CN107345957A - A kind of liquid-way system of streaming immunoluminescence analyzer - Google Patents
A kind of liquid-way system of streaming immunoluminescence analyzer Download PDFInfo
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- CN107345957A CN107345957A CN201610293034.3A CN201610293034A CN107345957A CN 107345957 A CN107345957 A CN 107345957A CN 201610293034 A CN201610293034 A CN 201610293034A CN 107345957 A CN107345957 A CN 107345957A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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Abstract
The present invention relates to a kind of liquid-way system of streaming immunoluminescence analyzer, the liquid-way system of described streaming immunoluminescence analyzer includes：Sheath flow liquid bottle (1), peristaltic pump (2), valve Va (4), sample suction pump (6), triple valve (7), flow chamber (8), valve Vc (9), valve Vd (10), suction needle (11), waste liquid barrel (14) and necessary pipeline.The system also includes buffer (3) and purging system, and described purging system includes valve Vb (5), washer (12), liquid pump (13) and necessary pipeline.
Description
Technical field
The invention belongs to medical equipment technological field, in particular to a kind of liquid-way system of streaming immunoluminescence analyzer.
Background technology
Clinical biochemical tests and analyzes work has ELISA (ELISA), chemoluminescence method using general technology including immunoassay
(CLIA), chromatography (collaurum/fluorescence/above turn anti-), turbidimetry (transmission/scattering) etc..
The technology relative maturity of above-mentioned several determination methods, clinical practice are carried out than wide, still, with clinical molecular
The demand of detection/molecule diagnosis is constantly segmented, and is gradually shown the shortcomings that existing detection technique, specifically, existing ripe inspection
The shortcomings that survey technology, can be summarized as follows：
(1) ELISA (ELISA)：Single project can only be detected, testing procedure is cumbersome, time length (needing board-washing),
Poor repeatability (general CV values about 20%).
(2) chemoluminescence method (CLIA)：Single project can only be detected, testing procedure is cumbersome, time length (needing Magneto separate),
Cost is high.
(3) chromatography (collaurum/fluorescence/above turn anti-)：3 projects are at most detected simultaneously, and false positive, false negative are high, weight
Renaturation is poor (general CV values about 15%).
In view of the above-mentioned problems, streaming immunoluminescence analyzer is increasingly paid attention to by scientific research and clinical workers.
The content of the invention
The present invention provide it is a kind of can it is more convenient, efficiently sample is detected based on immunologic detection method (fluorescence/luminous)
Streaming immunoluminescence analyzer fluid path control system.
The fluid path control system includes：Sheath flow liquid bottle (1), triple valve (7), sample suction pump (6), suction needle (11), flowing
Room (8), valve c (9), peristaltic pump (2), valve a (4), valve d (10), waste liquid barrel (14) and necessary pipeline.
The fluid path rate-determining steps of described system are：
(1) whole valves are closed；Triple valve (7) makes sample suction pump (6) be connected with suction needle (11), and sample suction pump (6) is by sample
Originally and reagent mixed liquor is drawn onto the pipeline between triple valve (7) and sample suction pump (6)；
(2) triple valve (7) connection flow chamber (8) is controlled, opens valve c (9), sample suction pump (6) injects sample mixed liquor
To flow chamber (8)；
(3) triple valve (7) is connected to suction needle (11), and peristaltic pump (2) is opened, and opens valve a (4) and valve d (10), sheath
Sheath stream end of the flow liquid with sample mixed liquor by flow chamber (8), while detecting system collection signal analysis.
Further, the fluid path control system also includes：Buffer (3), the buffer (3) in peristaltic pump (2) and
Flow chamber adds between (8), for stablizing the speed of sheath stream.
Further, described fluid path control system also includes：Fluid path cleaning function module, described fluid path cleaning function module
Including：Valve b (5), liquid pump (13), washer (12) and necessary pipeline.
The described fluid path cleaning step comprising fluid path cleaning module fluid path control system is：
(1) shutoff valve a (4), open valve c (9), and triple valve connects flow chamber；
(2) valve b (5), while pump work of wriggling, the cleaning flow chamber of sheath flow liquid from bottom to top are opened；
(3) shutoff valve c (9), triple valve are connected to suction needle end；
(4) shutoff valve b (5), open valve a (4), open liquid pump (13), open peristaltic pump (2), suction needle (11) by
Step rises, and sheath flow liquid is to suction needle (11) outer wall washing；
(5) needle point is raised in washer (12), shutoff valve a (4), valve c (9), and triple valve (7) connects suction needle (11)
End；
(6) valve b (5) is opened, opens liquid pump (13), opens peristaltic pump (2), sheath flow liquid is clear to suction needle (11) inwall
Wash.
Brief description of the drawings
The design drawing of Fig. 1 streaming immunoluminescence analyzer fluid path operating schemes；
In diagram mark, 1：Sheath flow liquid bottle；2：Peristaltic pump；3：Buffer；4：Valve a；5：Valve b；6：Sample suction pump；7：
Triple valve；8：Flow chamber, 9：Valve c；10：Valve d；11：Suction needle；12：Washer；13：Liquid pump；14：Waste liquid
Bucket.
Embodiment
Fig. 1 show streaming immunoluminescence analyzer fluid path involved in the present invention, specifically, the system support sample and
Reagent hybrid detection, multiple reagents are supported to be used together.
Embodiment 1, fluid path control system operating scheme 1
When system starts, fluid path control the step of it is as follows：
(1) whole valves are closed；Triple valve (7) makes sample suction pump (6) be connected with suction needle (11), and sample suction pump (6) is by sample
Originally and reagent mixed liquor is drawn onto the pipeline between triple valve (7) and sample suction pump (6)；
(2) triple valve (7) connection flow chamber (8) is controlled, opens valve c (9), sample suction pump (6) injects sample mixed liquor
To flow chamber (8)；
(3) triple valve (7) is connected to suction needle (11), and peristaltic pump (2) is opened, and opens valve a (4), sheath flow liquid is with sample
This mixed liquor is by the sheath stream end of flow chamber (8), while detecting system collection signal analysis.
Embodiment 2, fluid path control system operating scheme 2
On the basis of the fluid path control system scheme described in embodiment 1, in order to obtain the sheath stream process of stabilized speed, wriggling
Buffer (3) is added between pump (2) and flow chamber (8).
Embodiment 3, fluid path control system operating scheme 3
On the basis of the fluid path control system scheme of embodiment 1 or 2, in order to reduce cross pollution, increase fluid path in systems
The control system scheme 3 of cleaning function module, the schematic diagram of control system scheme 3 are as shown in Figure 1.
The workflow of system schema 3 containing the cleaning function module is controlled, mixes and examined in the fluid path described in embodiment 1,2
Outside the flow of survey, in addition to extra fluid path cleaning function, the workflow of described fluid path cleaning are as follows：
(1) shutoff valve a (4), open valve c (9), and triple valve connects flow chamber；
(2) valve b (5), while pump work of wriggling, the cleaning flow chamber of sheath flow liquid from bottom to top are opened；
(3) shutoff valve c (9), triple valve are connected to suction needle end；
(4) shutoff valve b (5), open valve a (4), open liquid pump (13), open peristaltic pump (2), suction needle (11) by
Step rises, and sheath flow liquid is to suction needle (11) outer wall washing；
(5) needle point is raised in washer (12), shutoff valve a (4), valve c (9), and triple valve (7) connects suction needle (11)
End；
(6) valve b (5) is opened, opens liquid pump (13), opens peristaltic pump (2), sheath flow liquid is clear to suction needle (11) inwall
Wash.
Finally it should be noted that above example only supplies skilled artisan understands that the essence of the present invention, is not used as to this
The restriction of invention protection domain.
Claims (8)
- A kind of 1. liquid-way system of streaming immunoluminescence analyzer, it is characterised in that the liquid of described streaming immunoluminescence analyzer Road system includes：Sheath flow liquid bottle (1), peristaltic pump (2), valve Va (4), sample suction pump (6), triple valve (7), flow chamber (8), Valve Vc (9), valve Vd (10), suction needle (11), waste liquid barrel (14) and necessary pipeline.
- 2. the liquid-way system of the streaming immunoluminescence analyzer described in claim 1, it is characterised in that the fluid path of described system Rate-determining steps are：(1) whole valves are closed；Triple valve (7) makes sample suction pump (6) be connected with suction needle (11), and sample suction pump (6) is by sample Originally and reagent mixed liquor is drawn onto the pipeline between triple valve (7) and sample suction pump (6)；(2) triple valve (7) connection flow chamber (8) is controlled, opens valve Vc (9), sample suction pump (6) notes sample mixed liquor Enter to flow chamber (8)；(3) triple valve (7) is connected to suction needle (11), and peristaltic pump (2) is opened, and opens valve Va (4) and valve Vd (10), Sheath stream end of the sheath flow liquid with sample mixed liquor by flow chamber (8), while detecting system collection signal analysis.
- 3. the liquid-way system of streaming immunoluminescence analyzer according to claim 1 or 2, it is characterised in that the streaming The liquid-way system of immunoluminescence analyzer also includes：Buffer (3)；The buffer (3) is added in peristaltic pump (2) and stream Between dynamic room (8), for stablizing the speed of sheath stream.
- 4. the liquid-way system of streaming immunoluminescence analyzer according to claim 1 or 2, it is characterised in that described stream The liquid-way system of formula immunoluminescence analyzer also includes：Fluid path cleaning function module.
- 5. the liquid-way system of streaming immunoluminescence analyzer according to claim 4, it is characterised in that described fluid path is clear Washing functional module includes：Valve Vb (5), washer (12), liquid pump (13) and necessary pipeline.
- 6. the liquid-way system of streaming immunoluminescence analyzer according to claim 5, it is characterised in that described fluid path is clear Washing step is：(1) shutoff valve Va (4), open valve Vc (9), and triple valve connects flow chamber；(2) valve Vb (5), while pump work of wriggling, the cleaning flow chamber of sheath flow liquid from bottom to top are opened；(3) shutoff valve Vc (9), triple valve are connected to suction needle end；(4) shutoff valve Vb (5), valve Va (4) is opened, opens liquid pump (13), open peristaltic pump (2), suction needle (11) Progressively rise, sheath flow liquid is to suction needle (11) outer wall washing；(5) needle point is raised in washer (12), shutoff valve Va (4), valve Vc (9), and triple valve (7) connects suction needle (11) hold；(6) valve Vb (5) is opened, opens liquid pump (13), opens peristaltic pump (2), sheath flow liquid is clear to suction needle (11) inwall Wash.
- 7. the streaming immunoluminescence analyzer of the liquid-way system comprising any described streaming immunoluminescence analyzers of claim 1-6.
- 8. the liquid-way system of any described streaming immunoluminescence analyzers of claim 1-6 is preparing streaming immunoluminescence analyzer In application.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610293034.3A CN107345957A (en) | 2016-05-05 | 2016-05-05 | A kind of liquid-way system of streaming immunoluminescence analyzer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610293034.3A CN107345957A (en) | 2016-05-05 | 2016-05-05 | A kind of liquid-way system of streaming immunoluminescence analyzer |
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| CN107345957A true CN107345957A (en) | 2017-11-14 |
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| CN201610293034.3A Pending CN107345957A (en) | 2016-05-05 | 2016-05-05 | A kind of liquid-way system of streaming immunoluminescence analyzer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109444028A (en) * | 2018-12-26 | 2019-03-08 | 常州必达科生物科技有限公司 | A kind of particle analyzer and its liquid channel system, discharging of waste liquid method |
| CN109999924A (en) * | 2019-04-12 | 2019-07-12 | 长春工程学院 | A kind of multi-joint ion exchange experiments device |
| CN114113571A (en) * | 2020-08-27 | 2022-03-01 | 深圳市帝迈生物技术有限公司 | Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system |
-
2016
- 2016-05-05 CN CN201610293034.3A patent/CN107345957A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109444028A (en) * | 2018-12-26 | 2019-03-08 | 常州必达科生物科技有限公司 | A kind of particle analyzer and its liquid channel system, discharging of waste liquid method |
| CN109999924A (en) * | 2019-04-12 | 2019-07-12 | 长春工程学院 | A kind of multi-joint ion exchange experiments device |
| CN109999924B (en) * | 2019-04-12 | 2023-10-13 | 长春工程学院 | Multi-connected ion exchange experimental device |
| CN114113571A (en) * | 2020-08-27 | 2022-03-01 | 深圳市帝迈生物技术有限公司 | Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system |
| CN114113571B (en) * | 2020-08-27 | 2023-12-15 | 深圳市帝迈生物技术有限公司 | Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system |
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Application publication date: 20171114 |