CN102564919B - Flow chamber component, flow chamber blockage removing method and sample analyzer - Google Patents
Flow chamber component, flow chamber blockage removing method and sample analyzer Download PDFInfo
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- CN102564919B CN102564919B CN201010619812.6A CN201010619812A CN102564919B CN 102564919 B CN102564919 B CN 102564919B CN 201010619812 A CN201010619812 A CN 201010619812A CN 102564919 B CN102564919 B CN 102564919B
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Abstract
The invention discloses a flow chamber component, a flow chamber blockage removing method and a sample analyzer. The flow chamber component comprises a flow chamber, a rectifying chamber, a first adding channel, a second adding channel, liquid adding containers, a first liquid channel, a second liquid channel and liquid containers, wherein the flow chamber is provided with a flow chamber outlet; the rectifying chamber is communicated with the flow chamber; and the rectifying chamber and the flow chamber outlet are respectively connected with the corresponding liquid adding containers by the first adding channel and the second channel, and are respectively connected with the corresponding liquid containers by the first liquid channel and the second liquid channel. When blockage removing is needed for the flow chamber, a liquid enters the flow chamber by the flow chamber outlet, and washes the flow chamber reversely, and the blockage is removed by the rectifying chamber, so that the blockage removing operation and daily maintenance for the flow chamber are convenient.
Description
Technical field
The invention relates to a kind of flow chamber assembly and sample analyser.
Background technology
In flow cytometer, flow chamber assembly is the important place that carries out input.Flow chamber assembly generally comprises flow chamber, specimen needle, rectification chamber, connect the passage of each element and control the operation valve of each passage break-make.The sample flow that detects use is penetrated by specimen needle, by outer field sheath stream parcel, constrain in a narrower and comparatively stable region in flow chamber, as the middle section of flow chamber, by hot spot, irradiate the particle in sample flow, collect the various types of scattered light signals that produce, in conjunction with biological property, signal is carried out to statistic of classification, obtain having the parameter information of clinical meaning.
Generally speaking, in order to obtain more stable sample flow and scattered light signal, the latus rectum of flow chamber is less, be generally 0.1～0.4mm, but due to the complicated component in sample flow and sheath fluid, the reactant that has blood sample and reagent, also may have in blood collection procedure, bring as foreign material such as skin histology, cotton swab silks, analyser puncture test tube is inhaled sample and the cap test tube chip that produces or the impurity of other form etc.Therefore,, in using the process of flow cytometer, may there is situation about stopping up in flow chamber.
Once flow chamber stops up, comprise micro-stifledly, sample flow just can not be stablized effective formation, and scattered light signal also there will be distortion, thereby produces wrong clinical diagnosis information, in this case, just safeguards accordingly and gets rid of obstruction in needs flow chamber.
At present, for the maintenance of flow chamber, can automatically cleaning reagent be assigned to the porch of flow chamber by analyser, by syringe or pressure-driven, make cleaning reagent enter flow chamber, cleaning reagent can carry out chemical reaction with some specific materials, plays the effect of unimpeded and cleaning.But, for other material, as cap test tube chip etc., cleaning reagent can not guarantee to play the effect of chemolysis, and in the situation that stopping up appears completely in flow chamber, cleaning reagent can not guarantee effectively to enter into the position that flow chamber stops up, therefore, conventionally need the artificial instruments such as syringe that use, from the Way out propelling movement liquid of flow chamber, carry out unimpeded.Such unimpeded operation, need to open analyser, and some relevant passages or valve are disconnected, and can carry out, and inconvenience therefore operates.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of flow chamber assembly, unimpeded method and sample analyser of being convenient to flow chamber unimpeded and maintenance is provided.
For achieving the above object, the present invention has adopted following technical scheme: a kind of flow chamber assembly, comprise flow chamber, rectification chamber, first adds passage, second adds passage, liquid adds container, first liquid passage, second liquid passage and liquid container, described flow chamber has flow chamber outlet, described rectification chamber and described flow chamber are communicated with, described rectification chamber adds passage to add container to be connected with described liquid by described first, outlet is connected with corresponding liquid container with second liquid passage by described first liquid passage respectively with flow chamber in described rectification chamber, while needing unimpeded, make the conducting of described first liquid passage, liquid flows into described flow chamber outlet, flow chamber, rectification chamber, first liquid passage and with the liquid container of the corresponding connection of first liquid passage.When unimpeded, need to utilize pressure to control the direction of liquid flow, as provided this pressure by power source is set, this pressure is as normal pressure or negative pressure, and this power source can provide pressure to drive the power source of liquid flow as syringe or other.
Further, while needing unimpeded, make described first liquid passage and the conducting of second liquid passage, liquid flow into described second liquid passage, flow chamber outlet, flow chamber, rectification chamber, first liquid passage and with the liquid container of the corresponding connection of first liquid passage.
Further, described flow chamber assembly also comprises that second adds passage, described flow chamber outlet adds passage to add container to be connected with described liquid by described second, while needing unimpeded, make described second to add passage and the conducting of first liquid passage, liquid flow into described second add passage, flow chamber outlet, flow chamber, rectification chamber, first liquid passage and with the liquid container of the corresponding connection of first liquid passage.
Further, described rectification chamber has rectification chamber interface, and the latus rectum of described rectification chamber interface is greater than the latus rectum of described flow chamber, and described first liquid passage connects described rectification chamber interface.Because the latus rectum of rectification chamber interface is larger, be convenient to discharge the tamper of flow chamber.
Further, described first adds passage to connect described rectification chamber interface.
Further, described liquid container has two, be respectively first liquid container and second liquid container, described first liquid container connects described rectification chamber interface by described first liquid passage, and described second liquid container connects described flow chamber by described second liquid passage and exports.
Further, described first liquid container and second liquid container are communicated with.
Further, described flow chamber assembly also comprises specimen needle, and described specimen needle stretches into described rectification chamber and over against described flow chamber.
Further, described first adds passage, second to add passage, first liquid passage and second liquid passage to be respectively equipped with the first access control device, the second access control device, first liquid controller and the second liquid controller for control channel break-make.By controller is set, be convenient to the break-make of each passage automatically to control.
Further, in the time of need to detecting, make described first to add passage and the conducting of second liquid passage, liquid flow into described first add passage, rectification chamber, flow chamber, flow chamber outlet, second liquid passage and with the liquid container of the corresponding connection of second liquid passage.
A sample analyser, comprises described flow chamber assembly.
A unimpeded method for flow chamber assembly, described flow chamber assembly comprises flow chamber and the rectification chamber being interconnected, described flow chamber has flow chamber outlet, comprises the steps:
A) drive liquid to enter described flow chamber from described flow chamber outlet, the tamper that stops up described flow chamber is poured to described rectification chamber; B) drive described tamper to discharge from described rectification chamber.
Further, described step a) in, utilize normal pressure to drive described liquid; Described step b), in, utilize negative pressure to drive described tamper.
Further, a) the front step c that also comprises of described step): emptying described flow chamber and rectification chamber.
Further, described step b), also comprise steps d): clean, pour into described flow chamber and rectification chamber.
Further, described step a) comprises the steps: a1) make described rectification chamber and flow chamber outlet by first, add passage and second to add passage to be connected liquid respectively to add container, described flow chamber is exported and by first liquid passage, be connected corresponding liquid container with second liquid passage respectively with rectification chamber; A2) make described second to add passage and the conducting of first liquid passage, utilize normal pressure to drive described liquid to add the liquid in container to enter described flow chamber from described flow chamber outlet; Described step b) in, make described first liquid passage and the conducting of second liquid passage, utilize negative pressure to drive tamper to discharge from described rectification chamber.
The invention has the beneficial effects as follows: while needing the unimpeded of flow chamber, make liquid be exported and be entered flow chamber by flow chamber, counterflush is carried out in flow chamber, and discharge tamper by rectification chamber, facilitated unimpeded operation and the daily maintaining of flow chamber.
Accompanying drawing explanation
Fig. 1 is the structural representation of flow chamber assembly the first embodiment;
Fig. 2 is the structural representation of flow chamber assembly the second embodiment.
Embodiment
Below by embodiment, by reference to the accompanying drawings the present invention is described in further detail.
As shown in Figure 1, present embodiment flow chamber component application is in sample analyser, and it comprises that flow chamber 1, rectification chamber 2, specimen needle 3, liquid add container 4, first liquid container 6 and second liquid container 5.Flow chamber 1 has flow chamber entrance 11 and flow chamber outlet 12.Rectification chamber 2 and flow chamber entrance 11 are communicated with, and this rectification chamber 2 has rectification chamber interface 201.Specimen needle 3 stretches into rectification chamber 2 and over against flow chamber 1.Liquid adds container 4 to add passage 7 to be connected with rectification chamber interface 201 by first, and this first adds passage 7 to be provided with the first access control device 43 for control channel break-make.Liquid adds container 4 to add passage 8 and flow chamber outlet 12 to be connected by second, and this second adds passage 8 to be provided with the second access control device 44 for control channel break-make.First liquid container 6 is connected with rectification chamber interface 201 by first liquid passage 9, and this first liquid passage 9 is provided with the first liquid controller 42 for control channel break-make.Second liquid container 5 is connected with flow chamber outlet 12 by second liquid path 10, and this second liquid path 10 is provided with the second liquid controller 45 for control channel break-make.
While carrying out pattern detection, can open the first access control device 43 and second liquid controller 45, liquid adds the reagent in container 4 to drive by normal pressure, from rectification chamber interface 201, enters rectification chamber 2, and forms stable laminar flow in rectification chamber 2 and flow chamber 1; Sample to be tested adds rectification chamber 2 from specimen needle 3, after 2 rectifications of overcommutation chamber, flow into flow chamber 1, forms the stable sample flow of being wrapped up by laminar flow.Sample flow is detected when by flow chamber 1, and second liquid container 5 is discharged and be collected into the waste liquid after having detected from flow chamber outlet 12, and can be discharged to outside analyser by second liquid container 5.Certainly, also first liquid container 6 and second liquid container 5 can be communicated with, unified by first liquid container 6 collection waste liquids, collect a certain amount of after, under normal pressure drives, the waste liquid in first liquid container is discharged to outside analyser, in such cases, analyser can only arrange a waste liquid mouth, is convenient to control.
When flow chamber 1 is blocked while needing unimpeded, first open first liquid controller 42 and second liquid controller 45, flow chamber 1 and rectification chamber 2 is emptying; Then, close second liquid controller 45 and open the second access control device 44, at liquid, add under the normal pressure of container 4 and the negative pressure effect of first liquid container 6, liquid adds passage 8 to enter flow chamber 1 through flow chamber outlet 12 along second, counterflush is carried out in flow chamber 1, and the liquid after flushing is discharged to first liquid container 6 by rectification chamber interface 201; Then close the second access control device 44 and open second liquid controller 45, under the negative pressure effect of first liquid container 6, rectification chamber 2 is emptying, the tamper developing is discharged to first liquid container 6 by rectification chamber interface 201; Finally.Clean, pour into flow chamber 1 and rectification chamber 2, whole flow chamber assembly is resetted.
While needing the passage (as second liquid passage) in exit, flow chamber and controller (as second liquid controller) to clean, can directly open and add the second access control device 44 and second liquid controller 45, directly utilize liquid to add the liquid in container to rinse this passage and controller, do not need through flow chamber, realized the direct cleaning in exit, flow chamber, the waste liquid having reduced in passage and controller is residual.
As shown in Figure 2, it is the second embodiment of flow chamber assembly, this embodiment is with the key distinction of the first embodiment: do not arrange and be connected flow chamber outlet 12 and liquid adds second of container to add passage, other structure of this embodiment can be identical with the first embodiment.
During cleaning, make first to add passage 7 and the conducting of second liquid path 10, liquid can flow into second and add passage 7, rectification chamber 2, flow chamber 1, flow chamber outlet 12, second liquid path 10 and second liquid container 5.During unimpeded, make first liquid passage 9 and the conducting of second liquid path 10, to 5 pressurizations of second liquid container, under pressure, liquid can flow into second liquid path 10, flow chamber outlet 12, flow chamber 1, rectification chamber 2, first liquid passage 9 and first liquid container 6.
For flow chamber assembly, comprise flow chamber and rectification chamber, this flow chamber has flow chamber outlet, this rectification chamber is communicated with this flow chamber, and the outlet of rectification chamber and flow chamber is all connected with corresponding liquid container, and rectification chamber is also connected with liquid and adds container, in the time of need to detecting, under pressure-driven, liquid adds the liquid in container flow through rectification chamber, flow chamber and flow chamber outlet, finally by the liquid container being connected with this flow chamber outlet, is collected; While needing unimpeded, under pressure-driven, liquid flow through flow chamber outlet, flow chamber and rectification chamber, finally collected by the liquid container being connected with this rectification chamber.While needing unimpeded, liquid enters flow chamber from flow chamber outlet, realize the counterflush of flow chamber, this liquid can be to add container to export by the liquid being connected with flow chamber outlet, also can be to be exported by the liquid container being connected with flow chamber outlet, or be exported with the container that flow chamber outlet is connected by other.
For flow chamber assembly, flow chamber outlet all can add container by adding passage to be connected liquid with rectification chamber.Liquid adds container can only have one, and it adds passage to realize respectively and being connected of flow chamber outlet and rectification chamber by two; Liquid adds container also can have two, and these two liquid add container to add passage to connect respectively flow chamber outlet and rectification chamber by two.Liquid container can have two, and these two liquid containers connect respectively rectification chamber by two fluid passages and flow chamber exports, and these two liquid containers can independently arrange, and also can pass through channel connection; Liquid container also can only have one, i.e. flow chamber outlet is connected this liquid container by fluid passage respectively with rectification chamber.Rectification chamber can have a rectification chamber interface, and this rectification chamber interface can connect first liquid container and liquid adds container simultaneously; Rectification chamber also can have two rectification chamber interfaces, and two rectification chamber interfaces connect respectively first liquid container and liquid adds container.The latus rectum of rectification chamber interface can be greater than the latus rectum of flow chamber, thereby is convenient to discharge tamper, and this latus rectum refers to the size that the inside of this interface or flow chamber can be passed through for liquid.On each passage, all can be provided for the controller of control channel break-make, this controller is as solenoid valve, pneumatic pilot-valve, or other can play the structure of passage break-make control action.
Flow chamber assembly can be applied on flow cytometer, on blood cell analyzer, or other flow chamber and rectification chamber need to be set and sample analyser that the sample flow by flow chamber is detected on.
A kind of unimpeded method of flow chamber assembly, flow chamber assembly comprises flow chamber and the rectification chamber being interconnected, flow chamber has flow chamber outlet, comprises the steps: a) to drive liquid to flow into flow chamber from flow chamber outlet, and the tamper that stops up described flow chamber is poured to rectification chamber; B) drive tamper to discharge from rectification chamber.For the ease of fully rinsing tamper, the method can also be positioned at a) front step c of described step): flow chamber and rectification chamber is emptying.For the ease of carrying out detection next time operation, the method can also comprise be positioned at described step b) after steps d): clean, perfusion flow chamber and rectification chamber, whole flow chamber assembly resetted.
Step a) can comprise: a1) make the outlet of rectification chamber and flow chamber by first, add passage and second to add passage to be connected liquid respectively and add container, flow chamber is exported and by first liquid passage, be connected corresponding liquid container with second liquid passage respectively with rectification chamber; A2) make second to add passage and the equal conducting of first liquid passage, utilize liquid to add the normal pressure of container to drive liquid to enter flow chamber from flow chamber outlet; Step b) in, make first liquid passage and the equal conducting of second liquid passage, utilize the negative pressure of the liquid container being connected with rectification chamber to drive tamper to discharge from rectification chamber.
Step a) also can comprise: a1) make rectification chamber add passage to connect liquid by first and add container, make flow chamber outlet by first liquid passage, be connected corresponding liquid container with second liquid passage respectively with rectification chamber; A2) make first liquid passage and the equal conducting of second liquid passage, utilize the normal pressure of the liquid container being connected with flow chamber outlet to drive liquid to enter flow chamber from flow chamber outlet; Described step b), in, utilize the negative pressure of the liquid container being connected with rectification chamber to drive tamper to discharge from rectification chamber.
Above content is in conjunction with concrete embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.
Claims (15)
1. a flow chamber assembly, comprise flow chamber and rectification chamber, described flow chamber has flow chamber outlet, described rectification chamber and described flow chamber are communicated with, it is characterized in that: also comprise that first adds passage, liquid adds container, first liquid passage, second liquid passage and liquid container, described rectification chamber adds passage to add container to be connected with described liquid by described first, outlet is connected with corresponding liquid container with second liquid passage by described first liquid passage respectively with flow chamber in described rectification chamber, while needing unimpeded, make the conducting of described first liquid passage, liquid flows into described flow chamber outlet, flow chamber, rectification chamber, first liquid passage and with the liquid container of the corresponding connection of first liquid passage, in the time of need to detecting, under pressure-driven, described liquid adds the liquid in container flow through described rectification chamber, flow chamber, flow chamber outlet and second liquid passage, is finally collected with the liquid container of the corresponding connection of described second liquid passage.
2. flow chamber assembly as claimed in claim 1, it is characterized in that: while needing unimpeded, make described first liquid passage and the equal conducting of second liquid passage, liquid flow into described second liquid passage, flow chamber outlet, flow chamber, rectification chamber, first liquid passage and with the liquid container of the corresponding connection of first liquid passage.
3. flow chamber assembly as claimed in claim 1, it is characterized in that: also comprise that second adds passage, described flow chamber outlet adds passage to add container to be connected with described liquid by described second, while needing unimpeded, make described second to add passage and the equal conducting of first liquid passage, liquid flow into described second add passage, flow chamber outlet, flow chamber, rectification chamber, first liquid passage and with the liquid container of the corresponding connection of first liquid passage.
4. the flow chamber assembly as described in any one in claim 1-3, is characterized in that: described rectification chamber has rectification chamber interface, and the latus rectum of described rectification chamber interface is greater than the latus rectum of described flow chamber, and described first liquid passage connects described rectification chamber interface.
5. flow chamber assembly as claimed in claim 4, is characterized in that: described first adds passage to connect described rectification chamber interface.
6. flow chamber assembly as claimed in claim 4, it is characterized in that: described liquid container has two, be respectively first liquid container and second liquid container, described first liquid container connects described rectification chamber interface by described first liquid passage, and described second liquid container connects described flow chamber by described second liquid passage and exports.
7. flow chamber assembly as claimed in claim 6, is characterized in that: described first liquid container and second liquid container are communicated with.
8. the flow chamber assembly as described in any one in claim 1-3, is characterized in that: also comprise specimen needle, described specimen needle stretches into described rectification chamber and over against described flow chamber.
9. the flow chamber assembly as described in any one in claim 1-3, is characterized in that: each passage is equipped with the controller for control channel break-make.
10. a sample analyser, is characterized in that: comprise the flow chamber assembly described in any one in claim 1-9.
The unimpeded method of 11. 1 kinds of flow chamber assemblies, described flow chamber assembly comprises flow chamber and the rectification chamber being interconnected, described flow chamber has flow chamber outlet, it is characterized in that: comprise the steps:
A) drive liquid to enter described flow chamber from described flow chamber outlet, the tamper that stops up described flow chamber is poured to described rectification chamber;
B) drive described tamper to discharge from described rectification chamber.
The unimpeded method of 12. flow chamber assemblies as claimed in claim 11, is characterized in that: described step a) in, utilize normal pressure to drive described liquid; Described step b), in, utilize negative pressure to drive described tamper.
The unimpeded method of 13. flow chamber assemblies as claimed in claim 11, is characterized in that: described step is the front step c that also comprises a)): emptying described flow chamber and rectification chamber.
The unimpeded method of 14. flow chamber assemblies as claimed in claim 13, is characterized in that: described step b), also comprise steps d): clean, pour into described flow chamber and rectification chamber.
The unimpeded method of 15. flow chamber assemblies as described in any one in claim 11-14, is characterized in that: described step a) comprises the steps:
A1) make the outlet of described rectification chamber and flow chamber by first, add passage and second to add passage to be connected liquid respectively and add container, described flow chamber is exported and by first liquid passage, be connected corresponding liquid container with second liquid passage respectively with rectification chamber;
A2) make described second to add passage and the equal conducting of first liquid passage, utilize normal pressure to drive described liquid to add the liquid in container to enter described flow chamber from described flow chamber outlet;
Described step b) in, make described first liquid passage and the equal conducting of second liquid passage, utilize negative pressure to drive tamper to discharge from described rectification chamber.
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| CN201010619812.6A CN102564919B (en) | 2010-12-31 | 2010-12-31 | Flow chamber component, flow chamber blockage removing method and sample analyzer |
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| CN201010619812.6A CN102564919B (en) | 2010-12-31 | 2010-12-31 | Flow chamber component, flow chamber blockage removing method and sample analyzer |
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| CN102564919B true CN102564919B (en) | 2014-03-19 |
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| CN103487361B (en) * | 2013-10-15 | 2015-08-19 | 爱威科技股份有限公司 | A kind of counting chamber pump valve system and control method thereof |
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| US7656146B2 (en) * | 2006-11-02 | 2010-02-02 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Particle analyzer based on sheath flow impedance method |
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Application publication date: 20120711 Assignee: Shenzhen Mindray Animal Medical Technology Co.,Ltd. Assignor: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd. Contract record no.: X2022440020009 Denomination of invention: Flow chamber assembly, blockage removal method for flow chamber assembly, and sample analyzer Granted publication date: 20140319 License type: Common License Record date: 20220804 |