CN203705389U - Visualized microimaging nanochannel detection pool - Google Patents
Visualized microimaging nanochannel detection pool Download PDFInfo
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- CN203705389U CN203705389U CN201320622708.1U CN201320622708U CN203705389U CN 203705389 U CN203705389 U CN 203705389U CN 201320622708 U CN201320622708 U CN 201320622708U CN 203705389 U CN203705389 U CN 203705389U
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- liquid passage
- flow liquid
- support plate
- chip support
- nanochannel
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- 239000002090 nanochannel Substances 0.000 title claims abstract description 42
- 238000001514 detection method Methods 0.000 title claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 80
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 28
- 230000000007 visual effect Effects 0.000 claims description 18
- 238000003384 imaging method Methods 0.000 claims description 17
- 238000010276 construction Methods 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 3
- 239000010453 quartz Substances 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 230000008569 process Effects 0.000 abstract description 4
- 239000000758 substrate Substances 0.000 abstract 3
- 241000276425 Xiphophorus maculatus Species 0.000 abstract 2
- 230000008859 change Effects 0.000 description 4
- 238000000835 electrochemical detection Methods 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 239000011148 porous material Substances 0.000 description 4
- 230000009977 dual effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- UELITFHSCLAHKR-UHFFFAOYSA-N acibenzolar-S-methyl Chemical compound CSC(=O)C1=CC=CC2=C1SN=N2 UELITFHSCLAHKR-UHFFFAOYSA-N 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005295 random walk Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000004557 single molecule detection Methods 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a visualized microimaging nanochannel detection pool which comprises a main board, upper and lower chip loading plates and a substrate, wherein a main channel opening and an assistant channel opening are formed in the rectangular main board with a slot structure on the reverse face, and an upper main liquid flowing channel and an upper assistant liquid flowing channel are arranged on the upper chip loading plate in a rectangular platy structure; a lower main liquid flowing channel and a lower assistant liquid flowing channel are arranged on the lower chip loading plate in a rectangular platy structure; a main communicating pool and an assistant communicating pool connected through a communicating groove are arranged on the substrate with a rectangular groove in the front face, and the upper and lower chip loading plates can be buckled to form an integral structure by the main board and the substrate through the groove structure; the main channel opening is communicated with the upper and lower main liquid flowing channels and the main communicating pool up and down; the assistant channel opening is communicated with the upper and lower assistant liquid flowing channels and the assistant communicating pool up and down. The nanochannel detection pool provided by the utility model is easy to process and produce on a large scale and suitable for different detection objects and detection conditions in the field of electroanalysis detection of the nanochannel.
Description
Technical field
The utility model relates to nanochannel electroanalysis detection technique field, specifically, relates to and makes a kind of visual micro-imaging nanochannel detection cell that can be used for microscopic nanochannel real-time status.
Background technology
Detect analysis technical field at nanochannel electroanalysis, nanochannel electrochemical measuring technique (Nanopore Technique) is to utilize the technology that detects faint ion current feature electric signal that individual molecule produces during through the passage of nano-scale under electric field driven and study biomolecule individual behavior.The electricity that has changed hole due to the physics occupy-place effect of single testing molecule in nano-pore is led, thereby causes that the gas current of the nano-pore of flowing through changes, and forms blocking-up current signal.And each blocking-up current signal strength of current, Xining and signal shape the individual behavior information in nano-pore is directly related with described individual molecule.By the statistical treatment to a large amount of single blocking-up current signals, analyze variation spoke value, duration and the frequency of single blocking-up electric current event, can realize the deciphering to single large molecule individual behavior information.By the hypersensitive record to weak current signal, can directly obtain the change information of individual molecule, on single molecules level, obtain in real time, with sensitivity the information such as biomolecule conformation change dynamics, intermolecular weak interaction.Nanometer pore single-molecule electrochemical measuring technique, without probe mark, directly just can be monitored the interaction between single bion behavior and biomolecule by monitoring ion blocking-up electric current.
At present, nanochannel molecule detection only can record skin peace level blocking-up current signal in the time adopting commercial nanochannel instrument, and the observation of the detailed information that nanochannel is around occurred is very limited.In addition, the Comparison of experiment results complexity that the chance phenomenon of biomolecule (Random Walk) often makes this technology obtain, analytic process is relatively loaded down with trivial details.Therefore, need in the process of Single Molecule Detection, provide more analysis data and signal, especially visual image information.Key problem is wherein exactly: need design and prepare a kind ofly can coordinate microscope uses, the device that can carry out to nanochannel analyzing and testing pond visual inspection, to realize when nanochannel is carried out to Electrochemical Detection record molecule by the image of nanochannel process.
Utility model content
The purpose of this utility model is to solve above-described problem, a kind of visual micro-imaging nanochannel detection cell that is positioned over microscope stage and can be used for optical observation nanochannel real-time status is provided, described nanochannel detection cell can be applicable to the detection of conformation change and the weak interaction of biomolecule, individual molecule is carried out when by nanochannel the dual signal collection of electric current and image in nanochannel Electrochemical Detection.
For achieving the above object, the utility model has been taked following technical scheme.
A kind of visual micro-imaging nanochannel detection cell, contain mainboard, upper chip support plate, lower chip support plate and pedestal, it is characterized in that, have rectangle flute profile structure at the reverse side of described mainboard, in described bathtub construction, be provided with as the main channel opening of camera lens opening with for the subaisle opening of electrodes, be provided with connection at the two ends of described mainboard protruding, on described connection is protruding, be provided with the hole of tightening up a screw;
Described upper chip support plate is the tabular structure of rectangle that is less than described bathtub construction internal diameter, on the chip support plate position same with described main channel opening and subaisle opening, be provided with on main flow liquid passage on described and secondary flow liquid passage on, a circular groove for having at the reverse side of upper chip support plate on described main flow liquid passage, the structure that has a through hole in the middle of described circular groove is a through hole on upper chip support plate on described secondary flow liquid passage;
Described lower chip support plate is the rectangle tabular structure identical with described upper chip support plate shape, described lower chip support plate with on described main flow liquid passage and on secondary flow liquid passage, on same position, be provided with under main flow liquid passage and secondary flow liquid passage under, a circular groove for having in the front of lower chip support plate under described main flow liquid passage, the structure that has a through hole in the middle of described circular groove is identical with the diameter on described main flow liquid passage under described main flow liquid passage; Under described secondary flow liquid passage, be a through hole on lower chip support plate, identical with the diameter on described secondary flow liquid passage under described secondary flow liquid passage;
Described pedestal is the structural member that has rectangular recess in the centre in its front, described groove is identical with the rectangle flute profile structure of described mainboard reverse side, in described groove, be provided with the main connection pond identical with subaisle aperture position with described main channel opening and the secondary pond that is communicated with, described main connection pond is connected with the secondary pond that is communicated with by connectivity slot, is provided with lower screw hole at the two ends of described pedestal;
Described mainboard can be buckled into an one-piece construction by its rectangle flute profile structure and described pedestal by described upper chip support plate and described lower chip support plate by its rectangular recess, couple together with described lower screw hole by the described hole of tightening up a screw with screw: described main channel opening with on described main flow liquid passage, under main flow liquid passage, the main pond that is communicated with is communicated with up and down, described subaisle opening with on described secondary flow liquid passage, under secondary flow liquid passage, be secondaryly communicated with pond and be communicated with up and down
Further, optionally, described mainboard, upper chip support plate, lower chip support plate and pedestal are quartz or glass structure part.
Further, long 30～120mm, wide 20～100mm of described mainboard, high 4～30mm; Described pedestal long 30～120mm, wide 10～60mm, high 4～30mm.
Further, the diameter of described main channel opening is 8～20 mm, and the diameter of subaisle opening is 3～12mm.
Further, identical with the diameter under main flow liquid passage on described main flow liquid passage, be all 5～15mm.
Further, identical with the diameter under secondary flow liquid passage on described secondary flow liquid passage, be all 4～13mm.
Further, the diameter in described main connection pond is 3～12mm; The described secondary diameter that is communicated with pond is 1～10mm.
Further, described connection flute length 10～25mm, wide 0.5～6mm.
The good effect of a kind of visual micro-imaging nanochannel detection cell of the utility model is:
(1) simple structure, mainboard by containment member and pedestal can seal effectively installs chip support plate and lower chip support plate, form fixing nanochannel, realized integrated by nanochannel detection cell of the present utility model (sensor) and microscopic system by optical path.
(2) can, in nanochannel Electrochemical Detection, when by nanochannel, carry out the dual signal collection of electric current and image to individual molecule by microscope.
(3) be easy to processing, be easy to batch production, can be applicable to different detected objects and testing conditions.
Brief description of the drawings
Fig. 1 is the structural representation of a kind of visual micro-imaging nanochannel detection cell of the utility model.
Label in figure is respectively:
1, mainboard; 2, upper chip support plate;
3, lower chip support plate; 4, pedestal;
5, main channel opening; 6, subaisle opening;
7, screw; 8, the hole of tightening up a screw;
9, on main flow liquid passage; 10, on secondary flow liquid passage;
11, under main flow liquid passage; 12, under secondary flow liquid passage;
13, main connection pond; 14, the secondary pond that is communicated with;
15, connectivity slot; 16, lower screw hole.
Embodiment
Provide the embodiment of a kind of visual micro-imaging nanochannel detection cell of the utility model below in conjunction with accompanying drawing, but it should be pointed out that enforcement of the present utility model is not limited to following embodiment.
Referring to Fig. 1.A kind of visual micro-imaging nanochannel detection cell, contains mainboard 1, upper chip support plate 2, lower chip support plate 3 and pedestal 4, and described mainboard 1, upper chip support plate 2, lower chip support plate 3 and pedestal 4 are quartz or glass structure part.Described mainboard 1 is identical with the physical dimension of described pedestal 4, and (in enforcement, described mainboard 1 can adopt the structural member of long 30～120mm, wide 20～100mm, high 4～30mm scope to be the structural member of long 70mm, wide 30mm, high 5mm; Described pedestal 4 can adopt the structural member of long 30～120mm, wide 10～60mm, high 4～30mm scope).
Set a rectangle flute profile structure at the reverse side of described mainboard 1, a main channel opening 5 as camera lens opening and a subaisle opening 6 for electrodes are set in described bathtub construction, the diameter of described main channel opening 5 is 12 mm, the diameter of described subaisle opening 6 is during 7.42mm(implements, the diameter of described main channel opening 5 can be the scope of 8～20mm, and the diameter of described subaisle opening 6 can be the scope of 3～12mm).A connection is respectively set protruding at the two ends of described mainboard 1, the hole 8 of tightening up a screw is set on described connection is protruding.Described mainboard 1, rectangle flute profile structure, connect the protruding and hole 8 of tightening up a screw and can the method by global formation make.
Described upper chip support plate 2 adopts the tabular structure of rectangle that is less than described bathtub construction internal diameter, that is, described upper chip support plate 2 can embed in the rectangle flute profile structure of described mainboard 1 reverse side.
On chip support plate 2 position same with described main channel opening 5 and subaisle opening 6, arrange on described on main flow liquid passage 9 and secondary flow liquid passage on 10.On described main flow liquid passage, 9 for being arranged on a circular groove of chip support plate 2 reverse side, in the middle of described circular groove, sets a through hole, and on described main flow liquid passage, 9 diameter is 10mm.On described secondary flow liquid passage, 10 is a through hole on upper chip support plate 2, and diameter is 8mm.
Described lower chip support plate 3 is and the tabular structural member of rectangle of described upper chip support plate 2 similar shapes, with on described main flow liquid passage 9 and secondary flow liquid passage on arrange on 10 same positions under main flow liquid passage 11 and secondary flow liquid passage under 12.Under described main flow liquid passage, 11 for being arranged on a circular groove in lower chip support plate 3 fronts, in the middle of described circular groove, set a through hole, under described main flow liquid passage, 11 is identical with 9 diameter on described main flow liquid passage, for during 10mm(implements, on described main flow liquid passage 9 with described main flow liquid passage under 11 diameter can be the scope of 5～15mm).Under described secondary flow liquid passage, 12 is a through hole on lower chip support plate 3, diameter 8mm, identical with 10 diameter on described secondary flow liquid passage (in enforcement, on described secondary flow liquid passage 10 with described secondary flow liquid passage under 12 diameter can be the scope of 4～13mm).
The length of described pedestal 4 is identical with the length (connection that comprises its two ends is protruding) of described mainboard 1.Set a rectangular recess in the centre in pedestal 4 fronts, the structure of described groove is identical with the rectangle flute profile structure of described mainboard 1 reverse side.The main connection pond identical with subaisle opening 6 positions with described main channel opening 5 13 and the secondary pond 14 that is communicated with are set in described groove, and the diameter in described main connection pond 13 is that during 5mm(implements, the diameter in described main connection pond 13 can be the scope of 3～12mm); The described secondary diameter that is communicated with pond 14 is during 3mm(implements, and the described secondary diameter that is communicated with pond 14 can be the scope of 1～10mm).Described main connection pond 13 is connected with the secondary pond 14 that is communicated with by connectivity slot 15, and during the long 12.97mm of described connectivity slot 15, wide 0.5mm(implement, the length of described connectivity slot 15 can be the scope of 10～25mm, the wide 0.5～6mm of being).Lower screw hole 16 is set at the two ends of described pedestal 4.Described pedestal 4, rectangular recess and lower screw hole 16 can be made by the method for global formation.
When use, described mainboard 1 can be buckled into an one-piece construction by its rectangle flute profile structure and described pedestal 4 by described upper chip support plate 2 and described lower chip support plate 3 by its rectangular recess, couple together with described lower screw hole 16 by the described hole 8 of tightening up a screw with screw 7: described main channel opening 5 with on described main flow liquid passage 9, under main flow liquid passage 11, the main pond 13 that is communicated with is communicated with up and down, described subaisle opening 6 with on described secondary flow liquid passage 10, under secondary flow liquid passage 12, be secondaryly communicated with pond 14 and be communicated with up and down.Microscope camera lens can go deep into observing nanochannel thin slice in main channel opening 5, can electrodes in subaisle opening 6.Solution in detection cell via on main flow liquid passage 9, under main flow liquid passage 11, main connection pond 13, connectivity slot 15 with is secondaryly communicated with under pond 14, secondary flow liquid passage 12,10 connections on secondary flow liquid passage.
Visual micro-imaging nanochannel detection cell of the present utility model can be used for the detection of conformation change and the weak interaction of biomolecule; For individual molecule being carried out when by nanochannel the dual signal collection of electric current and image in nanochannel Electrochemical Detection.
The foregoing is only preferred implementation of the present utility model; should be understood that; for those skilled in the art; do not departing under the prerequisite of the utility model structure; can also make some improvement and modification, these improvement and modification also should be considered as the scope of the utility model protection.
Claims (8)
1. a visual micro-imaging nanochannel detection cell, contain mainboard (1), upper chip support plate (2), lower chip support plate (3) and pedestal (4), it is characterized in that, have rectangle flute profile structure at the reverse side of described mainboard (1), in described bathtub construction, be provided with as the main channel opening (5) of camera lens opening with for the subaisle opening (6) of electrodes, be provided with connection at the two ends of described mainboard (1) protruding, on described connection is protruding, be provided with the hole of tightening up a screw (8);
Described upper chip support plate (2) is for being less than the tabular structure of rectangle of described bathtub construction internal diameter, on chip support plate (2) position same with described main channel opening (5) and subaisle opening (6), be provided with on described on main flow liquid passage on (9) and secondary flow liquid passage (10), (9) circular groove for having at the reverse side of upper chip support plate (2) on described main flow liquid passage, the structure that has a through hole in the middle of described circular groove, on described secondary flow liquid passage, (10) are a through hole on upper chip support plate (2);
Described lower chip support plate (3) is the rectangle tabular structure identical with described upper chip support plate (2) shape, on described lower chip support plate (3) position same with (10) on (9) on described main flow liquid passage and secondary flow liquid passage, be provided with under main flow liquid passage under (11) and secondary flow liquid passage (12), (11) circular groove for having in the front of lower chip support plate (3) under described main flow liquid passage, in the middle of described circular groove, have the structure of a through hole, under described main flow liquid passage, (11) are identical with the diameter of (9) on described main flow liquid passage, under described secondary flow liquid passage, (12) are a through hole on lower chip support plate (3), and under described secondary flow liquid passage, (12) are identical with the diameter of (10) on described secondary flow liquid passage,
Described pedestal (4) is for having the structural member of rectangular recess in the centre in its front, described groove is identical with the rectangle flute profile structure of described mainboard (1) reverse side, in described groove, be provided with the main connection pond (13) identical with subaisle opening (6) position with described main channel opening (5) and the secondary pond (14) that is communicated with, described main connection pond (13) is connected with the secondary pond (14) that is communicated with by connectivity slot (15), is provided with lower screw hole (16) at the two ends of described pedestal (4);
Described mainboard (1) can be buckled into an one-piece construction by its rectangle flute profile structure and described pedestal (4) by described upper chip support plate (2) and described lower chip support plate (3) by its rectangular recess, couple together by the described hole of tightening up a screw (8) and described lower screw hole (16) with screw (7): on described main channel opening (5) and described main flow liquid passage (9), under main flow liquid passage (11), main connection pond (13) is communicated with up and down, on described subaisle opening (6) and described secondary flow liquid passage (10), under secondary flow liquid passage (12), the secondary pond (14) that is communicated with is communicated with up and down.
2. the visual micro-imaging nanochannel of one according to claim 1 detection cell, is characterized in that, described mainboard (1), upper chip support plate (2), lower chip support plate (3) and pedestal (4) are quartz or glass structure part.
3. the visual micro-imaging nanochannel of one according to claim 1 and 2 detection cell, is characterized in that, described mainboard (1) long 30～120mm, wide 20～100mm, high 4～30mm; Described pedestal (4) long 30～120mm, wide 10～60mm, high 4～30mm.
4. the visual micro-imaging nanochannel of one according to claim 1 and 2 detection cell, is characterized in that, the diameter of described main channel opening (5) is 8～20 mm, and the diameter of described subaisle opening (6) is 3～12mm.
5. the visual micro-imaging nanochannel of one according to claim 1 and 2 detection cell, is characterized in that, on described main flow liquid passage, (9) are identical with the diameter of (11) under described main flow liquid passage, are all 5～15mm.
6. the visual micro-imaging nanochannel of one according to claim 1 and 2 detection cell, is characterized in that, on described secondary flow liquid passage, (10) are identical with the diameter of (12) under described secondary flow liquid passage, are all 4～13mm.
7. the visual micro-imaging nanochannel of one according to claim 1 and 2 detection cell, is characterized in that, the diameter in described main connection pond (13) is 3～12mm; The described secondary diameter that is communicated with pond (14) is 1～10mm.
8. the visual micro-imaging nanochannel of one according to claim 1 and 2 detection cell, is characterized in that, the long 10～25mm of described connectivity slot (15), wide 0.5～6mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320622708.1U CN203705389U (en) | 2013-10-10 | 2013-10-10 | Visualized microimaging nanochannel detection pool |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320622708.1U CN203705389U (en) | 2013-10-10 | 2013-10-10 | Visualized microimaging nanochannel detection pool |
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| Publication Number | Publication Date |
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| CN203705389U true CN203705389U (en) | 2014-07-09 |
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| CN201320622708.1U Expired - Fee Related CN203705389U (en) | 2013-10-10 | 2013-10-10 | Visualized microimaging nanochannel detection pool |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107436317A (en) * | 2017-07-21 | 2017-12-05 | 河南金泰生物技术股份有限公司 | A kind of method using nanochannel technology for detection cancer |
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2013
- 2013-10-10 CN CN201320622708.1U patent/CN203705389U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107436317A (en) * | 2017-07-21 | 2017-12-05 | 河南金泰生物技术股份有限公司 | A kind of method using nanochannel technology for detection cancer |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140709 |
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| CF01 | Termination of patent right due to non-payment of annual fee |