WO2019000460A1 - Sample analyzer and driving method therefor - Google Patents

Abstract

A sample analyzer (100) and a driving method thereof, the sample analyzer (100) comprising an air pump (1), an air tank group (2), a sampling component (3), a reaction component (4), and a detection component (5). The air pump (1) is used for establishing a positive pressure and a negative pressure in the air tank group (2), and the positive pressure and the negative pressure are used for: driving the sampling component (3) to collect a biological sample; and/or driving the reaction component (4) to process the biological sample to form a solution to be tested, the reaction component (4) comprising at least a reaction tank; and/or driving the solution to be tested to be tested by the detection component (5) to obtain a test signal. The sample analyzer (100) is low in costs.

Description

Sample analyzer and driving method thereof Technical field

The present invention relates to the field of medical device technology, and in particular, to a sample analyzer and a method for driving the sample analyzer.

Background technique

Existing sample analyzers require a variety of different drive pressures to provide different drive pressures for multiple lines in a sample analyzer. The drive pressure typically includes at least two positive pressures and at least two negative pressures. The sample analyzer can establish both positive and negative pressures by using a large-flow, large-volume double-head air pump. The positive pressure output establishes the highest positive pressure through the pressure relief valve, and the other relatively low positive pressure uses different pressure regulating valves to regulate the output; the negative pressure output establishes the vacuum with the highest vacuum through the restrictor tube, and the other vacuum with lower vacuum The output is adjusted with a relief valve. However, the driving pressure in the sample analyzer of the above scheme always needs to be driven by the double-head air pump, and the cost is high.

Summary of the invention

The technical problem to be solved by the present invention is to provide a lower cost sample analyzer and a sample analyzer driving method.

In order to achieve the above object, the embodiments of the present invention adopt the following technical solutions:

In one aspect, a sample analyzer is provided, including an air pump, a gas storage tank set, a sampling assembly, a reaction assembly, and a detection assembly, the air pump for establishing a positive pressure and a negative pressure in the gas storage tank group, the positive The pressure and the negative pressure are used to:

Driving the sampling component to collect a biological sample;

And/or driving the reaction assembly to process the biological sample to form a test solution, the reaction assembly comprising at least one reaction cell;

And/or driving the test solution to be detected by the detecting component to obtain a detection signal.

Wherein the gas storage tank set includes a first gas storage tank and a second gas storage tank, the gas pump is connected to the first gas storage tank through a first control valve for establishing in the first gas storage tank a first positive pressure, the air pump is connected to the second gas storage tank through a second control valve for establishing a first negative in the second gas storage tank Pressure.

Wherein the air pump is a single-head pump for establishing pressure on the first gas storage tank when the first control valve is turned on and the second control valve is cut off, and is cut off at the first control valve And the second gas storage tank is pressurized when the second control valve is turned on.

Wherein the air pump is a single-head pump or a double-headed pump for the first gas storage tank and the second gas storage when the first control valve is turned on and the second control valve is turned on The tank is built to pressure.

Wherein the sample analyzer further comprises a controller and a pressure sensor group, the pressure sensor group is configured to detect a pressure in the gas tank group and feed back a signal to the controller, the controller according to the signal The actions of the air pump, the first control valve, and the second control valve are controlled.

Wherein, at least one pressure breaking valve is disposed on the flow path of the sample analyzer, and the first positive pressure is used to drive the pressure breaking valve.

Wherein, the sample analyzer further comprises a waste liquid pool connected to the second gas storage tank, and a liquid pump for extracting waste liquid in the waste liquid pool.

Wherein, the waste liquid pool is provided with a first float switch for detecting the liquid level in the waste liquid pool.

Wherein, the sample analyzer further comprises a buffer pool connected between the second gas storage tank and the waste liquid pool, wherein the buffer pool is used to prevent waste liquid in the waste liquid pool from being poured into the waste liquid pool The second gas storage tank.

The second air tank is provided with a second float switch for detecting the liquid level in the second air tank.

Wherein, the sample analyzer further comprises a waste liquid pool and a liquid pump, wherein the liquid pump is used for extracting waste liquid in the waste liquid pool and establishing a negative pressure in the waste liquid pool.

Wherein the waste liquid pool is connected to the reaction assembly, and the waste liquid pool is used to collect the waste liquid of the reaction assembly.

Wherein the gas storage tank group further includes a third gas storage tank, wherein the first gas storage tank is connected to the third gas storage tank through a third control valve for passing the first positive pressure in the third storage tank A second positive pressure is established in the gas cylinder.

Wherein the sample analyzer further includes a sixth control valve and a first restrictor, the sixth control valve being connected between the third air tank and the first restrictor, the first A flow restrictor is used to release a portion of the pressure within the third gas storage tank.

Wherein the sample analyzer further comprises a sheath liquid pool and an flow chamber, an outlet of the sheath liquid pool is connected to a sheath liquid inlet of the flow chamber, and the third gas storage tank is connected to the sheath liquid pool for pushing The sheath fluid in the sheath fluid pool flows into the flow chamber.

Wherein the controller couples the third control valve for disconnecting the third gas storage tank through the third control valve when the sheath liquid in the sheath liquid pool flows into the flow chamber The first gas storage tank.

Wherein the pressure sensor group further includes a third pressure sensor, wherein the third pressure sensor is configured to open the first gas storage tank and the third gas storage tank at the third control valve When the sheath liquid in the sheath liquid pool flows toward the flow chamber, the pressure in the third gas tank and/or the sheath liquid pool is detected.

Wherein the gas storage tank set further includes a fourth gas storage tank, wherein the first gas storage tank is connected to the fourth gas storage tank through a fourth control valve for passing the first positive pressure in the fourth storage tank A third positive pressure is established in the gas cylinder.

Wherein the sample analyzer comprises a liquid storage tank connected to the first reaction tank, and the fourth gas storage tank is connected to the liquid storage tank for using the storage tank The reagent in the bath is pushed into the first reaction cell.

Wherein the sample analyzer further comprises a metering pump having a diaphragm and a liquid chamber and a gas chamber on both sides of the diaphragm, the metering pump connecting the gas tank group, the liquid chamber communicating with the liquid chamber In the gas storage tank group, the positive pressure pushes the diaphragm to move in the direction of the gas chamber, and when the gas chamber communicates with the gas storage tank group, the positive pressure pushes the diaphragm toward the liquid chamber Move in direction.

Wherein the sample analyzer comprises a liquid storage tank and a first reaction tank, and the liquid chamber is connected between the liquid storage tank and the first reaction tank.

Wherein the gas storage tank group further includes a fifth gas storage tank, wherein the second gas storage tank is connected to the fifth gas storage tank through a fifth control valve for passing the first negative pressure in the first A second negative pressure is established in the five gas storage tanks.

Wherein the sample analyzer further includes a seventh control valve and a second restrictor, the seventh control valve being connected between the fifth gas tank and the second restrictor, the second A flow restrictor is used to release a portion of the pressure within the fifth gas storage tank.

Wherein, the sample analyzer further comprises a second reaction tank, and the fifth gas storage tank is connected to an outlet of the second reaction tank.

In another aspect, a method of driving a sample analyzer is provided, the driving method comprising:

Driving the air pump to establish positive and negative pressures in the gas storage tank group; and

The positive pressure and the negative pressure drive the flow path of the sample analyzer.

Wherein, the “driving air pump establishes positive pressure and negative pressure in the gas storage tank group” includes:

The air pump is driven to establish a first positive pressure in the first gas storage tank and a first negative pressure in the second gas storage tank.

Wherein, when the absolute value of the pressure of the first positive pressure is less than the first threshold, the air pump is driven to establish a pressure in the first gas storage tank, so that the absolute value of the pressure of the first positive pressure reaches the first Threshold.

Wherein, when the absolute value of the first positive pressure is greater than or equal to the first threshold and the absolute value of the first negative pressure is less than the second threshold, driving the air pump to build pressure in the second gas storage tank, The absolute value of the pressure of the first negative pressure is brought to the second threshold.

Wherein, when the absolute value of the first positive pressure is greater than or equal to the first threshold and less than the third threshold, and the absolute value of the first negative pressure is greater than or equal to the second threshold, driving the air pump in the first storage A pressure is built in the gas cylinder such that the absolute value of the first positive pressure reaches the third threshold.

Wherein the absolute value of the first positive pressure is greater than or equal to a third threshold, and the absolute value of the first negative pressure is greater than or equal to a second threshold and less than a fourth threshold, driving the air pump in the second storage A pressure is built in the gas tank such that the absolute value of the first negative pressure reaches the fourth threshold.

Wherein, when the absolute value of the first positive pressure is greater than or equal to the third threshold and less than the fifth threshold, and the absolute value of the first negative pressure is greater than or equal to the fourth threshold, driving the air pump in the first storage A pressure is built in the gas cylinder such that the absolute value of the first positive pressure reaches the fifth threshold.

Wherein the first positive pressure establishes a second positive pressure in the third gas storage tank.

Wherein the second positive pressure pushes the sheath fluid in the sheath liquid pool into the flow chamber.

Wherein, the third gas storage tank and the first gas storage tank are disconnected before the second positive pressure pushes the sheath liquid in the sheath liquid pool into the flow chamber.

Wherein, when the second positive pressure pushes the sheath liquid in the sheath liquid pool into the flow chamber, the pressure change of the second positive pressure is detected by the third pressure sensor.

Wherein the first positive pressure establishes a third positive pressure in the fourth gas storage tank.

Wherein the liquid storage tank is connected to the first reaction tank, and the fourth gas storage tank is connected to the liquid storage tank to provide a driving force for the reagents in the liquid storage tank to enter the first reaction tank.

Wherein the first negative pressure establishes a second negative pressure in the fifth gas storage tank.

Wherein, the fifth gas storage tank is turned to the outlet of the second reaction tank to extract the liquid in the second reaction tank by the second negative pressure.

The process of driving the air pump to establish a first positive pressure in the first gas storage tank includes:

The air pump establishes a first positive pressure in the first gas storage tank whose absolute value is greater than a first preset value, and turns on the first gas storage tank to the atmosphere, so that the pressure of the first positive pressure is absolutely Decreasing the value to the first preset value;

and / or,

The process of driving the air pump to establish a first negative pressure in the second gas storage tank includes:

The air pump establishes a first negative pressure in the second gas storage tank whose absolute value is greater than a second preset value, and turns on the second gas storage tank to the atmosphere, so that the pressure of the second negative pressure is absolutely The value is lowered to the second preset value;

and / or,

The process of establishing the second positive pressure in the third gas storage tank by the first positive pressure comprises:

Turning on the first gas storage tank and the third gas storage tank, so that the first positive pressure is built in the third gas storage tank to form a pressure absolute value greater than a third preset value a second positive pressure; conducting the third gas storage tank to the atmosphere, reducing the absolute value of the second positive pressure to the third predetermined value;

and / or,

The process of establishing the second negative pressure in the fifth gas storage tank by the first negative pressure comprises:

Turning on the fifth gas storage tank and the second gas storage tank, so that the first negative pressure is built in the fifth gas storage tank to form a pressure absolute value greater than a fourth preset value Two negative pressures; conducting the fifth gas storage tank to the atmosphere, and decreasing the absolute value of the second negative pressure to the fourth predetermined value.

Wherein the metering pump of the sample analyzer comprises a liquid chamber and a gas chamber, the liquid chamber is connected to the liquid storage tank and the first reaction tank, and the driving method further comprises:

The liquid storage tank communicates with the positive pressure to push liquid in the liquid storage tank into the liquid chamber by using the positive pressure; and

The plenum communicates the positive pressure to push liquid in the liquid chamber toward the first reaction chamber using the positive pressure.

Compared with the prior art, the present invention has the following beneficial effects:

The sample analyzer establishes a positive pressure and a negative pressure in the gas storage tank group through the air pump, and then passes through The positive pressure and the negative pressure in the gas cylinder group serve as the main driving force of the sample analyzer, thereby being able to replace the large-flow air pump in the prior art, reducing the cost and energy consumption of the sample analyzer. At the same time, since the sample analyzer can employ a small volume of the air pump, the overall volume of the sample analyzer can be reduced.

DRAWINGS

In order to more clearly illustrate the technical solutions of the present invention, the drawings used in the embodiments will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the present invention, which are common in the art. For the skilled person, other drawings can be obtained as shown in these drawings without any creative work.

1 is a schematic block diagram of a sample analyzer provided by the present invention.

2 is a partial structural schematic view of the sample analyzer shown in FIG. 1.

3 is a schematic view showing the structure of another part of the sample analyzer shown in FIG. 1.

4 is a schematic structural view of still another part of the sample analyzer shown in FIG. 1.

FIG. 5 is a schematic structural view of still another part of the sample analyzer shown in FIG. 1. FIG.

Fig. 6 is a schematic view showing the structure of the sample analyzer shown in Fig. 1.

Fig. 7 is a structural schematic view showing still another part of the sample analyzer shown in Fig. 1.

Figure 8 is a graph showing the pressure variation in the third gas tank of the sample analyzer shown in Figure 1.

Detailed ways

The technical solutions in the embodiments of the present invention are clearly and completely described in the following with reference to the accompanying drawings in the embodiments of the present invention. It is obvious that the described embodiments are only a part of the embodiments of the present invention, but not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative efforts are within the scope of the present invention.

Referring to FIG. 1, an embodiment of the present invention provides a sample analyzer 100. The sample analyzer 100 can be used to perform biological sample analysis, which can be blood, urine, and the like.

The sample analyzer 100 includes an air pump 1, a gas storage tank set 2, a sampling assembly 3, a reaction assembly 4, and a detection assembly 5. The air pump 1 is used to establish a positive pressure and a negative pressure in the gas tank group 2. The positive pressure and the negative pressure are used to: drive the sampling component 3 to collect a biological sample; and/or drive the The reaction assembly 4 processes the biological sample to form a test solution, the reaction assembly 4 includes at least one reaction cell; and/or drives the test solution to be detected by the detection assembly 5 to obtain a detection signal.

In the present embodiment, the sample analyzer 100 establishes a positive pressure and a negative pressure in the gas tank group 2 by the air pump 1, and then passes the positive pressure and the negative pressure in the gas tank group 2 as The main driving force of the sample analyzer 100, thereby being able to replace the large-flow air pump 1 of the prior art, reduces the cost and energy consumption of the sample analyzer 100. Meanwhile, since the sample analyzer 100 can employ the small volume of the air pump 1, the overall volume of the sample analyzer 100 can be reduced.

It will be appreciated that the air pump 1 and the gas cylinder stack 2 can form part of a drive assembly of the sample analyzer 100. The drive assembly can be used to drive various flow paths (including gas and liquid paths) and devices in the sample analyzer 100. The sample analyzer 100 also includes a waste treatment assembly for collecting and discharging waste liquid in the sample analyzer 100. The sampling component 3 can include a sampler for acquiring and distributing biological samples.

Referring to FIG. 1 and FIG. 2 together, as an alternative embodiment, the gas storage tank set 2 includes a first gas storage tank 21 and a second gas storage tank 22. The air pump 1 is connected to the first air tank 21 through a first control valve 23 for establishing a first positive pressure in the first air tank 21. The first positive pressure is used to provide a primary positive pressure driving force to the sample analyzer 100. The first control valve 23 is for connecting or shutting off the air pump 1 and the first air tank 21 . The air pump 1 is connected to the second air tank 22 through a second control valve 24 for establishing a first negative pressure in the second air tank 22. The first negative pressure is used to provide a primary negative pressure driving force to the sample analyzer 100. The second control valve 24 is for connecting or shutting off the air pump 1 and the second air tank 22.

In an embodiment, the air pump 1 is a single-head pump for establishing pressure on the first gas storage tank 21 when the first control valve 23 is turned on and the second control valve 24 is turned off. And the second air reservoir 22 is pressurized when the first control valve 23 is cut off and the second control valve 24 is turned on.

In the embodiment, the air pump 1 is a one-way pressure-controlled single-head pump, and pressure is newly established for the first gas storage tank 21 or separately for the second gas storage tank 22 at the same time. The one-head pump that is unidirectionally built is very low cost, which is advantageous for further reducing the cost of the sample analyzer 100.

It can be understood by those skilled in the art that the air pump 1 can also be a single-head pump or a double-head pump for the first when the first control valve 23 is turned on and the second control valve 24 is turned on. The gas storage tank 21 and the second gas storage tank 22 are pressurized. In this embodiment, the air pump 1 is capable of achieving two-way pressure build-up. The single-head pump or the double-headed pump can simultaneously pressurize the first gas storage tank 21 and the second gas storage tank 22 at the same time, thereby increasing the pressure-building speed of the sample analyzer 100, which is advantageous for The detection speed of the sample analyzer 100 is increased. Of course, the air pump 1 that is bi-directionally built may also separately pressurize the first gas storage tank 21 or separately pressurize the second gas storage tank 22 at the same time.

Referring to FIG. 1 and FIG. 2 together, as an alternative embodiment, the sample analyzer 100 further includes a controller 6 and a pressure sensor group 7. The pressure sensor group 7 is for detecting the pressure in the gas tank group 2 and feeding back a signal to the controller 6. The controller 6 controls the actions of the air pump 1, the first control valve 23, and the second control valve 24 in accordance with the signal.

The pressure sensor group 7 includes a plurality of pressure sensors, which are respectively disposed in a plurality of air tanks of the gas tank group 2, for example, disposed in the first gas storage tank 21. A first pressure sensor 71 and a second pressure sensor 72 disposed in the second air reservoir 22. The plurality of pressure sensors can monitor the pressures in the respective plurality of gas storage tanks detected in real time.

The controller 6 is used to control both the air pump 1, the first control valve 23 and the second control valve 24, as well as to control other components in the sample analyzer 100. The controller 6 is capable of controlling the workflow of the sample analyzer 100 and processing the detection signals to form an analysis result.

For example, the first pressure sensor 71 monitors the pressure of the first positive pressure in the first gas storage tank 21 in real time, and when the first positive pressure is insufficient, the controller is required to build pressure. 6 controlling the first control valve 23 to communicate with the air pump 1 and the first gas storage tank 21, the air pump 1 is operated, and the air pump 1 builds pressure in the first gas storage tank 21 to make the The pressure of the first positive pressure rises. After the first pressure sensor 71 detects that the pressure of the first positive pressure reaches a demand, the controller 6 controls the first control valve 23 to cut off the air pump 1 and the first gas storage tank 21, The air pump 1 is stopped.

The process of establishing pressure in the first gas storage tank 21 is: the air pump 1 first establishes a first positive pressure in the first gas storage tank 21 that the absolute value of the pressure is greater than a first preset value. . The first gas storage tank 21 is then turned on to the atmosphere to lower the absolute value of the first positive pressure to the first predetermined value. Since the initial pressure value of the first positive pressure established by the air pump 1 in the first gas storage tank 21 is greater than the first preset value, the first first occurs even if an overshoot and rebound phenomenon occurs. The pressure value of the positive pressure can still maintain a state greater than the first preset value, and then the absolute value of the pressure of the first positive pressure is lowered to the first preset value by releasing a portion of the first positive pressure. After the completion of the pressure building process, The first positive pressure has an accurate pressure value. In short, the above-mentioned pressure-building process can eliminate the phenomenon of overshoot and rebound and achieve accurate pressure build-up.

The second pressure sensor 72 monitors the pressure of the first negative pressure in the second gas storage tank 22 in real time, and when the first negative pressure is insufficient to establish pressure, the controller 6 controls the The second control valve 24 communicates with the air pump 1 and the second gas storage tank 22, the air pump 1 operates, and the air pump 1 builds pressure in the second gas storage tank 22 to make the first negative pressure The pressure is falling. After the second pressure sensor 72 detects that the pressure of the first negative pressure reaches a demand, the controller 6 controls the second control valve 24 to cut off the air pump 1 and the second gas storage tank 22, The air pump 1 is stopped.

The process of establishing the pressure in the second gas storage tank 22 is: the air pump 1 first establishes a first negative pressure in the second gas storage tank 22 that the absolute value of the pressure is greater than a second preset value. . The second gas storage tank 22 is then turned on to the atmosphere to lower the absolute value of the first negative pressure to the second predetermined value. The pressure-building process eliminates both overshoot and rebound and achieves accurate pressure build-up.

Referring to FIG. 1 and FIG. 2 together, as an alternative embodiment, at least one pressure-cut valve 8 is provided on the flow path of the sample analyzer 100, and the first positive pressure is used to drive the pressure-cut valve. 8. The pressure shut-off valve 8 can be driven by air pressure, for example the first positive pressure, at which time the pressure-off valve 8 is connected to the first gas storage tank 21.

In the present embodiment, since the internal passage of the pressure-actuated valve 8 driven by the air pressure is relatively smooth, when the pressure-cut valve 8 is disposed in the pipe, contamination of the fluid in the pipe can be reduced.

In one embodiment, the pressure-cutting valve 8 can be disposed between the reaction assembly 4 and the detection assembly 5, and the liquid to be tested formed by the reaction assembly 4 passes through the pressure-cut valve 8 to enter the detection. The component 5, the pressure-cutting valve 8 is capable of reducing contamination of the liquid to be tested, thereby ensuring the accuracy of the detection result of the sample analyzer 100.

In another embodiment, the pressure relief valve 8 can be disposed on a line in the waste treatment assembly. Since the fluid flowing in the pipeline in the waste liquid treatment assembly has a large amount of impurities, the common valve member is easily blocked by the accumulation of impurities, and the service life is short. The internal pressure passage of the pressure relief valve 8 in the present embodiment is smooth. It can reduce the risk of blockage by impurities and has a long service life.

Referring to FIG. 1 and FIG. 2 together, as an alternative embodiment, the sample analyzer 100 further includes a waste liquid tank 91 and a liquid pump 92. The waste liquid tank 91 is connected to the second gas storage tank 22 for extracting waste liquid in the waste liquid tank 91 and establishing a negative pressure in the waste liquid tank 91. Waste liquid Pool 91 and the liquid pump 92 are part of the waste treatment assembly.

In this embodiment, when the second gas storage tank 22 communicates with the waste liquid pool 91, a negative pressure environment is established in the waste liquid pool 91 by using the first negative pressure, and the waste liquid pool 91 passes. The internal negative pressure extracts the waste liquid in the sample analyzer 100 to achieve waste collection. Since the waste liquid in the waste liquid tank 91 is discharged to the outside of the machine through the liquid pump 92 for discharge, it is not necessary to switch the pressure in the waste liquid tank 91, and the waste liquid pool 91 can always maintain a negative pressure state. So that the waste liquid pool 91 can continuously extract the waste liquid in the sample analyzer 100 through its internal negative pressure, so that the waste liquid action and the discharge waste liquid action of the waste liquid processing assembly can be performed in parallel and mutually The waste liquid treatment efficiency of the waste liquid processing assembly is high without interference, and the whole machine measurement speed of the sample analyzer 100 is fast. The negative pressure is always maintained in the waste liquid tank 91, and there is no need to perform positive and negative pressure switching, so that the increase of the air consumption caused by the switching of the positive and negative pressures can be avoided, and the air pump 1 which is advantageous for the small flow rate can better satisfy the said The drive requirements of the sample analyzer 100. Since the liquid pump 92 can discharge the waste liquid in the waste liquid pool 91 in time, it is possible to reduce the waste liquid or air bubbles in the waste liquid pool 91 from flowing into the second gas storage tank 22 or the air pump. The risk of 1 enables the sample analyzer 100 to function properly for a long time.

It can be understood that although the liquid pump 92 is used to discharge the waste liquid and the auxiliary pressure can effectively reduce the probability of waste liquid backflow, various device components may fail or the waste liquid pipe may be clogged, so the sample analyzer 100 An anti-backflow device has been added to further reduce the risk of waste backflow.

In the first embodiment, the waste liquid pool 91 is provided with a first float switch 911 for detecting the liquid level in the waste liquid tank 91. When the first float switch 911 floats up, the sensor mounted on the first float switch 911 detects a change in potential, indicating that the first float switch 911 in the waste liquid pool 91 has floated. If the time of continuous floating exceeds the set value, the alarm stops measuring, thereby preventing the waste liquid from being poured into the second gas storage tank 22. Those skilled in the art will appreciate that in this embodiment, the first float switch 911 is in a continuous detection state. In other embodiments, the intermittent detection mode may also be adopted at the first float switch 911, for example, detecting whether the first float switch 911 is floating at a preset time point, and if it is floating, the alarm stops measuring.

In a second embodiment, the sample analyzer 100 further includes a buffer pool 93. The buffer pool 93 is connected between the second gas storage tank 22 and the waste liquid pool 91, and the buffer pool 93 is configured to prevent waste liquid in the waste liquid pool 91 from being poured into the second storage tank. Gas tank 22. The buffer pool 93 can be a reservoir having a low inlet and a high outlet.

In the third embodiment, the second air reservoir 22 is provided with a second float switch 221 for detecting the liquid level in the second air tank 22. When the second float switch 221 floats up, the sensor mounted on the second float switch 221 of the book detects a change in potential, that is, if liquid has entered in the second gas storage tank 22, an alarm is immediately issued. Stop the measurement. Similarly, a float switch for measuring the level of the liquid level can be provided in other gas storage tanks and/or reservoirs and/or waste liquid pools.

It can be understood that the above three embodiments can also be combined with each other to form a more effective anti-backflow device.

Optionally, a shut-off valve is disposed between the waste liquid pool 91 and the second gas storage tank 22 for connecting or cutting the waste liquid pool 91 and the second gas storage tank 22.

Optionally, the waste liquid tank 91 is connected to the reaction assembly 4 for collecting the waste liquid of the reaction assembly 4. The reaction assembly 4 includes at least one reaction tank, and the waste liquid tank 91 is connected to an outlet of the reaction tank for collecting waste liquid in the reaction tank.

Optionally, a shutoff valve may be disposed between the waste liquid tank 91 and the liquid pump 92. When the liquid pump 92 has a sealing performance, the shut-off valve can also be changed to a one-way valve or the shut-off valve can be omitted.

Optionally, the sample analyzer 100 further includes a second waste liquid tank 94 for collecting waste liquid discharged under positive pressure driving, and a switching member 95, wherein the switching member 95 is connected. The switching member 95 is configured to communicate or shut off the second waste liquid tank 94 and the waste liquid tank 91 between the second waste liquid tank 94 and the waste liquid tank 91. The second waste liquid tank 94 has an interface that communicates with the atmosphere. When the switching member 95 communicates with the second waste liquid pool 94 and the waste liquid pool 91, the waste liquid in the second waste liquid pool 94 enters the waste liquid pool 91 under the pressure difference.

Referring to FIG. 1 to FIG. 3 together, as an alternative embodiment, the gas cylinder set 2 further includes a third gas storage tank 25. The first gas storage tank 21 is connected to the third gas storage tank 25 through a third control valve 26 for establishing a second positive pressure in the third gas storage tank 25 by a first positive pressure. The second positive pressure is less than or equal to the first positive pressure.

A third pressure sensor 73 is provided in the third air tank 25 for detecting the pressure in the third air tank 25. The controller 6 is coupled to the third control valve 26 for controlling the operation of the third control valve 26. When the third pressure sensor 73 detects that the pressure of the second positive pressure in the third gas storage tank 25 is insufficient, the controller 6 controls the third control valve 26 to communicate with the third storage. a gas tank 25 and the first gas storage tank 21, wherein the first positive pressure is built in the third gas storage tank 25 Pressing to raise the pressure of the second positive pressure. After the third pressure sensor 73 detects that the pressure of the second positive pressure reaches the demand, the controller 6 controls the third control valve 26 to cut off the third air tank 25 and the first storage Gas tank 21.

Optionally, the sample analyzer 100 further includes a sixth control valve 252 and a first restrictor 253. The sixth control valve 252 is connected between the third air tank 25 and the first restrictor 253. The first restrictor 253 is for releasing a part of the pressure in the third air tank 25.

The process of establishing the second positive pressure in the third gas storage tank 25 by the first positive pressure tank 21 is: the third control valve 26 is electrically connected to the first gas storage tank 21 The third gas storage tank 25 is configured to establish a pressure in the third gas storage tank 25 to form a second positive pressure whose absolute value is greater than a third preset value; The control valve 252 is connected to the third air tank 25 and the first restrictor 253, and the first restrictor 253 releases a part of the pressure in the third air tank 25 to make the pressure of the second positive pressure The absolute value is lowered to the third preset value. The pressure-building process eliminates both overshoot and rebound and achieves accurate pressure build-up.

Optionally, the first restrictor 253 is a restrictor, a restrictor or a restrictor.

Optionally, the third control valve 26 and/or the sixth control valve 252 are a shut-off valve or a two-position two-way valve.

Optionally, the sample analyzer 100 further includes a sheath liquid pool 51 and a flow chamber 52. The outlet of the sheath liquid pool 51 is connected to the sheath liquid inlet of the flow chamber 52. The third gas tank 25 communicates with the sheath liquid pool 51 for pushing the sheath liquid in the sheath liquid pool 51 into the flow chamber 52. The outlet of the flow chamber 52 is provided with an optical detection assembly 53 for detecting the number of cells by optical detection. The optical detection assembly 53 can be part of the detection assembly 5. The liquid to be tested entering the flow chamber 52 is pressure-driven to be detected by the optical detecting unit 53 to obtain a detection signal.

In this embodiment, since the second positive pressure in the third gas storage tank 25 can achieve accurate pressure build-up through the above-mentioned pressure-building process, the second positive pressure can satisfy the preset condition and stably The sheath liquid is pushed into the flow chamber 52 so that the optical detecting component 53 can obtain an accurate detection result by detecting the liquid to be tested.

Optionally, the controller 6 is coupled to the third control valve 26 for disconnecting the sheath fluid in the sheath liquid pool 51 through the third control valve 26 when flowing into the flow chamber 52 The third gas storage tank 25 and the first gas storage tank 21.

Optionally, the third pressure sensor 73 of the third gas storage tank 25 is further configured to disconnect the first gas storage tank 21 and the third gas storage tank 25 at the third control valve 26 When the sheath liquid in the sheath liquid pool 51 flows to the flow chamber 52, the pressure in the third air tank 25 and/or the sheath liquid pool 51 is detected.

In this embodiment, since the first gas storage tank 21 no longer supplements the second positive pressure in the third gas storage tank 25, the second positive pressure is when the sheath liquid is driven. The variation of the second positive pressure detected by the third pressure sensor 73 can accurately feed back the flow state of the sheath liquid, thereby providing reliable detection of whether the detection result of the optical detecting component 53 is accurate. The reference basis is such that the detection result provided by the sample analyzer 100 is reliable.

Referring to FIG. 1 , FIG. 2 and FIG. 4 together, as an alternative embodiment, the gas storage tank set 2 further includes a fourth gas storage tank 27, and the first gas storage tank 21 passes through the fourth control valve. The fourth gas storage tank 27 is connected to establish a third positive pressure in the fourth gas storage tank 27 by the first positive pressure. The third positive pressure is less than or equal to the first positive pressure. It will be understood by those skilled in the art that a third positive pressure can also be established in the fourth gas storage tank 27 through the third gas storage tank 25 if the stability requirements of the build pressure speed and the second positive pressure are not high.

A fourth pressure sensor 74 is disposed in the fourth gas storage tank 27 for detecting the pressure in the fourth gas storage tank 27. The controller 6 is connected to the fourth control valve 28 for controlling the action of the fourth control valve 28. When the fourth pressure sensor 74 detects that the pressure of the third positive pressure in the fourth gas storage tank 27 is insufficient, the controller 6 controls the fourth control valve 28 to communicate with the fourth storage. In the gas tank 27 and the first gas storage tank 21, the first positive pressure is built in the fourth gas storage tank 27 to increase the pressure of the third positive pressure. After the fourth pressure sensor 74 detects that the pressure of the third positive pressure reaches the demand, the controller 6 controls the fourth control valve 28 to cut off the fourth gas storage tank 27 and the first storage Gas tank 21. Similarly, a pressure building process similar to that of the third gas storage tank 25 can be used to build pressure on the fourth gas storage tank 27, which can eliminate both overshoot and rebound and achieve accurate construction. Pressure.

Optionally, the sample analyzer 100 includes a reservoir 41 and a first reaction tank 42 connected to the first reaction tank 42. The fourth gas storage tank 27 communicates with the liquid storage tank 41 to provide a driving force for the reagents in the liquid storage tank 41 to enter the first reaction tank 42. The reservoir 41 can be used to store reagents such as diluents, hemolytics or dyes. The first reaction pool 42 can be: used for Biological samples to form a reaction cell for the test solution for detecting hemoglobin counts, for processing biological samples to form a test solution for detecting white blood cell counts (and/or nucleated red blood cell classification and/or basophil classification) a cell, a reaction cell for processing a biological sample to form a test solution for detecting leukocyte classification, a reaction cell for processing a biological sample to form a test solution for detecting a red blood cell count, or for processing a biological sample to form a test reticulocyte Count the reaction cell of the test solution.

Referring to FIG. 1, FIG. 2 and FIG. 5 together, as an alternative embodiment, the sample analyzer 100 further includes a metering pump 43. The metering pump 43 has a diaphragm 431 and a liquid chamber 432 and a gas chamber 433 located on both sides of the diaphragm 431. The metering pump 43 is connected to the gas tank group 2, which is capable of providing the positive pressure to the metering pump 43. For example, the metering pump 43 is connected to the fourth gas tank 27 in the gas tank group 2, and the fourth gas tank 27 supplies the third pump with the third positive pressure. When the liquid chamber 432 communicates with the gas tank group 2, the positive pressure pushes the diaphragm 431 to move in the direction of the gas chamber 433, and when the gas chamber 433 communicates with the gas tank group 2, The positive pressure pushes the diaphragm 431 to move in the direction of the liquid chamber 432. When the diaphragm 431 moves in the direction of the gas chamber 433, the volume of the liquid chamber 432 increases, liquid enters the liquid chamber 432, and the metering pump 43 completes liquid absorption. When the diaphragm 431 moves in the direction of the liquid chamber 432, the volume of the liquid chamber 432 decreases, the liquid flows out of the liquid chamber 432, and the metering pump 43 completes the draining.

In this embodiment, since the liquid suction operation and the liquid discharge operation of the metering pump 43 are all completed by the positive pressure driving, that is, the metering pump 43 adopts the bidirectional positive pressure driving mode, the driving difficulty is small, which is favorable for reducing. The gas consumption of the gas cylinder group 2, thereby reducing the energy consumption of the sample analyzer 100. At the same time, since the metering pump 43 does not need to be driven by the negative pressure, the sample analyzer 100 can achieve accurate control of the positive pressure environment, thereby facilitating stable control of the operation of the metering pump 43 and avoiding instability due to the negative pressure environment. The liquid suction operation and the liquid discharge operation of the metering pump 43 are caused to be unstable.

In another embodiment, the metering pump 43 can also be connected to the first gas storage tank 21, which provides the first positive pressure to the metering pump 43. In still another embodiment, the metering pump 43 can also be coupled to the third gas storage tank 25, which provides the second positive pressure to the metering pump 43.

Optionally, the sample analyzer 100 includes a reservoir 41 and a first reaction cell 42. The liquid chamber 432 is connected between the reservoir 41 and the first reaction tank 42. The metering pump 43 is capable of quantitatively inputting the reagent in the reservoir 41 into the first reaction tank 42. The metering pump 43 also When the reagent in the reservoir 41 is insufficient, the first reaction tank 42 is provided with a backup reagent, so that the first reaction tank 42 can obtain a continuous liquid supply, thereby improving the sample analyzer 100. Detection speed. The sample analyzer 100 can also be provided with a control valve 45 that connects the reservoir 41, the first reaction chamber 42, and the liquid chamber 432 for achieving communication and shutoff. For example, the control valve 45 may communicate the liquid pool 41 and the liquid chamber 432 and cut off the first reaction tank 42 and the liquid chamber 432, and the liquid in the liquid storage tank 41 enters the liquid. Room 432, the metering pump 43 aspirate; or, the control valve 45 can communicate with the liquid chamber 432 and cut off the reservoir 41 and the liquid chamber 432, the liquid in the liquid chamber 432 enters In the first reaction tank 42, the metering pump 43 discharges liquid.

In one embodiment, the reservoir 41 is used to store reagents such as diluents, hemolytics or dyes. The first reaction cell 42 is a reaction cell for processing a biological sample to form a test solution for detecting a hemoglobin count, for processing a biological sample to form a detected white blood cell count (and/or nucleated red blood cell classification and/or basophilicity). a granulocyte classification) a reaction cell of a test solution, a reaction cell for processing a biological sample to form a test solution for detecting white blood cell classification, a reaction cell for processing a biological sample to form a test liquid for detecting a red blood cell count, or The biological sample is processed to form a reaction cell for the test solution for detecting the reticulocyte count.

In one embodiment, the reservoir 41 is for storing a diluent, the liquid chamber 432 stores a diluent, and the metering pump 43 is capable of being insufficient when the diluent in the reservoir 41 is insufficient. The first reaction cell 42 is temporarily supplied with a diluent to ensure that the diluent is continuously supplied to the first reaction cell 42 to increase the detection speed of the sample analyzer 100.

in particular:

When the control valve 45 communicates with the liquid reservoir 41 and the liquid chamber 432, the diluent in the reservoir 41 enters the liquid chamber 432 to form a reserve diluent. When the first reaction tank 42 requires a diluent, if the diluent in the reservoir 41 is sufficient, the control valve 45 communicates with the reservoir 41 and the first reaction tank 42, the reservoir The diluent in the liquid pool 41 enters the first reaction tank 42. If the diluent in the reservoir 41 is insufficient, the control valve 45 disconnects the reservoir 41 from the first reaction tank 42 and communicates the liquid chamber 432 with the first reaction tank 42, the diluent in the liquid chamber 432 enters the first reaction tank 42 to continuously supply a diluent to the first reaction tank 42. At this time, the liquid storage tank 41 can timely extract the diluent from the reagent tank for replenishment. E.g, The liquid storage tank 41 communicates with the second gas storage tank 22 to extract the diluent from the reagent tank by using the first negative pressure. When the dilution liquid in the liquid storage tank 41 is sufficient, the control valve 45 communicates again with the liquid storage tank 41 and the first reaction tank 42, and the liquid storage tank 41 continues to be the first reaction tank. 42 provides a diluent. In other embodiments, the spare diluent in the liquid chamber 432 can also be directly extracted from the reagent tank.

Referring to FIG. 1 , FIG. 2 , FIG. 6 and FIG. 7 , as an alternative embodiment, the gas storage tank set 2 further includes a fifth gas storage tank 29 , and the second gas storage tank 22 passes through A fifth control valve 210 is coupled to the fifth gas storage tank 29 for establishing a second negative pressure in the fifth gas storage tank 29 by the first negative pressure. The absolute value of the pressure of the second negative pressure is less than or equal to the absolute value of the pressure of the first negative pressure.

A fifth pressure sensor 75 is provided in the fifth gas storage tank 29 for detecting the pressure in the fifth gas storage tank 29. The controller 6 is connected to the fifth control valve 210 for controlling the action of the fifth control valve 210. When the fifth pressure sensor 75 detects that the pressure of the second negative pressure in the fifth gas storage tank 29 is insufficient, the controller 6 controls the fifth control valve 210 to communicate with the fifth storage. In the gas tank 29 and the second gas storage tank 22, the second negative pressure is built in the fifth gas storage tank 29 to lower the pressure of the second negative pressure. After the fifth pressure sensor 75 detects that the pressure of the second negative pressure reaches the demand, the controller 6 controls the fifth control valve 210 to cut off the fifth gas storage tank 29 and the second storage Gas tank 22.

Optionally, the sample analyzer 100 further includes a seventh control valve 292 and a second restrictor 293 connected to the fifth gas storage tank 29 and the second current limiting member Between 293. The second restrictor 293 is for releasing a part of the pressure in the fifth air tank 29.

The process of establishing the second negative pressure in the fifth gas storage tank 29 by the second gas storage tank 22 is: the fifth control valve 210 is electrically connected to the second gas storage tank 22 The fifth gas storage tank 29 is configured to compress the second negative pressure in the fifth gas storage tank 29 to form a second negative pressure whose absolute value is greater than a fourth preset value; The control valve 292 is connected to the fifth gas storage tank 29 and the second flow restricting member 293, and the second restrictor 293 releases a part of the pressure in the fifth gas storage tank 29 to make the pressure of the second negative pressure. The absolute value is raised to the fourth preset value. The pressure-building process eliminates both overshoot and rebound and achieves accurate pressure build-up.

Optionally, the second restrictor 293 is a restrictor tube, a restrictor valve or a restrictor.

Optionally, the fifth control valve 210 and/or the seventh control valve 292 are a shut-off valve or two positions. Two-way valve.

Optionally, the sample analyzer 100 further includes a second reaction tank 44 that is connected to an outlet of the second reaction tank 44. When the outlet of the second reaction tank 44 communicates with the fifth gas storage tank 29, the liquid in the second reaction tank 44 flows into the fifth gas storage tank 29 under the driving of the second negative pressure.

For example, an impedance detecting component 54 is provided at the outlet of the second reaction cell 44 for detecting the number of red blood cells by an impedance method (Coulter's principle). The second reaction cell 44 is a reaction cell for processing a biological sample to form a test solution for detecting a red blood cell count. The impedance method is quantified by using a time measurement method (ie, statistical volume = flow rate through the detection small hole × statistical time). Since the statistical time is fixed, the flow rate through the detection small hole determines the detection volume and directly affects the measurement result. . The impedance detecting component 54 is part of the detection component 5. The sample analyzer 100 drives the liquid to be tested in the second reaction cell 44 by the second negative pressure by establishing a stable and accurate second negative pressure in the fifth gas storage tank 29. The impedance detecting component 54 is thereby detected by the impedance detecting component 54 to obtain a detection signal, so that the flow rate of the liquid to be tested through the impedance detecting component 54 is stabilized, so that the impedance detecting component 54 is to be tested. The detection results of the liquid are more accurate and reliable.

As shown in FIG. 2, in an embodiment, the fifth control valve 210 is directly connected to the second gas storage tank 22. As shown in FIG. 7, in another embodiment, the fifth control valve 210 is connected to the second gas storage tank 22 via the waste liquid tank 91 (and the buffer tank 93). The second gas storage tank 22 communicates with the waste liquid pool 91, and the waste liquid pool 91 has the same or similar pressure as the second gas storage tank 22, that is, the pressure in the waste liquid tank 91 is The first negative pressure is close to the first negative pressure. At this time, the second negative pressure can be established in the fifth gas storage tank 29 by the pressure in the waste liquid tank 91. The waste liquid tank 91 is also used to collect the waste liquid in the fifth gas storage tank 29. The waste liquid in the waste liquid tank 91 can be discharged through the liquid pump 92.

Referring to FIG. 1 to FIG. 8 , an embodiment of the present invention further provides a driving method of the sample analyzer 100, which can be applied to the sample analyzer 100 described in the foregoing embodiment.

The driving method includes:

Driving the air pump 1 to establish a positive pressure and a negative pressure in the gas tank group 2;

The positive pressure and the negative pressure drive the flow path of the sample analyzer 100.

In the embodiment, the driving method establishes positive in the gas tank group 2 by the air pump 1 The pressure and the negative pressure are then used as the main driving force of the sample analyzer 100 through the positive pressure and the negative pressure in the gas tank group 2, thereby being able to replace the large-flow air pump 1 in the prior art, thereby reducing the The cost and energy consumption of the sample analyzer 100. Meanwhile, since the driving method can employ the small volume of the air pump 1, the overall volume of the sample analyzer 100 can be reduced.

As an alternative embodiment, the “driving the air pump 1 to establish a positive pressure and a negative pressure in the gas storage tank set 2” includes: driving the air pump 1 to establish a first positive pressure in the first gas storage tank 21, A first negative pressure is established within the second gas reservoir 22.

The action of the air pump 1 to establish the first positive pressure and the act of establishing the first negative pressure may be performed in a staggered manner or may be performed simultaneously. The first positive pressure is used to provide a primary positive pressure driving force to the sample analyzer 100. The first negative pressure is used to provide a primary negative pressure driving force to the sample analyzer 100.

Optionally, the process of driving the air pump 1 to establish a first positive pressure in the first gas storage tank 21 includes:

The air pump 1 establishes a first positive pressure in the first gas storage tank 21 that the absolute value of the pressure is greater than a first preset value; and

The first gas storage tank 21 is turned on to the atmosphere, and the absolute value of the first positive pressure is lowered to the first predetermined value.

In this embodiment, the process of establishing the first positive pressure can eliminate the two phenomena of overshoot and rebound, and achieve accurate pressure build-up.

Optionally, the process of driving the air pump 1 to establish a first negative pressure in the second gas storage tank 22 includes:

The air pump 1 establishes in the second gas storage tank 22 a first negative pressure whose absolute value is greater than a second preset value;

The second gas storage tank 22 is turned on to the atmosphere, and the absolute value of the pressure of the second negative pressure is lowered to the second preset value.

In this embodiment, the process of establishing the first negative pressure can eliminate the two phenomena of overshoot and rebound, and achieve accurate pressure build-up.

As an alternative embodiment, when the air pump 1 of the sample analyzer 100 is pressure-built with a low-cost one-way pressurized single-head pump, the air pump 1 needs to be separately the first storage. Gas tank 21 And the second gas storage tank 22 is pressurized, and the pressure building principles of the first gas storage tank 21 and the second gas storage tank 22 are as follows:

The pressure of the first positive pressure P1 in the first gas storage tank 21 is divided into three pressure levels: a first threshold A1, a third threshold A2, and a fifth threshold A3, and the first threshold A1 is smaller than the third The threshold A2 (A1 < A2), the third threshold A2 is smaller than the fifth threshold A3 (A2 < A3). The pressure of the first negative pressure P2 in the second gas storage tank 22 is divided into two pressure levels: a second threshold B1 and a fourth threshold B2, and the second threshold B1 is smaller than the fourth threshold B2 (B1< B2).

When the absolute value of the pressure of the first positive pressure P1 is less than the first threshold A1 (|P1|<A1), the priority is the highest, and a positive pressure is established at this time, when the pressure reaches the first threshold A1 is established. (|P1|≥A1), the pressure build process ends.

When the actual pressure absolute value of the first negative pressure P2 is less than the second threshold B1 (|P2|<B1), the priority is the second highest, and the negative pressure is established at this time, when the pressure reaches the second At the threshold B1 (|P2| ≥ B1), the build-up process ends.

When the absolute value of the pressure of the first positive pressure P1 is greater than or equal to the first threshold A1 and less than the third threshold A2 (A1≤|P1|<A2), the priority is the third highest, and a positive pressure is established at this time. When the pressure reaches the third threshold A2 (P1|≥ A2), the pressure building process ends.

When the absolute value of the pressure of the first negative pressure P2 is greater than or equal to the second threshold B1 and less than the fourth threshold B2 (B1≤|P2|<B2), the priority is the fourth highest, and the negative pressure is established at this time. When the pressure reaches the fourth threshold B2 (|P2| ≥ B2), the pressure building process ends.

When the absolute value of the pressure of the first positive pressure P1 is greater than or equal to the third threshold A2 and less than the fifth threshold A3 (A2≤|P1|<A3), the priority is the fifth highest, and a positive pressure is established at this time. When the pressure reaches the fifth threshold A3 (P1|≥A3), the pressure building process ends.

In other words:

When the absolute value of the pressure of the first positive pressure P1 is less than the first threshold A1 (|P1|<A1), the air pump 1 is driven to build a pressure in the first gas storage tank 21 to make the first positive pressure The absolute value of the pressure of P1 reaches the first threshold A1 (|P1| ≥ A1).

When the absolute value of the pressure of the first positive pressure P1 is greater than or equal to the first threshold A1 (|P1|≥ A1) and the absolute value of the pressure of the first negative pressure P2 is less than the second threshold B1 (|P2|<B1), Driving the air pump 1 to establish a pressure in the second gas storage tank 22, so that the absolute value of the pressure of the first negative pressure P2 reaches the second Threshold B1 (|P2| ≥ B1).

The absolute value of the pressure of the first positive pressure P1 is greater than or equal to the first threshold A1 and less than the third threshold A2 (A1≤|P1|<A2), and the absolute value of the pressure of the first negative pressure P2 is greater than or equal to the second threshold B1 (B1≤|P2|), driving the air pump 1 to establish a pressure in the first gas storage tank 21, so that the absolute value of the pressure of the first positive pressure P1 reaches the third threshold A2 (P1|≥ A2).

The absolute value of the pressure of the first positive pressure P1 is greater than or equal to the third threshold A2 (A2≤|P1|), and the absolute value of the pressure of the first negative pressure P2 is greater than or equal to the second threshold B1 and less than the fourth threshold B2 (B1) ≤|P2|<B2), driving the air pump 1 to establish a pressure in the second gas storage tank 22, so that the absolute value of the pressure of the first negative pressure P2 reaches the fourth threshold value B2 (|P2| ≥B2).

The absolute value of the pressure of the first positive pressure P1 is greater than or equal to the third threshold A2 and less than the fifth threshold A3 (A2≤|P1|<A3), and the absolute value of the pressure of the first negative pressure P2 is greater than or equal to the fourth threshold B2. (|P2| ≥ B2), driving the air pump 1 to establish a pressure in the first gas storage tank 21 such that the absolute value of the pressure of the first positive pressure P1 reaches the fifth threshold A3 (P1| ≥ A3).

In this embodiment, the driving method is based on the urgency of the first positive pressure and the first negative pressure demand of the sample analyzer 100, and the air pump 1 is set reasonably and skillfully to establish the first The timing and extent of the positive pressure and the first negative pressure enable the first positive pressure and the first negative pressure to better achieve the driving action. It can be understood that the pressure forming process of the first gas storage tank 21 and the second gas storage tank 22 can be monitored at any time to ensure the smooth operation of the sample analyzer 100.

Optionally, the sample analyzer 100 further includes a waste liquid pool 91 and a liquid pump 92. The waste liquid pool 91 is connected to the second gas storage tank 22, and the liquid pump 92 is used to extract the waste liquid pool. The waste liquid in 91 establishes a negative pressure in the waste liquid tank 91.

The pressure-building operation of the liquid pump 92 and the pressure-building operation of the air pump 1 are independent of each other. The rules for the liquid pump 92 to discharge waste liquid and establish a negative pressure are as follows:

The pressure of the first negative pressure P2 in the second gas storage tank 22 further includes two pressure levels: a sixth threshold B3 and a seventh threshold B4, and the fourth threshold B2 is smaller than the sixth threshold B3 (B2) <B3), the sixth threshold B3 is smaller than the seventh threshold B4 (B3 < B4).

When the absolute value of the pressure of the first negative pressure P2 is less than the sixth threshold B3 (|P2|<B3), the liquid pump 92 is turned on to discharge the liquid pump 92 into the waste liquid pool 91. Waste liquid and establishing a negative pressure in the waste liquid tank 91; and

When the absolute value of the first negative pressure is greater than or equal to the seventh threshold B4 (|P2| ≥ B4), the liquid pump 92 is turned off.

In this embodiment, since the fourth threshold is smaller than the sixth threshold, the liquid pump 92 is in a state of discharging waste liquid and assisting pressure build-up for a majority of time, and the liquid pump 92 can discharge the The waste liquid in the waste liquid tank 91, the inside of the waste liquid tank 91 is almost empty, so that waste liquid or bubbles or the like can be prevented from being poured into the second gas storage tank 22 and the air pump 1, so that the sample The analyzer 100 is capable of working normally for a long time. Since the liquid pump 92 can assist in establishing the negative pressure, it is possible to solve the problem that the negative pressure flow which the gas pump 1 of a small flow rate may have is insufficient. Since the waste liquid in the waste liquid tank 91 is discharged to the outside of the machine through the liquid pump 92 for discharge, it is not necessary to switch the pressure in the waste liquid tank 91, and the waste liquid pool 91 can always maintain a negative pressure state. So that the waste liquid pool 91 can continuously extract the waste liquid in the sample analyzer 100 through its internal negative pressure, so that the waste liquid action and the discharge waste liquid action of the waste liquid processing assembly can be performed in parallel and mutually The waste liquid treatment efficiency of the waste liquid processing assembly is high without interference, and the whole machine measurement speed of the sample analyzer 100 is fast. The negative pressure is always maintained in the waste liquid tank 91, and there is no need to perform positive and negative pressure switching, so that the increase of the air consumption caused by the switching of the positive and negative pressures can be avoided, and the air pump 1 which is advantageous for the small flow rate can better satisfy the said The drive requirements of the sample analyzer 100.

As an alternative embodiment, the first positive pressure establishes a second positive pressure within the third gas reservoir 25. The second positive pressure is less than or equal to the first positive pressure.

Optionally, the process of “the first positive pressure establishes a second positive pressure in the third gas storage tank 25” includes:

The first gas storage tank 21 and the third gas storage tank 25 are turned on, so that the first positive pressure is built in the third gas storage tank 25 to form an absolute value of the pressure greater than the third preset. The second positive pressure of the value; and

The third gas storage tank 25 is turned to the atmosphere, and the absolute value of the pressure of the second positive pressure is lowered to the third preset value.

In this embodiment, the process of establishing the second positive pressure can eliminate the two phenomena of overshoot and rebound, and achieve accurate pressure build-up. As shown in FIG. 8, the line segment 01, the line segment 02, and the line segment 03 in FIG. 8 represent the change process of the second positive pressure. As can be seen from FIG. 8, the above-mentioned pressure forming method may accurately establish the second positive pressure in the The third preset value P is described.

Optionally, when the second positive pressure is lower than the third preset value, turning on the first gas storage tank 21 and the third gas storage tank 25 to achieve the second positive pressure The third preset value.

As an alternative embodiment, the second positive pressure pushes the sheath fluid within the sheath fluid pool 51 into the flow chamber 52. The driving method pushes the sheath liquid with the precise second positive pressure, which facilitates obtaining an accurate detection result by the optical detecting component 53 provided in the flow chamber 52. The sheath liquid pool 51 can be kept in communication with the third gas storage tank 25 such that the pressure in the sheath liquid pool 51 is the same as that of the third gas storage tank.

Optionally, the third gas storage tank 25 and the first gas storage tank 21 are disconnected before the second positive pressure pushes the sheath liquid in the sheath liquid pool 51 into the flow chamber 52.

In this embodiment, since the third gas storage tank 25 and the first gas storage tank 21 are disconnected, the first gas storage tank 21 is no longer in the third gas storage tank 25 The second positive pressure is supplemented, so that the fluctuation range of the second positive pressure is small, and the second positive pressure can stably drive the sheath liquid, which is advantageous for the optical detecting component 53 to obtain an accurate detection result. . It can be understood that when the third gas storage tank 25 communicates with the first gas storage tank 21 for pressure build-up, the sheath liquid pool 51 and the flow chamber 52 can be disconnected through a shut-off valve. After the third air tank 25 is disconnected from the first air tank 21, the shutoff valve is opened, so that the second positive pressure pushes the sheath liquid in the sheath liquid pool 51 into the flow chamber 52.

Alternatively, when the second positive pressure pushes the sheath liquid in the sheath liquid pool 51 into the flow chamber 52, the pressure change of the second positive pressure is detected by the third pressure sensor 73. Since the first gas storage tank 21 no longer supplements the second positive pressure in the third gas storage tank 25, the second positive pressure is continuously decreased when the sheath liquid is driven, so The change of the second positive pressure detected by the third pressure sensor 73 can accurately feed back the flow state of the sheath liquid, thereby providing a reliable reference for whether the detection result of the optical detecting component 53 is accurate, so that The test results provided by the sample analyzer 100 are reliable.

Optionally, when the pressure change of the second positive pressure does not satisfy the preset condition, an alarm is issued. If the pressure change of the second positive pressure does not satisfy the preset condition, the second positive pressure pushes the outflow of the sheath liquid in the sheath liquid pool 51 to be unstable, which directly affects the sample analysis. The accuracy of the test result of the meter 100. At this time, the driving method performs an alarm, and can remind the user that the corresponding detection result is inaccurate. If the pressure change of the second positive pressure satisfies a preset condition, the detection result of the sample analyzer 100 is accurate. Therefore, the sample analyzer 100 to which the driving method is applied can provide a reliable detection result.

Optionally, the driving method may perform corresponding maintenance on the sample analyzer 100 after an alarm (Cleaning or re-establishing pressure, etc.) to ensure the accuracy of the test results for the next test.

In one embodiment, the pressure curve is formed according to the pressure change, and when the slope of the pressure curve is not within the preset range, it is determined that the pressure change does not satisfy the preset condition. When the slope of the pressure curve is within a preset range, it is judged that the pressure change satisfies a preset condition, and the second positive pressure pushes the action of the sheath liquid in the sheath liquid pool 51 to be stable. As shown in FIG. 8, the line segment 04 represents the pressure change of the second positive pressure. When the slope of the line segment 04 is within a preset range, it is judged that the pressure change satisfies a preset condition, when the slope of the line segment 04 is not When the preset range is within, it is judged that the pressure change does not satisfy the preset condition.

In another embodiment, the pressure value data set is formed according to the pressure change, and when the difference of the data in the pressure value data group is not within the preset range, it is determined that the pressure change does not satisfy the preset condition. When the difference of the data in the pressure value data group is within a preset range, determining that the pressure change satisfies a preset condition, the second positive pressure pushing the sheath liquid in the sheath liquid pool 51 to flow out The action is stable.

As an alternative embodiment, the first positive pressure establishes a third positive pressure within the fourth gas reservoir 27. The third positive pressure is less than or equal to the first positive pressure.

Optionally, the liquid storage tank 41 is connected to the first reaction tank 42, and the fourth gas storage tank 27 is connected to the liquid storage tank 41 to use the third positive pressure to the liquid storage tank 41. The reagent inside is pushed into the first reaction cell 42.

Optionally, when the third positive pressure is lower than the fifth preset value, the first gas storage tank 21 and the fourth gas storage tank 27 are turned on, so that the third positive pressure reaches the The fifth preset value.

As an alternative embodiment, the first negative pressure establishes a second negative pressure within the fifth gas reservoir 29. The absolute value of the pressure of the second negative pressure is less than or equal to the absolute value of the pressure of the first negative pressure.

Optionally, the process of “the first negative pressure establishes a second negative pressure in the fifth gas storage tank 29” includes:

The fifth gas storage tank 29 and the second gas storage tank 22 are turned on, so that the first negative pressure is built in the fifth gas storage tank 29 to form an absolute value of the pressure greater than the fourth preset. a second negative pressure of the value; and

The fifth gas storage tank 29 is turned on to the atmosphere, and the absolute value of the pressure of the second negative pressure is lowered to the fourth preset value.

In this embodiment, the process of establishing the second negative pressure can eliminate the two phenomena of overshoot and rebound, and achieve accurate pressure build-up.

Optionally, when the second negative pressure is lower than the fourth preset value, turning on the second air tank 22 and The fifth gas storage tank 29 is configured to bring the second negative pressure to the fourth preset value.

Optionally, the fifth gas storage tank 29 is turned to the outlet of the second reaction tank 44 to extract the liquid in the second reaction tank 44 by the second negative pressure. When the outlet of the second reaction tank 44 communicates with the fifth gas storage tank 29, the liquid in the second reaction tank 44 flows into the fifth gas storage tank 29 under the driving of the second negative pressure.

In the present embodiment, an impedance detecting component 54 may be provided at the exit of the second reaction cell 44 for detecting the number of red blood cells by the impedance method (Coulter's principle). The liquid to be tested passes through the impedance detection component when the liquid to be tested in the second reaction cell 44 passes through the impedance detecting component 54 by the second negative pressure. The flow rate of the component 54 is stable, so the detection result of the impedance detecting component 54 to the liquid to be tested is more accurate and reliable.

As an alternative embodiment, the metering pump 43 of the sample analyzer 100 includes a liquid chamber 432 and a plenum 433. The liquid chamber 432 is connected to the reservoir 41 and the reaction cell. The driving method further includes:

The reservoir 41 communicates with the positive pressure to push the liquid in the reservoir 41 into the liquid chamber 432 by the positive pressure;

The gas chamber 433 communicates with the positive pressure to push the liquid in the liquid chamber 432 toward the reaction cell by the positive pressure.

In the present embodiment, the liquid absorption operation of the metering pump 43 (the liquid in the reservoir 41 enters the liquid chamber 432) and the liquid discharge operation (the liquid in the liquid chamber 432 flows to the reaction) The pool is driven by the positive pressure (for example, the first positive pressure, the second positive pressure or the third positive pressure), that is, the quantitative pump 43 adopts a bidirectional positive pressure driving mode, and the driving difficulty is Small, it is advantageous to reduce the air consumption of the gas tank group 2, thereby reducing the energy consumption of the sample analyzer 100. At the same time, since the metering pump 43 does not need to be driven by the negative pressure, the sample analyzer 100 can achieve accurate control of the positive pressure environment, thereby facilitating stable control of the operation of the metering pump 43 and avoiding instability due to the negative pressure environment. The liquid suction operation and the liquid discharge operation of the metering pump 43 are caused to be unstable.

The embodiments of the present invention have been described in detail above, and the principles and implementations of the present invention are described in detail herein. The description of the above embodiments is only for helping to understand the method of the present invention and its core ideas; It should be understood by those skilled in the art that the present invention is not limited by the scope of the present invention.

Claims (41)

1. A sample analyzer, comprising: an air pump, a gas storage tank set, a sampling assembly, a reaction assembly, and a detection assembly, wherein the air pump is configured to establish a positive pressure and a negative pressure in the gas storage tank group, the positive The pressure and the negative pressure are used to:

   Driving the sampling component to collect a biological sample;

   And/or driving the reaction assembly to process the biological sample to form a test solution, the reaction assembly comprising at least one reaction cell;

   And/or driving the test solution to be detected by the detecting component to obtain a detection signal.
2. The sample analyzer according to claim 1, wherein said gas tank set comprises a first gas storage tank and a second gas storage tank, said gas pump being connected to said first gas storage tank through a first control valve a first positive pressure is established in the first gas storage tank, and the air pump is connected to the second gas storage tank through a second control valve for establishing a first negative in the second gas storage tank Pressure.
3. A sample analyzer according to claim 2, wherein said air pump is a single-head pump for said first gas storage when said first control valve is turned on and said second control valve is turned off The tank builds pressure and builds pressure on the second gas tank when the first control valve is shut off and the second control valve is turned on.
4. A sample analyzer according to claim 2, wherein said air pump is a single-head pump or a double-head pump for use when said first control valve is turned on and said second control valve is turned on The first gas storage tank and the second gas storage tank are built.
5. The sample analyzer according to claim 2, wherein said sample analyzer further comprises a controller and a pressure sensor group for detecting a pressure in said gas tank group and feeding back a signal to The controller, the controller controls an action of the air pump, the first control valve, and the second control valve according to the signal.
6. The sample analyzer according to any one of claims 2 to 5, wherein at least one pressure cut valve is provided on a flow path of the sample analyzer, and the first positive pressure is used to drive the pressure cut valve. .
7. The sample analyzer according to claim 2, wherein said sample analyzer further comprises a waste liquid pool connected to said second gas storage tank and said liquid pump for extracting The waste liquid in the waste liquid pool.
8. A sample analyzer according to claim 7, wherein said waste liquid pool is provided with a first A float switch for detecting the liquid level in the waste liquid pool.
9. The sample analyzer according to claim 7, wherein said sample analyzer further comprises a buffer pool connected between said second gas storage tank and said waste liquid pool, said buffer pool And a method for preventing waste liquid in the waste liquid pool from being poured into the second gas storage tank.
10. The sample analyzer according to claim 7, wherein a second float switch is disposed in the second gas tank for detecting a liquid level in the second gas tank.
11. A sample analyzer according to claim 2, wherein said sample analyzer further comprises a waste liquid pool and a liquid pump, said liquid pump for extracting waste liquid in said waste liquid pool and said waste liquid A negative pressure is established in the pool.
12. The sample analyzer according to any one of claims 7 to 11, wherein the waste liquid pool is connected to the reaction assembly, and the waste liquid pool is used to collect the waste liquid of the reaction assembly.
13. The sample analyzer according to claim 5, wherein the gas tank set further comprises a third gas storage tank, wherein the first gas storage tank is connected to the third gas storage tank through a third control valve, And a second positive pressure is established in the third gas storage tank by the first positive pressure.
14. A sample analyzer according to claim 13, wherein said sample analyzer further comprises a sixth control valve and a first restrictor, said sixth control valve being coupled to said third gas tank and said Between the first restrictors, the first restrictor is used to release a part of the pressure in the third air tank.
15. A sample analyzer according to claim 13 or claim 14, wherein said sample analyzer further comprises a sheath liquid pool and a flow chamber, said outlet of said sheath liquid pool being connected to a sheath liquid inlet of said flow chamber, said A third gas storage tank is connected to the sheath liquid pool for pushing the sheath liquid in the sheath liquid pool into the flow chamber.
16. A sample analyzer according to claim 15 wherein said controller couples said third control valve for said third control when said sheath fluid in said sheath reservoir flows into said flow chamber The valve disconnects the third gas storage tank from the first gas storage tank.
17. A sample analyzer according to claim 15, wherein said pressure sensor group further comprises a third pressure sensor, said third pressure sensor for disconnecting said first one at said third control valve The pressure in the third gas tank and/or the sheath liquid pool is detected when the gas tank and the third gas tank and the sheath liquid in the sheath liquid pool flows toward the flow chamber.
18. A sample analyzer according to claim 5, wherein said gas tank group is further packaged The fourth gas storage tank is connected to the fourth gas storage tank through a fourth control valve for establishing a third positive pressure in the fourth gas storage tank by the first positive pressure.
19. A sample analyzer according to claim 18, wherein said sample analyzer comprises a reservoir and a first reaction cell, said reservoir being connected to said first reaction cell, said fourth gas storage tank The reservoir is connected to push reagents in the reservoir into the first reaction cell.
20. A sample analyzer according to claim 1 or 18, wherein said sample analyzer further comprises a metering pump having a diaphragm and a liquid chamber and a gas chamber on both sides of said diaphragm, said metering pump Connecting the gas storage tank group, the positive pressure pushes the diaphragm to move toward the gas chamber when the liquid chamber communicates with the gas storage tank group, and the gas chamber communicates with the gas storage tank group The positive pressure urges the diaphragm to move in the direction of the liquid chamber.
21. A sample analyzer according to claim 20, wherein said sample analyzer comprises a reservoir and a first reaction chamber, said chamber being connected between said reservoir and said first reaction chamber.
22. The sample analyzer according to claim 5, wherein the gas tank set further comprises a fifth gas storage tank, and the second gas storage tank is connected to the fifth gas storage tank through a fifth control valve. And a second negative pressure is established in the fifth gas storage tank by the first negative pressure.
23. A sample analyzer according to claim 22, wherein said sample analyzer further comprises a seventh control valve and a second restrictor, said seventh control valve being coupled to said fifth gas storage tank Between the second flow restricting members, the second restrictor is for releasing a part of the pressure in the fifth gas storage tank.
24. The sample analyzer according to claim 22 or 23, wherein the sample analyzer further comprises a second reaction tank, the fifth gas tank being connected to an outlet of the second reaction tank.
25. A driving method of a sample analyzer, characterized in that the driving method comprises:

    Driving the air pump to establish positive and negative pressures in the gas storage tank group; and

    The positive pressure and the negative pressure drive the flow path of the sample analyzer.
26. The driving method according to claim 25, wherein said "driving the air pump to establish positive pressure and negative pressure in the gas tank group" comprises:

    The air pump is driven to establish a first positive pressure in the first gas storage tank and a first negative pressure in the second gas storage tank.
27. The driving method according to claim 26, wherein when the absolute value of the first positive pressure is less than the first threshold, the air pump is driven to build a pressure in the first gas storage tank, so that the first One The absolute value of the positive pressure reaches the first threshold.
28. The driving method according to claim 26, wherein the air pump is driven when the absolute value of the first positive pressure is greater than or equal to the first threshold and the absolute value of the first negative pressure is less than the second threshold A pressure is built in the second gas storage tank such that the absolute value of the pressure of the first negative pressure reaches the second threshold.
29. The driving method according to claim 26, wherein the absolute value of the pressure of the first positive pressure is greater than or equal to a first threshold and less than a third threshold, and the absolute value of the pressure of the first negative pressure is greater than or equal to a second threshold And driving the air pump to establish a pressure in the first gas storage tank, so that the absolute value of the pressure of the first positive pressure reaches the third threshold.
30. The driving method according to claim 26, wherein the absolute value of the pressure of the first positive pressure is greater than or equal to a third threshold, and the absolute value of the pressure of the first negative pressure is greater than or equal to a second threshold and less than a fourth threshold And driving the air pump to establish a pressure in the second gas storage tank, so that the absolute value of the pressure of the first negative pressure reaches the fourth threshold.
31. The driving method according to claim 26, wherein the absolute value of the pressure of the first positive pressure is greater than or equal to a third threshold and less than a fifth threshold, and the absolute value of the pressure of the first negative pressure is greater than or equal to a fourth threshold And driving the air pump to establish a pressure in the first gas storage tank, so that the absolute value of the pressure of the first positive pressure reaches the fifth threshold.
32. The driving method according to any one of claims 26 to 31, wherein the first positive pressure establishes a second positive pressure in the third gas tank.
33. The driving method according to claim 32, wherein said second positive pressure pushes the sheath liquid in said sheath liquid pool into the flow chamber.
34. The driving method according to claim 33, wherein said third gas storage tank is disconnected from said first before said second positive pressure pushes said sheath liquid in said sheath liquid pool into said flow chamber gas tank.
35. The driving method according to claim 34, wherein "the second positive pressure pushes the sheath liquid in the sheath liquid pool into the flow chamber", the pressure of the second positive pressure is detected by the third pressure sensor Variety.
36. The driving method according to claim 32, wherein said first positive pressure establishes a third positive pressure in said fourth gas storage tank.
37. The driving method according to claim 36, wherein said liquid storage tank is connected to said first reaction tank, and said fourth gas storage tank is connected to said liquid storage tank to enter a reagent in said liquid storage tank The first reaction cell provides a driving force.
38. The driving method according to claim 36, wherein said first negative pressure establishes a second negative pressure in said fifth gas storage tank.
39. The driving method according to claim 38, wherein the fifth gas storage tank is opened to an outlet of the second reaction cell to extract the liquid in the second reaction cell by the second negative pressure.
40. The driving method according to claim 38, wherein:

    The process of driving the air pump to establish a first positive pressure in the first gas storage tank includes:

    The air pump establishes a first positive pressure in the first gas storage tank whose absolute value is greater than a first preset value, and turns on the first gas storage tank to the atmosphere, so that the pressure of the first positive pressure is absolutely Decreasing the value to the first preset value;

    and / or,

    The process of driving the air pump to establish a first negative pressure in the second gas storage tank includes:

    The air pump establishes a first negative pressure in the second gas storage tank whose absolute value is greater than a second preset value, and turns on the second gas storage tank to the atmosphere, so that the pressure of the second negative pressure is absolutely The value is lowered to the second preset value;

    and / or,

    The process of establishing the second positive pressure in the third gas storage tank by the first positive pressure comprises:

    Turning on the first gas storage tank and the third gas storage tank, so that the first positive pressure is built in the third gas storage tank to form a pressure absolute value greater than a third preset value a second positive pressure; conducting the third gas storage tank to the atmosphere, reducing the absolute value of the second positive pressure to the third predetermined value;

    and / or,

    The process of establishing the second negative pressure in the fifth gas storage tank by the first negative pressure comprises:

    Turning on the fifth gas storage tank and the second gas storage tank, so that the first negative pressure is built in the fifth gas storage tank to form a pressure absolute value greater than a fourth preset value Two negative pressures; conducting the fifth gas storage tank to the atmosphere, and decreasing the absolute value of the second negative pressure to the fourth predetermined value.
41. The driving method according to any one of claims 25 to 31, wherein the metering pump of the sample analyzer comprises a liquid chamber and a gas chamber, the liquid chamber is connected to the liquid storage tank and the first reaction tank, and the driving The method also includes:

    The liquid storage tank communicates with the positive pressure to push liquid in the liquid storage tank into the liquid chamber by using the positive pressure; and

    The plenum communicates the positive pressure to push liquid in the liquid chamber toward the first reaction chamber using the positive pressure.

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