CN205049516U - Adopt unicellular gel electrophoresis to detect kit of DNA chain interruption - Google Patents
Adopt unicellular gel electrophoresis to detect kit of DNA chain interruption Download PDFInfo
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- CN205049516U CN205049516U CN201520741991.9U CN201520741991U CN205049516U CN 205049516 U CN205049516 U CN 205049516U CN 201520741991 U CN201520741991 U CN 201520741991U CN 205049516 U CN205049516 U CN 205049516U
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Abstract
The utility model discloses an adopt unicellular gel electrophoresis to detect kit of DNA chain interruption, including reagent box body, electrophoresis assay gel reagent bottle, lysate reagent bottle, three hole analysis slide glasss have been placed on the internal portion upper strata of kit, the test solution straw has been placed to three hole analysis slide glass both sides, three hole analysis slide glasss below is provided with box body middle level division board, box body middle level division board below is provided with the reagent bottle fixed plate, inside fixed having placed in proper order of reagent bottle fixed plate the lysate reagent bottle, electrophoresis analysis gel reagent bottle, matrix liquid reagent bottle, fluorescent dyes reagent bottle, clearance liquid reagent bottle. Beneficial effect lies in: providing unicellular gel electrophoresis in the kit and having detected required necessary reagent, convenient to use includes a plurality of slide glasss simultaneously, can carry out a plurality of assay experiment simultaneously, improves work efficiency.
Description
Technical field
The utility model belongs to Biological Detection kit field, is specifically related to a kind of kit adopting single cell gel electrophoresis to detect the fracture of DNA chain.
Background technology
Single cell gel electrophoresis detects to analyze and is called Comet Assay again, it is a kind of simple and effective method detecting DNA damage in cell, can be successfully used to detect the fracture of unicellular DNA chain, fast, sensitive, easy, be widely used in DNA shear property model, DNA radiation insult, the detection that DNA is crosslinked, in the work such as the Genotoxic Assessment of medicine and Apoptosis qualification, the process need plurality of reagents of carrying out single cell gel electrophoresis detection coordinates, reagent according to dispersion carries out experiment needs at substantial energy to seek various reagent, testing process is cumbersome, time-consuming effort, based on above reason, need a kind of kit adopting single cell gel electrophoresis to detect the fracture of DNA chain, containing the reagent that is necessary, easy to use.
Utility model content
The purpose of this utility model is just to provide a kind of kit adopting single cell gel electrophoresis to detect the fracture of DNA chain to solve the problem.
The utility model is achieved through the following technical solutions above-mentioned purpose:
A kind of kit adopting single cell gel electrophoresis to detect the fracture of DNA chain, comprise kit box, electrophoresis detection analyzes gel reagents bottle, lysate reagent bottle, the inner upper strata of described kit box is placed with three holes and analyzes microslide, described three holes are analyzed microslide both sides and are placed with test solution suction pipe, described three holes are analyzed below microslide and are provided with box body middle level demarcation strip, reagent bottle fixed head is provided with below the demarcation strip of described box body middle level, the inner fixed placement of described reagent bottle fixed head has described lysate reagent bottle, described lysate reagent bottle side fixed placement has described electrophoresis detection to analyze gel reagents bottle, described electrophoresis detection analyzes gel reagents bottle side fixed placement matrix liquid reagent bottle, described matrix liquid reagent bottle side fixed placement has fluorescent dye reagent bottle, described fluorescent dye reagent bottle side fixed placement has cleaning liquid reagent bottle.
In said structure, described kit box holds the various reagent and instrument of testing, described three holes are analyzed microslide and are placed test samples, in the bottle of described lysate reagent bottle, described electrophoresis detection analysis gel reagents bottle, described matrix liquid reagent bottle, described fluorescent dye reagent bottle and described cleaning liquid reagent bottle, detection reagent is housed respectively, join in sample according to different order, different amounts, carry out detected through gel electrophoresis.
As preferred version of the present utility model, be placed with described lysate reagent bottle in the fixed orifice on described reagent bottle fixed head successively, described electrophoresis detection analyzes gel reagents bottle.
As preferred version of the present utility model, described matrix liquid reagent bottle, described fluorescent dye reagent bottle and described cleaning liquid reagent bottle are placed in the fixed orifice on described reagent bottle fixed head successively.
The beneficial effects of the utility model are: provide the reagent that is necessary needed for single cell gel electrophoresis detection in kit, easy to use, include multiple microslide simultaneously, can carry out multiple analysis design mothod simultaneously, increase work efficiency.
Accompanying drawing explanation
Fig. 1 is a kind of front view adopting single cell gel electrophoresis to detect the kit of DNA chain fracture described in the utility model;
Fig. 2 is a kind of vertical view adopting single cell gel electrophoresis to detect the kit of DNA chain fracture described in the utility model.
1, kit box; 2, box body middle level demarcation strip; 3, reagent bottle fixed head; 4, liquid reagent bottle is cleared up; 5, fluorescent dye reagent bottle; 6, matrix liquid reagent bottle; 7, electrophoresis detection analyzes gel reagents bottle; 8, lysate reagent bottle; 9, microslide is analyzed in three holes; 10, test solution suction pipe;
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further detail:
As Figure 1-Figure 2, a kind of kit adopting single cell gel electrophoresis to detect the fracture of DNA chain, comprise kit box 1, electrophoresis detection analyzes gel reagents bottle 7, lysate reagent bottle 8, the inner upper strata of kit box 1 is placed with three holes and analyzes microslide 9, kit box 1 is in order to hold the various reagent and instrument of testing, microslide 9 is analyzed in order to place sample to be detected in three holes, other reagent can be added to be above convenient to carry out testing and observing simultaneously, three holes are analyzed microslide 9 both sides and are placed with test solution suction pipe 10, test solution in order to extract reagent bottle drips and is put on microslide, three holes are analyzed below microslide 9 and are provided with box body middle level demarcation strip 2, in order to box body to be divided into the different article of upper and lower two-layer placement, reagent bottle fixed head 3 is provided with below box body middle level demarcation strip 2, the inner fixed placement of reagent bottle fixed head 3 has lysate reagent bottle 8, unicellular in order to cracking, lysate reagent bottle 8 side fixed placement has electrophoresis detection to analyze gel reagents bottle 7, in order to carry out colloid condense by containing the unicellular of DNA, be convenient to carry out cracking and subsequent analysis, electrophoresis detection analyzes gel reagents bottle 7 side fixed placement matrix liquid reagent bottle 6, in order to deposit the unicellular of extraction, ensure that it is active, matrix liquid reagent bottle 6 side fixed placement has fluorescent dye reagent bottle 5, observation analysis is convenient in order to carry out DNA dyeing, fluorescent dye reagent bottle 5 side fixed placement has cleaning liquid reagent bottle 4, slide is cleared up after detecting, different detection reagent is housed in these reagent bottles respectively, according to different order in testing process, different amounts joins in sample, cooperatively interact and carry out single cell gel electrophoresis detection.
In said structure, kit box 1 holds the various reagent and instrument of testing, three holes are analyzed microslide 9 and are placed test samples, lysate reagent bottle 8, electrophoresis detection are analyzed gel reagents bottle 7, matrix liquid reagent bottle 6, fluorescent dye reagent bottle 5 and are cleared up in the bottle of liquid reagent bottle 4 and detection reagent is housed respectively, join in sample according to different order, different amounts, carry out detected through gel electrophoresis.
As preferred version of the present utility model, in the fixed orifice on reagent bottle fixed head 3, be placed with lysate reagent bottle 8, electrophoresis detection analysis gel reagents bottle 7, matrix liquid reagent bottle 6, fluorescent dye reagent bottle 5 and cleaning liquid reagent bottle 4 successively.
More than show and describe ultimate principle of the present utility model, principal character and advantage.The technician of the industry should understand; the utility model is not restricted to the described embodiments; what describe in above-described embodiment and instructions just illustrates principle of the present utility model; under the prerequisite not departing from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.The claimed scope of the utility model is defined by appending claims and effect thing thereof.
Claims (3)
1. the kit adopting single cell gel electrophoresis to detect the fracture of DNA chain, it is characterized in that: comprise kit box, electrophoresis detection analyzes gel reagents bottle, lysate reagent bottle, the inner upper strata of described kit box is placed with three holes and analyzes microslide, described three holes are analyzed microslide both sides and are placed with test solution suction pipe, described three holes are analyzed below microslide and are provided with box body middle level demarcation strip, reagent bottle fixed head is provided with below the demarcation strip of described box body middle level, the inner fixed placement of described reagent bottle fixed head has described lysate reagent bottle, described lysate reagent bottle side fixed placement has described electrophoresis detection to analyze gel reagents bottle, described electrophoresis detection analyzes gel reagents bottle side fixed placement matrix liquid reagent bottle, described matrix liquid reagent bottle side fixed placement has fluorescent dye reagent bottle, described fluorescent dye reagent bottle side fixed placement has cleaning liquid reagent bottle.
2. a kind of kit adopting single cell gel electrophoresis to detect the fracture of DNA chain according to claim 1, is characterized in that: be placed with described lysate reagent bottle in the fixed orifice on described reagent bottle fixed head successively, described electrophoresis detection analyzes gel reagents bottle.
3. a kind of kit adopting single cell gel electrophoresis to detect the fracture of DNA chain according to claim 1, is characterized in that: described matrix liquid reagent bottle, described fluorescent dye reagent bottle and described cleaning liquid reagent bottle are placed in the fixed orifice on described reagent bottle fixed head successively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520741991.9U CN205049516U (en) | 2015-09-23 | 2015-09-23 | Adopt unicellular gel electrophoresis to detect kit of DNA chain interruption |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520741991.9U CN205049516U (en) | 2015-09-23 | 2015-09-23 | Adopt unicellular gel electrophoresis to detect kit of DNA chain interruption |
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| CN205049516U true CN205049516U (en) | 2016-02-24 |
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| CN201520741991.9U Active CN205049516U (en) | 2015-09-23 | 2015-09-23 | Adopt unicellular gel electrophoresis to detect kit of DNA chain interruption |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017214822A1 (en) * | 2016-06-13 | 2017-12-21 | 石庆学 | Sample adding plate for single cell gel electrophoresis |
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2015
- 2015-09-23 CN CN201520741991.9U patent/CN205049516U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017214822A1 (en) * | 2016-06-13 | 2017-12-21 | 石庆学 | Sample adding plate for single cell gel electrophoresis |
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