CN113926775B - Cleaning device and cleaning method for nozzle system of flow cytometry analyzer - Google Patents
Cleaning device and cleaning method for nozzle system of flow cytometry analyzer Download PDFInfo
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- CN113926775B CN113926775B CN202111202329.2A CN202111202329A CN113926775B CN 113926775 B CN113926775 B CN 113926775B CN 202111202329 A CN202111202329 A CN 202111202329A CN 113926775 B CN113926775 B CN 113926775B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
- B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
- B08B9/0321—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing using pressurised, pulsating or purging fluid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/0804—Cleaning containers having tubular shape, e.g. casks, barrels, drums
- B08B9/0813—Cleaning containers having tubular shape, e.g. casks, barrels, drums by the force of jets or sprays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
- B08B9/28—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by splash, spray, or jet application, with or without soaking
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
Abstract
The invention discloses a cleaning device and a cleaning method of a flow cytometry nozzle system, wherein the cleaning device comprises a flow cytometry outer shell, a cleaning box and a sample tube, and the side wall of the sample tube is communicated with a threaded tube; a transmission pipe is arranged in the cleaning box, one end of the transmission pipe penetrates through the cleaning box to be connected with a mounting component, the mounting component is used for communicating the transmission pipe with a threaded pipe, a temperature control component and a flow detection component are arranged on the side wall of the transmission pipe, and a cleaning agent self-switching component and an air source component are arranged in the cleaning box; the side wall of the cleaning box is provided with a cleaning agent circulating device, the cleaning agent circulating device comprises a flow dividing component arranged at the liquid discharging end of the shell of the flow cytometry analyzer, a water quality detection component arranged on the outer wall of the cleaning box and connected with the flow dividing component through a circulating pipe, and a circulating component arranged on the inner wall of the cleaning box and used for circulating liquid in the water quality detection component into a transmission pipe. The invention provides a cleaning device and a cleaning method which are convenient for cleaning a sample tube and a nozzle system of an analyzer together.
Description
Technical Field
The invention mainly relates to the technical field of medical equipment cleaning, in particular to a cleaning device and a cleaning method for a nozzle system of a flow cytometry.
Background
The flow cytometer may rapidly measure, store, display a series of important biophysical, biochemical characteristics of dispersed cells suspended in a fluid and may sort out a designated subset of cells from the fluid based on a preselected range of parameters.
According to the full-automatic cleaning system of the flow cytometry provided by the patent document with the application number of CN201811651055.3 and the working method thereof, the system comprises a flushing liquid tank, a first cleaning pipeline, a first electromagnetic valve, a first cleaning branch, a second electromagnetic valve, a forehearth, a rear hearth, a jewel hole, a first liquid inlet pipe, a first liquid outlet pipe, a third electromagnetic valve, a sampling needle, a swab, a fourth electromagnetic valve, a second cleaning branch, a third cleaning branch, a group of reaction cup cleaning needles, a group of liquid suction needles, a second liquid discharge pipe and a waste liquid discharge outlet. The system can clean each unit of the cell analyzer, improves the use efficiency of the cleaning agent, can ensure the cleaning effect of the cell analyzer, and is safe and reliable to use.
The product in the above patent can carry out the washing of each unit of cell analyzer to improved the availability factor of cleaner, but be inconvenient for together wasing sample pipe and analyzer nozzle system, be inconvenient for controlling the washing liquid temperature velocity of flow, and be inconvenient for wasing and accomplish the back and carry out air-drying treatment.
Disclosure of Invention
The invention mainly provides a cleaning device and a cleaning method for a nozzle system of a flow cytometry analyzer, which are used for solving the technical problems in the background art.
The technical scheme adopted for solving the technical problems is as follows:
the cleaning device of the flow cytometry analyzer nozzle system comprises a flow cytometry analyzer shell, a cleaning box and a sample tube, wherein the side wall of the sample tube is communicated with a threaded tube, and a rubber ring is arranged on the inner wall of the threaded tube;
the cleaning box is internally provided with a transmission pipe, one end of the transmission pipe penetrates through the cleaning box to be connected with an installation component, the installation component is used for communicating the transmission pipe with a threaded pipe, the side wall of the transmission pipe is provided with a temperature control component and a flow detection component which are positioned in the cleaning box, the cleaning box is internally provided with a cleaning agent self-switching component and an air source component, and the execution ends of the cleaning agent self-switching component and the air source component are communicated with the transmission pipe;
the side wall of the cleaning box is provided with a cleaning agent circulating device, the cleaning agent circulating device comprises a flow dividing component arranged at the liquid discharging end of the shell of the flow cytometry, a water quality detection component arranged on the outer wall of the cleaning box and connected with the flow dividing component through a circulating pipe, and a circulating component arranged on the inner wall of the cleaning box and used for circulating liquid in the water quality detection component into a transmission pipe.
Preferably, the mounting component comprises a communicating pipe arranged at one end of the transmission pipe and a locking pipe rotationally connected with the outer wall of the communicating pipe, the communicating pipe penetrates through the rubber ring and extends into the sample pipe, and the inner wall of the locking pipe is in threaded connection with the outer wall of the threaded pipe. In the present preferred embodiment, stable communication of the transfer tube with the sample tube is facilitated by the mounting means.
Preferably, the temperature control component comprises a temperature sensor arranged on the inner wall of the transmission pipe, a positioning pipe sleeved on the outer wall of the transmission pipe, and a spiral heating pipe sleeved on the outer wall of the transmission pipe and positioned inside the positioning pipe. In the preferred embodiment, the control of the cleaning temperature is facilitated by the temperature control means.
Preferably, the flow detection component comprises a flow sensor arranged on the outer wall of the transmission pipe. In the preferred embodiment, the flow rate is conveniently controlled by the flow detection component, so that damage to the pipeline inside the shell of the flow rate counter flow cytometer is prevented.
Preferably, the cleaning agent self-switching component comprises a plurality of liquid storage tanks arranged at the bottom of the inner wall of the cleaning tank, an air pressure pipe communicated with the top of the liquid storage tanks and extending to the outside of the cleaning tank, a first electric control valve arranged at the liquid discharge end of the liquid storage tanks, and a feeding pipe communicated with a plurality of liquid discharge ends of the first electric control valve, wherein the feeding pipe is communicated with the transmission pipe through a pipeline. In the preferred embodiment, the introduction of different cleaning agents into the transfer tube is facilitated by the cleaning agent self-switching means.
Preferably, the air source component comprises an air storage tank arranged at the bottom of the inner wall of the cleaning tank, an air source pipe communicated with the top of the air storage tank and extending to the outside of the cleaning tank, and a second electric control valve arranged at the liquid discharge end of the air storage tank, and a liquid discharge end pipeline of the second electric control valve is communicated with the transmission pipe. In the preferred embodiment, the drying gas is conveniently introduced into the conveying pipe through the gas source component so as to facilitate the drying of the pipeline.
Preferably, the shunt part is including locating the shunt tubes of flow cytometry outer casing flowing back end, shunt tubes outer wall intercommunication blow off pipe and circulating pipe, the shunt tubes is kept away from the one end of flow cytometry outer casing is equipped with the actuating cylinder, actuating cylinder execution end runs through shunt tubes connection plunger plate, plunger plate lateral wall is equipped with the closing plate, be equipped with the through-hole on the closing plate, the through-hole with the circulating pipe position corresponds. In the preferred embodiment, circulation of the cleaning agent during cleaning is facilitated by the flow dividing member, and normal pollution discharge is performed during use of the flow cytometer casing.
Preferably, the water quality detection part comprises a water quality detection box arranged on the outer wall of the cleaning box, a groove arranged on the bottom of the inner wall of the water quality detection box, a water quality sensor arranged on the bottom of the groove, and a drain pipe which is communicated with the side wall of the water quality detection box and is positioned at the bottom of the side wall of the groove, wherein a third electric control valve is arranged on the drain pipe, and the side wall of the water quality detection box is communicated with the circulating pipe. In the preferred embodiment, detection of the cleaning agent is facilitated by the water quality detection means and the circulating fluid is discharged when the contaminants are greater than the set value.
Preferably, the circulating component comprises a circulating pump arranged on the inner wall of the cleaning box, a liquid inlet end of the circulating pump is communicated with the water quality detection box through a pipeline, and a liquid discharge end of the circulating pump is communicated with the transmission pipe through a pipeline. In the present preferred embodiment, the circulation of the cleaning agent is facilitated by the circulation means.
According to the above technical solution of the cleaning device of the nozzle system of the flow cytometer, there is also provided a cleaning method of the cleaning device of the nozzle system of the flow cytometer, including the steps of:
step one, connecting a device, namely communicating a transmission pipe with a sample pipe through a mounting component, and placing the sample pipe at a sampling position of a shell of a flow cytometry analyzer;
step two, circulating cleaning, namely introducing a certain amount of cleaning agent into the transmission pipe through the cleaning agent from the switching part, and forming circulation after sequentially passing through the transmission pipe, the sample pipe, the flow cytometry outer shell, the flow dividing part, the water quality detection part and the circulating part, so as to circularly clean the inner pipeline of the flow cytometry outer shell, discharging the cleaning agent after circularly cleaning unit turns, switching the cleaning agent from the switching part to output deionized water, circularly flushing the inner pipeline of the flow cytometry outer shell by the deionized water, and discharging the cleaning agent after circularly cleaning unit turns;
during cleaning, the temperature of the liquid is controlled through the temperature control component, the flow rate of the liquid is controlled through the flow detection component, the liquid is detected through the water quality detection component, and the circulating liquid is discharged when the pollutant is greater than a set value so as to replace new circulating liquid;
and thirdly, air-drying, namely introducing a drying air flow into the cleaned pipeline through the air source component so as to air-dry the interior of the pipeline.
Compared with the prior art, the invention has the beneficial effects that:
the cleaning device is convenient for cleaning the sample tube and the nozzle system of the analyzer, is convenient for controlling the temperature and the flow rate of the cleaning liquid, and is convenient for air drying treatment after cleaning;
the utility model discloses a flow cytometer shell is convenient for carry out the circulation of cleaning agent when wasing, normal blowdown when the flow cytometer shell is used, be convenient for detect the cleaning agent through water quality testing part and discharge circulating fluid when the pollutant is greater than the setting value, be convenient for circulate the cleaning agent through circulating part, be convenient for control cleaning temperature through accuse temperature part, be convenient for control the velocity of flow through flow testing part, prevent that the too fast convection type cytometer shell internal pipeline of velocity of flow from producing the damage.
The invention will be explained in detail below with reference to the drawings and specific embodiments.
Drawings
FIG. 1 is an isometric view of the overall structure of the present invention;
FIG. 2 is an exploded view of the overall structure of the present invention;
FIG. 3 is an isometric view of the structure of the cleaning agent circulation device of the present invention;
FIG. 4 is an isometric view of the internal structure of the purge bin of the present invention;
FIG. 5 is a top view of the overall structure of the present invention;
FIG. 6 is a cross-sectional view of the overall structure of the present invention;
FIG. 7 is a cross-sectional view of a transfer tube structure of the present invention;
FIG. 8 is a cross-sectional view showing the construction of a water quality detecting unit according to the present invention.
Description of the drawings: 10. a flow cytometer housing; 20. a cleaning box; 21. a transmission tube; 22. a mounting member; 221. a communicating pipe; 222. locking the tube; 23. a temperature control member; 231. a temperature sensor; 232. a positioning tube; 233. a spiral heating pipe; 24. a flow rate detection unit; 241. a flow sensor; 25. a cleaning agent self-switching member; 251. a liquid storage tank; 252. an air pressure pipe; 253. a first electrically controlled valve; 254. a feed pipe; 26. an air source component; 261. a gas storage tank; 262. an air source pipe; 263. a second electrically controlled valve; 30. a cleaning agent circulation device; 31. a shunt member; 311. a shunt; 312. a blow-down pipe; 313. a drive cylinder; 314. a plunger plate; 315. a sealing plate; 316. a through hole; 32. a circulation pipe; 33. a water quality detection unit; 331. a water quality detection tank; 332. a groove; 333. a water quality sensor; 334. a drain tube; 335. a third electrically controlled valve; 34. a circulating member; 341. a circulation pump; 40. a sample tube; 41. a threaded tube; 42. a rubber ring.
Detailed Description
In order that the invention may be more fully understood, a more particular description of the invention will be rendered by reference to the appended drawings, in which several embodiments of the invention are illustrated, but which may be embodied in different forms and are not limited to the embodiments described herein, which are, on the contrary, provided to provide a more thorough and complete disclosure of the invention.
It will be understood that when an element is referred to as being "mounted" on another element, it can be directly on the other element or intervening elements may be present, and when an element is referred to as being "connected" to the other element, it may be directly connected to the other element or intervening elements may also be present, the terms "vertical", "horizontal", "left", "right" and the like are used herein for the purpose of illustration only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly connected to one of ordinary skill in the art to which this invention belongs, and the knowledge of terms used in the description of this invention herein for the purpose of describing particular embodiments is not intended to limit the invention, and the term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1, 2 and 7, in a preferred embodiment of the present invention, a cleaning device for a nozzle system of a flow cytometer includes a flow cytometer housing 10, a cleaning box 20 and a sample tube 40, wherein a side wall of the sample tube 40 is communicated with a threaded tube 41, and a rubber ring 42 is disposed on an inner wall of the threaded tube 41; the mounting member 22 includes a communicating pipe 221 provided at one end of the transmission pipe 21, and a locking pipe 222 rotatably connected to an outer wall of the communicating pipe 221, the communicating pipe 221 penetrates the rubber ring 42 and extends into the sample tube 40, and an inner wall of the locking pipe 222 is screwed to an outer wall of the threaded tube 41.
In the present embodiment, the transport tube 21 is connected to the sample tube 40 by the mounting member 22, and the sample tube 40 is placed at the sampling position of the flow cytometer casing 10, that is, the sample tube 40 is fitted to the outside of the nozzle of the flow cytometer casing 10;
further, when the mounting member 22 is used, the communicating tube 221 is inserted into the sample tube 40 through the rubber ring 42, and the locking tube 222 is rotated so that the inner wall of the locking tube 222 is screwed to the outer wall of the screw tube 41.
Referring to fig. 2, 3, 4, 6, and 7, in another preferred embodiment of the present invention, a transmission pipe 21 is disposed in the cleaning tank 20, one end of the transmission pipe 21 penetrates through the cleaning tank 20 to connect with a mounting component 22, the mounting component 22 is used for communicating the transmission pipe 21 with a threaded pipe 41, a temperature control component 23 and a flow detection component 24 are disposed on a sidewall of the transmission pipe 21 and in the cleaning tank 20, a cleaning agent self-switching component 25 and an air source component 26 are disposed in the cleaning tank 20, and execution ends of the cleaning agent self-switching component 25 and the air source component 26 are both communicated with the transmission pipe 21; the temperature control part 23 comprises a temperature sensor 231 arranged on the inner wall of the transmission pipe 21, a positioning pipe 232 arranged on the outer wall of the transmission pipe 21 in a sleeved mode, and a spiral heating pipe 233 arranged on the outer wall of the transmission pipe 21 and positioned in the positioning pipe 232, the flow detection part 24 comprises a flow sensor 241 arranged on the outer wall of the transmission pipe 21, the cleaning agent self-switching part 25 comprises a plurality of liquid storage tanks 251 arranged on the bottom of the inner wall of the cleaning tank 20, an air pressure pipe 252 communicated with the top of the liquid storage tanks 251 and extending to the outside of the cleaning tank 20, a first electric control valve 253 arranged at the liquid discharge end of the liquid storage tanks 251, and a feed pipe 254 communicated with the liquid discharge end of the first electric control valve 253, the feed pipe 254 is communicated with the transmission pipe 21 through a pipeline, the air source part 26 comprises an air storage tank 261 arranged on the bottom of the inner wall of the cleaning tank 20, an air source pipe 262 communicated with the top of the air storage tank 261 and extending to the outside of the cleaning tank 20, and a second electric control valve 263 arranged at the liquid discharge end of the air storage tank 261, and the second electric control valve 263 is communicated with the transmission pipe 21 through a pipeline.
In this embodiment, a certain amount of cleaning agent, such as a flow cytometer cleaning agent, is introduced into the transmission tube 21 from the switching component 25, and sequentially passes through the transmission tube 21, the sample tube 40, the flow cytometer housing 10, the flow dividing component 31, the water quality detection component 33 and the circulation component 34 to form circulation, so that the internal pipeline of the flow cytometer housing 10 is circularly cleaned, and then discharged after a unit number of circles is circularly cleaned, the cleaning agent is switched from the switching component 25 to output another cleaning agent, such as deionized water, the internal pipeline of the flow cytometer housing 10 is circularly cleaned by deionized water, and then discharged after a unit number of circles is circularly cleaned, and then a dry air flow is introduced into the cleaned pipeline by the air source component 26 to air-dry the internal pipeline;
during cleaning, the temperature of the liquid is controlled by the temperature control part 23, and the flow rate of the liquid is controlled by the flow detection part 24;
further, when the cleaning agent works from the switching component 25, the first electric control valve 253 corresponding to the liquid storage tank 251 for storing the flow cytometer cleaning agent is opened, at this time, the flow cytometer cleaning agent enters the transmission pipe 21 through the feeding pipe 254, when the cleaning agent is switched, the first electric control valve 253 corresponding to the liquid storage tank 251 for storing the flow cytometer cleaning agent is closed, and the first electric control valve 253 corresponding to the liquid storage tank 251 for storing the deionized water is opened, so that the switching can be completed, and the air pressure pipe 252 is convenient for inputting pressure air into the liquid storage tank 251 so as to facilitate the feeding of the cleaning agent;
further, when the air source component 26 works, the air source pipe 262 can input a dry air source into the air storage tank 261, and the second electric control valve 263 is opened, so that the dry air enters the transmission pipe 21;
further, when the temperature control component 23 works, the PLC controller receives the temperature data of the temperature sensor 231 and triggers the spiral heating pipe 233 until the temperature data is consistent with the set value;
further, when the flow rate detecting unit 24 is operated, the PLC controller receives the flow rate data of the flow rate sensor 241 and changes the power of the circulation pump 341 until the flow rate data is identical to the set value.
Referring to fig. 2, 5, 6 and 8, in another preferred embodiment of the present invention, a cleaning agent circulation device 30 is disposed on a side wall of the cleaning tank 20, the cleaning agent circulation device 30 includes a diversion member 31 disposed on a liquid discharge end of the flow cytometer housing 10, a water quality detection member 33 disposed on an outer wall of the cleaning tank 20 and connected to the diversion member 31 through a circulation pipe 32, and a circulation member 34 disposed on an inner wall of the cleaning tank 20 and configured to circulate a liquid in the water quality detection member 33 into the transmission tube 21, the diversion member 31 includes a diversion tube 311 disposed on the liquid discharge end of the flow cytometer housing 10, the outer wall of the diversion tube 311 is communicated with a drain tube 312 and the circulation pipe 32, a driving cylinder 313 is disposed on an end of the diversion tube 311 remote from the flow cytometer housing 10, an execution end of the driving cylinder 313 is connected to a plunger plate 314, a sealing plate 316 is disposed on a side wall of the plunger plate 314, the sealing plate 315 is disposed with a through hole 316, the through hole 316 corresponds to the position of the circulation pipe 32, the water quality detection member 33 includes a diversion tube 311 disposed on an outer wall of the cleaning tank 331, a water quality detection member 331 is disposed on a side wall of the plunger plate 331 and is disposed on a bottom of the circulation tube 331, and is communicated with a bottom portion of the transmission tube 33, and is disposed on a bottom portion of the transmission tube 33, which is communicated with a bottom portion of the transmission tube 33, and is disposed on a bottom portion of the transmission tube 33.
In this embodiment, the flow dividing member 31 facilitates circulation of the cleaning agent during cleaning, normal sewage is discharged during use of the flow cytometer housing 10, and the water quality detecting member 33 detects the liquid and discharges the circulating liquid when the contaminant is greater than the set value to replace the circulating liquid with a new one;
further, when the flow dividing member 31 works, when the through hole 316 is overlapped with the circulation pipe 32, the plunger plate 314 seals the drain pipe 312, at this time, liquid enters the circulation pipe 32 through the through hole 316, when the driving cylinder 313 drives the plunger plate 314 to move, the through hole 316 is dislocated with the circulation pipe 32, at this time, the plunger plate 314 cancels the seal of the drain pipe 312, and the liquid is discharged through the drain pipe 312;
further, when the water quality detecting part 33 works, the cleaning fluid discharged through the circulating pipe 32 enters the water quality detecting box 331 and fills the groove 332, the water quality sensor 333 transmits the water quality information to the PLC controller, the PLC controller starts the circulating pump 341 when the amount of the contaminant is smaller than the set value after analyzing the water quality information, so as to circulate the cleaning fluid, and closes the circulating pump 341 when the amount of the contaminant is larger than the set value, and opens the third electric control valve 335, so as to discharge the cleaning fluid through the drain pipe 334.
According to the above embodiment, there will also be provided a cleaning method of a cleaning device of a nozzle system of a flow cytometer, including the steps of:
step one, connecting the device, namely communicating the transmission tube 21 with the sample tube 40 through the mounting component 22, and placing the sample tube 40 at the sampling position of the flow cytometry housing 10;
step two, circulating cleaning, namely introducing a certain amount of cleaning agent into the transmission pipe 21 through the cleaning agent from the switching part 25, and circulating the cleaning agent through the transmission pipe 21, the sample pipe 40, the flow cytometry housing 10, the flow dividing part 31, the water quality detection part 33 and the circulating part 34 in sequence to circularly clean the inner pipeline of the flow cytometry housing 10, discharging the cleaning agent after circularly cleaning the unit number of turns, switching the cleaning agent from the switching part 25 to output deionized water, circularly flushing the inner pipeline of the flow cytometry housing 10 through the deionized water, and discharging the cleaning agent after circularly cleaning the unit number of turns;
during cleaning, the temperature of the liquid is controlled by the temperature control part 23, the flow rate of the liquid is controlled by the flow detection part 24, the liquid is detected by the water quality detection part 33, and the circulating liquid is discharged when the pollutant is greater than a set value so as to replace the circulating liquid with a new circulating liquid;
and thirdly, air-drying, namely introducing a drying air flow into the cleaned pipeline through the air source component 26 so as to air-dry the interior of the pipeline.
The specific flow of the invention is as follows:
the model of the PLC is '6 ES7214-1BD23-0XB 8', the model of the temperature sensor 231 is 'DS 18B 20', the model of the flow sensor 241 is 'CX', and the model of the water quality sensor 333 is 'XZ 3010'.
The transmission tube 21 is communicated with the sample tube 40 through the mounting part 22, and the sample tube 40 is placed at the sampling position of the flow cytometer casing 10 at this time, namely, the sample tube 40 is sleeved outside the nozzle of the flow cytometer casing 10;
when the mounting component 22 is used, the communicating pipe 221 penetrates through the rubber ring 42 and is inserted into the sample tube 40, and the locking tube 222 is rotated, so that the inner wall of the locking tube 222 is in threaded connection with the outer wall of the threaded tube 41;
a certain amount of cleaning agent, such as a flow cytometer cleaning agent, is introduced into the transmission pipe 21 through the cleaning agent self-switching part 25, and sequentially passes through the transmission pipe 21, the sample pipe 40, the flow cytometer housing 10, the flow dividing part 31, the water quality detection part 33 and the circulation part 34 to form circulation so as to circularly clean the internal pipeline of the flow cytometer housing 10, the cleaning agent is discharged after the circulation cleaning unit turns, the cleaning agent is switched from the switching part 25 to output another cleaning agent, such as deionized water, the deionized water circularly washes the internal pipeline of the flow cytometer housing 10, the cleaning agent is discharged after the circulation unit turns, and a dry air flow is introduced into the cleaned pipeline through the air source part 26 after the cleaning is completed so as to air-dry the internal pipeline;
during cleaning, the temperature of the liquid is controlled by the temperature control part 23, and the flow rate of the liquid is controlled by the flow detection part 24;
when the cleaning agent self-switching component 25 works, the first electric control valve 253 corresponding to the liquid storage tank 251 for storing the flow cytometer cleaning agent in the interior is opened, at this time, the flow cytometer cleaning agent enters the transmission pipe 21 through the feed pipe 254, when the cleaning agent is switched, the first electric control valve 253 corresponding to the liquid storage tank 251 for storing the flow cytometer cleaning agent in the interior is closed, the first electric control valve 253 corresponding to the liquid storage tank 251 for storing deionized water in the interior is opened, the switching can be completed, and the air pressure pipe 252 is convenient for inputting pressure air into the liquid storage tank 251 so as to facilitate the feeding of the cleaning agent;
when the air source component 26 works, the air source pipe 262 can input a dry air source into the air storage tank 261, the second electric control valve 263 is opened, and the dry air enters the transmission pipe 21;
when the temperature control component 23 works, the PLC receives temperature data of the temperature sensor 231 and triggers the spiral heating pipe 233 until the temperature data is consistent with a set value;
when the flow detection component 24 works, the PLC receives flow data of the flow sensor 241 and changes the power of the circulating pump 341 until the flow data is consistent with a set value;
the flow dividing part 31 facilitates circulation of the cleaning agent during cleaning, normal sewage discharge is performed when the flow cytometer housing 10 is used, the water quality detecting part 33 detects the liquid and discharges the circulating liquid when the pollutant is greater than a set value to replace the circulating liquid with a new one;
when the flow dividing component 31 works, when the through hole 316 is overlapped with the circulating pipe 32, the plunger plate 314 seals the drain pipe 312, at the moment, liquid enters the circulating pipe 32 through the through hole 316, when the driving cylinder 313 drives the plunger plate 314 to move, the through hole 316 is misplaced with the circulating pipe 32, at the moment, the plunger plate 314 cancels the sealing of the drain pipe 312, and the liquid is discharged through the drain pipe 312;
when the water quality detecting part 33 works, the cleaning liquid discharged through the circulating pipe 32 enters the water quality detecting box 331 and fills the groove 332, the water quality sensor 333 transmits the water quality information to the PLC controller, the PLC controller starts the circulating pump 341 when the pollutant amount is smaller than a set value after analyzing the water quality information so as to circulate the cleaning agent, and closes the circulating pump 341 when the pollutant amount is larger than the set value, and opens the third electric control valve 335 so as to discharge the cleaning agent through the drain pipe 334.
While the invention has been described above with reference to the accompanying drawings, it will be apparent that the invention is not limited to the embodiments described above, but is intended to be within the scope of the invention, as long as such insubstantial modifications are made by the method concepts and technical solutions of the invention, or the concepts and technical solutions of the invention are applied directly to other occasions without any modifications.
Claims (2)
1. The cleaning device of the flow cytometry analyzer nozzle system comprises a flow cytometry analyzer shell (10), a cleaning box (20) and a sample tube (40), and is characterized in that the side wall of the sample tube (40) is communicated with a threaded tube (41), and a rubber ring (42) is arranged on the inner wall of the threaded tube (41);
the cleaning box is characterized in that a transmission pipe (21) is arranged in the cleaning box (20), one end of the transmission pipe (21) penetrates through the cleaning box (20) to be connected with an installation component (22), the installation component (22) is used for communicating the transmission pipe (21) with a threaded pipe (41), a temperature control component (23) and a flow detection component (24) are arranged on the side wall of the transmission pipe (21) and located in the cleaning box (20), a cleaning agent self-switching component (25) and an air source component (26) are arranged in the cleaning box (20), and the execution ends of the cleaning agent self-switching component (25) and the air source component (26) are communicated with the transmission pipe (21);
the side wall of the cleaning box (20) is provided with a cleaning agent circulating device (30), the cleaning agent circulating device (30) comprises a flow dividing component (31) arranged at the liquid discharging end of the shell (10) of the flow cytometry, a water quality detection component (33) arranged on the outer wall of the cleaning box (20) and connected with the flow dividing component (31) through a circulating pipe (32), and a circulating component (34) arranged on the inner wall of the cleaning box (20) and used for circulating liquid in the water quality detection component (33) into a transmission pipe (21);
the mounting component (22) comprises a communicating pipe (221) arranged at one end of the transmission pipe (21) and a locking pipe (222) rotatably connected with the outer wall of the communicating pipe (221), the communicating pipe (221) penetrates through the rubber ring (42) and extends into the sample pipe (40), and the inner wall of the locking pipe (222) is in threaded connection with the outer wall of the threaded pipe (41);
the cleaning agent self-switching component (25) comprises a plurality of liquid storage tanks (251) arranged at the bottom of the inner wall of the cleaning tank (20), an air pressure pipe (252) communicated with the top of the liquid storage tanks (251) and extending to the outside of the cleaning tank (20), a first electric control valve (253) arranged at the liquid discharge end of the liquid storage tanks (251), and a feeding pipe (254) communicated with the liquid discharge ends of the first electric control valves (253), wherein the feeding pipe (254) is communicated with the transmission pipe (21) through a pipeline;
the temperature control component (23) comprises a temperature sensor (231) arranged on the inner wall of the transmission pipe (21), a positioning pipe (232) sleeved on the outer wall of the transmission pipe (21), and a spiral heating pipe (233) sleeved on the outer wall of the transmission pipe (21) and positioned in the positioning pipe (232);
the flow detection component (24) comprises a flow sensor (241) arranged on the outer wall of the transmission pipe (21);
the air source component (26) comprises an air storage tank (261) arranged at the bottom of the inner wall of the cleaning tank (20), an air source pipe (262) communicated with the top of the air storage tank (261) and extending to the outside of the cleaning tank (20), and a second electric control valve (263) arranged at the liquid discharge end of the air storage tank (261), wherein the liquid discharge end pipe of the second electric control valve (263) is communicated with the transmission pipe (21);
the flow dividing component (31) comprises a flow dividing pipe (311) arranged at the liquid discharging end of the flow cytometry analyzer shell (10), the outer wall of the flow dividing pipe (311) is communicated with a drain pipe (312) and a circulating pipe (32), one end, far away from the flow cytometry analyzer shell (10), of the flow dividing pipe (311) is provided with a driving cylinder (313), the execution end of the driving cylinder (313) penetrates through the flow dividing pipe (311) to be connected with a plunger plate (314), the side wall of the plunger plate (314) is provided with a sealing plate (315), the sealing plate (315) is provided with a through hole (316), and the through hole (316) corresponds to the position of the circulating pipe (32);
the water quality detection component (33) comprises a water quality detection box (331) arranged on the outer wall of the cleaning box (20), a groove (332) arranged at the bottom of the inner wall of the water quality detection box (331), a water quality sensor (333) arranged at the bottom of the groove (332), and a drain pipe (334) communicated with the side wall of the water quality detection box (331) and positioned at the bottom of the side wall of the groove (332), wherein a third electric control valve (335) is arranged on the drain pipe (334), and the side wall of the water quality detection box (331) is communicated with the circulating pipe (32);
the circulating component (34) comprises a circulating pump (341) arranged on the inner wall of the cleaning box (20), a liquid inlet end of the circulating pump (341) is communicated with the water quality detection box (331) through a pipeline, and a liquid outlet end of the circulating pump is communicated with the transmission pipe (21) through a pipeline.
2. The method for cleaning a cleaning device for a nozzle system of a flow cytometer of claim 1, comprising the steps of:
step one, connecting the device, namely communicating a transmission pipe (21) with a sample pipe (40) through a mounting component (22), and placing the sample pipe (40) at a sampling position of a shell (10) of the flow cytometer;
step two, circularly cleaning, namely introducing a certain amount of cleaning agent into the transmission pipe (21) through the cleaning agent from the switching part (25), and circularly cleaning the inner pipeline of the flow cytometry housing (10) through the transmission pipe (21), the sample pipe (40), the flow cytometry housing (10), the flow dividing part (31), the water quality detection part (33) and the circulation part (34) sequentially to obtain a circulation, discharging after circularly cleaning the inner pipeline of the flow cytometry housing (10) for unit circles, switching the cleaning agent from the switching part (25) to output deionized water, circularly flushing the inner pipeline of the flow cytometry housing (10) through the deionized water, and discharging after circularly cleaning the unit circles;
during cleaning, the temperature of the liquid is controlled by a temperature control component (23), the flow rate of the liquid is controlled by a flow detection component (24), the liquid is detected by a water quality detection component (33), and the circulating liquid is discharged when the pollutant is greater than a set value so as to replace new circulating liquid;
and thirdly, air-drying, namely introducing a drying air flow into the cleaned pipeline through an air source component (26) so as to air-dry the interior of the pipeline.
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| CN114871210B (en) * | 2022-07-08 | 2022-10-28 | 深圳市帝迈生物技术有限公司 | Cleaning method of sample analyzer, sample analyzer and control device thereof |
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| CN2233019Y (en) * | 1995-10-23 | 1996-08-14 | 秦皇岛市工程机械配套公司捷工管件技术研究所 | Card quick pipeline connector |
| CN100581663C (en) * | 2006-07-04 | 2010-01-20 | 深圳迈瑞生物医疗电子股份有限公司 | Wiper for cleaning sampling needle |
| CN104289466B (en) * | 2013-07-16 | 2017-03-15 | 成都深迈瑞医疗电子技术研究院有限公司 | Cytoanalyze and its purging system |
| CN204564684U (en) * | 2015-04-30 | 2015-08-19 | 河南师范大学 | A kind of device interior pipeline cleaning system |
| CN108499950B (en) * | 2018-03-29 | 2020-04-28 | 郑州大学第一附属医院 | Ultrasonic cleaning device of medical device |
| CN211563833U (en) * | 2019-12-30 | 2020-09-25 | 苏州中科医疗器械产业发展有限公司 | Be used for flow cytometry reagent needle belt cleaning device |
| CN111618054B (en) * | 2020-05-25 | 2022-03-08 | 湖北三江航天万峰科技发展有限公司 | Automatic cleaning system and method for water transporting tank |
| CN213482012U (en) * | 2020-09-28 | 2021-06-18 | 刘洪立 | Quick cell analyzer with wash structure |
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