CN208043616U - A kind of particle analyzer and its liquid channel system - Google Patents

A kind of particle analyzer and its liquid channel system Download PDF

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Publication number
CN208043616U
CN208043616U CN201721326736.3U CN201721326736U CN208043616U CN 208043616 U CN208043616 U CN 208043616U CN 201721326736 U CN201721326736 U CN 201721326736U CN 208043616 U CN208043616 U CN 208043616U
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China
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solenoid valve
sample
sheath fluid
pressure
liquid channel
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CN201721326736.3U
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Inventor
刘铁夫
史洪伟
王政良
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Beijing Langlang Biotechnology Co ltd
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Guangzhou Bitian Biology Technology Co Ltd
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Abstract

The utility model discloses a kind of particle analyzer and its liquid channel systems.The liquid channel system of the utility model includes sample loading system, flowing chamber component, negative pressure and zero-pressure formation mechanism and sheath fluid feed mechanism, sheath fluid feed mechanism is respectively connected with sample loading system and flowing chamber component, and sample loading system is connected by flowing chamber component with negative pressure and zero-pressure formation mechanism.Absolute counting and test constantly both of which may be used when detecting in the utility model public particle analyzer containing above-mentioned liquid channel system;Test constantly can be long lasting for measurement, and does not waste sample;The measurement i.e. absolute counting to particle concentration may be implemented in absolute counting pattern.The utility model public affairs draw cleaning solution by constant displacement pump, realize automatic cleaning sample pipeline to liquid channel system inside, are not necessarily to Manual-cleaning.

Description

A kind of particle analyzer and its liquid channel system
Technical field
The utility model is related to the optical analysis technique fields more particularly to a kind of particle analysis of a kind of biology and medicine Instrument and its liquid channel system.
Background technology
Particle analyzer is led for being detected and analyzed to all kinds of fine particle contents in liquid sample in medical treatment Domain, particle analyzer pass through applied to blood, body fluid and immune etc. particle analysis.The fluid path of general particle analyzer In system will through over-sampling, divide blood, reaction, sample to prepare, the flows such as light detection and cleaning.There are following three kinds of different schemes:
Scheme 1 can control detection sample flow and be formed, and constant displacement pump sucks a certain amount of sample, is then pushed into flow chamber, simultaneously Sheath liquid pump P1 and waste drains pump P2 provides sheath fluid and exhausts waste liquid, and the sample flow of sheath fluid package is formed in flow chamber, due to quantitative Pump pushes away sample volume it is found that so by detecting population and pushing away the bulking value of sample, obtain measure sample absolute counting value ( It is exactly particle number/corresponding sample volume, obtains the concentration value of specified particle in sample), have and facilitates absolute counting work( Energy.But due to must first draw sample and then push away sample, so the uptake of a sample must be determined in advance, and the absorption Amount has certain limitation, since sample length of pipe and constant displacement pump suction are limited in scope, causes sample uptake very big, So certain restrictions value sample size below can only be surveyed, cannot persistently measure.And such pattern, due to drawing sample again Sample is pushed away, it is often excessive to draw a certain amount of sample in order to avoid sample is diluted in pipeline, it is one remaining at the end of measurement Point not being pushed into flow chamber in pipeline is tested, so sample is caused to be wasted a part.In short, the first side Case can only survey certain restrictions value sample size below, and there are problems that sample waste.
Scheme 2 draws sample in such a way that negative pressure inhales sample, sample in sheath liquid pump P1 and waste drains pump P2 co- controlling flow chambers The negative pressure of this needle outlet, this negative pressure absorbing sample are wrapped to form sample flow into after flow chamber by sheath fluid.It is gone here and there in sample access Flowmeter is connect, closed-loop control is formed with sheath liquid pump P1, the flow velocity, flowmeter and sheath liquid pump closed-loop control by controlling sheath fluid inhale sample Speed, while negative pressure is quickly established by P3 to waste liquid pool, negative pressure in waste liquid pool is then accurately controlled by P2, to control sample This suction sample speed improves sample flow control stability.The speed that sample loading is measured with flowmeter, to realize that sample flow is formed While carry out absolute counting function.The shortcomings that this programme, is that the cost of flowmeter is higher, and since flowmeter is not to same This medium (water or blood sample) has different measured deviations, and measuring accuracy and consistency etc. is caused to have many problems.
Scheme 3 is in such a way that negative pressure inhales sample, using 1 sheath liquid pump and 1 waste drains pump and two pressure sensors, altogether With the negative pressure that specimen needle in control flow chamber exports, this negative pressure absorbing sample is wrapped to form sample into after flow chamber by sheath fluid Stream, fluid path design is succinct, but problem is can not to be directly realized by absolute counting, needs to use reference particle, increases the survey of user Measure cost.
Utility model content
In order to solve the above technical problems, the utility model provides a kind of particle analyzer and its liquid channel system, Ke Yigen According to needing to select test constantly pattern and absolute counting measurement pattern, under general mode using negative pressure suction sample by the way of, Ke Yichang Time test constantly, does not waste sample;Under absolute counting pattern, the absolute counting of volumetric method may be implemented;In addition, can automatically from Cleaning solution is drawn outside machine, clean and maintenance is carried out to liquid channel system inside, cleaning operation is carried out manually without user.
A kind of liquid channel system of particle analyzer of the utility model, including sample loading system, flowing chamber component, negative pressure And zero-pressure formation mechanism and sheath fluid feed mechanism, sheath fluid feed mechanism distinguish phase with sample loading system and flowing chamber component respectively Even, the sample loading system is connected by the flowing chamber component with the negative pressure and zero-pressure formation mechanism.
Preferably, the sample loading system includes suction needle, threeway, constant displacement pump, solenoid valve V06 and connecting line;Institute The one end and the solenoid valve V06 and the suction needle for stating threeway are sequentially connected, the second end of the threeway and the constant displacement pump Connection, the third end of the threeway is connect with the flowing chamber component.
Preferably, the flowing chamber component includes flow chamber and specimen needle, and the specimen needle is placed in the interior of the flow chamber Portion.
Preferably, the negative pressure and zero-pressure formation mechanism are by waste liquid pool, positive displacement pump, pressure sensor PS02 and solenoid valve V05 and V07 compositions;The pressure sensor PS02 is provided on the waste liquid pool, the positive displacement pump is connected to the waste liquid pool Bottom liquid outlet, open the solenoid valve V05, then the waste liquid in the waste liquid pool exhausted and establish negative pressure;Close institute Solenoid valve V05 is stated, V07 is opened and then establishes zero-pressure.
Preferably, the sheath fluid feed mechanism includes sheath fluid bucket, liquid pump, filter, solenoid valve V03 and pressure sensor PS01, the sheath fluid bucket, the liquid pump, the filter, the solenoid valve V03 and the pressure sensor PS01 connect successively It connects, sheath fluid is drawn from the sheath fluid bucket by the liquid pump, is passed by the filter, the solenoid valve V03 and the pressure Sensor PS01 supplies the flowing chamber component.
Preferably, the third end of the threeway in the sample loading system is via one section of tubule road and the specimen needle phase Even, the internal diameter≤0.3mm on the tubule road, length are more than 100mm.
Preferably, the export pipeline of the constant displacement pump divides two-way:One is connected to the threeway in the sample loading system; Another way setting pressure sensor PS03 detects its pressure, and cleaning solution is connected to by solenoid valve V01;The entrance of the constant displacement pump Pipeline setting pressure sensor PS03 detects its pressure, through the solenoid valve V02 connections sheath fluid feed mechanism.
Preferably, the opening of the flow chamber is connect by solenoid valve V04 with the waste liquid pool.
The utility model additionally provides a kind of particle analyzer, including above-mentioned liquid channel system.
The design liquid channel system and control system of the utility model provide two kinds by controlling liquid channel system working method Measurement pattern;A kind of test constantly is inhaled sample with negative pressure and is measured, prolonged test constantly may be implemented, wasted to sample It is few, but do not have absolute counting function;Another absolute counting measurement pattern can provide absolute counting function, but measure The finite volume of sample, and need specified before measuring.Two ways is selected for user, when user is not necessarily to absolute counting, then Test constantly pattern can be selected, so as to long lasting for measurement, not waste sample;When user needs absolute counting, Absolute counting pattern, specified measurement sample volume can be then selected then to start and measure.When user had not only needed absolute counting but also need When will be long lasting for measuring, then it can first enable absolute counting pattern and measure acquisition count results, then use test constantly again Pattern measures again.In addition, system can also draw cleaning solution from the outside automatically, cleaning dimension is carried out to liquid channel system inside Shield, cleaning operation is carried out without user manually.
Description of the drawings
Fig. 1 is the structural schematic diagram of a preferred embodiment of sample loading system;
Fig. 2 is the structural schematic diagram of a preferred embodiment of negative pressure and zero-pressure formation mechanism;
Fig. 3 is the structural schematic diagram of a preferred embodiment of sheath fluid feed mechanism;
Fig. 4 is a kind of structural representation of a preferred embodiment of the liquid channel system of particle analyzer of the utility model Figure.
Specific implementation mode
The invention will be further described in the following with reference to the drawings and specific embodiments, but not as a limitation of the invention.
Below in conjunction with attached drawing, the present invention is described in further detail.
Embodiment 1:One of sample loading system in a kind of liquid channel system of particle analyzer of the utility model is excellent Select embodiment
Fig. 1 is the structural schematic diagram of a preferred embodiment of sample loading system, as shown in Figure 1, the sample of the present embodiment This loading system includes suction needle 11, threeway 12, constant displacement pump 13, solenoid valve 06 and connecting line.One end of threeway 12 and electromagnetism Valve 06 and suction needle 11 are sequentially connected, and the second end of threeway 12 is connect with constant displacement pump 13, third end and the flow chamber group of threeway 12 Part 2 connects, and specifically, the third end of threeway 12 is connected via one section of tubule road 14 with specimen needle 21, the tubule road 14 Internal diameter≤0.3mm, length are more than 100mm.Solenoid valve 06 is open-minded, and constant displacement pump 13 can draw a certain amount of sample by suction needle 11 This is stored in by threeway 12 in pipeline, is then powered off solenoid valve 06, and sample is pushed up influent stream by constant displacement pump 13 by threeway 12 In dynamic room 20;Or solenoid valve 06 is opened, directly drawing sample by flow chamber 20 with negative pressure enters flow chamber 20, two ways Can easily it switch.
Embodiment 2:Negative pressure and zero-pressure formation mechanism in a kind of liquid channel system of particle analyzer of the utility model One preferred embodiment
Fig. 2 is the structural schematic diagram of a preferred embodiment of negative pressure and zero-pressure formation mechanism, as shown in Fig. 2, this implementation The negative pressure and zero-pressure formation mechanism of example are made of waste liquid pool 31, positive displacement pump 32, pressure sensor 33 and solenoid valve 05 and 07, wherein Positive displacement pump 32 is connected to the bottom liquid outlet of waste liquid pool 31, opens solenoid valve 05, can exhaust waste liquid in waste liquid pool 31, together When can take out negative pressure, according to the testing result start and stop of pressure sensor 33, maintain a more stable negative pressure, open solenoid valve 07 communicates with air and can form zero-pressure.
Embodiment 3:One of sheath fluid feed mechanism in a kind of liquid channel system of particle analyzer of the utility model is excellent Select embodiment
Fig. 3 is the structural schematic diagram of a preferred embodiment of sheath fluid feed mechanism, as shown in figure 3, the sheath of the present embodiment Liquid feed mechanism includes sheath fluid bucket 41, liquid pump 42, filter 43, solenoid valve 03 and pressure sensor 44, sheath fluid bucket 41, liquid pump 42, filter 43, solenoid valve 03 and pressure sensor 44 are sequentially connected, and are drawn sheath fluid from sheath fluid bucket 41 by liquid pump 42, are passed through Filter 43, solenoid valve 03 and pressure sensor 4 supply flow chamber 20, and forming sheath stream in inside wraps up sample flow.
Embodiment 4:An a kind of preferred embodiment for the liquid channel system of the particle analyzer of the utility model
Fig. 4 is a kind of structural representation of a preferred embodiment of the liquid channel system of particle analyzer of the utility model Figure, as shown in figure 4, the liquid channel system of the particle analyzer of the present embodiment includes sample loading system 1, flowing chamber component 2, negative pressure And zero-pressure formation mechanism 3 and sheath fluid feed mechanism 4, sheath fluid feed mechanism 4 are distinguished with sample loading system 1 and flowing chamber component 2 It is connected, sample loading system 1 is connected by flowing chamber component 2 with negative pressure and zero-pressure formation mechanism 3.
The sample loading system 1 of the present embodiment is as described in example 1 above, including suction needle 11, threeway 12, constant displacement pump 13, Solenoid valve 06 and connecting line.Solenoid valve 06 is open-minded, and constant displacement pump 13 can draw a certain amount of sample by suction needle 11 to be passed through Threeway 12 is stored in pipeline, is then powered off solenoid valve 06, and constant displacement pump 13 boosts sample flow chamber 20 by threeway 12 In;Or solenoid valve 06 is opened, directly drawing sample by flow chamber 20 with negative pressure enters flow chamber 20, and two ways can be square Just switching.
The flowing chamber component 2 of the present embodiment includes flow chamber 20, specimen needle 21 and one section of tubule road 14, the tubule road 14 Internal diameter is 0.3mm, and 14 internal diameter of tubule road of another embodiment is 0.2mm;Length is more than 100mm, and the specimen needle 21 is placed in stream Inside dynamic room 20.One end of threeway 12 in sample loading system 1 is connected with the tubule road 14 in flowing chamber component 2.
The negative pressure and zero-pressure formation mechanism 3 of the present embodiment are as described in example 2 above, by waste liquid pool 31, positive displacement pump 32, pressure Sensor 33 and solenoid valve 05 form, and wherein positive displacement pump 32 is connected to the bottom liquid outlet of waste liquid pool 31, open solenoid valve 05, Waste liquid in waste liquid pool 31 can be exhausted, while negative pressure can be taken out, according to the testing result start and stop of pressure sensor 33, maintain one A more stable negative pressure;Solenoid valve V07 is opened, then is zero-pressure in waste liquid pool 31.
The sheath fluid feed mechanism 4 of the present embodiment is as described in example 3 above, including sheath fluid bucket 41, liquid pump 42, filter 43, Solenoid valve 03 and pressure sensor 44, sheath fluid bucket 41, liquid pump 42, filter 43, solenoid valve 03 and pressure sensor 44 connect successively It connects, sheath fluid is drawn from sheath fluid bucket 41 by liquid pump 42, by filter 43, solenoid valve 03 and pressure sensor 4, supplies flow chamber 20, it forms sheath stream in inside and wraps up sample flow.
The export pipeline of constant displacement pump divides two-way in sample loading system 1:One is connected to the threeway in sample loading system 1 12;Another way setting pressure sensor PS03 detects its pressure, and cleaning solution is connected to by solenoid valve V01;Constant displacement pump 13 enters Mouth pipeline setting pressure sensor PS03 detects its pressure, through solenoid valve V02 connection sheath fluids feed mechanism 4.
The opening of flow chamber 20 in flowing chamber component 2 is connect with the waste liquid pool 31 in negative pressure and zero-pressure formation mechanism 3.
Embodiment 5:Application containing the particle analyzer of liquid channel system described in embodiment 4
The particle analyzer of the present embodiment includes the liquid channel system in embodiment 4, using the particle analyzer of the present embodiment, Including following two detection patterns and pipeline self-cleaning pattern:
Absolute counting pattern:
S1:The negative pressure and zero-pressure formation mechanism are controlled, forms zero-pressure or negative pressure in the waste liquid pool;
S2:The solenoid valve V06 is opened, the constant displacement pump draws sample via the suction needle, and sample passes through described three It is logical to be stored in the connecting line, it is then shut off the solenoid valve V06;
S3:Start the liquid pump, draws sheath fluid, the solenoid valve V03 and the solenoid valve V04 are opened simultaneously, by sheath fluid It supplies the flow chamber and enters the waste liquid pool;
S4:The sample in the connecting line is pushed to flow into the flow chamber using the constant displacement pump, in the flowing The sample flow of indoor formation sheath fluid package;
S5:Measurement is detected to the particle in the indoor sample flow of flowing, until terminating.
Test constantly pattern:
S1:The negative pressure and zero-pressure formation mechanism are controlled, negative pressure is formed in the waste liquid pool;
S2:The solenoid valve V04 and V06 is opened, sample is drawn and enters the stream via the suction needle and the threeway Dynamic room;
S3:Start the liquid pump, draws sheath fluid, open simultaneously the solenoid valve V03, sheath fluid is fed into the flowing Room, sheath fluid includes that sample forms sample flow in the flow chamber, finally enters the waste liquid pool together;
S4:The particle in sample flow in the flow chamber is detected, is terminated until measuring;
Pipeline method for self-cleaning:
S1:The solenoid valve V01 is opened, the constant displacement pump draws cleaning solution, and is stored in pipeline;
S2:The solenoid valve V01 is closed, the constant displacement pump push cleaning solution enters the suction needle and the connecting line And in the flow chamber;
S3:Soaking and washing is carried out to the connecting line and the flow chamber, to realize the cleaning function of pipeline.
Particle analyzer positive displacement pump 32, liquid pump 42, filter 43, solenoid valve 03, pressure sensor 44, flow chamber 20, Under specimen needle 21, threeway 12, the cooperation of suction needle 11 and constant displacement pump 13, sample can be drawn using constant displacement pump 13, and by sample Originally it is stored in connecting line, then the sample being stored in connecting line can be pushed to by flow chamber 20 by constant displacement pump 13 Inside measure.
Under the cooperation of solenoid valve O4, waste liquid pool 31, pressure sensor 33, solenoid valve 05 and positive displacement pump 32, drain is utilized Pump 32 and solenoid valve 05 form negative pressure, and are then measured in sample inspiration flow chamber 20 by negative pressure.The present invention passes through Control liquid channel system working method, it is possible to provide two kinds of measurement patterns:A kind of test constantly is inhaled sample with negative pressure and is measured, can be with It realizes prolonged test constantly, sample is wasted less, but do not have absolute counting function, the flow of test constantly pattern is such as Under:All valves are closed, then begin to rehearse liquid pump 32 and solenoid valve 05, establishes and maintain negative pressure in waste liquid pool 31, starts liquid pump 42, opens Solenoid valve 03 and 05 is opened, solenoid valve 06 is then turned on, sample is inhaled and measures until terminating.
Another absolute counting measurement pattern can provide absolute counting function, but measure the finite volume of sample, and It needs specified before measuring;The flow of absolute counting measurement pattern is as follows:All valves are closed, then open electromagnetic valve 06, constant displacement pump 13 Suck a certain amount of sample, start liquid pump 42, opens solenoid valve 03 and 04, constant displacement pump 13 pushes sample, sample volume pushed away or by Stop, cleaning sample pipeline.
Above two mode is selected for user, when user is not necessarily to absolute counting, then can select test constantly pattern, from And sample can not be wasted long lasting for measurement;When user needs absolute counting, then absolute counting pattern is selected, specifies and survey Sample volume is measured, then starts and measures, when user had not only needed absolute counting but also needing long lasting for measurement can be selected, then may be used Acquisition count results are measured first to enable absolute counting pattern, are then measured again with general mode again, in addition, system is also Cleaning solution can be drawn from the outside automatically, clean and maintenance is carried out to liquid channel system inside;Cleaning operation is carried out manually without user.
The preferable specific implementation mode of the above, only the utility model, but the scope of protection of the utility model is not Be confined to this, any one skilled in the art in the technical scope disclosed by the present invention, skill according to the present invention The inventive concept of art scheme is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims (7)

1. a kind of liquid channel system of particle analyzer, which is characterized in that the liquid channel system includes sample loading system, flow chamber Component, negative pressure and zero-pressure formation mechanism and sheath fluid feed mechanism, the sheath fluid feed mechanism and the sample loading system and institute It states flowing chamber component to be respectively connected with, the sample loading system forms machine by the flowing chamber component and the negative pressure and zero-pressure Structure is connected;
The negative pressure and zero-pressure formation mechanism are made of waste liquid pool, positive displacement pump, pressure sensor PS02 and solenoid valve V05 and V07; The pressure sensor PS02 is provided on the waste liquid pool, the bottom liquid that the positive displacement pump is connected to the waste liquid pool goes out Mouthful, the solenoid valve V05 is opened, then the waste liquid in the waste liquid pool is exhausted and establishes negative pressure;The solenoid valve V05 is closed, It opens V07 and then establishes zero-pressure;
The sheath fluid feed mechanism includes sheath fluid bucket, liquid pump, filter, solenoid valve V03 and pressure sensor PS01, the sheath fluid Bucket, the liquid pump, the filter, the solenoid valve V03 and the pressure sensor PS01 are sequentially connected, and pass through the liquid pump Sheath fluid is drawn from the sheath fluid bucket, by the filter, the solenoid valve V03 and the pressure sensor PS01, supplies institute State flowing chamber component.
2. liquid channel system according to claim 1, which is characterized in that the sample loading system include suction needle, threeway, Constant displacement pump, solenoid valve V06 and connecting line;One end of the threeway is sequentially connected with the solenoid valve V06 and the suction needle, The second end of the threeway is connect with the constant displacement pump, and the third end of the threeway is connect with the flowing chamber component.
3. liquid channel system according to claim 2, which is characterized in that the flowing chamber component includes flow chamber and sample Needle, the specimen needle are placed in the inside of the flow chamber.
4. liquid channel system according to claim 3, which is characterized in that the third end of the threeway in the sample loading system It is connected with the specimen needle via one section of tubule road, the internal diameter≤0.3mm on the tubule road, the thin length of pipe>100mm.
5. liquid channel system according to claim 4, which is characterized in that the export pipeline of the constant displacement pump divides two-way:One The threeway being connected in the sample loading system;Another way setting pressure sensor PS03 detects its pressure, passes through solenoid valve V01 is connected to cleaning solution;The entrance pipe setting pressure sensor PS03 of the constant displacement pump detects its pressure, through solenoid valve V02 Connect the sheath fluid feed mechanism.
6. liquid channel system according to claim 4, which is characterized in that the opening of the flow chamber by solenoid valve V04 with The waste liquid pool connection.
7. a kind of particle analyzer, which is characterized in that include the liquid channel system as described in any one of claim 1-6.
CN201721326736.3U 2017-10-16 2017-10-16 A kind of particle analyzer and its liquid channel system Active CN208043616U (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107727557A (en) * 2017-10-16 2018-02-23 广州竞天生物科技有限公司 A kind of particle analyzer and its liquid-way system and detection control method
CN110118718A (en) * 2019-05-28 2019-08-13 深圳唯公生物科技有限公司 Liquid fluid system, test method, the regulation method of negative pressure device and flow cytometer
CN110118716A (en) * 2019-05-28 2019-08-13 北京唯公医疗技术有限公司 Liquid fluid system, air-tightness detection method and the flow cytometer of flow cytometer
CN110118717A (en) * 2019-05-28 2019-08-13 北京唯公医疗技术有限公司 The liquid channel system and its application method and flow cytometer of flow cytometer
CN113218822A (en) * 2021-04-29 2021-08-06 宋卓 Focusing structure, particle detection device comprising focusing structure and use method of particle detection device

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107727557A (en) * 2017-10-16 2018-02-23 广州竞天生物科技有限公司 A kind of particle analyzer and its liquid-way system and detection control method
CN110118718A (en) * 2019-05-28 2019-08-13 深圳唯公生物科技有限公司 Liquid fluid system, test method, the regulation method of negative pressure device and flow cytometer
CN110118716A (en) * 2019-05-28 2019-08-13 北京唯公医疗技术有限公司 Liquid fluid system, air-tightness detection method and the flow cytometer of flow cytometer
CN110118717A (en) * 2019-05-28 2019-08-13 北京唯公医疗技术有限公司 The liquid channel system and its application method and flow cytometer of flow cytometer
CN113218822A (en) * 2021-04-29 2021-08-06 宋卓 Focusing structure, particle detection device comprising focusing structure and use method of particle detection device

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