CN110741257A - Sample analyzer and driving method thereof - Google Patents

Abstract

sample analyzers (100) and a driving method thereof, the sample analyzers comprise an air pump (1), an air storage tank group (2), a sampling assembly (3), a reaction assembly (4) and a detection assembly (5), wherein the air pump (1) is used for establishing positive pressure and negative pressure in the air storage tank group (2), the positive pressure and the negative pressure are used for driving the sampling assembly (3) to collect a biological sample, and/or driving the reaction assembly (4) to process the biological sample to form a liquid to be detected, the reaction assembly (4) comprises at least reaction cells, and/or driving the liquid to be detected by the detection assembly (5) to obtain a detection signal, and the sample analyzers (100) are low in cost.

Description

Sample analyzer and driving method thereof Technical Field

The invention relates to the technical field of medical instruments, in particular to sample analyzers and a driving method of sample analyzers.

Background

The existing sample analyzers require a variety of different drive pressures for providing different drive pressures for various lines in the sample analyzer. The driving pressure typically includes at least two positive pressures and at least two negative pressures. The sample analyzer can establish positive pressure and negative pressure simultaneously by adopting a large-flow and large-volume double-head air pump. The positive pressure output is used for establishing the highest positive pressure through a pressure relief valve, and other relatively low positive pressure is regulated and output by adopting different pressure regulating valves; the negative pressure output is established with the highest vacuum degree through a flow limiting pipe, and other negative pressures with lower vacuum degrees are output by adopting an overflow valve for regulation. However, the driving pressure in the sample analyzer of the above scheme always needs to be driven by a double-head air pump, and the cost is high.

Disclosure of Invention

The present invention is to provide types of sample analyzers with low cost and a method for driving types of sample analyzers.

In order to achieve the above purpose, the embodiment of the invention adopts the following technical scheme:

, there are sample analyzers that include an air pump for establishing positive and negative pressures within a set of air reservoirs, a sampling assembly, a reaction assembly, and a detection assembly, the positive and negative pressures being used to:

driving the sampling assembly to collect a biological sample;

and/or driving the reaction assembly to process the biological sample to form a liquid to be detected, wherein the reaction assembly comprises at least reaction cells;

and/or driving the liquid to be detected by the detection assembly to obtain a detection signal.

Wherein, the gas tank group includes th gas tank and second gas tank, the air pump passes through control valve and connects the th gas tank is used for establishing th malleation in the th gas tank, the air pump passes through the second control valve and connects the second gas tank is used for establishing th negative pressure in the second gas tank.

The air pump is a single-head pump and is used for pressurizing the air storage tank when the th control valve is switched on and the second control valve is switched off, and pressurizing the second air storage tank when the th control valve is switched off and the second control valve is switched on.

The air pump is a single-head pump or a double-head pump and is used for building pressure for the air storage tank and the second air storage tank when the -th control valve is switched on and the second control valve is switched on.

The sample analyzer further comprises a controller and a pressure sensor group, the pressure sensor group is used for detecting the pressure in the gas storage tank group and feeding back a signal to the controller, and the controller controls the actions of the air pump, the th control valve and the second control valve according to the signal.

Wherein, be equipped with at least pressure break valves on the flow path of sample analysis appearance, the positive pressure is used for driving the pressure break valve.

The sample analyzer further comprises a waste liquid pool and a liquid pump, wherein the waste liquid pool is connected with the second gas storage tank, and the liquid pump is used for pumping waste liquid in the waste liquid pool.

And an th float switch is arranged in the waste liquid pool and used for detecting the liquid level in the waste liquid pool.

The sample analyzer further comprises a buffer pool, the buffer pool is connected between the second gas storage tank and the waste liquid pool, and the buffer pool is used for preventing waste liquid in the waste liquid pool from flowing backwards into the second gas storage tank.

And a second float switch is arranged in the second air storage tank and used for detecting the liquid level in the second air storage tank.

The sample analyzer further comprises a waste liquid pool and a liquid pump, wherein the liquid pump is used for pumping waste liquid in the waste liquid pool and establishing negative pressure in the waste liquid pool.

The waste liquid pool is connected with the reaction assembly and is used for collecting waste liquid of the reaction assembly.

The air storage tank group further comprises a third air storage tank, and the th air storage tank is connected with the third air storage tank through a third control valve and used for establishing a second positive pressure in the third air storage tank through a th positive pressure.

Wherein the sample analyzer further comprises a sixth control valve connected between the third reservoir and the flow restrictor and an flow restrictor, the flow restrictor being configured to relieve a portion of pressure within the third reservoir.

The sample analyzer further comprises a sheath liquid pool and a flow chamber, wherein an outlet of the sheath liquid pool is connected with a sheath liquid inlet of the flow chamber, and the third air storage tank is communicated with the sheath liquid pool and used for pushing sheath liquid in the sheath liquid pool to flow into the flow chamber.

Wherein the controller is coupled to the third control valve for disconnecting the third air reservoir from the th air reservoir via the third control valve when sheath fluid within the sheath fluid reservoir flows into the flow chamber.

Wherein the pressure sensor group further comprises a third pressure sensor connected to detect a pressure in the third gas tank and/or the sheath fluid when the third control valve disconnects the th gas tank from the third gas tank and the sheath fluid in the sheath fluid flows to the flow chamber.

The th air tank is connected with the fourth air tank through a fourth control valve and is used for establishing a third positive pressure in the fourth air tank through a th positive pressure.

Wherein, the sample analysis appearance includes liquid reserve tank and th reaction tank, the liquid reserve tank is connected the th reaction tank, the fourth gas holder intercommunication the liquid reserve tank is used for with reagent in the liquid reserve tank impels the th reaction tank.

The sample analyzer further comprises a quantitative pump, the quantitative pump is provided with a diaphragm, a liquid chamber and an air chamber, the liquid chamber and the air chamber are located on two sides of the diaphragm, the quantitative pump is connected with the air tank group, when the liquid chamber is communicated with the air tank group, the diaphragm is pushed by positive pressure to move towards the direction of the air chamber, and when the air chamber is communicated with the air tank group, the diaphragm is pushed by positive pressure to move towards the direction of the liquid chamber.

Wherein the sample analyzer comprises a liquid reservoir and an th reaction cell, and the liquid chamber is connected between the liquid reservoir and the th reaction cell.

The second air storage tank is connected with the fifth air storage tank through a fifth control valve and is used for establishing second negative pressure in the fifth air storage tank through the th negative pressure.

The sample analyzer further comprises a seventh control valve and a second flow limiting member, wherein the seventh control valve is connected between the fifth air storage tank and the second flow limiting member, and the second flow limiting member is used for releasing partial pressure in the fifth air storage tank.

The sample analyzer further comprises a second reaction pool, and the fifth gas storage tank is communicated to an outlet of the second reaction pool.

In another aspect, there is provided a method of driving sample analyzers, the method comprising:

driving an air pump to establish positive pressure and negative pressure in the air storage tank group; and

the positive pressure and the negative pressure drive a flow path of the sample analyzer.

Wherein, the "driving the air pump to establish positive pressure and negative pressure in the air storage tank group" comprises:

the air pump is actuated to establish a th positive pressure in the th reservoir and a th negative pressure in the second reservoir, respectively.

When the absolute value of the pressure of the th positive pressure is smaller than the th threshold value, the air pump is driven to pressurize in the th air storage tank, so that the absolute value of the pressure of the th positive pressure reaches the th threshold value.

When the absolute value of the pressure of the th positive pressure is greater than or equal to a th threshold value and the absolute value of the pressure of the th negative pressure is smaller than a second threshold value, the air pump is driven to pressurize in the second air storage tank, so that the absolute value of the pressure of the th negative pressure reaches the second threshold value.

When the absolute value of the pressure of the th positive pressure is greater than or equal to a th threshold value and less than a third threshold value, and the absolute value of the pressure of the th negative pressure is greater than or equal to a second threshold value, the air pump is driven to build pressure in the th air storage tank, so that the absolute value of the pressure of the th positive pressure reaches the third threshold value.

When the absolute value of the pressure of the th positive pressure is greater than or equal to a third threshold value, and the absolute value of the pressure of the th negative pressure is greater than or equal to a second threshold value and less than a fourth threshold value, the air pump is driven to pressurize in the second air storage tank, so that the absolute value of the pressure of the th negative pressure reaches the fourth threshold value.

When the absolute value of the pressure of the th positive pressure is greater than or equal to a third threshold and less than a fifth threshold, and the absolute value of the pressure of the th negative pressure is greater than or equal to a fourth threshold, the air pump is driven to pressurize the air in the th air tank, so that the absolute value of the pressure of the th positive pressure reaches the fifth threshold.

Wherein the positive pressure establishes a second positive pressure within the third reservoir.

Wherein the second positive pressure urges sheath fluid within the sheath fluid pool into the flow chamber.

Wherein the third air reservoir is disconnected from the th air reservoir before the sheath fluid in the sheath fluid reservoir is pushed by the second positive pressure into the flow chamber.

And when the second positive pressure pushes the sheath liquid in the sheath liquid pool to enter the flowing chamber, the pressure change of the second positive pressure is detected through a third pressure sensor.

Wherein the positive pressure creates a third positive pressure within a fourth tank.

Wherein, the stock solution pond is connected the th reaction tank, the fourth gas holder intercommunication the stock solution pond, for reagent in the stock solution pond gets into the reaction tank provides the drive power.

Wherein the negative pressure creates a second negative pressure within a fifth air tank.

And communicating the fifth gas storage tank to an outlet of the second reaction tank so as to pump out the liquid in the second reaction tank by using the second negative pressure.

Wherein the driving of the air pump to establish a th positive pressure in the th air tank comprises:

the air pump establishes a th positive pressure with an absolute value of the pressure greater than a th preset value in the th air storage tank, and conducts the th air storage tank to the atmosphere, so that the absolute value of the pressure of the th positive pressure is reduced to the th preset value;

and/or the presence of a gas in the gas,

the process of driving the air pump to establish th negative pressure in the second air tank comprises the following steps:

the air pump establishes th negative pressure with the absolute pressure value larger than a second preset value in the second air storage tank, and conducts the second air storage tank to the atmosphere, so that the absolute pressure value of the second negative pressure is reduced to the second preset value;

and/or the presence of a gas in the gas,

the process at which the positive pressure establishes a second positive pressure within the third tank includes:

conducting the th air tank and the third air tank to ensure that the th positive pressure is the pressure in the third air tank so as to form a second positive pressure with the absolute value of the pressure larger than a third preset value;

and/or the presence of a gas in the gas,

the process of the negative pressure creating a second negative pressure within a fifth tank includes:

and conducting the fifth gas storage tank to the atmosphere to reduce the absolute value of the second negative pressure to the fourth preset value.

Wherein the quantitative pump of the sample analyzer comprises a liquid chamber and a gas chamber, the liquid chamber connects a liquid storage tank and an th reaction tank, and the driving method further comprises:

the liquid storage tank is communicated with the positive pressure so as to push liquid in the liquid storage tank into the liquid chamber by utilizing the positive pressure; and

the gas chamber is communicated with the positive pressure so as to push the liquid in the liquid chamber to the th reaction tank by using the positive pressure.

Compared with the prior art, the invention has the following beneficial effects:

the sample analyzer establishes positive pressure and negative pressure in the gas storage tank group through the gas pump, and then uses the positive pressure and the negative pressure in the gas storage tank group as main driving force of the sample analyzer, so that the sample analyzer can replace a large-flow gas pump in the prior art, and the cost and the energy consumption of the sample analyzer are reduced. Meanwhile, the sample analyzer can adopt the small-volume air pump, so that the overall volume of the sample analyzer can be reduced.

Drawings

In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic block diagram of an sample analyzer provided by the present invention.

Fig. 2 is a schematic view of a portion of the sample analyzer shown in fig. 1.

Fig. 3 is a schematic view of another portion of the sample analyzer of fig. 1.

Fig. 4 is a schematic view of a portion of the sample analyzer of fig. 1.

Fig. 5 is a schematic view of still another portion of the sample analyzer of fig. 1.

Fig. 6 is a schematic view of a further portion of the sample analyzer of fig. 1.

Fig. 7 is a schematic view of still another portion of the sample analyzer of fig. 1.

FIG. 8 is a graph of the pressure change in the third reservoir of the sample analyzer of FIG. 1.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only partial embodiments of of the present invention, rather than all embodiments.

Referring to fig. 1, an embodiment of the present invention provides sample analyzers 100. the sample analyzers 100 can be used to perform biological sample analysis, such as blood, urine, etc.

The sample analyzer 100 comprises an air pump 1, an air storage tank group 2, a sampling assembly 3, a reaction assembly 4 and a detection assembly 5, wherein the air pump 1 is used for establishing positive pressure and negative pressure in the air storage tank group 2, the positive pressure and the negative pressure are used for driving the sampling assembly 3 to collect a biological sample, and/or driving the reaction assembly 4 to process the biological sample to form a liquid to be detected, the reaction assembly 4 comprises at least reaction cells, and/or driving the liquid to be detected by the detection assembly 5 to obtain a detection signal.

In this embodiment, the sample analyzer 100 establishes positive pressure and negative pressure in the gas tank set 2 through the air pump 1, and then uses the positive pressure and the negative pressure in the gas tank set 2 as the main driving force of the sample analyzer 100, so as to replace the large flow air pump 1 in the prior art, thereby reducing the cost and energy consumption of the sample analyzer 100. Meanwhile, the sample analyzer 100 can adopt the air pump 1 with a small volume, so that the overall volume of the sample analyzer 100 can be reduced.

It will be appreciated that the air pump 1 and the air reservoir bank 2 can form part of a drive assembly for the sample analyzer 100, the drive assembly can be used to drive various flow paths (including air and fluid paths) and devices within the sample analyzer 100, the sample analyzer 100 further includes a waste disposal assembly for collecting and discharging waste fluid from the sample analyzer 100, the sampling assembly 3 can include a sampler for collecting and dispensing a biological sample.

referring to FIGS. 1 and 2, as an alternative embodiment , the gas tank set 2 includes a 0 th gas tank 21 and a second gas tank 22. the air pump 1 is connected to the th gas tank 21 through a 1 th control valve 23 for establishing a th positive pressure in the th gas tank 21. the th positive pressure is used for providing a main positive pressure driving force for the sample analyzer 100. the th control valve 23 is used for connecting or disconnecting the air pump 1 to the th gas tank 21. the air pump 1 is connected to the second gas tank 22 through a second control valve 24 for establishing a th negative pressure in the second gas tank 22. the th negative pressure is used for providing a main negative pressure driving force for the sample analyzer 100. the second control valve 24 is used for connecting or disconnecting the air pump 1 to the second gas tank 22.

In , the air pump 1 is a single-head pump for pressurizing the th air tank 21 when the th control valve 23 is on and the second control valve 24 is off, and for pressurizing the second air tank 22 when the th control valve 23 is off and the second control valve 24 is on.

In this embodiment, the air pump 1 is a single-head pump for unidirectional pressure buildup, and separately builds pressure for the air storage tank 21 or separately builds pressure for the second air storage tank 22 at the same time of , and the single-head pump for unidirectional pressure buildup has low cost, which is beneficial to further to reduce the cost of the sample analyzer 100.

As will be understood by those skilled in the art, the air pump 1 may also be a single-head pump or a double-head pump, which is used to pressurize the air tank 21 and the second air tank 22 when the control valve 23 is turned on and the second control valve 24 is turned on, in this embodiment, the air pump 1 is a single-head pump or a double-head pump capable of achieving bidirectional pressurization, and is capable of simultaneously pressurizing the air tank 21 and the second air tank 22 at time, so as to increase the pressure buildup speed of the sample analyzer 100, which is beneficial to increase the detection speed of the sample analyzer 100.

referring to FIGS. 1 and 2, as an alternative embodiment , the sample analyzer 100 further includes a controller 6 and a pressure sensor set 7. the pressure sensor set 7 is used for detecting the pressure in the gas storage tank set 2 and feeding back a signal to the controller 6. the controller 6 controls the actions of the gas pump 1, the control valve 23 and the second control valve 24 according to the signal.

The pressure sensor group 7 comprises a plurality of pressure sensors, which can be respectively disposed in a plurality of gas tanks of the gas tank group 2, such as the pressure sensor 71 disposed in the th gas tank 21 and the second pressure sensor 72 disposed in the second gas tank 22.

The controller 6 is used to control both the air pump 1, the th control valve 23, and the second control valve 24, as well as other components in the sample analyzer 100. the controller 6 is capable of controlling the workflow of the sample analyzer 100 and processing the detection signals to form an analysis result.

For example, the th pressure sensor 71 monitors the 0 th positive pressure in the th air tank 21 in real time, when the 1 th positive pressure is insufficient to build the pressure, the controller 6 controls the th control valve 23 to communicate the air pump 1 with the th air tank 21, the air pump 1 operates, the air pump 1 builds the pressure in the th air tank 21 to raise the pressure of the th positive pressure, and when the th pressure sensor 71 detects that the pressure of the th positive pressure is required, the controller 6 controls the th control valve 23 to disconnect the air pump 1 from the th air tank 21, and the air pump 1 stops operating.

The process of the air pump 1 building pressure in the air tank 21 is that the air pump 1 firstly builds a 1 th positive pressure with an absolute value of pressure greater than a 0 th preset value in the th air tank 21, then conducts the 2 th air tank 21 to the atmosphere, so that the absolute value of the pressure of the 3 th positive pressure is reduced to the 4 th preset value, because the initial pressure value of the th positive pressure built in the 5 th air tank 21 by the air pump 1 is greater than the th preset value, even if an overshoot and a rebound phenomenon occurs, the pressure value of the th positive pressure can still keep a state greater than the th preset value, and then the absolute value of the pressure of the th positive pressure is reduced to the th preset value by releasing part of the th positive pressure, so that the th positive pressure has an accurate pressure value after the pressure building process is completed.

The second pressure sensor 72 monitors the th negative pressure in the second air tank 22 in real time, when the th negative pressure is insufficient to build pressure, the controller 6 controls the second control valve 24 to communicate the air pump 1 with the second air tank 22, the air pump 1 operates, the air pump 1 builds pressure in the second air tank 22 to reduce the th negative pressure, and when the second pressure sensor 72 detects that the th negative pressure is required, the controller 6 controls the second control valve 24 to cut off the air pump 1 and the second air tank 22, and the air pump 1 stops operating.

The process of the air pump 1 for building the pressure in the second air storage tank 22 comprises the steps that the air pump 1 firstly builds th negative pressure with the absolute pressure value larger than a second preset value in the second air storage tank 22, then the second air storage tank 22 is conducted to the atmosphere, and the absolute pressure value of the th negative pressure is reduced to the second preset value.

Referring to and fig. 1 and 2, as alternative embodiments, at least pressure-cutoff valves 8 are disposed on the flow path of the sample analyzer 100, and the th positive pressure is used to drive the pressure-cutoff valves 8. the pressure-cutoff valves 8 can be driven by air pressure, such as the th positive pressure, at this time, the pressure-cutoff valves 8 are connected to the th air tanks 21.

In this embodiment, since the internal passage of the pressure-cutoff valve 8 driven by air pressure is smooth, when the pressure-cutoff valve 8 is disposed in a pipeline, contamination of the fluid in the pipeline can be reduced.

In embodiments, the pressure break valve 8 may be disposed between the reaction component 4 and the detection component 5, the liquid to be detected formed by the reaction component 4 enters the detection component 5 through the pressure break valve 8, and the pressure break valve 8 can reduce the pollution to the liquid to be detected, thereby ensuring the accuracy of the detection result of the sample analyzer 100.

In another embodiments, the pressure-cutoff valve 8 can be disposed on the pipeline in the waste liquid treatment assembly, because the fluid flowing in the pipeline in the waste liquid treatment assembly has more impurities, the service life of the common valve is short due to the accumulation and blockage of the impurities, and the pressure-cutoff valve 8 in the embodiment has a smooth internal passage, so the risk of accumulation and blockage of the impurities can be reduced, and the service life is long.

referring to FIGS. 1 and 2, as an alternative embodiment , the sample analyzer 100 further includes a waste liquid tank 91 and a liquid pump 92, wherein the waste liquid tank 91 is connected to the second air container 22, the liquid pump 92 is used for pumping waste liquid in the waste liquid tank 91 and establishing negative pressure in the waste liquid tank 91, and the waste liquid tank 91 and the liquid pump 92 are part of the waste liquid disposal assembly.

In this embodiment, when the second air tank 22 is connected to the waste liquid tank 91, a negative pressure environment is established in the waste liquid tank 91 by using the th negative pressure, and the waste liquid tank 91 pumps the waste liquid in the sample analyzer 100 through its internal negative pressure to realize waste liquid collection, because the waste liquid in the waste liquid tank 91 is pumped out of the machine by the liquid pump 92 to be discharged, there is no need to switch the pressure in the waste liquid tank 91, a negative pressure state can be always maintained in the waste liquid tank 91, so that the waste liquid in the sample analyzer 100 can be continuously pumped through its internal negative pressure by the waste liquid tank 91, and thus the waste liquid collection and waste liquid discharge actions of the waste liquid processing module can be performed in parallel and without interference, the waste liquid processing efficiency of the waste liquid processing module is high, the measurement speed of the sample analyzer 100 is fast, a negative pressure is always maintained in the waste liquid tank 91 without performing positive and negative pressure switching, so that the consumption caused by positive and negative pressure switching can be avoided, and the air pump 1 can better satisfy the requirement of the sample analyzer 100 for driving the air pump 92, and the waste liquid tank 91 can be used for analyzing bubbles in the waste liquid, so that the air pump 22 can be filled into the waste liquid tank 91 or the waste liquid tank 91 for a long time.

It will be appreciated that while the use of the liquid pump 92 to remove waste and assist in pressurizing will effectively reduce the possibility of waste back-flow, various components may fail or the waste tube may become clogged, thus adding a back-flow prevention feature to the sample analyzer 100 to further to reduce the risk of waste back-flow.

In the th embodiment, a th float switch 911 is provided in the waste liquid tank 91 for detecting the liquid level in the waste liquid tank 91, when the th float switch 911 floats, a sensor mounted on the th float switch 911 detects a change in electric potential, indicating that the th float switch 911 has floated in the waste liquid tank 91, if the continuous floating time exceeds a set value, an alarm is given to stop the measurement, thereby preventing the waste liquid from flowing backward into the second gas tank 22.

In a second embodiment, the sample analyzer 100 further comprises a buffer tank 93, the buffer tank 93 is connected between the second air container 22 and the waste liquid tank 91, the buffer tank 93 is used for preventing the waste liquid in the waste liquid tank 91 from flowing back into the second air container 22, and the buffer tank 93 can be liquid containers with low inlet and high outlet.

In the third embodiment, a second float switch 221 is provided in the second air tank 22 to detect the liquid level in the second air tank 22. When the second float switch 221 floats up, the sensor mounted on the second float switch 221 detects the potential change, and if liquid enters the second air storage tank 22, an alarm is given immediately, and the measurement is stopped. Likewise, a float switch for measuring the level of the liquid can also be provided in other gas tanks and/or liquid reservoirs and/or waste liquid reservoirs.

It will be appreciated that the three embodiments described above may also be combined with each other to form a more effective anti-back flow device.

Optionally, a stop valve is arranged between the waste liquid pool 91 and the second air storage tank 22, and is used for communicating or cutting off the waste liquid pool 91 and the second air storage tank 22.

Optionally, the waste liquid pool 91 is connected to the reaction assembly 4, and the waste liquid pool 91 is configured to collect waste liquid of the reaction assembly 4, where at least reaction pools are included in the reaction assembly 4, and the waste liquid pool 91 is connected to an outlet of the reaction pools and configured to collect waste liquid in the reaction pools.

Optionally, a stop valve may be disposed between the waste liquid tank 91 and the liquid pump 92. The shut-off valve may also be modified to be a one-way valve or omitted when the liquid pump 92 has sealing properties.

Optionally, the sample analyzer 100 further includes a second waste liquid pool 94 and a switch 95, the second waste liquid pool 94 is used for collecting waste liquid discharged by positive pressure driving, the switch 95 is connected between the second waste liquid pool 94 and the waste liquid pool 91, and the switch 95 is used for connecting or disconnecting the second waste liquid pool 94 and the waste liquid pool 91. The second waste reservoir 94 has an interface to atmosphere. When the switching member 95 communicates the second waste liquid tank 94 with the waste liquid tank 91, the waste liquid in the second waste liquid tank 94 enters the waste liquid tank 91 under the action of the pressure difference.

referring to FIGS. 1-3, as an alternative embodiment , the tank set 2 further includes a third tank 25. the tank 21 is connected to the third tank 25 via a third control valve 26 for establishing a second positive pressure in the third tank 25 via a th positive pressure, wherein the second positive pressure is equal to or less than the th positive pressure.

The third tank 25 is provided with a third pressure sensor 73 for detecting the pressure in the third tank 25, the controller 6 is connected to the third control valve 26 for controlling the operation of the third control valve 26, the controller 6 controls the third control valve 26 to communicate the third tank 25 with the th tank 21 when the third pressure sensor 73 detects that the second positive pressure in the third tank 25 is insufficient, the th positive pressure increases the pressure of the second positive pressure, and the controller 6 controls the third control valve 26 to block the third tank 25 from the th tank 21 when the third pressure sensor 73 detects that the pressure of the second positive pressure is required.

Optionally, the sample analyzer 100 further includes a sixth control valve 252 and an flow restriction 253, the sixth control valve 252 being connected between the third reservoir 25 and the flow restriction 253, the flow restriction 253 being adapted to relieve a portion of the pressure within the third reservoir 25.

The air tank 21 establishes a second positive pressure in the third air tank 25 through a positive pressure by the third control valve 26 communicating the air tank 21 and the third air tank 25 to make the positive pressure pressurize in the third air tank 25 to form a second positive pressure with an absolute value of pressure greater than a third preset value, the sixth control valve 252 communicating the third air tank 25 with the current-limiting piece 253, the current-limiting piece 253 releases a part of pressure in the third air tank 25 to reduce the absolute value of the second positive pressure to the third preset value.

Optionally, the -th flow restriction 253 is a flow restriction tube, a flow restriction valve, or a flow restriction orifice.

Optionally, the third control valve 26 and/or the sixth control valve 252 are/is a stop valve or a two-position two-way valve.

Optionally, the sample analyzer 100 further comprises a sheath fluid cell 51 and a flow chamber 52, wherein an outlet of the sheath fluid cell 51 is connected to a sheath fluid inlet of the flow chamber 52, the third air reservoir 25 is communicated with the sheath fluid cell 51 for pushing the sheath fluid in the sheath fluid cell 51 to flow into the flow chamber 52, an outlet of the flow chamber 52 is provided with an optical detection component 53 for detecting the number of cells by an optical detection method, the optical detection component 53 may be an part of the detection component 5, and the liquid to be detected entering the flow chamber 52 is detected by the optical detection component 53 under the pressure driving to obtain a detection signal.

In this embodiment, since the second positive pressure in the third air tank 25 can realize accurate pressure buildup through the above pressure buildup process, the second positive pressure can satisfy a preset condition and stably push the sheath fluid into the flow chamber 52, so that the optical detection assembly 53 can obtain an accurate detection result by detecting the liquid to be detected.

Optionally, the controller 6 is coupled to the third control valve 26 for disconnecting the third air reservoir 25 from the air reservoir 21 via the third control valve 26 when sheath fluid in the sheath fluid reservoir 51 flows into the flow chamber 52.

Optionally, the third pressure sensor 73 of the third air tank 25 is further configured to detect the pressure in the third air tank 25 and/or the sheath fluid pool 51 when the third control valve 26 disconnects the third air tank 21 from the third air tank 25 and the sheath fluid in the sheath fluid pool 51 flows to the flow chamber 52.

In this embodiment, since the air tank 21 does not supplement the second positive pressure in the third air tank 25 any more, and the second positive pressure is continuously decreased when the sheath fluid is driven, the change in the second positive pressure detected by the third pressure sensor 73 can accurately feed back the flowing state of the sheath fluid, so as to provide a reliable reference for the detection result of the optical detection module 53, and thus the detection result provided by the sample analyzer 100 is reliable.

referring to FIGS. 1, 2 and 4, as an alternative embodiment , the tank set 2 further includes a fourth tank 27, and the tank 21 is connected to the fourth tank 27 through a fourth control valve 28 for establishing a third positive pressure in the fourth tank 27 through a positive pressure, wherein the third positive pressure is positive pressure.

The fourth air tank 27 is provided with a fourth pressure sensor 74 for detecting the pressure in the fourth air tank 27, the controller 6 is connected to the fourth control valve 28 for controlling the operation of the fourth control valve 28, when the fourth pressure sensor 74 detects that the pressure of the third positive pressure in the fourth air tank 27 is insufficient, the controller 6 controls the fourth control valve 28 to communicate the fourth air tank 27 with the air tank 21, the is normally pressing the fourth air tank 27 to increase the pressure of the third positive pressure, and when the fourth pressure sensor 74 detects that the pressure of the third positive pressure reaches a requirement, the controller 6 controls the fourth control valve 28 to cut off the fourth air tank 27 from the air tank 21.

Optionally, the sample analyzer 100 comprises a liquid storage tank 41 and an -th reaction tank 42, wherein the liquid storage tank 41 is connected with the -th reaction tank 42, the fourth gas storage tank 27 is communicated with the liquid storage tank 41 to provide driving force for the reagent in the liquid storage tank 41 to enter the -th reaction tank 42, the liquid storage tank 41 can be used for storing reagents such as diluent, hemolytic agent or dye, and the -th reaction tank 42 can be a reaction tank for processing a biological sample to form a test solution for detecting hemoglobin count, a reaction tank for processing a biological sample to form a test solution for detecting leukocyte count (and/or nucleated red blood cell classification and/or basophil classification), a reaction tank for processing a biological sample to form a test solution for detecting leukocyte classification, a reaction tank for processing a biological sample to form a test solution for detecting red blood cell count, or a reaction tank for processing a biological sample to form a test solution for detecting reticulocyte count.

referring to FIGS. 1, 2 and 5, as an alternative embodiment, the sample analyzer 100 further includes a constant displacement pump 43, wherein the constant displacement pump 43 has a diaphragm 431 and a liquid chamber 432 and a gas chamber 433 disposed on both sides of the diaphragm 431, the constant displacement pump 43 is connected to the gas tank set 2, and the gas tank set 2 can provide the positive pressure to the constant displacement pump 43. for example, the constant displacement pump 43 is connected to the fourth gas tank 27 in the gas tank set 2, and the fourth gas tank 27 provides the third positive pressure to the constant displacement pump 43. when the liquid chamber 432 is connected to the gas tank set 2, the positive pressure pushes the diaphragm 431 to move in the direction of the gas chamber 433, when the gas chamber 433 is connected to the gas tank set 2, the positive pressure pushes the diaphragm 431 to move in the direction of the liquid chamber 432. when the diaphragm 431 moves in the direction of the gas chamber 433, the liquid chamber 432 increases in volume, the liquid enters the liquid chamber 432, and when the constant displacement pump 43 completes, when the diaphragm 431 moves in the direction of the liquid chamber 432, the liquid chamber 432 decreases in volume, and the liquid discharge from the liquid chamber 432.

In this embodiment, the liquid suction action and the liquid discharge action of the fixed displacement pump 43 are both completed by the positive pressure driving, that is, the fixed displacement pump 43 adopts a bidirectional positive pressure driving manner, so that the driving difficulty is low, which is beneficial to reducing the gas consumption of the gas storage tank group 2, thereby reducing the energy consumption of the sample analyzer 100. Meanwhile, since the constant delivery pump 43 does not need to be driven by negative pressure, the sample analyzer 100 can accurately control the positive pressure environment, thereby facilitating stable control of the operation of the constant delivery pump 43 and avoiding unstable liquid suction and discharge operations of the constant delivery pump 43 due to unstable negative pressure environment.

In another embodiments, the fixed displacement pump 43 may be connected to the air tank 21, and the air tank 21 may provide the positive pressure to the fixed displacement pump 43. in yet another embodiments, the fixed displacement pump 43 may be connected to the third air tank 25, and the third air tank 25 may provide the second positive pressure to the fixed displacement pump 43.

Alternatively, the sample analyzer 100 may include a liquid reservoir 41 and an -th reaction cell 42, the liquid chamber 432 may be connected between the liquid reservoir 41 and the -th reaction cell 42, the dosing pump 43 may quantitatively feed the reagent in the liquid reservoir 41 into the -th reaction cell 42, the dosing pump 43 may further provide a spare reagent for the -th reaction cell 42 when the reagent in the liquid reservoir 41 is insufficient, so that the -th reaction cell 42 may be continuously supplied with the liquid, thereby increasing the detection speed of the sample analyzer 100, the sample analyzer 100 may further include a control valve 45, the control valve 45 may connect the liquid reservoir 41, the -th reaction cell 42, and the liquid chamber 432, for communication and disconnection, for example, the control valve 45 may connect the liquid reservoir 41 with the liquid chamber 432 and disconnect the -th reaction cell 42 from the liquid chamber 432, the liquid in the liquid reservoir 41 may enter the liquid chamber 432, the dosing pump 43, or the control valve 45 may connect the liquid suction valve 45 with the liquid reservoir 41 and disconnect the liquid chamber from the liquid chamber 432, the liquid chamber 432 may enter the liquid chamber 3542, the liquid chamber 432.

In embodiments, the th reaction cell 42 is a reaction cell for processing a biological sample to form a solution to be tested for hemoglobin count, a reaction cell for processing a biological sample to form a solution to be tested for leukocyte count (and/or nucleated red blood cell classification and/or basophil classification), a reaction cell for processing a biological sample to form a solution to be tested for leukocyte count, a reaction cell for processing a biological sample to form a solution to be tested for red blood cell count, or a reaction cell for processing a biological sample to form a solution to be tested for reticulocyte count.

In , the reservoir 41 is used to store diluent, the liquid chamber 432 is used to store diluent, and the dosing pump 43 can temporarily supply diluent to the th reaction cell 42 when the diluent in the reservoir 41 is insufficient, so as to ensure that the diluent is supplied to the th reaction cell 42 without interruption, thereby increasing the detection speed of the sample analyzer 100.

Specifically, the method comprises the following steps:

when the control valve 45 communicates the liquid reservoir 41 with the liquid chamber 432, the diluent in the liquid reservoir 41 enters the liquid chamber 432 to form a standby diluent, when the -th reaction tank 42 requires the diluent, if the diluent in the liquid reservoir 41 is sufficient, the control valve 45 communicates the liquid reservoir 41 with the -th reaction tank 42, and the diluent in the liquid reservoir 41 enters the -th reaction tank 42. if the diluent in the liquid reservoir 41 is insufficient, the control valve 45 disconnects the liquid reservoir 41 from the -th reaction tank 42 and communicates the liquid reservoir 432 with the -th reaction tank 42, and the standby diluent in the liquid reservoir 432 enters the -th reaction tank 42 to continuously supply the diluent to the -th reaction tank 42. at this time, the liquid reservoir 41 may timely draw the diluent from the reagent barrel to supplement the diluent, for example, the liquid reservoir 41 communicates with the second gas holder 22 to draw the diluent from the standby reagent barrel by using the negative pressure of the . when the liquid reservoir 41 is connected with the other reagent barrel, the diluent may be drawn directly from the liquid reservoir 3542.

, referring to fig. 1, 2, 6 and 7, as alternative embodiments, the gas tank group 2 further includes a fifth gas tank 29, and the second gas tank 22 is connected to the fifth gas tank 29 through a fifth control valve 210, and is configured to establish a second negative pressure in the fifth gas tank 29 through the th negative pressure, wherein an absolute value of the second negative pressure is less than or equal to an absolute value of the th negative pressure.

A fifth pressure sensor 75 is disposed in the fifth air tank 29, and is configured to detect a pressure within the fifth air tank 29. The controller 6 is connected to the fifth control valve 210, and is configured to control the operation of the fifth control valve 210. When the fifth pressure sensor 75 detects that the pressure of the second negative pressure in the fifth air tank 29 is insufficient, the controller 6 controls the fifth control valve 210 to communicate the fifth air tank 29 with the second air tank 22, and the second negative pressure builds up in the fifth air tank 29 to reduce the pressure of the second negative pressure. When the fifth pressure sensor 75 detects that the pressure of the second negative pressure reaches the requirement, the controller 6 controls the fifth control valve 210 to cut off the fifth air tank 29 and the second air tank 22.

Optionally, the sample analyzer 100 further comprises a seventh control valve 292 and a second flow restriction 293, wherein the seventh control valve 292 is connected between the fifth air reservoir 29 and the second flow restriction 293. The second flow restriction 293 is used for releasing a part of the pressure in the fifth air tank 29.

The process of the second air storage tank 22 establishing the second negative pressure in the fifth air storage tank 29 by the second negative pressure is as follows: the fifth control valve 210 connects the second air tank 22 and the fifth air tank 29, so that the second negative pressure is built in the fifth air tank 29 to form a second negative pressure with an absolute value of pressure greater than a fourth preset value; the seventh control valve 292 connects the fifth air tank 29 and the second flow limiting member 293, and the second flow limiting member 293 releases a part of the pressure in the fifth air tank 29, so that the absolute value of the second negative pressure is increased to the fourth preset value. The pressure building process can eliminate two phenomena of overshoot and rebound, and accurate pressure building is achieved.

Optionally, the second flow restriction 293 is a flow restriction pipe, a flow restriction valve or a flow restriction hole.

Optionally, the fifth control valve 210 and/or the seventh control valve 292 are/is a stop valve or a two-position two-way valve.

Optionally, the sample analyzer 100 further comprises a second reaction cell 44, and the fifth gas storage tank 29 is connected to an outlet of the second reaction cell 44. When the outlet of the second reaction tank 44 is connected to the fifth gas container 29, the liquid in the second reaction tank 44 flows into the fifth gas container 29 under the driving of the second negative pressure.

For example, the second reaction cell 44 is provided with an impedance detection element 54 at the outlet thereof for detecting the number of red blood cells by an impedance method (coulter principle), the second reaction cell 44 is a reaction cell for processing a biological sample to form a liquid to be detected for detecting the red blood cell count, the impedance method employs a time-measurement method for quantification (i.e. a statistical volume is equal to a flow rate through a detection aperture x a statistical time), and since the statistical time is fixed, the flow rate through the detection aperture determines a detection volume and directly affects the measurement result, the impedance detection element 54 is part of the detection element 5, the sample analyzer 100 drives the liquid to be detected in the second reaction cell 44 through the impedance detection element 54 by establishing a stable and accurate second negative pressure in the fifth gas storage tank 29, so that the liquid to be detected in the second reaction cell 44 is detected by the impedance detection element 54 to obtain a detection signal, and thus the flow rate of the liquid to be detected through the impedance detection element 54 is stable, so that the impedance detection element 54 can more accurately and reliably detect the detection result of the liquid to be detected.

In embodiments, as shown in FIG. 7, the fifth control valve 210 is directly connected to the second reservoir 22. in another embodiments, the fifth control valve 210 is connected to the second reservoir 22 via the waste pool 91 (and the buffer pool 93). The second reservoir 22 is connected to the waste pool 91, and the waste pool 91 has the same or similar pressure as the second reservoir 22, i.e., the pressure in the waste pool 91 is at or near the th negative pressure or the th negative pressure, at which time, the second negative pressure can be established in the fifth reservoir 29 by the pressure in the waste pool 91. the waste pool 91 is also used for collecting waste liquid in the fifth reservoir 29. the waste liquid in the waste pool 91 can be discharged by the liquid pump 92.

Referring to fig. 1 to 8, , an embodiment of the present invention further provides a method for driving sample analyzers 100, which can be applied to the sample analyzers 100 described in the above embodiments.

The driving method includes:

driving the air pump 1 to establish positive pressure and negative pressure in the air storage tank group 2; and

the positive pressure and the negative pressure drive the flow path of the sample analyzer 100.

In this embodiment, the driving method establishes positive pressure and negative pressure in the gas tank group 2 through the air pump 1, and then uses the positive pressure and the negative pressure in the gas tank group 2 as the main driving force of the sample analyzer 100, so as to replace the large-flow air pump 1 in the prior art, thereby reducing the cost and energy consumption of the sample analyzer 100. Meanwhile, since the driving method can use the air pump 1 having a small volume, the overall volume of the sample analyzer 100 can be reduced.

As an alternative to , the step of driving the air pump 1 to create positive and negative pressures in the air tank set 2 includes driving the air pump 1 to create a th positive pressure in the th air tank 21 and a th negative pressure in the second air tank 22, respectively.

The action of the air pump 1 to establish the th positive pressure and the action of the th negative pressure can be performed in a staggered manner or simultaneously, the th positive pressure is used for providing main positive pressure driving force for the sample analyzer 100, and the th negative pressure is used for providing main negative pressure driving force for the sample analyzer 100.

Optionally, the driving the air pump 1 to establish a th positive pressure in the th air storage tank 21 includes:

the air pump 1 establishes a th positive pressure having an absolute value of pressure greater than a th preset value in the th air storage tank 21, and

and (3) conducting the st air tank 21 to the atmosphere, so that the absolute value of the pressure of the th positive pressure is reduced to the th preset value.

In this embodiment, the process of establishing th positive pressure can eliminate two phenomena of overshoot and rebound, and realize accurate pressure establishment.

Optionally, the process of driving the air pump 1 to establish the th negative pressure in the second air storage tank 22 includes:

the air pump 1 creates th negative pressure in the second air storage tank 22, the absolute value of the pressure of which is greater than the second preset value, and

and conducting the second air storage tank 22 to the atmosphere, so that the absolute pressure value of the second negative pressure is reduced to the second preset value.

In the embodiment, the th negative pressure is established, so that two phenomena of overshoot and rebound can be eliminated, and accurate pressure establishment can be realized.

As an alternative , when the air pump 1 of the sample analyzer 100 uses a low-cost single-head pump with unidirectional pressure buildup for pressure buildup, the air pump 1 needs to separately pressurize the st air tank 21 and the second air tank 22, and the pressure buildup principle of the st air tank 21 and the second air tank 22 is as follows:

the pressure of the positive pressure P1 in the air storage tank 21 is divided into three pressure levels, that is, a threshold A1, a third threshold A2 and a fifth threshold A3, the threshold A1 is smaller than the third threshold A2(A1 < A2), the third threshold A2 is smaller than the fifth threshold A3(A2 < A3), the pressure of the negative pressure P2 in the second air storage tank 22 is divided into two pressure levels, that is, a second threshold B1 and a fourth threshold B2, and the second threshold B1 is smaller than the fourth threshold B2(B1 < B2).

When the absolute value of the pressure of the th positive pressure P1 is smaller than the th threshold A1(| P1| < A1), the priority is highest, the positive pressure is established at this time, and when the pressure is established to reach the th threshold A1(| P1| ≧ A1), the pressure establishing process is ended.

When the absolute value of the actual pressure of the th negative pressure P2 is smaller than the second threshold B1(| P2| < B1), the priority is the second highest, the negative pressure is established at this time, and when the pressure is established to reach the second threshold B1(| P2| ≧ B1), the pressure establishing process is ended.

When the absolute value of the pressure of the th positive pressure P1 is greater than or equal to the th threshold A1 and less than the third threshold A2(A1 ≦ P1| < A2), the priority is third high, the positive pressure is established, and when the pressure is established to reach the third threshold A2(P1| ≧ A2), the pressure building process is ended.

When the absolute value of the pressure of the th negative pressure P2 is greater than or equal to the second threshold value B1 and less than the fourth threshold value B2(B1 ≦ P2| < B2), the priority level is fourth high, the negative pressure is established, and when the pressure is established to reach the fourth threshold value B2(| P2| ≧ B2), the pressure establishment process is ended.

When the absolute value of the pressure of the th positive pressure P1 is greater than or equal to the third threshold value A2 and less than the fifth threshold value A3(A2 ≦ P1| < A3), the priority is fifth high, the positive pressure is established, and when the pressure is established to reach the fifth threshold value A3(P1| ≧ A3), the pressure establishment process is ended.

In other words:

when the absolute value of the pressure of the th positive pressure P1 is smaller than the th threshold a1(| P1| < a1), the air pump 1 is driven to pressurize in the th air storage tank 21, so that the absolute value of the pressure of the th positive pressure P1 reaches the th threshold a1(| P1| ≧ a 1).

When the absolute value of the pressure of the -th positive pressure P1 is greater than or equal to a -th threshold value a1(| P1| ≧ a1) and the absolute value of the pressure of the -th negative pressure P2 is less than a second threshold value B1(| P2| < B1), the air pump 1 is driven to build pressure in the second air storage tank 22, so that the absolute value of the pressure of the -th negative pressure P2 reaches the second threshold value B1(| P2| ≧ B1).

When the absolute value of the pressure of the th positive pressure P1 is equal to or greater than a th threshold A1 and less than a third threshold A2(A1 ≦ P1| < A2), and the absolute value of the pressure of the th negative pressure P2 is equal to or greater than a second threshold B1(B1 ≦ P2|), the air pump 1 is driven to build the pressure in the th air storage tank 21, so that the absolute value of the pressure of the th positive pressure P1 reaches the third threshold A2(P1| ≧ A2).

When the absolute value of the pressure of the th positive pressure P1 is equal to or greater than a third threshold value a2(a2 ≦ P1|), and the absolute value of the pressure of the th negative pressure P2 is equal to or greater than a second threshold value B1 and less than a fourth threshold value B2(B1 ≦ P2| < B2), the air pump 1 is driven to pressurize the second air tank 22, so that the absolute value of the pressure of the th negative pressure P2 reaches the fourth threshold value B2(| P2| ≧ B2).

When the absolute value of the pressure of the positive pressure P1 is greater than or equal to a third threshold A2 and smaller than a fifth threshold A3(A2 ≦ P1| < A3), and the absolute value of the pressure of the negative pressure P2 is greater than or equal to a fourth threshold B2(| P2| ≧ B2), the air pump 1 is driven to build pressure in the air storage tank 21, so that the absolute value of the pressure of the positive pressure P1 reaches the fifth threshold A3(P1| ≧ A3).

In this embodiment, the driving method reasonably and skillfully sets the timing and degree of the air pump 1 to establish the positive pressure of the st air tank and the negative pressure of the th air tank according to the urgency of the sample analyzer 100 to the requirements of the positive pressure of the th air tank and the negative pressure of the th air tank, so that the positive pressure of the th air tank and the negative pressure of the th air tank can better realize the driving effect.

Optionally, the sample analyzer 100 further includes a waste liquid tank 91 and a liquid pump 92, the waste liquid tank 91 is connected to the second air storage tank 22, and the liquid pump 92 is configured to draw waste liquid in the waste liquid tank 91 and establish negative pressure in the waste liquid tank 91.

The pressurizing action of the liquid pump 92 and the pressurizing action of the air pump 1 are independent of each other. The rules for the liquid pump 92 to drain waste liquid and establish negative pressure are as follows:

the pressure of the negative pressure P2 in the second air tank 22 further includes two pressure levels, i.e., a sixth threshold B3 and a seventh threshold B4, wherein the fourth threshold B2 is smaller than the sixth threshold B3(B2 < B3), and the sixth threshold B3 is smaller than the seventh threshold B4(B3 < B4).

When the absolute value of the pressure of the th negative pressure P2 is less than the sixth threshold value B3 (| P2| < B3), the liquid pump 92 is started so that the liquid pump 92 discharges the waste liquid in the waste liquid tank 91 and establishes a negative pressure in the waste liquid tank 91, and

when the absolute value of the pressure of the th negative pressure is greater than or equal to the seventh threshold value B4 (| P2| ≧ B4), the liquid pump 92 is turned off.

In this embodiment, since the fourth threshold is smaller than the sixth threshold, the liquid pump 92 is in a state of discharging waste liquid and assisting pressure build-up most of the time, the liquid pump 92 can discharge the waste liquid in the waste liquid tank 91 in time, and the interior of the waste liquid tank 91 is almost in an empty state, so that the waste liquid, bubbles, or the like can be prevented from flowing backward into the second air tank 22 and the air pump 1, and the sample analyzer 100 can normally operate for a long time. Since the liquid pump 92 can assist in establishing the negative pressure, it is possible to solve the problem that the negative pressure flow rate may be insufficient in the air pump 1 of a small flow rate. Since the waste liquid in the waste liquid tank 91 is pumped out of the machine by the liquid pump 92 and discharged, the pressure in the waste liquid tank 91 does not need to be switched, and the waste liquid tank 91 can always maintain a negative pressure state, so that the waste liquid in the sample analyzer 100 can be continuously pumped out by the waste liquid tank 91 through the internal negative pressure thereof, and thus the waste liquid collecting action and the waste liquid discharging action of the waste liquid treatment assembly can be performed in parallel without mutual interference, the waste liquid treatment efficiency of the waste liquid treatment assembly is high, and the overall measurement speed of the sample analyzer 100 is high. The waste liquid pool 91 is always kept at negative pressure, and positive-negative pressure switching is not needed, so that the increase of air consumption caused by positive-negative pressure switching can be avoided, and the air pump 1 with small flow can better meet the driving requirement of the sample analyzer 100.

As an alternative embodiment of , the positive pressure creates a second positive pressure within the third reservoir 25, the second positive pressure being equal to or less than the positive pressure.

Alternatively, the process of "the positive pressure establishing the second positive pressure within the third air reservoir 25" includes:

conducting the st air tank 21 and the third air tank 25 to make the th positive pressure build-up in the third air tank 25 to form a second positive pressure with an absolute value of pressure greater than a third preset value, and

and communicating the third air storage tank 25 to the atmosphere, so that the absolute pressure value of the second positive pressure is reduced to the third preset value.

In this embodiment, the process of establishing the second positive pressure can eliminate two phenomena of overshoot and rebound, and realize accurate pressure establishment. As shown in fig. 8, the line segment 01, the line segment 02, and the line segment 03 in fig. 8 represent the variation process of the second positive pressure, and as can be seen from fig. 8, the above-mentioned pressure-building method may accurately build the second positive pressure at the third preset value P.

Optionally, when the second positive pressure is lower than the third preset value, the th air tank 21 and the third air tank 25 are conducted, so that the second positive pressure reaches the third preset value.

alternative embodiment, the second positive pressure pushes the sheath fluid in the sheath fluid cell 51 into the flow chamber 52. the driving method utilizes the precise second positive pressure to push the sheath fluid, which is beneficial to obtain accurate detection results by the optical detection component 53 disposed in the flow chamber 52. the sheath fluid cell 51 can be connected to the third air reservoir 25, so that the pressure in the sheath fluid cell 51 is the same as the third air reservoir.

Alternatively, the third air tank 25 and the air tank 21 may be disconnected before the sheath fluid in the sheath fluid pool 51 is pushed by the second positive pressure into the flow chamber 52.

In this embodiment, since the third air tank 25 and the air tank 21 are disconnected, the air tank 21 does not supplement the second positive pressure in the third air tank 25, so that the fluctuation range of the second positive pressure is small, the sheath fluid can be stably driven by the second positive pressure, and the optical detection module 53 can obtain an accurate detection result, it can be understood that the sheath fluid pool 51 and the flow chamber 52 can be disconnected by a stop valve when the third air tank 25 is communicated with the air tank 21 for pressure buildup, and the stop valve is opened after the third air tank 25 is disconnected from the air tank 21, so that the sheath fluid in the sheath fluid pool 51 can be pushed by the second positive pressure to enter the flow chamber 52.

Alternatively, the third pressure sensor 73 detects a change in the pressure of the second positive pressure when the sheath fluid in the sheath fluid tank 51 is pushed into the flow chamber 52 by the second positive pressure, since the air tank 21 does not supplement the second positive pressure in the third air tank 25, and the second positive pressure is continuously decreased when the sheath fluid is driven, the change in the second positive pressure detected by the third pressure sensor 73 can accurately feed back the flow state of the sheath fluid, and thus a reliable reference can be provided for the detection result of the optical detection unit 53, and the detection result provided by the sample analyzer 100 is reliable.

Optionally, when the pressure change of the second positive pressure does not meet a preset condition, an alarm is given. If the pressure variation of the second positive pressure does not satisfy the preset condition, the action of the second positive pressure pushing the sheath fluid in the sheath fluid pool 51 to flow out is unstable, and the accuracy of the detection result of the sample analyzer 100 is directly affected. At the moment, the driving method gives an alarm and can remind the user that the corresponding detection result is inaccurate. If the pressure change of the second positive pressure meets a preset condition, the detection result of the sample analyzer 100 is accurate. Therefore, the sample analyzer 100 to which the driving method is applied can provide reliable detection results.

Optionally, the driving method may perform corresponding maintenance (cleaning or pressure reestablishment, etc.) on the sample analyzer 100 after the alarm, so as to ensure the accuracy of the detection result of the next detection.

In , a pressure curve is formed according to the pressure change, and when the slope of the pressure curve is not within a preset range, the pressure change is determined not to satisfy a preset condition, when the slope of the pressure curve is within a preset range, the pressure change is determined to satisfy a preset condition, and the action of the second positive pressure pushing the sheath fluid in the sheath fluid pool 51 to flow out is stable.

In another embodiments, a pressure value data set is formed according to the pressure variation, and when the difference between the data in the pressure value data set is not within a preset range, the pressure variation is determined not to satisfy a preset condition, and when the difference between the data in the pressure value data set is within a preset range, the pressure variation is determined to satisfy a preset condition, and the action of the second positive pressure pushing the sheath fluid in the sheath fluid pool 51 to flow out is stable.

As an alternative embodiment of , the positive pressure creates a third positive pressure within the fourth reservoir 27, the third positive pressure being equal to or less than the positive pressure.

Optionally, the liquid storage tank 41 is connected to the th reaction tank 42, and the fourth gas storage tank 27 is connected to the liquid storage tank 41, so as to push the reagent in the liquid storage tank 41 into the th reaction tank 42 by using the third positive pressure.

Optionally, when the third positive pressure is lower than a fifth preset value, the th air tank 21 and the fourth air tank 27 are conducted, so that the third positive pressure reaches the fifth preset value.

As an alternative , the th negative pressure creates a second negative pressure in the fifth air tank 29, the absolute value of the pressure of the second negative pressure is smaller than or equal to the absolute value of the pressure of the th negative pressure.

Alternatively, the process of "the negative pressure establishing the second negative pressure in the fifth air tank 29" includes:

communicating said fifth reservoir 29 with said second reservoir 22 to cause said th negative pressure to build up pressure within said fifth reservoir 29 to form a second negative pressure having an absolute value greater than a fourth predetermined value, and

and the fifth air storage tank 29 is communicated to the atmosphere, so that the absolute pressure value of the second negative pressure is reduced to the fourth preset value.

In the embodiment, the process of establishing the second negative pressure can eliminate two phenomena of overshoot and rebound, and accurate pressure establishment is realized.

Optionally, when the second negative pressure is lower than a fourth preset value, the second air tank 22 and the fifth air tank 29 are conducted, so that the second negative pressure reaches the fourth preset value.

Optionally, the fifth gas storage tank 29 is conducted to the outlet of the second reaction tank 44, so as to draw the liquid in the second reaction tank 44 by using the second negative pressure. When the outlet of the second reaction tank 44 is connected to the fifth gas container 29, the liquid in the second reaction tank 44 flows into the fifth gas container 29 under the driving of the second negative pressure.

In this embodiment, an impedance detecting unit 54 may be provided at the outlet of the second reaction cell 44 for detecting the number of red blood cells by an impedance method (coulter principle). Because the second negative pressure is accurately built, when the second negative pressure drives the liquid to be detected in the second reaction tank 44 to pass through the impedance detection assembly 54, the flow of the liquid to be detected passing through the impedance detection assembly 54 is stable, and therefore the detection result of the liquid to be detected by the impedance detection assembly 54 is more accurate and reliable.

As alternative embodiments, the quantitative pump 43 of the sample analyzer 100 includes a liquid chamber 432 and a gas chamber 433, the liquid chamber 432 connects the liquid reservoir 41 and the reaction reservoir, the driving method further includes:

the liquid reservoir 41 communicates with the positive pressure to push the liquid in the liquid reservoir 41 into the liquid chamber 432 by the positive pressure; and

the gas chamber 433 is communicated with the positive pressure to push the liquid in the liquid chamber 432 toward the reaction cell by the positive pressure.

In this embodiment, the liquid sucking action (the liquid in the liquid storage tank 41 enters the liquid chamber 432) and the liquid discharging action (the liquid in the liquid chamber 432 flows to the reaction tank) of the metering pump 43 are both driven by the positive pressure (for example, the th positive pressure, the second positive pressure or the third positive pressure), that is, the metering pump 43 adopts a bidirectional positive pressure driving manner, which is less difficult to drive and is beneficial to reducing the gas consumption of the gas tank group 2, thereby reducing the energy consumption of the sample analyzer 100. meanwhile, since the metering pump 43 does not need negative pressure driving, the sample analyzer 100 can realize accurate control of a positive pressure environment, thereby being beneficial to stably controlling the action of the metering pump 43 and avoiding unstable liquid sucking action and liquid discharging action of the metering pump 43 due to unstable negative pressure environment.

While the embodiments of the present invention have been described in detail, the principles and embodiments of the present invention have been illustrated and described herein by means of specific examples, which are provided only for the purpose of facilitating understanding of the method and the core concept of the present invention, and meanwhile, for those skilled in the art , the description should not be construed as limiting the present invention in view of the above description.

Claims (41)

1. sample analyzer, characterized by that, includes air pump, gas holder group, sampling subassembly, reaction unit and determine module, the air pump is used for establishing malleation and negative pressure in the gas holder group, malleation with the negative pressure is used for:

   driving the sampling assembly to collect a biological sample;

   and/or driving the reaction assembly to process the biological sample to form a liquid to be detected, wherein the reaction assembly comprises at least reaction cells;

   and/or driving the liquid to be detected by the detection assembly to obtain a detection signal.
2. The sample analyzer of claim 1 wherein the set of gas tanks includes a th gas tank and a second gas tank, the air pump is connected to the th gas tank through a th control valve for establishing a th positive pressure in the th gas tank, and the air pump is connected to the second gas tank through a second control valve for establishing a th negative pressure in the second gas tank.
3. The sample analyzer of claim 2 wherein the air pump is a single head pump for pressurizing the air reservoir when the th control valve is ON and the second control valve is OFF, and for pressurizing the second air reservoir when the th control valve is OFF and the second control valve is ON.
4. The sample analyzer of claim 2 wherein the air pump is a single head pump or a dual head pump for pressurizing the th and second air reservoirs when the th control valve is open and the second control valve is open.
5. The sample analyzer of claim 2 further comprising a controller and a set of pressure sensors for sensing the pressure within the set of gas reservoirs and feeding back signals to the controller, the controller controlling the operation of the gas pump, the control valve, and the second control valve in response to the signals.
6. The sample analyzer of any of claims 2-5, wherein the sample analyzer has at least pressure relief valves in the flow path, and the th positive pressure is used to actuate the pressure relief valves.
7. The sample analyzer of claim 2 further comprising a waste reservoir coupled to the second reservoir and a liquid pump for drawing waste from the waste reservoir.
8. The sample analyzer of claim 7 wherein the waste reservoir has an th float switch disposed therein for detecting a level of liquid in the waste reservoir.
9. The sample analyzer of claim 7 further comprising a buffer cell connected between the second reservoir and the waste reservoir, the buffer cell configured to prevent waste in the waste reservoir from flowing back into the second reservoir.
10. The sample analyzer of claim 7 wherein a second float switch is disposed within the second reservoir for detecting a level of liquid within the second reservoir.
11. The sample analyzer of claim 2 further comprising a waste reservoir and a liquid pump for drawing waste within the waste reservoir and establishing a negative pressure within the waste reservoir.
12. The sample analyzer of any of claims 7-11, wherein the waste reservoir is connected to the reaction module and is configured to collect waste from the reaction module.
13. The sample analyzer of claim 5 wherein the set of gas tanks further includes a third gas tank, the gas tank being connected to the third gas tank by a third control valve for establishing a second positive pressure within the third gas tank by a positive pressure.
14. The sample analyzer of claim 13 further including a sixth control valve connected between the third reservoir and the flow restrictor and an flow restrictor, the flow restrictor being configured to relieve some of the pressure in the third reservoir.
15. The sample analyzer of claim 13 or 14 further comprising a sheath fluid reservoir and a flow chamber, wherein an outlet of the sheath fluid reservoir is connected to a sheath fluid inlet of the flow chamber, and wherein the third gas reservoir is in communication with the sheath fluid reservoir for urging sheath fluid in the sheath fluid reservoir into the flow chamber.
16. The sample analyzer of claim 15 wherein the controller is coupled to the third control valve for disconnecting the third air reservoir from the air reservoir through the third control valve when sheath fluid within the sheath fluid reservoir flows into the flow chamber.
17. The sample analyzer of claim 15 wherein the pressure sensor set further comprises a third pressure sensor connected to sense pressure within the third gas reservoir and/or the sheath fluid when the third control valve disconnects the third gas reservoir from the third gas reservoir and sheath fluid within the sheath fluid flows to the flow chamber.
18. The sample analyzer of claim 5 wherein the set of gas tanks further includes a fourth gas tank, the gas tank being connected to the fourth gas tank by a fourth control valve for establishing a third positive pressure within the fourth gas tank by a positive pressure.
19. The sample analyzer of claim 18, wherein the sample analyzer comprises a reservoir and an th reaction cell, the reservoir is connected to the th reaction cell, and the fourth gas container is connected to the reservoir for pushing the reagent in the reservoir into the th reaction cell.
20. The sample analyzer of claim 1 or 18, further comprising a quantitative pump having a diaphragm, a liquid chamber and an air chamber, the liquid chamber and the air chamber being located on opposite sides of the diaphragm, wherein the quantitative pump is connected to the set of air tanks, the positive pressure pushes the diaphragm to move in a direction toward the air chamber when the liquid chamber is connected to the set of air tanks, and the positive pressure pushes the diaphragm to move in a direction toward the liquid chamber when the air chamber is connected to the set of air tanks.
21. The sample analyzer of claim 20 wherein the sample analyzer comprises a liquid reservoir and an th reaction cell, the liquid reservoir being connected between the liquid reservoir and the th reaction cell.
22. The sample analyzer of claim 5 wherein the set of gas tanks further includes a fifth gas tank, the second gas tank connected to the fifth gas tank by a fifth control valve for establishing a second negative pressure within the fifth gas tank via the th negative pressure.
23. The sample analyzer of claim 22 further comprising a seventh control valve connected between the fifth reservoir and the second flow restriction, and a second flow restriction for relieving a portion of the pressure within the fifth reservoir.
24. The sample analyzer of claim 22 or 23 further comprising a second reaction cell, the fifth gas reservoir being connected to an outlet of the second reaction cell.
25. A method of driving a sample analyzer, the method comprising:

    driving an air pump to establish positive pressure and negative pressure in the air storage tank group; and

    the positive pressure and the negative pressure drive a flow path of the sample analyzer.
26. The driving method according to claim 25, wherein said driving the air pump to establish positive and negative pressures in the air tank group comprises:

    the air pump is actuated to establish a th positive pressure in the th reservoir and a th negative pressure in the second reservoir, respectively.
27. The driving method as claimed in claim 26, wherein when the absolute value of the pressure of the th positive pressure is smaller than the th threshold, the air pump is driven to pressurize the th air tank, so that the absolute value of the pressure of the th positive pressure reaches the th threshold.
28. The driving method as claimed in claim 26, wherein when the absolute value of the pressure of the th positive pressure is equal to or greater than a th threshold value and the absolute value of the pressure of the th negative pressure is less than a second threshold value, the air pump is driven to pressurize the second air tank so that the absolute value of the pressure of the th negative pressure reaches the second threshold value.
29. The driving method according to claim 26, wherein when the absolute value of the pressure of the th positive pressure is equal to or greater than a th threshold value and is less than a third threshold value, and the absolute value of the pressure of the th negative pressure is equal to or greater than a second threshold value, the air pump is driven to pressurize the th air tank so that the absolute value of the pressure of the th positive pressure reaches the third threshold value.
30. The driving method as claimed in claim 26, wherein when the absolute value of the pressure of the th positive pressure is greater than or equal to a third threshold value, and the absolute value of the pressure of the th negative pressure is greater than or equal to a second threshold value and less than a fourth threshold value, the air pump is driven to pressurize the second air tank so that the absolute value of the pressure of the th negative pressure reaches the fourth threshold value.
31. The driving method as claimed in claim 26, wherein when the absolute value of the pressure of the positive pressure is greater than or equal to a third threshold value and less than a fifth threshold value, and the absolute value of the pressure of the negative pressure is greater than or equal to a fourth threshold value, the air pump is driven to pressurize the air tank so that the absolute value of the pressure of the positive pressure reaches the fifth threshold value.
32. The method of any of claims 26 to 31 and , wherein the positive pressure creates a second positive pressure within the third reservoir.
33. The driving method of claim 32, wherein said second positive pressure forces sheath fluid within said sheath fluid reservoir into a flow chamber.
34. The method of driving of claim 33 wherein said third air reservoir is disconnected from said th air reservoir before said second positive pressure forces sheath fluid within said sheath fluid reservoir into a flow chamber.
35. The driving method as claimed in claim 34, wherein a pressure change of said second positive pressure is detected by a third pressure sensor when said second positive pressure pushes the sheath fluid in said sheath fluid reservoir into the flow cell.
36. The method of driving of claim 32 wherein the positive pressure creates a third positive pressure within a fourth reservoir.
37. The method as claimed in claim 36, wherein the reservoir is connected to the th reaction cell, and the fourth gas container is connected to the reservoir for providing driving force for the reagent in the reservoir to enter the th reaction cell.
38. The method of claim 36, wherein the negative pressure creates a second negative pressure within a fifth air tank.
39. The driving method as claimed in claim 38, wherein the fifth gas container is conducted to an outlet of the second reaction tank to draw out the liquid in the second reaction tank by the second negative pressure.
40. The driving method according to claim 38, wherein:

    the process of driving the air pump to establish a th positive pressure in the th air tank comprises:

    the air pump establishes a th positive pressure with an absolute value of the pressure greater than a th preset value in the th air storage tank, and conducts the th air storage tank to the atmosphere, so that the absolute value of the pressure of the th positive pressure is reduced to the th preset value;

    and/or the presence of a gas in the gas,

    the process of driving the air pump to establish th negative pressure in the second air tank comprises the following steps:

    the air pump establishes th negative pressure with the absolute pressure value larger than a second preset value in the second air storage tank, and conducts the second air storage tank to the atmosphere, so that the absolute pressure value of the second negative pressure is reduced to the second preset value;

    and/or the presence of a gas in the gas,

    the process at which the positive pressure establishes a second positive pressure within the third tank includes:

    conducting the th air tank and the third air tank to ensure that the th positive pressure is the pressure in the third air tank so as to form a second positive pressure with the absolute value of the pressure larger than a third preset value;

    and/or the presence of a gas in the gas,

    the process of the negative pressure creating a second negative pressure within a fifth tank includes:

    and conducting the fifth gas storage tank to the atmosphere to reduce the absolute value of the second negative pressure to the fourth preset value.
41. The driving method as claimed in any one of claims 25 to 31 and , wherein the quantitative pump of the sample analyzer comprises a liquid chamber and a gas chamber, the liquid chamber connects a liquid storage tank and a reaction tank, the driving method further comprises:

    the liquid storage tank is communicated with the positive pressure so as to push liquid in the liquid storage tank into the liquid chamber by utilizing the positive pressure; and

    the gas chamber is communicated with the positive pressure so as to push the liquid in the liquid chamber to the th reaction tank by using the positive pressure.

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