WO2022001370A1 - Liquid path system for sheath flow, and control method - Google Patents

Abstract

A liquid path system for sheath flow, and control method. As a sheath liquid injector (32) is controllably connected to a sheath liquid pool, a sheath liquid inlet (22) and a reaction pool (10), when a sample is cleaned, a sheath liquid is directly injected into the reaction pool (10) and a flow chamber (20) by means of a first liquid path control device, so that the sheath liquid used for cleaning does not contain the sample, and a liquid pump (50) is used for providing a negative pressure suction force, so as to provide sufficient power for the sheath liquid used for cleaning, so that after successively passing through a sample transmission pipeline (71) and a sample injector (31), the sheath liquid is discharged out of the liquid path system for sheath flow, improving the cleaning efficiency and cleaning effect of the liquid path system for sheath flow

Description

A kind of sheath flow liquid circuit system and control method technical field

The invention relates to the technical field of flow cytometry, in particular to a sheath flow liquid circuit system and a control method.

Background technique

Current flow cytometry equipment, such as mid-to-high-end blood analyzers and flow cytometers, all use the principle of flow counting to count and classify cells or particles. Pores, through optical or electrical detection, are analyzed to obtain cell or particle number, volume information, and internal characteristics. In order to make the path of cells or particles passing through the small holes more regular, the sheath fluid is wrapped around the sample to be detected and passed through the small holes in the flow chamber, so as to ensure that the cells or particles in the sample to be detected are in the middle of the small holes. The method is called sheath flow technique or flow cytometry.

After the sheath flow liquid circuit system completes the detection of the sample to be tested, it is necessary to clean the sample in the entire sheath flow liquid circuit to avoid affecting the next test result of the sample. However, the power provided by the large-displacement syringe is relatively insufficient, and the waste liquid flowing out of the flow chamber and the sample transfer tube The sheath fluid itself may contain samples, resulting in the problems of low cleaning efficiency and poor cleaning effect of the sheath fluid circuit system.

technical problem

The technical problem mainly solved by the present invention is how to clean the sheath flow liquid path system more effectively.

technical solutions

According to a first aspect, a sheath fluid circuit system includes:

Reaction pool for storing samples to be tested;

a flow chamber, used for testing the sample to be tested, comprising a sheath fluid inlet for injecting sheath fluid and a sample injection needle for injecting the sample to be detected;

A sample injector, used to drive the sample to be tested into the flow chamber through the sample injection needle;

Sheath fluid injector for driving sheath fluid into the flow chamber through the sheath fluid inlet;

The liquid pump is used to provide negative pressure suction when preparing samples so that the samples to be tested stored in the reaction pool are sucked into the sample syringe; when cleaning samples, provide negative pressure suction to make the sheath fluid in the reaction pool pass through the sample transmission pipeline and discharge after the sample syringe;

The sheath fluid injector is controllably connected to the sheath fluid pool, the sheath fluid inlet and the second interface of the reaction pool, and is used for injecting the sheath fluid into the reaction pool when preparing the sample; injecting the sheath fluid into the flow when the sample is detected chamber; when washing the sample, the sheath fluid is injected into the reaction cell and flow chamber;

The second interface of the sample injector is connected with the first interface of the reaction tank when preparing the sample and cleaning the sample; the sample injector injects the sample to be detected into the flow chamber through the sample injection needle when the sample is detected;

The first liquid circuit control device is used to control the connection between the sheath liquid injector and the second interface of the sheath liquid pool, the sheath liquid inlet and the reaction tank;

The second liquid circuit control device is used to control the connection between the liquid pump and the first interface of the sample injector and the connection between the first interface of the reaction cell and the second interface of the sample injector when preparing the sample and cleaning the sample.

Further, the second liquid circuit control device includes a first valve and a third valve;

One end of the first valve is connected to the liquid pump, and the other end of the first valve is connected to the first interface of the sample syringe;

One end of the third valve is connected to the first interface of the reaction cell, and the other end is connected to the second interface of the sample syringe.

Further, a pressure release device is also included, which is used to release the positive pressure or negative pressure in the sheath flow liquid path before preparing the sample or after cleaning the sample.

Further, the pressure release device includes a first conduction conversion unit, and the first conduction conversion unit is used for connecting the first valve with the liquid pump or connecting the first valve with the air.

Further, the first liquid circuit control device includes a second conduction conversion unit and a third conduction conversion unit;

The second conduction conversion unit is used to connect the sheath liquid syringe and the sheath liquid pool, or connect the sheath liquid syringe and one end of the third conduction conversion unit; the third conduction conversion unit is used to connect the second conduction conversion unit. One end of the unit is connected to the sheath liquid inlet, or one end of the second conduction conversion unit is connected to the second interface of the reaction tank.

Further, it also includes a second valve, one end of the second valve is connected with the flow chamber, and the other end is connected with the second interface of the reaction tank.

Further, a fourth valve is also included, one end of which is connected to the third interface of the reaction tank, and the other end is connected to the liquid pump.

Further, it also includes a controller for controlling the actions of the liquid pump, the sample injector, the sheath liquid injector, the first liquid circuit control device and the second liquid circuit control device.

According to a second aspect, an embodiment provides a sheath flow liquid path control method, comprising:

A. Sample preparation: control the pull-down of the sample syringe and the sheath fluid syringe, inject the sheath fluid into the sheath fluid syringe through the first fluid circuit control device, start the fluid pump, and pass the second fluid circuit control device to inject the sample to be tested into the sheath fluid injector. in the sample syringe;

B. Sample detection: control the sample syringe and the sheath liquid syringe to push up, close the liquid pump, and inject the sheath liquid and the sample to be detected into the flow chamber respectively through the first liquid circuit control device to form a sheath flow and a sample flow, so as to control the sample flow. particles in the detection;

C. The first cleaning sample: control the sample syringe and the sheath liquid syringe to push up, through the first liquid path control device, so that the sheath liquid is injected into the reaction tank, start the liquid pump, and pass the second liquid path control device to make the reaction tank The sheath fluid in the sample is discharged after passing through the sample transfer pipe and the sample syringe;

D. The second cleaning sample: control the sample syringe and the sheath liquid syringe to push up, through the first liquid circuit control device, so that the sheath liquid is injected into the flow chamber, the sheath liquid in the flow chamber overflows into the reaction tank, start the liquid pump, and pass The second liquid circuit control device enables the sheath liquid in the reaction tank to be discharged after passing through the sample transmission pipeline and the sample syringe.

Further, during sample preparation, before starting the liquid pump, a pressure release device is also used to release the pressure of the sheath flow liquid circuit;

After cleaning the sample, a pressure release device was also used to release the pressure of the sheath fluid circuit.

beneficial effect

According to a sheath fluid circuit system and control method according to the above-mentioned embodiment, since the sheath fluid injector is controllably connected to the sheath fluid, the sheath fluid inlet and the reaction tank, when cleaning the sample, the sheath fluid is made to pass through the first fluid circuit control device. It is directly injected into the reaction cell and flow chamber, so that the sheath fluid used for cleaning does not contain samples, and a liquid pump is used to provide negative pressure suction to provide sufficient power for the sheath fluid used for cleaning, so that it sequentially passes through the sample transmission pipeline and After the sample syringe is discharged from the sheath fluid circuit system, the cleaning efficiency and cleaning effect of the sheath fluid fluid circuit system are improved.

Description of drawings

1 is a schematic structural diagram of a sheath flow liquid circuit system according to an embodiment;

FIG. 2 is a flow chart of a method for controlling a sheath flow liquid circuit according to an embodiment.

Embodiments of the present invention

The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments have used associated similar element numbers. In the following embodiments, many details are described so that the present application can be better understood. However, those skilled in the art will readily recognize that some of the features may be omitted under different circumstances, or may be replaced by other elements, materials, and methods. In some cases, some operations related to the present application are not shown or described in the specification, in order to avoid the core part of the present application from being overwhelmed by excessive description, and for those skilled in the art, these are described in detail. The relevant operations are not necessary, and they can fully understand the relevant operations according to the descriptions in the specification and general technical knowledge in the field.

Additionally, the features, acts, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can also be exchanged or adjusted in order in a manner obvious to those skilled in the art. Therefore, the various sequences in the specification and drawings are only for the purpose of clearly describing a certain embodiment and are not meant to be a necessary order unless otherwise stated, a certain order must be followed.

The serial numbers themselves, such as "first", "second", etc., for the components herein are only used to distinguish the described objects, and do not have any order or technical meaning. The "connection" and "connection" mentioned in this application, unless otherwise specified, include both direct and indirect connections (connections).

Example 1:

Please refer to FIG. 1. FIG. 1 is a schematic structural diagram of a sheath fluid circuit system according to an embodiment. The sheath fluid fluid circuit system includes: a reaction cell 10, a flow chamber 20, a sample syringe 31, a sheath fluid injector 32, a liquid The pump 50 , the first liquid circuit control device, the second liquid circuit control device, the sample transfer pipe 71 and the waste liquid transfer pipe 72 .

The reaction cell 10 is used for storing samples to be tested. The reaction cell 10 in this embodiment has at least three ports, which are a first port 11 connected to the sample transfer pipe 71 , a second port 12 connected to the waste liquid transfer pipe 72 , and a third port for discharging residual waste liquid. interface 13.

The flow chamber 20 is used for testing the sample to be tested, and it has at least three ports, namely, the sheath fluid inlet 22 for injecting sheath fluid, and the port for entering the sample to be tested into the flow chamber 20, that is, the sample injection needle 21 and a waste liquid outlet 23 for the outflow of waste liquid formed after detection, wherein the sheath liquid inlet 22 is located at the side of the flow chamber 20, which supplies the sheath liquid into the flow chamber 20 to form a sheath flow, and the sample injection needle 21 is located in the flow chamber 20. At the bottom, the waste liquid outlet 23 is located at the top of the flow chamber 20. The flow chamber 20 has a small hole for the samples to pass through in a queue. After the sheath liquid and the sample pass through the small hole of the flow chamber 20, they are discharged to the reaction through the waste liquid outlet 23 of the flow chamber 20. Pool 10.

The sample injector 31 is used to drive the sample to be detected stored in the reaction cell 10 to be injected into the flow chamber 20 through the sample injection needle 21 to form a sample flow, and the sheath fluid injector 32 is used to drive the sheath fluid to be injected into the flow chamber 20 through the sheath fluid inlet 22 A sheath flow is formed, and the particles or cells in the sample are encapsulated by the sheath flow in the flow chamber, so that the particles or cells can pass through the small hole in the middle position of the small hole. In order to ensure that the sample syringe 31 and the sheath liquid syringe 32 can be pushed up and down at the same time, in this embodiment, the bottoms of the push plugs of the sample syringe 31 and the sheath liquid syringe 32 are connected, such as the double syringe 30, to ensure that the two can be simultaneously pushed up and down. Pull down and push up, in another specific embodiment, the push plugs of the sample syringe 31 and the sheath fluid syringe 32 can be controlled independently of each other, and the controller uses the same speed to simultaneously control the push plugs of the syringe to pull down and push up push. In this embodiment, the capacity of the sheath fluid syringe 32 is larger than that of the sample syringe 31 .

The liquid pump 50 is used to provide negative pressure suction to make the sample to be tested stored in the reaction cell 10 be sucked into the sample syringe 31 when preparing the sample; to provide negative pressure suction to make the sheath liquid in the reaction cell 10 pass through the sample syringe 31 when cleaning the sample The sample transfer line 71 and the sample syringe 31 are then discharged.

The sheath fluid injector 32 is controllably connected to the sheath fluid reservoir, the sheath fluid inlet 22 and the second port 12 of the reaction reservoir 10, and is used to pass the sheath fluid in the sheath fluid reservoir through the second interface 12 of the reaction reservoir 10 when preparing the sample. The interface 12 is injected into the reaction cell 10; when testing samples, the sheath liquid in the sheath liquid tank is injected into the flow chamber 20 through the sheath liquid inlet 22; when cleaning the sample, the sheath liquid is injected into the reaction cell 10 and the flow chamber 20 respectively. The sheath fluid in this embodiment is provided by a sheath fluid pool in which clean sheath fluid is stored.

The sample syringe 31 in this embodiment has at least two ports, which are the second port 312 and the first port 311 respectively. The second interface 312 of the sample injector 31 is connected to the first interface 11 of the reaction cell 10 when preparing and cleaning the sample; the sample injector 31 pushes the sample to be detected into the flow chamber 20 through the sample injection needle 21 when detecting the sample.

In this embodiment, the sheath liquid injector 32 is controlled to be connected to one of the sheath liquid pool, the sheath liquid inlet 22 and the second interface 12 of the reaction cell 10 through the first liquid path control device, and the second liquid path control device is used for sample preparation and cleaning. During sampling, the control liquid pump 50 is connected to the first port 311 of the sample syringe 31 , and the first port 11 of the reaction cell 10 is connected to the second port 312 of the sample syringe 31 .

In this embodiment, the detection of the sample to be detected by the sheath flow liquid circuit system includes three stages, namely preparing the sample, testing the sample and cleaning the sample.

When preparing the sample, the sample syringe 31 and the sheath fluid syringe 32 are in the pull-down state. At this time, the liquid pump 50 is connected to the first port 311 of the sample syringe 31 and the first port of the reaction cell 10 through the second liquid circuit control device. 11 is connected to the second interface 312 of the sample syringe 31. After starting the liquid pump 50, the negative pressure suction provided by the liquid pump 50 injects the sample to be detected stored in the reaction cell 10 into the sample syringe 31 and the sample transmission pipeline 71 in the pull-down state. . At the same time, the sheath fluid injector 32 is connected to the sheath fluid pool through the first fluid path control device. At this time, since the sheath fluid injector 32 is in a pull-down state, the sheath fluid flows into the sheath fluid injector 32 .

When the sample is detected, the sample syringe 31 and the sheath fluid injector 32 are controlled to be pushed up. At this time, the sheath fluid injector 32 is connected to the sheath fluid inlet 22 through the first fluid circuit control device. As the sheath fluid injector 32 enters the sample injector 31 Push up the plug, the sheath fluid in the sheath fluid syringe 32 is injected into the flow chamber 20 through the sheath fluid inlet 22, the sample to be detected in the sample syringe 31 is injected into the flow chamber 20 through the sample injection needle 21 for detection, and the flow The waste liquid formed after the detection in the chamber 20 flows into the reaction tank 10 through the waste liquid outlet 23 and the waste liquid transmission pipeline 72 .

When cleaning the sample, the sample syringe 31 and the sheath fluid injector 32 are controlled to continue to push up, and the sheath fluid injector 32 is connected to the second interface 12 of the reaction cell 10 through the first fluid circuit control device. The liquid is injected into the reaction cell 10 through the second port 12 of the reaction cell 10, and then passes through the second liquid circuit control device, so that the liquid pump 50 is connected to the first port 311 of the sample syringe 31, and the first port 11 of the reaction cell 10 is connected to the first port 311 of the sample syringe 31. The second interface 312 of the sample syringe 31 is connected, and the newly injected sheath liquid in the reaction cell 10 is discharged through the first interface 311 of the sample syringe 31 through the sample transmission pipeline 71 and the sample syringe 31 through the negative pressure suction provided by the liquid pump 50; Then, on the basis that the sample syringe 31 and the sheath fluid injector 32 continue to be pushed up, the sheath fluid injector 32 is connected to the sheath fluid inlet 22 of the flow chamber 20 through the first fluid circuit control device, and the sheath fluid in the sheath fluid injector 32 passes through The sheath liquid inlet 22 is injected into the flow chamber 20, and then flows into the reaction tank 10 through the second interface 12 of the flow chamber 20 and the waste liquid transmission pipeline 72, and then passes through the negative pressure suction provided by the liquid pump 50 through the sample transmission pipeline 71, the sample The syringe 31 is then discharged through the first port 311 of the sample syringe 31 . In this way, the residual samples in the flow chamber 20 , the reaction cell 10 , the sample transmission pipeline 71 and the sample injector 31 are thoroughly cleaned respectively through the above two cleaning processes.

In this embodiment, the first liquid circuit control device includes a second conduction switching unit 62 and a third conduction switching unit 64 . The second conduction conversion unit 62 is used to connect the sheath liquid syringe 32 and the sheath liquid pool, or to connect the sheath liquid syringe 32 and one end of the third conduction conversion unit 64; the third conduction conversion unit 64 is used to connect the second conduction conversion unit 64 One end of the conversion unit 62 is connected to the sheath liquid inlet 22 , or one end of the second conduction conversion unit 62 is connected to the second interface 12 of the reaction cell 10 .

In one embodiment, the second conduction conversion unit 62 and the third conduction conversion unit 64 may be three-way valves, and the through valve includes a common end, a normally open end and a normally closed end, wherein the common end, the normally open end and the normally closed end Both ends are the inflow inlet or the outflow outlet of the liquid. The common end of the second conduction conversion unit 62 is connected to the sheath fluid syringe 32 , its normally closed end is connected to the sheath fluid pool, and its normally open end is connected to the common end of the third conduction transition unit 64 , and the third conduction transition unit 64 The normally closed end of the third conduction conversion unit 64 is connected to the sheath liquid inlet 22 , and the normally open end of the third conduction conversion unit 64 is connected to the second interface 12 of the reaction tank 10 .

When the common end of the second conduction conversion unit 62 is controlled to be connected to the normally closed end, the sheath liquid syringe 32 is connected to the sheath liquid, and when the common end of the second conduction conversion unit 62 is controlled to be connected to the normally open end, and the third conduction When the common end of the conversion unit 64 is connected to the normally closed end, the sheath liquid syringe 32 is connected to the sheath liquid inlet 22, and when the common end of the second conduction conversion unit 62 is controlled to be connected to the normally open end, and the third conduction conversion unit 64 is connected to the normally open end. When the common end is connected to the normally open end, the sheath liquid syringe 32 is connected to the second port 12 of the reaction cell 10 .

In this embodiment, the second liquid circuit control device includes a first valve 63 and a third valve 66 . One end of the first valve 63 is connected to the liquid pump 50, the other end of the first valve 63 is connected to the first interface 311 of the sample syringe 31; one end of the third valve 66 is connected to the first interface 11 of the reaction cell 10, and the other end is The second interface 312 of the sample syringe 31 is connected.

When both the first valve 63 and the third valve 66 are controlled to be turned on, the liquid pump 50 is connected to the first port 311 of the sample syringe 31, and the second port 312 of the sample syringe is connected to the first port 11 of the reaction cell 10, so that the liquid pump 50 is connected to the first port 311 of the sample syringe 31. The pump 50 is in communication with the reaction cell 10 .

This embodiment also includes a second valve 65 , one end of the second valve 65 is connected to the flow chamber 20 and the other end is connected to the reaction tank 10 , the second valve 65 is used to control the on-off of the waste liquid transmission pipeline 72 .

When the detection starts or ends, there will be some residual positive and negative pressures in the sheath flow liquid circuit system. The existence of these residual positive and negative pressures will cause bubbles to be generated in the sheath flow liquid circuit, which will cause the detection of cells or particles in the sample to be tested. Therefore, this embodiment further includes a pressure release device for releasing the positive or negative pressure in the sheath flow fluid path before preparing the sample or after cleaning the sample. It includes a first conduction conversion unit 61 for connecting the first valve 63 with the liquid pump 50 or connecting the first valve 63 with the air.

In one embodiment, the first conduction conversion unit 61 may be a three-way valve, which includes a common end, a normally open end and a normally closed end, and the common end of the first conduction conversion unit 61 is connected to one end of the first valve 63 , The normally closed end of the first conduction conversion unit 61 is connected to the liquid pump 50 , and the normally open end of the first conduction conversion unit 61 is connected to the air. When the common end of the first conduction conversion unit 61 is controlled to be connected to the normally open end, while the first valve 63 is turned on, the sheath flow liquid path is connected to the air, and the positive and negative pressures therein can be released.

After the above-mentioned two cleanings are performed on the entire sheath flow liquid path, some sheath liquid for cleaning will remain in the reaction tank 10. At this time, the fourth valve 67 connected to the third interface 13 of the reaction tank 10 is controlled to be turned on, Since the fourth valve 67 is also connected to the liquid pump 50, the negative pressure suction provided by the liquid pump 50 completely discharges the remaining sheath liquid in the reaction tank 10.

This embodiment further includes a controller for controlling the actions of the above-mentioned liquid pump 50 , the sample syringe 31 , the sheath liquid syringe 32 , the first liquid path control device, the second liquid path control device and the respective valves.

It should be noted that, the "connection" in the above embodiments may be a liquid path connection or a communication of liquid paths. In addition, the ends of the above-mentioned valves may be an inlet for liquid inflow or an outlet for liquid outflow.

Embodiment 2:

Please refer to FIG. 2 . FIG. 2 is a flowchart of a sheath flow liquid path control method according to an embodiment. The sheath flow liquid path control method includes the following steps:

A. Sample preparation: control the pull-down of the sample syringe 31 and the sheath fluid injector 32, through the first fluid circuit control device, so that the sheath fluid is injected into the sheath fluid injector 32, start the fluid pump, and pass through the second fluid circuit control device, so that the to-be-detected The sample is injected into the sample syringe 31 .

When preparing the sample, the sample syringe 31 and the sheath fluid syringe 32 are in the pull-down state. At this time, the liquid pump 50 is connected to the first port 311 of the sample syringe 31 and the first port of the reaction cell 10 through the second liquid circuit control device. 11 is connected to the sample syringe 31. After starting the liquid pump 50, the negative pressure suction provided by the liquid pump 50 injects the sample to be detected stored in the reaction cell 10 into the sample syringe 31 and the sample transmission pipeline 71 in the pull-down state. At the same time, the sheath fluid injector 32 is connected to the sheath fluid phase through the first fluid path control device. At this time, since the sheath fluid injector 32 is in a pull-down state, the sheath fluid flows into the sheath fluid injector 32 .

B, sample detection: control the sample injector 31 and the sheath fluid injector 32 to push up, close the liquid pump 50, and inject the sheath fluid and the sample to be detected into the flow chamber 20 through the first fluid circuit control device to form a sheath flow and a sample flow, respectively, to detect particles in the sample stream.

When the sample is detected, the sample syringe 31 and the sheath fluid injector 32 are controlled to be pushed up. At this time, the sheath fluid injector 32 is connected to the sheath fluid inlet 22 through the first fluid circuit control device. With the sheath fluid injector 32 and the sample injector 31 The middle push plug is pushed up, the sheath fluid in the sheath fluid syringe 32 is injected into the flow chamber 20 through the sheath fluid inlet 22, and the sample to be detected in the sample syringe 31 is injected into the flow chamber 20 through the sample injection needle 21 for detection, The waste liquid formed after the detection in the flow chamber 20 flows into the reaction tank 10 through the waste liquid outlet 23 and the waste liquid transmission pipeline 72 .

C. The first cleaning sample: the control sample syringe 31 and the sheath liquid syringe 32 are pushed up, and the sheath liquid is injected into the reaction tank 10 through the first liquid circuit control device, and the liquid pump 50 is activated to pass the second liquid circuit control device. , so that the sheath fluid in the reaction cell 10 is discharged after passing through the sample transmission pipe 71 and the sample syringe 31 .

D, the second cleaning sample: the control sample syringe 31 and the sheath liquid syringe 32 are pushed up, and the sheath liquid is injected into the flow chamber 20 through the first liquid circuit control device, and the sheath liquid in the flow chamber 20 overflows to the reaction tank 10, The liquid pump 50 is started, and the sheath liquid in the reaction cell 10 is discharged through the sample transmission pipe 71 and the sample syringe 31 through the second liquid circuit control device.

When cleaning the sample, control the sample syringe 31 and the sheath fluid injector 32 to continue to push up, and connect the sheath fluid injector 32 to the reaction tank 10 through the first liquid circuit control device, and the sheath fluid in the sheath fluid injector 32 passes through the reaction tank 10. The second interface 12 of the reaction tank 10 is injected into the reaction tank 10, and then the liquid pump 50 is connected to the first interface 311 of the sample syringe 31 through the second liquid circuit control device, and the first interface 11 of the reaction tank 10 is connected to the sample syringe 31. Through the negative pressure suction provided by the liquid pump 50, the newly injected sheath fluid in the reaction cell 10 is discharged through the sample transmission pipeline 71 and the sample syringe 31 through the first interface 311 of the sample syringe 31; On the basis of continuing to push up, through the first liquid circuit control device, the sheath liquid syringe 32 is connected to the sheath liquid inlet 22 of the flow chamber 20, and the sheath liquid in the sheath liquid syringe 32 is injected into the flow chamber 20 through the sheath liquid inlet 22, and then It flows into the reaction cell 10 through the waste liquid outlet 23 of the flow chamber 20 and the waste liquid transmission pipeline 72, and then passes through the sample transmission pipeline 71 and the sample syringe 31 through the negative pressure suction provided by the liquid pump, and then passes through the first interface of the sample syringe 31. 311 discharge. In this way, the residual samples in the flow chamber 20 , the reaction cell 10 , the sample transmission pipeline 71 and the sample injector 31 are thoroughly cleaned respectively through the above two cleaning processes.

During sample preparation, before starting the liquid pump 50, a pressure release device is also used to release the pressure of the sheath fluid circuit; after cleaning the sample, a pressure release device is also used to release the pressure of the sheath fluid circuit, so as to avoid the sheath fluid circuit. Air bubbles are generated due to the residual positive and negative pressure.

The specific implementations of the first liquid path control device, the second liquid path control device, and the pressure release device have been described in detail in the first embodiment, and will not be repeated here.

The above specific examples are used to illustrate the present invention, which are only used to help understand the present invention, and are not intended to limit the present invention. For those skilled in the art to which the present invention pertains, according to the idea of the present invention, several simple deductions, modifications or substitutions can also be made.

Claims (10)

1. A sheath flow liquid path system, characterized in that, comprising:

   a reaction pool (10) for storing samples to be tested;

   a flow chamber (20), used for testing the sample to be tested, comprising a sheath fluid inlet (22) for injecting sheath fluid and a sample injection needle (21) for injecting the sample to be detected;

   a sample injector (31) for driving the sample to be tested into the flow chamber (20) through the sample injection needle (21);

   a sheath fluid injector (32) for driving the sheath fluid into the flow chamber (20) through the sheath fluid inlet (22);

   The liquid pump (50) is used for providing negative pressure suction when preparing samples so that the samples to be tested stored in the reaction cell (10) are sucked into the sample syringe (31); when cleaning the samples, providing negative pressure suction to make the reaction The sheath fluid in the pool (10) is discharged through the sample transmission pipe (71) and the sample syringe (31);

   The sheath fluid injector (32) is controllably connected to the sheath fluid pool, the sheath fluid inlet (22) and the second interface (12) of the reaction pool (10), and is used for injecting the sheath fluid into the reaction pool when preparing the sample pool (10); when testing samples, the sheath fluid is injected into the flow chamber (20); when the samples are washed, the sheath fluid is injected into the reaction pool (10) and the flow chamber (20);

   The second interface (312) of the sample injector (31) is connected with the first interface (11) of the reaction cell (10) when preparing the sample and cleaning the sample; the sample injector (31) passes the sample to be detected when detecting the sample The sample injection needle (21) is injected into the flow chamber (20);

   a first liquid circuit control device for controlling the sheath liquid injector (32) to be connected to one of the sheath liquid tank, the sheath liquid inlet (22) and the second interface (12) of the reaction tank (10);

   The second liquid circuit control device is used to control the connection between the liquid pump (50) and the first interface (311) of the sample syringe (31) and the first interface (11) of the reaction cell (10) when preparing the sample and cleaning the sample Connect with the second interface (312) of the sample syringe (31).
2. The sheath flow liquid circuit system according to claim 1, wherein the second liquid path control device comprises a first valve (63) and a third valve (66);

   One end of the first valve (63) is connected to the liquid pump (50), and the other end of the first valve (63) is connected to the first interface (311) of the sample syringe (31);

   One end of the third valve (66) is connected to the first interface (11) of the reaction cell (10), and the other end is connected to the second interface (312) of the sample syringe (31).
3. The sheath fluid circuit system of claim 2, further comprising a pressure release device for releasing positive or negative pressure in the sheath fluid fluid circuit before preparing the sample or after cleaning the sample.
4. The sheath flow liquid circuit system according to claim 3, characterized in that, the pressure release device comprises a first conduction conversion unit (61), and the first conduction conversion unit (61) is used for connecting the first valve (63) communicates with the liquid pump (50) or the first valve (63) with air.
5. The sheath flow liquid path system according to claim 1, wherein the first liquid path control device comprises a second conduction conversion unit (62) and a third conduction conversion unit (64);

   The second conduction conversion unit (62) is used to connect the sheath liquid syringe (32) with the sheath liquid pool, or connect the sheath liquid syringe (32) with one end of the third conduction conversion unit (64); the third conduction conversion unit (64) The conduction conversion unit (64) is used for connecting one end of the second conduction conversion unit (62) with the sheath liquid inlet (22), or connecting one end of the second conduction conversion unit (62) with the first end of the reaction tank (10). Two interfaces (12).
6. The sheath flow liquid circuit system according to claim 1, further comprising a second valve (65), one end of the second valve is connected to the flow chamber (20), and the other end is connected to the flow chamber (20) of the reaction tank (10). The second interface is connected.
7. The sheath flow liquid circuit system according to claim 1, further comprising a fourth valve (67), one end of which is connected to the third interface of the reaction tank (10), and the other end is connected to the liquid pump (50).
8. The sheath fluid circuit system according to any one of claims 1 to 7, further comprising a controller for controlling the fluid pump (50), the sample injector (31), and the sheath fluid injector (32) , the actions of the first fluid circuit control device and the second fluid circuit control device.
9. A sheath flow liquid path control method, characterized in that, comprising:

   A. Sample preparation: control the pull-down of the sample syringe (31) and the sheath fluid injector (32), through the first fluid circuit control device, so that the sheath fluid is injected into the sheath fluid injector (32), start the fluid pump (50), and pass the first fluid circuit control device. Two-liquid circuit control device, so that the sample to be tested is injected into the sample syringe (31);

   B. Sample detection: control the sample injector (31) and the sheath fluid injector (32) to be pushed up, close the liquid pump (50), and inject the sheath fluid and the sample to be detected into the flow chamber (20) through the first fluid circuit control device, respectively. A sheath flow and a sample flow are formed in the sample flow to detect particles in the sample flow;

   C. The first cleaning sample: the control sample syringe (31) and the sheath liquid syringe (32) are pushed up, and the sheath liquid is injected into the reaction tank (10) through the first liquid circuit control device, the liquid pump is started, and the Two-liquid circuit control device, so that the sheath liquid in the reaction tank (10) is discharged after passing through the sample transmission pipeline (71) and the sample syringe (31);

   D. The second cleaning sample: the control sample syringe (31) and the sheath fluid injector (32) are pushed up, and the sheath fluid is injected into the flow chamber (20) through the first fluid circuit control device, and the sheath fluid in the flow chamber (20) is The sheath liquid overflows into the reaction tank (10), the liquid pump (50) is activated, and the sheath liquid in the reaction tank (10) is discharged through the sample transmission pipe (71) and the sample syringe (31) through the second liquid circuit control device .
10. The method according to claim 9, characterized in that, during sample preparation, before starting the liquid pump (50), a pressure release device is further used to release the pressure of the sheath flow liquid circuit;

    After cleaning the sample, a pressure release device was also used to release the pressure of the sheath fluid circuit.