CN206906212U - A kind of cell sorting device of FCM analysis - Google Patents
A kind of cell sorting device of FCM analysis Download PDFInfo
- Publication number
- CN206906212U CN206906212U CN201720856375.7U CN201720856375U CN206906212U CN 206906212 U CN206906212 U CN 206906212U CN 201720856375 U CN201720856375 U CN 201720856375U CN 206906212 U CN206906212 U CN 206906212U
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- Prior art keywords
- test tube
- spring
- groove
- nozzle
- cabinet
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Abstract
The utility model discloses a kind of cell sorting device of FCM analysis, including flow chamber, the flow chamber includes sample cell, nozzle and sheath liquid pipe, and nozzle is located at the lower section of sample cell, the outer wall of the flow chamber is socketed with piezo-electric crystal, the side of the flow chamber is provided with cabinet by fastening bolt, and the middle part of the cabinet one side is separately installed with argon ion laser and photomultiplier by fastening bolt.The utility model is provided with ultraviolet lamp bar in the both sides of cabinet, without using when open ultraviolet lamp bar device is sterilized, it ensure that the quality of cell sorting, set in bottom fluted, several collection test tubes and waste liquid test tube are placed in groove respectively, the side of groove is provided with spring, take out after completely collecting test tube and waste liquid test tube, empty collection test tube and waste liquid test tube can be under the action of the spring, appliance for releasing single makes the replacing of collection test tube and waste liquid test tube more convenient to suitably position is received.
Description
Technical field
It the utility model is related to cell sorting device technical field, more particularly to a kind of cell sorting of FCM analysis
Device.
Background technology
Flow cytometry sorting devices are the devices that cell is automatically analyzed and sorted.It can quickly measure, store,
The characteristic parameter in terms of a series of important biophysics of cell dispersion to float on a liquid, biochemistry is shown, and can
Specified cell subsets is therefrom sorted out with the parameter ranges according to pre-selection.But existing flow cytometry sorting devices are deposited
Sterilizing is inadequate before the use, causes the contaminated problem of cell sub-elected, and changed in sorting and collect test tube and waste liquid
The problem of test tube inconvenience.
Utility model content
The purpose of this utility model is and a kind of fluidic cell inspection proposed in order to solve shortcoming present in prior art
The cell sorting device of survey.
To achieve these goals, the utility model employs following technical scheme：
A kind of cell sorting device of FCM analysis, including flow chamber, the flow chamber include sample cell, nozzle
With sheath liquid pipe, and nozzle is located at the lower section of sample cell, and the outer wall of the flow chamber is socketed with piezo-electric crystal, and the one of the flow chamber
Side is provided with cabinet by fastening bolt, and the middle part of the cabinet one side is separately installed with argon laser by fastening bolt
Device and photomultiplier, and argon ion laser and photomultiplier are located at the both sides of nozzle respectively, the cabinet one side
Bottom is provided with two symmetrical deflecting plates by fastening bolt, and the bottom of the cabinet is provided with bottom by fastening bolt
Seat, the top of the base offer the first groove, the second groove and the 3rd groove respectively, and the first groove, the second groove and
The side of 3rd groove is connected with the first spring, second spring and the 3rd spring respectively, and five are placed with first groove
First collects test tube, is placed with five waste liquid test tubes in second groove, five second receipts are placed with the 3rd groove
Collect test tube, the both sides of the cabinet one side are provided with ultraviolet lamp bar by fastening bolt.
Preferably, one end of first spring, second spring and the 3rd spring is socketed with rubber stopper, and rubber stopper is remote
Test tube is collected with the first collection test tube, waste liquid test tube, second in side from the first spring, second spring and the 3rd spring respectively
Outer wall is in close contact.
Preferably, the deflecting plates is located at the both sides of the underface of nozzle.
Preferably, described first test tube and the second collection test tube are collected respectively positioned at the underface of two deflecting plates, and given up
Liquid test tube is located at the underface of nozzle.
Preferably, the sample cell, nozzle and sheath liquid pipe are optical glass material, and sample cell, nozzle and sheath liquid pipe
Be formed in one structure.
Preferably, the cabinet and base are plastic material.
The beneficial effects of the utility model are：
1. be provided with ultraviolet lamp bar in the both sides of cabinet, without using when open ultraviolet lamp bar device carried out
Sterilizing, ensure that the quality of cell sorting.
2. setting is fluted in bottom, several collection test tubes are placed in groove respectively and waste liquid test tube, the side of groove are set
There is spring, take out after completely collecting test tube and waste liquid test tube, empty collection test tube and waste liquid test tube can under the action of the spring, certainly
Move to suitably position is received, make the replacing of collection test tube and waste liquid test tube more convenient.
Brief description of the drawings
Fig. 1 be the utility model proposes a kind of FCM analysis cell sorting device structural representation；
Fig. 2 be the utility model proposes a kind of FCM analysis cell sorting device structural representation.
In figure：1 first collects test tube, 2 first grooves, 3 second grooves, 4 waste liquid test tubes, 5 the 3rd grooves, 6 second collections
Test tube, 7 bases, 8 deflecting plates, 9 photomultipliers, 10 flow chambers, 11 ultraviolet lamp bars, 12 piezo-electric crystals, 13 cabinets, 14 samples
Pipe, 15 argon ion lasers, 16 nozzles, 17 first springs, 18 second springs, 19 the 3rd springs.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.
Reference picture 1-2, a kind of cell sorting device of FCM analysis, including flow chamber 10, flow chamber 10 include
Sample cell 14, nozzle 16 and sheath liquid pipe, and nozzle 16 is located at the lower section of sample cell 14, the outer wall of flow chamber 10 is socketed with piezo crystals
Body 12, the side of flow chamber 10 are provided with cabinet 13 by fastening bolt, and the middle part of the one side of cabinet 13 passes through fastening bolt point
Argon ion laser 15 and photomultiplier 9 are not installed, and argon ion laser 15 and photomultiplier 9 are located at nozzle respectively
16 both sides, the bottom of the one side of cabinet 13 are provided with two symmetrical deflecting plates 8, the bottom of cabinet 13 by fastening bolt
Base 7 is provided with by fastening bolt, the top of base 7 offers the first groove 2, the second groove 3 and the 3rd groove 5 respectively,
And the side of first groove 2, the second groove 3 and the 3rd groove 5 is connected with the first spring 17, the bullet of second spring 18 and the 3rd respectively
Spring 19, five first collection test tubes 1 are placed with the first groove 2, are placed with five waste liquid test tubes 4 in the second groove 3, the 3rd is recessed
Five second collection test tubes 6 are placed with groove 5, the both sides of the one side of cabinet 13 are provided with ultraviolet lamp bar by fastening bolt
11。
In the utility model, the first spring 17, one end of the spring 19 of second spring 18 and the 3rd are socketed with rubber stopper, and
Test tube 1, waste liquid test tube are collected with first in side of the rubber stopper away from the first spring 17, the spring 19 of second spring 18 and the 3rd respectively
4th, the second outer wall for collecting test tube 6 is in close contact, and deflecting plates 8 is located at the both sides of the underface of nozzle 16, and first collects the He of test tube 1
Second collects test tube 6 respectively positioned at the underface of two deflecting plates 8, and waste liquid test tube 4 is located at the underface of nozzle 16, sample cell
14th, nozzle 16 and sheath liquid pipe are optical glass material, and sample cell 14, nozzle 16 and sheath liquid pipe are formed in one structure, cabinet
Body 13 and base 7 are plastic material.
Operation principle：The sample suspension that need to be sorted is put into sample cell 14, sample suspension projects out of sample cell 14, sample
Product project after being surrounded by the sheath fluid that sheath liquid pipe sprays from nozzle 16, the laser irradiation through argon ion laser 15 at nozzle 16,
Sample upon laser irradiation is measured by photomultiplier 9, and sample separately flows into first after being sorted when deflecting plates 8
Collect test tube 1, second and collect test tube 6 and waste liquid test tube 4, when positioned at the first groove 2, the second groove 3 and the outermost of the 3rd groove 5
First collection test tube 1, waste liquid test tube 4 and second collect test tube 6 fill and take out, empty one collect test tube 1, waste liquid test tube 4
With second collect test tube 6 respectively in the presence of the first spring 17, the spring 19 of second spring 18 and the 3rd bullet to groove outermost
Side.
It is described above, the only preferable embodiment of the utility model, but the scope of protection of the utility model is not
This is confined to, any one skilled in the art is in the technical scope that the utility model discloses, according to this practicality
New technical scheme and its utility model design are subject to equivalent substitution or change, should all cover in protection model of the present utility model
Within enclosing.
Claims (6)
1. a kind of cell sorting device of FCM analysis, including flow chamber (10), it is characterised in that the flow chamber (10)
Include sample cell (14), nozzle (16) and sheath liquid pipe, and nozzle (16) is located at the lower section of sample cell (14), the flow chamber
(10) outer wall is socketed with piezo-electric crystal (12), and the side of the flow chamber (10) is provided with cabinet (13) by fastening bolt,
The middle part of cabinet (13) one side is separately installed with argon ion laser (15) and photomultiplier by fastening bolt
(9), and argon ion laser (15) and photomultiplier (9) are located at the both sides of nozzle (16), cabinet (13) side respectively
The bottom in face is provided with two symmetrical deflecting plates (8) by fastening bolt, and the bottom of the cabinet (13) is by fastening spiral shell
Bolt is provided with base (7), and the top of the base (7) offers the first groove (2), the second groove (3) and the 3rd groove respectively
(5), and the side of the first groove (2), the second groove (3) and the 3rd groove (5) is connected with the first spring (17), the second bullet respectively
Spring (18) and the 3rd spring (19), first groove (2) is interior to be placed with five first collection test tubes (1), second groove
(3) five waste liquid test tubes (4) are placed with, five second are placed with the 3rd groove (5) and collects test tube (6), the cabinet
The both sides of body (13) one side are provided with ultraviolet lamp bar (11) by fastening bolt.
A kind of 2. cell sorting device of FCM analysis according to claim 1, it is characterised in that first bullet
One end of spring (17), second spring (18) and the 3rd spring (19) is socketed with rubber stopper, and rubber stopper is away from the first spring
(17), test tube (1), waste liquid test tube (4), the second receipts are collected in the side of second spring (18) and the 3rd spring (19) with first respectively
The outer wall for collecting test tube (6) is in close contact.
A kind of 3. cell sorting device of FCM analysis according to claim 1, it is characterised in that the deflecting plates
(8) positioned at the both sides of the underface of nozzle (16).
4. the cell sorting device of a kind of FCM analysis according to claim 1, it is characterised in that described first receives
Collect test tube (1) and second and collect test tube (6) respectively positioned at the underface of two deflecting plates (8), and waste liquid test tube (4) is located at nozzle
(16) underface.
A kind of 5. cell sorting device of FCM analysis according to claim 1, it is characterised in that the sample cell
(14), nozzle (16) and sheath liquid pipe are optical glass material, and sample cell (14), nozzle (16) and sheath liquid pipe are formed in one
Structure.
A kind of 6. cell sorting device of FCM analysis according to claim 1, it is characterised in that the cabinet
(13) and base (7) is plastic material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720856375.7U CN206906212U (en) | 2017-07-14 | 2017-07-14 | A kind of cell sorting device of FCM analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720856375.7U CN206906212U (en) | 2017-07-14 | 2017-07-14 | A kind of cell sorting device of FCM analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206906212U true CN206906212U (en) | 2018-01-19 |
Family
ID=61313032
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720856375.7U Expired - Fee Related CN206906212U (en) | 2017-07-14 | 2017-07-14 | A kind of cell sorting device of FCM analysis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206906212U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108801883A (en) * | 2018-04-19 | 2018-11-13 | 深圳市趣方科技有限公司 | A kind of small suspended particulate flowing optical detection apparatus, testing agency and detection method |
| CN113109583A (en) * | 2021-04-13 | 2021-07-13 | 亚能生物技术（深圳）有限公司 | Sample test tube processing device of flow cytometer |
| CN114923840A (en) * | 2022-07-19 | 2022-08-19 | 瑞铂生物(无锡)有限公司 | Cell sorting device, portable flow cytometer and use method thereof |
-
2017
- 2017-07-14 CN CN201720856375.7U patent/CN206906212U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108801883A (en) * | 2018-04-19 | 2018-11-13 | 深圳市趣方科技有限公司 | A kind of small suspended particulate flowing optical detection apparatus, testing agency and detection method |
| CN113109583A (en) * | 2021-04-13 | 2021-07-13 | 亚能生物技术（深圳）有限公司 | Sample test tube processing device of flow cytometer |
| CN113109583B (en) * | 2021-04-13 | 2024-02-13 | 亚能生物技术（深圳）有限公司 | Flow cytometer sample test tube processing device |
| CN114923840A (en) * | 2022-07-19 | 2022-08-19 | 瑞铂生物(无锡)有限公司 | Cell sorting device, portable flow cytometer and use method thereof |
| CN114923840B (en) * | 2022-07-19 | 2022-10-21 | 瑞铂生物(无锡)有限公司 | Cell sorting device, portable flow cytometer and use method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180119 Termination date: 20190714 |