CN208266182U - Full-automatic in situ hybridization device - Google Patents
Full-automatic in situ hybridization device Download PDFInfo
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- CN208266182U CN208266182U CN201820343172.2U CN201820343172U CN208266182U CN 208266182 U CN208266182 U CN 208266182U CN 201820343172 U CN201820343172 U CN 201820343172U CN 208266182 U CN208266182 U CN 208266182U
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- situ hybridization
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Abstract
The utility model discloses a kind of full-automatic in situ hybridization devices, comprising: sample reactive tank, for placing sample；Reactive tank shaking table portion is located at below sample reactive tank, for swinging sample reactive tank；Selective feed liquor portion and selective drain portion, connect with sample reactive tank, reagent are added to samples selection of the selective feed liquor portion into sample reactive tank, the reagent after reaction is discharged from selective drain portion；Temperature adjustment portion carries out heating, cooling to sample reactive tank or keeps constant temperature；Control unit, the amplitude of fluctuation and time, reagent for controlling sample reactive tank are added and the reaction time of the type of discharge and time, the time of the progress heating, cooling of sample reactive tank or holding constant temperature and temperature and sample, to be automatically performed Sample pretreatment, denaturing samples hybridization and sample final wash.The utility model is the efficient experimental provision of electromechanical integration, it can realize all kinds of hybridization in situ experiment whole process automations, standardization, high efficiency.
Description
Technical field
The utility model relates to field of biotechnology, and in particular, to a kind of full-automatic in situ hybridization device.
Background technique
With the continuous development of Protocols in Molecular Biology, in situ hybridization has obtained day in terms of molecular pathology and scientific research
The extensive promotion and application of benefit.Characteristic of the in situ hybridization based on DNA double chain complementation, by fluorescein-labeled DNA fragmentation (probe)
Specific hybrid then passes through in fluorescence microscope and observes under suitable optical filter on target nucleic acid sequence (chromosome or gene)
The dazzling fluorescence signal being integrated in nucleus on target detects a certain particular target nucleic acid sequence variation with this, therefore, it is determined that
A kind of technology of its structure and numerical abnormality.The basic principle is that two nucleotide single-chain segments under appropriate conditions can mistake
The characteristics of Hydrogenbond, formation DNA-DNA, DNA-RNA or RNA-RNA double bond molecule, (there is radioactivity same using with markd
Position element, such as 3H, 35S, 32P, fluorescein biotin, digoxin non-radioactive substance) DNA or RNA segment as nucleic acid probe,
Hybridized with histotomy or intracellular determined nucleic acid (RNA or DNA) segment, then the methods of autoradiograph can be used to give
It has been shown that, presence and positioning in light microscopic or electric microscopic observation purpose mRNA or DNA；With hybridization in situ technique, can study in situ
Cell synthesizes the gene expression of certain polypeptide or protein.The method has very high sensibility and specificity, can further from point
Sub- level inquires into the functional expression and its adjustment mechanism of cell.Have become current cell biology, molecular biology research
Important means.
Current in situ hybridization device is mainly operated using artificial long period of experiments, in conjunction with common only heating function
In situ hybridization instrument completes hybridization in situ experiment.Time is long, the degree of automation is low, manual intervention is excessive, cannot achieve standardized stream
Journey and Quality Control.
Utility model content
In view of problem above, the purpose of the utility model is to provide a kind of full-automatic in situ hybridization devices, it can be achieved that all kinds of
The automation of hybridization in situ experiment whole process, standardization, high efficiency.
The utility model discloses a kind of in situ hybridization devices, including sample reactive tank, for placing sample；Reactive tank shakes
Bed portions are located at below the sample reactive tank, for swinging the sample reactive tank；Selective feed liquor portion and selective drain
Portion, the selectivity feed liquor portion and the selective drain portion are connect with the sample reactive tank, the selective feed liquor portion to
Reagent is added to samples selection in the sample reactive tank, the reagent after reaction is discharged from the selective drain portion；It adjusts
Warm portion carries out heating, cooling to the sample reactive tank or keeps constant temperature；And control unit, with the sample reactive tank, described
Reactive tank shaking table portion, the selective feed liquor portion, the selective drain portion and temperature adjustment portion connection, the control unit control
Reactive tank shaking table portion is made to the amplitude of fluctuation and duration of oscillation of the sample reactive tank, the selective feed liquor portion and described
The reagent in selective drain portion is added and the type of discharge and time, the temperature adjustment portion heat up to the sample reactive tank,
Cooling is kept for the time of constant temperature and the reaction time of temperature and the sample, to be automatically performed Sample pretreatment, sample
Denaturation hybridization and sample final wash.
Preferably, one or more modules are horizontally arranged in the full-automatic in situ hybridization device, and each module includes one
A or multiple sample reactive tanks, each sample reactive tank accommodate one or more sample hand baskets, load one in each sample hand basket
A or multiple samples, the sample hand basket is swung and swing in the horizontal direction with reactive tank shaking table portion, with abundant
Reaction or cleaning sample.
Preferably, reactive tank shaking table portion includes axis, and the axis is connect with the sample reactive tank and the control unit,
So that the control unit controls the axis movement, to drive the sample hand basket swing in the horizontal direction.
It preferably, further include temperature control sensor, temperature control sensor is used to detect the temperature of the sample reactive tank, and by institute
The temperature for stating sample reactive tank is transmitted to the temperature adjustment portion and the control unit.
Preferably, the full-automatic in situ hybridization device includes multiple module upper covers, and each module upper cover is used for will be described
Each module is sealed.
Preferably, the control unit is connected with the computer being located at outside the full-automatic in situ hybridization device by interface
It connects, the control unit is controlled by the computer.
Preferably, the computer carries out a key control to the full-automatic in situ hybridization device.
Preferably, the full-automatic in situ hybridization device passes through the reaction solution pumped by reagent or cleaning liquid described in
Selective feed liquor portion is pumped into the sample reactive tank, for carrying out sample process reaction and cleaning to the sample reactive tank, and
Waste reaction solution is pumped out in external waste liquid barrel from the selective drain portion.
Preferably, there is strainer in the pump.
Preferably, the sample reactive tank includes one or more reactive tank hand basket grooves, and each sample hand basket is located at one
In a reactive tank hand basket groove.
In view of problem above, for the full-automatic in situ hybridization device of the utility model according to experimental technique process, selection is appropriate
Reagent be added sample reactive tank, under working condition appropriate (temperature, time, swing), make sample reach process effect
Fruit is successively automatically performed complicated Sample pretreatment, denaturing samples hybridization, the big process flow of sample final wash three.This is practical
Novel is the efficient experimental provision of electromechanical integration, it can realize all kinds of hybridization in situ experiment whole process automations, standardization, height
Effectization.
Detailed description of the invention
Top view when Fig. 1 is the full-automatic in situ hybridization device opening upper cover of the utility model；
Fig. 2 is the cross-sectional view of the full-automatic in situ hybridization device of the utility model.
Specific embodiment
Below with reference to the accompanying drawings, the utility model is described in more detail, in order to which the utility model is more clear
It Chu and should be readily appreciated that.Those skilled in the art will recognize, in the spirit and scope without departing from the utility model
In the case of, described embodiment can be modified with a variety of different modes or combinations thereof.Therefore, attached drawing and description exist
Substantially be it is illustrative, be not intended to limit the scope of the claims.In addition, in the present specification, attached drawing is not pressed
Ratio is drawn, and identical appended drawing reference indicates identical part.
The embodiments of the present invention are described in detail below with reference to Fig. 1 and Fig. 2.The full-automatic in situ hybridization of the utility model
Device includes upper cover 1 and main body.Wherein, main body include sample reactive tank 22, reactive tank shaking table portion 29, selective feed liquor portion 26,
Selective drain portion 27, temperature adjustment portion 24 and control unit 31.Upper cover 1 provides sealing based on being used for.
Preferably, one or more modules 21 are horizontally arranged in main body, and each module 21 includes that one or more samples are anti-
Slot 22 is answered, each sample reactive tank 22 includes one or more sample hand baskets 23, loads one or more in each sample hand basket 23
A sample.However, this is only the preferred embodiment of the utility model, one or more samples can be set directly in main body
Reactive tank 22 or sample hand basket 23.
Reactive tank shaking table portion 29 is located at 22 lower section of sample reactive tank, for swinging sample reactive tank 22.It is hanging to be arranged in sample
Sample hand basket 23 in this reactive tank 22 is swung and swing in the horizontal direction with reactive tank shaking table portion 29, sufficiently to react
Or cleaning sample.Preferably, reactive tank shaking table portion 29 includes axis, and axis is connect with sample reactive tank 22 and control unit 31, so that control
31 control shaft of portion processed is mobile, to drive the swing in the horizontal direction of sample hand basket 23.
Each module 21 includes temperature adjustment portion 24 and temperature control sensor 25, and temperature control sensor 25 is for detecting sample reactive tank 22
Temperature, temperature adjustment portion 24 be used for sample reactive tank 22 carry out heating, cooling or keep constant temperature.Temperature adjustment portion 24 and temperature control sensor
25 can be set top, side or the bottom in each module 21 or sample reactive tank 22, only module 21 is arranged in Fig. 2
For side, and it is without being limited thereto.
Selective feed liquor portion 26 and selective drain portion 27 can be set in the upper of each module 21 or sample reactive tank 22
Portion, side or bottom, in Fig. 2 only with selective feed liquor portion 26 and selective drain portion 27 be separately positioned on module 21 top and
For bottom, and it is without being limited thereto.By pump by reagent reaction solution or cleaning liquid be pumped into selective feed liquor portion 26, for pair
Sample reactive tank 22 is cleaned, and the waste liquid after cleaning is pumped out in external waste liquid barrel from selective drain portion 27.It is excellent
Selection of land, pump is interior to have strainer.
Control unit 31 and sample reactive tank 22, reactive tank shaking table portion 29, selective feed liquor portion 26, selective drain portion 27 with
And temperature adjustment portion 24 connects, control unit 31 control reactive tank shaking table portion 29 to the amplitude of fluctuation and duration of oscillation of sample reactive tank 22,
Sample is reacted in the reagent addition in selective feed liquor portion 26 and selective drain portion 27 and the type of discharge and time, temperature adjustment portion 24
Slot 22 carries out heating, cooling or is kept for the time of constant temperature and the reaction time of temperature and sample, thus before being automatically performed sample
Processing, denaturing samples hybridization and sample final wash.
Preferably, control unit 31 is connect with the computer being located at outside full-automatic in situ hybridization device by interface, is passed through
Computer controls control unit 31, and without being limited thereto.Preferably, computer can carry out a key to full-automatic in situ hybridization device
Control, i.e., only by pressing the start key of full-automatic in situ hybridization device, computer can control control unit, with automatic
Sample pretreatment, denaturing samples hybridization and sample final wash are completed, without the intervention of very important person, and is encountering node
When, notice can be made a sound.
Preferably, full-automatic in situ hybridization device includes three modules 21, and each module 21 includes two sample reactive tanks
22, each sample reactive tank 22 accommodates a sample hand basket 23, loads two to four samples in each sample hand basket 23.
Preferably, which includes multiple module upper covers 28, and each module upper cover 28 is used for will be every
A module 21 is sealed.
Preferably, sample reactive tank 22 includes one or more reactive tank hand basket groove (not shown), each sample hand basket
23 are located in a reactive tank hand basket groove.
The in-situ hybridization method of the utility model includes the following steps:
Manually sample is placed in sample reactive tank；Following steps are executed automatically by control unit: passing through selectivity
Reagent is added to samples selection of the feed liquor portion into sample reactive tank, sample reactive tank is controlled in water by reactive tank shaking table portion
It square is swung up, the temperature of sample reactive tank is adjusted in real time by temperature adjustment portion, thus successively to this progress Sample pretreatment, sample
This denaturation hybridization, sample final wash, and the reagent after reaction is pumped out in external waste liquid barrel from selective drain portion.
The above is only the preferred embodiment of the present invention, is not intended to limit the utility model, for this field
Technical staff for, various modifications and changes may be made to the present invention.Within the spirit and principle of the utility model,
Any modification, equivalent replacement, improvement and so on should be included within the scope of protection of this utility model.
Claims (9)
1. a kind of full-automatic in situ hybridization device, comprising:
Sample reactive tank, for placing sample；
Reactive tank shaking table portion is located at below the sample reactive tank, for swinging the sample reactive tank；
Selective feed liquor portion and selective drain portion, the selectivity feed liquor portion and the selective drain portion and the sample are anti-
It answers slot to connect, reagent is added to samples selection of the selectivity feed liquor portion into the sample reactive tank, the examination after reaction
Agent is discharged from the selective drain portion；
Temperature adjustment portion carries out heating, cooling to the sample reactive tank or keeps constant temperature；And
Control unit, with the sample reactive tank, reactive tank shaking table portion, the selective feed liquor portion, the selective drain
Portion and temperature adjustment portion connection, the control unit control reactive tank shaking table portion to the amplitude of fluctuation of the sample reactive tank
With type and the time, institute of duration of oscillation, the reagent addition of the selective feed liquor portion and the selective drain portion and discharge
Temperature adjustment portion is stated to carry out heating, cooling to the sample reactive tank or kept for the time of constant temperature and the reaction of temperature and the sample
Time, to be automatically performed Sample pretreatment, denaturing samples hybridization and sample final wash.
2. full-automatic in situ hybridization device according to claim 1, wherein the full-automatic in situ hybridization device is horizontally
One or more modules are set, each module includes one or more sample reactive tanks, each sample reactive tank accommodate one or
Multiple sample hand baskets load one or more samples in each sample hand basket, and the sample hand basket is with reactive tank shaking table portion
It swings and swing in the horizontal direction, sufficiently to react or clean sample.
3. full-automatic in situ hybridization device according to claim 2, wherein reactive tank shaking table portion includes axis, described
Axis is connect with the sample reactive tank and the control unit, so that the control unit controls the axis movement, to drive the sample
The swing in the horizontal direction of this hand basket.
4. full-automatic in situ hybridization device according to claim 1 further includes temperature control sensor, temperature control sensor is for examining
The temperature of the sample reactive tank is surveyed, and the temperature of the sample reactive tank is transmitted to the temperature adjustment portion and the control unit.
5. full-automatic in situ hybridization device according to claim 2, the full-automatic in situ hybridization device includes multiple moulds
Block upper cover, each module upper cover is for each module to be sealed.
6. full-automatic in situ hybridization device according to claim 1, wherein the control unit be located at the full-automatic original
Computer outside the hybrid device of position is connected by interface, and the control unit is controlled by the computer.
7. full-automatic in situ hybridization device according to claim 6, wherein the computer is miscellaneous to the full-automatic original position
Device is handed over to carry out a key control.
8. full-automatic in situ hybridization device according to claim 1, wherein the full-automatic in situ hybridization device passes through pump
By in reagent reaction solution or cleaning liquid the sample reactive tank is pumped by the selective feed liquor portion, for the sample
This reactive tank carries out sample process reaction and cleaning, and waste reaction solution is pumped out to external waste liquid from the selective drain portion
In bucket, there is strainer in the pump.
9. in situ hybridization device according to claim 2, wherein the sample reactive tank includes one or more reactive tanks
Hand basket groove, each sample hand basket are located in a reactive tank hand basket groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820343172.2U CN208266182U (en) | 2018-03-13 | 2018-03-13 | Full-automatic in situ hybridization device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820343172.2U CN208266182U (en) | 2018-03-13 | 2018-03-13 | Full-automatic in situ hybridization device |
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| Publication Number | Publication Date |
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| CN208266182U true CN208266182U (en) | 2018-12-21 |
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| CN201820343172.2U Active CN208266182U (en) | 2018-03-13 | 2018-03-13 | Full-automatic in situ hybridization device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110272821A (en) * | 2018-03-13 | 2019-09-24 | 赵炜 | Full-automatic in situ hybridization device and method |
-
2018
- 2018-03-13 CN CN201820343172.2U patent/CN208266182U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110272821A (en) * | 2018-03-13 | 2019-09-24 | 赵炜 | Full-automatic in situ hybridization device and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20181225 Address after: 100085 Beijing Haidian District Shangdi Avon Garden Building 1 Gate 502 Patentee after: Zhao Wei Address before: 100142 Building A101, Zhongguancun Internet Cultural and Creative Industry Park, No. 1 Liangjiadian, Haidian District, Beijing Patentee before: BIOLANCET TECHNOLOGY Ltd. |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230905 Address after: 100142 Building A101, Zhongguancun Internet Cultural and Creative Industry Park, No. 1 Liangjiadian, Haidian District, Beijing Patentee after: BIOLANCET TECHNOLOGY Ltd. Address before: 100085 Beijing Haidian District Shangdi Avon Garden Building 1 Gate 502 Patentee before: Zhao Wei |