CN109772822A - A kind of stream type cell analyzer automatic washing system and its working method - Google Patents
A kind of stream type cell analyzer automatic washing system and its working method Download PDFInfo
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- CN109772822A CN109772822A CN201811651055.3A CN201811651055A CN109772822A CN 109772822 A CN109772822 A CN 109772822A CN 201811651055 A CN201811651055 A CN 201811651055A CN 109772822 A CN109772822 A CN 109772822A
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- cleaning
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- needle
- solenoid valve
- reaction cup
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Abstract
The invention discloses a kind of stream type cell analyzer automatic washing systems, belong to clinical medicine physiology, biochemistry, the special-purpose multifunctional device for fast detecting of pathological examination and technical field, including washing device, the first detergent line, the first solenoid valve, the first cleaning branch, second solenoid valve, forebay, after-bay, gem hole, the first inlet tube, the first outlet tube, third solenoid valve, sampling needle, swab, the 4th solenoid valve, the second cleaning branch, third cleaning branch, one group of reaction cup cleaning needle, one group of aspirating needle, the second drain pipe and waste liquid outlet.Stream type cell analyzer automatic washing system of the present invention, by the way that reasonable cleaning agent flow pipe is arranged, it is able to carry out the cleaning of cytoanalyze each unit, improve the efficiency of cleaning, and improve the service efficiency of cleaning agent, it can guarantee the cleaning effect of cytoanalyze, it is safe and reliable.
Description
Technical field
The invention belongs to clinical medicine physiology, biochemistry, the special-purpose multifunctional device for fast detecting of pathological examination and technology necks
Domain, in particular to a kind of stream type cell analyzer automatic washing system.The invention further relates to a kind of stream type cell analyzer is complete
The working method of automatic cleaning system.
Background technique
Flow cytometer (flow cytometer, FCM) is a kind of cell analysis instrument according to light scattering theory.When sharp
When illumination is mapped to each cell individually arranged flowed through in flow chamber, due to the physical characteristic of cell, some light is from thin
Born of the same parents are upper to be scattered through different angles.Wherein, the light intensity of Small angle forward scattering light can reflect cell volume；Large-angle scattered light
Light intensity can reflect the information of nucleus complexity and cell granulations；And the light intensity of side scattered light can with reacting cells film,
Nuclear membrane, cytoplasmic variation to classify to cell, and are analyzed cell DAN, RNA.
Optical detection apparatus is the important component for carrying out cell analysis, therefore must also be carried out with dilution clearly after analyzing measurement
It washes.So cleaning system is the essential component of cytoanalyze, mainly it is made of the pipeline of transport dilution, with stream
Dilution is delivered to component to be cleaned by power source drive diluent flow and carried out by the connection of body motion-promotion force source, cleaning system
Cleaning.
However, its each functional unit of traditional cytoanalyze is since cleaning requirement is different, so cleaning system is mutually only
Vertical, structure is complicated for dilution transport network, causes apparatus structure complicated, instrument cost is high, and cleaning efficiency and utilization rate are low.
Summary of the invention
Goal of the invention: in order to overcome the above deficiency, the object of the present invention is to provide a kind of stream type cell analyzer is full-automatic
Cleaning system provides a set of comprehensive system for carrying out cytoanalyze cleaning.The present invention also provides a kind of flow cytometries
The working method of instrument automatic washing system, working principle is simple and easy, and required manpower is few, can carry out cell point automatically
The cleaning of analyzer.
Technical solution: a kind of stream type cell analyzer automatic washing system, including washing device, the first detergent line,
First solenoid valve, the first cleaning branch, second solenoid valve, forebay, after-bay, gem hole, the first inlet tube, the first outlet tube, the
Three solenoid valves, sampling needle, swab, the 4th solenoid valve, the second cleaning branch, third clean branch, one group of reaction cup cleaning needle, one
One end of group aspirating needle, the second drain pipe and waste liquid outlet, first detergent line is arranged in washing device, and described the
One solenoid valve is arranged in the first detergent line, and the first cleaning branch is connect with the first detergent line, second electromagnetism
Valve setting is on the first cleaning branch road, and the first cleaning branch is connect by the first inlet tube with after-bay, and the forebay passes through
Gem hole is connected to after-bay, and the second cleaning branch is connect by the first outlet tube with swab, the third solenoid valve setting
On the second cleaning branch road, the swab is set on sampling needle, and the third cleaning branch is connect with swab, the 4th electricity
Magnet valve setting is on third cleaning branch road, and the third cleaning branch is connected to one group of reaction cup cleaning needle, and the reaction cup is clear
Wash needle may be provided in reaction cup by lifting structure, and one group of aspirating needle is arranged in reaction cup, and one group of aspirating needle
Cleaning solution in reaction cup can be sucked out to the second drain pipe, second drain pipe is connected to waste liquid outlet, the waste liquid
Waste liquid can be discharged for outlet.
Further, above-mentioned stream type cell analyzer automatic washing system is connected in first detergent line
4th cleans branch, and the 5th solenoid valve is connected on the 4th cleaning branch, and the end of the 4th cleaning branch is connected with
Optical detection apparatus.
Further, above-mentioned stream type cell analyzer automatic washing system is connected with cleaning on the washing device
Liquid supplement pipe, the cleaning solution supplement and are connected with cleaning solution supplement tank on pipe, and the cleaning solution supplement pipe is equipped with metering pool.
Further, above-mentioned stream type cell analyzer automatic washing system, the cleaning solution supplement tank and flushing liquor
There is cleaning agent in tank.
The present invention also provides a kind of working methods of stream type cell analyzer automatic washing system, comprising the following steps:
1) cleaning agent in washing device flow to the first cleaning branch by the first detergent line；
2) cleaning agent flows into after-bay along the first cleaning branch；
3) cleaning agent in after-bay flow to forebay；
4) cleaning agent in forebay flow to the second cleaning branch by the first outlet tube, and flows into swab；
5) swab carries out the cleaning of sampling needle；
6) cleaning agent in swab cleans branch by third and flow in one group of reaction cup cleaning needle；
7) one group of reaction cup cleaning needle is protruded into reaction cup by lifting structure, carries out the cleaning of reaction cup；
8) cleaning agent is sucked out one group of aspirating needle in reaction cup, is arranged cleaning agent by the second drain pipe and waste liquid outlet
Out.
The present invention also provides a kind of working methods of stream type cell analyzer automatic washing system, further include following step
It is rapid:
9) when the cleaning agent in washing device is lower than setting value, pipe is supplemented for the cleaning in cleaning solution supplement tank by cleaning solution
Agent is supplemented into washing device, and metering pool metering is filled with the volume of the cleaning agent of washing device.
The present invention also provides a kind of working methods of stream type cell analyzer automatic washing system, further include following step
It is rapid:
10) cleaning agent in the first detergent line is flow in the 4th cleaning branch, carries out the cleaning of optical detection apparatus.
Above-mentioned technical proposal can be seen that the invention has the following beneficial effects: flow cytometries of the present invention
Instrument automatic washing system is able to carry out the clear of cytoanalyze each unit by the way that reasonable cleaning agent flow pipe is arranged
It washes, improves the efficiency of cleaning, and improve the service efficiency of cleaning agent, can guarantee the cleaning effect of cytoanalyze,
It is safe and reliable, and cleaning agent is not fully stored in cleaning fluid tank, using the cleaning solution supplement being separately provided
Tank carries out the storage of cleaning agent, can quantify the additional clean agent into cleaning fluid tank, cleaning agent is prevented to be contaminated in use
Or it is rotten.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of stream type cell analyzer automatic washing system of the present invention.
In figure: washing device 1, the first detergent line 2, the first solenoid valve 3, first cleaning branch 4, second solenoid valve 5, preceding
Pond 6, after-bay 7, gem hole 8, the first inlet tube 9, the first outlet tube 10, third solenoid valve 11, sampling needle 12, swab the 13, the 4th
Solenoid valve 14, second cleans branch 15, third cleans 16, one groups of branch, 17, one groups of reaction cup cleaning needle aspirating needle 18, second row
Liquid pipe 19, waste liquid outlet 20, four clean branch 21, the 5th solenoid valve 22, optical detection apparatus 23, cleaning solution supplement pipe 24,
Cleaning solution supplements tank 25, metering pool 26.
Specific embodiment
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to is used to explain the present invention, and is not considered as limiting the invention.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", " clockwise ", " inverse
The orientation or positional relationship of the instructions such as hour hands " is to be based on the orientation or positional relationship shown in the drawings, and is merely for convenience of description originally
Invention and simplified description, rather than the device or element of indication or suggestion meaning must have a particular orientation, with specific side
Position construction and operation, therefore be not considered as limiting the invention.
In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance
Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include one or more of the features.In the description of the present invention, unless otherwise indicated, the meaning of " plurality " is two
It is a or more than two, unless otherwise restricted clearly.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " fixation " etc.
Term shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can be machine
Tool connection, is also possible to be electrically connected；It can be directly connected, two members can also be can be indirectly connected through an intermediary
Connection inside part.For the ordinary skill in the art, above-mentioned term can be understood in this hair as the case may be
Concrete meaning in bright.
In the present invention unless specifically defined or limited otherwise, fisrt feature second feature "upper" or "lower"
It may include that the first and second features directly contact, also may include that the first and second features are not direct contacts but pass through it
Between other characterisation contact.Moreover, fisrt feature includes the first spy above the second feature " above ", " above " and " above "
Sign is right above second feature and oblique upper, or is merely representative of first feature horizontal height higher than second feature.Fisrt feature exists
Second feature " under ", " lower section " and " following " include that fisrt feature is directly below and diagonally below the second feature, or is merely representative of
First feature horizontal height is less than second feature.
Embodiment
Stream type cell analyzer automatic washing system as shown in Figure 1, including washing device 1, the first detergent line 2,
First solenoid valve 3, first cleaning branch 4, second solenoid valve 5, forebay 6, after-bay 7, gem hole 8, the first inlet tube 9, first go out
Liquid pipe 10, third solenoid valve 11, sampling needle 12, swab 13, the 4th solenoid valve 14, second cleaning branch 15, third clean branch
16, one group of reaction cup cleaning needle, 17, one groups of aspirating needles 18, the second drain pipe 19 and waste liquid outlet 20, first cleaning pipe
The one end on road 2 is arranged in washing device 1, and first solenoid valve 3 is arranged in the first detergent line 2, first cleaning
Branch 4 is connect with the first detergent line 2, and the setting of second solenoid valve 5 is on the first cleaning branch 4, the first cleaning branch
Road 4 is connect by the first inlet tube 9 with after-bay 7, and the forebay 6 is connected to by gem hole 8 with after-bay 7, the second cleaning branch
Road 15 is connect by the first outlet tube 10 with swab 13, and the setting of third solenoid valve 11 is described on the second cleaning branch 15
Swab 13 is set on sampling needle 12, and the third cleaning branch 16 is connect with swab 13, and the 4th solenoid valve 14 setting exists
Third is cleaned on branch 16, and the third cleaning branch 16 is connected to one group of reaction cup cleaning needle 17, the reaction cup cleaning needle
17 may be provided in reaction cup by lifting structure, and one group of aspirating needle 18 is arranged in reaction cup, and one group of aspirating needle
18 the cleaning solution in reaction cup can be sucked out to the second drain pipe 19, and second drain pipe 19 is connected to waste liquid outlet 20,
Waste liquid can be discharged for the waste liquid outlet 20.Wherein, it is connected with the 4th cleaning branch 21 in the first detergent line 2, described the
The 5th solenoid valve 22 is connected on four cleaning branches 21, the end of the 4th cleaning branch 21 is connected with optical detection apparatus
23.In addition, being connected with cleaning solution supplement pipe 24 on washing device 1, cleaning solution supplement is connected on the cleaning solution supplement pipe 24
Tank 25, the cleaning solution supplement pipe 24 are equipped with metering pool 26.There is cleaning agent in cleaning solution supplement tank 25 and washing device 1.
A kind of working method of stream type cell analyzer automatic washing system of the present invention, comprising the following steps:
1) cleaning agent in washing device 1 flow to the first cleaning branch 4 by the first detergent line 2；
2) cleaning agent flows into after-bay 7 along the first cleaning branch 4；
3) cleaning agent in after-bay 7 flow to forebay 6；
4) cleaning agent in forebay 6 flow to the second cleaning branch 15 by the first outlet tube 10, and flows into swab 13；
5) swab 13 carries out the cleaning of sampling needle 12；
6) cleaning agent in swab 13 cleans branch 16 by third and flow in one group of reaction cup cleaning needle 17；
7) one group of reaction cup cleaning needle 17 is protruded into reaction cup by lifting structure, carries out the cleaning of reaction cup；
8) cleaning agent is sucked out one group of aspirating needle 18 in reaction cup, will be cleaned by the second drain pipe 19 and waste liquid outlet 20
Agent discharge.
It is above-mentioned only to have a certain amount of cleaning agent in washing device 1 in the washing device use process, in washing device 1
When cleaning agent is lower than setting value, following step additional clean agent can be used:
9) when the cleaning agent in washing device 1 is lower than setting value, pipe 24 is supplemented by cleaning solution and supplements cleaning solution in tank 25
Cleaning agent be supplemented into washing device 1, metering pool 26 measure be filled with washing device 1 cleaning agent volume.
Above-mentioned cleaning process successively flows for cleaning agent is cleaned, and can also pass through transfer tube in the first detergent line 2
Another branch is separated, the cleaning of optical detection apparatus 23 is carried out, specifically comprises the following steps:
10) cleaning agent in the first detergent line 2 is flow in the 4th cleaning branch 21, carries out the cleaning of optical detection apparatus 23.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the principle of the present invention, several improvement can also be made, these improvement also should be regarded as of the invention
Protection scope.
Claims (7)
1. a kind of stream type cell analyzer automatic washing system, it is characterised in that: including washing device (1), the first cleaning pipe
Road (2), the first solenoid valve (3), first cleaning branch (4), second solenoid valve (5), forebay (6), after-bay (7), gem hole (8),
First inlet tube (9), the first outlet tube (10), third solenoid valve (11), sampling needle (12), swab (13), the 4th solenoid valve
(14), the second cleaning branch (15), third cleaning branch (16), one group of reaction cup cleaning needle (17), one group of aspirating needle (18), the
Two drain pipes (19) and waste liquid outlet (20), one end setting of first detergent line (2) is in washing device (1), institute
It states the first solenoid valve (3) to be arranged on the first detergent line (2), first cleaning branch (4) and the first detergent line (2) are even
It connects, in the first cleaning branch (4), first cleaning branch (4) passes through the first inlet tube for second solenoid valve (5) setting
(9) it is connect with after-bay (7), the forebay (6) is connected to by gem hole (8) with after-bay (7), and second cleaning branch (15) is logical
It crosses the first outlet tube (10) to connect with swab (13), third solenoid valve (11) setting is in the second cleaning branch (15), institute
It states swab (13) to be set on sampling needle (12), third cleaning branch (16) connect with swab (13), the 4th electromagnetism
In third cleaning branch (16), third cleaning branch (16) connects with one group of reaction cup cleaning needle (17) for valve (14) setting
Logical, the reaction cup cleaning needle (17) may be provided in reaction cup by lifting structure, and one group of aspirating needle (18) setting exists
In reaction cup, and one group of aspirating needle (18) cleaning solution in reaction cup can be sucked out to the second drain pipe (19), and described second
Drain pipe (19) is connected to waste liquid outlet (20), and waste liquid can be discharged for the waste liquid outlet (20).
2. stream type cell analyzer automatic washing system according to claim 1, it is characterised in that: first cleaning
It is connected with the 4th cleaning branch (21) on pipeline (2), is connected with the 5th solenoid valve (22), institute in the 4th cleaning branch (21)
The end for stating the 4th cleaning branch (21) is connected with optical detection apparatus (23).
3. stream type cell analyzer automatic washing system according to claim 1, it is characterised in that: the washing device
(1) it is connected with cleaning solution supplement pipe (24) on, cleaning solution supplement tank (25) is connected on the cleaning solution supplement pipe (24), it is described
Cleaning solution supplement pipe (24) is equipped with metering pool (26).
4. stream type cell analyzer automatic washing system according to claim 1 or 3, it is characterised in that: the cleaning
There is cleaning agent in liquid supplement tank (25) and washing device (1).
5. the working method of stream type cell analyzer automatic washing system according to claim 1-3, special
Sign is: the following steps are included:
1) cleaning agent in washing device (1) flow to the first cleaning branch (4) by the first detergent line (2)；
2) cleaning agent flows into after-bay (7) along the first cleaning branch (4)；
3) cleaning agent in after-bay (7) flow to forebay (6)；
4) cleaning agent in forebay (6) flow to the second cleaning branch (15) by the first outlet tube (10), and flows into swab (13)；
5) swab (13) carries out the cleaning of sampling needle (12)；
6) cleaning agent in swab (13) is flow in one group of reaction cup cleaning needle (17) by third cleaning branch (16)；
7) one group of reaction cup cleaning needle (17) is protruded into reaction cup by lifting structure, carries out the cleaning of reaction cup；
8) cleaning agent is sucked out one group of aspirating needle (18) in reaction cup, passes through the second drain pipe (19) and waste liquid outlet (20)
Cleaning agent is discharged.
6. the working method of stream type cell analyzer automatic washing system according to claim 3, it is characterised in that: also
The following steps are included:
9) when the cleaning agent in washing device (1) is lower than setting value, pipe (24) is supplemented by cleaning solution, cleaning solution is supplemented into tank
(25) cleaning agent in is supplemented into washing device (1), and metering pool (26) metering is filled with the body of the cleaning agent of washing device (1)
Product.
7. the working method of stream type cell analyzer automatic washing system according to claim 2, it is characterised in that: also
The following steps are included:
10) cleaning agent in the first detergent line (2) is flow in the 4th cleaning branch (21), carries out optical detection apparatus (23)
Cleaning.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811651055.3A CN109772822A (en) | 2018-12-31 | 2018-12-31 | A kind of stream type cell analyzer automatic washing system and its working method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811651055.3A CN109772822A (en) | 2018-12-31 | 2018-12-31 | A kind of stream type cell analyzer automatic washing system and its working method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109772822A true CN109772822A (en) | 2019-05-21 |
Family
ID=66499767
Family Applications (1)
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| CN201811651055.3A Pending CN109772822A (en) | 2018-12-31 | 2018-12-31 | A kind of stream type cell analyzer automatic washing system and its working method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112536267A (en) * | 2020-11-11 | 2021-03-23 | 湖南怡田美农业科技有限公司 | Cleaning structure of analyzer |
| CN112881089A (en) * | 2019-11-29 | 2021-06-01 | 深圳市帝迈生物技术有限公司 | Sampling device, liquid path system and method thereof and sample analyzer |
| CN113109583A (en) * | 2021-04-13 | 2021-07-13 | 亚能生物技术（深圳）有限公司 | Sample test tube processing device of flow cytometer |
| CN114062661A (en) * | 2020-07-30 | 2022-02-18 | 深圳市帝迈生物技术有限公司 | Sample analyzer, sampling needle cleaning liquid path and cleaning method thereof |
| CN114160529A (en) * | 2021-10-29 | 2022-03-11 | 常州必达科生物科技有限公司 | Flow-type pool cleaning system and method |
| CN114632758A (en) * | 2020-12-16 | 2022-06-17 | 深圳市帝迈生物技术有限公司 | Cleaning device, liquid path cleaning method and sample analyzer |
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| CN114160529A (en) * | 2021-10-29 | 2022-03-11 | 常州必达科生物科技有限公司 | Flow-type pool cleaning system and method |
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Application publication date: 20190521 |
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