 8cm~16cm, axially
Length is 30cm~50cm.Preferably, in the present embodiment, the pipe interior diameter of outer tube 10 is 12cm, axial length 40cm.
In the present embodiment, outer tube 10 is using hard sleeve made of plastics.
As depicted in figs. 1 and 2, filtering group 20 is provided with outer tube 10, filtering group 20 includes several hollow filter pipes 21.Institute
There is being axially parallel arranged in outer tube 10 along outer tube 10 of hollow filter pipe 21.
In a specific embodiment of the present utility model, the pipe interior diameter of each hollow filter pipe 21 is 150 μm
~250 μm, the pore size filter of tube wall be 2nm~4nm.
In the present embodiment, the pipe interior diameter of hollow filter pipe 21 is 200 μm.
In the present embodiment, the pore size filter of the tube wall of hollow filter pipe 21 is 3nm.
In a specific embodiment of the present utility model, hollow filter pipe 21 is the filtering of hydrophily polysulphone super-filter membrane
Pipe.In the present embodiment, the hydrophily polysulphone super-filter membrane of use (produces purchased from the KOCH hollow fiber ultrafiltration membranes of Coriolis filter membrane company
Product, model V1048-35-PMX), the pore size filter of the filter membrane is 3nm, and the hydrophily polysulphone super-filter membrane is curled into pipe
A diameter of 200 μm of hollow filter pipe 21.
As shown in Fig. 2 most of space in the pipe of outer tube 10 is occupied by several hollow filter pipes 21, hollow filter pipe
A little gaps are also left between 21.In the specific embodiment of the present utility model, 60%~90% in outer tube 10
Space is occupied by hollow filter pipe 21 in pipe.Preferably, in the present embodiment, in about 85% pipe space by hollow filter pipe
21 occupy.
As shown in figure 1, filtering group 20 has an inlet end portion 201 and an outlet end 202.In the present embodiment, it is several
The both ends of hollow filter pipe 21 are all substantially concordant, so as to form overall inlet end portion 201 and outlet end 202.Outer tube 10
The position of close outlet end 202 offer an outlet 101.
Specifically, inlet end portion 201 is the entrance flowed into for material to be filtered.On material to be filtered, referring to needed
Filter off the material except excretion body, such as serum or the culture medium containing serum.
Material to be filtered is further separated into each hollow filter pipe 21 after the inflow of inlet end portion 201.Hollow filter
During the pore size filter of pipe 21 is 3nm, can obstruct the macromolecular that molecular weight is more than 100kDa, therefore excretion body material is blocked on
In empty screen pipe 21, and 21 outer (entrance of filter material (serum or culture medium that eliminate excretion body) outflow hollow filter pipe
Gap in above-mentioned outer tube 10, outside hollow filter pipe 21), the outlet 101 finally opened up from outer tube 10 is flowed out.
In the present embodiment, the inlet end portion 201 of filtering group 20 and outlet end 202 stretch out in the two of outer tube 10 respectively
Outside end.It is hollow that the structure of the stretching outer tube 10 of inlet end portion 201 can ensure that filtered parent material is all introduced into
In screen pipe 21, prevent from entering in the gap in outer tube 10；On the other hand, outlet end 202 stretches out the knot of outer tube 10
Structure can prevent the macromolecular substances such as the excretion body being blocked in hollow filter pipe 21 from entering gap and being mixed into filter
In material.
The filter of the removal excretion body of the present embodiment also includes setting what is connected with the inlet end portion 201 of filtering group 20
Upstream line 30, upstream line 30 are provided with the liquid storage container 50 of one-way pneumatic pump 40 and material to be filtered.When the present embodiment
When filter is started working, one-way pneumatic pump 40 is opened, by the material to be filtered in liquid storage container 40 continuously from upper
Play pipe road 30 is sent to the inlet end portion 201 of filtering group 20, and flow further downstream.For example, in the present embodiment, liquid storage container 50
In 3L materials to be filtered (pending serum) are housed, open one-way pneumatic pump 40, by the extruding of one-way pneumatic pump 40, with
50ml/min speed is injected into serum in each hollow filter pipe 21, while using the pressure of one-way pneumatic pump 50, makes
Obtain serum and flow to outlet end 202 from the inlet end portion 201 of filtering group 20.
In the present embodiment, as shown in figure 1, in upstream line 30, the position of the inlet end portion 201 of close filtering group 20
Provided with a switch valve 301.
As shown in figure 1, the filter of the present embodiment also includes being arranged on the one of the downstream of outlet end 202 of filtering group 20
Collection vessel 60, the outlet end 202 of filtering group 20 connect with collection vessel 60.So as to be blocked in hollow filter pipe 21
The material such as excretion body pass through outlet end 202 and flow into collection vessel 60.In the present embodiment, in the outlet end of filtering group 20
A switch valve 601 is provided between 202 and collection vessel 60.
As shown in figure 1, the filter of the present embodiment also includes downstream, the use for the outlet 101 for being arranged on outer tube 10
In the container 70 for collecting filter material.The hollow filter pipe of filter material (serum or culture medium that eliminate excretion body) outflow
21 outer (entering in above-mentioned outer tube 10, the gap outside hollow filter pipe 21), what is finally opened up from outer tube 10 goes out
Mouth 101 flows into containers 70.In the present embodiment, switch valve 701 is provided between the outlet 101 of outer tube 10 and container 70.
Using such structure, substantial amounts of filter area can be provided, the operation of high-volume sample can be carried out, furthermore,
The hydrophilic surface of hydrophily polysulphone super-filter membrane can accelerate solution by that but excretion body will not be allowed (to there is duplicature knot
Structure) during filtering by strong adsorption hollow filter pipe 21 inner surface, therefore, subsequently can use PBS to hollow
Screen pipe 21 is cleaned, and will be blocked on after the wash-off of the interior excretion body of hollow filter pipe 21, the filter of the present embodiment can
To reuse.
In simple terms, the cleaning process of the filter of the present embodiment is as follows：Liquid storage container is changed into equipped with PBS liquid
Container, open one-way pneumatic pump 40, by the extruding of one-way pneumatic pump 40, PBS liquid noted with 50ml/min speed
Enter into each hollow filter pipe 21, while utilize the pressure of one-way pneumatic pump 50 so that PBS liquid is from filtering group 20
Inlet end portion 201 flows to outlet end 202 (washing out the excretion body and large particulate matter that remain in hollow filter pipe 21),
The downstream of outlet end 202 of filtering group 20 connects the collection vessel 60 for collecting eluate, while also in the outlet 101 of outer tube 10
Downstream connect collect eluate container 70.After cleaning, the filter of the present embodiment is reusable, carries out next time
Filtering.
It should be appreciated that although the present specification is described in terms of embodiments, not each embodiment only includes one
Individual independent technical scheme, this narrating mode of specification is only that those skilled in the art will should say for clarity
For bright book as an entirety, the technical scheme in each embodiment may also be suitably combined to form those skilled in the art can
With the other embodiment of understanding.
The a series of tool described in detail only for feasibility embodiment of the present utility model of those listed above
Body illustrates that they are simultaneously not used to limit the scope of protection of the utility model, all to be made without departing from the utility model skill spirit
Equivalent implementations or change should be included within the scope of protection of the utility model.
Claims (6)
- A kind of 1. filter for removing excretion body, it is characterised in that：The filter includes：One outer tube, the pipe interior diameter of the outer tube are 8cm~16cm, the axial length of the outer tube for 30cm~ 50cm；AndFiltering group, the filtering group include several hollow filter pipes, axial direction of the several hollow filter pipes along the outer tube Abreast it is arranged in the outer tube, the pipe interior diameter of each hollow filter pipe is 150 μm~250 μm, tube wall Pore size filter is 2nm~4nm；Space is occupied by the hollow filter pipe in 60%~80% pipe of the outer tube；The filtering group has an inlet end portion and an outlet end,The position of the outlet end of the close filtering group of the outer tube offers an outlet.
- 2. the filter of excretion body is removed as claimed in claim 1, it is characterised in that：The inlet end portion of the filtering group and Outlet end stretches out in outside the both ends of the outer tube respectively.
- 3. the filter of excretion body is removed as claimed in claim 2, it is characterised in that：The filtering dress for removing excretion body Putting also includes setting the upstream line connected with the inlet end portion of the filtering group, and the upstream line is provided with one-way pneumatic pump With the liquid storage container of material to be filtered.
- 4. the filter of excretion body is removed as claimed in claim 2, it is characterised in that：The filtering dress for removing excretion body Putting also includes the collection vessel for being arranged on the outlet end downstream of the filtering group；The outlet end of the filtering group with it is described Collection vessel connects.
- 5. the filter of excretion body is removed as claimed in claim 2, it is characterised in that：The filtering dress for removing excretion body Putting also includes downstream, for collecting filter material the container for the outlet for being arranged on the outer tube.
- 6. the filter of the removal excretion body as described in any one in claim 1 to 5, it is characterised in that：It is described hollow Screen pipe is the screen pipe of hydrophily polysulphone super-filter membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720465148.1U CN206736237U (en) | 2017-04-28 | 2017-04-28 | A kind of filter for removing excretion body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720465148.1U CN206736237U (en) | 2017-04-28 | 2017-04-28 | A kind of filter for removing excretion body |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206736237U true CN206736237U (en) | 2017-12-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720465148.1U Expired - Fee Related CN206736237U (en) | 2017-04-28 | 2017-04-28 | A kind of filter for removing excretion body |
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| Country | Link |
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| CN (1) | CN206736237U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108085245A (en) * | 2018-02-11 | 2018-05-29 | 中南大学 | A kind of room temperature isolates and purifies the casing filtering device of excretion body |
| TWI808425B (en) * | 2021-05-28 | 2023-07-11 | 財團法人工業技術研究院 | Cell purification module, cell purification system and operation method thereof |
-
2017
- 2017-04-28 CN CN201720465148.1U patent/CN206736237U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108085245A (en) * | 2018-02-11 | 2018-05-29 | 中南大学 | A kind of room temperature isolates and purifies the casing filtering device of excretion body |
| TWI808425B (en) * | 2021-05-28 | 2023-07-11 | 財團法人工業技術研究院 | Cell purification module, cell purification system and operation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171212 |