CN213375482U - Filter equipment is used in protein purification separation - Google Patents
Filter equipment is used in protein purification separation Download PDFInfo
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- CN213375482U CN213375482U CN202020229514.5U CN202020229514U CN213375482U CN 213375482 U CN213375482 U CN 213375482U CN 202020229514 U CN202020229514 U CN 202020229514U CN 213375482 U CN213375482 U CN 213375482U
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- filtering
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- ice
- box body
- protein purification
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Abstract
The utility model discloses a filtering device for protein purification and separation, which comprises a first support frame, an ice supplementing mechanism and a filtering mechanism, wherein the ice supplementing mechanism is arranged at the top of the first support frame, and the filtering mechanism is arranged below the ice supplementing mechanism; the filtering mechanism comprises a box body, a filtering box is arranged in the box body, the filtering box is arranged above the bottom of the box body through a second supporting frame, pipelines are arranged on two sides of the ice supplementing mechanism and lead to an ice isolating layer between the box body and the filtering box, a temperature sensor connected with the ice supplementing mechanism is arranged on the inner wall of one side of the box body, a funnel used for filtering is arranged in the center of the top of the filtering box, and a collecting box is arranged below the funnel in the filtering box; the collecting box top is a plurality of apertures of evenly distributed and is used for carrying out the secondary filter's filter screen to solution, the collecting box is inside to be equipped with the U type and is used for collecting the protein solution after filtering and can dismantle the collection container of being connected with the collecting box, can guarantee the activity of protein, and the practicality is strong.
Description
Technical Field
The utility model relates to a filter equipment technical field specifically is a filter equipment is used in protein purification separation.
Background
Proteins play a very important role in the course of the life activities of cells and organisms. The prerequisite for studying the function of proteins is the ability to purify the protein in a pure phase. The separation and purification of proteins is widely used in biological research applications, and is an important operation technology. Protein purification is carried out by a method of pre-treating and then grading, generally, cell walls are firstly crushed, then different proteins are separated and filtered, and finally target proteins are obtained. In protein purification experiments, ascites or serum needs to be simply filtered to remove insoluble impurities, and a low temperature environment needs to be maintained in the process to maintain the activity of the protein.
The utility model discloses the device of patent number CN201520335156.5 provides a simple structure, low price, safe efficient filter equipment for protein separation purification, though do not need during the filtration that operating personnel held the funnel, also can carry out secondary filter to the sample, but whole device is gone on under the normal atmospheric temperature condition, can not make protein keep its original activity, can destroy the inner structure of protein even.
In view of the above problems, an improved filtration apparatus for protein purification and separation has been devised.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a protein purification is filter equipment for separation to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: a filtering device for protein purification and separation comprises a first support frame, an ice supplementing mechanism and a filtering mechanism, wherein the ice supplementing mechanism is arranged at the top of the first support frame, and the filtering mechanism is arranged below the ice supplementing mechanism; the filtering mechanism comprises a box body, a filtering box is arranged in the box body, the filtering box is arranged above the bottom of the box body through a second supporting frame, pipelines are arranged on two sides of the ice supplementing mechanism and lead to an ice isolating layer between the box body and the filtering box, a temperature sensor connected with the ice supplementing mechanism is arranged on the inner wall of one side of the box body, a funnel used for filtering is arranged in the center of the top of the filtering box, and a collecting box is arranged below the funnel in the filtering box; the collecting box top is a filter screen that a plurality of apertures of evenly distributed are used for carrying out secondary filter to solution, collecting box inside is equipped with the U type and is used for collecting the protein solution after filtering and can dismantle the collection container of being connected with the collecting box.
Preferably, the ice supplementing mechanism is connected with a manual switch capable of placing ice into the ice isolation layer.
Preferably, the bottom of the box body is provided with a valve for draining water.
Preferably, the funnel is in an inverted cone shape.
Preferably, a drainage tube is arranged below the funnel, and the drainage tube is made of a material which does not adsorb protein.
Preferably, the filter box is internally provided with a sterilization adsorbent for sterilizing the solution.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the ice supplementing mechanism in the device is connected with the manual switch which can release ice into the ice isolation layer, when the temperature rise of the temperature sensor is observed from the external controller to influence the activity of the protein, the ice is added into the ice isolation layer through the manual switch, the temperature is effectively reduced, and the activity of the protein is kept; the bottom of the box body is provided with a valve, so that water in the ice isolation layer can be conveniently discharged.
2. The funnel in the device is in the shape of an inverted cone, so that the inflow of a sample to be filtered can be increased, and the filtering efficiency is improved; the drainage tube below the funnel is made of a material which does not adsorb protein, so that a sample cannot be adsorbed on the inner wall of the drainage tube in the drainage process; the sterilizing adsorbent for sterilizing the solution is arranged in the filter box, so that bacteria are prevented from entering the collecting box, bacterial infection is prevented, and the protein purity is reduced; whole device can carry out the secondary to solution and filter, can improve filtration efficiency, and final sample is more stable, even, and the ice supplementing mechanism can reduce the probability that the albumen structure changed, simple structure, convenient operation.
Drawings
FIG. 1 is a schematic view of a structure of a filtration apparatus for protein purification and separation.
FIG. 2 is a schematic view showing the structure of a collection tank of a filtering apparatus for protein purification and separation.
FIG. 3 is a schematic view showing the structure of a funnel in a filtering apparatus for protein purification and separation.
Wherein: 1-first support frame, 2-mend ice mechanism, 3-ice separation layer, 4-valves, 5-rose box, 6-funnel, 7-temperature sensor, 8-box, 9-second support frame, 10-collecting box, 12-filter screen, 13-collection container, 14-manual switch.
Detailed Description
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, in an embodiment of the present invention, a filtering apparatus for protein purification and separation includes a first support frame 1, an ice supplement mechanism 2 and a filtering mechanism, wherein the ice supplement mechanism 2 is disposed on the top of the first support frame, and the filtering mechanism is disposed below the ice supplement mechanism 2;
the filtering mechanism comprises a box body 8, a filtering box 5 is arranged in the box body 8, the filtering box 5 is arranged above the bottom of the box body 8 through a second supporting frame 9, pipelines are arranged on two sides of the ice supplementing mechanism 2 and lead to an ice isolation layer 3 between the box body 8 and the filtering box 5, a temperature sensor 7 connected with an external controller is arranged on the inner wall of one side of the box body 8, a funnel 6 for filtering is arranged in the center of the top of the filtering box 5, and a collecting box 10 is arranged below the funnel 6 in the filtering box 5;
the top of the collecting box 10 is provided with a filter screen 12 which is uniformly distributed with a plurality of small holes and is used for carrying out secondary filtration on the solution, and a U-shaped collecting container 13 which is used for collecting the filtered protein solution and is detachably connected with the collecting box 10 is arranged inside the collecting box 10.
The ice supplementing mechanism 2 is connected with a manual switch 14 capable of releasing ice into the ice isolation layer 3, and when the temperature rise of the temperature sensor is observed from an external controller to influence the activity of the protein, the ice is added into the ice isolation layer through the manual switch, so that the temperature is effectively reduced, and the activity of the protein is kept.
The bottom of the box body 8 is provided with a valve 4 for draining water, and water in the ice isolation layer is conveniently drained after ice melts.
The funnel below is equipped with the drainage tube, the drainage tube is for not adsorbing protein class material, can not lead to the sample to adsorb on the drainage tube inner wall in the drainage process, reduces extravagantly.
The sterilizing adsorbent used for sterilizing the solution is arranged in the filter box 5, so that bacteria are prevented from entering the collecting box, bacterial infection is prevented, and the protein purity is reduced.
The utility model discloses a theory of operation is: put ice in to the ice isolation layer through manual switch, reduce the temperature in the rose box, keep the activity of protein, put into the filter membrane in the funnel, will wait to filter solution and pour into the funnel, solution filters the back through the filter membrane, on the filter screen at collection box top is collected in the drainage tube drainage, the filter screen can carry out the secondary filter to solution, solution after the secondary filter can flow in and collect the container, accomplish the filtration and the collection of sample, the stability and the even of the protein structure of final sample have been guaranteed, moreover, the steam generator is simple in structure, high in operation convenience and strong in practicability.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (6)
1. A filtering device for protein purification and separation comprises a first support frame (1), an ice supplementing mechanism (2) and a filtering mechanism, and is characterized in that the ice supplementing mechanism (2) is arranged at the top of the first support frame, and the filtering mechanism is arranged below the ice supplementing mechanism (2);
the filtering mechanism comprises a box body (8), a filtering box (5) is arranged in the box body (8), the filtering box (5) is arranged above the bottom of the box body (8) through a second supporting frame (9), ice isolating layers (3) leading to the box body (8) and the filtering box (5) through pipelines are arranged on two sides of the ice supplementing mechanism (2), a temperature sensor (7) connected with an external controller is arranged on the inner wall of one side of the box body (8), a funnel (6) used for filtering is arranged in the center of the top of the filtering box (5), and a collecting box (10) is arranged below the funnel (6) in the filtering box (5);
the top of the collecting box (10) is provided with a filter screen (12) which is uniformly distributed with a plurality of small holes and is used for carrying out secondary filtration on the solution, and a U-shaped collecting container (13) which is used for collecting the filtered protein solution and is detachably connected with the collecting box (10) is arranged inside the collecting box (10).
2. The filtering device for protein purification and separation as claimed in claim 1, wherein the ice replenishing mechanism (2) is connected with a manual switch (14) capable of discharging ice into the ice separating layer (3).
3. The filtering device for protein purification and separation according to claim 1, wherein a valve (4) for draining water is provided at the bottom of the tank (8).
4. The filtering device for protein purification and separation as claimed in claim 1, wherein said funnel (6) is in the shape of an inverted cone.
5. The filtering device for protein purification and separation according to claim 1, wherein a drainage tube is arranged below the funnel (6), and the drainage tube is made of a material which does not adsorb proteins.
6. A filtering apparatus for protein purification and separation according to claim 1, wherein a sterilizing adsorbent for sterilizing the solution is provided inside the filtering tank (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020229514.5U CN213375482U (en) | 2020-02-29 | 2020-02-29 | Filter equipment is used in protein purification separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020229514.5U CN213375482U (en) | 2020-02-29 | 2020-02-29 | Filter equipment is used in protein purification separation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213375482U true CN213375482U (en) | 2021-06-08 |
Family
ID=76176941
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020229514.5U Expired - Fee Related CN213375482U (en) | 2020-02-29 | 2020-02-29 | Filter equipment is used in protein purification separation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213375482U (en) |
-
2020
- 2020-02-29 CN CN202020229514.5U patent/CN213375482U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210608 |
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| CF01 | Termination of patent right due to non-payment of annual fee |