CN201955342U

CN201955342U - Fluorescence detection kit for asialoglycoprotein receptor (ASGPR)

Info

Publication number: CN201955342U
Application number: CN2011200122778U
Authority: CN
Inventors: 付金瑞; 王高升
Original assignee: Tianjin Baodi Hospital
Current assignee: Tianjin Baodi Hospital
Priority date: 2011-01-14
Filing date: 2011-01-14
Publication date: 2011-08-31
Anticipated expiration: 2021-01-14

Abstract

The utility model relates to a fluorescence detection kit for an asialoglycoprotein receptor (ASGPR). A cover is connected above a kit body, a cover glass box and a glass slide box are fixed at the left end in the kit body side by side, two rows of reagent bottles are arranged at other parts in the kit body, the reagent bottles are respectively provided with bottle caps, a sponge is arranged at the bottom of the kit body, and the sponge is provided with holes with same diameter as the longitudinal cross section of the cover glass box and the glass slide box and the reagent bottles for locating. Four square grooves are made on a glass slide, the size of the square groove is 3*3*1mm, and the trace purified ASGPR is fixed on the bottom surface of the groove. The fluorescence detection kit has the advantages that all the reagents (including critical positive quality-control serum), the glass slide and the cover glass used in detection are gathered together, the operation is simple, the cost is low, the result is accurate, and the detection time is about 1.5 hours.

Description

Be used for the anti-asialoglycoprotein receptor fluorescence detection reagent kit of human serum

Technical field

The utility model relates to a kind of kit, particularly relates to a kind of anti-asialoglycoprotein receptor fluorescence detection reagent kit of human serum that is used for.

Background technology

(Asialoglycoprotein Receptor is hepatocellular a kind of important and endocytosis acceptor efficiently ASGPR) to the anti-asialoglycoprotein receptor of human serum, mainly is distributed in around the lobuli hepatis portal vein on the hepatocellular hole facial mask.Anti-ASGPR antibody is the specific autoantibody of oneself immunity hepatitis (AIH) diagnosis, as the index of judging disease activity degree, treatment monitoring and judging prognosis, is called as " barometer " of AIH disease activity; The ASGPR antibody detection method is mainly radioimmunology (RIA) and enzyme immunoassay (EIA) at present, technical requirement and detection cost are higher, be difficult to popularize, especially when detecting, employed article all disperse to place, and not only take by mistake easily in the work, also waste time, influence work efficiency, increased labour intensity.

Summary of the invention

The utility model provides a kind of being easy to apply for solving the technical matters that exists in the known technology, and testing result is accurate and with low cost is used for the anti-asialoglycoprotein receptor fluorescence detection reagent kit of human serum.

The technical scheme that the utility model is taked for the technical matters that exists in the solution known technology is, it comprises box body and lid, it is characterized in that: the box body top is connected with lid, left end in the box body is fixed cap slide box and Glass carrier box side by side, two row's reagent bottles are laid at other position in the box body, and reagent bottle all has bottle cap, is placed with sponge in the bottom of box body, have on the sponge and cover glass box, the Glass carrier box longitudinal cross-section hole identical, in order to the location with the reagent bottle diameter.Be manufactured with four square grooves on the microslide, the size of square groove is 3 * 3 * 1mm, and the lower surface of groove is fixed with micro-purifying asialoglycoprotein receptor.

The utility model can also be by the following technical solutions:

Described groove is square or circular, and its square groove is of a size of 3 * 3 * 1mm, and its circular groove is of a size of ￠ 3 * 1mm;

Described box body, lid adopt plasticizing waterproof paper material, and the material of cover glass box and Glass carrier box is a rigid plastic;

Advantage and the good effect that the utlity model has are: reagent (comprising critical positive quality control serum), microslide and the cover glass etc. that use when all detect all concentrate in together, and are simple to operate, with low cost, the result is accurate, be easy to promote, detection time is shorter, is generally 1.5 hours.

Description of drawings

Fig. 1 is the utility model one-piece construction synoptic diagram;

Fig. 2 is the slide configurations synoptic diagram;

Fig. 3 is the sponge structure synoptic diagram.

Among Fig. 1-Fig. 3: 1. box body, 2. lid, 3. cover glass box, 4. Glass carrier box,

5 reagent bottles, 6. bottle cap, 7. sponge 8. microslides, 9. groove.

Embodiment

For further understanding summary of the invention of the present utility model, characteristics and effect, exemplify following examples now, and conjunction with figs. is described in detail as follows, see also Fig. 1-Fig. 3.

As Fig. 1-shown in Figure 3: box body 1 top is connected with lid 2, left end in the box body 1 is fixed cap slide box 3 and Glass carrier box 4 side by side, two row's reagent bottles 5 are laid at other position in the box body 1, reagent bottle 5 all has bottle cap 6, be placed with sponge 7 in the bottom of box body 1, have on the sponge 7 with cover glass box 3 and Glass carrier box 4 longitudinal cross-sections and with the identical hole of reagent bottle 5 diameters; Be manufactured with four circular grooves 9 that are of a size of the square groove 9 of 3 * 3 * 1mm or are of a size of ￠ 3 * 1mm on the described microslide 8, the lower surface of groove 9 is fixed with micro-purifying asialoglycoprotein receptor; Described box body 1, lid 2 adopt plasticizing waterproof paper material, and the material of cover glass box 3 and Glass carrier box 4 is a rigid plastic; During use, the each detection needs to do the parallel control detection with the critical positive quality control serum that kit provides; Get respectively in right amount through the tested serum of 10 times of dilutions of dilution that kit provides and critical positive quality control serum (directly with) and join in the different grooves 9, temperature drips reagent A respectively after bathing flushing, temperature drips reagent B respectively after bathing flushing, temperature adds mountant after bathing flushing, can observe under fluorescent microscope with the cover plate mounting and determine testing result.

Its advantage is: reagent (comprising critical positive quality control serum), microslide and the cover glass etc. that use when all detect all concentrate in together, simple to operate, with low cost, overcome in tester's subjective factor and the testing process multiple influence factor to the influence of testing result, the result is accurate, and detection time, weak point was generally 1.5 hours.

Above-described embodiment is described preferred implementation of the present utility model; be not that scope of the present utility model is limited; under the prerequisite that does not break away from the utility model design spirit; various distortion and improvement that those of ordinary skills make the technical solution of the utility model all should fall in the definite protection domain of the utility model claims.

Claims

1. anti-asialoglycoprotein receptor fluorescence detection reagent kit of human serum, it comprises box body and lid, it is characterized in that: box body (1) top is connected with lid (2), left end in the box body (1) is fixed cap slide box (3) and Glass carrier box (4) side by side, two row's reagent bottles (5) are laid at other position in the box body (1), reagent bottle (5) all has bottle cap (6), be placed with sponge (7) in the bottom of box body (1), have on the sponge (7) with cover glass box (3) and Glass carrier box (4) longitudinal cross-section and with the identical hole of reagent bottle (5) diameter, be manufactured with four grooves (9) on the described microslide (8).

2. the anti-asialoglycoprotein receptor fluorescence detection reagent kit of human serum according to claim 1, it is characterized in that: described groove (9) is square or circular, its square groove is of a size of 3 * 3 * 1mm, and its circular groove is of a size of 3 * 1mm.

3. the anti-asialoglycoprotein receptor fluorescence detection reagent kit of human serum according to claim 1, it is characterized in that: described box body (1), lid (2) adopt plasticizing waterproof paper material, and the material of cover glass box (3) and Glass carrier box (4) is a rigid plastic.