CN220364534U - Liquid path system of FISH single-tank pretreatment equipment - Google Patents

Liquid path system of FISH single-tank pretreatment equipment Download PDF

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Publication number
CN220364534U
CN220364534U CN202321847633.7U CN202321847633U CN220364534U CN 220364534 U CN220364534 U CN 220364534U CN 202321847633 U CN202321847633 U CN 202321847633U CN 220364534 U CN220364534 U CN 220364534U
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liquid
waste liquid
pipeline
communicated
reagent
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戢汇亮
肖永军
周世红
张忠雄
张俊
张宇
陈传坤
魏亮
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Wuhan Youzhiyou Medical Technology Co ltd
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Wuhan Youzhiyou Medical Technology Co ltd
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Abstract

The utility model relates to the technical field of medical in-vitro diagnostic instruments, in particular to a liquid path system of a FISH single-tank pretreatment device, which comprises a reagent selecting device, a liquid inlet and outlet device and a waste liquid collecting device, wherein the reagent selecting device, the liquid inlet and outlet device and the waste liquid collecting device are communicated through pipelines in sequence; the reagent selecting device comprises a plurality of reagent containers, each reagent container is communicated with the liquid inlet and outlet device through a pipeline respectively through the rotary valve, the rotary valve can effectively replace a method for installing a plurality of valves on one valve plate, the use and maintenance cost can be reduced, the condition that the whole valve plate fails and cannot be used due to the damage of one valve is avoided, the variable of the failure is reduced, the pipeline is enabled to be more stable and reliable, the internal device of the liquid path system is simple in structure and convenient to operate, the principle is clear and reliable, and the operation uniformity and the standardization of the FISH detection process can be effectively improved.

Description

Liquid path system of FISH single-tank pretreatment equipment
Technical Field
The utility model relates to the technical field of medical in-vitro diagnostic instruments, in particular to a liquid path system of a FISH single-tank pretreatment device.
Background
Conventional paraffin-embedded tissue sections (conventional sections) are the most widely used method in conventional slice making technology of histology, are commonly used for researching, observing and judging morphological changes of cell tissues in pathology, can be used for qualitative and positioning observation research of macromolecular substances such as polypeptides, proteins and the like of the cell tissues in the tissue sections in combination with immunological technology, can be used for gene positioning and state analysis in the cells in combination with a FISH method, and are widely used in research in the field of multidisciplinary at present.
In conventional FISH (fluorescence in situ hybridization) detection, paraffin-embedded tissue sections are subjected to dewaxing, penetrating by penetrating agents, pepsin digestion, washing, draining and the like. In the prior art, when a reagent is selected, valves with the same number as the reagent are usually required to be installed on a valve plate, and once a certain valve is abnormal, the whole equipment can be failed due to the fact that each valve has unstable variables, so that the valve cannot be used, and the experimental efficiency is affected.
Disclosure of Invention
The utility model provides a liquid path system of a FISH single-tank pretreatment device, which solves the technical problems that in the prior art, the FISH detection cannot unify standard operation specifications, so that experimental differences exist in detection and the requirements of traceable GLP experiments cannot be met.
(II) technical scheme
In order to solve the technical problems, the embodiment of the utility model provides a liquid path system of a FISH single-tank pretreatment device, which comprises a reagent selecting device, a liquid inlet and outlet device and a waste liquid collecting device, wherein the reagent selecting device, the liquid inlet and outlet device and the waste liquid collecting device are communicated through pipelines in sequence;
the reagent selecting device comprises a plurality of reagent containers and at least one rotary valve, wherein liquid outlets of the plurality of reagent containers are respectively communicated with the liquid inlet and outlet device through the rotary valve, and the liquid inlet and outlet device is communicated with the waste liquid collecting device through a pipeline.
Further, the rotary valve comprises a public end and a plurality of input ends, and the input ends are respectively communicated with the public end; each reagent container is respectively communicated with a single input end, and the common end is communicated with the liquid inlet and outlet device through a pipeline.
Further, the rotary valve further comprises an air hole site, and the air hole site is communicated with the public end.
Further, the liquid inlet and outlet device further comprises a reaction tank and a three-way valve;
the three-way valve is provided with a first interface, a second interface and a third interface, the first interface is communicated with the reaction tank through a pipeline, the second interface is communicated with the public end through a pipeline, and the third interface is communicated with the waste liquid collecting device through a pipeline.
Further, the liquid inlet and outlet device further comprises a peristaltic pump, and the peristaltic pump is connected to a pipeline between the first interface and the reaction tank.
Further, a communicating vessel is arranged on the side wall of the reaction tank, a first photoelectric sensor is arranged in the communicating vessel, and the first photoelectric sensor is used for detecting the liquid level height in the reaction tank.
Further, a second photoelectric sensor is arranged on a pipeline, wherein the pipeline is communicated with the waste liquid collecting device, of the third interface.
Further, the waste liquid collecting device comprises at least one waste liquid barrel, and the third interface is communicated with the waste liquid barrel through a pipeline.
Further, the waste liquid collecting device further comprises waste liquid valves in one-to-one correspondence with the waste liquid barrels, liquid discharge ports of the waste liquid valves are communicated with liquid inlets of the waste liquid barrels through pipelines, and liquid inlets of the waste liquid valves are communicated with the third interfaces through pipelines.
Further, the side of the reaction tank is also provided with an overflow hole, and the overflow hole is communicated with the waste liquid collecting device through a flow guide pipe.
The utility model has the beneficial effects that: the utility model provides a liquid path system of a FISH single-tank pretreatment device, which comprises a reagent selecting device, a liquid inlet and outlet device and a waste liquid collecting device, wherein the reagent selecting device, the liquid inlet and outlet device and the waste liquid collecting device are communicated through pipelines in sequence; the reagent selecting device comprises a plurality of reagent containers, each reagent container is communicated with the liquid inlet and outlet device through a pipeline through the rotary valve, the rotary valve can effectively replace a method for installing a plurality of valves on one valve plate, the use and maintenance cost can be reduced, the condition that the whole valve plate fails and cannot be used due to the damage of one valve is avoided, the variable of the failure is reduced, the pipeline is enabled to be more stable and reliable, the internal device of the liquid path system is simple in structure and convenient to operate, the principle is clear and reliable, the unified operation standardization of the FISH detection process can be effectively improved, and experimental differences are reduced to the greatest extent.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic diagram of a liquid path system of FISH single-tank pretreatment equipment according to an embodiment of the present utility model.
Icon: 1-a reagent selection device; 101-a reagent container; 102-rotating the valve; 1021-common; 1022-input; 1023-air hole site;
2-liquid inlet and outlet devices; 201-a reaction tank; 2011-communicating vessel; 2012-a first photosensor; 2013-spill orifice; 202-a three-way valve; 2021-first interface; 2022-second interface; 2023-third interface; 203-peristaltic pump;
3-a waste liquid collection device; 301-a second photosensor; 302-a waste liquid barrel; 303-waste liquid valve.
Detailed Description
The technical solutions of the present utility model will be clearly and completely described in connection with the embodiments, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be noted that the azimuth or positional relationship indicated by the terms "upper", "lower", etc. are based on the azimuth or positional relationship shown in the drawings, and are merely for convenience of describing the present utility model and simplifying the description, and are not indicative or implying that the apparatus or element in question must have a specific azimuth, be constructed and operated in a specific azimuth, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; the communication may be direct or indirect through an intermediate medium, or may be internal to two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art. Furthermore, in the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.
As shown in fig. 1, the utility model provides a liquid path system of FISH single-tank pretreatment equipment, which comprises a reagent selecting device 1, a liquid inlet and outlet device 2 and a waste liquid collecting device 3, wherein the reagent selecting device 1, the liquid inlet and outlet device 2 and the waste liquid collecting device 3 are communicated through pipelines in sequence; the reagent selecting device 1 comprises a plurality of reagent containers 101 and at least one rotary valve 102, wherein liquid outlets of the plurality of reagent containers 101 are respectively communicated with the liquid inlet and outlet device 2 through the rotary valve 102, and the liquid inlet and outlet device 2 is communicated with the waste liquid collecting device 3 through a pipeline.
In this embodiment, the liquid path system includes a reagent selecting device, a liquid inlet and outlet device, and a liquid waste collecting device, which are sequentially connected by a pipeline; the reagent selecting device comprises a plurality of reagent containers, each reagent container is communicated with the liquid inlet and outlet device through a pipeline through a rotary valve, the method of installing a plurality of valves on one valve plate can be effectively replaced by using one rotary valve, the use and maintenance cost can be reduced, the condition that the whole valve plate fails and cannot be used due to the damage of one valve is avoided, the fault variables are reduced, the pipeline is more stable and reliable, the internal device of the liquid path system is simple in structure and convenient to operate, the principle is clear and reliable, the operation uniformity normalization of the FISH detection process can be effectively improved, and experimental differences are reduced to the greatest extent.
Wherein, optionally, the rotary motion of the rotary valve 102 is driven by a motor, and the motor is driven instead of manual rotation, so that the fault variable is reduced, and the pipeline of the reagent selecting device 1 is more stable and reliable.
According to an embodiment of the present utility model, as shown in fig. 1, the rotary valve 102 includes a common terminal 1021 and a plurality of input terminals 1022, and the plurality of input terminals 1022 are respectively communicated with the common terminal 1021; each reagent vessel 101 is in communication with a separate input 1022, and the common port 1021 is in communication with the liquid inlet and outlet device 2 via a pipeline.
In this embodiment, a rotary valve 102 is disposed on a pipeline communicated between the reagent selecting device 1 and the liquid feeding and discharging device 2, preferably, an interface at the center of the rotary valve 102 is a common port 1021, an outer ring is an input port 1022, the input ports 1022 are disposed at intervals along the outer ring edge of the rotary valve 102, each individual input port 1022 is respectively connected with a reagent container 101, the middle common port 1021 and a reagent container 101 connected with the outer ring input port 1022 can be conducted by rotating a valve core of the rotary valve 102, and the common port 1021 and each input port 1022 are communicated through individual pipelines inside the rotary valve 102, so that reagent selection operation is completed, a common channel is included in a valve head of the rotary valve 102, and the common channel is short, so that the rotary valve 102 is very easy to clean after use, and not only is simple to operate, but also operation uniformity of a FISH detection process can be effectively improved.
Wherein the number of input ports 1022 is preferably equal to or greater than the number of reagent containers 101.
According to one embodiment of the present utility model, as shown in FIG. 1, the rotary valve 102 further includes an air port 1023, the air port 1023 being in communication with the common port 1021.
In this embodiment, the rotary valve 102 used in the reagent selecting apparatus 1 is provided with an air hole 1023 at the outer input end 1022 thereof, and when the common end 1021 of the rotary valve 102 is connected to the air hole 1023, the residual reagent in the common pipeline can be emptied, so that the residual reagent in the pipeline can be cleaned more easily. The main pipeline between the valve core and the outer ring channel in the valve head is very short by using the rotary valve 102, and the air hole site 1023 is arranged for exhausting the detection residual reagent in the main pipeline, so that the main pipeline is switched to the air hole site 1023 before the reagent container 101 is switched and selected, and then is switched to the next reagent container 101 after the main pipeline is exhausted, thereby effectively reducing the possibility of cross contamination of the detection reagent and reducing the detection experimental difference to a greater extent.
According to an embodiment of the present utility model, as shown in fig. 1, the liquid inlet and outlet device 2 further includes a reaction tank 201 and a three-way valve 202; the three-way valve 202 has a first port 2021, a second port 2022, and a third port 2023, the first port 2021 is in communication with the reaction tank 201 via a pipe, the second port 2022 is in communication with the common port 1021 via a pipe, and the third port 2023 is in communication with the waste liquid collecting device 3 via a pipe.
In this embodiment, the three-way valve 202 has a first interface 2021, a second interface 2022 and a third interface 2023, the first interface 2021 is in pipeline communication with the reaction tank 201, the second interface 2022 is in pipeline communication with the common end 1021, the third interface 2023 is in pipeline communication with the waste liquid collecting device 3, and by using one three-way valve 202, the liquid path direction of the single reaction tank 201 in this embodiment can be switched, so that the operation is simple and the principle is reliable.
According to an embodiment of the present utility model, as shown in fig. 1, the liquid inlet and outlet device 2 further includes a peristaltic pump 203, and the peristaltic pump 203 is connected to a pipeline between the first interface 2021 and the reaction tank 201.
In this embodiment, one end of the peristaltic pump 203 is connected to the first interface 2021 through a pipeline, the other end is connected to the reaction tank 201 through a pipeline, the first interface 2021 is connected to the peristaltic pump 203, the peristaltic pump 203 pumps the volume corresponding to the required reagent into the reaction tank 201, when the residual reagent detected in the reaction tank 201 needs to be discharged, the three-way valve 202 switches the direction, the peristaltic pump 203 reverses to discharge the residual reagent detected from the reaction tank 201, so that the purpose that the required reagent is heated, cooled, flowed, nursed, soaked and washed in the reaction tank 201 and then discharged is achieved. Different reagents enter the reaction tank 201 in different time periods, and then are subjected to temperature rising, temperature reducing, flowing, tending, soaking and sample flushing to complete a sample experiment flow.
In this embodiment, the pumping and discharging of the reagent in the reaction tank 201 are achieved by using a single peristaltic pump 203 to perform forward and reverse movements, and the forward and reverse rotations of the peristaltic pump 203 can relatively prolong the service life of the pipes in the peristaltic pump 203.
According to an embodiment of the present utility model, as shown in fig. 1, a communicating vessel 2011 is disposed on a side wall of a reaction tank 201, a first photoelectric sensor 2012 is installed in the communicating vessel 2011, and the first photoelectric sensor 2012 is used for detecting a liquid level in the reaction tank 201.
In this embodiment, the common end 1021 of the rotary valve 102 is connected to the three-way valve 202, the three-way valve 202 switches the reagent pipeline to the peristaltic pump 203, the peristaltic pump 203 pumps the reagent into the reaction tank 201 by rotation, the first photoelectric sensor 2012 on the communicating vessel 2011 feeds back whether the pumped reagent reaches a predetermined capacity, and when the liquid level in the reaction tank 201 reaches a predetermined height, the first photoelectric sensor 2012 on the communicating vessel 2011 triggers to stop pumping the reagent.
Preferably, the first photoelectric sensor 2012 is further provided with a controller, and the controller is configured to receive a liquid level height signal detected by the first photoelectric sensor 2012, compare the detected liquid level height signal T with a preset liquid level height signal threshold T1, and when T is greater than or equal to T1, the controller controls the peristaltic pump 203 to stop pumping the reagent into the reaction tank 201.
The liquid path system provided in this embodiment uses the combination of the communicating vessel 2011 and the first photoelectric sensor 2012 to achieve the quantitative of reagent pumping, that is, the purpose of emptying the reagent in the waste liquid pipeline, and the liquid path principle is simple and reliable.
According to an embodiment of the present utility model, as shown in fig. 1, a second photoelectric sensor 301 is disposed on a pipeline where the third interface 2023 communicates with the waste liquid collecting device 3.
In this embodiment, the second photoelectric sensor 301 is disposed on the pipeline of the third interface 2023 communicating with the waste liquid collecting apparatus 3, and when the three-way valve 202 is switched, the peristaltic pump 203 is reversed to discharge the residual reagent detected in the reaction tank 201, so that it can be determined whether the waste liquid of the residual reagent detected in the reaction tank 201 is discharged or not by the second photoelectric sensor 301 on the pipeline of the waste liquid collecting apparatus 3.
According to one embodiment of the present utility model, as shown in fig. 1, the waste liquid collecting device 3 includes at least one waste liquid tank 302, and the third port 2023 is in communication with the waste liquid tank 302 through a pipeline.
In this embodiment, the waste liquid collecting device 3 includes at least one waste liquid tank 302, and the waste liquid tank 302 is connected to the third port 2023 through a pipeline to store the waste liquid of the detection residual reagent discharged from the third port 2023.
According to an embodiment of the present utility model, as shown in fig. 1, the waste liquid collecting device 3 further includes waste liquid valves 303 corresponding to the waste liquid barrels 302 one by one, the liquid outlet of the waste liquid valves 303 is communicated with the liquid inlet of the waste liquid barrels 302 through a pipeline, and the liquid inlet of the waste liquid valves 303 is communicated with the third interface 2023 through a pipeline.
In this embodiment, the waste liquid valves 303 are in one-to-one correspondence with the waste liquid barrels 302, and the waste liquid valves 303 control the waste liquid of the detection residual reagent in the reaction tank 201 to be discharged into the waste liquid barrels 302.
According to an embodiment of the present utility model, as shown in fig. 1, the side surface of the reaction tank 201 is further provided with an overflow hole 2013, and the overflow hole 2013 is communicated with the waste liquid collecting device 3 through a flow guiding pipe.
In this embodiment, the side of the reaction tank 201 is provided with the overflow hole 2013, when the equipment fails, the reagent in the reaction tank 201 automatically flows out to the waste liquid collecting device 3 through the overflow hole 2013 due to gravity, so that the risk of circuit burnout caused by reagent overflow is avoided, and the use safety of the liquid path system equipment provided by this embodiment is improved. When the liquid inlet fails, the overflow hole 2013 arranged on the side surface of the reaction tank 201 can automatically discharge the liquid from the overflow hole 2013 and flow into the waste liquid collecting device 3, so that serious consequences caused by the flowing to a circuit part are avoided.
The foregoing is only illustrative of the present utility model and is not to be construed as limiting thereof, but rather as various modifications, equivalent arrangements, improvements, etc., within the spirit and principles of the present utility model.

Claims (10)

1. The liquid path system of the FISH single-tank pretreatment equipment is characterized by comprising a reagent selecting device (1), a liquid inlet and outlet device (2) and a waste liquid collecting device (3), wherein the reagent selecting device (1), the liquid inlet and outlet device (2) and the waste liquid collecting device (3) are communicated through pipelines in sequence;
the reagent selecting device (1) comprises a plurality of reagent containers (101) and at least one rotary valve (102), wherein liquid outlets of the plurality of reagent containers (101) are respectively communicated with the liquid inlet and outlet device (2) through the rotary valve (102), and the liquid inlet and outlet device (2) is communicated with the waste liquid collecting device (3) through a pipeline.
2. A liquid circuit system of FISH single tank pretreatment equipment according to claim 1, characterized in that said rotary valve (102) comprises a common terminal (1021) and a plurality of input terminals (1022), said plurality of input terminals (1022) being respectively in communication with said common terminal (1021); each reagent container (101) is respectively communicated with a single input end (1022), and the common end (1021) is communicated with the liquid inlet and outlet device (2) through a pipeline.
3. A fluid circuit system of a FISH single tank pretreatment device according to claim 2, wherein said rotary valve (102) further comprises an air hole site (1023), said air hole site (1023) being in communication with said common port (1021).
4. The liquid path system of FISH single-tank pretreatment equipment according to claim 2, wherein the liquid inlet and outlet device (2) further comprises a reaction tank (201) and a three-way valve (202);
the three-way valve (202) is provided with a first interface (2021), a second interface (2022) and a third interface (2023), the first interface (2021) is communicated with the reaction tank (201) through a pipeline, the second interface (2022) is communicated with the public end (1021) through a pipeline, and the third interface (2023) is communicated with the waste liquid collecting device (3) through a pipeline.
5. The liquid path system of FISH single tank pretreatment equipment according to claim 4, wherein said liquid inlet and outlet device (2) further comprises a peristaltic pump (203), and said peristaltic pump (203) is connected to a pipeline between said first interface (2021) and said reaction tank (201).
6. The liquid path system of FISH single tank pretreatment equipment according to claim 5, wherein a communicating vessel (2011) is provided on a side wall of the reaction tank (201), a first photoelectric sensor (2012) is installed in the communicating vessel (2011), and the first photoelectric sensor (2012) is used for detecting a liquid level height in the reaction tank (201).
7. The liquid path system of FISH single tank pretreatment apparatus according to claim 5, wherein a second photoelectric sensor (301) is provided on a pipeline where the third interface (2023) communicates with the waste liquid collecting device (3).
8. The fluid circuit system of FISH single-tank pretreatment equipment according to claim 4, wherein said waste liquid collecting device (3) comprises at least one waste liquid tank (302), and said third port (2023) is in communication with said waste liquid tank (302) through a pipeline.
9. The liquid path system of FISH single tank pretreatment equipment according to claim 8, wherein said waste liquid collecting device (3) further comprises waste liquid valves (303) corresponding to said waste liquid barrels (302) one by one, a liquid outlet of said waste liquid valves (303) is communicated with a liquid inlet of said waste liquid barrels (302) through a pipeline, and a liquid inlet of said waste liquid valves (303) is communicated with said third interface (2023) through a pipeline.
10. The liquid path system of FISH single tank pretreatment equipment according to claim 4, wherein the side surface of the reaction tank (201) is further provided with an overflow hole (2013), and the overflow hole (2013) is communicated with the waste liquid collecting device (3) through a flow guiding pipe.
CN202321847633.7U 2023-07-13 2023-07-13 Liquid path system of FISH single-tank pretreatment equipment Active CN220364534U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202321847633.7U CN220364534U (en) 2023-07-13 2023-07-13 Liquid path system of FISH single-tank pretreatment equipment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202321847633.7U CN220364534U (en) 2023-07-13 2023-07-13 Liquid path system of FISH single-tank pretreatment equipment

Publications (1)

Publication Number Publication Date
CN220364534U true CN220364534U (en) 2024-01-19

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CN202321847633.7U Active CN220364534U (en) 2023-07-13 2023-07-13 Liquid path system of FISH single-tank pretreatment equipment

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