CN113109583B - Flow cytometer sample test tube processing device - Google Patents
Flow cytometer sample test tube processing device Download PDFInfo
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- CN113109583B CN113109583B CN202110393998.6A CN202110393998A CN113109583B CN 113109583 B CN113109583 B CN 113109583B CN 202110393998 A CN202110393998 A CN 202110393998A CN 113109583 B CN113109583 B CN 113109583B
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- 238000004140 cleaning Methods 0.000 claims abstract description 60
- 239000002699 waste material Substances 0.000 claims abstract description 54
- 238000002955 isolation Methods 0.000 claims abstract description 53
- 238000005070 sampling Methods 0.000 claims abstract description 53
- 239000007788 liquid Substances 0.000 claims abstract description 45
- 230000002093 peripheral effect Effects 0.000 claims abstract description 20
- 239000002912 waste gas Substances 0.000 claims abstract description 14
- 238000002347 injection Methods 0.000 claims description 23
- 239000007924 injection Substances 0.000 claims description 23
- 238000004659 sterilization and disinfection Methods 0.000 claims description 19
- 238000011084 recovery Methods 0.000 claims description 12
- 239000000463 material Substances 0.000 claims 1
- 230000002411 adverse Effects 0.000 abstract description 6
- 238000000684 flow cytometry Methods 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 6
- 239000006285 cell suspension Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000012530 fluid Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 238000005299 abrasion Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000012459 cleaning agent Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000009652 hydrodynamic focusing Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- -1 tube 8 cleaning Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B15/00—Preventing escape of dirt or fumes from the area where they are produced; Collecting or removing dirt or fumes from that area
- B08B15/04—Preventing escape of dirt or fumes from the area where they are produced; Collecting or removing dirt or fumes from that area from a small area, e.g. a tool
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
- B08B9/28—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by splash, spray, or jet application, with or without soaking
- B08B9/30—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by splash, spray, or jet application, with or without soaking and having conveyors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
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- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Robotics (AREA)
- Mechanical Engineering (AREA)
- Dispersion Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a flow cytometer sample tube processing device, comprising: a body having an isolation cavity; the sampling box is arranged in the isolation cavity and is provided with a sampling cavity; the sample moving device is arranged in the sampling cavity; the manipulator is arranged in the sampling cavity and is matched with the sample moving device; the suction device is arranged in the isolation cavity and comprises a connecting column and a sleeve which are connected, the sleeve is located right above the manipulator, one side of the sleeve, which is close to the manipulator, is provided with a suction pipe and a cleaning pipe, the suction device further comprises a discharge pipe, and a discharge hole communicated with the discharge pipe is formed in the peripheral wall of the connecting column. The flow cytometer sample tube treatment device provided by the invention can timely recover and treat waste liquid and waste gas generated after sample introduction, and clean the test tube, so as to avoid experimental environment pollution caused by volatilizing the residual sample in the test tube into the air and prevent operators or experimental instruments from being adversely affected.
Description
Technical Field
The invention relates to the technical field of flow cytometry, in particular to a flow cytometry sample tube processing device.
Background
The flow cytometer is an instrument for automatically analyzing and sorting cells, which can rapidly measure, store and display a series of important characteristic parameters of dispersed cells suspended in a liquid, and sort cell subsets according to a preselected parameter range. The working principle of the flow cytometer is that the cell to be measured is made into a single cell suspension sample, the single cell suspension sample is pressed into a flow chamber by adopting a certain pressure, meanwhile, a sheath liquid is added at the periphery of the single cell suspension liquid flow, the flow direction of the sheath liquid is a certain angle with the single cell suspension liquid flow, so that the sheath liquid flows around the sample at a high speed, the single cell suspension liquid forms hydrodynamic focusing under the ring package of the sheath liquid flow, and the metabolic cells are arranged in a single row along the axis direction of the liquid flow so as to pass through a laser detection area.
The existing flow cytometry generally adopts a simple mechanical device for sample injection or artificial sample injection, a sample is usually exposed in an external environment, residual waste liquid in a test tube after the sample is introduced and waste gas discharged from a catheter for sucking the sample in the test tube cannot be timely recycled, the test tube cannot be timely cleaned in the experimental process, partial substances in the sample are scattered into the air to pollute the experimental environment, and the risk of adverse effects on test instruments and operators exists. There is therefore a need for a flow cytometer sample tube processing apparatus that addresses the above-described issues.
Disclosure of Invention
The technical problems to be solved by the invention are as follows: provided is a flow cytometer sample tube processing apparatus capable of effectively processing waste liquid and waste gas remaining after sample introduction.
In order to solve the technical problems, the invention adopts the following technical scheme: a flow cytometer sample tube processing apparatus comprising: a body having an isolation cavity; the sampling box is arranged in the isolation cavity and is provided with a sampling cavity; the sample moving device is arranged in the sampling cavity; the manipulator is arranged in the sampling cavity and is matched with the sample moving device; the suction device is arranged in the isolation cavity and comprises a connecting column and a sleeve which are connected, the sleeve is positioned right above the manipulator, one side, close to the manipulator, of the sleeve is provided with a suction pipe and a cleaning pipe, the suction device further comprises a discharge pipe, and a discharge hole communicated with the discharge pipe is formed in the peripheral wall of the connecting column.
The invention has the beneficial effects that: the flow cytometer sample tube processing device provided by the invention is characterized in that a sample moving device is used for placing a test tube outside a main body and injecting sample in a closed isolation cavity, a mechanical arm is specifically adopted in the sample injection process to move the test tube to enable an opening of the test tube to be matched with a sleeve and a part of connecting column in an absorbing device, then the sample in the test tube is absorbed by the absorbing tube to sample through the absorbing tube, a cleaning tube is used for injecting cleaning liquid into the absorbing tube and cleaning the test tube from bottom to top, then a discharging tube is used for absorbing and storing waste liquid in the test tube and waste gas discharged by the absorbing tube, timely recovery and treatment of waste liquid and waste gas generated after sample introduction are realized, and the test tube is cleaned at the first time after sample injection is completed, so that experimental environment pollution caused by volatilization of residual sample in the test tube to air is avoided, and operators or experimental instruments are prevented from being adversely affected.
Drawings
FIG. 1 is a schematic view of a flow cytometer sample tube processing apparatus with an isolation cover in a closed position;
FIG. 2 is a schematic diagram showing the structure of an isolation cover in an open state in a flow cytometer sample tube processing apparatus according to an embodiment of the present invention;
FIG. 3 is a schematic view showing a part of a flow cytometer sample tube processing apparatus according to an embodiment of the present invention;
FIG. 4 is a schematic view of a portion of a suction device in a flow cytometer sample tube processing apparatus according to an embodiment of the present invention;
FIG. 5 is a cross-sectional view of a portion of a sample tube handling apparatus for a flow cytometer in accordance with an embodiment of the present invention;
FIG. 6 is an enlarged view at A in FIG. 5;
FIG. 7 is a schematic view of a sample moving apparatus in a flow cytometer sample tube processing apparatus according to an embodiment of the present invention;
FIG. 8 is a cross-sectional view showing a part of the structure of a flow cytometer sample tube processing apparatus according to the first embodiment of the present invention;
FIG. 9 is a schematic view showing the structure of a suction device in a flow cytometer sample tube processing apparatus in accordance with the first embodiment of the present invention;
FIG. 10 is a cross-sectional view of a portion of a suction device in a flow cytometer sample tube processing apparatus in accordance with a second embodiment of the present invention.
Description of the reference numerals:
1. a main body; 100. a flow chamber; 101. an isolation cover; 2. a sampling box; 201. a sampling cavity; 202. a disinfection chamber; 3. a controller; 4. a waste bin; 5. a liquid storage tank; 6. a sample moving device; 601. a placement groove; 602. a boss; 7. a suction box; 8. a test tube; 9. a manipulator; 10. a first sensor; 11. suction means; 111. a mounting base; 112. a second sensor; 113. a discharge hole; 114. a connecting column; 115. an air-filling sleeve; 1151. an inflator pump; 116. a sleeve; 117. cleaning the tube; 118. a suction pipe; 119. a discharge pipe; 12. a sterilizing lamp; 120. a feeding pump; 121. a cleaning pump; 122. a waste pump; 13. a servo electric cylinder; 14. a connecting rod; 15. and an isolation door.
Detailed Description
In order to describe the technical contents, the achieved objects and effects of the present invention in detail, the following description will be made with reference to the embodiments in conjunction with the accompanying drawings.
Referring to fig. 1 to 10, a flow cytometer sample tube processing apparatus includes: a main body 1 having an isolation chamber; a sampling box 2 arranged in the isolation cavity and provided with a sampling cavity 201; a sample moving means 6 disposed within the sampling chamber 201; the manipulator 9 is arranged in the sampling cavity 201 and matched with the sample moving device 6; the suction device 11 is arranged in the isolation cavity and comprises a connecting column 114 and a sleeve 116 which are connected, the sleeve 116 is positioned right above the manipulator 9, one side of the sleeve 116, which is close to the manipulator 9, is provided with a suction pipe 118 and a cleaning pipe 117, and further comprises a discharge pipe 119, and the peripheral wall of the connecting column 114 is provided with a discharge hole 113 communicated with the discharge pipe 119.
The working principle of the invention is briefly described as follows: the invention provides a flow cytometry sample test tube treatment device, which comprises a main body 1 with an isolation cavity, wherein a sampling box 2 is arranged in the isolation cavity, the sampling box 2 is provided with a sampling cavity 201 for accommodating a sample moving device 6 and a manipulator 9, the sample moving device 6 firstly moves out of the sampling box 2 so as to allow workers to place test tubes 8 on the sample moving device 6, then the sample moving device 6 drives the test tubes 8 to take in the isolation cavity and align one test tube 8 with the manipulator 9, a suction device 11 is also arranged in the isolation cavity and positioned right above the manipulator 9, the manipulator 9 grabs the aligned test tubes 8 and drives the test tubes 8 to move upwards along the vertical direction, a sleeve 116 of the suction device 11 and part of a connecting column 114 extend into the test tubes 8 from the opening of the test tubes 8 and seal the opening of the test tubes 8, a suction tube 118 and a cleaning tube 117 of the suction device 11 extend into the test tubes 8, a discharge hole 113 positioned on the peripheral wall of the connecting column 114 also extend into the test tubes 8, the suction tube 118, the cleaning tube 117 and the discharge tube 119 communicated with the discharge tube 113 sequentially move to the test tubes 8, and then the sample tube 118 is sucked into the test tubes 8, and the waste liquid is pumped from the test tubes are pumped and the test tubes are pumped out of the test tubes 8, and the waste liquid is pumped into the test tubes are pumped and pumped into the test tubes 8, and then the test tubes are pumped and the waste liquid is pumped into the test tubes and the test tubes are pumped and completely and pumped.
From the above description, the beneficial effects of the invention are as follows: the flow cytometer sample tube treatment device provided by the invention can timely recover and treat waste liquid and waste gas generated after sample introduction, and clean the test tube 8, so as to avoid experimental environment pollution caused by volatilization of residual sample in the test tube 8 into the air, and prevent operators or experimental instruments from being adversely affected.
Further, an isolation cover 101 is rotatably connected to the main body 1, and the isolation cover 101 covers the opening of the isolation cavity.
As can be seen from the above description, the isolation cover 101 can be turned to open or close the isolation cavity on the main body 1, which is simple to operate and facilitates the placement of the test tube 8 on the sample moving device 6.
Further, the sampling box 2 further has a sterilizing chamber 202, a slidable isolation door 15 is disposed between the sampling chamber 201 and the sterilizing chamber 202, and a sterilizing lamp 12 is disposed on an inner peripheral wall of the sterilizing chamber 202.
As can be seen from the above description, the disinfection chamber 202 is provided in the sampling box 2 to disinfect the test tube 8 before sample injection, so as to kill other microorganisms carried by the test tube 8 when the test tube enters the isolation chamber, and prevent the sample from being polluted by the microorganisms in the air.
Further, the sample moving device 6 includes a boss 602 and a driving member for driving the boss 602 to move, the boss 602 is movably disposed along a length direction of the sampling cavity 201, a plurality of placement grooves 601 are further disposed on the boss 602, and the placement grooves 601 are disposed at intervals along a moving direction of the boss 602.
As can be seen from the above description, the sample moving device 6 includes a boss 602 for placing the test tube 8 and a driving member for driving the boss 602 to move, and a plurality of placing grooves 601 are provided on the boss 602 to accommodate the test tube 8, so that the test tube 8 is stably placed on the boss 602, and the test tube 8 is prevented from falling off from the image table during the movement of the boss 602.
Further, the suction device 11 further includes an inflator 1151, and a plurality of inflation sleeves 115 are disposed on the outer peripheral wall of the connecting post 114 at intervals, and the inflator 1151 is respectively communicated with the inflation sleeves 115.
As is apparent from the above description, a plurality of air-filling sleeves 115 are provided on the outer peripheral wall of the connecting column 114, and after the connecting column 114 is partially inserted into the test tube 8, the air-filling sleeves 115 are inflated by the air pump to expand the air-filling sleeves 115 and abut against the inner wall of the test tube 8, so as to seal the test tube 8, and the flow cytometer sample test tube processing apparatus can be adapted to the test tubes 8 with various calibers.
Further, an inner concave groove is formed in the peripheral wall of the connecting post 114, and the inflatable sleeve 115 is embedded in the groove.
From the above description, the insertion of the air-filling sleeve 115 into the groove on the outer peripheral wall of the connecting post 114 can prevent the connecting post 114 from being inserted into the test tube 8, so that the air-filling sleeve 115 rubs against the inner wall of the test tube 8, and the air-filling sleeve 115 is prevented from being displaced or worn.
Further, the flow cytometer further comprises a feed pump 120, wherein the input end of the feed pump 120 is communicated with the suction pipe 118, and the output end of the feed pump 120 is communicated with the flow chamber 100 of the flow cytometer.
As can be seen from the above description, the sample is pumped from the tube 8 into the flow cell 100 of the flow cytometer by the feed pump 120 through the aspiration tube 118, where the sample is combined with the sheath fluid to achieve autoloading.
Further, the device also comprises a liquid storage tank 5 and a cleaning pump 121, wherein the input end of the cleaning pump 121 is communicated with the liquid storage tank 5, and the output end of the cleaning pump 121 is communicated with the cleaning pipe 117.
As is apparent from the above description, the cleaning agent in the liquid storage tank 5 is pumped into the test tube 8 by the cleaning pump 121 through the cleaning tube 117 to clean the test tube 8.
Further, a waste bin 4 and a waste pump 122 are also included, an input end of the waste pump 122 is communicated with the discharge pipe 119, and an output end of the waste pump 122 is communicated with the waste bin 4.
As is apparent from the above description, the waste liquid and the exhaust gas in the test tube 8 are pumped into the waste bin 4 through the exhaust pipe 119 by the waste pump 122 to concentrate the waste liquid and the exhaust gas for easy disposal.
Further, a controller 3 is further disposed in the main body 1, the controller 3 is electrically connected with the sample moving device 6, the manipulator 9 and the suction device 11, the controller 3 is used for implementing the following steps,
the sample moving device 6 partially extends out of the sampling box 2, and a test tube 8 containing a sample is placed on the sample moving device 6;
the sample moving means 6 retract inside the sampling cartridge 2 and align one of the test tubes 8 with the manipulator 9;
the manipulator 9 clamps the test tube 8 and drives the test tube 8 to move upwards along the vertical direction, so that the suction tube 118, the cleaning tube 117, the sleeve 116 and part of the connecting column 114 extend into the test tube 8;
the suction pipe 118 sucks the sample in the test tube 8 for sample injection;
after the sample injection is completed, the cleaning tube 117 injects cleaning liquid into the test tube 8 for cleaning;
after the cleaning is completed, the waste liquid and the waste gas in the test tube 8 are collected by the discharge tube 119 for waste recovery;
after the waste recovery is completed, the manipulator 9 drives the test tube 8 to move downwards along the vertical direction and returns the test tube 8 to the sample moving device 6, the sample moving device 6 moves to align another test tube 8 containing the sample with the manipulator 9, and the sample injection step, the cleaning step and the waste recovery step are repeated until all the test tubes 8 are subjected to sample injection.
As can be seen from the above description, the controller 3 controls the sample moving device 6, the manipulator 9 and the suction device 11 to operate according to preset steps, so as to automatically implement the steps of sample injection, tube 8 cleaning, waste recovery and the like, so that the flow cytometer sample tube processing device is convenient to use.
Example 1
Referring to fig. 1 to 9, a first embodiment of the present invention is as follows: a flow cytometer sample tube processing device is used for realizing automatic sample injection of a flow cytometer, and cleaning and waste recovery are carried out on a sample injected test tube 8.
As shown in fig. 1 and 2, the flow cytometer sample tube processing apparatus includes a main body 1, the main body 1 has a receiving cavity, and the receiving cavity is provided therein with a sampling box 2 for receiving a tube 8, a sample moving device 6 for moving the tube 8 in a horizontal direction, a manipulator 9 for moving the tube 8 in a vertical direction, and a suction device 11 for sucking the sample in the tube 8 and cleaning the tube 8.
In order to prevent the components in the accommodating cavity from being exposed in a large area, the main body 1 is rotatably connected with the isolation cover 101, the isolation cover 101 covers the opening of the isolation cavity, the isolation cover 101 can be manually or electrically rotated on the main body 1 to realize the opening and closing of the isolation cover 101, and the isolation cover 101 is closed after the test tube 8 containing the sample is placed in the isolation cavity to prevent dust and other impurities in the environment from falling into the sample.
Please combine fig. 2, fig. 3, fig. 7 and fig. 8, the sampling box 2 has a sampling cavity 201, the opening setting of sampling cavity 201 is in sampling box 2 is close to one side of the opening of isolation cavity, sample mobile device 6 sets up in the sampling cavity 201, sample mobile device 6 includes along the length direction slidable boss 602 of sampling cavity 201 and drive boss 602 gliding actuating member, be equipped with a plurality of standing grooves 601 on the boss 602, the standing grooves 601 is followed the slip direction interval setting of boss 602, actuating member includes fixing servo cylinder 13 in the sampling cavity 201 and sets up servo cylinder 13 output connecting rod 14, connecting rod 14 is kept away from servo cylinder 13's one end with boss 602 links to each other, servo cylinder 13 drives connecting rod 14 stretches out and draws back in order to drive boss 602 removes, accessible manual work or current automation equipment is to place sample tube 8 in the standing groove 601 in, wait for test tube 8 place in the standing groove is placed in servo cylinder 13 and is moved to test tube 1 in the main part 1 is moved to the servo cylinder 13 after placing tube 8 in place in the standing groove 602.
Preferably, the side of the sampling box 2 far away from the opening of the isolation cavity is further provided with a disinfection cavity 202, the inner peripheral wall of the disinfection cavity 202 is provided with a disinfection lamp 12 capable of emitting ultraviolet rays, the disinfection cavity 202 is communicated with the sampling cavity 201, the sampling box 2 is internally provided with an isolation door 15 for separating the sampling cavity 201 from the disinfection cavity 202, the isolation door 15 is slidably arranged in the sampling cavity 201, when the boss 602 moves outwards of the sampling box 2, the isolation door 15 is opened, after the boss 602 is filled with the test tube 8, the servo cylinder 13 drives the boss 602 to move into the disinfection cavity 202 and the isolation door 15 is closed, and at the moment, the disinfection lamp 12 is started and irradiates the test tube 8 on the boss 602 to kill microorganisms attached to the test tube 8 and the boss 602, so that the test tube is prevented from being influenced by the microorganism pollution in the environment.
As shown in fig. 2 and 3, the manipulator 9 is disposed on the inner peripheral wall of the sampling cavity 201, the manipulator 9 is located directly above the boss 602 and one of the test tubes 8 is aligned with the manipulator 9 after the boss 602 moves into the sampling cavity 201, a suction box 7 is further disposed directly above the manipulator 9, and the suction device 11 is disposed in the suction box 7.
Specifically, the manipulator 9 includes two gripper jaws that set up relatively, be equipped with the first sensor 10 that is used for discernment test tube 8 position on the gripper jaw, after the position of test tube 8 is obtained to first sensor 10, two gripper jaw presss from both sides tight test tube 8 and drives test tube 8 and rise in the vertical direction in order to stretch into the opening part of test tube 8 and draw in the suction box 7 supplies suction means 11 to draw, after the sample in the test tube 8 is absorbed by suction means 11, the gripper jaw drives test tube 8 along vertical direction downward movement and puts back test tube 8 standing groove 601, put back test tube 8 back the gripper jaw loosens test tube 8 and resets, at this moment sample mobile device 6 motion makes another test tube 8 that holds the sample with manipulator 9 aligns to realize continuous sampling.
Referring to fig. 3 to 6, the suction device 11 includes a suction nozzle, the suction nozzle has a mounting base 111, a connection post 114 and a sleeve 116 sequentially connected along a vertical direction, the sleeve 116 is disposed at one end of the connection post 114 near the manipulator 9, the suction device 11 further includes a cleaning tube 117, a suction tube 118 and a discharge tube 119, the cleaning tube 117 and the suction tube 118 respectively penetrate through the mounting base 111, the connection post 114 and the sleeve 116 along the vertical direction and extend toward the direction near the manipulator 9, and the discharge tube 119 extends into the connection post 114 and is provided with a discharge hole 113 communicated with the discharge tube 119 on the peripheral wall of the connection post 114.
In detail, when the manipulator 9 moves the test tube 8 in the vertical upward direction, the sleeve 116 and a part of the connecting post 114 sequentially extend into the test tube 8 and are attached to the inner peripheral wall of the test tube 8 to seal the test tube 8, at this time, the cleaning tube 117 and the suction tube 118 extend into the bottom of the test tube 8, and the discharge hole 113 is located inside the test tube 8. The suction pipe 118, the cleaning pipe 117 and the discharge pipe 119 run in sequence, the suction pipe 118 sucks the sample in the test tube 8 to sample, after the sample is introduced, the cleaning pipe 117 injects cleaning liquid into the test tube 8, the cleaning liquid cleans the test tube 8 from bottom to top in the test tube 8, after the cleaning, the discharge pipe 119 sucks waste liquid in the test tube 8 and waste gas discharged when the suction pipe 118 guides the sample, thereby realizing cleaning of the test tube 8 and waste collection, and preventing the sample and the waste gas remained in the test tube 8 from volatilizing into the air to pollute the experimental environment or cause adverse effects on operators.
As shown in fig. 1 and 9, a waste tank 4 and a liquid storage tank 5 are further arranged in the isolation cavity, the waste tank 4 is used for storing waste liquid and waste gas sucked by the discharge pipe 119, and a cleaning liquid is stored in the liquid storage tank 5 for cleaning the test tube 8. A feeding pump 120, a cleaning pump 121 and a waste pump 122 are further arranged in the main body 1, wherein the input end of the feeding pump 120 is communicated with the suction pipe 118, the output end of the feeding pump 120 is communicated with the flow chamber 100 of the flow cytometer, and the sample in the test tube 8 is pumped into the flow chamber 100 by the feeding pump 120 to be combined with sheath fluid; the input end of the cleaning pump 121 is communicated with the liquid storage tank 5, and the output end of the cleaning pump 121 is communicated with the cleaning pipe 117, so that the cleaning liquid in the liquid storage tank 5 is pumped into the test tube 8 to clean the end parts of the test tube 8 and the suction pipe 118; the input end of the waste pump 122 is communicated with the discharge pipe 119, and the output end of the waste pump 122 is communicated with the waste box 4, so that waste liquid and waste gas in the test tube 8 are pumped into the waste box 4 through the waste pump 122 for collection and storage.
Referring to fig. 5, 6 and 9, three air-filling sleeves 115 are disposed on the outer peripheral wall of the connecting post 114 at intervals, the air-filling sleeves 115 are annular and are disposed around the connecting post 114, an air pump 1151 is further disposed in the main body 1, the output end of the air pump 1151 is respectively communicated with the three air-filling sleeves 115, when the connecting post 114 is partially inserted into the test tube 8, the air pump 1151 inflates the air-filling sleeves 115 to expand the air-filling sleeves 115 and support the inner peripheral wall of the test tube 8, so as to ensure the air tightness of the matched suction nozzle and the test tube 8, ensure that the suction nozzle can be matched with the test tube 8 with different calibers, ensure sealing when the matched suction nozzle is used, and avoid the need of replacing the sleeve 116 with different sizes due to the different calibers of the used test tube 8, so that the flow cytometer sample processing device is convenient to use.
Preferably, the air-filling sleeve 115 is made of rubber, and the shape of the air-filling sleeve 115 is in a smooth arc shape, so that friction between the air-filling sleeve 115 and the inner wall of the test tube 8 is reduced, abrasion or rupture of the air-filling sleeve 115 is prevented, and the service life of the air-filling sleeve 115 is prolonged.
As shown in fig. 4, the mounting base 111 is further provided with a second sensor 112 for sensing the expansion degree of the inflation sleeve 115, the caliber of the test tube 8 is measured by the second sensor 112 to determine the expansion degree of the inflation sleeve 115, and then the inflation pump 1151 inflates the inflation sleeve 115, so as to prevent the inflation sleeve 115 from being excessively expanded to damage the test tube 8 or the inflation sleeve 115.
As shown in fig. 1 and 9, a controller 3 is further disposed in the isolation chamber, the controller 3 is electrically connected to the sample moving device 6, the sterilizing lamp 12, the isolation door 15, the first sensor 10, the manipulator 9, the suction device 11, and the second sensor 112, and the controller 3 is configured to implement the following steps:
s1, controlling the isolation door 15 to be opened, starting the servo electric cylinder 13 to drive the boss 602 to extend out of the sampling cavity 201, and placing a test tube 8 containing a sample on the boss 602;
s2, starting the servo electric cylinder 13 to drive the boss 602 to retract into the sampling cavity 201 and move to the disinfection cavity 202, controlling the isolation door 15 to be closed, starting the disinfection lamp 12 to disinfect and sterilize the test tube 8 and the boss 602, and closing the disinfection lamp 12 after disinfection is completed;
s3, starting the servo cylinder 13 to drive the boss 602 to move and judging the position of the test tubes 8 through the first sensor 10, so that one test tube 8 is aligned with the manipulator 9;
s4, driving the manipulator 9 to clamp the test tube 8, wherein the manipulator 9 drives the test tube 8 to move upwards along the vertical direction, so that the suction tube 118, the cleaning tube 117, the sleeve 116 and part of the connecting column 114 extend into the test tube 8;
s5, judging the caliber of the test tube 8 and the inflation degree of the inflation sleeve 115 through the second sensor 112, starting the inflator pump 1151 to inflate the inflation sleeve 115 so as to expand the inflation sleeve 115 and seal the opening of the test tube 8;
s6, starting the feeding pump 120, and leading the sample in the test tube 8 to enter the flow chamber 100 by the suction pipe 118 to be converged with sheath fluid so as to realize sample injection;
s7, after sample injection is completed, starting the cleaning pump 121, and injecting the cleaning liquid in the liquid storage tank 5 into the test tube 8 through the cleaning tube 117 for cleaning;
s8, after the cleaning is finished, starting the waste pump 122, and collecting waste liquid and waste gas in the test tube 8 to the waste bin 4 through the discharge tube 119 for waste recovery;
and S9, after the waste recovery is completed, the manipulator 9 drives the test tube 8 to move downwards along the vertical direction and put the test tube 8 back on the boss 602, the servo cylinder 13 is started to drive the boss 602 to move so that the other test tube 8 containing the sample is aligned with the manipulator 9, and the steps S3 to S8 are repeated until all the test tubes 8 are subjected to sample injection.
Example two
Referring to fig. 10, the second embodiment of the present invention provides another technical solution for the structure of the connecting post 114 based on the first embodiment, and the difference between the second embodiment and the first embodiment is only that the structure of the connecting post 114 is different.
As shown in fig. 10, a plurality of grooves are formed in the outer peripheral wall of the connecting column 114, the air-filling sleeve 115 is embedded into the grooves, the air-filling sleeve 115 is accommodated in the grooves when the air-filling sleeve 115 is in an uninflated state, and after the connecting column 114 is partially inserted into the test tube, the air-filling sleeve 115 is inflated and extends out of the grooves to abut against the inner wall of the test tube, so that the test tube is sealed. The groove is formed in the outer peripheral wall of the connecting post 114 to accommodate the inflation sleeve 115 in an uninflated state, so that displacement or abrasion of the inflation sleeve 115 due to friction between the inflation sleeve 115 and a test tube when the connecting post 114 stretches into the test tube can be avoided, and the service life of the inflation sleeve 115 is further prolonged.
In summary, the flow cytometer sample tube processing device provided by the invention adopts the controller to control the components such as the sample moving device, the mechanical arm, the suction device and the like to operate according to the preset steps, so that automatic sample injection, tube cleaning and waste recovery are realized, the sample-containing test tube is cleaned in time after sample injection is completed, and the waste water and waste gas generated by sample injection are collected and stored for centralized processing, thereby effectively preventing the waste material from volatilizing into the air to cause experimental environment pollution and avoiding adverse effects on operators or experimental instruments; the flow cytometer sample tube processing device comprises a main body and an isolation cover rotatably arranged on the main body, wherein an isolation cavity of the main body can be conveniently opened or closed by rotating the isolation cover so as to reduce the contact between a sample and the external environment in the sample injection process; a disinfection cavity and a disinfection lamp are also arranged in the sampling box of the flow cytometry sample tube processing device, so that the sample is automatically disinfected and sterilized before sample injection, and the sample is prevented from being polluted by microorganisms in the air; the suction device of the flow cytometry sample tube processing device adopts the suction nozzle with the air charging sleeve, and the opening of the test tube is sealed by the air charging sleeve air charging expansion, so that the test tubes with various calibers can be matched with the suction device, the flow cytometry sample tube processing device is convenient to use, the suction nozzle is not required to be replaced according to the caliber of the test tube, and the groove for accommodating the air charging sleeve is formed in the connecting column of the suction nozzle, so that the air charging sleeve is prevented from being offset or worn due to friction between the air charging sleeve and the inner wall of the test tube, and the service life of the suction nozzle is effectively prolonged.
The foregoing description is only illustrative of the present invention and is not intended to limit the scope of the invention, and all equivalent changes made by the specification and drawings of the present invention, or direct or indirect application in the relevant art, are included in the scope of the present invention.
Claims (9)
1. Flow cytometer sample tube processing apparatus, characterized by comprising:
a body having an isolation cavity;
the sampling box is arranged in the isolation cavity and is provided with a sampling cavity;
the sample moving device is arranged in the sampling cavity;
the manipulator is arranged in the sampling cavity and is matched with the sample moving device;
the suction device is arranged in the isolation cavity and comprises a connecting column and a sleeve which are connected, the sleeve is positioned right above the manipulator, one side of the sleeve, which is close to the manipulator, is provided with a suction pipe and a cleaning pipe, the suction device also comprises a discharge pipe, and the peripheral wall of the connecting column is provided with a discharge hole communicated with the discharge pipe;
the suction device further comprises an inflator pump, a plurality of inflation sleeves are arranged on the peripheral wall of the connecting column at intervals, and the inflator pump is respectively communicated with the inflation sleeves.
2. The flow cytometer sample tube processing device as described in claim 1, wherein: the main body is rotatably connected with an isolation cover, and the isolation cover covers the opening of the isolation cavity.
3. The flow cytometer sample tube processing device as described in claim 1, wherein: the sampling box is also provided with a disinfection cavity, a slidable isolation door is arranged between the sampling cavity and the disinfection cavity, and a disinfection lamp is arranged on the inner peripheral wall of the disinfection cavity.
4. The flow cytometer sample tube processing device as described in claim 1, wherein: the sample moving device comprises a boss and a driving piece for driving the boss to move, the boss is movably arranged along the length direction of the sampling cavity, a plurality of placing grooves are further formed in the boss, and the placing grooves are arranged at intervals along the moving direction of the boss.
5. The flow cytometer sample tube processing device as described in claim 1, wherein: the periphery wall of the connecting column is provided with a concave groove, and the inflatable sleeve is embedded into the groove.
6. The flow cytometer sample tube processing device as described in claim 1, wherein: the flow cytometer comprises a flow cytometer body, a suction pipe, a feeding pump, a flow cytometer chamber and a suction pipe, wherein the suction pipe is arranged at the bottom of the flow cytometer body, the suction pipe is arranged at the top of the flow cytometer body, the suction pipe is arranged at the bottom of the flow cytometer body, the feeding pump is arranged at the top of the flow cytometer body, the input end of the feeding pump is communicated with the suction pipe.
7. The flow cytometer sample tube processing device as described in claim 1, wherein: the cleaning device further comprises a liquid storage tank and a cleaning pump, wherein the input end of the cleaning pump is communicated with the liquid storage tank, and the output end of the cleaning pump is communicated with the cleaning pipe.
8. The flow cytometer sample tube processing device as described in claim 1, wherein: still include waste bin and waste pump, the input of waste pump with row material pipe intercommunication, the output of waste pump with waste bin intercommunication.
9. The flow cytometer sample tube processing device as described in claim 1, wherein: a controller is also arranged in the main body and is respectively and electrically connected with the sample moving device, the manipulator and the suction device, the controller is used for realizing the following steps,
the sample moving device part extends out of the sampling box, and a test tube containing a sample is placed on the sample moving device;
the sample moving device retreats into the sampling box and aligns one test tube with the manipulator;
the manipulator clamps the test tube and drives the test tube to move upwards along the vertical direction, so that the suction tube, the cleaning tube, the sleeve and part of the connecting column extend into the test tube;
the suction pipe sucks a sample in the test tube for sample injection;
after sample injection is completed, the cleaning tube injects cleaning liquid into the test tube for cleaning;
after the cleaning is finished, the waste liquid and the waste gas in the test tube are collected by the discharge tube for waste recovery;
after the waste recovery is completed, the manipulator drives the test tube to move downwards along the vertical direction and returns the test tube to the sample moving device, the sample moving device moves to align another test tube containing the sample with the manipulator, and the sample injection step, the cleaning step and the waste recovery step are repeated until all the test tubes are subjected to sample injection.
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