CN203878152U - Magnetic frame for separating biological samples - Google Patents
Magnetic frame for separating biological samples Download PDFInfo
- Publication number
- CN203878152U CN203878152U CN201420160909.9U CN201420160909U CN203878152U CN 203878152 U CN203878152 U CN 203878152U CN 201420160909 U CN201420160909 U CN 201420160909U CN 203878152 U CN203878152 U CN 203878152U
- Authority
- CN
- China
- Prior art keywords
- magnetic frame
- magnetic
- separation vessel
- magnet base
- magnetic part
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000005291 magnetic effect Effects 0.000 title claims abstract description 111
- 239000012472 biological sample Substances 0.000 title claims abstract description 18
- 238000000926 separation method Methods 0.000 claims abstract description 57
- 238000000034 method Methods 0.000 abstract description 19
- 238000000605 extraction Methods 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 description 11
- 239000000523 sample Substances 0.000 description 9
- 239000011324 bead Substances 0.000 description 6
- 239000003292 glue Substances 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 239000012634 fragment Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000007885 magnetic separation Methods 0.000 description 3
- 108010067770 Endopeptidase K Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 239000012445 acidic reagent Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 229910052779 Neodymium Inorganic materials 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- ZGDWHDKHJKZZIQ-UHFFFAOYSA-N cobalt nickel Chemical compound [Co].[Ni].[Ni].[Ni] ZGDWHDKHJKZZIQ-UHFFFAOYSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- QEFYFXOXNSNQGX-UHFFFAOYSA-N neodymium atom Chemical compound [Nd] QEFYFXOXNSNQGX-UHFFFAOYSA-N 0.000 description 1
- 239000012716 precipitator Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
This utility model provides a magnetic frame for separating biological samples. The magnetic frame comprises a bracket and a magnetometric base, wherein the magnetometric base is placed on the bracket, a locking device is mounted on the bracket, and the locking device is used for locking a separation container on the magnetic frame. When the separation container in which biological samples are filled is placed on the magnetometric base, the separation container can be locked on the magnetic frame by virtue of the locking device. During biological samples are extracted, if the separation container is turned over, the separation container can not fall from the magnetic frame, and thus the operation process is very convenient and the magnetic frame is suitable for the extraction of biological samples.
Description
Technical field
The utility model relates to biological sample separation field, relates in particular to a kind of magnetic separation rack separating for biological sample.
Background technology
Magnetic separation technique has a wide range of applications in the separating of cytobiology, immunology, diagnostics and pharmacy etc., and can realize the object of the materials such as fast separating and purifying cell, albumen or nucleic acid.As compared with the precipitator method, centrifuging, ion exchange column method, magnetic separation technique has that separating device is few, velocity of separation fast, extraction efficiency high with conventional partition method.
Magnetic frame is adsorbable magnetic particle in biological magnetic lock out operation, thereby realizes sharp separation and the purifying of target molecule in complex mixture, is a kind of simple in structure, easy to operate magnetic separating apparatus.When use, will the separation vessel of biological sample be housed, for example 96 orifice plates, put into magnetic frame.Magnetic substance in sample will be enriched on the magneticsubstance in separation agent, and is fitted on the tube wall of separation vessel.Remove not by the material of magnetic absorption, and through obtaining the biological sample of purifying after repeatable operation repeatedly.But existing magnetic frame is only used for support separation vessel, the separation vessel of putting thereon can not be fixed.In extraction from biological material process, if upset separation vessel, existing magnetic frame just can not complete.Because in the time that magnetic frame overturns, separation vessel can drop from magnetic frame, so existing magnetic frame can not adapt to more lock out operation.
Utility model content
For overcoming the deficiencies in the prior art, the purpose of this utility model is to provide a kind of magnetic frame for separating of biological sample, comprises support, is placed on the magnet base on support, locking latches is installed on support, and described locking latches is locked at separation vessel on magnetic frame.
Further, described locking latches comprises clip and baffle plate assembly, and clip is arranged on one end of locking latches, and baffle plate assembly is arranged on the other end of locking latches.
Further, described baffle plate assembly comprises push pedal, screw and cutting die.
Further, in described magnet base, magnetic part is installed.
Further, described magnetic part is convex shape.
Further, in described magnet base, be provided with magnetic part holding tank.
Further, described magnetic part holding tank is convex shape cavity.
Further, on described magnet base, also comprise support hole.
The beneficial effects of the utility model are: magnetic frame described in the utility model can be fixed the separation vessel of putting thereon, in extraction from biological material process, if upset separation vessel, separation vessel just can not drop from magnetic frame, therefore operating easily of biological extraction.Locking latches described in the utility model makes the placement of 96 orifice plates and is fixed in a step to complete simultaneously, and does not need to increase extra fixing step.In the time that magnetic part adopts convex shape structure, the combination between magnetic part and magnet base just does not need to fix with glue.In the time that magnetic part is impaired, can completes more and change jobs easily like this.Design described in the utility model has increased the contact area of magnet and separation vessel, increases the area of magnetic absorption, has improved the separation efficiency of biological sample.
Brief description of the drawings
Fig. 1 is magnetic frame structural representation.
Fig. 2 is the magnetic frame structural representation that separation vessel is housed.
Fig. 3 is the first location schematic diagram of separation vessel at elastic piece structure locking latches.
Fig. 4 is the second position schematic diagram of separation vessel at elastic piece structure locking latches.
Fig. 5 is three position view of separation vessel at elastic piece structure locking latches.
Fig. 6 is convex shape magnetic part holding tank structural representation.
Fig. 7 is the magnetic part holding tank structural representation of tool ramp structure.
Embodiment
Below in conjunction with concrete accompanying drawing, the utility model is described in detail.These specific embodiments are only limited the enumerating under the utility model spirit, do not get rid of one of ordinary skill in the art prior art and the utility model in conjunction with and other specific embodiments of producing.
As illustrated in fig. 1 and 2, magnetic frame 100 for separating of biological sample comprises support 1, and magnet base 2 is placed on support 1, comprises a locking latches on support, in the time the separation vessel 200 of biological sample is housed is placed on magnet base, locking latches can be locked at separation vessel 200 on magnetic frame.Magnetic part is installed on magnet base.
In one embodiment, magnetic frame locking latches comprises clip and baffle plate assembly.A clip 4 is installed in one end of locking latches, and the other end is equipped with a baffle plate assembly.After separation vessel 200 is placed on magnet base 2, the one end of first clip 4 being clamped to separation vessel, is then resisted against baffle plate assembly the other end of separation vessel, thereby separation vessel is fixed on magnetic frame.Due to some separation vessel, for example standard template also has bottom plate eaves (the similar the brim of a hat), and clip 4 and baffle plate assembly can also reach by blocking plate eaves the object of retaining plate.Described baffle plate assembly comprises a push pedal 6, screw 7 and cutting die 8.In the time that screw 7 screws inward, promote push pedal 6 and rely on separation vessel, thereby coordinate separation vessel is fixed on magnetic frame tightly with clip.The separation vessel being locked just can not fall down from magnetic frame, and the operation of being inverted like this separation vessel just can complete.
In another embodiment as in Figure 3-5, magnetic frame locking latches is shell fragment 9 and the shell fragment support 10 that is arranged on magnetic frame two ends, and shell fragment is arranged on shell fragment support, and shell fragment comprises elastic arm 11 and shell foot 12.Angle between elastic arm 11 and shell foot 12 is greater than 90 degree.Shell foot bottom surface 13 does not touch the upper surface of magnet base, and the distance between shell foot bottom surface 13 and magnet base upper surface is just in time the height of separation vessel.In the time that separation vessel 200 is put into magnetic frame along shell foot, the wooden partition of separation vessel 200 has applied an outside power to shell foot, and extruded shell foot opens to both sides, makes separation vessel descending smoothly.When separation vessel is during lower than shell foot, shell foot returns to home position after losing outside thrust, and shell foot bottom surface just in time mortgages on separation vessel and compressed separation vessel, thereby separation vessel is fixed on magnetic frame.While needing to take out separation vessel after lock out operation completes, apply a power shell foot is no longer resisted against on separation vessel to shell foot, separation vessel 200 just can be taken away from magnetic frame smoothly like this.The design of the present embodiment makes the placement of separation vessel and is fixed in a step to complete simultaneously, and does not need multiple other fixing step.
In an embodiment as shown in Fig. 1 and Fig. 6, be provided with magnetic part holding tank 15 in magnet base 2, magnetic holding tank is parallel each other.Magnetic part leaves in this holding tank, and magnetic part can long strip shape or circle.In one embodiment, magnetic part is to be bonded in holding tank with glue.Embodiment as shown in Figure 6, holding tank 15 is a convex shape cavity, and magnetic part 30 is a convex shape, and the convex shape of magnetic part is less than the convex shape of holding tank.Two shoulders 31 of magnetic part are just in time resisted against the internal surface 5 of magnet base 2, and magnetic part is in without the hydropexic situation of glue like this, and magnetic part can be from the upper shed of holding tank 16 through skidding off.In the time that whole magnet base 2 is installed on support, magnetic part just can not come off.Because this mode is not used glue, so in the time changing the magnetic part damaging, directly the magnetic part damaging is taken out from magnet base, and do not need to remove arduously glue.Magnetic part is installed also very convenient, as long as the shoulder of magnetic part 31 is pushed in the cavity of holding tank.In order further to strengthen stability and the stability of magnetic part, can shoulder 31 be fixedly connected with magnet base 2 by modes such as screws.
In a preferred scheme, the magnetic part of convex shape is not that tool is magnetic all over, and it comprises base plate 33 and magnet 32, and base plate 33 is non-magnetic substances, and magnet 32 is fixed on base plate 33.Magnet 32 is through holding tank 15, and two shoulders 31 of base plate are just in time resisted against the internal surface 5 of magnet base 2, and magnet just can not skid off from holding tank.Magnet base 2 can be bonded on support 1 with glue etc., and in a preferred scheme, magnet base is to be fixed on support with mounting block 30, for example, use screw means on support, and convenient so i.e. dismounting is conducive to again change magnetic part.
In one embodiment, the opening surface of the magnetic part holding tank on magnet base is not parallel with magnet base bottom surface, but angled, and it is also angled with magnet base bottom surface to be placed on magnetic part surface in holding tank, and such design just increases the area for magnetic absorption.In the mode shown in Fig. 7, the magnet contact surface 35 that magnetic part contacts with separation vessel is rendered as inclined-plane, and the bottom outer wall 210 of inclined-plane and separator 200 is just in time fitted, and magnetic part just has more area to touch the outer wall of separation vessel like this.Shown in Fig. 7, on magnet base, the position that each separation vessel is corresponding is provided with two magnetic part holding tanks, and the extended line of two holding tank openings can intersect, and holding tank opening is an inclined-plane like this.After the magnet coordinating with this holding tank is put into wherein, magnet and separator contact surface are an inclined-plane.Such design can also allow the separation vessel being placed on it more firm.
When use, magnet base 2 is fixed on support 1.On magnet base, can also comprise support hole 40, for example, at the bottom of the pipe of the each extraction tube of 96 hole PCR plate, just in time drop in support hole, thereby prevent that 96 hole PCR plates are moved on magnetic frame.
Described biological sample separation vessel is selected from 96 hole PCR plates, enzyme plate, 24 holes, 48 holes, 96 hole depth orifice plates, reservoir etc.
Described magnet is the materials such as neodymium permanent magnet material, electromagnetism, cobalt nickel magnet, can be used for adsorbing magnetic particle in biological magnetic lock out operation, thereby realizes target molecule ground sharp separation and purifying in complex mixture.
Embodiment 1
Separate nucleic acid system in biological sample, comprises magnetic frame 100 described in the utility model and KBM separate nucleic acid reagent.Described separate nucleic acid reagent comprises: damping fluid ME1, rinsing liquid Wash1D, rinsing liquid Wash2A, Proteinase K, magnetic bead suspension B eads C, elution buffer Eluent B and Virahol.
The operation steps of utilizing biological sample separate nucleic acid system described in the utility model to extract nucleic acid comprises:
1. in 96 deep-well plates, add the Proteinase K of 20ul;
2. add sample;
3. in every hole, add 450ul damping fluid ME1, lash to mix or vibrate and mix;
4. 70 DEG C of water-baths, 20 minutes, digesting material;
5. every hole adds 100ul magnetic bead suspension B eads C and 400ul Virahol, lashes to mix or vibrate to mix 5 minutes;
6. 96 deep-well plates are placed on magnetic frame and leave standstill 30 seconds, in the time that magnetic bead adsorbs completely, carefully remove liquid; Or by the fastening on magnetic frame of 96 orifice plates, liquid is abandoned in upset, and can firmly overturn and pat 96 deep-well plates on thieving paper, abandon to the greatest extent raffinate.
7. deep-well plates is taken off from magnetic frame, add 750ul rinsing liquid Wash1D, lash to mix or vibrate and mix;
8. 96 deep-well plates are placed on magnetic frame, 96 orifice plates are left standstill to 30 seconds on magnetic frame, the careful liquid of removing in the time that magnetic bead adsorbs completely; Or by the fastening on magnetic frame of 96 orifice plates, liquid is abandoned in upset, and can firmly overturn and pat 96 orifice plates on thieving paper, abandon to the greatest extent raffinate.
9. repeating step 7-8.
10. deep-well plates is taken off from magnetic frame, add 1000ul rinsing liquid Wash1D, lash to mix or vibrate and mix;
11. are placed on 96 deep-well plates on magnetic frame, and 96 orifice plates are left standstill to 30 seconds on magnetic frame, the careful liquid of removing in the time that magnetic bead adsorbs completely; Or by the fastening on magnetic frame of 96 orifice plates, liquid is abandoned in upset, and can firmly overturn and pat 96 deep-well plates on thieving paper, abandon to the greatest extent raffinate.
12. repeating steps 10 and 11;
13. room temperatures are dried or heat drying 10-15 minute;
14. take off deep-well plates from magnetic frame, add the elution buffer Eluent B of 80-250ul, lash to mix or vibrate to mix 5 minutes;
15. are placed on deep-well plates on magnetic frame and leave standstill 30 seconds, in the time that magnetic bead adsorbs completely, carefully DNA solution are transferred to collecting board, and preserve or complete follow-up test experience in felicity condition.
Result shows:
1. analyze by ultraviolet spectrophotometer, the sample DNA purity that adopts this magnetic frame to extract with reagent is consistent with the sample DNA purity of pellosil method extraction.As shown in table 1.
Table 1 ultraviolet spectrophotometer is analyzed the sample DNA purity that two kinds of methods are extracted
2. analyze by agarose gel electrophoresis and gel imaging instrument, the sample DNA that adopts this magnetic frame and reagent to extract must be measured the sample DNA extracting than pellosil method and must measure higher.M representation DNA molecular weight standard; Column represents pellosil extracting method; KBM represents KBM paramagnetic particle method extracting method.
3. detect analysis by PCR, in the sample DNA that adopts this magnetic frame and reagent to extract, do not contain PCR inhibitor.Column represents pellosil extracting method; KBM represents KBM paramagnetic particle method extracting method; P represents positive sample.
To sum up result shows, the DNA purity of utilizing method described in the utility model to obtain is high, can be directly used in PCR, equimolecular biological experiment is cut, hybridized to enzyme.Be held on magnetic frame in 96 deep-well plates, liquid is abandoned in upset, and can firmly overturn and pat deep-well plates on thieving paper, abandons to the greatest extent in the operating process of raffinate, and deep-well plates can not drop from magnetic frame.Whole operation is highly suitable for manual extraction, simple and convenient.
Claims (7)
1. the magnetic frame of separation of biological samples, comprise support, be placed on the magnet base on support, locking latches is installed on support, described locking latches is locked at separation vessel on magnetic frame, described locking latches comprises clip and baffle plate assembly, and clip is arranged on one end of locking latches, and baffle plate assembly is arranged on the other end of locking latches.
2. magnetic frame according to claim 1, is characterized in that, described baffle plate assembly comprises push pedal, screw and cutting die.
3. magnetic frame according to claim 1, is characterized in that, in described magnet base, magnetic part is installed.
4. magnetic frame according to claim 3, is characterized in that, described magnetic part is convex shape.
5. magnetic frame according to claim 1, is characterized in that, is provided with magnetic part holding tank in described magnet base.
6. magnetic frame according to claim 5, is characterized in that, described magnetic part holding tank is convex shape cavity.
7. magnetic frame according to claim 1, is characterized in that, also comprises support hole on described magnet base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420160909.9U CN203878152U (en) | 2014-04-01 | 2014-04-01 | Magnetic frame for separating biological samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420160909.9U CN203878152U (en) | 2014-04-01 | 2014-04-01 | Magnetic frame for separating biological samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203878152U true CN203878152U (en) | 2014-10-15 |
Family
ID=51678674
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420160909.9U Expired - Lifetime CN203878152U (en) | 2014-04-01 | 2014-04-01 | Magnetic frame for separating biological samples |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203878152U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105396687A (en) * | 2015-12-04 | 2016-03-16 | 苏州英芮诚生化科技有限公司 | Multi-channel magnetic-material quick transfer magnetic device |
| CN110241009A (en) * | 2019-07-17 | 2019-09-17 | 上海鹍远医疗器械有限公司 | A kind of magnetic force frame module |
-
2014
- 2014-04-01 CN CN201420160909.9U patent/CN203878152U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105396687A (en) * | 2015-12-04 | 2016-03-16 | 苏州英芮诚生化科技有限公司 | Multi-channel magnetic-material quick transfer magnetic device |
| CN110241009A (en) * | 2019-07-17 | 2019-09-17 | 上海鹍远医疗器械有限公司 | A kind of magnetic force frame module |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203030295U (en) | Test tube for mixing and extracting liquid | |
| JP2016502862A5 (en) | ||
| CN202237381U (en) | Novel, simple and easy solid phase extract device | |
| CN203745435U (en) | Special sleeve centrifugal QuEChERS purifier | |
| CN210448175U (en) | Push rod type filtering purification column | |
| CN203878152U (en) | Magnetic frame for separating biological samples | |
| CN203878151U (en) | Sample extractor | |
| CN103551131A (en) | Preparation method of polypropylene film micro solid-phase extraction packet based on MIL-101 adsorbent | |
| CN102507297A (en) | Microwave-assisted Soxhlet and solid-phase extraction coupling device, microwave-assisted Soxhlet and solid-phase extraction coupling method and application of microwave-assisted Soxhlet and solid-phase extraction coupling | |
| CN203878136U (en) | Magnetic system for separating biological samples | |
| CN103175727A (en) | Sample preparation instrument | |
| CN205253190U (en) | Rotation type clinical laboratory uses test -tube rack | |
| CN203999628U (en) | Sample splitter | |
| CN103994914A (en) | Novel method for extracting trace heavy metal ion in environmental water sample | |
| CN201020402Y (en) | Detachable utility type magnetic separating rack | |
| CN108004233A (en) | A kind of negative pressure device available for automatic instrument for extracting nucleic acid | |
| CN205603599U (en) | Magnetic shelf with elastic bulge | |
| CN203355863U (en) | Magnetic separation device for separating biological material | |
| CN207516132U (en) | A kind of multi-functional Tip SPE of high throughput ultramicron | |
| CN206279200U (en) | A kind of townhouse pipe and matching used waste collecting device | |
| CN217324042U (en) | Nucleic acid extraction kit capable of efficiently splitting virus | |
| CN204325348U (en) | A kind of instrument for extracting nucleic acid nucleic acid-extracting apparatus | |
| CN202141701U (en) | Small column for solid phase extraction processing | |
| CN205074084U (en) | Modular small -size desktop formula magnetic separation device of high flux | |
| CN103901143A (en) | Pretreatment method for analyzing tetrabromobisphenol A in small amount of biologic serum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: Hangzhou City, Zhejiang province 311228 Jiangdong Industrial Zone Linjiang high tech Zone weft five road No. 3688 building four layer 2 Branch Park Patentee after: HANGZHOU KBM LIFE SCIENCES CO.,LTD. Address before: 310007, A, East 628, Zhejiang Science Park, 525 Xixi Road, Hangzhou, Zhejiang, Xihu District Patentee before: HANGZHOU KBM LIFE SCIENCES CO.,LTD. |
|
| CP03 | Change of name, title or address | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141015 |
|
| CX01 | Expiry of patent term |