CN110118717A - The liquid channel system and its application method and flow cytometer of flow cytometer - Google Patents
The liquid channel system and its application method and flow cytometer of flow cytometer Download PDFInfo
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- CN110118717A CN110118717A CN201910449925.7A CN201910449925A CN110118717A CN 110118717 A CN110118717 A CN 110118717A CN 201910449925 A CN201910449925 A CN 201910449925A CN 110118717 A CN110118717 A CN 110118717A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
Abstract
The invention discloses a kind of liquid channel system of flow cytometer and its application method and flow cytometers.The liquid channel system includes test device, sampling device, sheath liquid device, negative pressure device and cleaning device, and test device includes the flow chamber for connecting detection analysis system and the emptying control valve that is located at the liquid outlet of flow chamber；Sampling device include the loading pipeline for being connected to flow chamber, the specimen needle that is located on loading pipeline with sample control valve, be connected to the detection pipeline of loading mouth and is located at the quantitative part detected on pipeline；Sheath liquid device includes the sheath fluid pipeline for being connected to the inlet of flow chamber, the first sheath hydraulic control valve being located on detection pipeline and the sheath liquid pump, sheath fluid pressure sensor, the second sheath hydraulic control valve that are located on sheath fluid pipeline, and detection pipeline is also connected to sheath fluid pipeline by the position between sheath liquid pump and the second sheath hydraulic control valve；The negative pressure mouth of negative pressure device connection flow chamber；Cleaning device is for cleaning specimen needle.The liquid channel system is easy to operate and at low cost.
Description
Technical field
The present invention relates to field of biological detection, more particularly to the liquid channel system and its application method of a kind of flow cytometer
With flow cytometer.
Background technique
Flow cytometer (Flow Cytometer) is glimmering by the scattering light and label of measurement cell and other biologic grains
Luminous intensity quickly analyzes particle physically or chemically, can measure multiple characteristic parameters from a cell simultaneously, carry out
Qualitative or quantitative analysis has the characteristics that speed is fast, precision is high, accuracy is good, and flow cytometer is mainly by liquid fluid system, optics
System, electronic system, data analysis system composition.Basic system of the liquid channel system as flow cytometer, will be containing by fluorescence
The cell of dyeing or the sample liquid of particle are transported to flow chamber together with sheath fluid, so that cell or particle is existed using liquid stream focusing principle
Sample flow is formed under laminar condition, cell or particle pass sequentially through flow chamber detection zone.Stable sample flow is follow-up signal
One of guarantee of detection, and entire instrument core technology.
The flow cytometer of mainstream on Vehicles Collected from Market has the function of absolute counting mostly, and there are mainly two types of counting modes,
One kind is that absolute volume counts, and another kind is that relative volume counts.Absolute volume counting, which refers to, flows through flow chamber by metering
Sample volume, which is realized, to be counted, and comparative counting refers to calculates sample according to the quantity of the particle of the known concentration by flow chamber indirectly
This volume.Comparative counting mode is due to high costs, and many emerging instrument manufacturer facility men take absolutely on Vehicles Collected from Market
The mode of volumetric enumeration.There are many kind, the functional multiple functional person often sides of realization again for the implementation that absolute volume counts
Formula complexity, higher cost；When cost is relatively low person or performance cannot be guaranteed (consistency is poor, carry pollution high) or cannot be long
Between continue loading or it is complicated for operation, the degree of automation is low.
Summary of the invention
Based on this, it is necessary to provide that a kind of high degree of automation, structure is simple, streaming easy to operate and at low cost is thin
The liquid channel system and its application method and flow cytometer of born of the same parents' instrument.
A kind of liquid channel system of flow cytometer, comprising:
Test device, the test device include the flow chamber for connecting detection analysis system and are located at the flowing
Emptying control valve at the liquid outlet of room；
Sampling device, the sampling device include the loading pipeline for being connected to the loading mouth of the flow chamber, are located on described
Specimen needle on sample pipeline with sample control valve, be connected to the detection pipeline of the loading mouth and be located on the detection pipeline
Quantitative part；
Sheath liquid device, the sheath liquid device include the sheath fluid pipeline for being connected to the inlet of the flow chamber, are located at the inspection
The first sheath hydraulic control valve on test tube road and sheath liquid pump, sheath fluid pressure sensor, the second sheath being located on the sheath fluid pipeline
Hydraulic control valve, the detection pipeline are also connected to the sheath fluid by the position between the sheath liquid pump and the second sheath hydraulic control valve
Pipeline；And
Negative pressure device, the negative pressure device are connected to the negative pressure mouth of the flow chamber.
The sampling device further includes sampling needle in one of the embodiments, and one end of the loading pipeline connects institute
Sampling needle is stated, the other end of the loading pipeline is connected to the loading mouth by the specimen needle.
The liquid channel system of the flow cytometer further includes cleaning device in one of the embodiments, the cleaning dress
Set including be located in the specimen needle cleaning part, one end is by the position between the sheath liquid pump and the second sheath hydraulic control valve
It is connected to the sheath fluid pipeline and the other end connects the cleaning pipeline of the cleaning part and is located at the cleaning cleaned on pipeline
Control valve.
The sheath liquid device further includes sheath liquid filter in one of the embodiments,；The sheath liquid filter is located at institute
It states on sheath fluid pipeline and between the sheath liquid pump and the second sheath hydraulic control valve, the cleaning pipeline is connected to the sheath fluid
The exhaust channel of filter, irrigation source of the gas being discharged in the exhaust channel of the sheath liquid filter as the cleaning part.
The negative pressure device includes being connected to the waste solution channel of the negative pressure mouth and being located at institute in one of the embodiments,
State waste drains pump, waste liquid pressure sensor and the waste liquid control valve on waste solution channel, the waste liquid control valve is close to the stream
Dynamic room, the waste solution channel are also connected to the liquid outlet, and the waste liquid control valve is in parallel with the emptying control valve.
The negative pressure device further includes waste fluid container in one of the embodiments,；The waste liquid pool is connected to the waste liquid
Pipeline, the waste liquid pressure sensor are located on the waste liquid pool.
The negative pressure device further includes waste liquid pool in one of the embodiments,；The waste liquid pool is connected to the sewer pipe
Road, the waste liquid pressure sensor are located on the waste liquid pool, and the waste liquid pool is connected by the waste solution channel, the waste drains pump
It is connected to the waste fluid container.
A kind of application method of the liquid channel system of the flow cytometer, includes the following steps:
Close emptying control valve, sample control valve, the first sheath hydraulic control valve, the second sheath hydraulic control valve, purge control valve；
When the liquid channel system of flow cytometer is in continuous measurement pattern, negative pressure device is opened, opens sample control valve,
Sample liquid passes through sample unit under the action of negative pressure, sample control valve enters flow chamber；The second sheath hydraulic control valve is opened, sheath is passed through
Liquid pump is pumped into sheath fluid and the pressure value of sheath fluid pressure sensor is made to reach setting pressure Pa；Sample liquid is formed under the package of sheath fluid
Stable sample flow is tested by the measurement part of the flow chamber, the waste liquid discharge after measurement；
When the liquid channel system of flow cytometer is in the test of absolute counting mode, sample control valve, sample unit are opened
Into the sample liquid in sample tube liquid level hereinafter, by quantitative part will predetermined volume sample liquid sucking detection pipeline in；
Sample control valve is closed, negative pressure device is opened, quantitative part works so that the sample liquid in detection pipeline is opened full of loading pipeline
The first sheath hydraulic control valve is opened, sheath fluid is pumped by sheath liquid pump, the pressure value of sheath fluid pressure sensor is made to reach setting pressure Pa,
Sample liquid forms stable sample flow under the package of sheath fluid and is tested by the measurement part of the flow chamber, after measurement
Waste liquid discharge；If after the sample liquid in the quantitative part is tested, the stopping test condition not up to setting then opens sample
Control valve, sample liquid continue to test by the measurement part that the sample unit, the sample control valve enter the flow chamber；
When cleaning sampling device, sheath fluid is pumped by sheath liquid pump and enters cleaning part by cleaning pipeline, sheath fluid enters clear
The outer wall of part cleaning sample unit is washed, cleaning waste liquid is discharged by the outlet end of cleaning part.
A kind of flow cytometer, including optical system, electronic system, testing and analysis system and the liquid fluid system；
The optical system is connected to the flow chamber of the liquid fluid system for irradiating the indoor sample liquid of flowing
So that the cell of specific fluorescence dyeing issues fluorescence after being stimulated；The electronic system is for being converted to the fluorescence signal
Electric signal simultaneously amplifies, and the testing and analysis system connects the electronic system for obtaining the amplified electric signal with complete
The analysis of the cell of pairs of specific fluorescence dyeing.
The liquid channel system of above-mentioned flow cytometer have bubble fully functional, in automatic-discharging system, structure it is simple, at
This low feature.Compared to the liquid channel system of traditional flow cytometer, the liquid channel system of flow cytometer of the invention such as just like
It is lower the utility model has the advantages that
1, fully functional.The present invention has the function of that Vehicles Collected from Market mainstream flow cytometer is had, including absolute counting,
And the continuous measurement for a long time of the ground of seamless connection, different measuring speeds and speed manually adjust.
2, the bubble degree in automatic-discharging system is high.The present invention compared with mainstream flow cytometer on Vehicles Collected from Market,
With the bubble that may add up in real-time emptying liquid channel system, so that further the test function of systems stabilisation initializes, is standby
Maintenance, reagent real-time display and early warning, fluid path charge, empty, fluid path exhaust bubble, fault alarm with from functions such as eliminations.
3, superior performance.The unique measurement fluid path of the present invention, so that sample flow is more stable；Unique filtration system, so that
Fluid path is purer, and noise is lower, and resolution ratio is more preferably；Unique cleaning way makes in the case where not increasing system maintenance time
Residual contamination rate it is lower, between sample measurement be independent of each other, utmostly eliminate in liquid channel system bubble accumulation false positive,
The error results such as false negative.
4, structure is simple, at low cost.Mainstream flow cytometer all has system complex on Vehicles Collected from Market, and components price is high
High, maintenance and the high feature of maintenance cost, the present invention select less components to the greatest extent, and components have very
Big versatility, maintenance, repair are at low cost；Meanwhile the present invention passes through the combination between optimization components, reasonable utilization space
The modes such as arrangement reduce components type and quantity to the greatest extent, so that system structure is simple, thus low in cost, reliability is more
It is high.
Detailed description of the invention
Fig. 1 is the schematic diagram of the liquid channel system of flow cytometer described in an embodiment.
Description of symbols
10: the liquid channel system of flow cytometer；100: test device；110: flow chamber；111: loading mouth；112: going out liquid
Mouthful；113: negative pressure mouth；114: inlet；120: emptying control valve；200: sampling device；210: loading pipeline；220: specimen needle；
230: sample control valve；240: detection pipeline；250: quantitative part；260: sampling needle；300: sheath liquid device；310: sheath liquid pipe
Road；320: the first sheath hydraulic control valves；330: the second sheath hydraulic control valves；340: sheath liquid pump；350: sheath fluid pressure sensor；360: sheath
Liquid container；370: sheath liquid filter；400: negative pressure device；410: waste solution channel；420: waste drains pump；430: waste liquid pressure sensing
Device；440: waste liquid control valve；450: waste fluid container；460: waste liquid pool；500: cleaning device；510: cleaning part；520: cleaning pipe
Road；530: purge control valve；540: drain pipe；550: positive displacement pump.
Specific embodiment
To facilitate the understanding of the present invention, a more comprehensive description of the invention is given in the following sections with reference to the relevant attached drawings.In attached drawing
Give presently preferred embodiments of the present invention.But the invention can be realized in many different forms, however it is not limited to this paper institute
The embodiment of description.On the contrary, purpose of providing these embodiments is keeps the understanding to the disclosure more thorough
Comprehensively.
It should be noted that it can directly on the other element when element is referred to as " being fixed on " another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it, which can be, is directly connected to
To another element or it may be simultaneously present centering elements.When an element is considered as " being located at " another element, it can
To be directly to another element or may be simultaneously present centering elements.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
Any and all combinations of the listed item of pass.
One embodiment of the invention provides a kind of flow cytometer comprising optical system, tests and analyzes system at electronic system
System and liquid fluid system.
Shown in Figure 1, the liquid channel system 10 of flow cytometer includes test device 100, sampling device 200, sheath fluid dress
Set 300, negative pressure device 400 and cleaning device 500.
Test device 100 includes the flow chamber 110 for connecting detection analysis system and the liquid out for being located at flow chamber 110
Emptying control valve 120 at mouth 112.
Sampling device 200 includes the loading pipeline 210 of the loading mouth 111 of connection flow chamber 110, is located at loading pipeline 210
On specimen needle 220 with sample control valve 230, be connected to the detection pipeline 240 of loading mouth 111 and be located at detection pipeline 240 on
Quantitative part 250.
Sheath liquid device 300 includes the sheath fluid pipeline 310 of the inlet 114 of connection flow chamber 110, is located at detection pipeline 240
On the first sheath hydraulic control valve 320 and the sheath liquid pump 340, the sheath fluid pressure sensor 350, second that are located on sheath fluid pipeline 310
Sheath hydraulic control valve 330, detection pipeline 240 are also connected to sheath liquid pipe by the position between sheath liquid pump 340 and the second sheath hydraulic control valve 330
Road 310.
The negative pressure mouth 113 of the connection flow chamber 110 of negative pressure device 400.Negative pressure device 400 includes the useless of connection negative pressure mouth 113
Liquid pipe road 410 and the waste drains pump 420 being located on waste solution channel 410, waste liquid pressure sensor 430 and waste liquid control valve 440,
For waste liquid control valve 440 close to flow chamber 110, waste solution channel 410 is also connected to liquid outlet 112, and waste liquid control valve 440 and emptying are controlled
Valve 120 processed is in parallel.
Further, sampling device 200 further includes sampling needle 260, and one end of loading pipeline 210 connects sampling needle 260, on
The other end of sample pipeline 210 is connected to loading mouth 111 by specimen needle 220.
Cleaning device 500 includes the cleaning part 510 being located in specimen needle 220, one end by sheath liquid pump 340 and the second sheath hydraulic control
Position connection sheath fluid pipeline 310 and the other end between valve 330 processed connect the cleaning pipeline 520 of cleaning part 510 and are located at clear
Wash the purge control valve 530 on pipeline 520.Cleaning part 510 can be swab.
Sheath liquid device 300 further includes sheath liquid filter 370；Sheath liquid filter 370 is located on sheath fluid pipeline 310 and is located at sheath
Between liquid pump 340 and the second sheath hydraulic control valve 330, cleaning pipeline 520 is connected to the exhaust channel of sheath liquid filter 370.The present invention
Using the exhaust channel of sheath liquid filter 370 as the irrigation source of cleaning part 510, gas in sheath liquid filter 370 not only can guarantee
Body is discharged in time, also under the premise of not influencing cleaning function, simplifies the quantity of liquid channel system pipeline and valve.
Wherein, inlet 114, liquid outlet 112 are located at the side of flow chamber 110, and negative pressure mouth 113 is located at flow chamber
110 top, the bottom of 111 flow chamber 110 of loading mouth.
In a specific example, cleaning device 500 further includes drain pipe 540 and the positive displacement pump that is located on drain pipe 540
550, drain pipe 540 connects cleaning part 510.
In a specific example, negative pressure device 400 further includes waste fluid container 450.Waste solution channel 410 connects flow chamber 110
End be connected to waste fluid container 450.
Further, negative pressure device 400 further includes waste liquid pool 460.Waste liquid pool 460 is connected to waste solution channel 410, waste liquid pressure
Sensor 430 is located on waste liquid pool 460.Waste liquid pool 460 is connected to waste fluid container 450 by waste solution channel 410, waste drains pump 420.
Further, test device 100 further includes liquid outlet pipe.One end connection liquid outlet 112 and another of liquid outlet pipe
End connection waste solution channel 410, emptying control valve 120 are located in liquid outlet pipe.
In a specific example, sheath liquid device 300 further includes sheath liquid container 360.Sheath fluid pipeline 310 is connected to sheath liquid container
360。
Optical system is for irradiating the sample liquid being connected within the flow chamber 110 of liquid fluid system so that specific fluorescence contaminates
The cell or particle of color issue fluorescence after being stimulated, and receive issued fluorescence signal.
Electronic system is for being converted to electric signal for fluorescence signal and amplifying.
Testing and analysis system connects electronic system to complete to dye specific fluorescence for obtaining amplified electric signal
Cell or particle analysis.
Waste liquid pool 460 in the liquid channel system 10 of flow cytometer of the invention via inlet 114 and liquid outlet 112 with
And waste liquid pressure sensor 430, and respond rapidly, avoid the too long waiting time.Negative pressure device 400 can be by slave computer (PLC
Driver Card) directly controlled, constant pressure, fast response time are maintained after booting, regardless of whether other fluid path are accessed,
It all can guarantee that pressure is stablized, control is simple and reliable, avoids being influenced to lead to control disorder by other factors.
The present invention has the function of that absolute counting and continuous measurement function, absolute counting will be by quantitative in terms of testing process
Component (for example, syringe pump) realizes volume quantitative；Continuous measurement is then directly real by the arrival of loading pipeline 210 flow chamber 110
It is existing.The difference is that the present invention still can be measured continuously after the test for completing absolute counting.It is surveyed if necessary to more
Data are measured, after the test sample in quantitative part, only need to open sample control valve 230, into continuous measurement pattern, i.e.,
Phenomena such as continuous measurement pattern can be steadily switched to, not will cause sample flow mutation or cutout.
A kind of application method of the liquid channel system 10 of flow cytometer, includes the following steps:
The liquid channel system 10 of flow cytometer by no matter test whether, negative pressure device 400 maintains fixed negative pressure Pm.
Maintain the pressure in negative pressure device in range of set value；Specifically, it is supervised by 430 moment of waste liquid pressure sensor
The pressure of waste liquid pool 460 is controlled, and maintains the pressure in waste liquid pool 460 to maintain range of set value always together with waste drains pump 420
It is interior.
It is shown in Figure 1, when the sheath fluid filter exhaust of flow cytometer bubble, open purge control valve 530, positive displacement pump
550, it is filled by sheath liquid pump 340 to sheath liquid filter 370, the bubble or liquid in sheath liquid filter 370 pass through clear
It washes control valve 530 to flow in cleaning part 510, sheath fluid is discharged by positive displacement pump 550 in cleaning part 510, charges to sheath liquid filter 370 full
Until bubble-free.
When 260 outer wall of sampling needle of flow cytometer needs to clean, purge control valve 530 and positive displacement pump 550 are opened,
Sheath fluid flow to sheath liquid filter by sheath liquid pump 340, and then the exhaust outlet outflow through filter 370, passes through purge control valve 530
Reach cleaning part 510 in, sheath fluid in cleaning part 510 after special flushing channel is to 260 outer wall washing of sampling needle, waste liquid
It is drained into waste fluid container 450 under the action of positive displacement pump 550 via drain pipe 540 by the apocenosis passage of cleaning part 510.Pass through
The cleaning to sampling needle outer wall can be completed in the relative motion of sampling needle 260 and cleaning part 510, while if in sheath liquid filter
There are micro bubbles to be also discharged together, forms a large amount of bubbles after avoiding micro bubble from accumulating for a long time, influences the steady of instrument test
Qualitative and accuracy.
When the liquid channel system 10 of flow cytometer is in continuous measurement pattern, (the i.e. unlatching waste liquid of negative pressure device 400 is opened
Control valve 440), negative pressure can be kept in flow chamber 110, opens sample control valve 230, sample liquid passes through sample under the action of negative pressure
This needle 220, sample control valve 230 and sampling needle 260 rapidly enter flow chamber 110；The second sheath hydraulic control valve 330 is opened, is passed through
Sheath liquid pump 340 is pumped into sheath fluid and the pressure value of sheath fluid pressure sensor 350 is made to reach setting pressure Pa；After sheath hydraulic coupling is stablized,
Sample liquid forms stable sample flow under the package of sheath fluid and is tested by the measurement part of flow chamber 110 at this time, measures
Enter in waste liquid pool 460 after waste liquid discharge afterwards, is discharged in such as waste liquid barrel of waste fluid container 450 by waste drains pump 420；It needs to illustrate
Be sheath hydraulic coupling (Pa) set in negative pressure set by negative pressure device 400 (Pm) and sheath liquid device 300, by specific liquid
The length and internal diameter of road system connecting line codetermine.
When the liquid channel system 10 of flow cytometer is in the test of absolute counting mode, sample control valve 230, sampling are opened
Needle 260 enters the liquid level of the sample liquid in sample tube hereinafter, the sample liquid of predetermined volume is sucked detection by quantitative part 250
In pipeline 240；Sample control valve 230 is closed, waste liquid control valve 440 is opened, to examine under the impetus of quantitative part 250
Sample liquid in test tube road 240 opens the first sheath full of the loading pipeline between sample control valve 230 and flow chamber 110
Hydraulic control valve 320 is pumped into sheath fluid by sheath liquid pump 340 and the pressure value of sheath fluid pressure sensor 350 is made to reach setting pressure Pa,
After sheath hydraulic coupling is stablized, under the continuing to press on of quantitative part, the stable sample flow that is wrapped to form through sheath fluid passes through sample liquid
The measurement part of flow chamber 110 is tested, and is entered in waste liquid pool 460 after the waste liquid discharge after measurement, by 420 row of waste drains pump
Into waste fluid container 450 such as waste liquid barrel)；After if the sample liquid in quantitative part 250 is tested, the measurement data that not up to sets
(stopping test condition), then open sample control valve 230, and sample liquid passes through specimen needle 220, sample control valve 230 and sampling needle
The 260 measurement parts for rapidly entering flow chamber 110 continue to test, and due in handoff procedure, waste liquid pressure remained constant is constant, sheath
Hydraulic coupling remains invariable therefore sample loading speed remains unchanged, and entirely switches stable and continuous, no to stop, without mutation.
The liquid channel system 10 of above-mentioned flow cytometer has fully functional, high degree of automation, structure simple, at low cost
Feature.Compared to the liquid channel system 10 of traditional flow cytometer, the liquid channel system 10 of flow cytometer of the invention is if any as follows
The utility model has the advantages that
1, fully functional.The present invention has the function of that Vehicles Collected from Market mainstream flow cytometer is had, including absolute counting,
And seamless connection ground continuous measurement for a long time.
2, the bubble in automatic-discharging system.The present invention has real-time compared with mainstream flow cytometer on Vehicles Collected from Market
The bubble that may add up in emptying liquid channel system, thus the further test function of systems stabilisation.
3, superior performance.The unique measurement fluid path of the present invention, so that sample flow is more stable；Unique filtration system, so that
Fluid path is purer；Unique cleaning way utmostly eliminates liquid channel system in the case where not increasing system maintenance time
In bubble accumulation.
4, structure is simple.Mainstream flow cytometer all has system complex on Vehicles Collected from Market, and components are at high price, maintenance
And the feature that maintenance cost is high, the present invention select less components to the greatest extent, maintenance, repair are at low cost；Together
When, the present invention reduces components type to the greatest extent by optimizing the modes such as combination, reasonable utilization space layout between components
And quantity, so that system structure is simple, and low in cost, high reliablity.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (9)
1. a kind of liquid channel system of flow cytometer characterized by comprising
Test device, the test device include the flow chamber for connecting detection analysis system and are located at the flow chamber
Emptying control valve at liquid outlet；
Sampling device, the sampling device include the loading pipeline for being connected to the loading mouth of the flow chamber, are located at the loading pipe
Specimen needle on road with sample control valve, be connected to the detection pipeline of the loading mouth and be located at quantifying on the detection pipeline
Component；
Sheath liquid device, the sheath liquid device include the sheath fluid pipeline for being connected to the inlet of the flow chamber, are located at the detection pipe
The first sheath hydraulic control valve on road and sheath liquid pump, the sheath fluid pressure sensor, the second sheath hydraulic control being located on the sheath fluid pipeline
Valve processed, the detection pipeline are also connected to the sheath liquid pipe by the position between the sheath liquid pump and the second sheath hydraulic control valve
Road；And
Negative pressure device, the negative pressure device are connected to the negative pressure mouth of the flow chamber, by waste liquid control valve to being in negative pressure state
Waste liquid pool.
2. the liquid channel system of flow cytometer according to claim 1, which is characterized in that the sampling device further includes adopting
One end of sample needle, the loading pipeline connects the sampling needle, and the other end of the loading pipeline is connected to by the specimen needle
The loading mouth.
3. the liquid channel system of flow cytometer according to claim 2, which is characterized in that the fluid path of the flow cytometer
System further includes cleaning device, and the cleaning device includes the cleaning part being located in the specimen needle, one end by the sheath liquid pump
Position between the second sheath hydraulic control valve is connected to the sheath fluid pipeline and the other end connect the cleaning pipe of the cleaning part
Road and the purge control valve being located on the cleaning pipeline.
4. the liquid channel system of flow cytometer according to claim 3, which is characterized in that the sheath liquid device further includes sheath
Liquid filter；The sheath liquid filter is located on the sheath fluid pipeline and is located at the sheath liquid pump and the second sheath hydraulic control valve
Between, the cleaning pipeline is connected to the exhaust channel of the sheath liquid filter, is discharged in the exhaust channel of the sheath liquid filter
Irrigation source of the gas as the cleaning part.
5. the liquid channel system of flow cytometer according to any one of claims 1-4, which is characterized in that the negative pressure dress
Set the waste solution channel including being connected to the negative pressure mouth and the waste drains pump being located on the waste solution channel, waste liquid pressure sensor with
And waste liquid control valve, for the waste liquid control valve close to the flow chamber, the waste solution channel is also connected to the liquid outlet, described
Waste liquid control valve is in parallel with the emptying control valve.
6. the liquid channel system of flow cytometer according to claim 5, which is characterized in that the negative pressure device further includes giving up
Liquid container；The waste liquid pool is connected to the waste solution channel, and the waste liquid pressure sensor is located on the waste liquid pool.
7. the liquid channel system of flow cytometer according to claim 6, which is characterized in that the negative pressure device further includes giving up
Liquid pool；The waste liquid pool is connected to the waste solution channel, and the waste liquid pressure sensor is located on the waste liquid pool, the waste liquid pool
The waste fluid container is connected to by the waste solution channel, the waste drains pump.
8. a kind of application method of the liquid channel system of flow cytometer described in claim 1-7 any one, which is characterized in that
Include the following steps:
When the liquid channel system of flow cytometer is in the test of absolute counting mode, quantitative part inhales the sample liquid of predetermined volume
Enter to detect in pipeline；Then quantitative part pushes the sample in detection pipeline to enter flow chamber, while sheath fluid enters flow chamber, sample
This liquid forms stable sample flow under the package of sheath fluid and is tested by the measurement part of the flow chamber, useless after measurement
Liquid discharge；If after the sample liquid in the quantitative part is tested, the stopping test condition not up to setting then opens sample control
Valve processed, sample liquid continue to test by the measurement part that the sample unit, the sample control valve enter the flow chamber.
9. a kind of flow cytometer, which is characterized in that wanted including optical system, electronic system, testing and analysis system and right
Seek liquid fluid system described in 1-7 any one；
The optical system be connected to the flow chamber of the liquid fluid system with for irradiating the indoor sample liquid of the flowing so that
It obtains after the cell that specific fluorescence dyes is stimulated and issues fluorescence；The electronic system is used to the fluorescence signal being converted to telecommunications
Number and amplify, the testing and analysis system connects the electronic system to complete for obtaining the amplified electric signal pair
The analysis of the cell of specific fluorescence dyeing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112858661A (en) * | 2019-11-28 | 2021-05-28 | 深圳市帝迈生物技术有限公司 | Sample detection system and sample analyzer |
| CN115078324A (en) * | 2022-06-30 | 2022-09-20 | 嘉兴市唯真生物科技有限公司 | High-flux flow type fluorescence detection method, intelligent terminal and storage medium |
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