CN115078324A - High-flux flow type fluorescence detection method, intelligent terminal and storage medium - Google Patents
High-flux flow type fluorescence detection method, intelligent terminal and storage medium Download PDFInfo
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- CN115078324A CN115078324A CN202210765421.8A CN202210765421A CN115078324A CN 115078324 A CN115078324 A CN 115078324A CN 202210765421 A CN202210765421 A CN 202210765421A CN 115078324 A CN115078324 A CN 115078324A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0193—Arrangements or apparatus for facilitating the optical investigation the sample being taken from a stream or flow to the measurement cell
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides a high-throughput flow-type fluorescence detection method, an intelligent terminal and a computer readable storage medium, wherein the method comprises the following steps: pressurizing a fluid path system of the flow fluorescence detection system to enable a flow chamber in the fluid path system to have a sheath fluid flow with stable flow; sequentially scheduling a plurality of different samples to be detected to enter the flow chamber for detection; and cleaning and decompressing the liquid path system. According to the invention, after pressure is built on the liquid path system to enable sheath liquid flow with stable flow in the flow chamber, a plurality of samples to be detected are continuously scheduled to the flow chamber for detection, and finally, the liquid path system is cleaned and decompressed after all samples to be detected are detected, so that the pressure building and decompression on the liquid path system are avoided when one sample is tested, the detection efficiency is improved, and the consumption of the sheath liquid is reduced.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a high-throughput flow type fluorescence detection method, an intelligent terminal and a computer readable storage medium.
Background
In modern medical clinical examination and research laboratories, flow-type fluorescence detection systems are composed of a fluid path system, an optical detection system and a control system. The control system carries out logic control and information transmission on the components of the liquid path system and the optical detection system. In the flow fluorescence detection system, in order to ensure the stability of the test result, the stability of the sheath fluid flow rate must be ensured preferentially, and because the sheath fluid pump has periodic high-frequency fluctuation during sheath fluid conveying, a flow filter is generally added into a pipeline to relieve the high-frequency fluctuation of the flow rate. However, the addition of a flow filter increases the time taken for the sheath pump to start up until the flow stabilizes, due to the flexibility of the flow filter.
Currently, in the existing flow-type fluorescence detection system, a periodic serial control logic is adopted for testing each sample, that is, each sample needs to go through the process of "starting-sample analysis-stopping pressure release" of a sheath fluid pump. That is, each time a sample is tested, the system can analyze the sample after the sheath fluid pump is started and the flow rate is stable; moreover, in order to ensure that the pressure of each sample before the analysis test is consistent, after the previous sample is analyzed, the pressure in the sheath fluid pipeline needs to be released to return to the atmospheric pressure, so that the next sample cannot be affected. However, with the above-mentioned periodic serial control logic, in the testing process of each sample, the influence of the response time of the sheath fluid needs to be taken into account, the response time is too long, the testing efficiency is reduced, the improvement of the testing flux is restricted, and the consumption of the sheath fluid consumables is increased. Based on the technical problems, the invention provides a control mechanism of a flow-type fluorescence detection sheath liquid pump, which avoids frequent starting and stopping of the sheath liquid pump, eliminates the influence of the response time of the sheath liquid, improves the test flux and reduces the consumption rate of sheath liquid consumables.
Accordingly, the prior art is yet to be improved and developed.
Disclosure of Invention
In view of the above-mentioned shortcomings of the prior art, an object of the present invention is to provide a high-throughput flow-type fluorescence detection method, a detection system and a computer-readable storage medium, which are used to solve the problems of low test efficiency and increased sheath fluid consumption caused by frequent starting and stopping of a sheath fluid pump when a plurality of samples to be tested are tested in a flow-type fluorescence detection system in the prior art.
The technical scheme of the invention is as follows:
a high throughput flow-through fluorescence detection method, the method comprising the steps of:
pressurizing a fluid path system of the flow fluorescence detection system to enable a flow chamber in the fluid path system to have a sheath fluid flow with stable flow;
sequentially scheduling a plurality of different samples to be detected to enter the flow chamber for detection;
and cleaning and decompressing the liquid path system.
The high-flux flow-type fluorescence detection method, wherein the step of pressurizing the fluid path system to enable a flow chamber in the fluid path system to have a flow-stable sheath fluid flow comprises the following steps:
starting a sheath fluid pump of the fluid path system to pressurize the fluid path system and pump sheath fluid into the flow chamber;
when the flow rate of the sheath liquid flow in the flow chamber is stable and reaches a preset flow rate, the pressure of the liquid path system is kept unchanged.
The high-flux flow type fluorescence detection method comprises the following steps of when the flow rate of a sheath liquid flow in the flow chamber is stable and reaches a preset flow rate, keeping the pressure of the liquid path system unchanged: the preset flow range is 5ml/min-10 ml/min.
The high-flux flow-type fluorescence detection method is characterized in that the pressurization range of the liquid path system is 50-200 kPa.
The high-flux flow-type fluorescence detection method comprises the following steps of sequentially scheduling a plurality of different samples to be detected to enter the flow chamber for detection:
controlling a sample needle to sequentially obtain a plurality of samples to be detected to the flow chamber;
controlling an optical detection system of the flow type fluorescence detection system to respectively detect sample particles of each sample to be detected flowing through the flow chamber;
and acquiring signal detection data of the optical system, and calculating to generate a detection result.
The high-flux flow-type fluorescence detection method comprises the following steps of controlling the optical detection system to respectively detect sample particles of each sample to be detected flowing through the flow chamber:
controlling a laser lamp to irradiate sample particles of a sample to be detected;
and controlling the photodiode to receive scattered light signals of the sample particles and controlling the photomultiplier to receive fluorescence signals of the sample particles.
The high-throughput flow-type fluorescence detection method further comprises the following steps after the steps of acquiring signal detection data of the optical system and calculating and generating a detection result: and displaying the detection result.
The high-flux flow type fluorescence detection method comprises the following steps of:
draining the sheath fluid to the part to be cleaned so as to clean the part to be cleaned;
closing a sheath fluid pump of the fluid path system to release pressure to the fluid path system.
An intelligent terminal, the intelligent terminal comprising: a memory, a processor, and a computer readable program stored on the memory and executable on the processor; the processor, when executing the computer readable program, implements the steps in the high throughput flow-through fluorescence detection method as described in any one of the above.
A computer readable storage medium storing one or more programs, the one or more programs being executable by one or more processors to perform the steps of the high throughput streaming fluorescence detection method of any of the above.
Has the advantages that: according to the invention, after pressure is built on the liquid path system to enable sheath liquid flow with stable flow in the flow chamber, a plurality of samples to be detected are continuously scheduled to the flow chamber for detection, and finally, the liquid path system is cleaned and decompressed after all samples to be detected are detected, so that the pressure building and decompression on the liquid path system are avoided when one sample is tested, the detection efficiency is improved, and the consumption of the sheath liquid is reduced.
Drawings
FIG. 1 is a flow chart of a high-throughput flow-type fluorescence detection method according to the present invention;
fig. 2 is a schematic structural diagram of an intelligent terminal provided in the present invention.
Detailed Description
The invention provides a high-throughput flow-type fluorescence detection method, an intelligent terminal and a computer readable storage medium, and the invention is further described in detail below in order to make the purpose, technical scheme and effect of the invention clearer and clearer. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
As shown in the figure, the flow chart of the preferred embodiment of the high-flux flow-type fluorescence detection method of the invention is shown, and the method comprises the following steps:
s100, pressurizing a fluid path system of the flow type fluorescence detection system to enable a flow chamber in the fluid path system to have sheath fluid flow with stable flow;
specifically, before a sample to be detected needs to be detected, a liquid path system of the flow fluorescence detection system needs to be pressurized, that is, a pressure is established in an internal liquid flow path of the liquid path system, so that sheath liquid flows stably under the action of a certain pressure, and a stable sheath liquid flow is formed in a flow chamber in the liquid path system to prepare for detecting the sample to be detected subsequently, so that the sheath liquid flow can stably wrap the sample to be detected in the flow chamber and flows; when the sample to be detected is detected, the sample to be detected passes through the middle shaft of the sheath fluid flow under the wrapping of the sheath fluid flow, and particles of the sample to be detected can only pass through the sheath fluid flow one by one.
In one embodiment, the step of pressurizing the fluid path system to provide a flow-stabilized sheath fluid flow in a flow chamber in the fluid path system comprises:
starting a sheath fluid pump of the fluid path system to pressurize the fluid path system and pump sheath fluid into the flow chamber;
when the flow rate of the sheath liquid flow in the flow chamber is stable and reaches a preset flow rate, the pressure of the liquid path system is kept unchanged.
Specifically, a sheath liquid pump is arranged in the liquid path system, one end of the sheath liquid pump is connected with a container for storing sheath liquid through a pipeline, and the other end of the sheath liquid pump is connected with a sheath liquid inflow port of the flow chamber through a pipeline. When pressure needs to be built on the liquid path system, the sheath liquid pump is started, the sheath liquid pump operates to gradually pressurize the liquid path system, sheath liquid flows to the flow chamber along the pipeline under the pressure, and when the flow rate of the sheath liquid flow in the flow chamber is stable and reaches a preset flow rate, the output power of the sheath liquid pump is kept unchanged, so that the pressure of the liquid path system is kept unchanged, and then the flow rate of the sheath liquid in the flow chamber is also kept unchanged, so that the flow chamber has stable sheath liquid flow when a sample to be detected is detected.
When the flow rate of the sheath liquid flow in the flow chamber is stable and reaches a preset flow rate, the step of keeping the pressure of the liquid path system unchanged comprises the following steps: the range of the preset flow is 5-10 ml/min; optionally, the preset flow rate is 6ml/min, so as to reduce the output power of the sheath fluid pump and reduce the energy consumption while maintaining the stable flow of the sheath fluid in the flow chamber. Accordingly, the pressure of the sheath pump for the fluid circuit system is typically in the range of 50 kPa to 200 kPa while the flow rate in the circuit system is maintained at 5ml/min to 10ml/min by the sheath pump.
S200, sequentially scheduling a plurality of different samples to be detected to enter the flow chamber for detection;
specifically, after the sheath liquid pump establishes pressure on the liquid path system and enables the flow chamber to have stable sheath liquid flow, a sample to be detected is continuously obtained and sent to the flow chamber for detection; the bottom center of the flow chamber has an inlet for the sample to be tested, so that the sample to be tested is wrapped in the central flow of the sheath fluid stream after entering the flow chamber. When a plurality of samples to be detected are scheduled for detection, the samples to be detected are scheduled and detected continuously, namely, after one sample to be detected is detected, the sheath fluid pump is not closed to release pressure to the fluorescent fluid path system and pressure is reestablished, but the next sample to be detected is directly scheduled to the flow chamber for detection, so that the time for reestablishing pressure to the fluorescent fluid path system is saved, the sheath fluid pump is prevented from being started and stopped frequently, the detection efficiency is improved, meanwhile, the consumption of sheath fluid consumables is reduced, and the sheath fluid consumption when the fluorescent fluid path system is cleaned, released and pressure is reestablished frequently is reduced. Therefore, by means of continuously scheduling a plurality of samples to be detected for detection, no matter how many samples to be detected exist, only one sheath liquid pump needs to be started, only one process of establishing pressure on the fluorescent liquid path system is needed, and only one process of releasing pressure on the fluorescent liquid path system after detection on all samples to be detected is finished is needed.
Further, the step of sequentially scheduling a plurality of different samples to be detected to enter the flow chamber for detection includes:
controlling a sample needle to sequentially obtain a plurality of samples to be detected to the flow chamber;
controlling an optical detection system of the flow type fluorescence detection system to respectively detect sample particles of each sample to be detected flowing through the flow chamber;
and acquiring signal detection data of the optical system, and calculating to generate a detection result.
Specifically, a sample to be detected is transferred into the flow chamber by being obtained through a sample needle, the terminal controls the sample needle to sequentially obtain a plurality of samples to be detected to the flow chamber, the samples to be detected flow in the center of a sheath fluid flow in the flow chamber, then the optical detection system respectively detects sample particles of each sample to be detected flowing through the flow chamber, and then signal detection data of the optical system are obtained and calculated to generate a detection result. The sample needle continuously obtains each sample to be detected and enters the flowing chamber, the optical detection system continuously detects each sample to be detected flowing through the flowing chamber, and finally, according to the signal detection data obtained by the optical system, a detection result is calculated and generated, wherein the detection result is the detection result corresponding to each sample to be detected.
Further, the optical detection system comprises a laser lamp, a photomultiplier tube and a photodiode; the step of controlling the optical detection system to respectively detect the sample particles of each sample to be detected flowing through the flow chamber comprises the following steps:
controlling the laser lamp to irradiate sample particles of a sample to be detected;
and controlling the photodiode to receive scattered light signals of the sample particles, and controlling the photomultiplier to receive fluorescence signals of the sample particles.
When a sample to be detected passes through the flow chamber, particles of the sample to be detected are wrapped in a sheath fluid flow to pass through, the laser lamp irradiates on a flow path of the sample to be detected from one side of the flow chamber, the sample particles of each sample to be detected are irradiated in sequence, and then a photodiode and a photomultiplier tube on the other side of the flow chamber receive optical signals generated by the sample particles irradiated by the laser lamp, wherein the photodiode receives scattered light signals of the sample particles, for example, bit forward scattered light signals received by the photodiode; the photomultiplier tube receives a fluorescent signal of the sample particle.
After the step of acquiring the signal detection data of the optical system and calculating to generate the detection result, the method further comprises the following steps: and displaying the detection result. And the detection result is displayed on the display screen, so that the detection result can be conveniently checked by detection personnel.
S300, cleaning and pressure relief are carried out on the liquid path system;
after all samples to be detected are detected, a liquid path system needs to be cleaned, so that the instrument is prevented from being polluted by the residual samples to be detected, and poor infection is caused with the residual samples in the next detection; after the liquid path system is cleaned, the pressure of the liquid path system can be directly released, so that sheath liquid does not flow any more.
Specifically, the cleaning and pressure releasing of the liquid path system comprises:
draining sheath fluid to a part to be cleaned, for example, a sample needle or a tube in a fluid path system;
closing a sheath fluid pump of the fluid path system to relieve pressure to the fluid path system so that sheath fluid also no longer flows.
The present invention also provides an intelligent terminal, as shown in fig. 2, which includes at least one processor (processor) 20; a display screen 21; and a memory (memory)22, and may further include a communication Interface (Communications Interface)23 and a bus 24. The processor 20, the display 21, the memory 22 and the communication interface 23 can communicate with each other through the bus 24. The display screen 21 is configured to display a user guidance interface preset in the initial setting mode. The communication interface 23 may transmit information. The processor 20 may call logic instructions in the memory 22 to perform the methods in the embodiments described above.
Furthermore, the logic instructions in the memory 22 may be implemented in software functional units and stored in a computer readable storage medium when sold or used as a stand-alone product.
The memory 22, which is a computer-readable storage medium, may be configured to store a software program, a computer-executable program, such as program instructions or modules corresponding to the methods in the embodiments of the present disclosure. The processor 30 executes the functional application and data processing, i.e. implements the method in the above-described embodiments, by executing the software program, instructions or modules stored in the memory 22.
The memory 22 may include a storage program area and a storage data area, wherein the storage program area may store an operating system, an application program required for at least one function; the storage data area may store data created according to the use of the terminal device, and the like. Further, the memory 22 may include a high speed random access memory and may also include a non-volatile memory. For example, a variety of media that can store program codes, such as a usb disk, a removable hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk, or an optical disk, may also be transient storage media.
The present invention also provides a computer readable storage medium storing one or more programs, which are executable by one or more processors to implement the steps of the high throughput flow-type fluorescence detection method according to the above embodiments.
In addition, the specific processes loaded and executed by the instruction processors in the terminal device and the storage medium are described in detail in the method, and are not stated herein.
In summary, the present invention provides a high-throughput flow-type fluorescence detection method, a detection system and a computer-readable storage medium, wherein the method comprises the steps of: pressurizing a fluid path system to enable a flow chamber in the fluid path system to have a sheath fluid flow with stable flow; sequentially scheduling a plurality of different samples to be detected to enter the flow chamber for detection; and cleaning and decompressing the liquid path system. According to the invention, after pressure is built on the liquid path system to enable sheath liquid flow with stable flow in the flow chamber, a plurality of samples to be detected are continuously scheduled to the flow chamber for detection, and finally, the liquid path system is cleaned and decompressed after all samples to be detected are detected, so that the pressure building and decompression on the liquid path system are avoided when one sample is tested, the detection efficiency is improved, and the consumption of the sheath liquid is reduced.
It is to be understood that the invention is not limited to the examples described above, but that modifications and variations may be effected thereto by those of ordinary skill in the art in light of the foregoing description, and that all such modifications and variations are intended to be within the scope of the invention as defined by the appended claims.
Claims (10)
1. A high throughput flow-through fluorescence detection method, comprising the steps of:
pressurizing a fluid path system of the flow fluorescence detection system to enable a flow chamber in the fluid path system to have a sheath fluid flow with stable flow;
sequentially scheduling a plurality of different samples to be detected to enter the flow chamber for detection;
and cleaning and decompressing the liquid path system.
2. The high-throughput flow-type fluorescence detection method of claim 1, wherein the step of pressurizing the fluid path system to provide a flow-stabilized sheath fluid flow in a flow chamber of the fluid path system comprises:
starting a sheath fluid pump of the fluid path system to pressurize the fluid path system and pump sheath fluid into the flow chamber;
when the flow rate of the sheath liquid flow in the flow chamber is stable and reaches a preset flow rate, the pressure of the liquid path system is kept unchanged.
3. The high-throughput flow-type fluorescence detection method according to claim 2, wherein in the step of maintaining the pressure of the fluid path system constant when the flow rate of the sheath fluid in the flow chamber is stable and reaches a preset flow rate: the preset flow range is 5ml/min-10 ml/min.
4. The high-throughput flow-type fluorescence detection method of claim 2, wherein the pressurization range of the fluid path system is 50 kpa to 200 kpa.
5. The high-throughput flow-type fluorescence detection method of claim 1, wherein the step of sequentially dispatching a plurality of different samples to be detected into the flow chamber for detection comprises:
controlling a sample needle to sequentially obtain a plurality of samples to be detected to the flow chamber;
controlling an optical detection system of the flow type fluorescence detection system to respectively detect sample particles of each sample to be detected flowing through the flow chamber;
and acquiring signal detection data of the optical system, and calculating to generate a detection result.
6. The high-throughput flow-type fluorescence detection method according to claim 5,
the step of controlling the optical detection system to detect the sample particles of each sample to be detected flowing through the flow chamber comprises the following steps:
controlling a laser lamp to irradiate sample particles of a sample to be detected;
and controlling the photodiode to receive scattered light signals of the sample particles and controlling the photomultiplier to receive fluorescence signals of the sample particles.
7. The high-throughput flow-type fluorescence detection method according to claim 5, wherein the steps of acquiring signal detection data of the optical system and calculating to generate a detection result further comprise: and displaying the detection result.
8. The high-throughput flow-type fluorescence detection method of claim 1, wherein the step of cleaning and depressurizing the fluid path system comprises:
draining the sheath fluid to the part to be cleaned so as to clean the part to be cleaned;
and closing a sheath fluid pump of the fluid path system to release the pressure of the fluid path system.
9. An intelligent terminal, characterized in that, intelligent terminal includes: a memory, a processor, and a computer readable program stored on the memory and executable on the processor; the processor, when executing the computer readable program, implements the steps in the high throughput flow-through fluorescence detection method of any of claims 1-8.
10. A computer-readable storage medium storing one or more programs, the one or more programs being executable by one or more processors to perform the steps of the high throughput streaming fluorescence detection method of any one of claims 1-8.
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