CN216847390U - Particle analyzer liquid flow system and particle analyzer - Google Patents
Particle analyzer liquid flow system and particle analyzer Download PDFInfo
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- CN216847390U CN216847390U CN202220203920.3U CN202220203920U CN216847390U CN 216847390 U CN216847390 U CN 216847390U CN 202220203920 U CN202220203920 U CN 202220203920U CN 216847390 U CN216847390 U CN 216847390U
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Abstract
The embodiment of the specification discloses particle analyzer liquid flow system and particle analyzer, and particle analyzer liquid flow system includes sheath liquid return circuit, and wherein, sheath liquid return circuit includes: the sheath liquid barrel contains sheath liquid. Sheath liquid injection pump sheath liquid supplies, and the first end and the sheath liquid bucket of sheath liquid injection pump are connected, and the second end and the first filter of sheath liquid injection pump are connected. The first filter filters and removes impurities in the sheath fluid. The first three-way valve is arranged at the first end of the first filter and used for controlling the flow direction of the sheath liquid, wherein one path of the sheath liquid flows to the one-way valve from the first outlet of the first three-way valve. The one-way valve is used to ensure that the sheath fluid flow direction is from the first three-way valve to the flow chamber. The liquid level sensor is located on a first end of the sheath fluid injection pump for detecting a liquid level of the sheath fluid. Therefore, the sheath liquid injection pump with large capacity, high precision and strong stability ensures the stability of sheath liquid supply, and meanwhile, the liquid level sensor automatically detects the capacity of the sheath liquid injection pump to perform dynamic sheath liquid supply, so that the stability of the sheath liquid flow is higher.
Description
Technical Field
The specification relates to the technical field of instruments, in particular to a particle analyzer liquid flow system and a particle analyzer.
Background
The particle analyzer is used for detecting and analyzing a series of important biophysical and biochemical characteristic parameters of various micro particles existing in a sample in a liquid flow system. In the medical field, particle analyzers are widely used in basic research in cell biology, immunology, physiology, molecular biology, etc., and are also used in clinical diagnosis in medicine, etc.
The particle analyzer may include, among other things, a fluid path system. The fluid systems (Fluidics systems) are used for enabling a sample to be detected to form stable sheath flow under the wrapping of sheath fluid, and the sheath flow enables cells in the sample to sequentially flow through a detection area of the flow chamber for detection. According to the principle of hydrodynamic focusing (hydrokinetic focusing), when a sample is ejected from a sample needle and is surrounded into a single cell liquid column under the restriction of sheath liquid, the coaxial flow design enables the flow beam formed by the sample flow and the sheath liquid flow to always keep a layered flow state, and the flow state is called as sheath flow. The sheath Flow is formed in a Flow Cell (Flow Cell), flows through a detection region in the Flow Cell for laser irradiation, and finally flows out from an outlet of the Flow Cell into a waste liquid collection container.
Therefore, the stability of the sheath flow in the flow cell has a significant impact on the stability of the signal detection. Most sheath liquid supply methods of the existing particle analyzers adopt peristaltic pumps, and generated pulses easily disturb sheath flow in a flow chamber, so that the stability of signal detection is influenced.
Disclosure of Invention
An object of the embodiments of the present description is to provide a particle analyzer and a liquid flow system thereof, which only need to supply a sheath liquid in a sample loading process, so that the sheath liquid loss is greatly reduced, the stability of the sheath liquid flow is higher, and the stability of signal detection is further ensured.
In order to solve the above technical problem, the embodiments of the present specification are implemented as follows:
an embodiment of the present specification provides a particle analyzer liquid flow system, which includes a sheath liquid circuit, wherein the sheath liquid circuit includes: the sheath liquid injection pump is connected with the sheath liquid barrel through a first filter;
the sheath liquid barrel is used for containing sheath liquid;
the sheath liquid injection pump is used for supplying sheath liquid, the first end of the sheath liquid injection pump is connected with the sheath liquid barrel, and the second end of the sheath liquid injection pump is connected with the first filter;
the first filter is used for filtering and removing impurities in the sheath liquid;
the first three-way valve is arranged at the first end of the first filter and used for controlling the flow direction of sheath liquid, wherein one path of sheath liquid flows to the one-way valve from a first outlet of the first three-way valve;
the one-way valve is used for ensuring that the flow direction of the sheath fluid is from the first three-way valve to the flow chamber;
the liquid level sensor is located on a first end of the sheath fluid injection pump for detecting a liquid level of the sheath fluid.
Furthermore, the capacity of the sheath liquid injection pump is more than or equal to 300ml, the flow rate of the sheath liquid can quickly and stably reach 20ml/min, and the supply precision of the sheath liquid is more than or equal to 99%.
Further, the sheath fluid circuit includes: a pressure sensor; the pressure sensor is arranged at a first inlet of the flow chamber and used for detecting the pressure of the sheath liquid in real time, so that the sheath liquid enters from the first inlet of the flow chamber and flows to the sample loading loop from a second outlet of the first three-way valve.
Further, the system includes a waste liquid circuit, wherein the waste liquid circuit includes: the device comprises a waste liquid barrel, a waste liquid pump, a waste liquid buffer tank and a one-way valve;
the waste liquid barrel is used for containing waste liquid;
the waste liquid buffer pool is arranged at a liquid path section between the one-way valve and the waste liquid pump, and is used for buffering waste liquid flowing out of the first outlet of the flow chamber and stabilizing the pressure of the first outlet liquid path of the flow chamber;
the waste liquid pump is connected with waste liquid buffer pool and waste liquid bucket for the waste liquid flows in by the first entry in waste liquid buffer pool in waste liquid pump working process, and the zero pressure in the waste liquid buffer pool has been ensured in the unsettled of first export in waste liquid buffer pool, and the waste liquid finally flows in the waste liquid bucket by the second export in waste liquid buffer pool.
Further, the system includes a sample loading loop, wherein the sample loading loop includes: the sample loading pump, the second three-way valve, the sample loading needle and the probe;
the sample loading needle is used for sucking a sheath fluid sample;
the second three-way valve is used for controlling the flow direction of the sheath fluid sample;
when the sheath liquid sample is sucked, the second three-way valve closes the first outlet, and the sheath liquid sample enters a pipeline behind a third outlet of the second three-way valve from the second outlet of the second three-way valve; after the sample is sucked, the first outlet of the second three-way valve is opened, the sheath liquid sample is pushed to the probe by the sample feeding pump, tiny particles in the sheath liquid sample can be queued to form a regular and ordered particle queue after passing through the probe, and the ordered particle queue enters the flow chamber from the second inlet of the flow chamber to be detected.
Further, the system includes a cleaning solution circuit, wherein the cleaning solution circuit includes: the cleaning device comprises a cleaning liquid barrel, a second filter, a third three-way valve, a cleaning pump, a fourth three-way valve and a cleaning block;
under the drive of the cleaning pump, the cleaning liquid flows from the cleaning liquid barrel to the second filter, and the second filter plays a role in filtering impurities in the cleaning liquid;
the third three-way valve is used for controlling the inflow of the cleaning liquid and the sheath liquid, a first outlet of the third three-way valve is a sheath liquid supply inlet, and a second outlet of the third three-way valve is a cleaning liquid supply inlet;
and the fourth three-way valve is used for controlling the cleaning process, a first outlet of the fourth three-way valve controls the cleaning liquid and the sheath liquid to flow in from a third inlet of the flow chamber to perform the cleaning process of the flow chamber, and a second outlet of the fourth three-way valve controls the sheath liquid to flow in from the first inlet of the cleaning block to perform the cleaning process of the outer wall of the sampling needle.
The particle analyzer provided by the embodiments of the present specification includes the particle analyzer fluid flow system described above.
It can be seen from the above technical solutions provided in the embodiments of the present specification that, in the embodiments of the present specification, a sheath fluid injection pump with a large capacity, high precision and strong stability is designed, so that the stability of sheath fluid supply is ensured, and only by starting the sheath fluid injection pump in a sample loading process, the pressure and the flow rate of the sheath fluid in a sheath fluid loop can be quickly and accurately recovered, so that the consumption of the sheath fluid is greatly reduced, and meanwhile, components such as a sheath fluid buffer tank and the like are reduced, so that the structure of the whole fluid path system is simplified, the manufacturing cost is hardly increased, and the maintenance difficulty is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present specification or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments described in the present specification, and for those skilled in the art, other drawings can be obtained according to the drawings without any creative effort.
FIG. 1 is a schematic diagram of a prior art particle analyzer fluid flow system;
fig. 2 is a schematic diagram of a flow system of a particle analyzer according to the present disclosure.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present specification, the technical solutions in the embodiments of the present specification will be clearly and completely described below with reference to the drawings in the embodiments of the present specification, and it is obvious that the described embodiments are only a part of the embodiments of the present specification, and not all of the embodiments. All other embodiments obtained by a person skilled in the art based on the embodiments in the present specification without any inventive step should fall within the scope of protection of the present specification.
As shown in fig. 1, a conventional particle analyzer flow system includes five parts, namely a sheath fluid circuit, a sample loading circuit, a waste fluid circuit, a cleaning fluid circuit and a flow chamber. In FIG. 1, the sheath fluid circuit is shown with a suffix a1 and comprises a sheath fluid barrel M1-a1, a sheath fluid peristaltic pump P1-a1, a filter F1-a1, a three-way valve V1-a1, a one-way valve V2-a1, a buffer pool W1-a1 and a pressure sensor S1-a 1; the sample loading circuit is indicated by a suffix b1 and comprises a sample loading pump P2-b1, a three-way valve V3-b1, a sample loading needle G1-b1 and a probe G2-b 1; the waste liquid loop is shown with the suffix c1 and comprises a waste liquid barrel M2-c1, a waste liquid pump P3-c1 and a one-way valve V4-c 1; the cleaning liquid loop is indicated by the suffix d1 and comprises a cleaning liquid barrel M3-d1, filters F2-d1, three-way valves V5-d1, a cleaning pump P4-d1, three-way valves V6-d1 and cleaning blocks C1-d 1; the flow chamber is indicated at L1.
Referring to FIG. 1, sheath fluid is driven by a sheath fluid peristaltic pump P1-a1 into sheath fluid line a 1. The filter F1-a1 is used for filtering and removing impurities. The three-way valve V1-a1 controls the flow direction of sheath liquid, wherein one path of sheath liquid flows from the outlet 2 of the three-way valve V1-a1 to the one-way valve V2-a1, the one-way valve V2-a1 can ensure that the flow direction of the sheath liquid is from the three-way valve V1-a1 to the buffer pool W1-a1, the risk of sheath liquid backflow cannot be generated, the one-way valve V2-a1 can keep the pressure in the sheath liquid buffer W1-a1, the pressure reduction of the sheath liquid caused in the liquid path maintenance is prevented, the sheath liquid buffer pool W1-a1 plays a role in weakening liquid flow pulse, the pressure sensor S1-a1 is used for detecting the pressure of the sheath liquid in real time, and finally the sheath liquid enters the flow chamber L1 from the inlet L1-1 of the flow chamber; the other sheath flow is from outlet 3 of three-way valve V1-a1 to the sample loading loop.
However, in the section from the sheath liquid peristaltic pump P1-a1 to the flow chamber L1, the physical characteristics of the sheath liquid peristaltic pump P1-a1 are likely to bring about liquid flow pulses, which affect the stability of the liquid flow in the flow chamber L1, and further interfere with signal detection. Although the fluctuation of the liquid flow pulse is weakened by adding the sheath liquid buffer pool W1-a1, in order to ensure the stability of the sheath liquid pressure, the sheath liquid peristaltic pump P1-a1 is required to work continuously, which brings a great deal of sheath liquid waste.
To solve the above technical problem, as shown in fig. 2, a particle analyzer fluid flow system provided by an embodiment of the present disclosure may include a sheath fluid circuit, where the sheath fluid circuit includes: sheath liquid bucket, sheath liquid syringe pump, first filter, first three-way valve and check valve. The sheath liquid barrel is used for containing sheath liquid. The sheath liquid syringe pump is used for sheath liquid to supply, and the first end and the sheath liquid bucket of sheath liquid syringe pump are connected, and the second end and the first filter of sheath liquid syringe pump are connected. The first filter is used for filtering and removing impurities in the sheath liquid. The first three-way valve is arranged at the first end of the first filter and used for controlling the flow direction of sheath liquid, wherein one path of sheath liquid flows to the one-way valve from the first outlet of the first three-way valve. The one-way valve is used to ensure that the sheath fluid flow direction is from the first three-way valve to the flow chamber.
The flow chamber is used for coating a sample to be detected with sheath fluid, ensuring the places where the micro particles pass in a queue mode, and performing signal acquisition and analysis in the flow chamber by means of light detection and the like, wherein the stability of the sheath fluid flow in the flow chamber has an important influence on the stability of signal detection.
Illustratively, in FIG. 2, the sheath fluid circuit is indicated by a suffix a2 and comprises a sheath fluid barrel M1-a2, a sheath fluid injection pump P1-a2, a first filter F1-a2, a first three-way valve V1-a2, a one-way valve V2-a2, a pressure sensor S1-a2 and a liquid level sensor T1-a 2; the flow chamber is indicated at L2.
Wherein the sheath fluid infusion pump shown in fig. 2 is more stable, has an increased accuracy of at least one order of magnitude, and is capable of maintaining a constant delivery rate, relative to the peristaltic pump of fig. 1. Among them, the sheath liquid injection pump P1-a2 has a large capacity and high accuracy. Furthermore, the capacity of the sheath liquid injection pump is more than or equal to 300ml, the flow rate of the sheath liquid can rapidly and stably reach 20ml/min, and the supply precision of the sheath liquid is more than or equal to 99%. Therefore, in the embodiment of the specification, the sheath liquid buffer pool W1-a1 is reduced, so that the liquid path structure is simplified, and the maintenance is simpler. The physical characteristics of the sheath liquid injection pump P1-a2 ensure more accurate and stable sheath liquid supply, almost no liquid flow pulse caused by a peristaltic pump exists, the requirement of fast and stable sheath liquid supply can be met only by starting the sheath liquid injection pump P1-a2 in the sample loading process, and the consumption of the sheath liquid is greatly reduced.
It can be seen that, the sheath liquid injection pump with large capacity, high precision and strong stability is designed in the embodiment of the application, the stability of sheath liquid supply is ensured, the pressure of a sheath liquid loop and the flow rate of the sheath liquid can be quickly and accurately recovered only by starting the sheath liquid injection pump in the sample loading process, the consumption of the sheath liquid is greatly reduced, components such as a sheath liquid buffer pool are reduced, the structure of the whole liquid path system is simplified, the manufacturing cost is hardly increased, and the maintenance difficulty is reduced.
Further, the sheath fluid circuit may include: a liquid level sensor; the liquid level sensor is located on a first end of the sheath fluid injection pump for detecting a liquid level of the sheath fluid. As shown in fig. 2, the sheath fluid injection pump P1-a2 has the characteristics of high precision and stability, but needs continuous liquid supply, and in order to overcome the problem, a liquid level sensor T1-a2 is added, and the volume of the sheath fluid injection pump P1-a2 is automatically detected by software, so that the sheath fluid is dynamically supplied, and the stable operation of the fluid flow system is ensured.
Further, the sheath fluid circuit may include: a pressure sensor. As shown in FIG. 2, pressure sensor S1-a 2. The pressure sensor S1-a2 is arranged at the first inlet L1-1 of the flow chamber L2 and is used for detecting the pressure of the sheath liquid in real time, so that the sheath liquid enters from the first inlet L1-1 of the flow chamber L2 and flows to the sample loading loop from the second outlet 3 of the first three-way valve V1-a 2.
Further, the system may include a waste liquid circuit, wherein the waste liquid circuit may include: the waste liquid barrel, the waste liquid pump, the waste liquid buffer pool and the one-way valve. The waste liquid bucket is used for holding the waste liquid. The waste liquid buffer pool is arranged at a liquid path section between the one-way valve and the waste liquid pump, and is used for buffering waste liquid flowing out from a first outlet of the flow chamber and stabilizing the pressure of the first outlet liquid path of the flow chamber. The waste liquid pump is connected with waste liquid buffer pool and waste liquid bucket for the waste liquid flows in by the first entry in waste liquid buffer pool in waste liquid pump working process, and the zero pressure in the waste liquid buffer pool has been ensured in the unsettled of first export in waste liquid buffer pool, and the waste liquid finally flows in the waste liquid bucket by the second export in waste liquid buffer pool.
Illustratively, in FIG. 2, the waste circuit is designated with the suffix c2 and includes a waste barrel M2-c2, a waste liquid pump P3-c2, a waste liquid buffer tank W1-c2, and a one-way valve V4-c 2. And a waste liquid buffer pool W1-c2 is added to a liquid path between the one-way valve V4-c2 and the waste liquid pump P3-c 2. The waste liquid buffer pool W1-c2 is used for buffering waste liquid flowing from the first outlet L2-3 of the flow chamber L2, plays a role in stabilizing the pressure of the liquid path at the opening L2-3 of the flow chamber, stabilizes sheath liquid flow in the flow chamber L2 and improves the stability of signal detection. In the working process of the waste liquid pump P3-c2, waste liquid flows in from an inlet 2 of the waste liquid buffer pool W1-c2, an outlet 3 is suspended to ensure zero pressure in the waste liquid buffer pool W1-c2, and the waste liquid finally flows into a waste liquid barrel from an outlet 1 of the waste liquid buffer pool, and the pressure difference from a c2 loop to the inside of the flow chamber L2 cannot be influenced by the height of the placement position of the waste liquid barrel M2-c1 because positive pressure does not exist in the waste liquid buffer pool W1-c 2.
Therefore, the embodiment of the application designs a waste liquid buffer pool, so that the pressure difference of the waste liquid loop is further stabilized, the stability of the sheath liquid flow in the flow chamber is improved, the signal detection is not interfered, the method is very convenient, and the use efficiency is not influenced.
Further, the system may include a sample loading circuit, wherein the sample loading circuit comprises: sample loading pump, second three-way valve, sample loading needle and probe. The loading needle is used for sucking sheath fluid samples. The second three-way valve is used for controlling the flow direction of the sheath fluid sample. When the sheath liquid sample is sucked, the second three-way valve closes the first outlet, and the sheath liquid sample enters a pipeline behind a third outlet of the second three-way valve from the second outlet of the second three-way valve; after the sample is sucked, the first outlet of the second three-way valve is opened, the sheath liquid sample is pushed to the probe by the sample feeding pump, tiny particles in the sheath liquid sample can be queued to form a regular and ordered particle queue after passing through the probe, and the ordered particle queue enters the flow chamber from the second inlet of the flow chamber to be detected.
Illustratively, in FIG. 2, the loading circuit is shown with the suffix b2 and includes a loading pump P2-b2, a second three-way valve V3-b2, a loading needle G1-b2, and a probe G2-b 2. The sample is sucked by a sample feeding needle G1-b2, a second three-way valve V3-b2 is used for controlling the flow direction of the sample, when the sample is sucked, an outlet 2 is closed, and the sample enters a pipeline behind an outlet 1 from an outlet 3; after the sample is sucked, the outlet 2 is opened, the sample is pushed to the probes G2-b2 by the sample loading pump P2-b2, the tiny particles in the sample are queued after passing through the probes G2-b2 to form regular and ordered particle queues, and finally the ordered tiny particle queues enter the flow chamber L2 from the inlet L2-2 of the flow chamber for sample detection.
Further, the system may include a cleaning solution circuit, wherein the cleaning solution circuit includes: the cleaning device comprises a cleaning liquid barrel, a second filter, a third three-way valve, a cleaning pump, a fourth three-way valve and a cleaning block. Under the drive of the cleaning pump, the cleaning liquid flows to the second filter from the cleaning liquid barrel, and the second filter plays a role in filtering impurities in the cleaning liquid. The third three-way valve is used for controlling the inflow of the cleaning liquid and the sheath liquid, a first outlet of the third three-way valve is a sheath liquid supply inlet, and a second outlet of the third three-way valve is a cleaning liquid supply inlet. And the fourth three-way valve is used for controlling the cleaning process, a first outlet of the fourth three-way valve controls the cleaning liquid and the sheath liquid to flow in from a third inlet of the flow chamber to perform the cleaning process of the flow chamber, and a second outlet of the fourth three-way valve controls the sheath liquid to flow in from the first inlet of the cleaning block to perform the cleaning process of the outer wall of the sampling needle.
Illustratively, in FIG. 2, the cleaning solution circuit is shown with the suffix d2 and includes a cleaning solution tank M3-d2, a second filter F1-d2, a third three-way valve V5-d2, a cleaning pump P4-d2, a fourth three-way valve V6-d2, and a cleaning block C1-d 2. The cleaning liquid is driven by the cleaning pump P4-d2 to flow from the cleaning liquid barrel M3-d2 to the second filter F1-d2, and the second filter F1-d2 plays a role in filtering impurities in the cleaning liquid. The third three-way valve V5-d2 is used to control the inflow of the cleaning liquid and the sheath liquid, and the outlet 2 is the sheath liquid supply inlet and the outlet 3 is the cleaning liquid supply inlet. The fourth three-way valve V6-d2 is used for controlling a cleaning flow, the outlet 2 controls the cleaning liquid and the sheath liquid to flow into the flow chamber L2 from the flow chamber inlet L2-4 in sequence to perform the cleaning flow of the flow chamber L2, and the outlet 3 controls the sheath liquid to flow into the cleaning block from the first inlet 1 to perform the cleaning flow of the outer wall of the sampling needle G1-b 2.
A particle analyzer provided in an embodiment of this specification includes the above-mentioned particle analyzer liquid flow system, which is described in detail in the above-mentioned embodiment and is not described herein again.
The embodiments in the present specification are described in a progressive manner, and the same and similar parts among the embodiments are referred to each other, and each embodiment focuses on the differences from the other embodiments. In particular, for the system embodiment, since it is substantially similar to the method embodiment, the description is simple, and for the relevant points, reference may be made to the partial description of the method embodiment.
The above description is only an example of the present specification, and is not intended to limit the present specification. Various modifications and alterations to this description will become apparent to those skilled in the art. Any modification, equivalent replacement, improvement or the like made within the spirit and principle of the present specification should be included in the scope of the claims of the present specification.
Claims (7)
1. A particle analyzer fluid flow system, comprising a sheath fluid circuit, wherein the sheath fluid circuit comprises: the sheath liquid injection pump is connected with the sheath liquid barrel through a first filter;
the sheath liquid barrel is used for containing sheath liquid;
the sheath liquid injection pump is used for supplying sheath liquid, a first end of the sheath liquid injection pump is connected with the sheath liquid barrel, and a second end of the sheath liquid injection pump is connected with the first filter;
the first filter is used for filtering and removing impurities in the sheath fluid;
the first three-way valve is arranged at the first end of the first filter and used for controlling the flow direction of sheath liquid, wherein one-way sheath liquid flows to the one-way valve from a first outlet of the first three-way valve;
the one-way valve is used for ensuring that the sheath fluid flow direction is from the first three-way valve to a flow chamber;
the liquid level sensor is located on a first end of the sheath liquid injection pump and is used for detecting the liquid level of the sheath liquid.
2. The system of claim 1, wherein the sheath fluid injection pump has a capacity of 300ml or more, a sheath fluid flow rate of 20ml/min that can be rapidly stabilized, and a sheath fluid supply accuracy of 99% or more.
3. The system of claim 1 or 2, wherein the sheath fluid circuit comprises: a pressure sensor; the pressure sensor is arranged at a first inlet of the flow chamber and used for detecting the pressure of the sheath liquid in real time, so that the sheath liquid enters from the first inlet of the flow chamber and flows to the sample loading loop from a second outlet of the first three-way valve.
4. The system of claim 1, comprising a waste liquid circuit, wherein the waste liquid circuit comprises: the device comprises a waste liquid barrel, a waste liquid pump, a waste liquid buffer tank and a one-way valve;
the waste liquid barrel is used for containing waste liquid;
the waste liquid buffer pool is arranged at a liquid path section between the one-way valve and the waste liquid pump, and is used for buffering waste liquid flowing out of the first outlet of the flow chamber and stabilizing the pressure of the first outlet liquid path of the flow chamber;
the waste liquid pump is connected with the waste liquid buffer pool and the waste liquid barrel and used for enabling waste liquid to flow in from a first inlet of the waste liquid buffer pool in the working process of the waste liquid pump, a first outlet of the waste liquid buffer pool is suspended to ensure zero pressure in the waste liquid buffer pool, and the waste liquid finally flows into the waste liquid barrel from a second outlet of the waste liquid buffer pool.
5. The system of claim 1, comprising a sample loading loop, wherein the sample loading loop comprises: the sample loading pump, the second three-way valve, the sample loading needle and the probe;
the sample loading needle is used for sucking a sheath fluid sample;
the second three-way valve is used for controlling the flow direction of the sheath fluid sample;
when the sheath fluid sample is sucked, the second three-way valve closes the first outlet, and the sheath fluid sample enters a pipeline behind the third outlet of the second three-way valve from the second outlet of the second three-way valve; after the sample is sucked, the first outlet of the second three-way valve is opened, the sheath liquid sample is pushed to the probe by the sample feeding pump, the tiny particles in the sheath liquid sample can be queued to form a regular and ordered particle queue after passing through the probe, and the ordered particle queue enters the flow chamber from the second inlet of the flow chamber to be detected by the sheath liquid sample.
6. The system of claim 1, comprising a cleaning solution circuit, wherein the cleaning solution circuit comprises: the cleaning device comprises a cleaning liquid barrel, a second filter, a third three-way valve, a cleaning pump, a fourth three-way valve and a cleaning block;
under the driving of the cleaning pump, cleaning liquid flows from the cleaning liquid barrel to the second filter, and the second filter plays a role in filtering impurities in the cleaning liquid;
the third three-way valve is used for controlling the inflow of the cleaning liquid and the sheath liquid, a first outlet of the third three-way valve is a sheath liquid supply inlet, and a second outlet of the third three-way valve is the cleaning liquid supply inlet;
the fourth three-way valve is used for controlling a cleaning process, a first outlet of the fourth three-way valve controls the cleaning liquid and the sheath liquid to flow in from a third inlet of the flow chamber to perform the cleaning process of the flow chamber, and a second outlet of the fourth three-way valve controls the sheath liquid to flow in from the first inlet of the cleaning block to perform the cleaning process of the outer wall of the sampling needle.
7. A particle analyzer including a particle analyzer fluid system according to any one of claims 1 to 6.
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| CN202220203920.3U CN216847390U (en) | 2022-01-25 | 2022-01-25 | Particle analyzer liquid flow system and particle analyzer |
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