CN202119698U - Particle measurement device - Google Patents
Particle measurement device Download PDFInfo
- Publication number
- CN202119698U CN202119698U CN 201120236201 CN201120236201U CN202119698U CN 202119698 U CN202119698 U CN 202119698U CN 201120236201 CN201120236201 CN 201120236201 CN 201120236201 U CN201120236201 U CN 201120236201U CN 202119698 U CN202119698 U CN 202119698U
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- pipeline
- solenoid valve
- counting chamber
- chamber
- sample
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Abstract
The utility model relates to a particle measurement device, which belongs to the filed of analysis and detection of blood cells. A first counting chamber is mutually connected with a second counting chamber through a pipeline. A sample injector is connected with a sample injecting needle through a pipeline. The sample injecting needle is connected with a reaction tank pipeline through a magnetic valve. The second counting chamber is connected with a sample injector pipeline through a magnetic valve. A sheath liquid storage tank is connected with the sample injector pipeline through a magnetic valve and further connected with a first counting chamber pipeline through a magnetic valve. Furthermore, the sheath liquid storage tank is connected with a sheath flow chamber lateral connecting opening pipeline through a magnetic valve. The sheath flow chamber is connected with a waste liquid collecting bottle pipeline through a magnetic valve. The first counting chamber and the second counting chamber are provided with electrodes and gem micropores. The particle measurement device has the advantages of having novel structure, low failure rate of a system and small volume and reducing cost of equipment.
Description
Technical field
The utility model belongs to blood cell analyzing and testing field, refers in particular to the miniaturization five classification blood cell analyzers of a kind of fluidic cell method counting and electrical impedance method counting.
Background technology
Blood cell analyzer can detect the isoparametric quantity of leucocyte, red blood cell, blood platelet, haemoglobin in the blood and can classify to leucocyte.To finding and making a definite diagnosis some hematologic diseases and play certain reference role, be one of indispensable instrument of clinical examination.
Present stage, blood cell analyzer mainly was to adopt electrical impedance method or Flow Cytometry to count, classify, the mode that also has instrument to adopt two kinds of methods and deposit.
Electrical impedance method is with waiting the cell suspension that oozes the electrolyte solution dilution to insert in nonconducting container, the aperture pipe being injected in the cell suspension.Be full of electrolyte solution in the aperture pipe, and an interior electrode is arranged, an external electrode is arranged in the outside cell suspension of aperture pipe.After energized, be positioned at aperture pipe two lateral electrodes and produce steady current, the diluting cells suspension from the aperture pipe outside through jewel aperture on the aperture tube wall to the internal flow of aperture pipe; The interior resistance of aperture induction zone is increased, cause that transient voltage changes the formation pulse signal, pulse-response amplitude is high more; Cell volume is big more; Number of pulses is many more, and cell quantity is many more, draws haemocyte quantity and bulking value in the blood thus.
Flow Cytometry is a kind of technology of automatic analysis of cells, and its principle is the suspension that cell to be measured is prepared to individual cells, behind the specificity dyeing, puts into sample hose, gets into flow chamber by nozzle.Sheath fluid is full of flow chamber under certain pressure, cell is lined up single-row test section through flow chamber under the constraint of sheath fluid, when each cell passes through measurement zone, produce electric signal, and these signals can be represented fluorescence, light scattering, the impedance of light absorption or cell etc.These signals can be measured, storage, show, then a series of important physical characteristic of cell and biochemical characteristic just by apace, mensuration in large quantities.
Existing five differential count appearance mainly are to adopt two kinds of methods and the mode of depositing detects, and measure parameters such as RBC in the blood, PLT sum, volume through electrical impedance method, measure through Flow Cytometry to have a liking for acid among the WBC, have a liking for the classified information that alkali etc. is correlated with.Need a constant negative pressure source and proportioning device when electrical impedance method is measured, need a constant positive pressure source, this machine of will seeking common ground that the different system of two covers is arranged when Flow Cytometry is measured, system failure rate is high, and equipment cost is high, volume is big.
Summary of the invention
The utility model provides a kind of particle measurement device, and high to solve the existing equipment system failure rate, equipment cost is high, bulky problem.
The technical scheme that the utility model is taked is: counting chamber one, counting chamber two are connected mutually through pipeline with after-bay, and the after-bay water side in the counting chamber one is linked into the water intake end of after-bay in the counting chamber two, and the after-bay water inlet is connected with solenoid valve in the counting chamber one; Another termination sheath fluid liquid storage tank of solenoid valve, the water outlet termination solenoid valve of the after-bay in the counting chamber two, another termination sample syringe of solenoid valve; Sample syringe side interface is connected with the sheath fluid liquid storage tank through solenoid valve; The sample syringe is connected through pipeline with a notes appearance pin, annotates the appearance pin and is connected with the reaction tank pipeline through solenoid valve, and the sheath fluid liquid storage tank passes through solenoid valve and sheath stream chamber side interface pipeline is connected; Upper end, sheath stream chamber is connected with waste collection bottle pipeline through solenoid valve; There is the appearance of notes pin sheath stream lower end, chamber, and annotating appearance pin lower end is two-way joint, and an end is connected with the sample syringe; The other end is connected with reaction tank through solenoid valve, is separately installed with electrode, jewel micropore on counting chamber one, the counting chamber two.
The advantage of the utility model is: novel structure, system failure rate is low, has reduced equipment cost, volume is little.
Description of drawings
Fig. 1 is the structural representation of the utility model.
Embodiment
Counting chamber 1, counting chamber 22 and after-bay 10 are connected mutually through pipeline, and the after-bay water side in the counting chamber 1 is linked into the water intake end of after-bay in the counting chamber 22, and the after-bay water inlet is connected with solenoid valve 12 in the counting chamber 1; Solenoid valve 12 another termination sheath fluid liquid storage tanks 7, the water outlet termination solenoid valve 13 of the after-bay in the counting chamber 22, another termination sample syringe 4 of solenoid valve 13; Sample syringe 4 side interfaces are connected with sheath fluid liquid storage tank 7 through solenoid valve 15; Sample syringe 4 with annotate appearance pin 8 and be connected through pipeline, annotate appearance pin 8 and be connected with reaction tank 6 pipelines through solenoid valve 17, sheath fluid liquid storage tank 7 passes through solenoid valve 16 and sheath and flows chamber 5 side interface pipelines and be connected; Sheath stream 5 upper ends, chamber are connected with waste collection bottle 3 pipelines through solenoid valve 14; There is the appearance of notes pin 8 sheath stream 5 lower ends, chamber, and annotating appearance pin 8 lower ends is two-way joint, and an end is connected with sample syringe 4; The other end is connected with reaction tank 6 through solenoid valve 17, is separately installed with electrode 11, jewel micropore 9 on counting chamber 1, the counting chamber 22.
The utility model counting chamber one, two is used for electrical impedance method to be stored when counting and waits to count sample usefulness, and counting chamber contains the electrode that is used for the electrical impedance method counting simultaneously; When being used for flow cytometry, collects by waste collection bottle 3 waste liquid that flows out from sheath stream chamber 5; Sample syringe 4 is operated through push-and-pull and is accomplished flow cytometry and electrical impedance method counting; Sheath stream chamber 5 makes sheath fluid form sheath to flow and wrap up sample and carry out fluidic cell through detection zone and measure that sheath stream chamber 5 comprises the sheath fluid inlet port simultaneously, annotates appearance pin 8 and fluidic cell test section; The storage pool that sample to be tested reacted when reaction tank 6 was used for flow cytometry; Sheath fluid liquid storage tank 7 comprises the pressure source P that offers the sheath flowing pressure, is used to store the sheath fluid that forms sheath stream, also is used for the cleaning of each unit simultaneously; Electrode 11, jewel micropore 9 and after-bay 10 are installed on the counting chamber one, two, are used for the electrical impedance method counting.
The fluidic cell sample is prepared: solenoid valve 17 is opened, and the pipeline between reaction tank 6, notes appearance pin 8 and the sample syringe 4 is connected; Sample syringe 4 resorptions, the sample that reaction in the reaction tank 6 is good is drawn onto in the pipeline.In this process; Require sample to be full of pipeline, and require the end of sample to be no more than solenoid valve 17, the front end of sample can not enter in the sample syringe 4; It is used further to require sample in the pipeline to guarantee enough to be used for flow cytometry, and promptly the volume of pipeline is greater than the used volume of fluidic cell.After sample is ready, solenoid valve 17 cuts out.
The fluidic cell sample injects test: open solenoid valve 14, solenoid valve 16 and make sheath fluid at first get into flow chamber 5 to form sheaths stream.Promote sample syringe 4 with fast speeds earlier then; So that make as early as possible between flow chamber pressure and the sample syringe and reach pressure equilibrium; Can make in the pipeline ready sample be ejected into sheath stream chamber 5 again as early as possible through annotating an appearance pin 8; Reduce the speed of sample syringe 4 at last, make it reach a speed suitable, that can form better test condition, sample is tested through the test section by the sheath fluid parcel.After test finishes, stop sample syringe 4.
Stream type cell device cleans: after test finishes, need the dependence test position is cleaned.Open solenoid valve 15, make between sheath fluid liquid storage tank 7, sample syringe 4, pipeline, notes appearance pin 8, sheath stream chamber 5, the waste collection bottle 3 and form path, sheath fluid is driven in the waste collection bottle 3 along this road, clean this road, sample syringe 4 is raised to top simultaneously.Close solenoid valve 15, solenoid valve 16 after cleaning finishes, open solenoid valve 17, make sheath fluid liquid storage tank 7, sample syringe 4, pipeline, notes appearance pin 8, and reaction tank 6 between form path, come cleaning reaction pond 6 and associated pipe through the sheath fluid in the sheath fluid liquid storage tank 7.
The electrical impedance method test sample: solenoid valve 13 makes after-bay 10, pipeline, sample syringe 4 form path.Sample syringe 4 resorptions; With the sample to be tested in the counting chamber one, two be drawn onto after-bay 10 through jewel micropore 9 and the pipeline that is connected in, count with electrical impedance method, wherein the pipeline volume that is connected of two after-bays 10 should be greater than sample syringe 4 resorption volumes; Prevent that sample forms backflow in the counting chamber one in the after-bay of counting chamber two; Thereby influence sample counting in the counting chamber two, and volume also should prevent that sample from getting into solenoid valve 13 for a long time greater than sample syringe 4 resorption volumes in the pipeline.
The electrical impedance method sample cleans: need one after test is accomplished, counting chamber two, after-bay clean.Open solenoid valve 12, solenoid valve 15, solenoid valve 17; Make sheath fluid liquid storage tank 7 and counting chamber one, counting chamber two, reaction tank 6 form path; Thereby with the sheath fluid in the sheath fluid liquid storage tank 7 after-bay 10 in counting chamber one, the counting chamber two is cleaned, promote sample syringe 4 simultaneously to summit.After accomplishing cleaning, close all solenoid valves.
Claims (1)
1. particle measurement device, it is characterized in that: counting chamber one, counting chamber two are connected mutually through pipeline with after-bay, and the after-bay water side in the counting chamber one is linked into the water intake end of after-bay in the counting chamber two; The after-bay water inlet is connected with solenoid valve in the counting chamber one, another termination sheath fluid liquid storage tank of solenoid valve, the water outlet termination solenoid valve of the after-bay in the counting chamber two; Another termination sample syringe of solenoid valve, sample syringe side interface is connected with the sheath fluid liquid storage tank through solenoid valve, and the sample syringe is connected through pipeline with a notes appearance pin; Annotating the appearance pin is connected with the reaction tank pipeline through solenoid valve; The sheath fluid liquid storage tank is connected with sheath stream chamber side interface pipeline through solenoid valve, and upper end, sheath stream chamber is connected with waste collection bottle pipeline through solenoid valve, and there is the appearance of notes pin sheath stream lower end, chamber; Annotating appearance pin lower end is two-way joint; One end is connected with the sample syringe, and the other end is connected with reaction tank through solenoid valve, is separately installed with electrode, jewel micropore on counting chamber one, the counting chamber two.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120236201 CN202119698U (en) | 2011-07-06 | 2011-07-06 | Particle measurement device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120236201 CN202119698U (en) | 2011-07-06 | 2011-07-06 | Particle measurement device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202119698U true CN202119698U (en) | 2012-01-18 |
Family
ID=45460753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120236201 Expired - Lifetime CN202119698U (en) | 2011-07-06 | 2011-07-06 | Particle measurement device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202119698U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699299A (en) * | 2016-01-28 | 2016-06-22 | 陈晓乾 | Method and device for measuring content of red blood cells and content of hemachrome |
| CN105699434A (en) * | 2016-01-28 | 2016-06-22 | 陈晓乾 | Method and device for measuring content of blood platelets |
| CN106814183A (en) * | 2016-12-27 | 2017-06-09 | 深圳开立生物医疗科技股份有限公司 | A kind of sheath stream detecting system and sheath method of flow control |
| CN107843542A (en) * | 2017-11-27 | 2018-03-27 | 桂林优利特医疗电子有限公司 | The specimen cup that a kind of cellanalyzer uses |
| CN109085019A (en) * | 2018-08-03 | 2018-12-25 | 迪瑞医疗科技股份有限公司 | A kind of sample processing apparatus and method |
-
2011
- 2011-07-06 CN CN 201120236201 patent/CN202119698U/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699299A (en) * | 2016-01-28 | 2016-06-22 | 陈晓乾 | Method and device for measuring content of red blood cells and content of hemachrome |
| CN105699434A (en) * | 2016-01-28 | 2016-06-22 | 陈晓乾 | Method and device for measuring content of blood platelets |
| CN105699299B (en) * | 2016-01-28 | 2018-02-27 | 陈晓乾 | A kind of red blood cell content and hemoglobin assay method and measure device |
| CN105699434B (en) * | 2016-01-28 | 2018-03-23 | 陈晓乾 | A kind of assay method of platelet content and measure device |
| CN106814183A (en) * | 2016-12-27 | 2017-06-09 | 深圳开立生物医疗科技股份有限公司 | A kind of sheath stream detecting system and sheath method of flow control |
| CN106814183B (en) * | 2016-12-27 | 2019-07-16 | 深圳开立生物医疗科技股份有限公司 | A kind of sheath stream detection system and sheath method of flow control |
| CN107843542A (en) * | 2017-11-27 | 2018-03-27 | 桂林优利特医疗电子有限公司 | The specimen cup that a kind of cellanalyzer uses |
| CN109085019A (en) * | 2018-08-03 | 2018-12-25 | 迪瑞医疗科技股份有限公司 | A kind of sample processing apparatus and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 130012 Changchun province high tech Zone, the River Street, No. 95, No. Patentee after: Medical Polytron Technologies Inc Address before: 130012 Changchun province high tech Zone, the River Street, No. 95, No. Patentee before: Changchun Dirui Medical Technology Co., Ltd. |
|
| CP01 | Change in the name or title of a patent holder | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120118 |
|
| CX01 | Expiry of patent term |