CN113759137A - Sample detection device and sample detection method - Google Patents
Sample detection device and sample detection method Download PDFInfo
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- CN113759137A CN113759137A CN202010499353.6A CN202010499353A CN113759137A CN 113759137 A CN113759137 A CN 113759137A CN 202010499353 A CN202010499353 A CN 202010499353A CN 113759137 A CN113759137 A CN 113759137A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0437—Cleaning cuvettes or reaction vessels
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Abstract
The invention provides a sample detection device and a sample detection method, wherein the sample detection device comprises a pipeline, a sampling component, a detection component, a reaction component, a sheath fluid component and a cleaning component, wherein the sampling component, the detection component, the reaction component, the sheath fluid component and the cleaning component are sequentially connected with the pipeline; the sheath liquid assembly is connected to the detection assembly and used for providing sheath liquid for the detection assembly; the cleaning assembly comprises a first cleaning assembly, a second cleaning assembly and a third cleaning assembly; the reaction assembly is used for preparing a sample to be detected, the sampling assembly is used for pumping the sample to be detected into the pipeline, and the third cleaning assembly is used for pushing out the third cleaning solution and pushing the pumped part of the sample to be detected into the detection assembly through the third cleaning solution so as to be detected by the detection assembly. Wherein, the third cleaning assembly can wash the operation and push away the appearance operation, has saved dedicated sample subassembly that pushes away, has simplified liquid way structure and instrument inner space and has taken up to the third cleaning assembly who is used for pushing away the appearance is connected on the pipeline in reaction assembly and determine module outside, has simplified the pipeline, has reduced the pollution of the sample that awaits measuring.
Description
Technical Field
The invention relates to the technical field of medical detection and analysis, in particular to a sample detection device and a sample detection method.
Background
The existing sheath flow detection liquid path system comprises a pipeline, a sampling assembly, a detection assembly, a sample pushing assembly and a reaction assembly, wherein the sampling assembly, the detection assembly, the sample pushing assembly and the reaction assembly are sequentially connected with the pipeline.
It should be noted that: the sample pushing assembly is connected between the reaction assembly and the detection assembly, a Y-shaped liquid path formed by the three branch pipes through the three-way joint is formed on the liquid path, and the inventor researches and discovers that after the instrument is used for a period of time, the sample pushing assembly can become a pollution source influencing a sample to be detected at the position of the Y-shaped liquid path formed between the reaction assembly and the detection assembly, the sample to be detected prepared in the reaction assembly is pumped to a pipeline through the sampling assembly, the sample to be detected can be polluted or diluted when flowing through the position (Y-shaped liquid path), the polluted sample to be detected enters the detection device again to be detected, and the detection accuracy can be obviously influenced.
Disclosure of Invention
The invention provides a sample detection device and a sample detection method, which aim to solve the technical problem that a sample can be diluted or polluted before entering a detection assembly in the prior art.
In order to solve the technical problems, the invention adopts a technical scheme that: there is provided a sample detection device comprising:
a pipeline;
the sampling component, the detection component and the reaction component are sequentially connected with the pipeline;
the sheath liquid assembly is connected to the detection assembly and used for providing sheath liquid for the detection assembly;
the cleaning assembly comprises a first cleaning assembly, a second cleaning assembly and a third cleaning assembly, the first cleaning assembly is connected to the reaction assembly and used for providing a first cleaning solution for the reaction assembly to clean, the second cleaning assembly and the third cleaning assembly are connected with the pipeline and located on one side, away from the detection assembly, of the reaction assembly and used for providing a second cleaning solution and a third cleaning solution for the pipeline and the reaction assembly to clean, and the cleaning performance of the first cleaning solution and the third cleaning solution is weaker than that of the second cleaning solution;
the reaction assembly is used for preparing a sample to be detected, the sampling assembly is used for extracting the sample to be detected to the pipeline, and the third cleaning assembly is used for pushing out the third cleaning solution and pushing the extracted part of the sample to be detected into the detection assembly through the third cleaning solution so as to detect the sample by the detection assembly.
In order to solve the technical problem, the invention adopts another technical scheme that: the method for detecting the sample based on the sample detection device comprises the following steps: preparing a sample to be tested in the reaction assembly;
pumping the sample to be tested into the pipeline by using the sampling component;
actuating the sheath fluid assembly to form a sheath flow;
and pushing out a third cleaning solution by using the third cleaning component, and pushing the extracted part of the sample to be detected into the detection component by using the third cleaning solution so as to detect by using the detection component.
In order to solve the technical problem, the invention adopts another technical scheme that: the method for detecting the sample based on the sample detection device comprises the following steps:
preparing a first sample to be detected in the first reaction tank when the sixth selective valve and the seventh selective valve are in a closed state;
opening the sixth gate valve to draw the first sample to be measured into the pipeline using the sampling assembly and to be held between the second gate valve and the first reaction cell;
opening the fourth or fifth gate valve to activate the sheath fluid assembly to form a sheath flow;
opening the eleventh or twelfth selective valve to push out a third cleaning solution by using the third cleaning component and enter the first reaction tank through the sixth selective valve to clean the reaction tank;
and closing the sixth selective valve to push the extracted part of the first sample to be detected into the detection component through the third cleaning solution so as to be detected by the detection component.
The invention has the beneficial effects that: different from the prior art, in the sample detection device and the sample detection method provided by the invention, the third cleaning component can perform cleaning operation and sample pushing operation, saves special sample pushing components, simplifies the liquid path structure and the occupation of the internal space of the instrument, and the third cleaning component for pushing the sample is connected on the pipeline outside the reaction component and the detection component, thus simplifying the pipeline connection between the preparation and the detection of the sample to be detected, reducing the pollution of the sample to be detected, by setting the power source of the third cleaning assembly and/or the power source of the sheath fluid assembly as a positive pressure source, the device can provide stable pressure to further eliminate sample flow fluctuation caused by pushing liquid by an injector, and different liquid flow speeds can be controlled by controlling the pressure of a positive pressure source or arranging a throttle valve on a branch pipe or replacing branch pipes with different pipe diameters so as to adapt to the detection requirements of different projects.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the invention, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:
FIG. 1 is a simplified block diagram of a first embodiment of a sample testing device according to the present invention;
FIG. 2 is a simplified fluid path schematic of a first embodiment of a sample testing device according to the present invention;
FIG. 3 is a simplified block diagram of a second embodiment of a sample testing device according to the present invention;
FIG. 4 is a simplified fluid path diagram of a second embodiment of a sample testing device according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive work based on the embodiments of the present invention, are within the scope of the present invention.
It should be noted that, if directional indications (such as up, down, left, right, front, and back … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative positional relationship between the components, the movement situation, and the like in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indications are changed accordingly.
In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
Referring to fig. 1, a sample detecting device according to an embodiment of the present invention includes a pipeline 60, a sampling module 10, a detecting module 20, a reaction module 30, a sheath liquid module 40, and a cleaning module, which are sequentially connected to the pipeline 60. The reaction unit 30 may include a first reaction tank 31 and a second reaction tank 32, and the washing unit may include a first washing unit 51, a second washing unit 52, and a third washing unit 53.
The sheath fluid assembly 40 is coupled to the sensing assembly 20 for providing sheath fluid to the sensing assembly 20.
The first cleaning assembly 51 is connected to the first reaction tank 31 and the second reaction tank 32 and is used for providing a first cleaning solution for the first reaction tank 31 and the second reaction tank 32 for cleaning, the second cleaning assembly 52 is used for providing a second cleaning solution for the pipeline 60, the first reaction tank 31 and the second reaction tank 32 for cleaning, and the third cleaning assembly 53 is used for providing a third cleaning solution for the pipeline 60, the first reaction tank 31 and the second reaction tank 32 for cleaning, wherein the cleaning performance of the first cleaning solution and the third cleaning solution is weaker than that of the second cleaning solution.
The first reaction tank 31 and the second reaction tank 32 are used for preparing a sample to be detected, the sampling assembly 10 is used for pumping the sample to be detected into the pipeline 60, and the third cleaning assembly 53 is used for pushing out a third cleaning solution to perform a cleaning operation and pushing a part of the pumped sample to be detected into the detection assembly 20 through the third cleaning solution so as to be detected by the detection assembly 20.
In an embodiment of the present invention, the first cleaning solution may be a diluent or a cleaning solution, and the second cleaning solution may be a hemolytic agent or a cleaning solution, for example, the second cleaning solution may be a RET (reticulocyte) diluent or WNR hemolytic agent, preferably WNR hemolytic agent, because the cleaning effect of the RET diluent is relatively weak, and the cleaning effect of the WNR hemolytic agent is relatively strong; or the second cleaning solution may be a cleaning solution different from the diluent or the hemolytic agent, for example, a cleaning solution containing sodium hypochlorite and a surfactant as main components, a cleaning solution containing an enzyme as a main component, or the like. And the cleaning effect of the second cleaning solution is obviously better than that of the first cleaning solution. The third wash solution may be a dilution solution. Since the second washing solution is used and the line 60 may have some hemolytic agent or washing agent remaining therein, which may affect the sample to be tested next, for example, the strong washing solution may affect the change of cell morphology, the line 60 washed by the second washing solution needs to be washed again, and therefore, the second washing solution is used and then the line is washed by the third washing solution in principle.
In addition, because the first reaction tank 31 and the second reaction tank 32 are large in size, if the second cleaning assembly 52 or the third cleaning assembly 53 is adopted to clean the first reaction tank 31 and the second reaction tank 32, and because the liquid contained in the second cleaning assembly 52 and the third cleaning assembly 53 is small in size and relatively far in path, the reaction tank is cleaned in a long time, so that the first cleaning assembly 51 is added, the first cleaning assembly 51 is directly connected to the first reaction tank 31 and the second reaction tank 32 in a short distance, the first cleaning liquid can be quickly provided, the cleaning time and the cleaning path can be shortened, and the cleaning and detection efficiency can be improved.
The reaction assemblies 30 may be a plurality of sets (not limited to the first reaction cell 31 and the second reaction cell 32 in the figure), and the plurality of sets of reaction assemblies 30 are used for preparing at least two different samples to be tested, such as RET samples and WDF samples.
The power source of the third cleaning assembly 53 and/or the power source of the sheath fluid assembly 40 may be a positive pressure source, which may provide a stable pressure, which may eliminate sample flow fluctuation caused when an injector is used to push fluid, and improve detection accuracy.
The third cleaning assembly 53 can be used to provide a plurality of different speeds of the third cleaning fluid and the sheath fluid assembly 40 can be used to provide a plurality of different speeds of the sheath fluid.
Specifically, different speeds can be provided by adjusting the pressure of the positive pressure source, or different speeds can be provided by controlling the resistance of the branch pipes, wherein the resistance of the branch pipes can be controlled by arranging the throttle valves 41 on the corresponding branch pipes, or by adopting the branch pipes with different pipe diameters for liquid path communication, and the cost of the latter mode is relatively lower.
Referring to fig. 3 and 4, the sample testing device may further include a sample pushing assembly 70 (e.g., a syringe 70), the third cleaning assembly 53 is connected to the pipeline 60 through a liquid pushing cavity of the syringe 70, the syringe 70 can function as an auxiliary liquid pushing device, and the syringe 70 can provide a faster acceleration, which can rapidly push the liquid in the pipeline 60 to flow when needed, thereby shortening the testing time to a certain extent.
As shown in fig. 2, the sample testing apparatus includes a pipeline 60, a sampling module 10, a testing module 20, a first reaction tank 31, a second reaction tank 32, a sheath fluid module 40, a first cleaning module 51, a second cleaning module 52, and a third cleaning module 53.
The sampling component 10, the detection component 20, the first reaction tank 31 and the second reaction tank 32 are sequentially connected with a pipeline 60. The sheath liquid assembly 40 is connected to the detection assembly 20 and is used for providing sheath liquid for the detection assembly 20, the first cleaning assembly 51 is connected to the first reaction tank 31 and the second reaction tank 32 and is used for providing first cleaning liquid for the first reaction tank 31 and the second reaction tank 32 to clean, the second cleaning assembly 52 is used for providing second cleaning liquid for the pipeline 60, the first reaction tank 31 and the second reaction tank 32 to clean, the third cleaning assembly 53 is used for providing third cleaning liquid for the pipeline 60, the first reaction tank 31 and the second reaction tank 32 to clean, and the cleaning performance of the first cleaning liquid and the third cleaning liquid is weaker than that of the second cleaning liquid.
The sampling assembly 10 can be respectively connected with positive pressure and negative pressure through a first selective valve 1 and respectively connected with a waste liquid pool and a pipeline 60 through a second selective valve 2; the sampling assembly 10 may be a fixed displacement pump or a syringe, and the sampling assembly 10 may perform a liquid pumping operation or a waste discharging operation by switching the second gate valve 2.
The output end of the detection component 20 is connected with the waste liquid pool through a branch pipe provided with the third gate valve 3, and the detection component 20 can adopt sheath flow impedance method or fluorescence detection method commonly used in the field for detection. The detection assembly 20 has an inner flow channel and an outer flow channel which are nested, wherein the inner flow channel is communicated with the pipeline 60 to receive a sample to be detected input in the pipeline 60 to pass through, the outer flow channel is communicated with the sheath liquid assembly 40 to receive a sheath liquid to pass through, when the two flow channels converge, the sheath liquid carries the sample to be detected to flow to form a sample flow, and the sample flow passes through a sheath flow detection area to perform classified statistics and/or counting statistics, or perform qualitative and/or quantitative statistics. .
The sheath liquid component 40 is connected to the outer flow channel of the detection component 20 through branch pipes which are connected in parallel and are respectively provided with a fourth gate valve 4 and a fifth gate valve 5, and at least one branch pipe is provided with a throttle valve 41.
The output end of the first reaction tank 31 is connected with the pipeline 60 through the branch pipe provided with the sixth gate valve 6, the waste discharge end of the first reaction tank 31 is connected with the waste liquid tank through the branch pipe provided with the seventh gate valve 7, the output end of the second reaction tank 32 is connected with the pipeline 60 through the branch pipe provided with the ninth gate valve 9, and the waste discharge end of the second reaction tank 32 is connected with the waste liquid tank through the branch pipe provided with the tenth gate valve 10'.
The first cleaning assembly 51 is connected to the first reaction tank 31 and the second reaction tank 32 through branch pipes provided with the eighth gate valve 8 to supply the first cleaning solution to the first reaction tank 31 and the second reaction tank 32 when necessary.
The second cleaning assembly 52 is connected to the line 60 via a branch provided with a thirteenth gate valve 13.
The third cleaning assembly 53 is connected to the pipeline 60 through branch pipes which are connected in parallel and respectively provided with the eleventh selective valve 11 and the twelfth selective valve 12, and at least one branch pipe is provided with a throttle valve 41.
The invention also provides a method for detecting a sample based on the sample detection device, which comprises the following steps:
cleaning the reaction assembly 30 with a first cleaning solution; and/or
Cleaning line 60 using sampling assembly 10 in conjunction with second cleaning assembly 52; and/or
The sampling assembly 10 and the third cleaning assembly 53 are used for matching with a cleaning pipeline 60, wherein the cleaning step is a pretreatment step for preparing a sample to be detected, and is selectively used in an actual situation without specific limitation;
preparing a sample to be tested in the reaction assembly 30;
using the sampling assembly 10 to draw a sample to be tested into the pipeline 60;
activating the sheath fluid assembly 40 to form a sheath flow;
in the step, the third cleaning solution flows in the pipeline 60 at a first speed to clean part of the pipeline 60, and flows in the pipeline 60 at a second speed after a preset time to perform sample pushing work, wherein the second speed is less than or equal to the first speed;
the reaction unit 30 is cleaned by the first cleaning unit 51 to prepare the next sample to be tested.
When the reaction component 30 includes the first reaction cuvette 31 and the second reaction cuvette 32, the aforesaid sample to be tested is the first sample to be tested prepared in the first reaction cuvette 31, and accordingly, the step of pushing out the third cleaning solution by using the third cleaning component 53 and pushing the extracted part of the first sample to be tested into the detecting component 20 by the third cleaning solution for the detecting component 20 to perform the testing further includes the following steps:
cleaning line 60 using sampling assembly 10 in conjunction with second cleaning assembly 52;
by using the sampling assembly 10 and the third cleaning assembly 53 to cooperate with the cleaning pipeline 60, the second cleaning assembly 52 and the third cleaning assembly 53 can be used sequentially each time, or the second cleaning assembly 52 can be used at regular intervals or regular times;
preparing a second sample to be tested in the second reaction cell 32;
pumping a second sample to be tested into the channel 60 using the sampling assembly 10;
activating the sheath fluid assembly 40 to form a sheath flow;
in the step, the third cleaning solution flows in the pipeline 60 at a first speed to clean part of the pipeline 60, and flows in the pipeline 60 at a second speed after a preset time to perform sample pushing operation, the second speed is less than or equal to the first speed, the speed of the third cleaning component 53 during cleaning operation and sample pushing operation can be controlled differently, the detection time can be shortened to a certain extent, and the detection efficiency is further improved.
It is understood that the use of the second cleaning assembly 52 can be selectively used according to different detection items; or selectively applied according to the number of samples tested, or according to the time of use of the instrument. The use of the second cleaning solution and the third cleaning solution can adopt different use logic sequences according to actual conditions.
With continued reference to fig. 2 and 4, the present invention further provides a method for performing a sample test, comprising the following steps:
opening the eighth gate valve 8 to clean the first reaction tank 31 through the first cleaning assembly 51; and/or
Opening thirteenth gate valve 13 to purge line 60 using second purge assembly 52 in conjunction with sampling assembly 10; and/or
Opening the eleventh selective valve 11 or the twelfth selective valve 12 to enable the third cleaning component 53 to cooperate with the sampling component 10 to clean the pipeline 60, wherein the above cleaning step is a pretreatment step for preparing a sample to be detected, and is selectively used in practical situations without specific limitation, for example, to improve the detection speed and reasonably perform resource scheduling, the pipeline 60 may be selectively cleaned by using the second cleaning component 52 or the third cleaning component 53 while the first cleaning component 51 is used for cleaning, or the pipeline 60 may be cleaned by using the third cleaning component 53 after the second cleaning component 52 is used for cleaning the pipeline 60;
when the sixth gate valve 6 and the seventh gate valve 7 are in the closed state, the first sample to be tested is prepared in the first reaction tank 31, and also when the first sample to be tested is prepared, because the preparation time is long, the second cleaning component 52 or the third cleaning component 53 can be used for cleaning the pipeline 60, or the second cleaning component 52 is used for cleaning the pipeline 60 and then the third cleaning component 53 is used for cleaning the pipeline 60;
opening the sixth gate valve 6 to draw the first sample to be measured into the line 60 using the sampling assembly 10 and to keep it between the second gate valve 2 and the first reaction tank 31;
opening the fourth gate valve 4 or the fifth gate valve 5 to activate the sheath fluid assembly 40 to form a sheath flow;
opening the eleventh selective valve 11 or the twelfth selective valve 12 to push out the third cleaning solution by using the third cleaning component 53 and enter the first reaction tank 31 through the sixth selective valve 6 for cleaning the reaction tank;
closing the sixth selective valve 6 to push the extracted part of the first sample to be detected into the detecting assembly 20 through the third cleaning liquid for detection by the detecting assembly 20;
opening the eighth gate valve 8 to clean the first reaction tank 31 through the first cleaning assembly 51;
opening thirteenth gate valve 13 to purge line 60 using second purge assembly 52 in conjunction with sampling assembly 10;
opening the eleventh selective valve 11 or the twelfth selective valve 12 to enable the third cleaning assembly 53 to cooperate with the sampling assembly 10 to clean the pipeline 60, wherein the second cleaning assembly 52 and the third cleaning assembly 53 can be sequentially used each time, or the second cleaning assembly 52 can be used at regular intervals or regular times, and it can be understood that the second cleaning assembly 52 can be selectively used according to different detection items; or selectively applied according to the number of samples tested, or according to the time of use of the instrument. The use of the second cleaning solution and the third cleaning solution can adopt different use logic sequences according to actual conditions;
preparing a second sample to be detected in the second reaction tank 32 when the ninth gate valve 9 and the tenth gate valve 10' are in the closed state;
opening the ninth gate valve 9 to draw the second sample to be tested into the pipeline 60 by using the sampling assembly 10 and keeping the second sample to be tested between the second gate valve 2 and the second reaction tank 32;
opening the fourth gate valve 4 or the fifth gate valve 5 to activate the sheath fluid assembly 40 to form a sheath flow;
opening the eleventh selective valve 11 or the twelfth selective valve 12 to push out the third cleaning solution by using the third cleaning component 53 and enter the second reaction tank 32 through the ninth selective valve 9 for cleaning the reaction tank;
and closing the ninth gate valve 9 to push the extracted part of the second sample to be detected into the detection assembly 20 through the third cleaning liquid so as to be detected by the detection assembly 20.
It is understood that there is no specific sequence between the above steps, and other steps can be performed simultaneously when performing some steps, for example, when pushing the sample to the flow chamber (detection component 20) for detection, the first cleaning solution can be used to clean the reaction chamber at the same time; and/or when the sampling assembly 10 samples the sample to be detected in the first reaction tank 31 to the pipeline 60, the first cleaning liquid can be used for cleaning the second reaction tank 32 at the same time; and/or the first reaction cell 31 and/or the second reaction cell 32 can simultaneously clean the pipeline 60 and/or the flow chamber (the detection assembly 20) when preparing the sample liquid to be detected; and/or the sample to be tested in the first reaction cell 31 is subjected to sample preparation and/or detection, and simultaneously, the pipeline or the device through which the sample to be tested in the second reaction cell 32 may flow is cleaned.
In summary, it is easily understood by those skilled in the art that in the sample detection apparatus and the sample detection method provided by the present invention, the third cleaning component 53 can perform cleaning operation and sample pushing operation, thereby saving dedicated sample pushing components, simplifying the liquid path structure and the occupation of the internal space of the apparatus, and the third cleaning component 53 for sample pushing is connected to the pipeline outside the reaction component 30 and the detection component 20, simplifying the pipeline connection between the preparation and the detection of the sample to be detected, reducing the contamination of the sample to be detected, providing stable pressure by setting the power source of the third cleaning component 53 and/or the power source of the sheath liquid component as a positive pressure source, thereby eliminating the sample flow fluctuation caused by the liquid pushing with the syringe, controlling the pressure of the positive pressure source or providing the throttle valve 41 on the branch pipe or replacing the branch pipes with different pipe diameters to control different liquid flow velocities to adapt to the detection requirements of different items, for example, the third wash assembly 53 at a higher speed and the sheath fluid assembly 40 at a higher speed may form a fast and wide sample stream within the detection assembly 20 for detecting leukocytes; the third wash assembly 53 at a lower speed and the sheath fluid assembly 40 at a lower speed may create a slow and narrow sample flow within the detection assembly 20 for detecting platelets; the sample streams of particular needs may also be formed by different combinations.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (18)
1. A sample testing device, comprising:
a pipeline;
the sampling component, the detection component and the reaction component are sequentially connected with the pipeline;
the sheath liquid assembly is connected to the detection assembly and used for providing sheath liquid for the detection assembly;
the cleaning assembly comprises a first cleaning assembly, a second cleaning assembly and a third cleaning assembly, the first cleaning assembly is connected to the reaction assembly and used for providing a first cleaning solution for the reaction assembly to clean, the second cleaning assembly and the third cleaning assembly are connected with the pipeline and located on one side, away from the detection assembly, of the reaction assembly and used for providing a second cleaning solution and a third cleaning solution for the pipeline and the reaction assembly to clean, and the cleaning performance of the first cleaning solution and the third cleaning solution is weaker than that of the second cleaning solution;
the reaction assembly is used for preparing a sample to be detected, the sampling assembly is used for extracting the sample to be detected to the pipeline, and the third cleaning assembly is used for pushing out the third cleaning solution and pushing the extracted part of the sample to be detected into the detection assembly through the third cleaning solution so as to detect the sample by the detection assembly.
2. The sample testing device according to claim 1, wherein:
the reaction components are in multiple groups, and the multiple groups of reaction components are used for preparing at least two different samples to be tested.
3. The sample testing device according to claim 1, wherein:
the cleaning assembly comprises a first cleaning assembly, a second cleaning assembly and a third cleaning assembly, the first cleaning assembly is connected to the reaction assembly and used for providing first cleaning liquid for cleaning, the second cleaning assembly is connected with the third cleaning assembly and located on the pipeline, the reaction assembly is far away from one side of the detection assembly and used for providing second cleaning liquid and third cleaning liquid for cleaning, and the reaction assembly is connected with the pipeline.
4. The sample testing device according to claim 1, wherein:
and the power source of the third cleaning assembly and/or the power source of the sheath liquid assembly is a positive pressure source.
5. The sample testing device according to claim 4, wherein:
and providing a third cleaning solution with different speeds by adjusting the pressure of the positive pressure source.
6. The sample testing device according to claim 1, wherein:
the sheath fluid with different speeds is provided by controlling the resistance of the branch pipe.
7. The sample testing device according to claim 6, wherein:
the sheath liquid assembly is connected to the detection assembly through branch pipes connected in parallel, a throttle valve is arranged on at least one branch pipe, or the pipe diameters of the branch pipes connected in parallel are different; and/or
The third cleaning component is connected with the pipeline through branch pipes which are connected in parallel, and a throttle valve is arranged on at least one branch pipe, or the pipe diameters of the branch pipes which are connected in parallel are different.
8. The sample testing device according to claim 1, wherein:
the sample detection device also comprises an injector, and the third cleaning component is connected with the pipeline through a liquid pushing cavity of the injector.
9. The sample testing device according to claim 1, wherein:
the sampling assembly is respectively connected with the waste liquid pool and the pipeline through a second gating valve;
the output end of the detection assembly is connected with the waste liquid pool through a branch pipe provided with a third gating valve;
the sheath liquid assembly is connected to the detection assembly through branch pipes which are connected in parallel and respectively provided with a fourth selective valve and a fifth selective valve;
the reaction assembly comprises a first reaction tank and a second reaction tank, the output end of the first reaction tank is connected with the pipeline through a branch pipe provided with a sixth selective valve, the waste discharge end of the first reaction tank is connected with the waste liquid tank through a branch pipe provided with a seventh selective valve, the output end of the second reaction tank is connected with the pipeline through a branch pipe provided with a ninth selective valve, and the waste discharge end of the second reaction tank is connected with the waste liquid tank through a branch pipe provided with a tenth selective valve;
the first cleaning assembly is connected to the first reaction tank and the second reaction tank through branch pipes provided with eighth gating valves;
the second cleaning assembly is connected with the pipeline through a branch pipe provided with a thirteenth gating valve;
the third cleaning component is connected with the pipeline through branch pipes which are connected in parallel and are respectively provided with an eleventh selective valve and a twelfth selective valve.
10. A method for performing sample testing based on the sample testing device according to any one of claims 1 to 9, comprising:
preparing a sample to be tested in the reaction assembly;
pumping the sample to be tested into the pipeline by using the sampling component;
actuating the sheath fluid assembly to form a sheath flow;
and pushing out a third cleaning solution by using the third cleaning component, and pushing the extracted part of the sample to be detected into the detection component by using the third cleaning solution so as to detect by using the detection component.
11. The method of claim 10, wherein prior to preparing a sample to be tested within the reaction assembly, further comprising:
cleaning the reaction assembly by using a first cleaning assembly; and/or
Cleaning the pipeline by using the sampling assembly and the second cleaning assembly in a matching manner; and/or
Cleaning the tubing using the sampling assembly in cooperation with the third cleaning assembly.
12. The method of claim 10, wherein the step of pushing out a third washing solution by using the third washing assembly and pushing the extracted sample to be tested into the testing assembly by the third washing solution for testing by the testing assembly further comprises:
the third cleaning liquid flows in the pipeline at a first speed to perform partial pipeline cleaning, and flows in the pipeline at a second speed after a preset time to perform sample pushing work, wherein the second speed is less than or equal to the first speed.
13. The method of claim 10, wherein the step of pushing out a third washing solution by using the third washing assembly and pushing the extracted sample to be tested into the testing assembly by the third washing solution for testing by the testing assembly further comprises:
and cleaning the reaction assembly by using the first cleaning assembly.
14. The method of claim 10, wherein the reaction assembly comprises a first reaction cuvette and a second reaction cuvette, the sample to be tested is a first sample to be tested prepared in the first reaction cuvette, and the step of pushing out a third cleaning solution by using the third cleaning assembly and pushing the extracted portion of the first sample to be tested into the detection assembly by the third cleaning solution for detection by the detection assembly further comprises:
cleaning the tubing using the sampling assembly in cooperation with the third cleaning assembly;
preparing a second sample to be detected in the second reaction tank;
drawing the second sample to be tested into the pipeline by using the sampling component;
actuating the sheath fluid assembly to form a sheath flow;
and pushing out a third cleaning solution by using the third cleaning component, and pushing the extracted part of the second sample to be detected into the detection component by using the third cleaning solution so as to detect by the detection component.
15. A method for performing sample detection based on the sample detection device according to claim 9, comprising:
preparing a first sample to be detected in the first reaction tank when the sixth selective valve and the seventh selective valve are in a closed state;
opening the sixth gate valve to draw the first sample to be measured into the pipeline using the sampling assembly and to be held between the second gate valve and the first reaction cell;
opening the fourth or fifth gate valve to activate the sheath fluid assembly to form a sheath flow;
opening the eleventh or twelfth selective valve to push out a third cleaning solution by using the third cleaning component and enter the first reaction tank through the sixth selective valve to clean the reaction tank;
and closing the sixth selective valve to push the extracted part of the first sample to be detected into the detection component through the third cleaning solution so as to be detected by the detection component.
16. The method of claim 15, further comprising, before preparing the first sample to be measured in the first reaction cell while the sixth gate valve and the seventh gate valve are in the closed state:
opening the eighth gate valve to clean the first reaction tank through the first cleaning assembly; and/or
Opening the thirteenth gate valve to purge the pipeline using the second purge component in cooperation with the sampling component; and/or
And opening the eleventh selective valve or the twelfth selective valve to enable the third cleaning component to cooperate with the sampling component to clean the pipeline.
17. The method of claim 15, wherein the step of closing the sixth gate valve to push the withdrawn portion of the first sample to be tested into the testing assembly through the third cleaning solution for testing by the testing assembly further comprises:
and opening the eighth selective valve to clean the first reaction tank through the first cleaning assembly.
18. The method of claim 15, wherein the step of closing the sixth gate valve to push the withdrawn portion of the first sample to be tested into the testing assembly through the third washing liquid for testing by the testing assembly further comprises:
opening the eleventh or twelfth option valve to enable the third cleaning component to cooperate with the sampling component to clean the pipeline;
preparing a second sample to be detected in the second reaction tank when the ninth selective valve and the tenth selective valve are in a closed state;
opening the ninth gate valve to draw the second sample to be tested into the pipeline by using the sampling component;
opening the fourth or fifth gate valve to activate the sheath fluid assembly to form a sheath flow;
opening the eleventh or twelfth selective valve to push out a third cleaning solution by using the third cleaning component and enter the second reaction tank through the ninth selective valve to clean the reaction tank;
and closing the ninth selective valve to push the extracted part of the second sample to be detected into the detection assembly through the third cleaning solution so as to be detected by the detection assembly.
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