CN104289466A - Cell analyzer and cleaning system thereof - Google Patents
Cell analyzer and cleaning system thereof Download PDFInfo
- Publication number
- CN104289466A CN104289466A CN201310298448.1A CN201310298448A CN104289466A CN 104289466 A CN104289466 A CN 104289466A CN 201310298448 A CN201310298448 A CN 201310298448A CN 104289466 A CN104289466 A CN 104289466A
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- bay
- cleaning
- afterbay
- control valve
- cleaning part
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- 238000004140 cleaning Methods 0.000 title claims abstract description 116
- 239000012459 cleaning agent Substances 0.000 claims abstract description 40
- 238000010926 purge Methods 0.000 claims description 28
- 239000003599 detergent Substances 0.000 claims description 22
- 238000005070 sampling Methods 0.000 claims description 22
- 238000001514 detection method Methods 0.000 claims description 20
- 230000003287 optical effect Effects 0.000 claims description 20
- 238000009826 distribution Methods 0.000 claims description 18
- 238000010790 dilution Methods 0.000 abstract description 14
- 239000012895 dilution Substances 0.000 abstract description 14
- 239000012530 fluid Substances 0.000 abstract description 8
- 210000004027 cell Anatomy 0.000 description 6
- 238000011109 contamination Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Cleaning In General (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
A cleaning system for a cell analyzer comprises a fluid power source, an afterbay cleaning portion and a functional unit cleaning portion. The afterbay cleaning portion is provided with an afterbay inlet and an afterbay outlet. The fluid power source is communicated to the afterbay inlet through an afterbay cleaning pipeline. The afterbay cleaning pipeline is provided with a first control valve. The afterbay outlet is connected with a discharge pipeline. The discharge pipeline is provided with a second control valve. The functional unit cleaning portion is connected to the afterbay cleaning pipeline and/or discharge pipeline. Because of the features of extremely low cell content and large dilution ratio in the afterbay, the afterbay of a counting chamber can be taken as the cleaning node. The functional unit cleaning portion of the cell analyzer is connected with the afterbay cleaning portion through a pipeline. In a cleaning step, various functional units including the afterbay can be cleaned simultaneously by a cleaning agent without carryover, so that the cleaning efficiency and the cleaning agent utilization are improved. Meanwhile, since a high pressure is needed to clean a gem hole of the afterbay, the functional units of the analyzer having a pressure difference themselves are communicated to the afterbay so as to realize simultaneous cleaning of the functional units and the gem hole.
Description
Technical field
The present invention relates to medical inspection technical field, particularly relate to a kind of cytoanalyze and purging system thereof.
Background technology
Cytoanalyze is provided with counting chamber, and counting chamber generally comprises forebay and after-bay, is communicated with between forebay with after-bay by gem hole (also known as micropore).In the cell analysis course of work, after-bay can contact with tested sample, needs to clean after-bay with the such as cleaning agent such as dilution after each measurement.
Meanwhile, cytoanalyze generally also includes functional unit as multiple in optical detection apparatus, sampling needle etc.Optical detection apparatus is the vitals carrying out leukocyte differential count, and sampling needle is used for collecting sample, all needs and sample contact, therefore also must clean with dilution after analysis to measure in cell analysis process.
So purging system is the requisite parts of cytoanalyze, primarily of the pipeline composition of transport dilution, it is communicated with fluid motion-promotion force source, and purging system drives diluent flow that dilution is delivered to parts to be cleaned by power source and cleans.But, its each functional unit of traditional cytoanalyze due to cleaning requirement different, so purging system is separate, dilution transport network complex structure, cause apparatus structure complicated, instrument cost is high, cleaning efficiency and utilization rate low.
Summary of the invention
Based on this, be necessary, for the low problem of cleaning agent transport network cleaning utilization rate, to provide a kind of purging system of cytoanalyze.
A purging system for cytoanalyze, comprising:
After-bay cleaning part, described after-bay cleaning part is provided with after-bay entrance and after-bay outlet;
Hydrodynamic source, described hydrodynamic source is communicated with described after-bay entrance by after-bay detergent line, and described after-bay detergent line is provided with the first control valve;
Discharge line, be connected to the outlet of described after-bay and discharge after-bay for cleaning agent, described discharge line is provided with the second control valve; And
Functional unit cleaning part, described functional unit cleaning part is connected to described after-bay detergent line and/or discharge line.
Wherein in an embodiment, described functional unit cleaning part is series between the outlet of described after-bay and the second control valve.
Wherein in an embodiment, described discharge line is provided with the 3rd control valve, and described 3rd control valve is arranged between the outlet of described after-bay and described functional unit cleaning part.
Wherein in an embodiment, functional unit cleaning part comprises sampling needle cleaning part, and described sampling needle cleaning part comprises the swab of cleaning sampling pin, and described swab is series between described 3rd control valve and the second control valve.
Wherein in an embodiment, described after-bay detergent line is provided with distribution pipeline, and described distribution pipeline is arranged between described first control valve and described after-bay entrance;
Institute's functional unit cleaning part comprises optical detection apparatus cleaning part, and described optical detection apparatus cleaning part is connected to described distribution pipeline.
Wherein in an embodiment, described distribution pipeline is provided with the 4th control valve.
Wherein in an embodiment, described purging system also comprises negative pressure source, and described negative pressure source is connected to described discharge line.
Wherein in an embodiment, described after-bay detergent line is provided with the 5th control valve, and described 5th control valve is arranged between described distribution pipeline and after-bay entrance.
Wherein in an embodiment, described after-bay entrance is just to the gem hole of cytoanalyze.
A kind of cytoanalyze, is characterized in that, comprise purging system as above.
The purging system of above-mentioned cytoanalyze, because the gem hole cleaning of after-bay needs larger pressure, and inner cell content is extremely low, the feature that thinner ratio is large, so counting chamber after-bay can be utilized as cleaning node, is connected the functional unit cleaning part of cytoanalyze with after-bay cleaning part by pipeline, in a cleaning link, cleaning agent can clean the multiple functional units comprising after-bay simultaneously, can not cause Residual contamination, improves cleaning efficiency and cleaning agent utilization rate.
Accompanying drawing explanation
Fig. 1 is the structural representation of purging system one embodiment of this cytoanalyze;
Fig. 2 is the structural representation of another embodiment of purging system of this cytoanalyze.
Detailed description of the invention
As shown in Figure 1, a kind of purging system of cytoanalyze, comprises after-bay cleaning part, hydrodynamic source 310 and at least one functional unit cleaning part.
Described after-bay cleaning part is provided with after-bay entrance 2111 and after-bay outlet 2112.Described after-bay entrance 2111 enters after-bay 211 for the cleaning agent as dilution etc. and cleans; Described after-bay outlet 2112 flows out after-bay 211 for the cleaning agent after cleaning after-bay 211.
Described hydrodynamic source 310 is communicated with described after-bay entrance 2111 by after-bay detergent line 111, and described after-bay detergent line 111 is provided with the first control valve 121.Described hydrodynamic source 310, for providing the mobilization dynamic of cleaning agent, can adopt syringe etc.Because cytoanalyze self is provided with as fluid power components such as syringes more, so described hydrodynamic source 310 also can share the syringe etc. of cytoanalyze.Described first control valve 121 opens and closes for the feed flow controlling hydrodynamic source 310.
Described after-bay outlet 2112 is connected with discharge line 112, and discharge after-bay 211 for cleaning agent, described discharge line 112 is provided with the second control valve 122.
Described functional unit cleaning part is connected to described after-bay detergent line 111 and/or discharge line 112.That is to say, one or more functional unit cleaning part can be provided with, be connected to after-bay detergent line 111, or be connected to discharge line 112; Or also pond detergent line 111 and discharge line 112 can all be connected with described functional unit cleaning part in the rear, with after-bay cleaning part common pall.
Wherein in an embodiment, can also include negative pressure source 320, described negative pressure source 320 is connected to described discharge line 112, is communicated to described after-bay outlet 2112 by discharge line 112.Described negative pressure source 320, for after completing cleaning, provides cleaning agent to discharge the discharge opeing power of cytoanalyze.
When the purging system of above-mentioned cytoanalyze carries out cleaning, described first control valve 121 is opened, cleaning agent as dilution etc. provides power by hydrodynamic source 310 and enters after-bay detergent line 111, clean the multiple functional units comprising after-bay 211 through after-bay cleaning part and functional unit cleaning part, cleaning agent is discharged from described discharge line 112 afterwards.Such as, when described hydrodynamic source 310 adopts syringe, dilution entrance is connected by described first control valve 121, by in dilution inhalation syringe syringe, switched by first control valve 121 and connect after-bay detergent line 111, the dilution in syringe is pushed into after-bay 211 to clean after-bay 211.
After completing cleaning, described first control valve 121 can be closed, stop supply cleaning agent.Cleaning agent in pipeline, by all discharging cell cleaning apparatus through described discharge line 112 under the suction function of described negative pressure source 320, terminates cleaning.
Described first control valve 121, except control opens and closes, can also be used for the flow controlling cleaning agent.
Larger pressure is needed because the gem hole 213 between the forebay 212 of cytoanalyze counting chamber and after-bay 211 cleans, and it is extremely low to clean the cell concentration contained in the cleaning fluid after after-bay 211, so counting chamber after-bay 211 can be utilized as cleaning node, the functional unit cleaning part of cytoanalyze is connected with after-bay cleaning part by pipeline.The functional unit itself with pressure differential is communicated with after-bay detergent line 111, and sharing described hydrodynamic source 310 provides pressure to clean; And/or utilize the cleaning agent after cleaning after-bay 211 to clean other functional unit, extremely low owing to completing the cleaning agent cell content after cleaning after-bay 211, so utilize the cleaning agent after cleaning after-bay 211 to clean other functional unit, Residual contamination problem can not be caused.So, in a cleaning link, cleaning agent cleans the multiple functional units comprising after-bay 211 simultaneously, both meets cleaning requirement, and in turn simplify purging system, and then simplifies apparatus structure, lowering apparatus cost.
In one embodiment, described functional unit cleaning part is series between described after-bay outlet 2112 and the second control valve 122.So, then the cleaning agent cleaning after-bay 211 flows out into described functional unit cleaning part from described after-bay outlet 2112, cleans connected functional unit.Complete the cleaning agent waste liquid after cleaning to discharge under negative pressure source 320 acts on from described second control valve 122.Certainly, in other embodiments, can also be, from the cleaning agent that described after-bay outlet 2112 is discharged, multiple functional unit is cleaned by pipeline flow dividing simultaneously.
Wherein in an embodiment, described discharge line 112 is provided with the 3rd control valve 123, and described 3rd control valve 123 is arranged between described after-bay outlet 2112 and described functional unit cleaning part.3rd control valve 123 opens and closes and flow for the pipeline of control flow check through being connected to the functional unit cleaning part of described discharge line 112.
Refer to Fig. 1, in a specific embodiment, described functional unit cleaning part comprises sampling needle cleaning part, and described sampling needle cleaning part comprises the swab 221 of cleaning sampling pin 222, and described swab 221 is series between described 3rd control valve 123 and the second control valve 122.Also that is to say, in the present embodiment, is the cleaning successively to after-bay 211 and these two functional units of sampling needle 222.During cleaning, cleaning agent is introduced into after-bay 211 and cleans, then flows into swab 221, cleans sampling needle 222 outer wall and swab inside.After completing cleaning, cleaning fluid is siphoned away discharge from described discharge line 112 by negative pressure source 320.Sample in counting chamber forebay 212 itself is through dilution, and sample enters its thinner ratio after after-bay 211 through gem hole 213 again and improves further.And sampling needle 222 is for collecting sample, directly and sample contact, sample content is very high.After cleaning agent cleaning after-bay 211 when described after-bay outlet 2112 is discharged, its sample content can be ignored relative to the sample content of sampling needle 222, so clean swab 221 and sampling needle 222 outer wall again, can not cause Residual contamination.In the present embodiment, the cleaning process of after-bay 211 and swab 221 is completed by an action, achieves dilution secondary and utilizes, both improve cleaning efficiency, and saved time and simplified mechanism.
Refer to Fig. 2, in another specific embodiment, described after-bay detergent line 111 is also provided with distribution pipeline 113, and described distribution pipeline 113 is arranged between described first control valve 121 and described after-bay entrance 2111.Institute's functional unit cleaning part also comprises optical detection apparatus cleaning part, and described optical detection apparatus cleaning part is connected to described distribution pipeline 113.In the present embodiment, open described first control valve 121, cleaning agent enters after-bay detergent line 111, and enters after-bay 211 through described after-bay entrance 2111 and clean; Meanwhile, cleaning agent enters described optical detection apparatus cleaning part through distribution pipeline 113, cleans optical detection apparatus 230.Through described after-bay outlet 2112, the cleaning agent after cleaning after-bay 211 is entered described sampling needle cleaning part, again for cleaning sampling needle 222.Further increase cleaning efficiency and cleaning agent utilization rate.
Owing to there is one section of elongated detecting unit (i.e. flow chamber) in optical detection apparatus 230 inside, when cleaning agent flows through this unit, larger fluid pressure can be produced.So, pressurized effect when cleaning agent enters distribution pipeline 113 is passed to described after-bay entrance 2111, cleaning agent can be made to spray from after-bay entrance 2111 and carry out back flush to gem hole 213, when not increasing pressing mechanism, utilize the pressure that cleaning fluid produces through optical detection unit, improve the cleaning efficiency of gem hole.
Wherein in an embodiment, described distribution pipeline 113 is provided with the 4th control valve 124.So, when not needing to clean optical detection apparatus 230, also can close described 4th control valve 124, cleaning agent only enters after-bay 211 and cleans.
In like manner, wherein in an embodiment, described after-bay detergent line 111 can also be provided with the 5th control valve 125, and described 5th control valve 125 is arranged between described distribution pipeline 113 and after-bay entrance 2111.When not needing to clean after-bay 211, also can close described 5th control valve 125, cleaning agent only enters described optical detection apparatus cleaning part through described distribution pipeline 113 and cleans.
Wherein in an embodiment, described after-bay entrance 2111 is just to the gem hole 213 of cytoanalyze.Cleaning agent, after described after-bay entrance 2111 sprays, directly can impact described gem hole 213, improves the developing result of rinsing gem hole 213.
In embodiment as shown in Figure 2, that the cleaning of optical detection apparatus 230, gem hole 213, after-bay 211, swab 221 and sampling needle 222 outer wall is completed in a stream, improve utilization rate and the cleaning efficiency of dilution, greatly simplify apparatus structure again, be very beneficial for the miniaturization of instrument.
Should be understood that, in other embodiments, functional unit cleaning part can be also optical detection apparatus cleaning part, and does not comprise sampling needle cleaning part.After that is to say that cleaning agent rinses optical detection apparatus 230 and after-bay 211 and gem hole 213 respectively, namely discharge cytoanalyze and also can.
In above-described embodiment, described functional unit cleaning part is illustrated for sampling needle cleaning part, optical detection apparatus cleaning part, be appreciated that, sampling needle 222 and optical detection apparatus 230 can be substituted by other functional units, accordingly, sampling needle cleaning part, optical detection apparatus cleaning part etc. also can be substituted by other functional unit cleaning part.A kind of cytoanalyze comprising purging system as above is also provided simultaneously, utilize after-bay cleaning part as cleaning node, share detergent line, in a cleaning link, cleaning agent cleans the multiple functional units comprising after-bay 211, improves cleaning efficiency and cleaning agent utilization rate, simplifies purging system, and then simplification apparatus structure, lowering apparatus cost.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. a purging system for cytoanalyze, is characterized in that, comprising:
After-bay cleaning part, described after-bay cleaning part is provided with after-bay entrance and after-bay outlet;
Hydrodynamic source, described hydrodynamic source is communicated with described after-bay entrance by after-bay detergent line, and described after-bay detergent line is provided with the first control valve;
Discharge line, be connected to the outlet of described after-bay and discharge after-bay for cleaning agent, described discharge line is provided with the second control valve; And
Functional unit cleaning part, described functional unit cleaning part is connected to described after-bay detergent line and/or discharge line.
2. the purging system of cytoanalyze according to claim 1, is characterized in that: described functional unit cleaning part is series between the outlet of described after-bay and the second control valve.
3. the purging system of cytoanalyze according to claim 2, is characterized in that: described discharge line is provided with the 3rd control valve, and described 3rd control valve is arranged between the outlet of described after-bay and described functional unit cleaning part.
4. the purging system of cytoanalyze according to claim 3, is characterized in that: functional unit cleaning part comprises sampling needle cleaning part, and described sampling needle cleaning part comprises swab, and described swab is series between described 3rd control valve and the second control valve.
5. the purging system of the cytoanalyze according to Claims 1 to 4 any one, is characterized in that, described purging system also comprises negative pressure source, and described negative pressure source is connected to described discharge line.
6. the purging system of the cytoanalyze according to Claims 1 to 4 any one, is characterized in that,
Described after-bay detergent line is provided with distribution pipeline, and described distribution pipeline is arranged between described first control valve and described after-bay entrance;
Institute's functional unit cleaning part comprises optical detection apparatus cleaning part, and described optical detection apparatus cleaning part is connected to described distribution pipeline.
7. the purging system of cytoanalyze according to claim 6, is characterized in that, described distribution pipeline is provided with the 4th control valve.
8. the purging system of cytoanalyze according to claim 6, is characterized in that, described after-bay detergent line is provided with the 5th control valve, and described 5th control valve is arranged between described distribution pipeline and after-bay entrance.
9. the purging system of cytoanalyze according to claim 6, is characterized in that, described after-bay entrance is just to the gem hole of cytoanalyze.
10. a cytoanalyze, is characterized in that, comprises purging system as claimed in any one of claims 1 to 9 wherein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310298448.1A CN104289466B (en) | 2013-07-16 | 2013-07-16 | Cytoanalyze and its purging system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310298448.1A CN104289466B (en) | 2013-07-16 | 2013-07-16 | Cytoanalyze and its purging system |
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| CN104289466A true CN104289466A (en) | 2015-01-21 |
| CN104289466B CN104289466B (en) | 2017-03-15 |
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| CN201310298448.1A Active CN104289466B (en) | 2013-07-16 | 2013-07-16 | Cytoanalyze and its purging system |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105880204A (en) * | 2016-05-06 | 2016-08-24 | 南京医科大学 | Fast cleaning method for gold-bearing matrix detection plate for cell analyzer |
| CN107290556A (en) * | 2016-03-31 | 2017-10-24 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of sample analyser |
| CN109444028A (en) * | 2018-12-26 | 2019-03-08 | 常州必达科生物科技有限公司 | A kind of particle analyzer and its liquid channel system, discharging of waste liquid method |
| WO2019061503A1 (en) * | 2017-09-30 | 2019-04-04 | 深圳迈瑞生物医疗电子股份有限公司 | Sample analyzer cleaning control method and sample analyzer |
| CN109772822A (en) * | 2018-12-31 | 2019-05-21 | 苏州朗如精密机械科技有限公司 | A kind of stream type cell analyzer automatic washing system and its working method |
| CN111893032A (en) * | 2019-12-12 | 2020-11-06 | 山东鑫科生物科技股份有限公司 | Bacteria counting device with air cleaning mechanism |
| CN112536267A (en) * | 2020-11-11 | 2021-03-23 | 湖南怡田美农业科技有限公司 | Cleaning structure of analyzer |
| CN113926775A (en) * | 2021-10-15 | 2022-01-14 | 昆明医科大学第一附属医院 | Cleaning device and cleaning method for nozzle system of flow cytometry |
| CN114632758A (en) * | 2020-12-16 | 2022-06-17 | 深圳市帝迈生物技术有限公司 | Cleaning device, liquid path cleaning method and sample analyzer |
| CN115463926A (en) * | 2022-10-22 | 2022-12-13 | 中山市创艺生化工程有限公司 | Throttling pipeline for blood globe |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107290556A (en) * | 2016-03-31 | 2017-10-24 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of sample analyser |
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| CN112536267A (en) * | 2020-11-11 | 2021-03-23 | 湖南怡田美农业科技有限公司 | Cleaning structure of analyzer |
| CN114632758A (en) * | 2020-12-16 | 2022-06-17 | 深圳市帝迈生物技术有限公司 | Cleaning device, liquid path cleaning method and sample analyzer |
| CN114632758B (en) * | 2020-12-16 | 2024-04-09 | 深圳市帝迈生物技术有限公司 | Cleaning device, liquid path cleaning method and sample analyzer |
| CN113926775A (en) * | 2021-10-15 | 2022-01-14 | 昆明医科大学第一附属医院 | Cleaning device and cleaning method for nozzle system of flow cytometry |
| CN115463926A (en) * | 2022-10-22 | 2022-12-13 | 中山市创艺生化工程有限公司 | Throttling pipeline for blood globe |
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| CN104289466B (en) | 2017-03-15 |
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