CN113926775A - Cleaning device and cleaning method for nozzle system of flow cytometry - Google Patents
Cleaning device and cleaning method for nozzle system of flow cytometry Download PDFInfo
- Publication number
- CN113926775A CN113926775A CN202111202329.2A CN202111202329A CN113926775A CN 113926775 A CN113926775 A CN 113926775A CN 202111202329 A CN202111202329 A CN 202111202329A CN 113926775 A CN113926775 A CN 113926775A
- Authority
- CN
- China
- Prior art keywords
- cleaning
- pipe
- tube
- wall
- flow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
- B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
- B08B9/0321—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing using pressurised, pulsating or purging fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/0804—Cleaning containers having tubular shape, e.g. casks, barrels, drums
- B08B9/0813—Cleaning containers having tubular shape, e.g. casks, barrels, drums by the force of jets or sprays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
- B08B9/28—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by splash, spray, or jet application, with or without soaking
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a cleaning device and a cleaning method for a nozzle system of a flow cytometry, which comprises a flow cytometry shell, a cleaning box and a sample tube, wherein the side wall of the sample tube is communicated with a threaded tube; a transmission pipe is arranged in the cleaning box, one end of the transmission pipe penetrates through the cleaning box and is connected with an installation part, the installation part is used for communicating the transmission pipe and a threaded pipe, a temperature control part and a flow detection part are arranged on the side wall of the transmission pipe, and a cleaning agent self-switching part and an air source part are arranged in the cleaning box; the side wall of the cleaning box is provided with a cleaning agent circulating device, and the cleaning agent circulating device comprises a shunting part arranged at the liquid discharge end of the shell of the flow cytometry, a water quality detection part arranged on the outer wall of the cleaning box and connected with the shunting part through a circulating pipe, and a circulating part arranged on the inner wall of the cleaning box and used for circularly discharging liquid in the water quality detection part into a transmission pipe. The invention provides a cleaning device and a cleaning method which are convenient for cleaning a sample tube and an analyzer nozzle system together.
Description
Technical Field
The invention mainly relates to the technical field of cleaning of medical equipment, in particular to a cleaning device and a cleaning method for a nozzle system of a flow cytometry.
Background
Flow cytometers can rapidly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in a liquid, and can sort out specified cell subsets according to preselected parameter ranges.
According to a full-automatic cleaning system for a flow cytometer and an operating method thereof provided by the patent document with the application number of CN201811651055.3, the system comprises a washing liquid tank, a first cleaning pipeline, a first electromagnetic valve, a first cleaning branch, a second electromagnetic valve, a front pool, a rear pool, a gem hole, a first liquid inlet pipe, a first liquid outlet pipe, a third electromagnetic valve, a sampling needle, a swab, a fourth electromagnetic valve, a second cleaning branch, a third cleaning branch, a group of reaction cup cleaning needles, a group of liquid suction needles, a second liquid discharge pipe and a waste liquid discharge port. The system can clean each unit of the cell analyzer, improves the use efficiency of the cleaning agent, can ensure the cleaning effect of the cell analyzer, and is safe and reliable to use.
The product in the above-mentioned patent can carry out the washing of each unit of cell analysis appearance to the availability factor of cleaner has been improved, nevertheless is not convenient for wash sample tube and analysis appearance nozzle system together, is not convenient for control the washing liquid temperature velocity of flow, and is not convenient for wash and carries out the air-drying after accomplishing and handle.
Disclosure of Invention
The invention mainly provides a cleaning device and a cleaning method for a nozzle system of a flow cytometry, which are used for solving the technical problems in the background technology.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a cleaning device of a nozzle system of a flow cytometer comprises a flow cytometer shell, a cleaning box and a sample tube, wherein the side wall of the sample tube is communicated with a threaded tube, and a rubber ring is arranged on the inner wall of the threaded tube;
a conveying pipe is arranged in the cleaning box, one end of the conveying pipe penetrates through the cleaning box and is connected with an installation part, the installation part is used for communicating the conveying pipe and a threaded pipe, a temperature control part and a flow detection part are arranged on the side wall of the conveying pipe and positioned in the cleaning box, a cleaning agent self-switching part and an air source part are arranged in the cleaning box, and execution ends of the cleaning agent self-switching part and the air source part are communicated with the conveying pipe;
wash the case lateral wall and be equipped with cleaner circulating device, cleaner circulating device is including locating the reposition of redundant personnel part of flow cytometry shell flowing back end locates wash the case outer wall and through the water quality testing part that circulating pipe and reposition of redundant personnel part are connected, and locate wash incasement wall and be used for discharging the circulation part of transmission pipe into water quality testing part inner liquid circulation.
Preferably, the mounting component comprises a communicating pipe arranged at one end of the transmission pipe and a locking pipe rotatably connected with the outer wall of the communicating pipe, the communicating pipe penetrates through the rubber ring and extends into the sample pipe, and the inner wall of the locking pipe is in threaded connection with the outer wall of the threaded pipe. In the preferred embodiment, the mounting member facilitates stable communication of the transfer tube with the sample tube.
Preferably, the temperature control component comprises a temperature sensor arranged on the inner wall of the transmission pipe, a positioning pipe sleeved on the outer wall of the transmission pipe, and a spiral heating pipe sleeved on the outer wall of the transmission pipe and positioned inside the positioning pipe. In the preferred embodiment, the cleaning temperature is conveniently controlled by the temperature control component.
Preferably, the flow detection component comprises a flow sensor arranged on the outer wall of the transmission pipe. In the preferred embodiment, the flow detection component is used for conveniently controlling the flow rate, so that the flow cell analyzer shell internal pipeline is prevented from being damaged due to the too fast flow rate.
Preferably, the cleaning agent is from switching part including locating wash a plurality of liquid storage tanks of incasement wall bottom, the intercommunication the liquid storage tank top just extends to the outside pneumatic tube of washing case, locates the first automatically controlled valve of liquid storage tank flowing back end to and communicate a plurality ofly the inlet pipe of first automatically controlled valve flowing back end, the inlet pipe passes through pipeline intercommunication transmission pipe. In the preferred embodiment, the cleaning agent self-switching component is used for facilitating the introduction of different cleaning agents into the conveying pipe.
Preferably, the air source component comprises an air storage tank arranged at the bottom of the inner wall of the cleaning box, an air source pipe communicated with the top of the air storage tank and extending to the outside of the cleaning box, and a second electric control valve arranged at the liquid discharge end of the air storage tank, wherein the liquid discharge end of the second electric control valve is communicated with the transmission pipe through a pipeline. In the preferred embodiment, the supply of the drying gas into the transfer line is facilitated by a gas supply means to facilitate drying of the line.
Preferably, the reposition of redundant personnel part is including locating the shunt tubes of flow cytometry shell flowing back end, shunt tubes outer wall intercommunication blow off pipe and circulating pipe, the shunt tubes is kept away from the one end of flow cytometry shell is equipped with the driving cylinder, the driving cylinder execution end runs through the shunt tubes and connects the plunger plate, the plunger plate lateral wall is equipped with the closing plate, be equipped with the through-hole on the closing plate, the through-hole with the circulating pipe position corresponds. In the preferred embodiment, the shunt member facilitates circulation of the cleaning agent during cleaning, and allows normal drainage of the effluent during use of the flow cytometer housing.
Preferably, the water quality detection part comprises a water quality detection box arranged on the outer wall of the cleaning box, a groove arranged at the bottom of the inner wall of the water quality detection box, a water quality sensor arranged at the bottom of the groove, and a drainage pipe communicated with the side wall of the water quality detection box and positioned at the bottom of the side wall of the groove, wherein a third electric control valve is arranged on the drainage pipe, and the side wall of the water quality detection box is communicated with a circulating pipe. In the preferred embodiment, the water quality detection part is used for conveniently detecting the cleaning agent and discharging the circulating liquid when the pollutant is larger than a set value.
Preferably, the circulating part comprises a circulating pump arranged on the inner wall of the cleaning box, and the liquid inlet end of the circulating pump is communicated with the water quality detection box through a pipeline, and the liquid discharge end of the circulating pump is communicated with the transmission pipe through a pipeline. In the preferred embodiment, circulation of the cleaning agent is facilitated by a circulation member.
According to the technical scheme of the cleaning device of the nozzle system of the flow cytometer, a cleaning method of the cleaning device of the nozzle system of the flow cytometer is further provided, and the cleaning method comprises the following steps:
the method comprises the following steps that firstly, devices are connected, a transmission pipe is communicated with a sample pipe through a mounting component, and the sample pipe is placed at a sampling position of a shell of the flow cytometry analyzer;
step two, circulating cleaning, namely introducing a certain amount of cleaning agent into the transmission pipe through the cleaning agent self-switching part, wherein the cleaning agent sequentially passes through the transmission pipe, the sample pipe, the flow cytometry shell, the shunt part, the water quality detection part and the circulating part to form circulation so as to circularly clean the pipeline inside the shell of the flow cytometry, the cleaning agent is discharged after being circularly cleaned for unit turns, the cleaning agent is switched from the self-switching part to output deionized water, the deionized water is circularly washed for the pipeline inside the shell of the flow cytometry, and the deionized water is discharged after being circularly cleaned for unit turns;
during cleaning, the temperature of the liquid is controlled by the temperature control part, the flow rate of the liquid is controlled by the flow detection part, the liquid is detected by the water quality detection part, and the circulating liquid is discharged when the pollutants are larger than a set value so as to be replaced by new circulating liquid;
and step three, drying the air flow, namely introducing a drying air flow into the cleaned pipeline through an air source part so as to air-dry the interior of the pipeline.
Compared with the prior art, the invention has the beneficial effects that:
the cleaning device is convenient for cleaning the sample tube and the analyzer nozzle system together, is convenient for controlling the temperature and the flow rate of the cleaning solution, and is convenient for air drying treatment after cleaning;
be convenient for through the installation part with the transmission pipe with the sample pipe stable intercommunication, be convenient for let in different cleaner to the transmission pipe through cleaner self-switching part, be convenient for let in dry gas to the transmission pipe through the air supply part, so that the drying of pipeline, be convenient for carry out the circulation of cleaner when wasing through the reposition of redundant personnel part, normal blowdown when flow cell analyzer shell uses, be convenient for detect the cleaner and discharge the circulating fluid when the pollutant is greater than the setting value through the water quality testing part, be convenient for circulate the cleaner through the circulating part, be convenient for control the cleaning temperature through the accuse temperature part, be convenient for control the velocity of flow through the flow detection part, prevent that the velocity of flow from producing the damage to flow cell analyzer shell inner pipeline too fast.
The present invention will be explained in detail below with reference to the drawings and specific embodiments.
Drawings
FIG. 1 is an isometric view of the overall construction of the present invention;
FIG. 2 is an exploded view of the overall structure of the present invention;
FIG. 3 is an isometric view of the cleaning agent circulation device of the present invention;
FIG. 4 is an isometric view of the internal structure of the cleaning tank of the present invention;
FIG. 5 is a top view of the overall structure of the present invention;
FIG. 6 is a cross-sectional view of the overall construction of the present invention;
FIG. 7 is a cross-sectional view of a transfer tube configuration of the present invention;
FIG. 8 is a sectional view showing the structure of a water quality detecting member of the present invention.
Description of the drawings: 10. a flow cytometer housing; 20. a cleaning tank; 21. a conveying pipe; 22. a mounting member; 221. a communicating pipe; 222. a locking tube; 23. a temperature control component; 231. a temperature sensor; 232. a positioning tube; 233. a spiral heating pipe; 24. a flow rate detection section; 241. a flow sensor; 25. a cleaning agent self-switching member; 251. a liquid storage tank; 252. a pneumatic tube; 253. a first electrically controlled valve; 254. a feed pipe; 26. a gas source component; 261. a gas storage tank; 262. a gas source pipe; 263. a second electrically controlled valve; 30. a cleaning agent circulating device; 31. a flow dividing member; 311. a shunt tube; 312. a blow-off pipe; 313. a drive cylinder; 314. a plunger plate; 315. a sealing plate; 316. a through hole; 32. a circulation pipe; 33. a water quality detection part; 331. a water quality detection box; 332. a groove; 333. a water quality sensor; 334. a drain pipe; 335. a third electrically controlled valve; 34. a circulating member; 341. a circulation pump; 40. a sample tube; 41. a threaded pipe; 42. a rubber ring.
Detailed Description
In order to facilitate an understanding of the invention, the invention will now be described more fully hereinafter with reference to the accompanying drawings, in which several embodiments of the invention are shown, but which may be embodied in different forms and not limited to the embodiments described herein, but which are provided so as to provide a more thorough and complete disclosure of the invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may be present, and when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present, as the terms "vertical", "horizontal", "left", "right" and the like are used herein for descriptive purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and the knowledge of the terms used herein in the specification of the present invention is for the purpose of describing particular embodiments and is not intended to limit the present invention, and the term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1, 2 and 7, in a preferred embodiment of the present invention, a cleaning apparatus for a nozzle system of a flow cytometer includes a flow cytometer housing 10, a cleaning box 20 and a sample tube 40, wherein a side wall of the sample tube 40 is connected to a threaded tube 41, and a rubber ring 42 is disposed on an inner wall of the threaded tube 41; the mounting part 22 comprises a communication pipe 221 arranged at one end of the transmission pipe 21 and a locking pipe 222 rotatably connected with the outer wall of the communication pipe 221, the communication pipe 221 penetrates through the rubber ring 42 and extends into the sample pipe 40, and the inner wall of the locking pipe 222 is in threaded connection with the outer wall of the threaded pipe 41.
In the present embodiment, the transfer tube 21 is connected to the sample tube 40 via the mounting member 22, and the sample tube 40 is placed at the sampling position of the flow cytometer housing 10, that is, the sample tube 40 is fitted to the outside of the nozzle of the flow cytometer housing 10;
further, when the mounting member 22 is used, the connection pipe 221 is inserted into the sample tube 40 through the rubber ring 42, and the lock tube 222 is rotated to screw the inner wall of the lock tube 222 to the outer wall of the screw tube 41.
Please refer to fig. 2, 3, 4, 6, and 7, in another preferred embodiment of the present invention, a transmission pipe 21 is disposed in the cleaning tank 20, one end of the transmission pipe 21 penetrates through the cleaning tank 20 and is connected to an installation component 22, the installation component 22 is used for communicating the transmission pipe 21 with a threaded pipe 41, a temperature control component 23 and a flow detection component 24 are disposed on a side wall of the transmission pipe 21 and located in the cleaning tank 20, a cleaning agent self-switching component 25 and an air source component 26 are disposed in the cleaning tank 20, and execution ends of the cleaning agent self-switching component 25 and the air source component 26 are both communicated with the transmission pipe 21; the temperature control component 23 comprises a temperature sensor 231 arranged on the inner wall of the conveying pipe 21, a positioning pipe 232 sleeved on the outer wall of the conveying pipe 21, and a spiral heating pipe 233 sleeved on the outer wall of the conveying pipe 21 and located inside the positioning pipe 232, the flow detection component 24 comprises a flow sensor 241 arranged on the outer wall of the conveying pipe 21, the cleaning agent self-switching component 25 comprises a plurality of liquid storage tanks 251 arranged at the bottom of the inner wall of the cleaning box 20, an air pressure pipe 252 communicated with the top of the liquid storage tanks 251 and extending to the outside of the cleaning box 20, first electric control valves 253 arranged at the liquid discharge ends of the liquid storage tanks 251, and a feeding pipe 254 communicated with the liquid discharge ends of the first electric control valves 253, the feeding pipe 254 is communicated with the conveying pipe 21 through a pipeline, the air source component 26 comprises an air storage tank 261 arranged at the bottom of the inner wall of the cleaning box 20, and an air source pipe 262 communicated with the top of the air storage tank 261 and extending to the outside of the cleaning box 20, and a second electric control valve 263 arranged at the liquid discharge end of the air storage tank 261, wherein the liquid discharge end of the second electric control valve 263 is communicated with the transmission pipe 21 through a pipeline.
It should be noted that, in this embodiment, a certain amount of cleaning agent, such as a flow cytometer cleaning solution, is introduced into the transfer tube 21 through the cleaning agent self-switching component 25, the cleaning agent sequentially passes through the transfer tube 21, the sample tube 40, the flow cytometer housing 10, the shunt component 31, the water quality detection component 33, and the circulation component 34 to form a circulation, so as to perform a circulation cleaning on the internal pipeline of the flow cytometer housing 10, the cleaning agent is discharged after being circularly cleaned for a unit number of turns, the cleaning agent is switched from the switching component 25 to output another cleaning agent, such as deionized water, the deionized water performs a circulation cleaning on the internal pipeline of the flow cytometer housing 10, the cleaning agent is discharged after being circularly for a unit number of turns, and after the cleaning is completed, a dry airflow is introduced into the cleaned pipeline through the air source component 26, so as to air-dry the interior of the pipeline;
during cleaning, the temperature of the liquid is controlled by the temperature control part 23, and the flow rate of the liquid is controlled by the flow detection part 24;
further, when the cleaning agent self-switching part 25 works, the first electronic control valve 253 corresponding to the liquid storage tank 251 for storing the flow cytometer cleaning solution inside is opened, at this time, the flow cytometer cleaning solution enters the transmission pipe 21 through the feeding pipe 254, when the cleaning agent is switched, the first electronic control valve 253 corresponding to the liquid storage tank 251 for storing the flow cytometer cleaning solution inside is closed, the first electronic control valve 253 corresponding to the liquid storage tank 251 for storing the deionized water inside is opened, the switching can be completed, and the air pressure pipe 252 is convenient for inputting pressure air into the liquid storage tank 251 so as to facilitate the feeding of the cleaning agent;
further, when the air source component 26 is in operation, the air source pipe 262 can input dry air into the air storage tank 261, and the second electronic control valve 263 is opened, so that the dry air enters the conveying pipe 21;
further, when the temperature control component 23 works, the PLC controller receives the temperature data of the temperature sensor 231 and triggers the spiral heating pipe 233 until the temperature data is consistent with a set value;
further, when the flow rate detecting unit 24 is operated, the PLC controller receives the flow rate data of the flow rate sensor 241 and changes the power of the circulation pump 341 until the flow rate data is consistent with the set value.
Please refer to fig. 2, 5, 6, and 8, in another preferred embodiment of the present invention, a cleaning agent circulation device 30 is disposed on a sidewall of the cleaning tank 20, the cleaning agent circulation device 30 includes a flow dividing component 31 disposed at a drainage end of the flow cytometer housing 10, a water quality detecting component 33 disposed on an outer wall of the cleaning tank 20 and connected to the flow dividing component 31 via a circulation pipe 32, and a circulation component 34 disposed on an inner wall of the cleaning tank 20 and used for circulating the liquid in the water quality detecting component 33 into the transmission pipe 21, the flow dividing component 31 includes a flow dividing pipe 311 disposed at the drainage end of the flow cytometer housing 10, an outer wall of the flow dividing pipe 311 is connected to the drainage pipe 312 and the circulation pipe 32, a driving cylinder 313 is disposed at an end of the flow dividing pipe 311 away from the flow cytometer housing 10, an execution end of the driving cylinder 313 penetrates through the flow dividing pipe 311 to connect the plug plate 314, plunger plate 314 lateral wall is equipped with closing plate 315, be equipped with through-hole 316 on the closing plate 315, through-hole 316 with the circulating pipe 32 position corresponds, water quality testing part 33 is including locating the water quality testing case 331 who washs the outer wall of case 20 locates the recess 332 of water quality testing case 331 inner wall bottom locates the water quality sensor 333 of recess 332 bottom, and the intercommunication the lateral wall of water quality testing case 331 just is located the drain pipe 334 of recess 332 lateral wall bottom, be equipped with third automatically controlled valve 335 on the drain pipe 334, water quality testing case 331 lateral wall intercommunication circulating pipe 32, circulating part 34 is including locating the circulating pump 341 who washs the case 20 inner wall, circulating pump 341 feed liquor end passes through pipeline intercommunication water quality testing case 331, flowing back end passes through pipeline intercommunication transmission pipe 21.
It should be noted that, in this embodiment, the flow dividing component 31 facilitates circulation of the cleaning agent during cleaning, normal pollution discharge is performed when the flow cytometer housing 10 is used, and the water quality detection component 33 detects the liquid and discharges the circulating liquid when the pollutant is greater than a set value, so as to replace a new circulating liquid;
further, when the flow dividing part 31 works, when the through hole 316 coincides with the circulating pipe 32, the plunger plate 314 seals the sewage discharge pipe 312, at this time, liquid enters the circulating pipe 32 through the through hole 316, after the driving cylinder 313 drives the plunger plate 314 to move, the through hole 316 is dislocated with the circulating pipe 32, at this time, the plunger plate 314 cancels the sealing of the sewage discharge pipe 312, and the liquid is discharged through the sewage discharge pipe 312;
further, when the water quality detecting part 33 is operated, the cleaning solution discharged through the circulation pipe 32 enters the water quality detecting tank 331 and fills the groove 332, the water quality sensor 333 transmits water quality information to the PLC controller, the PLC controller analyzes the water quality information, then the circulation pump 341 is turned on when the amount of pollutants is smaller than a set value to circulate the cleaning solution, the circulation pump 341 is turned off when the amount of pollutants is larger than the set value, and the third electronic control valve 335 is turned on to discharge the cleaning solution through the drainage pipe 334.
According to the above embodiments, there will also be provided a method of cleaning a cleaning device of a flow cytometer nozzle system, comprising the steps of:
firstly, connecting devices, namely communicating a transmission tube 21 with a sample tube 40 through a mounting component 22, and placing the sample tube 40 at a sampling position of a shell 10 of the flow cytometry analyzer;
step two, circulating cleaning, namely introducing a certain amount of cleaning agent into the transmission pipe 21 through the cleaning agent self-switching part 25, forming circulation after the cleaning agent sequentially passes through the transmission pipe 21, the sample pipe 40, the flow cytometer shell 10, the shunt part 31, the water quality detection part 33 and the circulation part 34 so as to circularly clean the pipeline inside the flow cytometer shell 10, discharging the cleaning agent after circularly cleaning unit turns, switching the cleaning agent from the switching part 25 to outputting deionized water, and circularly washing the pipeline inside the flow cytometer shell 10 by the deionized water, and discharging the deionized water after circularly cleaning unit turns;
during cleaning, the temperature of the liquid is controlled by the temperature control part 23, the flow rate of the liquid is controlled by the flow detection part 24, the liquid is detected by the water quality detection part 33, and the circulating liquid is discharged when the pollutant is larger than a set value so as to be replaced by new circulating liquid;
and step three, drying the air flow, namely introducing a drying air flow into the cleaned pipeline through the air source part 26 so as to air-dry the interior of the pipeline.
The specific process of the invention is as follows:
the model of the PLC is 6ES7214-1BD23-0XB8, the model of the temperature sensor 231 is DS18B20, the model of the flow sensor 241 is CX, and the model of the water quality sensor 333 is XZ 3010.
The transmission tube 21 is communicated with the sample tube 40 through the mounting component 22, and the sample tube 40 is placed at the sampling position of the flow cytometry shell 10, namely the sample tube 40 is sleeved outside the nozzle of the flow cytometry shell 10;
when the mounting part 22 is used, the communicating pipe 221 penetrates through the rubber ring 42 and is inserted into the sample tube 40, and the locking tube 222 is rotated to connect the inner wall of the locking tube 222 with the outer wall of the threaded tube 41 in a threaded manner;
a certain amount of cleaning agent, such as a flow cytometer cleaning solution, is introduced into the transmission pipe 21 through the cleaning agent self-switching component 25, the cleaning agent sequentially passes through the transmission pipe 21, the sample pipe 40, the flow cytometer housing 10, the shunt component 31, the water quality detection component 33 and the circulation component 34 to form circulation, so that the pipeline inside the flow cytometer housing 10 is circularly cleaned, the cleaning agent is discharged after being circularly cleaned for unit turns, the cleaning agent is switched from the switching component 25 to output another cleaning agent, such as deionized water, the deionized water circularly washes the pipeline inside the flow cytometer housing 10, the cleaning agent is discharged after being circularly cleaned for unit turns, and after the cleaning is completed, dry airflow is introduced into the cleaned pipeline through the air source component 26 to air dry the inside of the pipeline;
during cleaning, the temperature of the liquid is controlled by the temperature control part 23, and the flow rate of the liquid is controlled by the flow detection part 24;
when the cleaning agent self-switching component 25 works, the first electronic control valve 253 corresponding to the liquid storage tank 251 for storing the flow cytometer cleaning solution inside is opened, at the moment, the flow cytometer cleaning solution enters the transmission pipe 21 through the feeding pipe 254, when the cleaning agent is switched, the first electronic control valve 253 corresponding to the liquid storage tank 251 for storing the flow cytometer cleaning solution inside is closed, the first electronic control valve 253 corresponding to the liquid storage tank 251 for storing deionized water inside is opened, the switching can be completed, and the air pressure pipe 252 is convenient for inputting pressure air into the liquid storage tank 251 so as to facilitate the feeding of the cleaning agent;
when the air source part 26 works, the air source pipe 262 can input dry air into the air storage tank 261, the second electric control valve 263 is opened, and the dry air enters the transmission pipe 21;
when the temperature control component 23 works, the PLC controller receives the temperature data of the temperature sensor 231 and triggers the spiral heating pipe 233 until the temperature data is consistent with a set value;
when the flow detection part 24 works, the PLC controller receives flow data of the flow sensor 241 and changes the power of the circulation pump 341 until the flow data is consistent with a set value;
the flow distribution part 31 facilitates circulation of a cleaning agent during cleaning, normal pollution discharge is performed when the flow cell analyzer housing 10 is used, the water quality detection part 33 detects liquid and discharges the circulating liquid when the pollutant is larger than a set value so as to replace new circulating liquid;
when the flow dividing part 31 works, when the through hole 316 is superposed with the circulating pipe 32, the plunger plate 314 seals the sewage discharge pipe 312, at the moment, liquid enters the circulating pipe 32 through the through hole 316, after the driving cylinder 313 drives the plunger plate 314 to move, the through hole 316 is staggered with the circulating pipe 32, at the moment, the plunger plate 314 cancels the sealing of the sewage discharge pipe 312, and the liquid is discharged through the sewage discharge pipe 312;
when the water quality detecting part 33 is operated, the cleaning liquid discharged through the circulating pipe 32 enters the water quality detecting tank 331 and fills the groove 332, the water quality sensor 333 transmits water quality information to the PLC controller, the PLC controller analyzes the water quality information, turns on the circulating pump 341 when the pollutant amount is smaller than a set value to circulate the cleaning agent, turns off the circulating pump 341 when the pollutant amount is larger than the set value, and turns on the third electrically controlled valve 335 to discharge the cleaning agent through the drain pipe 334.
The invention is described above with reference to the accompanying drawings, it is obvious that the invention is not limited to the above-described embodiments, and it is within the scope of the invention to adopt such insubstantial modifications of the inventive method concept and solution, or to apply the inventive concept and solution directly to other applications without modification.
Claims (10)
1. A cleaning device of a flow cytometry nozzle system comprises a flow cytometry shell (10), a cleaning box (20) and a sample tube (40), and is characterized in that the side wall of the sample tube (40) is communicated with a threaded tube (41), and a rubber ring (42) is arranged on the inner wall of the threaded tube (41);
a conveying pipe (21) is arranged in the cleaning box (20), one end of the conveying pipe (21) penetrates through the cleaning box (20) and is connected with a mounting component (22), the mounting component (22) is used for communicating the conveying pipe (21) with a threaded pipe (41), a temperature control component (23) and a flow detection component (24) are arranged on the side wall of the conveying pipe (21) and located in the cleaning box (20), a cleaning agent self-switching component (25) and an air source component (26) are arranged in the cleaning box (20), and execution ends of the cleaning agent self-switching component (25) and the air source component (26) are both communicated with the conveying pipe (21);
wash case (20) lateral wall and be equipped with cleaner circulating device (30), cleaner circulating device (30) are including locating flow cytometry shell (10) flowing back end reposition of redundant personnel part (31), locate wash case (20) outer wall and through circulating pipe (32) with reposition of redundant personnel part (31) be connected water quality testing part (33), and locate wash case (20) inner wall and be used for discharging into circulation part (34) of transmission pipe (21) with water quality testing part (33) interior liquid circulation.
2. The cleaning device for nozzle system of flow cytometer analyzer as described in claim 1, wherein said mounting component (22) comprises a connection tube (221) disposed at one end of said transport tube (21), and a locking tube (222) rotatably connected to an outer wall of said connection tube (221), said connection tube (221) penetrates through said rubber ring (42) and extends into said sample tube (40), and an inner wall of said locking tube (222) is screwed to an outer wall of said threaded tube (41).
3. The cleaning device of the nozzle system of the flow cytometer as described in claim 1, wherein said temperature control component (23) comprises a temperature sensor (231) disposed on the inner wall of said transport tube (21), a positioning tube (232) sleeved on the outer wall of said transport tube (21), and a spiral heating tube (233) sleeved on the outer wall of said transport tube (21) and located inside said positioning tube (232).
4. A cleaning apparatus for a nozzle system of a flow cytometer as described in claim 1, wherein said flow detection component (24) comprises a flow sensor (241) disposed on an outer wall of said transfer tube (21).
5. The cleaning device of the nozzle system of the flow cytometer as described in claim 1, wherein the cleaning agent self-switching component (25) comprises a plurality of liquid storage tanks (251) disposed at the bottom of the inner wall of the cleaning box (20), a pneumatic tube (252) communicating with the top of the liquid storage tanks (251) and extending to the outside of the cleaning box (20), a first electrically controlled valve (253) disposed at the liquid discharge end of the liquid storage tanks (251), and a feeding tube (254) communicating with the liquid discharge ends of the first electrically controlled valves (253), wherein the feeding tube (254) communicates with the transmission tube (21) through a pipeline.
6. The cleaning device of the nozzle system of the flow cytometer as described in claim 1, wherein the gas source component (26) comprises a gas storage tank (261) disposed at the bottom of the inner wall of the cleaning box (20), a gas source pipe (262) communicating with the top of the gas storage tank (261) and extending to the outside of the cleaning box (20), and a second electrically controlled valve (263) disposed at the discharge end of the gas storage tank (261), the discharge end of the second electrically controlled valve (263) being in pipeline communication with the transmission pipe (21).
7. The cleaning device for the nozzle system of the flow cytometer analyzer as described in claim 1, wherein the flow dividing component (31) comprises a flow dividing pipe (311) disposed at the drainage end of the flow cytometer housing (10), the outer wall of the flow dividing pipe (311) is connected to a drainage pipe (312) and a circulation pipe (32), a driving cylinder (313) is disposed at one end of the flow dividing pipe (311) far away from the flow cytometer housing (10), the execution end of the driving cylinder (313) penetrates through the flow dividing pipe (311) to connect the plunger plate (314), a sealing plate (315) is disposed on the sidewall of the plunger plate (314), a through hole (316) is disposed on the sealing plate (315), and the through hole (316) corresponds to the circulation pipe (32).
8. The cleaning device for the nozzle system of the flow cytometer as described in claim 1, wherein the water quality detection component (33) comprises a water quality detection box (331) disposed on the outer wall of the cleaning box (20), a groove (332) disposed at the bottom of the inner wall of the water quality detection box (331), a water quality sensor (333) disposed at the bottom of the groove (332), and a drainage pipe (334) communicating with the sidewall of the water quality detection box (331) and located at the bottom of the sidewall of the groove (332), wherein a third electrically controlled valve (335) is disposed on the drainage pipe (334), and the sidewall of the water quality detection box (331) is communicated with the circulation pipe (32).
9. The cleaning device for the nozzle system of the flow cytometer analyzer as described in claim 8, wherein said circulation component (34) comprises a circulation pump (341) disposed on the inner wall of said cleaning tank (20), the inlet end of said circulation pump (341) is connected to the water quality detection tank (331) through a pipeline, and the drain end is connected to the delivery pipe (21) through a pipeline.
10. A method of cleaning a cleaning device for a flow cytometer nozzle system, comprising the steps of:
the method comprises the following steps of firstly, connecting the device, communicating a transmission tube (21) with a sample tube (40) through a mounting part (22), and placing the sample tube (40) at a sampling position of a shell (10) of the flow cytometry;
step two, circulating cleaning, namely introducing a certain amount of cleaning agent into the transmission pipe (21) through the cleaning agent self-switching component (25), wherein the cleaning agent sequentially passes through the transmission pipe (21), the sample pipe (40), the flow cytometry shell (10), the shunt component (31), the water quality detection component (33) and the circulation component (34) to form circulation so as to circularly clean the internal pipeline of the flow cytometry shell (10), and the cleaning agent is discharged after being circularly cleaned for unit turns, the cleaning agent is switched to output deionized water from the self-switching component (25), and the deionized water is circularly washed and discharged after being circularly cleaned for unit turns;
during cleaning, the temperature of the liquid is controlled by the temperature control part (23), the flow rate of the liquid is controlled by the flow detection part (24), the liquid is detected by the water quality detection part (33), and the circulating liquid is discharged when the pollutant is greater than a set value so as to be replaced by new circulating liquid;
and step three, drying the air flow, namely introducing a drying air flow into the cleaned pipeline through an air source part (26) so as to dry the interior of the pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111202329.2A CN113926775B (en) | 2021-10-15 | 2021-10-15 | Cleaning device and cleaning method for nozzle system of flow cytometry analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111202329.2A CN113926775B (en) | 2021-10-15 | 2021-10-15 | Cleaning device and cleaning method for nozzle system of flow cytometry analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113926775A true CN113926775A (en) | 2022-01-14 |
| CN113926775B CN113926775B (en) | 2023-05-12 |
Family
ID=79279728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111202329.2A Active CN113926775B (en) | 2021-10-15 | 2021-10-15 | Cleaning device and cleaning method for nozzle system of flow cytometry analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113926775B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114556086A (en) * | 2020-05-19 | 2022-05-27 | 生命科技股份有限公司 | Nozzle sealing and unclogging station for flow cytometer |
| CN114871210A (en) * | 2022-07-08 | 2022-08-09 | 深圳市帝迈生物技术有限公司 | Cleaning method of sample analyzer, sample analyzer and control device thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2233019Y (en) * | 1995-10-23 | 1996-08-14 | 秦皇岛市工程机械配套公司捷工管件技术研究所 | Card quick pipeline connector |
| CN101099969A (en) * | 2006-07-04 | 2008-01-09 | 深圳迈瑞生物医疗电子股份有限公司 | Wiper for cleaning sampling needle |
| CN104289466A (en) * | 2013-07-16 | 2015-01-21 | 成都深迈瑞医疗电子技术研究院有限公司 | Cell analyzer and cleaning system thereof |
| CN204564684U (en) * | 2015-04-30 | 2015-08-19 | 河南师范大学 | A kind of device interior pipeline cleaning system |
| CN108499950A (en) * | 2018-03-29 | 2018-09-07 | 郑州大学第附属医院 | A kind of ultrasonic cleaning equipment of medical treatment device |
| CN110420926A (en) * | 2019-08-16 | 2019-11-08 | 蒋书印 | A kind of multifunctional circulation cleaning system |
| CN111618054A (en) * | 2020-05-25 | 2020-09-04 | 湖北三江航天万峰科技发展有限公司 | Automatic cleaning system and method for water transporting tank |
| CN211563833U (en) * | 2019-12-30 | 2020-09-25 | 苏州中科医疗器械产业发展有限公司 | Be used for flow cytometry reagent needle belt cleaning device |
| CN213482012U (en) * | 2020-09-28 | 2021-06-18 | 刘洪立 | Quick cell analyzer with wash structure |
-
2021
- 2021-10-15 CN CN202111202329.2A patent/CN113926775B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2233019Y (en) * | 1995-10-23 | 1996-08-14 | 秦皇岛市工程机械配套公司捷工管件技术研究所 | Card quick pipeline connector |
| CN101099969A (en) * | 2006-07-04 | 2008-01-09 | 深圳迈瑞生物医疗电子股份有限公司 | Wiper for cleaning sampling needle |
| CN104289466A (en) * | 2013-07-16 | 2015-01-21 | 成都深迈瑞医疗电子技术研究院有限公司 | Cell analyzer and cleaning system thereof |
| CN204564684U (en) * | 2015-04-30 | 2015-08-19 | 河南师范大学 | A kind of device interior pipeline cleaning system |
| CN108499950A (en) * | 2018-03-29 | 2018-09-07 | 郑州大学第附属医院 | A kind of ultrasonic cleaning equipment of medical treatment device |
| CN110420926A (en) * | 2019-08-16 | 2019-11-08 | 蒋书印 | A kind of multifunctional circulation cleaning system |
| CN211563833U (en) * | 2019-12-30 | 2020-09-25 | 苏州中科医疗器械产业发展有限公司 | Be used for flow cytometry reagent needle belt cleaning device |
| CN111618054A (en) * | 2020-05-25 | 2020-09-04 | 湖北三江航天万峰科技发展有限公司 | Automatic cleaning system and method for water transporting tank |
| CN213482012U (en) * | 2020-09-28 | 2021-06-18 | 刘洪立 | Quick cell analyzer with wash structure |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114556086A (en) * | 2020-05-19 | 2022-05-27 | 生命科技股份有限公司 | Nozzle sealing and unclogging station for flow cytometer |
| CN114871210A (en) * | 2022-07-08 | 2022-08-09 | 深圳市帝迈生物技术有限公司 | Cleaning method of sample analyzer, sample analyzer and control device thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113926775B (en) | 2023-05-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113926775A (en) | Cleaning device and cleaning method for nozzle system of flow cytometry | |
| US20200300880A1 (en) | Waste liquid chamber, waste liquid treatment apparatus, and sample analyzer | |
| US11401952B2 (en) | Waste liquid treatment apparatus, method and sample analyzer | |
| CN107519790A (en) | A kind of liquid mixing system | |
| CN110644269A (en) | Industrial-grade automatic continuous steam explosion system and method | |
| CN112024551A (en) | Reation kettle washs scale removal system | |
| CN108241066A (en) | A kind of the inner loop pipeline system and its control method of more test solution Programmable detections | |
| CN104192960A (en) | Novel dosing device for powder medicament | |
| CN211532317U (en) | Automatic drinking water and integrative equipment of disinfection are with reducing pressure controlling means | |
| CN213480663U (en) | Automatic supply device for Freon of photoetching machine air conditioner | |
| CN208795738U (en) | A kind of water quality testing meter with automatic cleaning function | |
| CN108728331B (en) | Fermentation cylinder with self-cleaning device | |
| CN206838682U (en) | A kind of automatic cleaning system at monitoring water quality on line station | |
| CN112378287A (en) | Hot washing device for heat exchanger containing oil medium | |
| CN105514445A (en) | Full-automatic pickling system | |
| CN215587374U (en) | Aeration tubulation cleaning system for sewage treatment | |
| CN219935821U (en) | In-situ online evaluation device for lithium battery anode material liquid-phase treatment water | |
| CN112408576B (en) | Ozone heterogeneous catalytic oxidation comprehensive test platform and test method | |
| CN211328498U (en) | Large-flux filter element off-line cleaning system | |
| CN219573571U (en) | Cleaning and sampling device for storage tank | |
| CN212552112U (en) | Multifunctional full-automatic electrolyte supply equipment | |
| CN217084932U (en) | Integrated water quality monitoring equipment | |
| CN214585189U (en) | Liquid concentration detection system | |
| CN209476817U (en) | A kind of auto Resin washing machine | |
| CN217164441U (en) | Online automatic regenerating unit of power plant's hydrogen conductance ion exchange column |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |