CN114113571B - Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system - Google Patents
Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system Download PDFInfo
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- CN114113571B CN114113571B CN202010881057.2A CN202010881057A CN114113571B CN 114113571 B CN114113571 B CN 114113571B CN 202010881057 A CN202010881057 A CN 202010881057A CN 114113571 B CN114113571 B CN 114113571B
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- 239000007788 liquid Substances 0.000 title claims abstract description 118
- 238000004140 cleaning Methods 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000003018 immunoassay Methods 0.000 title claims abstract description 14
- 239000004005 microsphere Substances 0.000 claims abstract description 17
- 239000012530 fluid Substances 0.000 claims description 65
- 239000002699 waste material Substances 0.000 claims description 23
- 238000011084 recovery Methods 0.000 claims description 20
- 238000005086 pumping Methods 0.000 claims description 19
- 238000001514 detection method Methods 0.000 claims description 15
- 238000004891 communication Methods 0.000 claims description 12
- 238000007599 discharging Methods 0.000 claims description 6
- 230000003287 optical effect Effects 0.000 claims description 6
- 238000005259 measurement Methods 0.000 claims description 2
- 230000037361 pathway Effects 0.000 claims 1
- 239000002245 particle Substances 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
Abstract
The application relates to the technical field of medical equipment, and particularly discloses an immunoassay analyzer, a liquid path system thereof and a cleaning method of the liquid path system, wherein the liquid path system comprises the following components: a flow chamber. And the cleaning liquid storage tank is communicated with the flow chamber. And the sheath liquid injector is respectively communicated with the cleaning liquid storage tank and the flow chamber, and is used for conveying the cleaning liquid in the cleaning liquid storage tank to the flow chamber, wherein the cleaning liquid is used for cleaning the inner wall of the flow chamber so as to remove the microspheres in the flow chamber. Through the mode, the application can effectively remove the residual fluorescent coding microspheres in the flow chamber, does not need a user to manually carry out cleaning operation, and reduces the risk of pollution of the sample to be tested.
Description
Technical Field
The application relates to the technical field of medical equipment, in particular to an immunoassay analyzer, a liquid path system and a cleaning method of the liquid path system.
Background
The flow type fluorescence, also called suspension array, liquid phase chip, etc., uses fluorescence encoding microsphere as core, integrates multiple technologies such as flow concentration principle, laser analysis, high-speed digital signal processing, etc., and has the characteristics of high flux, high sensitivity, parallel detection, etc.; can be used for various aspects and fields of research such as immunoassay, nucleic acid research, enzymology analysis, receptor, ligand recognition analysis and the like.
The inventors of the present application have found during long-term studies that if it is desired to increase the detection speed, the detection period is generally shortened, which also results in a shortened time for washing, and thus fluorescent-encoded microspheres are liable to remain in the flow cell, increasing the risk of contamination of the sample to be measured.
Disclosure of Invention
In order to solve the technical problems, the application provides an immunoassay analyzer, a liquid path system and a cleaning method of the liquid path system, which can clean and maintain a flow chamber, effectively remove fluorescent coding microspheres remained in the flow chamber and reduce the risk of pollution of a sample to be tested.
In order to solve the technical problems, the application adopts a technical scheme that: there is provided a liquid path system comprising: a flow chamber. And the cleaning liquid storage tank is communicated with the flow chamber. And the sheath liquid injector is respectively communicated with the cleaning liquid storage tank and the flow chamber, and is used for conveying the cleaning liquid in the cleaning liquid storage tank to the flow chamber, wherein the cleaning liquid is used for cleaning the inner wall of the flow chamber so as to remove the microspheres in the flow chamber.
In order to solve the technical problems, the application adopts another technical scheme that: an immunoassay analyzer is provided, which comprises the liquid path system and a control circuit.
In order to solve the technical problems, the application adopts another technical scheme that: there is provided a method of cleaning a liquid path system, the method being based on a liquid path system as described above, the method comprising: the sheath liquid injector is used for conveying the cleaning liquid in the cleaning liquid storage tank to the flow chamber so as to clean the flow chamber.
The beneficial effects of the application are as follows: compared with the prior art, the cleaning solution in the cleaning solution storage tank is conveyed to the flow chamber through the sheath solution injector, wherein the cleaning solution in the cleaning solution storage tank is used for cleaning the inner wall of the flow chamber, so that the fluorescent coding microspheres remained in the flow chamber are effectively removed, the cleaning operation is not required to be manually performed by a user, and the risk of pollution of a sample to be tested is reduced.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:
FIG. 1 is a schematic diagram of a fluid circuit system according to an embodiment of the present application;
FIG. 2 is a schematic diagram of another embodiment of a fluid circuit system according to the present application;
FIG. 3 is a schematic diagram of an immunoassay device according to an embodiment of the present application.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
In the application, reagents used in the immunoassay analyzer comprise one or more of fluorescent coding microspheres, antibodies and fluorescent biotin, an original sample is provided for a sample to be tested, including but not limited to whole blood, serum and the like, and the sample to be tested is a sandwich structure comprising the fluorescent coding microspheres, the antigens, the antibodies and the fluorescent biotin. Sample particles to be measured flow through the flow chamber in an arrangement mode, and the particles in the sample flow are irradiated by laser and various scattered light signals generated in irradiation are collected, and the signals are classified and counted by combining biological characteristics to obtain parameter information with clinical significance. If the washing time is insufficient, the fluorescent coding microspheres are easy to remain in the flow chamber, and the risk of pollution of the sample to be detected is increased.
In order to solve the technical problems, the application provides a liquid path system.
Referring to fig. 1, a fluid path system 10 according to an embodiment of the present application includes: a flow chamber 11, a cleaning liquid storage tank 12, and a sheath liquid syringe 13. The cleaning liquid storage tank 12 communicates with the flow chamber 11. The sheath fluid injector 13 is respectively communicated with the cleaning fluid storage tank 12 and the flow chamber 11, and the sheath fluid injector 13 is used for delivering the cleaning fluid in the cleaning fluid storage tank 12 to the flow chamber 11, wherein the cleaning fluid is used for cleaning the inner wall of the flow chamber 11 so as to remove microspheres (not shown) in the flow chamber 11.
The cleaning liquid storage tank 12 is used to supply new cleaning liquid to the flow chamber 11. That is, when the waste liquid of the flow chamber 11 is discharged, a new cleaning liquid can be further refilled into the flow chamber 11 by using the cleaning liquid storage tank 12 to perform a secondary cleaning of the flow chamber 11.
Different from the condition of the prior art, the cleaning solution in the cleaning solution storage tank 12 is conveyed to the flow chamber 11 through the sheath solution injector 13, wherein the cleaning solution in the cleaning solution storage tank 12 is used for cleaning the inner wall of the flow chamber 11 so as to remove the microspheres in the flow chamber 11, the cleaning operation is not required to be manually performed by a user, the residual fluorescent coding microspheres in the flow chamber 11 are effectively removed, and the risk of pollution of a sample to be detected is reduced.
Referring to fig. 2, in one embodiment, the fluid circuit system 10 further includes: the device comprises a first electromagnetic valve LV11 and a second electromagnetic valve LV06, wherein a first interface of the first electromagnetic valve LV11 is communicated with a sheath fluid injector 13, a second interface of the first electromagnetic valve LV11 is communicated with a first interface of the second electromagnetic valve LV06, a second interface of the second electromagnetic valve LV06 is communicated with a cleaning fluid storage tank 12, and a third interface of the second electromagnetic valve LV06 is communicated with a first interface of a flow chamber 11.
Wherein, sheath liquid injector 13 is used for carrying the pipeline between first solenoid valve LV11 and second solenoid valve LV06 with the washing liquid in washing liquid storage tank 12 when the first interface intercommunication second solenoid valve LV06 of second solenoid valve LV06, and the first interface intercommunication first solenoid valve LV11 of first solenoid valve LV 11.
The sheath fluid injector 13 is further configured to, when the third port of the second solenoid valve LV06 is connected to the first port of the second solenoid valve LV06 and the first port of the first solenoid valve LV11 is connected to the second port of the first solenoid valve LV11, transfer the cleaning fluid in the pipe between the first solenoid valve LV11 and the second solenoid valve LV06 to the flow chamber 11 to clean the flow chamber 11.
Referring to fig. 2, in one embodiment, the fluid circuit system 10 further includes: the device comprises a pumping pump 14, a buffer mechanism 15, a waste liquid recovery tank 16, a third electromagnetic valve LV07 and an eighth electromagnetic valve LV10, wherein a first interface of the third electromagnetic valve LV07 is communicated with a second interface of the flow chamber 11, a second interface of the third electromagnetic valve LV07 is communicated with a first interface of the buffer mechanism 15, a first interface of the eighth electromagnetic valve LV10 is communicated with a second interface of the buffer mechanism 15, a second interface of the eighth electromagnetic valve LV10 is communicated with the pumping pump 14, and the pumping pump 14 is communicated with the waste liquid recovery tank 16.
The pump 14 is used to discharge the liquid in the flow chamber 11 to the waste liquid recovery tank 16 when the first port of the third solenoid valve LV07 communicates with the second port of the third solenoid valve LV07, the first port of the eighth solenoid valve LV10 communicates with the second port of the eighth solenoid valve LV10, and the pump 14 is turned on.
Referring to fig. 2, in one embodiment, the fluid circuit system 10 further includes: the sheath fluid storage tank 17, the buffer mechanism 15, the third electromagnetic valve LV07, the fourth electromagnetic valve LV05, the fifth electromagnetic valve LV02 and the sixth electromagnetic valve LV04, wherein the first interface of the third electromagnetic valve LV07 is communicated with the second interface of the flow chamber 11, the second interface of the third electromagnetic valve LV07 is communicated with the first interface of the buffer mechanism 15, the first interface of the fourth electromagnetic valve LV05 is communicated with the sheath fluid injector 13, the second interface of the fourth electromagnetic valve LV05 is communicated with the first interface of the first electromagnetic valve LV11, the third interface of the fourth electromagnetic valve LV05 is respectively communicated with the first interface of the fifth electromagnetic valve LV02 and the first interface of the sixth electromagnetic valve LV04, the second interface of the fifth electromagnetic valve LV02 is communicated with the third interface of the flow chamber 11, and the second interface of the sixth electromagnetic valve LV04 is communicated with the sample injector 19.
The sheath fluid injector 13 is further configured to transfer sheath fluid in the sheath fluid storage tank 17 to a conduit between the fourth solenoid valve LV05 and the first solenoid valve LV11 when the first port of the sixth solenoid valve LV04 is not in communication with the second port of the sixth solenoid valve LV04, the first port of the fifth solenoid valve LV02 is not in communication with the second port of the fifth solenoid valve LV02, the first port of the third solenoid valve LV07 is not in communication with the second port of the third solenoid valve LV07, the first port of the fourth solenoid valve LV05 is in communication with the second port of the fourth solenoid valve LV05, and the first port of the first solenoid valve LV11 is in communication with the third port of the first solenoid valve LV 11.
The sheath fluid injector 13 is further configured to transfer sheath fluid in a conduit between the fourth solenoid valve LV05 and the first solenoid valve LV11 to the flow chamber 11 when the first port of the sixth solenoid valve LV04 communicates with the second port of the sixth solenoid valve LV04, the first port of the fifth solenoid valve LV02 communicates with the second port of the fifth solenoid valve LV02, the first port of the third solenoid valve LV07 communicates with the second port of the third solenoid valve LV07, and the first port of the fourth solenoid valve LV05 communicates with the third port of the fourth solenoid valve LV 05.
In this embodiment, the sheath fluid and the cleaning fluid may have the same composition or may have different compositions.
Referring to fig. 2, in one embodiment, the fluid circuit system 10 further includes: a sample injector 19, a measuring needle mechanism 21 and a seventh solenoid valve LV01.
The measuring needle mechanism 21 comprises a measuring needle (not shown) and a stirring piece (not shown), and the measuring needle mechanism 21 is used for sucking a sample to be detected into the optical detection mechanism for detection; the stirring piece stirs the mixture of the original sample and the fluorescent coding microsphere, so that the antigen in the original sample and the specific antibody in the fluorescent coding microsphere are fully combined.
After incubation and magnetic separation are finished, a sample to be tested is prepared. After that, the sample to be measured is sucked into the optical detection mechanism by the measuring needle mechanism 21 for detection. In addition, before the sample to be detected is sucked into the optical detection mechanism for detection, the sample to be detected is mixed uniformly, and the mixing method comprises one or more of mechanical mixing, bubble mixing or sucking and spitting mixing. In other implementations; in the example, the measuring needle mechanism 21 is configured to mix the sample in a cuvette (not shown) by sucking and discharging the sample, and thus, it is possible to avoid the separate provision of a stirring device, which is advantageous in simplifying the structure of the immunoassay analyzer.
Wherein, the first interface of the sample injector 19 is communicated with the second interface of the sixth electromagnetic valve LV04, the second interface of the sample injector 19 is respectively communicated with the fourth interface of the flow chamber 11 and the first interface of the seventh electromagnetic valve LV01, and the second interface of the seventh electromagnetic valve LV01 is communicated with the first interface of the measuring needle mechanism 21.
After the sample to be measured is uniformly mixed, the measuring needle mechanism 21 moves to the bottom of the reaction cup, at this time, the sheath injector 13 is used for conveying the sample to be measured to a pipeline between the seventh electromagnetic valve LV01 and the sample injector 19 when the first interface of the seventh electromagnetic valve LV01 is communicated with the second interface of the seventh electromagnetic valve LV01, the first interface of the sixth electromagnetic valve LV04 is communicated with the second interface of the sixth electromagnetic valve LV04, and the first interface of the fourth electromagnetic valve LV05 is communicated with the third interface of the fourth electromagnetic valve LV05, and it is required that the liquid level of the sample to be measured is just at the first interface of the seventh electromagnetic valve LV01 at this time.
The sheath fluid injector 13 is configured to deliver a sample to be measured in a pipe between the seventh solenoid valve LV01 and the sample injector 19 to the flow chamber 11 when the first port of the seventh solenoid valve LV01 is not in communication with the second port of the seventh solenoid valve LV01.
Further, the plunger of the sheath fluid syringe 13 and the plunger of the sample syringe 19 are driven by the same driving mechanism for applying a pushing or pulling force to the plunger of the sheath fluid syringe 13 and the plunger of the sample syringe 19. In this embodiment, the sheath fluid injector 13 and the sample injector 19 make the magnitudes of pressure changes in the pipeline different, the sample injector 19 performs coarse adjustment of the magnitude order of the pressure in the pipeline, the sheath fluid injector 13 performs fine adjustment of a specific value on the pressure in the pipeline, and the combination of the large-volume injector and the small-volume injector can rapidly and accurately control the magnitude of the pressure in the pipeline and the volume of the liquid conveyed in the pipeline, thereby improving the operation efficiency.
Referring to fig. 2, in one embodiment, the fluid circuit system 10 further includes: the device comprises a pumping pump 14, a waste liquid recovery tank 16 and an eighth electromagnetic valve LV10, wherein a first interface of the eighth electromagnetic valve LV10 is communicated with a second interface of a buffer mechanism 15, a second interface of the eighth electromagnetic valve LV10 is communicated with the pumping pump 14, and the pumping pump 14 is communicated with the waste liquid recovery tank 16.
The pump 14 is used to discharge the liquid in the flow chamber 11 to the waste liquid recovery tank 16 when the first port of the third solenoid valve LV07 communicates with the second port of the third solenoid valve LV07, the first port of the eighth solenoid valve LV10 communicates with the second port of the eighth solenoid valve LV10, and the pump 14 is turned on.
Further, the sheath liquid storage tank 17 and the cleaning liquid storage tank 12 are respectively provided with a liquid level detection device (not shown), and when detecting that the liquid levels of the sheath liquid storage tank 17 and the cleaning liquid storage tank 12 are lower than a preset value, an alarm is given to supplement the sheath liquid and the cleaning liquid. Of course, the waste liquid recovery tank 16 is also provided with a liquid level detection device for warning to clear the waste liquid when the liquid level of the waste liquid recovery tank 16 exceeds a preset height.
Specifically, the waste liquid recovery tank 16 is provided at the end of a drain line (not shown) through which the pump 14 is connected. Thus, the waste liquid discharged from the liquid path system 10 can be injected into the waste liquid recovery tank 16 through the relevant path. The pump 14 may be a diaphragm pump that does not require frequent replacement of the associated tubing relative to peristaltic pumps, and that has a longer life while facilitating better maintenance.
Referring to fig. 2, in one embodiment, the fluid circuit system 10 further includes: the ninth solenoid valve LV08, the first port of the ninth solenoid valve LV08 communicates with the second port of the measuring needle mechanism 21, the second port of the ninth solenoid valve LV08 communicates with the pump 14, and the pump 14 communicates with the waste liquid recovery tank 16.
The sheath liquid injector 13 is used for delivering sheath liquid in the sheath liquid injector 13 to the measuring needle mechanism 21 and discharging sheath liquid in the measuring needle mechanism 21 to the waste liquid recovery tank 16 when the first interface of the fourth electromagnetic valve LV05 is communicated with the third interface of the fourth electromagnetic valve LV05, the first interface of the sixth electromagnetic valve LV04 is communicated with the second interface of the sixth electromagnetic valve LV04, the first interface of the seventh electromagnetic valve LV01 is communicated with the second interface of the seventh electromagnetic valve LV01, the first interface of the ninth electromagnetic valve LV08 is communicated with the second interface of the ninth electromagnetic valve LV08, and the extraction pump 14 is turned on.
Referring to fig. 2, in an embodiment, the sheath injector 13 is used to transfer sheath fluid in the sheath injector to the lower port of the swab of the measuring needle mechanism and drain sheath fluid through the upper port of the swab of the measuring needle mechanism when the first port of the fourth solenoid valve LV05 is in communication with the second port of the fourth solenoid valve LV05, the first port of the ninth solenoid valve LV08 is in communication with the second port of the ninth solenoid valve LV08, and the pump 14 is on.
Referring to fig. 2, in one embodiment, the fluid circuit system 10 further includes: the first port of the tenth electromagnetic valve LV09 is communicated with the third port of the measuring needle mechanism 21, and the second port of the tenth electromagnetic valve LV09 is communicated with the first port of the sixth electromagnetic valve LV04 and the third port of the fourth electromagnetic valve LV05, respectively.
The sheath fluid injector 13 is configured to deliver sheath fluid in the sheath fluid injector 13 to the measurement needle mechanism 21 when the first port of the fourth solenoid valve LV05 is in communication with the third port of the fourth solenoid valve LV05, the first port of the sixth solenoid valve LV04 is not in communication with the second port of the sixth solenoid valve LV04, and the first port of the tenth solenoid valve LV09 is in communication with the second port of the tenth solenoid valve LV 09.
Referring to fig. 3, the present application further provides an immunoassay device 100, where the immunoassay device 100 includes a liquid path system 10, an optical detection device 20 and a control circuit 30 according to the above embodiment. In this embodiment, the optical detection device 20 includes a laser emitting device (not shown) and a beam collecting device (not shown), the sample particles to be measured flow through the flow chamber in an arrangement manner, the laser emitting device emits laser to the sample particles to be measured wrapped by the sheath liquid, and the beam collecting device collects the intensities of scattered light and fluorescence emitted by the sample particles to be measured under the irradiation of the laser, so as to further determine the classification and the number of the samples to be measured.
The application also provides a cleaning method of the liquid path system, which is based on the liquid path system of the embodiment, and comprises the following steps: the cleaning liquid in the cleaning liquid storage tank is fed to the flow chamber by the sheath liquid injector to clean the flow chamber, wherein the residence time of the cleaning liquid in the flow chamber is 3-15 minutes, for example 3 minutes, 5 minutes, 10 minutes or 15 minutes.
Compared with the prior art, the cleaning solution in the cleaning solution storage tank is conveyed to the flow chamber through the sheath solution injector, wherein the cleaning solution in the cleaning solution storage tank is used for cleaning the inner wall of the flow chamber, so that the fluorescent coding microspheres remained in the flow chamber are effectively removed, the cleaning operation is not required to be manually performed by a user, and the risk of pollution of a sample to be tested is reduced.
The foregoing description is only of embodiments of the present application, and is not intended to limit the scope of the application, and all equivalent structures or equivalent processes using the descriptions and the drawings of the present application or directly or indirectly applied to other related technical fields are included in the scope of the present application.
Claims (11)
1. A fluid path system, comprising:
a flow chamber;
a cleaning liquid storage tank in communication with the flow chamber; and
the sheath liquid injector is respectively communicated with the cleaning liquid storage tank and the flow chamber and is used for conveying the cleaning liquid in the cleaning liquid storage tank to the flow chamber, wherein the cleaning liquid is used for cleaning the inner wall of the flow chamber so as to remove microspheres in the flow chamber;
the first interface of the first electromagnetic valve is communicated with the sheath fluid injector, the second interface of the first electromagnetic valve is communicated with the first interface of the second electromagnetic valve, the second interface of the second electromagnetic valve is communicated with the cleaning fluid storage tank, and the third interface of the second electromagnetic valve is communicated with the first interface of the flow chamber;
the sheath liquid injector is used for conveying the cleaning liquid in the cleaning liquid storage tank to a pipeline between the first electromagnetic valve and the second electromagnetic valve when the first interface of the second electromagnetic valve is communicated with the second interface of the second electromagnetic valve and the first interface of the first electromagnetic valve is communicated with the second interface of the first electromagnetic valve;
the sheath liquid injector is further used for conveying the cleaning liquid in the pipeline between the first electromagnetic valve and the second electromagnetic valve to the flow chamber when the third interface of the second electromagnetic valve is communicated with the first interface of the second electromagnetic valve and the first interface of the first electromagnetic valve is communicated with the second interface of the first electromagnetic valve.
2. The fluid circuit system of claim 1, wherein the fluid circuit system further comprises: the device comprises a pumping pump, a buffer mechanism, a waste liquid recovery tank, a third electromagnetic valve and an eighth electromagnetic valve, wherein a first interface of the third electromagnetic valve is communicated with a second interface of the flow chamber, a second interface of the third electromagnetic valve is communicated with a first interface of the buffer mechanism, a first interface of the eighth electromagnetic valve is communicated with a second interface of the buffer mechanism, a second interface of the eighth electromagnetic valve is communicated with the pumping pump, and the pumping pump is communicated with the waste liquid recovery tank;
the pumping pump is used for discharging the liquid in the flowing chamber to the waste liquid recovery tank when the first interface of the third electromagnetic valve is communicated with the second interface of the third electromagnetic valve and the first interface of the eighth electromagnetic valve is communicated with the second interface of the eighth electromagnetic valve.
3. The fluid circuit system of claim 1, wherein the fluid circuit system further comprises: the device comprises a sheath fluid storage tank, a buffer mechanism, a third electromagnetic valve, a fourth electromagnetic valve, a fifth electromagnetic valve and a sixth electromagnetic valve, wherein a first interface of the third electromagnetic valve is communicated with a second interface of the flow chamber, a second interface of the third electromagnetic valve is communicated with a first interface of the buffer mechanism, a first interface of the fourth electromagnetic valve is communicated with the sheath fluid injector, a second interface of the fourth electromagnetic valve is communicated with a first interface of the first electromagnetic valve, a third interface of the fourth electromagnetic valve is respectively communicated with a first interface of the fifth electromagnetic valve and a first interface of the sixth electromagnetic valve, a second interface of the fifth electromagnetic valve is communicated with a third interface of the flow chamber, and a second interface of the sixth electromagnetic valve is communicated with a sample injector;
the sheath fluid injector is further configured to convey sheath fluid in the sheath fluid storage tank to a pipeline between the fourth solenoid valve and the first solenoid valve when the first interface of the sixth solenoid valve is not communicated with the second interface of the sixth solenoid valve, the first interface of the fifth solenoid valve is not communicated with the second interface of the fifth solenoid valve, the first interface of the third solenoid valve is not communicated with the second interface of the third solenoid valve, the first interface of the fourth solenoid valve is communicated with the second interface of the fourth solenoid valve, and the first interface of the first solenoid valve is communicated with the third interface of the first solenoid valve;
the sheath liquid injector is further used for conveying sheath liquid in a pipeline between the fourth electromagnetic valve and the first electromagnetic valve to the flow chamber when the first interface of the sixth electromagnetic valve is communicated with the second interface of the sixth electromagnetic valve, the first interface of the fifth electromagnetic valve is communicated with the second interface of the third electromagnetic valve, and the first interface of the fourth electromagnetic valve is communicated with the third interface of the fourth electromagnetic valve.
4. The fluid circuit system of claim 3, wherein the fluid circuit system further comprises: a sample injector, a measurement needle mechanism, and a seventh solenoid valve;
the first interface of the sample injector is communicated with the second interface of the sixth electromagnetic valve, the second interface of the sample injector is respectively communicated with the fourth interface of the flow chamber and the first interface of the seventh electromagnetic valve, and the second interface of the seventh electromagnetic valve is communicated with the first interface of the measuring needle mechanism;
the measuring needle mechanism is used for sucking a sample to be measured into the optical detection mechanism for detection;
the sheath fluid injector is used for conveying the sample to be tested to a pipeline between the seventh electromagnetic valve and the sample injector when the first interface of the seventh electromagnetic valve is communicated with the second interface of the seventh electromagnetic valve, the first interface of the sixth electromagnetic valve is communicated with the second interface of the sixth electromagnetic valve, and the first interface of the fourth electromagnetic valve is communicated with the third interface of the fourth electromagnetic valve;
the sheath liquid injector is used for conveying the sample to be tested in the pipeline between the seventh electromagnetic valve and the sample injector to the flow chamber when the first interface of the seventh electromagnetic valve is not communicated with the second interface of the seventh electromagnetic valve.
5. The fluid circuit system of any one of claims 3-4, further comprising: the device comprises a pumping pump, a waste liquid recovery tank and an eighth electromagnetic valve, wherein a first interface of the eighth electromagnetic valve is communicated with a second interface of the buffer mechanism, a second interface of the eighth electromagnetic valve is communicated with the pumping pump, and the pumping pump is communicated with the waste liquid recovery tank;
the pumping pump is used for discharging the liquid in the flow chamber to the waste liquid recovery tank when the first interface of the third electromagnetic valve is communicated with the second interface of the third electromagnetic valve, the first interface of the eighth electromagnetic valve is communicated with the second interface of the eighth electromagnetic valve, and the pumping pump is started.
6. The fluid circuit system of claim 5, further comprising: a ninth electromagnetic valve, wherein a first interface of the ninth electromagnetic valve is communicated with a second interface of the measuring needle mechanism, a second interface of the ninth electromagnetic valve is communicated with the pumping pump, and the pumping pump is communicated with the waste liquid recovery tank;
the sheath liquid injector is used for conveying sheath liquid in the sheath liquid injector to the measuring needle mechanism and discharging sheath liquid in the measuring needle mechanism to the waste liquid recovery tank when the first interface of the fourth electromagnetic valve is communicated with the third interface of the fourth electromagnetic valve, the first interface of the sixth electromagnetic valve is communicated with the second interface of the sixth electromagnetic valve, the first interface of the seventh electromagnetic valve is communicated with the second interface of the seventh electromagnetic valve, the first interface of the ninth electromagnetic valve is communicated with the second interface of the ninth electromagnetic valve and the pumping pump is started.
7. The fluid path system according to claim 6, wherein,
and the sheath liquid injector is used for conveying sheath liquid in the sheath liquid injector to the lower port of the swab of the measuring needle mechanism and discharging the sheath liquid through the upper port of the swab of the measuring needle mechanism when the first interface of the fourth electromagnetic valve is communicated with the second interface of the fourth electromagnetic valve, the first interface of the ninth electromagnetic valve is communicated with the second interface of the ninth electromagnetic valve and the pumping pump is started.
8. The fluid circuit system of claim 4 wherein the fluid circuit system comprises a plurality of fluid circuits,
the fluid path system further comprises: a tenth electromagnetic valve, wherein a first interface of the tenth electromagnetic valve is communicated with a third interface of the measuring needle mechanism, and a second interface of the tenth electromagnetic valve is respectively communicated with a first interface of the sixth electromagnetic valve and a third interface of the fourth electromagnetic valve;
the sheath fluid injector is used for conveying sheath fluid in the sheath fluid injector to the measuring needle mechanism when the first interface of the fourth electromagnetic valve is communicated with the third interface of the fourth electromagnetic valve, the first interface of the sixth electromagnetic valve is not communicated with the second interface of the sixth electromagnetic valve, and the first interface of the tenth electromagnetic valve is communicated with the second interface of the tenth electromagnetic valve.
9. An immunoassay according to any one of claims 1 to 8, wherein said immunoassay comprises a fluid pathway system.
10. A method of cleaning a liquid path system, wherein the method is based on a liquid path system according to any one of claims 1-8, the method comprising:
and conveying the cleaning solution in the cleaning solution storage tank to the flow chamber through the sheath solution injector so as to clean the flow chamber.
11. The method of claim 10, wherein the step of determining the position of the first electrode is performed,
the residence time of the cleaning liquid in the flow chamber is 3-15 minutes.
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