CN106814183B - A kind of sheath stream detection system and sheath method of flow control - Google Patents
A kind of sheath stream detection system and sheath method of flow control Download PDFInfo
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- CN106814183B CN106814183B CN201611227624.2A CN201611227624A CN106814183B CN 106814183 B CN106814183 B CN 106814183B CN 201611227624 A CN201611227624 A CN 201611227624A CN 106814183 B CN106814183 B CN 106814183B
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Abstract
The invention discloses a kind of sheath stream detection system and sheath method of flow control, for FCM analysis technical field, including the first reaction tank, first sheath stream counting chamber, first sample syringe and the first sheath liquid syringe, the first sheath stream counting chamber includes the first sheath room, it dilutes liquid interface and protrudes into the indoor first sample needle of first sheath, the first sample syringe can draw liquid out of first reaction tank and be injected in the first sheath room by first sample needle, first sheath liquid syringe can inject dilution in the first sheath room through dilution liquid interface, the first sheath stream counting chamber is equipped with the first sewer pipe of the first reaction tank of access, first reaction tank is equipped with the first negative pressure evacuation port.One aspect of the present invention can have been subjected to dilution for many times, can offset dilution and be rinsed to liquid pipe to avoid the influence from waste liquid emptying module pressure to sheath stream counting chamber internal flow, the waste liquid that on the other hand detection generates.
Description
Technical field
The present invention is used for FCM analysis technical field, more particularly to a kind of sheath stream detection system and sheath stream controlling party
Method.
Background technique
Current flow cytometry detection device is substantially using flow cytometry principle to cell or other types
Particle classified or counted.Wherein, sheath Flow Technique or stream focusing technology, are the core technologies of flow cytometry detection device.
For functional perspective, sheath Flow Technique not only needs quickly to set up stable sample flow in a short time, while tying in detection
It also needs fluid pipe road and reaction tank to advance adequately after beam effectively to clean.
Existing technology is only to carry out unidirectional rush to sheath stream counting chamber and downstream pipe after sheath stream detection
It washes；Primary sample delivery process is realized to the preceding once residual solution in transfer canal after can only utilizing for sample transfer canal
Waste liquid is directly connected with waste solution channel emptying by the cleaning of body.And this design of the prior art, it is understood that there may be following problem:
1. being rinsed possibly to its inner wall from top to bottom using cleaning solution can not be under due to the design feature of reaction tank
The part that portion has liquid to cover sufficiently is cleaned；
2. sample transfer canal realizes the flushing to a preceding raffinate merely with rear primary sample delivery, it is possible to rear
Primary testing result brings pollution；
The emptying 3. waste liquid is directly connected with waste solution channel is easy by other negative pressure shadows in waste solution channel in detection process
It rings, the part is interfered to detect.
Summary of the invention
To solve the above problems, the present invention, which provides one kind, specially to recycle waste liquid, the sheath stream for avoiding waste-solution line from interfering
Detection system and sheath method of flow control.
The technical solution adopted by the present invention to solve the technical problems is: a kind of sheath stream detection system, including the first reaction
Pond, the first sheath stream counting chamber, first sample syringe and the first sheath liquid syringe, the first sheath stream counting chamber include the first sheath
Room dilutes liquid interface and protrudes into the indoor first sample needle of first sheath, and the first sample syringe can be from the first reaction
Liquid is drawn in pond and is injected in the first sheath room by first sample needle, and the first sheath liquid syringe can connect dilution through dilution
In the first sheath room of mouth injection, first sewer pipe of the first sheath stream counting chamber equipped with the first reaction tank of access, described first
Reaction tank is equipped with the first negative pressure evacuation port.
It is further used as the improvement of technical solution of the present invention, and the first sample syringe has the first main interface, described
The first reaction of first sample outlet tube and access of access first sample needle is equipped at first main interface of first sample syringe
The first sample inlet tube in pond, the first sewer pipe are equipped with third control valve, and first sample inlet tube is equipped with the 4th control valve.
It is further used as the improvement of technical solution of the present invention, further includes the first dilution flow container, the first sheath liquid syringe
Interface be equipped with the first dilution inlet tube, the first dilution outlet tube and control the first sheath liquid syringe with first dilute
Liquid inlet tube or the first control valve of the first dilution outlet tube conducting, the first dilution inlet tube are connected to the first dilution
Tank, the first dilution outlet tube are connected to dilution liquid interface.
It is further used as the improvement of technical solution of the present invention, and the first sample syringe has the first side interface, described
First dilution outlet tube is divided into the first detection dilution outlet tube of the first sheath stream counting chamber of access through the second control valve and connects
Enter the first cleaning dilution outlet tube of the first side interface.
It is further used as the improvement of technical solution of the present invention, and first control valve, the second control valve are controllable logical
Disconnected and commutation on-off reversing solenoid valve, the third control valve and the 4th control valve are the on-off electromagnetism of controllable on-off
Valve.
The invention also discloses another sheath stream detection systems, including the second reaction tank, the second sheath stream counting chamber, second
Sample injections device, the second sheath liquid syringe and third sheath liquid syringe, the second sheath stream counting chamber include interior sheath room, epitheca
Room, the interior dilution liquid interface communicated with interior sheath room, the outer dilution liquid interface communicated with epitheca room and to protrude into the interior sheath indoor
Second specimen needle, the second sample injections device can draw liquid out of second reaction tank and inject interior sheath by the second specimen needle
Interior, the second sheath liquid syringe can be by dilutions through in outer dilution liquid interface injection epitheca room, third sheath liquid syringe can will be dilute
It releases liquid to inject in interior sheath room through interior dilution liquid interface, the second sheath stream counting chamber second equipped with the second reaction tank of access is useless
Liquid pipe, second reaction tank are equipped with the second negative pressure evacuation port.
It is further used as the improvement of technical solution of the present invention, and the second sample injections device has the second main interface, described
The second reaction of the second sample outlet tube and access of the second specimen needle of access is equipped at second main interface of the second sample injections device
The second sample inlet tube in pond, the second sewer pipe are equipped with the 5th control valve, and the second sample inlet tube is equipped with the 6th control valve.
It is further used as the improvement of technical solution of the present invention, further includes the second dilution flow container, the second sheath liquid syringe
Interface be equipped with the second dilution inlet tube, the second dilution outlet tube and control the second sheath liquid syringe with second dilute
Liquid inlet tube or the 7th control valve of the second dilution outlet tube conducting, the second dilution inlet tube are connected to the second dilution
Tank, the second dilution outlet tube are connected to outer dilution liquid interface.
It is further used as the improvement of technical solution of the present invention, and the third sheath fluid syringe has third main interface and third
Side interface, the third detection dilution that dilution liquid interface in access is equipped at the third main interface of the third sheath liquid syringe go out
Liquid pipe, the second dilution outlet tube are equipped with the 8th control valve, and the second dilution outlet tube is through the 8th control valve point
Go out liquid to access the second detection dilution outlet tube of outer dilution liquid interface and accessing the second cleaning dilution of third side interface
Pipe.
It is further used as the improvement of technical solution of the present invention, and the second sample injections device has the second side interface, described
Second detection dilution outlet tube through the 9th control valve be divided into the outer dilution liquid interface of access the 4th detection dilution outlet tube and
Access the third cleaning dilution outlet tube of the second side interface.
It is further used as the improvement of technical solution of the present invention, and the third cleaning dilution outlet tube is equipped with throttling dress
It sets.
It is further used as the improvement of technical solution of the present invention, the 7th control valve, the 8th control valve and the 9th control valve
It is the on-off reversing solenoid valve of controllable on-off and commutation, the 5th control valve and the 6th control valve are controllable on-off
On-off solenoid valve.
It is further used as the improvement of technical solution of the present invention, and the diameter of plunger of the second sheath liquid syringe is greater than third sheath
The diameter of plunger of liquid syringe.
The invention also discloses a kind of sheath method of flow control, including the above-described sheath stream detection system of claim, packets
Include following steps:
S10. first sample syringe draws the sample to be measured of fully reacting in the first reaction tank, the first sheath liquid syringe
Draw dilution；
S20. sample push is entered the first sheath stream counting chamber, while first by first sample needle by first sample syringe
Dilution push is entered the first sheath stream counting chamber by dilution liquid interface by sheath liquid syringe, and carries out differential counting, waste liquid warp
First sewer pipe flows back to the first reaction tank；
S30. differential counting finishes, and empties the waste liquid in the first reaction tank by the first negative pressure evacuation port.
It is further used as the improvement of technical solution of the present invention, in step S10, the 4th control valve is opened, in the first reaction tank
The sample to be measured of fully reacting passes through suction function inspiration first sample syringe；Meanwhile the first control valve of control makes first
Sheath liquid syringe is connected with the first dilution inlet tube, and the dilution in the first dilution flow container sucks the first sheath by suction function
Liquid syringe.
It is further used as the improvement of technical solution of the present invention, in step S20, closes the 4th control valve, opens third control
The first sheath liquid syringe is connected with the first dilution outlet tube in valve, the first control valve of control, and the second control valve of control makes first
Dilution outlet tube and the first detection dilution outlet tube conducting, first sample syringe and the first sheath liquid syringe are respectively by sample
This and dilution are pushed into the first sheath stream counting chamber simultaneously, and carry out differential counting, and waste liquid flows back to first through the first sewer pipe
Reaction tank.
It is further used as the improvement of technical solution of the present invention, and in step S30, differential counting is finished, and closes third control valve,
The 4th control valve is opened, the second control valve of control makes the first dilution outlet tube and first cleans the conducting of dilution outlet tube, the
Dilution is squeezed into first sample syringe, first sample inlet tube and the first reaction tank through the first side interface by one sheath liquid syringe
In, meanwhile, the waste liquid in the first reaction tank is emptied by the first negative pressure evacuation port.
It is further used as the improvement of technical solution of the present invention, further comprising the steps of:
S40. third control valve is opened, first sample syringe squeezes into dilution, realization pair into the first sheath stream counting chamber
The cleaning of first sheath stream counting chamber and first sample outlet tube, the liquid cleaned enter the first reaction tank through the first sewer pipe；
S50. third control valve is closed, opens the 4th control valve, first sample syringe is squeezed into dilute into the first reaction tank
Liquid is released, realizes the cleaning to first sample inlet tube；
S60. the waste liquid in the first reaction tank is emptied by the first negative pressure evacuation port.
It is further used as the improvement of technical solution of the present invention, and in step S50, first sample syringe is repeatedly back and forth pulled,
Dilution is set repeatedly to pass through first sample inlet tube.
The invention also discloses another sheath method of flow control, including above-described sheath stream detection system, including with
Lower step:
A. the second sample injections device draws the sample to be measured of fully reacting in the second reaction tank, the second sheath liquid syringe
Dilution is drawn with third sheath liquid syringe；
B. sample push is entered the interior sheath room of the second sheath stream counting chamber by the second specimen needle by the second sample injections device,
Dilution push is entered the epitheca room of the second sheath stream counting chamber, third by outer dilution liquid interface by the second sheath liquid syringe simultaneously
Dilution push is entered the interior sheath room of the second sheath stream counting chamber by interior dilution liquid interface and carries out classification meter by sheath liquid syringe
Number, waste liquid flow back to the second reaction tank through the second sewer pipe；
C. differential counting finishes, and empties the waste liquid in the second reaction tank by the second negative pressure evacuation port.
It is further used as the improvement of technical solution of the present invention, in step A, opens the 6th control valve, anti-in the second reaction tank
Completely the second sample injections of suction function inspiration device should be passed through by sample to be measured；Meanwhile the 7th control valve of control makes the second sheath
Liquid syringe is connected with the second dilution inlet tube, and the dilution in the second dilution flow container sucks the second sheath fluid by suction function
Syringe.
It is further used as the improvement of technical solution of the present invention, and in step A, the 7th control valve of control makes the second sheath liquid syringe
It is connected with the second dilution outlet tube, the 8th control valve of control makes the second dilution outlet tube and second clean dilution outlet tube
Dilution is replenished in third sheath liquid syringe by conducting, the second sheath liquid syringe；
It is further used as the improvement of technical solution of the present invention, in step B, closes the 6th control valve, opens the 5th control valve,
It controls the 8th control valve the second dilution outlet tube is made to detect dilution outlet tube with second and be connected, controlling the 9th control valve makes the
Two detection dilution outlet tubes and the 4th detection dilution outlet tube conducting, the second sample injections device, the second sheath liquid syringe and
Third sheath liquid syringe respectively pushes sample and dilution into the second sheath stream counting chamber simultaneously, and carries out differential counting, gives up
Liquid flows back to the second reaction tank through the second sewer pipe.
It is further used as the improvement of technical solution of the present invention, and in step C, differential counting is finished, and closes the 5th control valve, control
Making the 9th control valve makes the second detection dilution outlet tube and third clean the conducting of dilution outlet tube, and the second sheath liquid syringe will
Dilution is squeezed into the second sample injections device and the second sheath stream counting chamber through the second side interface, and it is anti-that the liquid cleaned enters second
Ying Chi.
It is further used as the improvement of technical solution of the present invention, in step C, closes the 5th control valve, opens the 6th control valve,
Second sample injections device squeezes into dilution into the second reaction tank, realizes the cleaning to the second sample inlet tube.
It is further used as the improvement of technical solution of the present invention, and in step C, the second sample injections device is repeatedly back and forth pulled, and makes
Dilution repeatedly passes through the second sample inlet tube.
Beneficial effects of the present invention: in the present invention in entire liquid channel system, waste liquid emptying can share same in instrument rear end
One channel, since the emptying movement on other channels may can pass through waste liquid with the sheath stream channel parallel, the pressure on pipeline
Pipeline conduction, influences the stabilization fluid inside sheath stream counting chamber, influences testing result；It is first discharged into reaction tank thus, this
The advantages of sample processing, is that one side can empty influence of the module pressure to sheath stream counting chamber internal flow to avoid from waste liquid,
On the other hand the waste liquid that detection generates has been subjected to dilution for many times, can offset dilution and be rinsed to liquid pipe.
Detailed description of the invention
The present invention will be further explained below with reference to the attached drawings:
Fig. 1 is first embodiment of the invention structural schematic diagram；
Fig. 2 is second embodiment of the invention structural schematic diagram.
Specific embodiment
Referring to Fig.1, Fig. 2, that show the specific structures of the preferred embodiments of the invention.It will be detailed below this hair
Bright specific design.
Embodiment 1
Referring to Fig. 1, the present invention provides sheath stream detection systems, including the first reaction tank 11, the first sheath stream counting chamber 12,
One sample syringe 13 and the first sheath liquid syringe 14；The first sheath stream counting chamber 12 includes the first sheath room, dilution liquid interface
With protrude into the indoor first sample needle of first sheath, the first sheath stream counting chamber 12, which can pass through detector, to be realized and counts；Described
One sample syringe 13 can draw liquid out of first reaction tank 11 and be injected in the first sheath room by first sample needle；First sheath
Liquid syringe 14 can inject dilution in the first sheath room through dilution liquid interface；The first sheath stream counting chamber 12 is equipped with access
First sewer pipe 15 of the first reaction tank 11, first reaction tank 11 are equipped with the first negative pressure evacuation port 16.The first sample
Syringe 13 has the first main interface, and the of access first sample needle is equipped at the first main interface of the first sample syringe
The first sample inlet tube 18 of the first reaction tank 11 of one sample outlet tube 17 and access, the first sewer pipe 15 are controlled equipped with third
Valve 19, first sample inlet tube 18 are equipped with the 4th control valve 110.
It further include the first dilution flow container 111, the interface of the first sheath liquid syringe 14 is equipped with the first dilution feed liquor
Pipe 112, the first dilution outlet tube 113 and the first sheath liquid syringe 14 of control and the first dilution inlet tube 112 or the first
The first control valve 114 that dilution outlet tube 113 is connected, the first dilution inlet tube 112 are connected to the first dilution flow container 111,
First dilution outlet tube 113 is connected to dilution liquid interface.The first sample syringe 13 has the first side interface, and described the
One dilution outlet tube 113 divides to access the first of the first sheath stream counting chamber 12 the detection dilution and go out liquid through the second control valve 115
First cleaning dilution outlet tube 117 of the first side interface of pipe 116 and access.
Wherein, first control valve 114 and the second control valve 115 are the on-off commutation electricity of controllable on-off and commutation
Magnet valve, the third control valve 19 and the 4th control valve 110 are the on-off solenoid valve of controllable on-off.
The invention also discloses a kind of sheath method of flow control, including above-mentioned sheath stream detection system, comprising the following steps:
S10. after blood sample sufficiently reacts in the first reaction tank 11 with reagent, the 4th control valve 110 of unlatching, first
The sample to be measured of fully reacting passes through suction function inspiration first sample syringe 13 in reaction tank 11；Meanwhile control first
The first sheath liquid syringe 14 and the first dilution inlet tube 112 is connected in control valve 114, the dilution in the first dilution flow container 111
Liquid sucks the first sheath liquid syringe 14 by suction function；
S20. the 4th control valve 110 is closed, third control valve 19 is opened, the first control valve 114 of control infuses the first sheath fluid
Emitter 14 and the first dilution outlet tube 113 are connected, and the second control valve 115 of control makes the first dilution outlet tube 113 and first
It detects dilution outlet tube 116 to be connected, first sample syringe 13 and the first sheath liquid syringe 14 are respectively by sample and dilution
It is pushed simultaneously into the first sheath stream counting chamber 12, is transported by control first sample syringe 13 and 14 motor of the first sheath liquid syringe
Dynamic frequency controls the relative velocity of two liquid streams, so that liquid is laminar state, intermediate sample in the first sheath stream counting chamber 12
Liquid is wrapped up by the dilution (sheath fluid) of surrounding, so that the blood cell in sample liquid sequentially passes through optical detection zone, realizes classification
It counts, waste liquid flows back to the first reaction tank 11 through the first sewer pipe 15, since the waste liquid passes through dilution for many times, can serve as dilution
First time soaking and washing is carried out to the first reaction tank 11；
S30. differential counting finishes, and closes third control valve 19, opens the 4th control valve 110, controls the second control valve 115
The first dilution outlet tube 113 and the first cleaning dilution outlet tube 117 is connected, the first sheath liquid syringe 14 passes through dilution
First side interface is squeezed into first sample syringe 13, first sample inlet tube 18 and the first reaction tank 11, will be on pipe in line
Sample liquid pour in the first reaction tank 11, realize then flushing to first sample inlet tube passes through the first negative pressure evacuation port
Empty the waste liquid in the first reaction tank 11；
S40. third control valve 19 is opened, first sample syringe 13 squeezes into dilution into the first sheath stream counting chamber 12,
The first sheath liquid syringe 14 squeezes into dilution into the first sheath stream counting chamber 12 simultaneously, realizes to the first sheath stream counting chamber 12 and the
The cleaning of one sample outlet tube 17, the liquid cleaned enter the first reaction tank 11 through the first sewer pipe 15；
S50. third control valve 19 is closed, the 4th control valve 110 is opened, first sample syringe 13 is to the first reaction tank 11
In squeeze into dilution, realize the cleaning to first sample inlet tube 18, first sample syringe more than 13 times reciprocal to pull, and makes to dilute
Liquid repeatedly passes through first sample inlet tube 18, and the cleaning effect of sample inlet tube can be enhanced；
S60. the waste liquid in the first reaction tank 11 is emptied by the first negative pressure evacuation port.Since the first reaction tank 11 is into excessive
Secondary cleaning waste liquid (dilution of dilution for many times) is washed away, so the first reaction tank 11, which only needs to clean on a small quantity, directly to be started
Next sample reaction.
Whole system can be realized the dilution rapid recharge in sample injections device using control Vavle switching in the present invention,
Moreover, shortening cleaning time-consuming, entire testing process speed is improved.
Embodiment 2
Referring to fig. 2, the invention also discloses another sheath stream detection systems, including the second reaction tank 21, the second sheath stream
Counting chamber 22, the second sample injections device 23, the second sheath liquid syringe 24 and third sheath liquid syringe 25, the second sheath flow accounting
Room 22 include interior sheath room, epitheca room, the interior dilution liquid interface communicated with interior sheath room, the outer dilution liquid interface communicated with epitheca room and
Indoor second specimen needle of the interior sheath is protruded into, the second sample injections device 23 can draw liquid simultaneously out of second reaction tank 21
It is injected in interior sheath room by the second specimen needle, dilution can be injected epitheca room through outer dilution liquid interface by the second sheath liquid syringe 24
Interior, third sheath liquid syringe 25 can inject dilution in interior sheath room through interior dilution liquid interface, the second sheath stream counting chamber 22
It is equipped with the second sewer pipe 26 of the second reaction tank 21 of access, second reaction tank 21 is equipped with the second negative pressure evacuation port 27.Institute
The second sample injections device 23 is stated with the second main interface, access the is equipped at the second main interface of the second sample injections device 23
Second sample inlet tube 29 of the second reaction tank 21 of the second sample outlet tube 28 and access of two specimen needles, on the second sewer pipe 26
Equipped with the 5th control valve 210, the second sample inlet tube 29 is equipped with the 6th control valve 211.
It further include the second dilution flow container 212, the interface of the second sheath liquid syringe 24 is equipped with the second dilution feed liquor
Pipe 213, the second dilution outlet tube 214 and the second sheath liquid syringe 24 of control and the second dilution inlet tube 213 or the second
The 7th control valve 215 that dilution outlet tube 214 is connected, the second dilution inlet tube 213 are connected to the second dilution flow container 212,
Second dilution outlet tube 214 is connected to outer dilution liquid interface.The third sheath liquid syringe 25 has third main interface and the
Three side interfaces, the third that dilution liquid interface in access is equipped at the third main interface of the third sheath liquid syringe 25 detect dilution
Liquid outlet tube 216, the second dilution outlet tube 214 are equipped with the 8th control valve 217, the second dilution outlet tube
214 divide through the 8th control valve 217 to access the second detection dilution outlet tube 218 of outer dilution liquid interface and access third and flanking
Second cleaning dilution outlet tube 219 of mouth.
The second sample injections device 23 has the second side interface, and the second detection dilution outlet tube 218 is through the 9th
Control valve 222 divides to access the 4th detection dilution outlet tube 223 of outer dilution liquid interface and access the third of the second side interface
Clean dilution outlet tube 220.
The third cleaning dilution outlet tube 220 is equipped with throttling set 221, flows through third cleaning dilution with control
The flow rate of liquid of outlet tube 220.
7th control valve 215, the 8th control valve 217 and the 9th control valve 222 are controllable on-off and commutation
On-off reversing solenoid valve, the 5th control valve 210 and the 6th control valve 211 are the on-off solenoid valve of controllable on-off.
The diameter of plunger of the second sheath liquid syringe 24 is greater than the diameter of plunger of third sheath liquid syringe 25.This programme
In, the diameter of plunger of the second sheath liquid syringe 24 is big compared with third sheath liquid syringe 25 and the second sample injections device 23, so only needing
Lesser displacement is wanted to achieve that the supply to third sheath liquid syringe 25 and the flushing of the second sample outlet tube 28.
The invention also discloses another sheath method of flow control, including above-described sheath stream detection system, including with
Lower step:
A. the second sample injections device 23 draws the sample to be measured of fully reacting in the second reaction tank 21, the second sheath fluid note
Emitter 24 and third sheath liquid syringe 25 draw dilution, specifically, the 6th control valve 211 is opened, it is anti-in the second reaction tank 21
Completely the second sample injections of suction function inspiration device 23 should be passed through by sample to be measured；Meanwhile it controlling the 7th control valve 215 and makes the
Two sheath liquid syringes 24 and the second dilution inlet tube 213 are connected, and the dilution in the second dilution flow container 212 passes through suction function
The second sheath liquid syringe 24 is sucked, the 7th control valve 215 of control makes the second sheath liquid syringe 24 and the second dilution outlet tube 214
The second dilution outlet tube 214 and the second cleaning dilution outlet tube 219 is connected in conducting, the 8th control valve 217 of control, and second
Dilution is replenished in third sheath liquid syringe 25 by sheath liquid syringe 24；
B. sample push is entered the second sheath stream counting chamber 22 by the second specimen needle by the second sample injections device 23, simultaneously
Dilution push is entered the epitheca room of the second sheath stream counting chamber 22, third by outer dilution liquid interface by the second sheath liquid syringe 24
Dilution push is entered the interior sheath room of the second sheath stream counting chamber 22 by interior dilution liquid interface and is divided by sheath liquid syringe 25
Class counts, and waste liquid flows back to the second reaction tank 21 through the second sewer pipe 26, specifically, closing the 6th control valve 211, opens the 5th control
The second dilution outlet tube 214 and the second detection dilution outlet tube 218 is connected in valve 210 processed, the 8th control valve 217 of control,
Controlling the 9th control valve 222 is connected the second detection dilution outlet tube 218 and the 4th detection dilution outlet tube 223, and second
Sample and dilution are pushed entrance respectively by sample injections device 23, the second sheath liquid syringe 24 and third sheath liquid syringe 25 simultaneously
Second sheath stream counting chamber 22, the second sample injections device 23 push the second specimen needle that sample liquid enters the second sheath stream counting chamber 22
Interior, third sheath liquid syringe 25 pushes dilution (sheath fluid) and enters in the interior sheath room of the second sheath stream counting chamber 22, passes through control two
The same motor speed of syringe, so that sample liquid and interior sheath fluid form first layer in the interior sheath room of the second sheath stream counting chamber 22
Stream, sample liquid are wrapped up by sheath fluid, so that the haemocyte in sample liquid sequentially passes through interior sheath fluid outlet, i.e. the first detector should go out
Setting one has the jewel piece of micropore at mouthful, and the volume of haemocyte is detected according to theory of electrical impedance；The above process while the second sheath
Liquid syringe 24 is also pushing outer sheath fluid, and the liquid stream formed in the interior sheath room of the second sheath stream counting chamber 22 and outer sheath fluid are in outer sheath room
The second laminar flow of middle formation, sheath fluid and sample liquid in epitheca indoor and outdoor sheath fluid wraps up are examined by the second detector, at this for optics
Area is surveyed, carries out the optical detection of cell here.The volume information and optical parameter information matches that two detection positions are obtained,
Scatter plot is formed in two-dimensional coordinate, realizes blood cell count.The waste liquid that the process generates flows back to the through the second sewer pipe 26
Two reaction tanks 21 can serve as dilution and carry out first time immersion to the second reaction tank 21 since the waste liquid passes through dilution for many times
Cleaning；
C. differential counting finishes, and closes the 5th control valve 210, and the 9th control valve 222 of control goes out the second detection dilution
Liquid pipe 218 and third cleaning dilution outlet tube 220 are connected, and the second sheath liquid syringe 24 squeezes into dilution through the second side interface
In second sample injections device 23 and the second sample inlet tube 29, the sample liquid on pipe in line is poured in the second reaction tank 21,
Realize the flushing to the second sample inlet tube 29, the liquid cleaned enters the second reaction tank 21, empties by negative pressure；Open the
The first sheath liquid syringe 24 and the second sheath stream counting chamber 22 is connected in five control valves 210, the 7th control valve 215 of control, three notes
Emitter injects dilution to the second sheath stream counting chamber 22 simultaneously, realizes the flushing of sheath stream counting chamber；The 5th control valve 210 is closed,
Open the 6th control valve 211, the second sample injections device 23 squeezes into dilution into the second reaction tank 21, realize to the second sample into
The secondary cleaning of liquid pipe 29, the second more than 23 times of sample injections device back and forth pull, and dilution is made repeatedly to pass through the second sample inlet tube
29, the cleaning effect of all inlet tube can be enhanced, emptied by the second negative pressure evacuation port 27 useless in second reaction tank 21
Liquid.Since the second reaction tank 21 is into washing away for waste liquid (dilution of dilution for many times) is cleaned multiple times excessively, so the second reaction tank 21
Next sample reaction can directly be started by only needing to clean on a small quantity.
Equally, for whole system using control Vavle switching, the dilution that can be realized in sample injections device is quick in the present invention
Supply improves entire testing process speed moreover, shortening cleaning time-consuming.
Certainly, the invention is not limited to above embodiment, and those skilled in the art are without prejudice to originally
Equivalent deformation or replacement can be also made under the premise of spirit, these equivalent variation or replacement are all contained in the application right
It is required that in limited range.
Claims (10)
1. a kind of sheath stream detection system, it is characterised in that: including the first reaction tank, the first sheath stream counting chamber, first sample injection
Device and the first sheath liquid syringe, the first sheath stream counting chamber include the first sheath room, dilute liquid interface and protrude into first sheath
Indoor first sample needle, the first sample syringe can draw liquid out of first reaction tank and be infused by first sample needle
Enter in the first sheath room, the first sheath liquid syringe can inject dilution in the first sheath room through dilution liquid interface, first sheath stream
Counting chamber is equipped with the first sewer pipe of the first reaction tank of access, and first reaction tank is equipped with the first negative pressure evacuation port, described
First sample syringe has the first main interface, and access first sample is equipped at the first main interface of the first sample syringe
The first sample inlet tube of the first reaction tank of first sample outlet tube and access of needle, the first sewer pipe are controlled equipped with third
Valve, first sample inlet tube are equipped with the 4th control valve.
2. sheath stream detection system according to claim 1, it is characterised in that: it further include the first dilution flow container, described first
The interface of sheath liquid syringe is equipped with the first sheath liquid syringe of the first dilution inlet tube, the first dilution outlet tube and control
The first control valve be connected with the first dilution inlet tube or the first dilution outlet tube, the first dilution inlet tube are connected to
One dilution flow container, the first dilution outlet tube are connected to dilution liquid interface.
3. sheath stream detection system according to claim 2, it is characterised in that: the first sample syringe has the first side
Interface, the first detection dilution that the first dilution outlet tube is divided into the first sheath stream counting chamber of access through the second control valve go out
First cleaning dilution outlet tube of the first side interface of liquid pipe and access.
4. sheath stream detection system according to claim 3, it is characterised in that: first control valve, the second control valve are equal
For the on-off reversing solenoid valve that can control on-off and commutation, the third control valve and the 4th control valve are controllable on-off
On-off solenoid valve.
5. a kind of sheath stream detection system, it is characterised in that: including the second reaction tank, the second sheath stream counting chamber, the second sample injections
Device, the second sheath liquid syringe and third sheath liquid syringe, the second sheath stream counting chamber include interior sheath room, epitheca room and interior sheath
Interior dilution liquid interface that room communicates, the outer dilution liquid interface communicated with epitheca room and protrude into indoor second sample of the interior sheath
Needle, the second sample injections device can draw liquid out of second reaction tank and be injected in interior sheath room by the second specimen needle, the
Two sheath liquid syringes can be by dilution through in outer dilution liquid interface injection epitheca room, in third sheath liquid syringe can pass through dilution
It dilutes liquid interface to inject in interior sheath room, the second sheath stream counting chamber is equipped with the second sewer pipe of the second reaction tank of access, institute
The second reaction tank is stated equipped with the second negative pressure evacuation port, the second sample injections device has the second main interface, second sample
The second of the second reaction tank of the second sample outlet tube and access of the second specimen needle of access is equipped at second main interface of syringe
Sample inlet tube, the second sewer pipe are equipped with the 5th control valve, and the second sample inlet tube is equipped with the 6th control valve.
6. a kind of sheath method of flow control, which is characterized in that including sheath stream detection system of any of claims 1-4,
The following steps are included:
S10. first sample syringe draws the sample to be measured of fully reacting in the first reaction tank, and the first sheath liquid syringe is drawn
Dilution；
S20. sample push is entered the first sheath stream counting chamber, while the first sheath fluid by first sample needle by first sample syringe
Dilution push is entered the first sheath stream counting chamber by dilution liquid interface by syringe, and carries out differential counting, and waste liquid is through first
Sewer pipe flows back to the first reaction tank；
S30. differential counting finishes, and empties the waste liquid in the first reaction tank by the first negative pressure evacuation port.
7. sheath method of flow control according to claim 6, it is characterised in that: examined including sheath stream described in claim 3 or 4
Examining system, in step S30, differential counting is finished, and closes third control valve, opens the 4th control valve, and the second control valve of control makes
First dilution outlet tube and the first cleaning dilution outlet tube conducting, the first sheath liquid syringe is by dilution through the first side interface
It squeezes into first sample syringe, first sample inlet tube and the first reaction tank, meanwhile, pass through the first negative pressure evacuation port emptying the
Waste liquid in one reaction tank.
8. sheath method of flow control according to claim 7, it is characterised in that: examined including sheath stream described in claim 3 or 4
Examining system, further comprising the steps of:
S40. third control valve is opened, first sample syringe squeezes into dilution into the first sheath stream counting chamber, realizes to first
The cleaning of sheath stream counting chamber and first sample outlet tube, the liquid cleaned enter the first reaction tank through the first sewer pipe；
S50. third control valve to be closed, the 4th control valve is opened, first sample syringe squeezes into dilution into the first reaction tank,
Realize the cleaning to first sample inlet tube；
S60. the waste liquid in the first reaction tank is emptied by the first negative pressure evacuation port.
9. a kind of sheath method of flow control, which is characterized in that including the sheath stream detection system described in claim 5, including following step
It is rapid:
A. the second sample injections device draws the sample to be measured of fully reacting in the second reaction tank, the second sheath liquid syringe and third
Sheath liquid syringe draws dilution；
B. sample push is entered the interior sheath room of the second sheath stream counting chamber, while the by the second specimen needle by the second sample injections device
Dilution push is entered the epitheca room of the second sheath stream counting chamber, third sheath fluid note by outer dilution liquid interface by two sheath liquid syringes
Dilution push is entered the interior sheath room of the second sheath stream counting chamber by interior dilution liquid interface and carries out differential counting, waste liquid by emitter
The second reaction tank is flowed back to through the second sewer pipe；
C. differential counting finishes, and empties the waste liquid in the second reaction tank by the second negative pressure evacuation port.
10. sheath method of flow control according to claim 9, it is characterised in that: in step C, close the 5th control valve, open
6th control valve, the second sample injections device squeeze into dilution into the second reaction tank, realize the cleaning to the second sample inlet tube.
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| WO2019000459A1 (en) * | 2017-06-30 | 2019-01-03 | 深圳迈瑞生物医疗电子股份有限公司 | Liquid-supply device, sample-analyzing apparatus and liquid-supply method |
| CN107727557A (en) * | 2017-10-16 | 2018-02-23 | 广州竞天生物科技有限公司 | A kind of particle analyzer and its liquid-way system and detection control method |
| CN111751265A (en) * | 2020-06-30 | 2020-10-09 | 深圳市科曼医疗设备有限公司 | Sheath flow liquid path system and control method |
| CN114113571B (en) * | 2020-08-27 | 2023-12-15 | 深圳市帝迈生物技术有限公司 | Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system |
| CN112798345A (en) * | 2020-12-29 | 2021-05-14 | 深圳市科曼医疗设备有限公司 | Pre-dilution mode sample collection and distribution system, method and blood cell analyzer |
| CN116359524B (en) * | 2023-05-31 | 2023-09-15 | 深圳市帝迈生物技术有限公司 | Sample detection device |
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