CN215609757U

CN215609757U - Exosome separation and purification device

Info

Publication number: CN215609757U
Application number: CN202122230203.8U
Authority: CN
Inventors: 李阿峰; 明磊国; 王清霞
Original assignee: Shaanxi Kemei Zhishang Biotechnology Co ltd
Current assignee: Shaanxi Kemei Zhishang Biotechnology Co ltd
Priority date: 2021-09-15
Filing date: 2021-09-15
Publication date: 2022-01-25
Anticipated expiration: 2031-09-15

Abstract

The utility model relates to an exosome separating and purifying device which comprises a cylinder, a peristaltic pump, a shunt cover, a first filter layer, a second filter layer, a third filter layer, a steel plate and a collecting tank, wherein the shunt cover is provided with a peristaltic pump interface and a shunt passage, the peristaltic pump is connected with the peristaltic pump interface, the shunt cover is fixed on the cylinder, the first filter layer, the second filter layer and the third filter layer are sequentially arranged in the cylinder from top to bottom, and the collecting tank is arranged at the bottom of the cylinder; the method solves the problems that in the prior art, the exosome is extracted and separated only by methods such as ultracentrifugation, immunomagnetic beads, ultrafiltration, precipitation or a kit and the like in a laboratory, and is only limited by experimental dosage and difficult to industrialize.

Description

Exosome separation and purification device

Technical Field

The utility model relates to the technical field of exosome extraction, in particular to an exosome separation and purification device.

Background

The exosome carries a large amount of specific proteins and functional bioactive substances such as mRNAs and miRNAs, participates in physiological processes such as cell communication, cell migration, angiogenesis promotion, anti-tumor immunity and the like in vivo, and is closely related to the occurrence and the process of various diseases; due to the special structure and function of the exosome, the exosome has potential application value, can be used as a biological index for diagnosing various diseases on one hand, and can be used as a treatment means on the other hand, and can be possibly used as a natural carrier of a medicament for clinical treatment in the future; the separation and purification of exosomes are always the concerns of researchers, and the obtaining of high-purity exosomes is crucial to the subsequent research; at present, the extraction and separation of exosomes are realized only in a laboratory by methods such as ultracentrifugation, immunomagnetic beads, ultrafiltration, precipitation or a kit, and the like, and the method is limited only by experimental dosage and is difficult to industrialize.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide an exosome separation and purification device aiming at the defects of the prior art, and solves the problems that the prior art only adopts ultracentrifugation, immunomagnetic beads, ultrafiltration, precipitation or a kit and other methods in a laboratory to realize the extraction and separation of exosomes, is only limited by experimental dosage and is difficult to industrialize.

The utility model is realized by adopting the following technical scheme:

the utility model provides an exosome separation and purification device, includes barrel, peristaltic pump, reposition of redundant personnel lid, first filter layer, second filter layer, third filter layer, steel sheet and collecting vat, the reposition of redundant personnel lid is equipped with peristaltic pump interface and subchannel, the peristaltic pump with peristaltic pump interface connection, the reposition of redundant personnel lid is fixed in the barrel, from the top down is equipped with in proper order in the barrel first filter layer the second filter layer with the third filter layer, the collecting vat set up in the barrel bottom.

Preferably: the steel plate is arranged between the first filter layer and the second filter layer, the steel plate is arranged between the second filter layer and the third filter layer, and the steel plate is concave.

Preferably: the peristaltic pump is characterized by further comprising a safety valve, and the safety valve is externally connected to the peristaltic pump interface.

Preferably: the filter comprises a cylinder body, and is characterized by further comprising a valve, wherein the valve is arranged outside the cylinder body between the second filter layer and the third filter layer.

Preferably: the first filter layer comprises a first filter membrane, and the filtration pore size of the first filter membrane is 500nm-300 nm.

Preferably: the second filter layer comprises a second filter membrane, and the filtration pore size of the second filter membrane is 300nm-150 nm.

Preferably: the third filter layer comprises a third filter membrane, and the filtration pore size of the third filter membrane is 30nm-3 nm.

Compared with the prior art, the utility model has the following beneficial technical effects:

the peristaltic pump is used as a power source, and is used for supplying high pressure to liquid and pressing the liquid into a separation and purification device; the top end of the device is provided with a shunt cover, the upper end of the device is provided with a peristaltic pump connecting interface, the interface is an inflow channel, the cover is provided with a shunt channel structure for increasing turbulence, and a plurality of channels play a role in increasing turbulence in the device and inhibit filtrate from forming a gel layer on a filtering membrane layer so as to reduce the filtering speed of the device; the first layer is a detachable filtering membrane arranged below the cover and mainly used for filtering impurities in the liquid; a stainless steel plate with a turbulent flow structure is arranged below the filter membrane; the second layer of filter membrane is arranged below the first layer of filter membrane, the impurities of the protein with the aperture larger than that of the exosome are mainly separated, and a stainless steel plate with a turbulent flow structure is arranged below the filter membrane; the third layer of filter membrane is mainly purified exosome, inorganic salt, micromolecular protein and the like with the particle size of less than 30nm are filtered, and the bottom layer is a filter liquid collecting part.

Drawings

The utility model is further described with the following figures:

FIG. 1 is a schematic structural diagram of an exosome separation and purification device of the present invention.

Description of the reference numerals

1. A barrel; 2. a shunt cover; 3. a first filter layer; 4. a second filter layer; 5. a third filter layer; 6. a steel plate; 7. collecting tank; 8. a safety valve; 9. a valve; 10. and a branch channel.

Detailed Description

In order that the above objects, features and advantages of the present invention can be more clearly understood, a detailed description of the present invention will be given below with reference to the accompanying drawings and specific embodiments. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict. In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," and "connected" are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The first embodiment is as follows:

as shown in fig. 1: an exosome separating and purifying device comprises a cylinder body 1, a peristaltic pump, a shunt cover 2, a first filter layer 3, a second filter layer 4, a third filter layer 5, a steel plate 6 and a collecting tank 7, wherein the peristaltic pump is used as a power source for supplying high pressure to liquid and is pressed into the separating and purifying device; the top end of the cylinder body 1 is provided with the shunt cover 2, the upper end of the shunt cover 2 is provided with a peristaltic pump connecting interface which is an inflow channel, the shunt cover 2 is provided with a shunt channel 10 structure for increasing turbulence, and a plurality of channels play a role in increasing turbulence in the device and inhibit filtrate from forming a gel layer on a filtering membrane layer so as to reduce the filtering speed of the device; the first filter layer 3, the second filter layer 4 and the third filter layer 5 are fixed in the cylinder body 1 sequentially through bolts from top to bottom, the first filter layer 3, the second filter layer 4 and the third filter layer 5 can be detached, the first filter layer 3 comprises a first filter membrane, the filter aperture of the first filter membrane is 500nm-300nm, and impurities in liquid are mainly filtered; the second filter layer 4 comprises a second filter membrane, the filtration pore diameter of the second filter membrane is 300nm-150nm, and impurities of the protein with the pore diameter larger than that of the exosome are mainly separated; the third filter layer 5 comprises a third filter membrane, the filtration pore diameter of the third filter membrane is 30nm-3nm, and inorganic salt and micromolecular protein which are smaller than 30nm are filtered; the steel plate 6 is arranged between the first filter layer 3 and the second filter layer 4, and the steel plate 6 is used as a substrate of the filter membrane; because the filter membrane is a fiber product or a porous anodic aluminum membrane, the filter membrane cannot be supported without a substrate and cannot bear the pressure of a peristaltic pump, in addition, the steel plate 6 is also used as a bearing part for increasing turbulence, the steel plate 6 is arranged between the second filter layer 4 and the third filter layer 5, the steel plate 6 is concave, and the collecting tank 7 is arranged at the bottom of the cylinder 1; still include relief valve 8, peristaltic pump interface connection relief valve 8, after atmospheric pressure reached certain pressure, 8 valves of relief valve can be opened automatically and arrange the pressure, still include valve 9 and still include the valve, the second filter layer with between the third filter layer be equipped with outside the barrel the valve, the exosome passes through the valve is collected, the collecting vat is collected the waste liquid.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

The above-mentioned embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the same; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. An exosome separation and purification device, characterized in that: including barrel, peristaltic pump, reposition of redundant personnel lid, first filter layer, second filter layer, third filter layer, steel sheet and collecting vat, the reposition of redundant personnel lid is equipped with peristaltic pump interface and subchannel, the peristaltic pump with peristaltic pump interface connection, the reposition of redundant personnel lid is fixed in the barrel, from the top down is equipped with in proper order in the barrel first filter layer the second filter layer with the third filter layer, the collecting vat set up in the barrel bottom.

2. Exosome separating and purifying device according to claim 1, characterized in that: the steel plate is arranged between the first filter layer and the second filter layer, the steel plate is arranged between the second filter layer and the third filter layer, and the steel plate is concave.

3. Exosome separating and purifying device according to claim 1, characterized in that: the peristaltic pump is characterized by further comprising a safety valve, and the safety valve is externally connected to the peristaltic pump interface.

4. Exosome separating and purifying device according to claim 1, characterized in that: the filter comprises a cylinder body, and is characterized by further comprising a valve, wherein the valve is arranged outside the cylinder body between the second filter layer and the third filter layer.

5. Exosome separating and purifying device according to claim 1, characterized in that: the first filter layer comprises a first filter membrane, and the filtration pore size of the first filter membrane is 500nm-300 nm.

6. Exosome separating and purifying device according to claim 1, characterized in that: the second filter layer comprises a second filter membrane, and the filtration pore size of the second filter membrane is 300nm-150 nm.

7. Exosome separating and purifying device according to claim 1, characterized in that: the third filter layer comprises a third filter membrane, and the filtration pore size of the third filter membrane is 30nm-3 nm.