CN204630897U - FCM analysis liquid-way system - Google Patents

FCM analysis liquid-way system Download PDF

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Publication number
CN204630897U
CN204630897U CN201520368054.3U CN201520368054U CN204630897U CN 204630897 U CN204630897 U CN 204630897U CN 201520368054 U CN201520368054 U CN 201520368054U CN 204630897 U CN204630897 U CN 204630897U
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reaction tank
sample
dilution
pressure
hemolytic agent
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谭玉华
秦军芳
张炎
梁铁柱
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Exxon Guangzhou Bio Tech Ltd
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Exxon Guangzhou Bio Tech Ltd
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Abstract

The utility model relates to blood of human body detection technique field. and FCM analysis liquid-way system comprises dilution loading system and drives dilution syringe by motor, connects dilution bucket absorption dilution and is injected into flow chamber, reaction tank and sampling needle cleaning device; Hemolysin and blood sample loading system drive hemolytic agent syringe by motor, draw hemolytic agent and are injected into reaction tank, and the collection of sample injections device distributes blood sample and is injected into reaction tank; Pressure system sets up positive/negative-pressure by membrane pump Bonding pressure room, and pump drainage sampling needle cleaning device waste liquid, is drawn onto flow chamber sample inlet by sample in reaction tank, to reaction tank blow stir and emptying, extraction dilution to reaction tank.The utility model decreases reaction tank quantity, simplifies system architecture and complexity, facilitates machine to realize miniaturization and cost degradation.

Description

FCM analysis liquid-way system
Technical field
The utility model relates to blood of human body detection technique field, particularly a kind of FCM analysis liquid-way system and FCM analysis method.
Background technology
The liquid-way system of differential hematology analyzer in the market is mainly made up of impedance channel module and DIFF channel module, wherein impedance channel module comprises WBC/HGB counting chamber and RBC/PLT counting chamber and associated ping system thereof, DIFF channel module comprises DIFF incubation reaction pond and flow chamber assembly and associated ping system thereof, also has blood sampling point blood module in addition, discharging of waste liquid cleaning module, positive/negative-pressure builds die block.These modules are all relatively independent, need syringe corresponding with it and motor thereof, valve, pump or source of the gas and stable-pressure device, and fluid path device number of components is many, and systematic comparison is complicated, and failure risk is larger, and cost compare is high, can not meet the demand of basic unit user.Number of patent application CN201410173070.7) be that sheath streamer emitter and dilution syringe function merge into a dilution syringe, sample streamer emitter and sampling syringe function merge into a sample injections device, malleation builds pressing system and discharging of waste liquid system merges, dilution syringe, sample injections device, hemolytic agent syringe shares a motor, malleation builds pressing system, the same motor of discharging of waste liquid system drives, all syringes are shared a motor drive, affect the dirigibility of sequential control, affect system test speed, without pressure sensing unit in syringe main line, high pressure is easily caused to collapse pipe as there being blocking in pipeline, counting chamber impedance counting does not have after-bay cleaning part, very easily affect the result of next measurement, flow chamber outlet is directly connected with negative-pressure cup, if impedance counting and sheath stream easily affect the stability of impedance counting negative pressure simultaneously, count results is caused to be forbidden, can only serial perform, impact is tested the speed.The emptying shared three-way solenoid valve of sampling needle outer wall washing removal waste fluid valve and negative pressure chamber, this valve can not be switched during negative pressure chamber's pressurize counting and wash pin outer wall, can cause pressure surge, and cleaning needle outer wall passage and reaction tank emptying pipe all do not have filtration unit, increase the stifled valve probability of this common valve.
Utility model content
The purpose of this utility model is to provide a kind of low cost, high-level efficiency realizes blood cell five and classifies and the compact FCM analysis liquid-way system that counts and FCM analysis method.
The purpose of this utility model is achieved through the following channels:
FCM analysis liquid-way system, comprises dilution loading system, hemolysin and blood sample loading system and pressure system;
Described dilution loading system is after driving dilution syringe 7 to be connected in series liquid-pressure pick-up 8 by motor 6, be connected dilution bucket 3 by the road with solenoid valve and draw dilution, be injected into flow chamber 44, reaction tank 32 respectively and be injected into sampling needle cleaning device 20 and sampling needle 21 through sample injections device 24;
Described hemolysin and blood sample loading system drive the first hemolytic agent syringe 23, second hemolytic agent syringe 25, sample injections device 24 by motor 26, described first hemolytic agent syringe 23 and the second hemolytic agent syringe 25 are connected the first hemolytic agent bottle 2 and the second hemolytic agent bottle 1 by the road respectively with solenoid valve, draw hemolytic agent and be injected into reaction tank 32, described sample injections device 24 is connected sampling needle 21 collection distribution blood sample by the road and is injected into reaction tank 32 with solenoid valve;
Described pressure system sets up positive/negative-pressure with solenoid valve Bonding pressure room 38 by the road by membrane pump 41, described membrane pump 41 is connected sampling needle cleaning device 20 pump drainage waste liquid with solenoid valve and swab filtrator 22 by the road, described pressure chamber 38 by the road with solenoid valve and pinch valve 31 coupled reaction pond 32, sample is drawn onto flow chamber 44 sample inlet, described pressure chamber 38 by the road, solenoid valve and insulated chamber 33 coupled reaction pond 32, described pressure chamber 38 is connected dilution bucket 3 by the road with after the after-bay of solenoid valve coupled reaction pond 32.
Further: gas pressure sensor 36 is equipped with in described pressure chamber 38.
Further: point forebay and after-bay in described reaction tank 32, gem hole is housed between pond, front and back and does impedance counting, outside described reaction tank 32, be set with HGB test lamp assembly and thermostat.
Further: described first hemolytic agent syringe 23 and the second hemolytic agent syringe 25 are symmetrically distributed in sample injections device both sides.
Further: described insulated chamber 33 is built with filtration unit.
FCM analysis method adopts following steps:
The sample collection of the mat woven of fine bamboo strips one step, is communicated with sampling needle with sample injections device and draws blood sample, be injected into reaction tank;
The mat woven of fine bamboo strips two step sample incubation reacts, and adds the first hemolytic agent, then use two three-way electromagnetic valves of Bonding pressure room intermittent rapidly opened and closed to bubble in reaction tank with the first hemolytic agent syringe to reaction tank, and mixing blood sample and the first hemolytic agent, hatch after mixing.
The mat woven of fine bamboo strips three step sample prepares, and after sample incubation, is drawn into the standby sample pipeline of flow chamber sample inlet by pressure chamber's negative pressure in reaction tank by sample through pinch valve;
The mat woven of fine bamboo strips four step HGB measures, after standby sample is complete, in reaction tank, adds the second hemolytic agent with the second hemolytic agent syringe, then use two three-way electromagnetic valves of Bonding pressure room intermittent rapidly opened and closed to bubble in reaction tank, mixing blood sample and the second hemolytic agent, then measure HGB value by colourimetry;
The mat woven of fine bamboo strips five step dilution blood sample, cleaning reaction pond, with membrane pump through insulated chamber and the emptying reaction tank of two three-way electromagnetic valves, then dilution syringe rinses twice to liquid feeding in reaction tank, dilution is added to reaction tank afterwards with dilution syringe, sample injections device divides blood to enter reaction tank, then uses two three-way electromagnetic valves of Bonding pressure room intermittent rapidly opened and closed to bubble in reaction tank, mixing dilution and blood sample;
The mat woven of fine bamboo strips six step cell detection, dilution is injected to flow chamber inlet with dilution syringe, form sheath fluid, inject sample in standby sample pipeline with sample injections device to flow chamber sample inlet and form sample stream, two syringes are injected simultaneously, allow build the pressure in flow chamber stable after open flow chamber outlet valve, then sheath stream parcel sample flows to and carries out leucocyte five category measurement into flow chamber optical detecting parts, leukocytic concentration in blood sample is conversed according to the leukocytic quantity detected and the dilution ratio and volume detecting sample, open two three-way electromagnetic valves that pressure chamber is connected with reaction tank after-bay simultaneously, by negative pressure, the sample mixed liquor of reaction tank forebay is sucked after-bay by gem hole and carry out impedance method counting RBC and PLT cell quantity, then RBC and PLT cell concentration in blood sample is conversed with detection diluting blood sample ratio and volume,
The mat woven of fine bamboo strips seven step cleaning systems, after cell detection all terminates, in flow chamber and reaction tank, add dilution with dilution syringe and rinse, with membrane pump by emptying forebay, pressure chamber waste liquid, build negative pressure by after-bay to dilution bucket imbibition cleaning after-bay, after cleaning, all parts return to measurement standby condition.
Good effect of the present utility model: the utility model FCM analysis liquid-way system and FCM analysis method are all completed by dilution syringe because sheath stream is formed and adds dilution to reaction tank, sample stream in sample collection and sheath Flow Technique is formed and is completed by sample injections device, and the quantity which reducing syringe reduces cost.Due to multiple hemolytic agent syringe and the same drive and control of electric machine of sample injections device, decrease drive unit, thus whole system structure is more simple, facilitates machine to realize miniaturization and cost degradation.Due to dilution syringe relatively other syringes push away liquid often, it drives separately, makes sequential control more flexible, is conducive to improving systems axiol-ogy speed.Arrange liquid-pressure pick-up and carry out pressure monitoring to all liquid that pushes away, play the effect of overvoltage protection, prompting channel blockage, is convenient to fault handling and maintenance.Install swab filtrator in sampling needle cleaning device drain side additional and install the insulated chamber being with filter core in reaction tank lower end additional, can effectively filter foreign impurity bulky grain, prevent the emptying pipeline in blocking rear end, especially prevent membrane pump to build malleation and be connected in series three-way solenoid valve blocking and cause waste liquid arrange do not walk, the problem that pressure easily leaks.Because leukocyte differential count sample incubation and HGB measure, RBC/PLT cell count all uses same reaction tank, decreases reaction tank quantity, simplifies system architecture and complexity, facilitates machine to realize miniaturization and cost degradation.
Accompanying drawing explanation
Fig. 1 is the overvoltage protection control flow schematic diagram in the utility model
Fig. 2 is the utility model structural representation
In figure: 1. the first hemolytic agent bottle, 2. the second hemolytic agent bottle, 3. dilution bucket, 4. three-way connection, 5. first liang of three-way electromagnetic valve, 6. motor 7. dilution syringe, 8. liquid-pressure pick-up, 9. three-way connection, 10. the first three-way solenoid valve, 11. second three-way solenoid valves, 12. second liang of three-way electromagnetic valves, 13. the 3rd liang of three-way electromagnetic valves, 14. the 4th liang of three-way electromagnetic valves, 15. escape holes, 16. sheath streamer entrances, 17. sample inlets, 18. the 5th liang of three-way electromagnetic valves, 19. the 3rd three-way solenoid valves, 20. sampling needle cleaning devices, 21. sampling needles, 22. swab filtrators, 23. first hemolytic agent syringes, 24. sample injections devices, 25. second hemolytic agent syringes, 26. motors, 27. the 4th three-way solenoid valves, 28. the 5th three-way solenoid valves, 29. the 6th three-way solenoid valves, 30. three-way connections, 31. pinch valves, 32. reaction tanks, 33. insulated chambers, 34. the 6th liang of three-way electromagnetic valves, 35. the 7th liang of three-way electromagnetic valves, 36. gas pressure sensors, 37. the 8th liang of three-way electromagnetic valves, 38. pressure chambers, 39. the 9th liang of three-way electromagnetic valves, 40. three-way connections, 41. membrane pumps, 42. the 7th three-way solenoid valves, 43. three-way connections, 44. flow chambers.
Embodiment
Below in conjunction with Figure of description, the utility model is elaborated:
The utility model FCM analysis liquid-way system comprises dilution loading system, hemolysin and blood sample loading system and pressure system.
Dilution loading system comprises dilution bucket, dilution syringe, motor, liquid-pressure pick-up, flow chamber, reaction tank, comprises forebay and after-bay two parts, gem hole is housed between pond, front and back and does impedance counting use, overcoat HGB test lamp assembly and thermostat.Flow chamber is provided with the sample inlet pushed for sample stream, has the sheath streamer entrance for pushing away sheath stream, having the escape hole for sheath stream sample mixed liquor.Dilution syringe is connected with dilution bucket, makes dilution syringe can draw dilution from dilution bucket; Dilution syringe is connected with flow chamber sheath streamer entrance, makes dilution syringe can inject dilution and forms sheath stream to flow chamber; Dilution syringe is connected with reaction tank, makes dilution syringe can inject dilution to reaction tank; Dilution syringe is connected with sample injections device, and is also connected with cleaning device of sampling with sampling needle respectively through sample injections device, makes dilution syringe can inject dilution cleaning sampling needle inside and outside wall.Dilution syringe outlet is connected with liquid-pressure pick-up, and pressure that each the outside propelling movement liquid action of dilution syringe is produced can be monitored, in case hypertonia causes system to collapse pipe.
Hemolytic agent and blood sample loading system comprise: first and second hemolytic agent bottle, first and second hemolytic agent syringe, sample injections device, motor, sampling needle, sampling needle cleaning device.Two hemolytic agent syringes are connected with two hemolytic agent bottles respectively and other direction is all connected with reaction tank, make hemolytic agent syringe can draw two kinds of hemolytic agents respectively and join reaction tank respectively; Sample injections device is connected with flow chamber sample inlet, makes sample injections device sample can be injected flow chamber formation sample stream and is gone to detect by sheath stream parcel; Sample injections device is connected with sampling needle, makes sample injections device distribute blood sample by sampling needle collection; In hemolytic agent and blood sample loading system, two hemolytic agent syringes and sample injections device share a drive and control of electric machine, and two hemolytic agent syringes are symmetrically distributed in sample injections device both sides, and the resistance torque that three syringes are produced keeps balance;
Pressure system comprises: insulated chamber, pressure chamber, membrane pump, gas pressure sensor, swab filtrator.Pressure chamber is connected with flow chamber sample inlet, reaction tank, the sample in reaction tank can be drawn onto near sample inlet after making pressure chamber build negative pressure; Pressure chamber is connected with insulated chamber, and insulated chamber is connected with reaction tank, can emptying reaction tank when making pressure chamber emptying, when there is a malleation pressure chamber by insulated chamber to reaction tank bubble; The in-built filtration unit of insulated chamber, bulky grain in waste liquid after making reaction tank emptying is stopped by the in-built filtration unit of described insulated chamber with anti-clogging rear end pipe road system, and the rear end pollutant carried when simultaneously also can stop that pressure chamber is just pressing to reaction tank bubble enters reaction tank; Pressure chamber is connected with reaction tank after-bay, reaction tank after-bay and then be connected with dilution bucket, can draw dilution reach the effect of rinsing reaction tank after-bay when making pressure chamber build negative pressure through reaction tank after-bay from dilution bucket; Pressure chamber is connected with pressure transducer, makes can only reach desired value during pressure chamber's build-up pressure and non-superpressure causes system to collapse pipe; Membrane pump is connected with sampling needle cleaning device and in the pipeline be connected, is connected in series a swab filtrator, make membrane pump can waste liquid after pump drainage cleaning sampling needle and air-dry sampling needle outer wall simultaneously in waste liquid large granular impurity can not be blocked rear end pipe system by swab metre filter; Membrane pump two is all connected with pressure chamber, makes membrane pump can build negative pressure and malleation to pressure chamber;
FCM analysis liquid-way system also comprise first, second, third and fourth, five, six, seven three-way solenoid valves, first, second, third and fourth, five, six, seven, eight, nine liang of three-way electromagnetic valves and a pinch valve, pipe joint.First three-way solenoid valve common port is connected with liquid-pressure pick-up and then is connected with dilution syringe, and its Chang Kaiduan is connected with dilution bucket, and its normal-closed end is connected with the second three-way solenoid valve common port; Second three-way solenoid valve Chang Kaiduan is connected with second liang of three-way electromagnetic valve, and its normal-closed end is connected with flow chamber sheath streamer entrance.3rd three-way solenoid valve common port is connected with the 5th three-way solenoid valve Chang Kaiduan and then is communicated with sample injections device, and its Chang Kaiduan is connected with sampling needle cleaning device, and its normal-closed end is connected with sampling needle.4th three-way solenoid valve common port is connected with the first hemolytic agent syringe, and its Chang Kaiduan is connected with the first hemolytic agent bottle, and its normal-closed end is connected with reaction tank.5th three-way solenoid valve common port is connected with sample injections device, and its Chang Kaiduan is connected with the 3rd three-way solenoid valve common port, and its normal-closed end is connected with reaction tank with sample streamer entrance.6th three-way solenoid valve common port is connected with the second hemolytic agent syringe, and its Chang Kaiduan is connected with the second hemolytic agent bottle, and its normal-closed end is connected with reaction tank.7th three-way solenoid valve common port is connected with membrane pump endpiece, and its Chang Kaiduan communicates with waste liquid outlet, and its normal-closed end is connected with pressure chamber.First liang of three-way electromagnetic valve is arranged in the middle of dilution bucket and reaction tank after-bay.Second liang of three-way electromagnetic valve is arranged in the middle of the second three-way solenoid valve Chang Kaiduan and reaction tank.3rd liang of three-way electromagnetic valve is arranged between pressure chamber and sample streamer entrance.4th liang of three-way electromagnetic valve is arranged between flow chamber outlet and waste liquid outlet.5th liang of three-way electromagnetic valve is arranged between dilution syringe and sample injections device.6th liang of three-way electromagnetic valve is arranged between insulated chamber and pressure chamber.7th liang of three-way electromagnetic valve is arranged between reaction tank after-bay and pressure chamber.8th liang of three-way electromagnetic valve is arranged between sampling needle cleaning device and membrane pump.9th liang of three-way electromagnetic valve is arranged between pressure chamber and membrane pump.First three-way connection connects dilution bucket, first liang of three-way electromagnetic valve and the first three-way solenoid valve; Second three-way connection connecting fluid pressure sensor, the first three-way solenoid valve and the 5th liang of three-way electromagnetic valve; 3rd three-way connection connects the 5th three-way solenoid valve, sample inlet and reaction tank; 3rd three-way connection connects the 7th three-way solenoid valve, the 4th three-way solenoid valve and waste liquid outlet;
The utility model FCM analysis method adopts following steps:
The mat woven of fine bamboo strips one step sample collection: be communicated with sampling needle with sample injections device and draw blood sample, be injected into reaction tank; Be communicated with sampling needle by sample injections device and draw blood sample, then dilution is injected into sampling needle cleaning device by sample injections device by dilution syringe, at the Cleaning Process sampling needle outer wall that sampling needle rises, waste liquid is drained by the pipeline be connected on sampling needle cleaning device by membrane pump.
The mat woven of fine bamboo strips two step sample incubation reacts: add the first hemolytic agent with the first hemolytic agent syringe to reaction tank, then use two three-way electromagnetic valves of Bonding pressure room intermittent rapidly opened and closed to bubble in reaction tank, mixing blood sample and the first hemolytic agent, hatch after mixing, the concrete time comes fixed, and volume and velocity magnitude are all allow within the specific limits.The while of specimen needle being moved on in reaction tank, membrane pump builds malleation to pressure chamber, and hemolytic agent syringe adds the first hemolytic agent to reaction tank, then sample injections device point blood in reaction tank, the cleaning of sampling needle rising afterwards outer wall.Then two three-way electromagnetic valves that are connected with pressure chamber of reaction tank lower end are intermittent rapidly opened and closedly mixes blood sample and hemolytic agent to bubble in reaction tank.
The mat woven of fine bamboo strips three step sample prepares: after sample incubation, be drawn into the standby sample pipeline of flow chamber sample inlet by pressure chamber's negative pressure in reaction tank by sample through pinch valve; Hatch after mixing, when hatching, pressure chamber builds negative pressure.Opening two three-way electromagnetic valves be connected with sample inlet with pressure chamber after hatching end uses negative pressure from sample being sucked in reaction tank pinch valve to sample inlet, exceedes in the standby sample pipeline of inlet certain distance.
The mat woven of fine bamboo strips four step HGB measures: after standby sample is complete, the second hemolytic agent syringe adds the second hemolytic agent in reaction tank, pressure chamber builds malleation simultaneously, two three-way electromagnetic valves that are connected with pressure chamber of reaction tank lower end are intermittent afterwards rapidly opened and closedly mixes blood sample and the second hemolytic agent to bubble in reaction tank, then utilizes colourimetry to measure HGB value.
The mat woven of fine bamboo strips five step dilution blood sample, cleaning reaction pond, opens the emptying reaction tank of two three-way electromagnetic valves under membrane pump and insulated chamber, and then dilution syringe rinses to liquid feeding in reaction tank, so repeats once.To reaction tank under dilution syringe adds end liquid post-sampling pin to reaction tank afterwards, sample injections device divides blood to enter reaction tank, and pressure chamber builds malleation simultaneously.Two three-way electromagnetic valves that after point blood completes, reaction tank lower end is connected with pressure chamber are intermittent rapidly opened and closedly mixes blood sample and dilution to bubble in reaction tank, sampling needle rising dilution syringe beats liquid cleaning sampling needle outer wall simultaneously, needle point cleans sampling needle inwall after arriving in sampling needle cleaning device again, washes in pin inside and outside wall process and is taken away by waste liquid with membrane pump.Negative pressure is built to designated value to pressure chamber after washing pin.
The mat woven of fine bamboo strips six step cell detection: by dilution syringe and the drop-down appointment range of sample injections device, then dilution syringe pushes away sheath fluid to sheath streamer entrance, sample injections device pushes away sample stream to sample inlet, two syringes push away after certain hour is stablized by building the pressure in flow chamber simultaneously and open flow chamber outlet valve, then sheath stream parcel sample flows to and carries out the analysis of leucocyte five category measurement into flow chamber optical detecting parts, then does individual calculating according to the leukocytic quantity detected and the dilution ratio and volume that detect sample and just can converse leukocytic concentration in blood sample of patient.While formation sheath stream carries out testing, reaction tank is opened two three-way electromagnetic valve negative pressure that pressure chamber is connected with reaction tank after-bay over there and the sample mixed liquor of reaction tank forebay is sucked after-bay by gem hole is carried out impedance method counting RBC and PLT cell quantity, then with detection diluting blood sample than and volume converse RBC and PLT cell concentration in blood sample of patient.
The mat woven of fine bamboo strips seven step cleaning systems: cell detection all terminates rear dilution syringe and rinses to liquid feeding in flow chamber and associated ping and reaction tank thereof, membrane pump is by emptying forebay, pressure chamber waste liquid, finally build negative pressure by after-bay to dilution bucket pipeline imbibition cleaning after-bay, after cleaning, all parts return to measurement standby condition.FCM analysis method of the present utility model, a liquid-pressure pick-up is connected in series at the nearly endpiece of dilution syringe, all pressure doing to produce that surge that push away of dilution syringe are monitored and carried out pressure protect, the force value detected is reached the standard grade with the force value preset and is compared by control module, find to exceed standard and just release pressure in flow chamber by the control module valve stopping the action of all device parts then to open flow chamber outlet immediately of giving an order, the pipeline be connected with syringe can be made under the default conditions of other valves after quitting work to communicate with air and not have the phenomenon that builds the pressure.This pressure monitor mode effectively can judge that flow chamber detects micropore, sampling needle and each passage tube systems and whether blocks and clearly can point out blocking point, and can not only also be convenient to breakdown judge and maintenance by available protecting liquid-way system, principle as shown in Figure 1.Sample streamer porch is coupled together by two three-way electromagnetic valves and pressure chamber, make it possible to adopt the mode of negative pressure to carry out sample stream for sample, be different from other technical scheme dilution syringe for the mode of sample, decrease and test speed is improve to taking of dilution syringe.Simultaneously this mode be connected with pressure chamber can utilize negative pressure back suction flow chamber and specimen needle when flow chamber detects micropore or specimen needle blocks, and namely under suction function, the reverse direction with sheath stream and sample stream flows in pressure chamber and goes by liquid stream.
Embodiment, as shown in Figure 2, for making the purpose of this utility model, technical scheme and effect clearly, clearly, is described in further detail the utility model below.By the device Appropriate application of FCM analysis liquid-way system in the utility model, and by function reasonable distribution, devise a kind of simple and reliable, FCM analysis liquid-way system that test speed is fast.Should be appreciated that specific embodiment described herein only in order to explain the utility model, do not limit the utility model.
The utility model FCM analysis liquid-way system, as shown in Figure 2, comprises dilution loading system, hemolysin and blood sample loading system and pressure system.
Dilution loading system comprises dilution bucket 3, dilution syringe 7, motor 6, liquid-pressure pick-up 8, flow chamber 44, and the interior gem hole of reaction tank 32 does impedance counting, its outer suit HGB test lamp assembly and thermostat, three-way connection 4, three-way connection 9.Flow chamber 44 is provided with the sample inlet 17 pushed for sample stream, has the sheath streamer entrance 16 for pushing away sheath stream, having the escape hole 15 for sheath stream sample mixed liquor.Dilution syringe 7 is driven by motor 6, its outlet serial connection liquid-pressure pick-up 8, then three-way connection 9 is connected in series, connect the common port of the first three-way solenoid valve 10 again, be connected with three-way connection 4 by the Chang Kaiduan of the first three-way solenoid valve 10 and then be connected with dilution bucket 3, making dilution syringe 7 can draw dilution from dilution bucket 3; The normal-closed end of the first three-way solenoid valve 10 is connected with the second three-way solenoid valve 11 common port, and the normal-closed end of the second three-way solenoid valve 11 is communicated with flow chamber sheath streamer entrance 16, makes dilution syringe 7 can inject dilution and forms sheath stream to flow chamber 44; The escape hole 15 of flow chamber 44 is connected with the 4th liang of three-way electromagnetic valve 14, and the 4th liang of three-way electromagnetic valve 14 is communicated with waste liquid port by three-way connection 43, and the waste liquid that flow chamber 44 escape hole 15 is discharged can enter waste liquid barrel; Second three-way solenoid valve 11 Chang Kaiduan is connected with second liang of three-way electromagnetic valve 12, and second liang of three-way electromagnetic valve 12 is communicated with reaction tank 32 filling opening, makes dilution syringe 7 can inject dilution to reaction tank 32; Three-way connection 9 is connected to the 5th liang of three-way electromagnetic valve 18 and connects, 5th liang of three-way electromagnetic valve 18 is communicated with sample injections device 24, sample injections device 24 outlet is connected with the 5th three-way solenoid valve 28 common port, 5th three-way solenoid valve 28 Chang Kaiduan is connected with the 3rd three-way solenoid valve 19 common port, 3rd three-way solenoid valve 19 Chang Kaiduan is communicated with the inlet of sampling needle cleaning device 20,3rd three-way solenoid valve 19 normal-closed end is connected with sampling needle 21, makes dilution syringe 7 can inject dilution to clean sampling needle inside and outside wall like this.Dilution syringe 7 exports serial connection liquid-pressure pick-up 8, pressure that each outside propelling movement liquid action of dilution syringe 7 is produced can be monitored, especially sampling needle 21 is blocked up, sample injections device 24 blocks up, flow chamber 44 is stifled can report to the police by Timeliness coverage, and takes suddenly to stop pressure-relief measure in case hypertonia causes system to collapse pipe.
Hemolytic agent and blood sample loading system comprise the first hemolytic agent bottle 2, second hemolytic agent bottle 1, first hemolytic agent syringe 23, second hemolytic agent syringe 25, sample injections device 24, motor 26, sampling needle 21, sampling needle cleaning device 20, three-way connection 30.First hemolytic agent syringe 23, second hemolytic agent syringe 25, sample injections device 24 are by same motor 26 drived control, first hemolytic agent syringe 23 outlet is connected with the 4th three-way solenoid valve 27 common port, 4th three-way solenoid valve 27 Chang Kaiduan is communicated with the first hemolytic agent bottle 2,4th three-way solenoid valve 27 normal-closed end is communicated with reaction tank first hemolytic agent filling opening, makes the first hemolytic agent syringe 23 can draw the first hemolytic agent in the first hemolytic agent bottle 2 like this and joins reaction tank 32.Second hemolytic agent syringe 25 outlet is connected with the 6th three-way solenoid valve 29 common port, 6th three-way solenoid valve 29 Chang Kaiduan is communicated with the second hemolytic agent bottle 1,6th three-way solenoid valve 29 normal-closed end is communicated with reaction tank second hemolytic agent filling opening, makes the second hemolytic agent syringe 25 can draw the second hemolytic agent in the second hemolytic agent bottle 1 like this and joins reaction tank 32; Sample injections device 24 is connected with the 3rd three-way solenoid valve 19 common port by the 5th three-way solenoid valve 28 Chang Kaiduan, 3rd three-way solenoid valve 19 normal-closed end is communicated with sampling needle 21, sample injections device 24 outlet is connected with the 5th three-way solenoid valve 28 common port, 5th three-way solenoid valve 28 normal-closed end is connected with mouth on the right side of flow chamber sample inlet 17 by three-way connection 30, make sample injections device 24 sample can be injected flow chamber 44 formed sample stream by sheath stream parcel send to detection; Sample injections device 24 is made to gather by sampling needle 21, distribute blood sample; In hemolytic agent and blood sample loading system, two hemolytic agent syringes and sample injections device 24 share a drive and control of electric machine, two hemolytic agent syringes are symmetrically distributed in sample injections device 24 both sides, motor 26 is arranged on middle, and the resistance torque that three syringes are produced keeps balance;
Pressure system comprises insulated chamber 33, pressure chamber 38, membrane pump 41, gas pressure sensor 36, swab filtrator 22, three-way connection 43.Pressure chamber 38 is connected with the 3rd liang of three-way electromagnetic valve 13,3rd liang of three-way electromagnetic valve 13 is communicated with mouth on the left of flow chamber sample inlet 17, on the right side of flow chamber sample inlet 17, mouth is connected with reaction tank 32 by three-way connection 30, the pipeline that three-way connection 30 is connected with reaction tank 32 arranges a pinch valve 31, the sample in reaction tank 32 can be drawn onto after making pressure chamber 38 build negative pressure and exceed mouth one segment distance on the left of flow chamber sample inlet 17; Pressure chamber 38 is connected with the 6th liang of three-way electromagnetic valve 34,6th liang of three-way electromagnetic valve 34 is communicated with insulated chamber 33, insulated chamber 33 is communicated with reaction tank 32, can emptying reaction tank 32 when making pressure chamber 38 emptying, and pressure chamber 38 to build after malleation by insulated chamber 33 to reaction tank 32 bubble; The in-built filtration unit of insulated chamber 33, bulky grain in waste liquid after making reaction tank 32 emptying is stopped by the in-built filtration unit of described insulated chamber 33, with anti-clogging rear end pipe road system, also can stop that pressure chamber 38 enters reaction tank 32 to the rear end pollutant carried during bubble in reaction tank 32 after building malleation simultaneously; Pressure chamber 38 is connected with the 7th liang of three-way electromagnetic valve 35,7th liang of three-way electromagnetic valve 35 is connected with reaction tank 32 after-bay Single port, reaction tank 32 after-bay another port is connected with first liang of three-way electromagnetic valve 5 and then is connected with dilution bucket 3, dilution can be drawn through reaction tank 32 after-bay from dilution bucket 3 after making pressure chamber 38 build negative pressure and reach the effect of rinsing reaction tank 32 after-bay, built-in gem hole between reaction tank 32 forebay and after-bay, so can suck after-bay by the liquid of reaction tank 32 forebay by gem hole after pressure chamber 38 builds negative pressure complete impedance method counting; Pressure chamber 38 is connected with gas pressure sensor 36, make pressure chamber 38 build-up pressure in controlled range not superpressure cause system to collapse pipe; Membrane pump 41 suction side three-way connection 40 is communicated with, three-way connection 40 is connected with the 8th liang of three-way electromagnetic valve 37,8th liang of three-way electromagnetic valve 37 is connected with swab filtrator 22, swab filtrator 22 is communicated with sampling needle cleaning device 20 leakage fluid dram, make membrane pump 41 can waste liquid after pump drainage cleaning sampling needle and air-dry sampling needle outer wall simultaneously in waste liquid large granular impurity filtered by swab filtrator 22 and can not block rear end pipe system; Membrane pump 41 suction side is connected with the 9th liang of three-way electromagnetic valve 39 again by three-way connection 40, and the 9th liang of three-way electromagnetic valve 39 is communicated with pressure chamber 38, makes described membrane pump 41 can emptying pressure chamber 38 or set up negative pressure in pressure chamber 38; Described membrane pump 41 escape hole is connected with the 7th three-way solenoid valve 42 common port, and the 7th three-way solenoid valve 42 normal-closed end is communicated with pressure chamber 38, makes described membrane pump 41 can to the built-in pressure of attentioning in pressure chamber 38; 7th three-way solenoid valve 42 Chang Kaiduan is communicated with waste liquid outlet by three-way connection 43, and waste liquid can be discharged by described membrane pump 41.
The utility model FCM analysis method adopts following steps:
Sample collection: three-way solenoid valve 19 powers on and sample injections device 24 is communicated with sampling needle 21, sample injections device drop-down absorption aim parameter blood sample is in sampling needle, three-way solenoid valve 19 dead electricity after suction sample, inhale the sample drop-down suction dilution of dilution syringe 7 simultaneously, HGB background voltage is detected in reaction tank 32, after HGB background voltage tester terminates, membrane pump 41 and valve 39,34 power on emptying reaction tank; Then sampling needle rises in sampling needle cleaning device 20, simultaneously three-way solenoid valve 10 and two three-way electromagnetic valves 18 power on, then dilution is injected into sampling needle cleaning device 20 by sample injections device 24 by dilution syringe 7, at the Cleaning Process sampling needle outer wall that sampling needle rises, while cleaning, membrane pump 41, two three-way electromagnetic valve 37 powers on, and is drained by the waste liquid washing pin outer wall by membrane pump.
Sample incubation reacts: specimen needle to be moved on in reaction tank 32 process membrane pump 41, valve 37, valve 42 simultaneously and power on and build malleation to pressure chamber 38, gas pressure sensor 36 monitoring pressure value, after pressure room pressure reaches desired value, membrane pump 41 and valve 37,42 dead electricity stop by control module; Also have hemolytic agent syringe 23 to add the first hemolytic agent to reaction tank 32, then sample injections device 24 point blood in reaction tank, a point blood post-sampling pin 21 rises simultaneously, simultaneously with the same action cleaning needle outer wall that rises after sample collection.Then two three-way electromagnetic valves 34 that are connected with pressure chamber 38 of reaction tank 32 lower end are intermittent rapidly opened and closedly mixes blood sample and hemolytic agent to bubble in reaction tank 32, the quantitative dilution of the dilution drop-down suction of syringe 7 simultaneously, quantitative second hemolytic agent of the drop-down suction of the second hemolysin syringe 25.
Sample prepares: hatch a period of time after mixing, when hatching, membrane pump 41, valve 39 power on and build certain negative pressure to pressure chamber 38, gas pressure sensor 36 monitoring pressure value, after pressure room pressure reaches desired value, membrane pump 41 and valve 39 dead electricity stop by control module.Open two three-way electromagnetic valves 13 after hatching end to use negative pressure to be drawn onto by the sample after hatching in reaction tank 32 to exceed mouth on the left of sample inlet 17 and little by little, make sample be full of pinch valve to this section of sample inlet 17 for sample pipeline.
HGB measures: after sample finishes, the second hemolytic agent syringe 25 adds quantitative the second hemolytic agent in reaction tank 32, membrane pump 41 simultaneously, valve 37, valve 42 powers on and builds malleation to pressure chamber 38, gas pressure sensor 36 monitoring pressure value, after pressure room pressure reaches desired value, control module is by membrane pump 41 and valve 37, 42 dead electricity stop, after building pressure, two three-way electromagnetic valves 34 are intermittent rapidly opened and closedly mixes the residue sample and the second hemolytic agent made above to bubble in reaction tank, then colourimetry is utilized to measure HGB sample voltage value, HGB concentration is obtained through computing formula conversion by HGB background voltage above and current HGB sample voltage.
Cleaning reaction pond: HGB opens membrane pump 41, the emptying reaction tank of valve 34,39 after measuring, and then valve 10,12 powers on, and dilution syringe 7 rinses to liquid feeding in reaction tank, repeats once emptying, then adds liquid of the quantitative end to reaction tank 32.Simultaneously under sampling needle 21 to reaction tank 32, after adding end liquid, valve 19 powers on, sample injections device 24 points of quantitative blood enter reaction tank, reaction tank adds end liquid, and simultaneously membrane pump 41, valve 37, valve 42 power on and build malleation to pressure chamber 38, gas pressure sensor 36 monitoring pressure value, after pressure room pressure reaches desired value, membrane pump 41 and valve 37,42 dead electricity stop by control module.Point blood and build pressure complete after valve 34 intermittent rapidly opened and closed to bubble mixing blood sample and dilution in reaction tank 32, sampling needle 21 rising threshold 10,18 powers on simultaneously, dilution syringe 7 dozens of liquid cleaning sampling needle outer walls, after needle point arrives in sampling needle cleaning device, valve 19 powers on switching, allow dilution syringe 7 push away next dilution injection sampling needle inwall clean, the while of washing in pin inside and outside wall process, membrane pump 41, valve 37 power on, and are drained by the waste liquid washing pin inside and outside wall by membrane pump.After washing pin, immediately membrane pump 41, valve 39 power on and build certain negative pressure, gas pressure sensor 36 monitoring pressure value to pressure chamber 38, and after pressure room pressure reaches desired value, membrane pump 41 and valve 39 dead electricity stop by control module.
Cell detection: above-mentioned build negative pressure while by dilution syringe 7 and the drop-down appointment range of sample injections device 24, then valve 10, 11 power on, dilution syringe 7 pushes sheath fluid to sheath streamer entrance 16, sample injections device 24 pushes away sample stream to sample inlet 17 simultaneously, after two syringes push away certain hour simultaneously, flow chamber opens valve 14 after building the pressure and stablizing, then sheath stream parcel sample flows to and carries out the analysis of leucocyte five category measurement into flow chamber optical detecting parts, then do a mathematical computations according to the leukocytic quantity detected with the dilution ratio and volume that detect sample and just can converse leukocytic concentration in blood sample of patient.While formation sheath stream carries out leucocyte test, reaction tank 32 is opened valve 35 negative pressure over there and the sample mixed liquor of reaction tank forebay is carried out impedance method counting RBC and PLT cell quantity by gem hole suction after-bay, then converses RBC and PLT cell concentration in blood sample of patient with detection diluting blood sample ratio and volume.
Cleaning systems: both sides cell detection is all terminated rear dilution syringe 7 and rinsed to liquid feeding in reaction tank by valve 10,11,12; Dilution syringe 7 pushes away liquid cleaning flow chamber by valve 10,11,14 to flow chamber, dilution syringe 7 by valve 10,11, specimen needle in flow chamber, pinch valve 31 to reaction tank recoil specimen needle in flow chamber; Dilution syringe 7 cleans standby sample pipeline by valve 18, sample injections device 24, valve 28,13 to pressure chamber; Dilution syringe 7 passes through valve 18, sample injections device 24, valve 28, valve 19 to sampling needle 21 and sampling needle cleaning device 20 to clean sampling needle inside and outside wall; The waste liquid of cleaning sampling needle inside and outside wall is drained by valve 42 by valve 37, swab filtrator 22 to sampling needle cleaning device 20 by membrane pump 41; Waste liquid is discharged by valve 42 by valve 39, pressure chamber 38, valve 34, insulated chamber 33 to reaction tank by membrane pump 41; Then membrane pump 41 opens to the built-in negative pressure in pressure chamber 38 after-bay that dilution cleaning reaction pond 32 drawn by valve 35,5 from dilution bucket 3 by valve 39, and its waste liquid is discharged by pressure chamber 38, valve 39, membrane pump 41, valve 42; All syringes, valve pump after cleaning all parts and pipeline, sampling needle all returns to the wait of measurement standby condition and measures next time.

Claims (5)

1. a FCM analysis liquid-way system, comprises dilution loading system, hemolysin and blood sample loading system and pressure system, it is characterized in that:
Described dilution loading system is after driving dilution syringe (7) to be connected in series liquid-pressure pick-up (8) by motor (6), be connected dilution bucket (3) by the road with solenoid valve and draw dilution, be injected into flow chamber (44), reaction tank (32) respectively and be injected into sampling needle cleaning device (20) and sampling needle (21) through sample injections device (24);
Described hemolysin and blood sample loading system drive the first hemolytic agent syringe (23) by motor (26), second hemolytic agent syringe (25), sample injections device (24), described first hemolytic agent syringe (23) and the second hemolytic agent syringe (25) are connected the first hemolytic agent bottle (2) and the second hemolytic agent bottle (1) by the road respectively with solenoid valve, draw hemolytic agent and be injected into reaction tank (32), described sample injections device (24) is connected sampling needle (21) collection distribution blood sample by the road and is injected into reaction tank (32) with solenoid valve,
Described pressure system sets up positive/negative-pressure with solenoid valve Bonding pressure room (38) by the road by membrane pump (41), described membrane pump (41) is connected sampling needle cleaning device (20) pump drainage waste liquid with solenoid valve and swab filtrator (22) by the road, described pressure chamber (38) by the road with solenoid valve and pinch valve (31) coupled reaction pond (32), sample is drawn onto flow chamber (44) sample inlet, described pressure chamber (38) by the road with insulated chamber (33) coupled reaction pond (32), described pressure chamber (38) is connected dilution bucket (3) with after solenoid valve coupled reaction pond (32) after-bay by the road.
2. FCM analysis liquid-way system according to claim 1, is characterized in that gas pressure sensor (36) is equipped with in described pressure chamber (38).
3. FCM analysis liquid-way system according to claim 1, it is characterized in that point forebay and after-bay in described reaction tank (32), gem hole is housed between pond, front and back and does impedance counting, outside described reaction tank (32), be set with HGB test lamp assembly and thermostat.
4. FCM analysis liquid-way system according to claim 1, is characterized in that described first hemolytic agent syringe (23) and the second hemolytic agent syringe (25) are symmetrically distributed in sample injections device both sides.
5. FCM analysis liquid-way system according to claim 1, is characterized in that described insulated chamber (33) is built with filtration unit.
CN201520368054.3U 2015-05-30 2015-05-30 FCM analysis liquid-way system Withdrawn - After Issue CN204630897U (en)

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Cited By (7)

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CN104897557A (en) * 2015-05-30 2015-09-09 广州埃克森生物科技有限公司 Flow cytometry detection fluid circuit system and flow cytometry detection method
CN105717036A (en) * 2016-04-29 2016-06-29 安徽师范大学 Magnetic stirring type flow cytometry flowing chamber
CN105717034A (en) * 2016-02-01 2016-06-29 深圳开立生物医疗科技股份有限公司 Liquid path system and flow cytometry detection method thereof
CN107290556A (en) * 2016-03-31 2017-10-24 深圳迈瑞生物医疗电子股份有限公司 A kind of sample analyser
CN112798345A (en) * 2020-12-29 2021-05-14 深圳市科曼医疗设备有限公司 Pre-dilution mode sample collection and distribution system, method and blood cell analyzer
WO2022001370A1 (en) * 2020-06-30 2022-01-06 深圳市科曼医疗设备有限公司 Liquid path system for sheath flow, and control method
CN114660279A (en) * 2022-05-23 2022-06-24 深圳市帝迈生物技术有限公司 Sample detection method and sample analyzer

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104897557A (en) * 2015-05-30 2015-09-09 广州埃克森生物科技有限公司 Flow cytometry detection fluid circuit system and flow cytometry detection method
CN104897557B (en) * 2015-05-30 2018-03-09 广州埃克森生物科技有限公司 FCM analysis liquid-way system and FCM analysis method
CN105717034A (en) * 2016-02-01 2016-06-29 深圳开立生物医疗科技股份有限公司 Liquid path system and flow cytometry detection method thereof
CN107290556A (en) * 2016-03-31 2017-10-24 深圳迈瑞生物医疗电子股份有限公司 A kind of sample analyser
CN105717036A (en) * 2016-04-29 2016-06-29 安徽师范大学 Magnetic stirring type flow cytometry flowing chamber
CN105717036B (en) * 2016-04-29 2018-04-17 安徽师范大学 A kind of flow cytometer flow chamber of magnetic stirring
WO2022001370A1 (en) * 2020-06-30 2022-01-06 深圳市科曼医疗设备有限公司 Liquid path system for sheath flow, and control method
CN112798345A (en) * 2020-12-29 2021-05-14 深圳市科曼医疗设备有限公司 Pre-dilution mode sample collection and distribution system, method and blood cell analyzer
CN114660279A (en) * 2022-05-23 2022-06-24 深圳市帝迈生物技术有限公司 Sample detection method and sample analyzer

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