CN101451989B - Flow-type cell instrument and waste liquid discharge device and method thereof - Google Patents
Flow-type cell instrument and waste liquid discharge device and method thereof Download PDFInfo
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- CN101451989B CN101451989B CN 200710077561 CN200710077561A CN101451989B CN 101451989 B CN101451989 B CN 101451989B CN 200710077561 CN200710077561 CN 200710077561 CN 200710077561 A CN200710077561 A CN 200710077561A CN 101451989 B CN101451989 B CN 101451989B
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Abstract
The invention discloses a flow cytometry and a waste liquid eductor thereof and a method. The waste liquid eductor of the flow cytometry comprises a waste liquid discharge pipe, a first branch pipe, a second branch pipe and a pressure fluctuation isolated unit; the first branch pipe is connected with an inlet end of the waste liquid discharge pipe and an outlet of a streamer chamber; the second branch pipe is connected with a waste liquid pump of the flow cytometry and the inlet end of the waste liquid discharge pipe; the pressure fluctuation isolated unit is connected in the inlet end of the waste liquid discharge pipe and the pipeline of the streamer chamber in series. Use of the flow cytometry in the inventive technical scheme, due to the pressure fluctuation isolated unit, effectively isolates unstable fluid caused by pressure fluctuation generated by the waste liquid pump, therefore, no adverse effect is caused on the sample detection in the streamer chamber when the waste liquid pump works.
Description
Technical field
The present invention relates to cell detection and analysis technical field, be specifically related to a kind of waste liquid discharge device of flow cytometer and comprise the flow cytometer of this waste liquid discharge device, and a kind of discharging of waste liquid method of flow cytometer.
Background technology
Detect and the particle analysis field at blood cell, often make the queuing of cell to be detected or particle flux by an aperture, detect by method optics or electricity at the aperture place, analyze the quantity, volume information and the internal feature that obtain cell or particle.In order to make cell or particle more regular by the path of aperture, can introduce a kind of clean liquid (sheath fluid) and be wrapped in around the sample to be detected, thereby guarantee that cell or particle are in the centre position of aperture when lining up by aperture, thus convenient the detection.Its principle as shown in Figure 1, inject the sample particle of flow chamber 6 by specimen needle 7, under the parcel of the sheath fluid that injects flow chamber 6 from sheath fluid entrance 8, queuing is by the aperture of flow chamber 6, thereby convenient the detection, this method is called as flow cytometry (Flow cytometry), realizes that the device of flow cytometry abbreviates flow cytometer as.
General flow cytometer as shown in Figure 2, comprise sample pool 1, negative pressure pond 2, sample syringe 3, sheath liquid pool 4, waste liquid pool 5, flow chamber 6 and waste drains pump 9, wherein flow chamber 6 has the sheath fluid entrance 8 that flows into for sheath fluid and the specimen needle of injecting for sample to be tested 7.Sample pool 1 is used for the good sample of storage reaction.Negative pressure pond 2 is communicated with extraneous negative pressure source, make resident negative pressure in it, be used for carrying out sample and prepare, described sample is prepared to be about to sample in the sample pool 1 and is drawn in the pipeline between sample syringe 3 and the flow chamber 6, and the sample that is in this pipeline is sample to be detected.Sample syringe 3 is used for driving sample to be tested and enters flow chamber 6 formation sample flow by specimen needle 7.Sheath liquid pool 4 is used for storing sheath fluid, and it makes resident malleation in it by being connected with extraneous positive pressure source, and utilizes this malleation to drive sheath fluid to enter flow chamber 6 through sheath fluid entrance 8 and form sheaths and flow.Waste liquid pool 5 is used for the storage waste liquid.Waste drains pump 9 is used to the discharging of waste liquid in the sample pool 1 that driving force is provided.Sample pool 1 is connected by the main interface of pipeline with sample syringe 3, and the main interface of sample syringe 3 also is connected with specimen needle 7 and negative pressure pond 2 respectively.Sheath liquid pool 4 is connected with sheath fluid entrance 8.Flow chamber 6 also is connected by first arm that is connected with its outlet and threeway 12, by the discharging of waste liquid pipe between threeway 12 and the waste liquid pool 5, waste liquid is discharged into waste liquid pool 5 then.Sample pool 1 connects waste drains pump 9 by pipeline, and waste drains pump 9 is communicated with by second arm and threeway 12.Waste liquid in the sample pool 1 is through waste drains pump 9 and second arm, converges at threeway 12 place and flow chamber 6 waste liquid through first tube drainage, is discharged into waste liquid pool 5 through the discharging of waste liquid pipe together then.
If yet this flow cytometer in flow chamber 6, carry out using waste drains pump 9 in the cell detection, will cause the pressure surge in flow chamber 6 exits, thereby influence the stability of sample flow in the flow chamber 6, and then influence cell detection.There are the problems referred to above in the flow cytometer that not only drives for above-mentioned constant pressure source in fact, and also has the problems referred to above for constant pressure source being driven the flow cytometer that changes the driving of constant current amount into.In fact as long as waste drains pump 9 shares a discharging of waste liquid pipe with flow chamber 6, make the pressure in flow chamber 6 exits produce fluctuation when just inevitably existing above-mentioned waste drains pump 9 to work, and then influence the problem of cell detection.
Summary of the invention
The objective of the invention is to propose a kind of new flow cytometer and the method for eliminating the flow disturbance of flow cytometer, waste drains pump influences the stable problem of flow chamber sheath stream in the above-mentioned prior art to overcome.
To achieve the above object of the invention, the technical solution used in the present invention is:
A kind of waste liquid discharge device, it is used for the discharging of flow cytometer waste liquid, comprise the discharging of waste liquid pipe, and first arm and second arm that are communicated with the inlet end of described discharging of waste liquid pipe, described first arm is used for linking to each other with the outlet of flow cytometer flow chamber, described second arm is used for linking to each other with the waste drains pump of flow cytometer, also comprises the isolated from pressure fluctuations unit; Described isolated from pressure fluctuations unit strings is connected in the pipeline of first arm, perhaps is serially connected between discharging of waste liquid tube inlet end and first arm.
In the optimized technical scheme, described isolated from pressure fluctuations unit comprises volume container; Described volume container is serially connected in the pipeline of first arm.
Further, described isolated from pressure fluctuations unit also comprises current limiting tube, and described current limiting tube and volume container polyphone.
In the optimized technical scheme, described isolated from pressure fluctuations unit comprises volume container; Described volume container is serially connected between the inlet end and first arm of discharging of waste liquid pipe, and second arm is communicated with by the inlet end of volume container with the discharging of waste liquid pipe.
Further, described isolated from pressure fluctuations unit also comprises current limiting tube, and described current limiting tube is serially connected in the pipeline of first arm, perhaps is serially connected between discharging of waste liquid tube inlet end and first arm.
A kind of flow cytometer comprises: waste liquid discharge device, flow chamber and waste drains pump; Described waste liquid discharge device comprises the discharging of waste liquid pipe, and first arm and second arm that are communicated with the inlet end of described discharging of waste liquid pipe, described first arm links to each other with the outlet of described flow chamber, and described second arm is connected with waste drains pump, also comprises the isolated from pressure fluctuations unit; Described isolated from pressure fluctuations unit strings is connected between discharging of waste liquid tube inlet end and the flow chamber.
In the optimized technical scheme, described isolated from pressure fluctuations unit comprises volume container.
Further, described isolated from pressure fluctuations unit also comprises current limiting tube, and described current limiting tube is connected with described volume container.
A kind of discharging of waste liquid method of flow cytometer, it is by arranging the waste liquid that waste liquid discharge device discharges flow cytometer, waste liquid discharge device comprises the discharging of waste liquid pipe, and it comes the waste liquid of discharge flow chamber by be connected the outlet of flow chamber with first arm that discharging of waste liquid tube inlet end is communicated with; Be connected waste drains pump by second arm with the connection of discharging of waste liquid tube inlet end, it is also by in the pipeline between discharging of waste liquid tube inlet end and flow chamber, serial connection isolated from pressure fluctuations unit comes the isolated pressure fluctuation, to eliminate the influence that pressure surge is flowed to the indoor liquid that flows.
In the optimized technical scheme, it is by being connected in series volume container in the pipeline between discharging of waste liquid tube inlet end and flow chamber, be connected in series current limiting tube again after perhaps being connected in series volume container, come the isolated pressure fluctuation, to eliminate the influence that pressure surge is flowed to the indoor liquid that flows.
After adopting technique scheme, the present invention can reach following remarkable result:
Owing to be provided with the isolated from pressure fluctuations unit, the pressure surge that produces in the time of can effectively completely cut off waste drains pump work causes fluid problem of unstable in the flow chamber, thereby waste drains pump can not cause harmful effect to the pattern detection in the flow chamber when working again.
Because the isolated from pressure fluctuations unit is the volume container with a constant volume, and is simple and practical, effective.
Owing to further be provided with the current limiting tube of contacting with volume container, can under the less situation of the volume of volume container, reach filter effect preferably.
Because the end of second arm is communicated with volume container, has omitted the threeway head, and can play the effect of isolated pressure surge preferably.
Owing between volume container and flow chamber, further be provided with current limiting tube, can under the less situation of the volume of volume container, reach filter effect preferably.
Description of drawings
Fig. 1 is the principle schematic of flow cytometry;
Fig. 2 is the liquid line structure figure of general flow cytometer;
Fig. 3 is the liquid line structure figure of the specific embodiment of the invention one streaming cell instrument;
Fig. 4 is the liquid line structure figure of the specific embodiment of the invention two streaming cell instruments;
Fig. 5 is the liquid line structure figure of the specific embodiment of the invention three flow cytometers.
Embodiment
Embodiment one
The flow cytometer that this embodiment provides comprises sample pool 1, negative pressure pond 2, sample syringe 3, sheath liquid pool 4, waste liquid pool 5, flow chamber 6 and waste drains pump 9 as shown in Figure 3.Wherein flow chamber 6 has the sheath fluid entrance 8 that flows into for sheath fluid, for the specimen needle 7 of sample to be tested injection, and the outlet that supplies waste liquid to discharge.Sample pool 1 is used for the good sample of storage reaction.Negative pressure pond 2 is communicated with extraneous negative pressure source, makes resident negative pressure in it, is used for carrying out sample and prepares.Sample prepare to be exactly the sample in the sample pool 1 is drawn in the pipeline between sample syringe 3 and the flow chamber 6, and the sample that is in this pipeline is sample to be detected.Sample syringe 3 is used for driving sample to be tested and enters flow chamber 6 formation sample flow by specimen needle 7.Sheath liquid pool 4 is used for storing sheath fluid, and it makes resident malleation in it by being connected with extraneous positive pressure source, and utilizes this malleation to drive sheath fluid to enter flow chamber 6 through sheath fluid entrance 8 and form sheaths and flow.Waste liquid pool 5 is used for the storage waste liquid.Waste drains pump 9 is used to the discharging of waste liquid in the sample pool 1 that driving force is provided.Sample pool 1 is connected by the main interface of pipeline with sample syringe 3, and the main interface of sample syringe 3 also is connected with specimen needle 7 and negative pressure pond 2 respectively.Sheath liquid pool 4 is connected with sheath fluid entrance 8.Flow chamber 6 also is connected by first arm that is connected with its outlet and threeway 12, via the discharging of waste liquid pipe between threeway 12 and the waste liquid pool 5, waste liquid is discharged into waste liquid pool 5 then.The outlet of sample pool 1 connects waste drains pump 9 by pipeline, and waste drains pump 9 connects threeway 12 by second arm.Waste liquid in the sample pool 1 through second arm, enters the discharging of waste liquid pipe between threeway 12 and the waste liquid pool 5 under the driving of waste drains pump 9, be discharged into waste liquid pool 5 with the waste liquid of flow chamber 6.
The special feature of this flow cytometer is, the pipeline of first arm between threeway 12 and flow chamber 6 is provided with the isolated from pressure fluctuations unit, cut off between hydrodynamic pressure fluctuation in the pipeline that waste drains pump 9 is caused and the flow chamber 6, when eliminating waste drains pump 9 work with this to the influence of flow chamber 6 interior liquid flows.Particularly, be at the pipeline of first arm volume container 10 of having connected.Volume container 10 has bigger volume, and it can effectively cut off the pressure surge of being transmitted by the liquid in the pipeline by waste drains pump 9.For the volume that makes volume container 10 can be a little bit smaller, to reduce its volume, also at volume container 10 current limiting tube 11 of having connected with the pipeline between the flow chamber 6, make the isolated from pressure fluctuations unit under the situation that the volume of volume container 10 reduces, also isolated pressure fluctuation preferably.
Embodiment two
The flow cytometer that this embodiment provides as shown in Figure 4, comprises sample pool 1, sample syringe 3, sheath liquid pool 4, waste liquid pool 5, flow chamber 6, waste drains pump 9 and constant current amount drive unit.Wherein flow chamber 6 has the sheath fluid entrance 8 that flows into for sheath fluid, for the specimen needle 7 of sample to be tested injection, and the outlet that supplies waste liquid to discharge.Sample pool 1 is used for the good sample of storage reaction.Sheath liquid pool 4 is used for storing sheath fluid.Waste liquid pool 5 is used for the storage waste liquid.Waste drains pump 9 is used to the discharging of waste liquid in the sample pool 1 that driving force is provided.In this embodiment, constant current amount drive unit is sheath stream syringe 21, and it is used for carrying out sample and prepares and draw sheath fluid in the sheath liquid pool 4, and provides driving that sheath fluid is injected into by sheath fluid entrance 8 to form sheath stream in the flow chamber 6.It is exactly that sample to be detected is drawn onto in the pipeline between specimen needle 7 and the sample syringe 3 that described sample is prepared, and the sample that is in this pipeline is sample to be detected.Sample syringe 3 is used for driving sample to be tested and enters flow chamber 6 formation sample flow by specimen needle 7.
Its course of work is as follows: at first be communicated with the pipeline between sheath stream syringe 21 and the sample pool 1, sheath stream syringe 21 resorptions are drawn into sample in the pipeline between sample syringe 3 and the specimen needle 7.Be communicated with the pipeline between sheath stream syringe 21 and the sheath liquid pool 4 then, sheath stream syringe 21 continues resorptions, and sheath fluid is drawn among the sheath stream syringe 21.Be communicated with the pipeline between sheath stream syringe 21 and the sheath fluid entrance 8 then, sheath stream syringe 21 injects the stable sheath stream of flow chamber 6 formation with sheath fluid wherein.After treating that stable sheath stream forms, promote sample syringe 3, the sample of preparing before this in pipeline is injected the stable sample flow of flow chamber 6 formation from specimen needle 7, can begin then to detect.The waste liquid of flow chamber 6 is discharged into waste liquid pool 5 by first arm, threeway 12 and discharging of waste liquid pipe.Waste liquid behind the cleaning sample pool 1, behind waste drains pump 9 and second arm, the waste liquid in threeway 12 place and first arm is joined, and is discharged into waste liquid pool 5 through the discharging of waste liquid pipe together then.
The flow cytometer of this embodiment produces pressure surge when also having waste drains pump 9 work, influences the stable problem of flow chamber 6 inner fluids.So also the pipeline of first arm between threeway 12 and flow chamber 6 arranges the isolated from pressure fluctuations unit, cut off between hydrodynamic pressure fluctuation in the pipeline that waste drains pump 9 is caused and the flow chamber 6, when eliminating waste drains pump 9 work with this, to the influence of flow chamber 6 inner fluids.Particularly, be that pipeline at first arm is provided with volume container 10.Volume container 10 has bigger volume, and it can effectively cut off the pressure surge of being transmitted by the liquid in the pipeline by waste drains pump 9.For the volume that makes volume container 10 can be a little bit smaller, to reduce its volume, the current limiting tube 11 of having connected of the pipeline between volume container 10 and threeway 12 also, under the less situation of the volume of volume container 10, also isolated pressure fluctuation preferably.
This embodiment and embodiment once difference be, used the sheath stream syringe 21 of constant current amount to drive sheath fluid and flowed.In addition, current limiting tube is serially connected between volume container 10 and the threeway 12, rather than between volume container 10 and the flow chamber 6.
Embodiment three
The flow cytometer of this embodiment as shown in Figure 5, be to have cancelled threeway 12 with the difference of embodiment two, and volume container 10 itself has two entrances and an outlet.The waste liquid that flow chamber 6 is discharged through first arm enters volume container 10 by an entrance; The waste liquid of coming through second arm from waste drains pump 9 enters volume container 10 by another entrance, and the outlet by volume container 10 together is discharged into waste liquid pool 5 through the discharging of waste liquid pipe then.Simultaneously, for the volume that makes volume container 10 a little bit smaller, to reduce its volume, the current limiting tube 11 of having connected in the pipeline between volume container 10 and flow chamber 6, thereby under the less situation of the volume of volume container 10, also isolated pressure fluctuation preferably.
Above content is to further describing that the present invention does in conjunction with concrete preferred implementation, but clearly, more than description and content illustrated in the accompanying drawings all should be understood to be exemplary, can not assert that concrete enforcement of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, these all should be considered as belonging to protection scope of the present invention.
Claims (8)
1. waste liquid discharge device, it is used for the discharging of flow cytometer waste liquid, comprise the discharging of waste liquid pipe, and first arm and second arm that are communicated with the inlet end of described discharging of waste liquid pipe, described first arm is used for linking to each other with the outlet of flow cytometer flow chamber, and described second arm is used for linking to each other with the waste drains pump of flow cytometer, it is characterized in that, also comprise the isolated from pressure fluctuations unit, described isolated from pressure fluctuations unit comprises volume container; Described volume container is serially connected in the pipeline of first arm, perhaps between the described volume container inlet end that is serially connected in the discharging of waste liquid pipe and first arm and second arm be communicated with by the inlet end of volume container with the discharging of waste liquid pipe.
2. waste liquid discharge device according to claim 1 is characterized in that, described volume container is serially connected in the pipeline of first arm.
3. waste liquid discharge device according to claim 2 is characterized in that, described isolated from pressure fluctuations unit also comprises current limiting tube, and described current limiting tube and volume container polyphone.
4. waste liquid discharge device according to claim 1 is characterized in that, described isolated from pressure fluctuations unit also comprises current limiting tube, and described current limiting tube is serially connected in the pipeline of first arm, perhaps is serially connected between discharging of waste liquid tube inlet end and first arm.
5. a flow cytometer comprises: waste liquid discharge device, flow chamber and waste drains pump; Described waste liquid discharge device comprises the discharging of waste liquid pipe, described discharging of waste liquid pipe comprises first arm and second arm that its inlet end is told, described first arm links to each other with the outlet of described flow chamber, described second arm is connected with waste drains pump, it is characterized in that, also comprise the isolated from pressure fluctuations unit, described isolated from pressure fluctuations unit comprises volume container; Described volume container is serially connected in the pipeline of first arm, perhaps between the described volume container inlet end that is serially connected in the discharging of waste liquid pipe and first arm and second arm be communicated with by the inlet end of volume container with the discharging of waste liquid pipe.
6. flow cytometer according to claim 5 is characterized in that, described isolated from pressure fluctuations unit also comprises current limiting tube, and described current limiting tube is connected with described volume container.
7. the discharging of waste liquid method of a flow cytometer, it is by arranging the waste liquid that waste liquid discharge device discharges flow cytometer, waste liquid discharge device comprises the discharging of waste liquid pipe, and its first arm of telling by discharging of waste liquid tube inlet end connects the outlet of flow chamber, comes the waste liquid of discharge flow chamber; Second arm of telling by discharging of waste liquid tube inlet end connects waste drains pump, it is characterized in that, it also comes the isolated pressure fluctuation by serial connection isolated from pressure fluctuations unit in first arm, and described isolated from pressure fluctuations unit comprises volume container.
8. the discharging of waste liquid method of flow cytometer according to claim 7 is characterized in that, it comes the isolated pressure fluctuation by the current limiting tube of contacting again behind the volume container of serial connection in the pipeline of first arm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200710077561 CN101451989B (en) | 2007-11-29 | 2007-11-29 | Flow-type cell instrument and waste liquid discharge device and method thereof |
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| CN 200710077561 CN101451989B (en) | 2007-11-29 | 2007-11-29 | Flow-type cell instrument and waste liquid discharge device and method thereof |
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| CN101451989A CN101451989A (en) | 2009-06-10 |
| CN101451989B true CN101451989B (en) | 2013-08-28 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102564919B (en) * | 2010-12-31 | 2014-03-19 | 深圳迈瑞生物医疗电子股份有限公司 | Flow chamber component, flow chamber blockage removing method and sample analyzer |
| CN102854095A (en) * | 2011-06-27 | 2013-01-02 | 深圳理邦实验生物电子有限公司 | Detection device and method for stream mode particle |
| CN104749085B (en) * | 2013-12-31 | 2019-09-03 | 深圳迈瑞生物医疗电子股份有限公司 | Particle analyzer liquid fluid system and particle analyzer |
| CN105675897A (en) * | 2016-04-13 | 2016-06-15 | 深圳市锦瑞生物科技有限公司 | Cell analyzer |
| CN108627449B (en) * | 2017-03-23 | 2023-03-31 | 深圳市帝迈生物技术有限公司 | Flow cytometer fluid system and measuring method |
| WO2019028940A1 (en) * | 2017-08-09 | 2019-02-14 | 利多(香港)有限公司 | Sheath flow impedance counting device and flow cytometry analyzer comprising device |
| CN111033254A (en) | 2017-08-31 | 2020-04-17 | 深圳迈瑞生物医疗电子股份有限公司 | Sample detection device, sample analyzer and sample detection method |
| CN111587371B (en) * | 2017-12-30 | 2024-06-07 | 深圳迈瑞生物医疗电子股份有限公司 | Particle analyzer adopting sheath flow impedance method and measuring method thereof |
| CN113125330B (en) * | 2021-03-29 | 2023-09-19 | 深圳市科曼医疗设备有限公司 | Flow cytometry analyzer |
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| WO2006060770A2 (en) * | 2004-12-03 | 2006-06-08 | Xy, Inc. | Pressure regulated continuously variable volume container for fluid delivery |
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| US5934885A (en) * | 1994-10-07 | 1999-08-10 | Bayer Corporation | Reagent pump assembly |
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| WO2007106376A2 (en) * | 2006-03-10 | 2007-09-20 | Mcu Designs, Inc. | High speed sample supply device |
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Effective date of registration: 20180621 Address after: 518057 the 1-4 floor of MINDRAY building, science and technology south twelve Road, Nanshan District high tech Industrial Park, Shenzhen, Guangdong. Co-patentee after: Shenzhen MINDRAY Technology Co., Ltd. Patentee after: Shenzhen Mairui Biotherapeutic Electronic Co., Ltd. Address before: 518057 MINDRAY science and technology south twelve road MINDRAY high tech Industrial Park, Shenzhen, Guangdong Patentee before: Shenzhen Mairui Biotherapeutic Electronic Co., Ltd. |