CN116337729A

CN116337729A - Blood cell analysis equipment

Info

Publication number: CN116337729A
Application number: CN202310634706.2A
Authority: CN
Inventors: 刘俊龙; 褚聪
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2023-05-31
Filing date: 2023-05-31
Publication date: 2023-06-27

Abstract

The application discloses blood cell analysis equipment, including flow cell, main line, at least two sample reaction unit, sheath liquid supply unit and propellant liquid supply unit. The sample reaction unit is used for conveying unused sample liquid to the main pipeline, the sheath liquid supply unit supplies sheath liquid to the sheath liquid receiving part of the flow chamber in at least two sheath liquid thrust steps, and the propellant liquid supply unit supplies propellant liquid to the main pipeline in at least two propellant liquid thrust steps so as to push the sample liquid in the main pipeline to the sample receiving part of the flow chamber through the propellant liquid. At least two sheath liquid thrust gears of sheath liquid supply unit and at least two propellant liquid thrust gears of propellant liquid supply unit mutually support to realize advancing sheath liquid and propellant liquid with different propulsion pressure, satisfy multiple detection channel to the propulsion requirement of sheath liquid and propellant liquid, improve the practicality and the detection accuracy of blood cell analysis equipment, improve the user and experience the use of this application's blood cell analysis equipment.

Description

Blood cell analysis equipment

Technical Field

The present application relates to the field of sample analysis technology, and in particular, to a blood cell analysis apparatus.

Background

In the prior art, the flow laser scattering combined fluorescent staining technology has great advantages for analyzing blood cells, both in terms of parameters and in terms of evaluation. When measuring blood cells, the flow laser scattering technology needs to make cells in a blood cell suspension line by line through an optical flow chamber under the wrapping of a reagent in a sheath flow, and irradiates the optical flow chamber with laser, so as to irradiate the cells line by line, obtain front scattered light, side scattered light and side fluorescence of the laser irradiated cells, and count and classify the cells.

Taking the example of white blood cell detection in blood cell detection, the flow laser scattering technology is required to detect WNR, WDF, WPC detection channels, the example of red blood cell and platelet detection is required to detect RET and RLT-F detection channels by using the flow laser scattering technology, and different detection channels have corresponding detection requirement detection design requirements, however, the existing blood cell analyzer has the defect that the detection design requirements of different detection channels cannot be met, and has the problem of low accuracy.

Disclosure of Invention

The application provides a blood cell analysis device to solve the above technical problems.

The blood cell analysis apparatus includes:

a flow chamber including a sample receiving member for receiving a sample liquid and a sheath liquid receiving member for receiving a sheath liquid by which the sample liquid output from the sample receiving member is wrapped, a sheath liquid sample laminar flow being formed in the flow chamber;

a main line, the sample receiving member being connected to the main line;

the at least two sample reaction units are respectively connected with the main pipeline, are used for respectively preparing different types of sample liquids, are respectively and selectively communicated with the main pipeline, and are used for conveying the different types of sample liquids to the main pipeline in a time-sharing manner;

a sheath liquid supply unit configured to supply the sheath liquid to the sheath liquid receiving member in at least two sheath liquid thrust steps;

a propellant liquid supply unit for supplying the propellant liquid to the main line in at least two propellant liquid thrust steps to push the sample liquid in the main line into the sample receiving member by the propellant liquid.

Wherein the blood cell analysis apparatus further comprises a controller for:

firstly controlling the propulsion liquid supply unit to push the propulsion liquid in the at least two propulsion liquid thrust gears with the propulsion pressure of a first propulsion liquid thrust gear;

Controlling the propulsion liquid supply unit to push the propulsion liquid in the at least two propulsion liquid thrust gears with the propulsion pressure of the second propulsion liquid thrust gear so as to push the sample liquid from the main pipeline into the sample receiving piece; the propulsion pressure of the first propulsion liquid thrust gear is larger than that of the second propulsion liquid thrust gear.

The propulsion liquid supply unit comprises a first propulsion liquid pipeline and a second propulsion liquid pipeline, and the first propulsion liquid pipeline and the second propulsion liquid pipeline are respectively connected with the main pipeline;

the controller is used for:

firstly controlling at least the first propellant line to push the propellant at the propulsion pressure of the first propellant thrust gear; then controlling the second propellant liquid pipeline to push the propellant liquid by the propulsion pressure of the second propellant liquid propulsion gear;

and/or, the controller is further configured to:

and in the process of cleaning the sample reaction unit, controlling at least through the first propellant liquid pipeline, and pushing the propellant liquid by the propulsion pressure of the third propellant liquid propulsion gear in the at least two propellant liquid propulsion gears so as to clean the sample reaction unit.

Wherein the blood cell analysis apparatus further comprises a controller for:

controlling the sheath fluid supply unit to supply the sheath fluid to the sheath fluid receiving member at the propulsion pressure of the first sheath fluid thrust gear among the at least two sheath fluid thrust gears;

controlling the sheath fluid supply unit to supply the sheath fluid to the sheath fluid receiving member at the propulsion pressure of the second sheath fluid thrust gear in the at least two sheath fluid thrust gears; the propulsion pressure of the first sheath fluid thrust gear is smaller than that of the second sheath fluid thrust gear.

Wherein the sheath liquid supply unit comprises a first sheath liquid pipeline and a second sheath liquid pipeline, and the first sheath liquid pipeline and the second sheath liquid pipeline are respectively connected with the sheath liquid receiving piece;

the controller is further configured to:

firstly controlling the sheath liquid to be supplied to the sheath liquid receiving part by the second sheath liquid pipeline at the propulsion pressure of the first sheath liquid thrust gear; and controlling the sheath liquid to be supplied to the sheath liquid receiving member at least by the first sheath liquid pipeline under the pushing pressure of the second sheath liquid pushing gear.

Wherein the model of the components composing the sheath liquid supply unit is different from the model of the components at least partially corresponding to the model of the components composing the propulsion liquid supply unit;

The parameters of the components constituting the sheath fluid supply unit are different from those of the components corresponding at least in part to those of the components constituting the propellant fluid supply unit.

Wherein the propellant liquid supply unit comprises a first propellant liquid pipeline and a second propellant liquid pipeline;

the length of the secondary sheath fluid line is different from the length of the secondary propellant fluid line,

and/or the pipe diameter of the second sheath liquid pipeline is smaller than that of the second propellant liquid pipeline.

The propulsion liquid supply unit comprises a first propulsion liquid pipeline, a second propulsion liquid pipeline and a sample propulsion unit, when a sample is detected, the first propulsion liquid pipeline and the second propulsion liquid pipeline are closed, valves between the main pipeline and all the sample reaction units are closed, and the sample propulsion unit is used for propelling propulsion liquid to the main pipeline.

Wherein the sheath fluid supply unit is configured to supply the sheath fluid to the sheath fluid receiver at a pushing pressure of the first sheath fluid pushing force range when a valve between the main pipe and the sample reaction unit is closed,

wherein,,

the continuous opening time of the second sheath liquid pipeline is greater than N times of the average continuous pushing time of the sample pushing units, wherein N is the number of the at least two sample reaction units, and N is greater than or equal to 2.

Wherein the sheath liquid supply unit includes a first sheath liquid pipeline and a second sheath liquid pipeline connected in parallel with each other;

the pipe diameter of the first sheath liquid pipeline is larger than that of the second sheath liquid pipeline,

and/or the tube length of the first sheath liquid pipeline is smaller than the tube length of the second sheath liquid pipeline;

the first sheath liquid pipeline and the second sheath liquid pipeline can be switched on and off independently of each other, and the sheath liquid is supplied to the sheath liquid receiving member through the first sheath liquid pipeline and/or the second sheath liquid pipeline in the on state;

the propellant liquid supply unit comprises a first propellant liquid pipeline and a second propellant liquid pipeline which are connected in parallel;

the pipe diameter of the first propellant liquid pipeline is larger than that of the second propellant liquid pipeline,

and/or the tube length of the first propellant liquid pipeline is smaller than the tube length of the second propellant liquid pipeline;

the first propellant line and the second propellant line can be switched on and off independently of one another, the propellant being supplied to the sample receiving piece via the first propellant line and/or the second propellant line in the on state.

The beneficial effects of this application: unlike the prior art, the blood cell analysis apparatus of the present application comprises a flow chamber, a main line, at least two sample reaction units, a sheath fluid supply unit and a propellant fluid supply unit. The sample reaction unit is used for conveying unused sample liquid to the main pipeline, the sheath liquid supply unit supplies sheath liquid to the sheath liquid receiving part of the flow chamber in at least two sheath liquid thrust steps, and the propellant liquid supply unit supplies propellant liquid to the main pipeline in at least two propellant liquid thrust steps so as to push the sample liquid in the main pipeline to the sample receiving part of the flow chamber through the propellant liquid. Wherein, sheath liquid supply unit's two at least sheath liquid thrust gear and propulsion liquid supply unit's two at least propulsion liquid thrust gear mutually support to realize impeing sheath liquid and propulsion liquid with different propulsion pressures, satisfy multiple detection channel and impel the propulsion demand of the propulsion liquid of different grade type sample liquid and the sheath liquid propulsion demand that corresponds, improve blood cell analysis equipment's practicality and detection accuracy, improve the user and experience the blood cell analysis equipment's of this application.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:

FIG. 1 is a schematic view showing the structure of a first embodiment of a blood cell analysis apparatus of the present application;

FIG. 2 is a schematic view of a second embodiment of a blood cell analysis apparatus according to the present application;

FIG. 3 is a schematic view showing the structure of a first embodiment of a sample liquid supply unit of the present application;

FIG. 4 is a schematic view showing the structure of a first embodiment of a sheath fluid supply unit of the present application;

FIG. 5 is a schematic view showing the structure of a third embodiment of the blood cell analysis apparatus of the present application.

Reference numerals: a blood cell analysis device A; a flow chamber 1; a sample receiving member 11; a sheath fluid receiving member 12; a main pipeline 2; a sample reaction unit 3; a sheath fluid supply unit 4; a primary sheath fluid line 41; a secondary sheath fluid line 42; a propellant liquid supply unit 5; a first propellant line 51; a second propellant line 52; a sample pushing unit 53.

Detailed Description

The present application is described in further detail below with reference to the drawings and examples. It is specifically noted that the following examples are only for illustration of the present application, but do not limit the scope of the present application. Likewise, the following embodiments are only some, but not all, of the embodiments of the present application, and all other embodiments obtained by one of ordinary skill in the art without making any inventive effort are within the scope of the present application.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.

In the description of the present application, it is to be understood that the terms "mounted," "configured," "connected," and "connected" are to be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected, unless explicitly stated and defined otherwise; the connection can be mechanical connection or electric connection; may be directly connected or may be connected via an intermediate medium. It will be apparent to those skilled in the art that the foregoing is in the specific sense of this application.

Referring to fig. 1, fig. 1 is a schematic structural view of a first embodiment of a blood cell analysis apparatus according to the present application. The blood cell analysis apparatus a provided in the embodiment of the present application includes a flow chamber 1, a main pipe 2, at least two sample reaction units 3, a sheath fluid supply unit 4, and a propellant fluid supply unit 5.

Wherein, flow cell 1 includes sample receiving part 11 and sheath liquid receiving part 12, sample receiving part 11 is used for receiving the sample liquid, sheath liquid receiving part 12 is used for receiving the sheath liquid, the outside of sample receiving part 11 can be located to sheath liquid receiving part 12 cover, the sheath liquid through sheath liquid receiving part 12 output, wrap up the sample liquid of sample receiving part 11 output, form sheath liquid sample laminar flow in flow cell 1, wherein, sheath liquid sample laminar flow includes two laminar flows, the sample liquid laminar flow that is located the inlayer and the sheath liquid laminar flow that wraps up the inlayer, for the sample liquid laminar flow of inlayer, the sheath liquid laminar flow is the laminar flow that is located the inlayer. At least two sample reaction units 3 are connected with the main pipeline 2, and the sample reaction units 3 are connected with the sample receiving piece 11 through the main pipeline 2, and at least two sample reaction units 3 are used for respectively preparing different types of sample liquid, selectively communicate with the main pipeline 2 respectively, and time-sharing conveying the different types of sample liquid into the main pipeline 2.

In the present embodiment, the sample reaction unit 3 selectively communicates with the main line 2, and not a part of the sample reaction unit 3 is connected to the main line 2, but a part of the sample reaction unit 3 is not connected to the main line 2. All the sample reaction units 3 are connected to the main pipeline 2, and valves for controlling whether the sample reaction units 3 are filled with sample liquid or not are arranged at liquid outlets where the sample reaction units 3 are connected with the main pipeline 2, and when the valves are opened, the sample reaction units 3 are filled with the sample liquid into the main pipeline 2, and are in a communication state at the moment; when the valve is closed, the sample reaction unit 3 cannot inject the sample liquid into the main pipe 2, and is in a non-communication state. The at least two sample reaction units 3 are used for being respectively and selectively communicated with the main pipeline 2, in practice, valves on the at least two sample reaction units 3 are respectively and selectively opened, at most one valve is opened at a time, sample liquid pollution caused by the simultaneous occurrence of more than two different sample liquids in the main pipeline is prevented, the at least two sample reaction units 3 share the flow chamber 1, one sample liquid is detected each time, and the cost of the whole machine is saved.

Wherein the sheath fluid supply unit 4 is connected to the sheath fluid receiving member 12 of the flow chamber 1 for supplying sheath fluid to the sheath fluid receiving member 12 in at least two sheath fluid thrust steps; the propellant liquid supply unit 5 is connected to the sample receiving member 11 via the main line 2 for supplying propellant liquid to the main line 2 in at least two propellant liquid thrust steps for propelling the sample liquid in the main line 2 into the sample receiving member 11 via the propellant liquid.

The propellant liquid in the embodiment of the application may be a solvent which does not cause a large scale of pollution to the sample liquid, such as a cleaning solution or a diluent, and does not damage cells in the sample liquid, and other types of solvents may be selected as the propellant liquid. In the main line 2, the sample liquid in the main line 2 is pushed toward the flow chamber 1 by pushing of the pushing liquid supply unit 5.

In the present embodiment, the sheath fluid supply unit 4 includes at least two sheath fluid thrust steps, the propulsion fluid supply unit 5 also includes at least two propulsion fluid supply steps, the at least two sheath fluid thrust steps of the sheath fluid supply unit 4 include a first sheath fluid thrust step and a second sheath fluid thrust step, and the at least two propulsion fluid thrust steps of the propulsion fluid supply unit 5 include a first propulsion fluid thrust step and a second propulsion fluid thrust step. In the process of detecting one sample liquid, the sheath liquid supply unit 4 can use the first sheath liquid thrust gear to push the sheath liquid, and the propulsion liquid supply unit 5 can use the first propulsion liquid thrust gear to push the sample liquid; alternatively, the sheath fluid supply unit 4 uses a second sheath fluid thrust gear to push the sheath fluid, and the propellant fluid supply unit 5 uses a second propellant fluid thrust gear to push the sample fluid; alternatively, the sheath fluid supply unit 4 uses a first sheath fluid thrust gear to propel the sheath fluid, and the propulsion fluid supply unit 5 uses a second propulsion fluid thrust gear to propel the sample fluid; alternatively, the sheath fluid supply unit 4 uses the second sheath fluid thrust gear to propel the sheath fluid, and the propulsion fluid supply unit 5 uses the first propulsion fluid thrust gear to propel the sample fluid. That is, the thrust gears of the sheath fluid supply unit 4 and the propulsion fluid supply unit 5 are matched with each other to form at least two gear combinations (one gear combination is calculated if the ratio of the actual values is the same), so that the requirements of different detection channels on the propulsion pressure of the sample fluid and the propulsion pressure of the sheath fluid are satisfied.

In addition, by matching different gears, the change of large flow speed and large flow rate of the liquid can be smoothly changed by utilizing the gear switching of the sheath liquid supply unit 4 and the propelling liquid supply unit 5, the disturbance of the liquid path is avoided, and the condition that the sample liquid is diluted in the flow chamber is reduced. For example, when a new detection channel is switched (i.e. a new sample reaction unit 3 is switched to inject another type of sample liquid), the sheath liquid supply unit 4 needs to be switched from a first sheath liquid thrust gear to a second sheath liquid thrust gear, the propellant liquid supply unit 5 needs to be switched from the first propellant liquid thrust gear to the second propellant liquid thrust gear, if the gears of the sheath liquid supply unit 4 and the propellant liquid supply unit 5 are directly and simultaneously switched, the instantaneous flow rate of the liquid path in the main pipeline 2 and the flow chamber 1 is suddenly changed, the liquid path is disturbed, the detection accuracy of the blood cell analysis equipment A is affected, and the speed of the whole blood cell analysis equipment A is reduced.

If the thrust gear of the sheath fluid supply unit 4 or the thrust gear of one of the propulsion fluid supply units 5 is switched, the thrust gear of the other propulsion fluid supply unit 5 or the sheath fluid supply unit 4 is switched after the fluid path is stable, so that the switching of the thrust gears of the sheath fluid supply unit 4 and the propulsion fluid supply unit 5 is realized, the detection design requirement is met, the fluid path disturbance caused by the mutation of the fluid path flow velocity does not occur, the detection accuracy of the blood cell analysis equipment A is influenced, and the detection efficiency of the blood cell analysis equipment A is improved.

In summary, the blood cell analysis device a provided by the embodiment of the present application includes a sheath fluid supply unit 4 having at least two sheath fluid thrust steps, and a propellant fluid supply unit 5 having at least two propellant fluid thrust steps, and through the mutual cooperation of the sheath fluid supply unit 4 and the propellant fluid supply unit 5, the propelling pressure requirements of different detection channels on the sheath fluid and the sample fluid are satisfied, and the practicality and the detection efficiency of the blood cell analysis device a are improved.

Optionally, the blood cell analysis apparatus a further comprises a controller (not shown).

The controller is used for: during the laminar flow of the sheath fluid sample, the propulsion fluid supply unit 5 is controlled to push the propulsion fluid in at least two propulsion fluid thrust gears with the propulsion pressure of the first propulsion fluid thrust gear, for example, the propulsion fluid is powered by a positive pressure source, or the propulsion fluid is powered by a combination of the positive pressure source and the injector. And after the laminar flow of the sheath fluid sample is formed, the propellant fluid supply unit 5 is controlled to push the propellant fluid in at least two propellant fluid thrust steps at the propellant pressure of the second propellant fluid thrust step so as to push the sample fluid from the main pipe 2 into the sample receiving member 11. Wherein the propulsion pressure of the first propulsion liquid thrust gear is greater than the propulsion pressure of the second propulsion liquid thrust gear.

In the present embodiment, in the process of forming the sheath fluid sample laminar flow, the sheath fluid pushed by the sheath fluid supply unit 4 forms a stable pure sheath fluid layer flow in the flow chamber 1, and therefore, the pushing pressure of the first pushing fluid pushing force gear is required to be large, and the pushing sample fluid breaks through the pure sheath fluid layer flow in the flow chamber 1 to form the sheath fluid sample laminar flow. After the laminar flow of the sheath fluid sample is formed, the flow rate of the sample fluid is only required to be matched with the flow rate of the pure sheath fluid, so that the propulsion fluid supply unit 5 is switched to the second propulsion fluid thrust gear to propel the sample fluid at a propulsion pressure smaller than that of the first propulsion fluid thrust gear, and the laminar flow of the sheath fluid sample is kept stable, for example, the laminar flow of the sheath fluid sample is powered by using the syringe as a power source only, or the laminar flow of the sheath fluid sample is powered by using the positive pressure source only as a power source, or the laminar flow of the sheath fluid is powered by using the positive pressure source and the syringe as a power source and combining the positive pressure source with the negative pressure provided by the return stroke of the syringe. On the basis of keeping the laminar flow of the sheath liquid sample stable, the energy consumption of the propelling liquid supply unit 5 can be reduced, and the cost is reduced. Under the condition that other conditions are unchanged, the propulsion pressure of the first propulsion liquid thrust gear is larger than that of the second propulsion liquid thrust gear.

Alternatively, referring to fig. 2 and 3, fig. 2 is a schematic structural view of a second embodiment of the blood cell analysis apparatus of the present application, and fig. 3 is a schematic structural view of a first embodiment of the propellant supply unit of the present application. The propellant liquid supply unit 5 comprises a first propellant liquid line 51 and a second propellant liquid line 52, the first propellant liquid line 51 and the second propellant liquid line 52 being connected to the main line 2, respectively.

Wherein, during the formation of the sheath fluid sample laminar flow, the controller controls the propelling fluid to be propelled by the propelling pressure of the first propelling fluid propelling gear through at least the first propelling fluid pipeline 51. And after laminar flow of the sheath fluid sample is established, the controller controls the propulsion of the propulsion fluid through the secondary propulsion fluid line 52 at the propulsion pressure of the secondary propulsion fluid thrust gear.

In this embodiment, the pipe diameter of the first propellant line 51 may be larger than the pipe diameter of the second propellant line 52, or the pipe length of the first propellant line 51 may be shorter than the pipe diameter of the second propellant line 52, so that when the propellant is propelled by the same propulsion pressure, the flow rate of the propellant in the first propellant line 51 is larger than the flow rate in the second propellant line 52 due to the pipe resistance or the along-the-way pressure drop. During the formation of a laminar flow of sheath fluid sample, the propellant fluid supply unit 5 is required to push the sample fluid at a greater propellant pressure (it will be appreciated that the greater herein and hereinafter lesser are not absolute magnitudes with respect to the propellant pressures of the different propellant steps) to break through the laminar flow of sheath fluid sample to form a laminar flow of sheath fluid sample, and the controller may therefore control the propellant fluid supply unit 5 to push the sample fluid to the sample receiving member 11 via the first propellant fluid line 51 in a combination of the propellant pressures of the first propellant fluid propellant steps.

In other embodiments, to obtain a greater propulsion pressure, the controller may also control the propulsion liquid supply unit 5 to propel the sample liquid towards the sample receiving member 11 in a combination of propulsion pressures of the first propulsion liquid propulsion stage via the first propulsion liquid line 51 and the second propulsion liquid line 52, i.e. a propulsion pressure that is greater than the propulsion pressure provided in a combination of propulsion pressures of the first propulsion liquid propulsion stage via the first propulsion liquid line 51.

After the laminar flow of the sheath fluid sample is formed, the propellant fluid supply unit 5 can propel the sample fluid with a small propelling pressure, and the controller controls the propellant fluid supply unit 5 to continuously and smoothly propel the sample fluid into the sample receiving member 11 with the propelling pressure of the second propellant fluid propulsion gear through the second propellant fluid line 52.

In other embodiments, in order to meet the detection design requirements of different detection channels, during the formation of the sheath fluid sample laminar flow, the propulsion fluid supply unit 5 is controlled to push the propulsion fluid through the first propulsion fluid pipeline 51 in the form of the combination of the propulsion pressures of the first propulsion fluid thrust gear, or the propulsion fluid supply unit 5 is controlled to push the propulsion fluid through the first propulsion fluid pipeline 51 and the second propulsion fluid pipeline 52 in the form of the combination of the propulsion pressures of the first propulsion fluid thrust gear, and then the propulsion fluid supply unit 5 can also push the sample fluid through the second propulsion fluid pipeline 52 in the form of the propulsion pressures of the first propulsion fluid thrust gear; or, in the process of forming the sheath fluid sample laminar flow, the propulsion fluid supply unit 5 is controlled to push the propulsion fluid through the first propulsion fluid pipeline 51 by the propulsion pressure provided by the combination of the propulsion pressures of the first propulsion fluid thrust gear, and push the sample fluid through the first propulsion fluid pipeline 51 by the propulsion pressure of the second propulsion fluid thrust gear; or, in the process of forming the sheath fluid sample laminar flow, the propulsion fluid supply unit 5 is controlled to push the propulsion fluid through the first propulsion fluid pipeline 51 and the second propulsion fluid pipeline 52 in the propulsion pressure provided by the combination of the propulsion pressures of the first propulsion fluid thrust gear, and then push the sample fluid through the first propulsion fluid pipeline 51 and the second propulsion fluid pipeline 52 in the propulsion pressure of the second propulsion fluid thrust gear. That is, in the embodiment of the present application, the propellant supply unit 5 may achieve more selection of the pushing pressure by the cooperation of fewer pushing steps with the propellant line. The selected thrust gear and the propellant liquid pipeline can be determined according to the detection design requirements of different detection channels of the blood cell analysis equipment A on the pushing pressure of the sample liquid, and the application is not limited to the pushing pressure.

Further, the propellant liquid supply unit 5 may further push the propellant liquid to clean the sample reaction unit 3 after the sample liquid is detected. During the cleaning of the sample reaction unit 3, the controller controls the pushing liquid supply unit 5 to push the pushing liquid (here, the pushing liquid is the cleaning liquid) through at least the first pushing liquid pipeline 51 at the pushing pressure of the third pushing liquid pushing step of the at least two pushing liquid pushing steps to clean the sample reaction unit 3.

In this embodiment, the sample reaction unit 3 is cleaned, and cells are attached to the wall of the sample reaction unit 3, so that the cleaning is difficult, and a large pushing pressure is required to push the pushing liquid, so as to achieve the cleaning effect of the sample reaction unit 3. Therefore, the propellant supply unit 5 pushes the propellant at the propulsion pressure of the third propellant thrust stage through at least the first propellant line 51.

If the propulsion pressure of the third propulsion liquid thrust gear is greater than the propulsion pressure of the second propulsion liquid thrust gear and less than the propulsion pressure of the first propulsion liquid thrust gear, the propulsion liquid supply unit 5 may push the propulsion liquid into the sample reaction unit 3 through the first propulsion liquid pipeline 51 and the second propulsion liquid pipeline 52. Alternatively, if the propulsion pressure of the third propulsion fluid propulsion gear is greater than the propulsion pressure of the first propulsion fluid propulsion gear, the propulsion fluid supply unit 5 may propel the propulsion fluid only through the first propulsion fluid line 51. In other embodiments, the propellant supply unit 5 can also be pushed in with propellant via the first propellant line 51 and the second propellant line 52. The present application is not limited to this on the premise of realizing the cleaning effect of the sample reaction unit 3.

In summary, the propellant supply unit 5 comprises a first propellant line 51 and a second propellant line 52, and the propellant is pushed in at least two propellant thrust steps. That is, the propulsion liquid supply unit 5 can realize that more propulsion pressure selections are realized with fewer propulsion liquid through the collocation between different propulsion liquid thrust gear and different propulsion liquid pipelines, so that the propulsion pressure requirements of different detection channels in the blood cell analysis equipment A on the sample liquid are met, and the practicability and the detection efficiency of the blood cell analysis equipment A are improved.

Optionally, with continued reference to fig. 2, the controller is further configured to control the sheath fluid supply unit 4 to supply the sheath fluid to the sheath fluid receiving member 12 at the propulsion pressure of the first sheath fluid thrust gear among the at least two sheath fluid thrust gears, so as to form a pure sheath fluid laminar flow in the flow chamber 1. The laminar flow of pure sheath fluid may be established before the sample fluid is output to the sample receiving member 11; the laminar flow of pure sheath fluid may also be formed before the sample fluid enters the detection zone when the sample fluid is output to the sample receiving member 11; a laminar flow of pure sheath fluid may also be established when the sample fluid is just delivered to the sample receiving member.

The sheath fluid supply unit 4 is controlled again to supply sheath fluid to the sheath fluid receiving member 12 at the advancing pressure of the second sheath fluid thrust gear in at least two sheath fluid thrust gears to form a sheath fluid sample laminar flow in the flow chamber 1. Under the condition that other conditions are not changed, the propulsion pressure of the first sheath fluid thrust gear is smaller than that of the second sheath fluid thrust gear.

Wherein, in order to form a laminar flow of the pure sheath liquid before the sample liquid is output from the sample receiving member 11, the sheath liquid supply unit 4 supplies the sheath liquid to the sheath liquid receiving member 12 in advance to form a laminar flow of the pure sheath liquid in the flow chamber 1. If the sheath liquid is pushed by a large pushing pressure in the initial stage of the laminar flow of the sheath liquid, the pressure change is too large, and thus a stable laminar flow of the sheath liquid cannot be formed rapidly. Therefore, the controller firstly controls the sheath fluid supply unit 4 to push the sheath fluid in a first sheath fluid thrust gear with smaller pushing pressure, and after the flow of the pure sheath fluid is stable, the controller pushes the sheath fluid in a second sheath fluid thrust gear with larger pushing pressure.

After the formation of the pure sheath fluid flow, the sheath fluid supply unit 4 pushes the sheath fluid with the pushing pressure of the second sheath fluid pushing gear, and the sample fluid is output by the sample receiving member 11, so that the sheath fluid having a large pressure squeezes the sample fluid, so that cells are arranged one by one in the formed sheath fluid sample flow.

Alternatively, as shown in fig. 2 and 4, fig. 4 is a schematic structural view of a first embodiment of the sheath fluid supply unit of the present application. The sheath fluid supply unit 4 includes a first sheath fluid pipe 41 and a second sheath fluid pipe 42, and the first sheath fluid pipe 41 and the second sheath fluid pipe 42 are connected to the sheath fluid receiver 12, respectively.

The pipe diameter of the first sheath fluid pipe 41 may be larger than that of the second sheath fluid pipe 42, so that the flow rate of the sheath fluid in the first sheath fluid pipe 41 is larger than that in the second sheath fluid pipe 42 under the propulsion pressure of the same sheath fluid thrust gear.

The controller controls the sheath liquid supply unit 4 to supply sheath liquid to the sheath liquid receiving member 12 through the second sheath liquid pipeline 42 at the advancing pressure of the first sheath liquid pushing gear so as to form a laminar flow of pure sheath liquid in the flow chamber 1. And after the flow of the pure sheath liquid is stabilized, when the sample liquid is output to the sample receiving member 11 or when the sample liquid is ready to be output to the sample receiving member 11, the controller starts to control the sheath liquid supply unit 4 to supply the sheath liquid to the sheath liquid receiving member 12 at least through the first sheath liquid pipe 41 with the advancing pressure of the second sheath liquid pushing force gear, and continuously supply the sheath liquid to the sheath liquid receiving member 12 through the first sheath liquid pipe 41 with the advancing pressure of the second sheath liquid pushing force gear when the sample liquid is output to the sample receiving member 11, so that the sheath liquid sample laminar flow is formed in the flow chamber 1.

In the present embodiment, after the laminar flow of the pure sheath liquid is formed in the flow chamber 1, the sheath liquid supply unit 4 pushes the sheath liquid with a larger pushing pressure, so that the sample liquid and the sheath liquid layer flow form a laminar flow of the sheath liquid sample. Therefore, after the sheath liquid supply unit 4 supplies the sheath liquid to the sheath liquid receiver 12 at the advancing pressure of the first sheath liquid thrust range through the second sheath liquid piping 42 and forms a laminar flow of the pure sheath liquid in the flow chamber 1, the sheath liquid supply unit 4 may be switched to advance the sheath liquid at the advancing pressure of the second sheath liquid thrust range only through the first sheath liquid piping 41 in order to achieve a larger advancing pressure; alternatively, the sheath fluid is pushed by the pushing pressure of the second sheath fluid pushing gear through the first sheath fluid pipeline 41 and the second sheath fluid pipeline 42; alternatively, the sheath fluid is pushed by the pushing pressure of the first sheath fluid pushing step through the first sheath fluid pipeline 41 and the second sheath fluid pipeline 42, so that the sheath fluid is pushed by the larger pushing pressure after the laminar flow of the pure sheath fluid is formed.

In sum, the sheath liquid supply unit 4 is matched with different pipelines and different thrust gears, so that the sheath liquid supply unit 4 can realize the selection of more propelling pressure of the sheath liquid with fewer thrust gears, the requirement of different detection channels on the propelling pressure of the sheath liquid is met, and the detection efficiency of the blood cell analysis equipment A is improved.

Wherein the model of the components constituting the sheath liquid supply unit 4 is different from the model of the components constituting the propellant liquid supply unit 5, which at least partially corresponds to the model; the parameters of the components constituting the sheath fluid supply unit 4 are different from those of the components corresponding at least in part to those of the components constituting the propellant fluid supply unit 5.

That is, the power source providing the propulsion pressure in the sheath fluid supply unit 4 may be of a different type than the power source providing the propulsion pressure in the propulsion fluid supply unit 5, so that the propulsion pressure of the different sheath fluid thrust steps may be wholly or partly different from the propulsion pressure of the different propulsion fluid thrust steps, for example, the propulsion pressure of the first sheath fluid thrust step may be equal to or different from the propulsion pressure of the second propulsion fluid thrust step, and the propulsion pressure of the second sheath fluid thrust step may be equal to or different from the propulsion pressure of the first propulsion fluid thrust step. Thereby different sheath liquid thrust gears are matched with different propelling liquid thrust gears randomly, further multi-gear of blood cell analysis equipment A is realized, thereby the requirement of the detection design requirement of more different detection channels on the propelling pressure of sample liquid and sheath liquid can be met, the practicability of the blood cell analysis equipment A is improved, and when the gears are switched, liquid flow is smoother, and turbulence is not easy to occur due to gear or pressure mutation.

Further, the pipe diameters between the first sheath liquid pipe 41 and the second sheath liquid pipe 42 of the sheath liquid supply unit 4 may be different, and the pipe diameters of the first propellant liquid pipe 51 and the second propellant liquid pipe 52 of the propellant liquid supply unit 5 may be different; and the tube diameters of the sheath liquid pipeline and the propelling liquid pipeline can be different.

Therefore, the sheath liquid supply unit 4 can push sheath liquid through a single sheath liquid pipeline with one sheath liquid thrust gear or push sheath liquid through a plurality of sheath liquid pipelines with one sheath liquid thrust gear, so that a plurality of pushing pressures of one sheath liquid thrust gear of the sheath liquid supply unit 4 are realized, the multi-gear of the pushing pressure provided by the sheath liquid supply unit 4 is reflected, and in gear switching, liquid flow is smoother and turbulence is not easy to occur due to gear or pressure mutation. The propulsion liquid supply unit 5 pushes propulsion liquid through a single different propulsion liquid pipeline by one propulsion liquid thrust gear or pushes propulsion liquid through a plurality of propulsion liquid pipelines by one propulsion liquid thrust gear, so that a plurality of propulsion pressures of one propulsion liquid thrust gear of the propulsion liquid supply unit 5 are realized, multiple gears of the propulsion pressure provided by the propulsion liquid supply unit 5 are reflected, and when the gears are switched, liquid flow is smoother and turbulence is not easy to occur due to the gears or pressure mutation.

Further, if the sheath fluid supply unit 4 and the propellant fluid supply unit 5 are identical in model number and parameters of all components, the resulting propulsion pressures of the respective gear stages are identical. For example, the propulsion pressure of the second sheath fluid thrust gear through the first sheath fluid line 41 is 2KPa, and the propulsion pressure of the second sheath fluid thrust gear through the first sheath fluid line 41 and the second sheath fluid line 42 is 4KPa; the propulsion pressure of the first propulsion liquid through the first propulsion liquid line 51 is 2KPa, and the propulsion pressure of the first propulsion liquid through the first propulsion liquid line 51 and the second propulsion liquid line 52 is 4KPa. After the formation of the pure sheath fluid layer flow, the ratio of the propelling pressure of the sheath fluid supply unit 4 to the propelling pressure of the propelling fluid supply unit 5 to the sample fluid may be 2:2, 4:4, 2:4, 4:2, that is, 1:1, 1:2, and 2:1 before the formation of the sheath fluid sample flow.

If the types and parameter parts of all the components of the sheath fluid supply unit 4 and the propulsion fluid supply unit 5 are different, the propulsion pressures of the respective gear stages are different. For example, the propulsion pressure of the second sheath fluid thrust gear through the first sheath fluid line 41 is 2KPa, and the propulsion pressure of the second sheath fluid thrust gear through the first sheath fluid line 41 and the second sheath fluid line 42 is 4KPa; the propulsion pressure of the first propulsion liquid through the first propulsion liquid line 51 is 5KPa and the propulsion pressure of the first propulsion liquid through the first propulsion liquid line 51 and the second propulsion liquid line 52 is 7KPa. After the pure sheath liquid layer flow is formed, the ratio of the propelling pressure of the sheath liquid supply unit 4 to the propelling pressure of the propelling liquid supply unit 5 can be 2:5, 4:7, 2:7 and 4:5 before the sheath liquid sample flow is formed, and the ratio of the propelling pressure to the propelling pressure of the sheath liquid can be four, so that the method can be suitable for the requirements of the detection design requirements of more detection channels on the propelling pressures of the sample liquid and the sheath liquid.

Therefore, in the embodiment of the application, the model and the parameters of the sheath liquid supply unit 4 and the part of the components of the propulsion liquid supply unit 5 are different, so that the sheath liquid supply unit 4 and all propulsion pressures of the propulsion liquid supply unit 5 are matched, the requirements of different detection channels on the propulsion pressures of the sample liquid and the sheath liquid can be met, the multi-gear of the blood cell analysis equipment A is realized, the liquid flow is smoother when the gears are switched, turbulence is not easy to occur due to the gears or the pressure mutation, the practicability of the blood cell analysis equipment A is improved, and the use experience of a user on the blood cell analysis equipment A in the embodiment of the application is improved.

Alternatively, referring to fig. 3 and 4, the propellant liquid supply unit 5 includes a first propellant liquid line 51 and a second propellant liquid line 52, and the sheath liquid supply unit 4 includes a first sheath liquid line 41 and a second sheath liquid line 42.

Wherein the length of the secondary sheath fluid conduit 42 is different from the length of the secondary propellant conduit 52. For example, the length of the secondary sheath fluid conduit 42 is greater than the length of the secondary propellant conduit 52. In one embodiment, the length of the inlet terminal tubing of the secondary sheath fluid tubing 42 is greater than the length of the inlet terminal tubing of the secondary propellant fluid tubing 52.

In this embodiment, since the sheath fluid is used to assist in pushing the sample fluid, that is, the sheath fluid is continuously pushed into the sheath fluid receiving member 12, the sample fluid is intermittently fed into the sample receiving member 11 during the continuous sample detection. If the pushing pressure of the sheath fluid is too high, when the sample fluid is not inputted into the sample receiving member 11, a problem due to a pressure difference easily occurs, and the sheath fluid flows back into the sample receiving member 11. The length of the inlet terminal line of the secondary sheath fluid line 42 is designed to be greater than the length of the inlet terminal line of the secondary propellant line 52. The longer the length of the pipeline is, the larger the pipe resistance of the pipeline is under the same condition, so that the along-path pressure drop of the propelling pressure provided by the sheath liquid supply unit 4 is larger than the along Cheng Yajiang of the propelling pressure provided by the propelling liquid supply unit 5, the pressure difference of the liquid in the flow chamber 1 is reduced, the backflow of the sheath liquid is avoided, and the safety of the blood cell analysis equipment A is improved.

In another embodiment, the tube diameter of the secondary sheath fluid tube 42 is different than the tube diameter of the secondary propellant tube 52. For example, the tube diameter of the secondary sheath fluid conduit 42 is smaller than the tube diameter of the secondary propellant conduit 52. In one embodiment, the inlet terminal tubing of the secondary sheath fluid tubing 42 has a smaller tubing diameter than the inlet terminal tubing of the secondary propellant fluid tubing 52.

In order to realize that the along-path pressure drop of the propelling pressure provided by the sheath liquid supply unit 4 is larger than that provided by the propelling liquid supply unit 5, besides the lengths of the sheath liquid pipeline and the propelling liquid pipeline, the pipe diameters of the sheath liquid pipeline and the propelling liquid pipeline can be designed. Since the smaller the pipe diameter of the pipe is, the larger the pipe resistance of the pipe is in the same case, in another embodiment of the present application, it can be designed as: the inlet terminal line of the second sheath fluid line 42 has a smaller pipe diameter than the inlet terminal line of the second propellant line 52 to prevent backflow of the sheath fluid and improve the safety of the blood cell analysis apparatus A.

In another embodiment, the length of the inlet terminal line of the second sheath fluid line 42 may be longer than the inlet terminal line of the second propellant line 52, and the pipe diameter of the inlet terminal line of the second sheath fluid line 42 may be smaller than the pipe diameter of the inlet terminal line of the second propellant line 52. In other embodiments, the length of the outlet terminal line of the second sheath fluid line 42 may be greater than the outlet terminal line of the second propellant fluid line 52, and the pipe diameter of the outlet terminal line of the second sheath fluid line 42 may be smaller than the pipe diameter of the outlet terminal line of the second propellant fluid line 52.

Optionally, referring to fig. 5, fig. 5 is a schematic structural diagram of a third embodiment of the blood cell analysis apparatus of the present application. The propellant liquid supply unit 5 further comprises a sample propellant unit 53.

In this embodiment, the sample pushing unit 53 may be a syringe. At the time of sample detection, that is, the sample liquid has entered the main line 2 from the sample reaction unit 3, the valves between the main line 2 and all the sample reaction units 3 are closed. After the sheath fluid sample laminar flow is established, the propellant fluid supply unit 5 propels the sample fluid at a lower propellant pressure, which in the embodiment without the sample propulsion unit 53 is achieved by the second propellant fluid line 52 at the propellant pressure of the second propellant fluid thrust stage provided by the positive pressure source.

In the embodiment with the sample propulsion unit 53, the first propulsion liquid pipeline 51 and the second propulsion liquid pipeline 52 may be closed, only the sample propulsion unit 53 is left to propel the sample liquid with a smaller propulsion pressure, and at this time, when the propulsion pressure of the corresponding second propulsion liquid propulsion gear is the propulsion pressure provided by the injector only when the first propulsion liquid pipeline 51 and the second propulsion liquid pipeline 52 are both closed, the propulsion pressure provided by the injector only is smaller than the propulsion pressure provided by the second propulsion liquid propulsion gear provided by the Yu Zhengya source; since the pushing pressure of the sample pushing unit 53 is relatively stable and small, the cells in the sample liquid can pass through the flow chamber 1 slowly one by one, and are accurately counted, so that the detection accuracy of the blood cell analysis device a is improved.

Meanwhile, the power sources for providing propulsion pressure in the first propulsion liquid pipeline 51, the second propulsion liquid pipeline 52 and the propulsion liquid supply unit 5 are closed, so that the energy loss of devices is effectively saved, and the cost is saved.

In another embodiment, before the sheath fluid sample laminar flow is formed, the propulsion fluid supply unit 5 may start the first propulsion fluid thrust gear to push the propulsion fluid, and at the same time, may start the sample propulsion unit 53, where the sample propulsion unit 53 pushes the propulsion fluid together with a power source that provides the propulsion pressure of the first propulsion fluid thrust gear, so as to increase the propulsion pressure of the sample fluid, and at the same time, increase the selection of the propulsion pressure that can be provided by the propulsion fluid supply unit 5.

Further, when N sample reaction units 3 participate in sample analysis in the blood cell analysis device a, the first sample reaction unit 3 opens a valve, and the first sample liquid is injected into the main pipeline 2, so that the first sample liquid is subjected to sample detection, at this time, the valve between the main pipeline 2 and the sample reaction unit 3 is completely closed, so that the propellant liquid is prevented from entering the sample reaction unit 3 through the opened valve when the main pipeline 2 pushes the first sample liquid, and the sample liquid is polluted. After the sample detection of the first sample liquid is completed, the valve of the second sample reaction unit 3 is opened to inject the second sample liquid into the main pipeline 2, and the blood cell analysis device a performs the sample detection of the second sample liquid. Wherein N is the number of at least two sample reaction units 3, and N is 2 or more.

For the purpose of the stability of the liquid path and the detection stability of the blood cell analysis apparatus, a stable pure sheath liquid layer flow is formed in the flow chamber before the blood cell analysis is performed, but if after each sample liquid is detected, the sheath liquid supply unit 4 stops pushing the sheath liquid, and pushes the sheath liquid again to form a pure sheath liquid laminar flow before the next sample liquid is output to the sample receiving member 11, the time for waiting for the formation of the pure sheath liquid laminar flow is long, the time for continuously detecting a plurality of sample liquids is prolonged, and the detection efficiency of the blood cell analysis apparatus a is reduced. And the sheath fluid supply unit 4 is frequently turned on and off for a while, which is liable to damage the sheath fluid supply unit 4.

Therefore, the embodiment of the present application proposes that, in the process of continuously performing multiple sample liquid detection, after the detection of the first sample liquid is completed, the sheath liquid supply unit 4 continuously pushes the sheath liquid through the second sheath liquid pipeline 42 until the second sample liquid is conveyed to the sample receiving member 11, so that stable pure sheath liquid flow is continuously formed in the process of detecting multiple sample liquids, the time for waiting for the formation of the pure sheath liquid flow is reduced, the efficiency of detecting multiple sample liquids is improved, and the detection efficiency of the blood cell analysis apparatus a is improved.

Further, after the formation of the pure sheath liquid layer flow, the propellant liquid supply unit 5 propels the sample liquid to the sample receiving member 11 so that the sample liquid is subjected to sample detection. In practical applications, the amounts of the sample solutions injected into the main pipe 2 each time by the different sample reaction units 3 are the same, but the amounts of the sample detection performed by the different sample solutions are different. Therefore, if the actual amount of a certain sample liquid is smaller than the injection amount of the sample reaction unit 3, the remaining sample liquid is discharged from the flow cell 1 with the aid of the sheath liquid after the completion of the sample detection.

Thus, the continuous advance time of the sample advance unit 53 is a time from the start of the transfer of the sample liquid injected into the main line 2 to the completion of the detection of the sample liquid in the flow chamber 1. When the second sheath fluid line 42 of the sheath fluid supply unit 4 detects a sample, the average continuous pushing time of the second sheath fluid line 42 is the sum of the time for forming a laminar flow of the pure sheath fluid before the pure sheath fluid starts to be output to the sample receiving member 11, the time for the sample fluid to detect the sample, and the time for assisting the remaining sample fluid to be discharged out of the flow chamber 1. Therefore, when the plurality of sample liquids are detected, the opening timing of the second sheath liquid line 42 is earlier than the timing of the first sample liquid entering the sample receiving member 11, and the closing timing of the second sheath liquid line 42 is later than the timing of the last sample liquid completely exiting the flow chamber 1, during which the second sheath liquid line 42 is continuously opened, that is, the continuous opening time of the second sheath liquid line 42 is longer than the sum of the continuous pushing times of the plurality of sample pushing units 53. If N sample reaction units 3 participate in the sample detection, the sum of the continuous pushing times of the N sample pushing units 53 is also N times the average continuous pushing time of the sample pushing units 53. That is, the continuous open time of the secondary sheath fluid circuit 42 is greater than N times the average continuous advance time of the sample advance unit 53.

For example, when N is 4, that is, there are 4 sample reaction units 3 involved in the sample detection, the time from the injection of the sample liquid of the first sample reaction unit 3 to the completion of the sample detection is 10s, and the time for discharging the remaining sample liquid not involved in the sample detection is 4s; the time from the injection of the sample liquid to the completion of the sample detection in the second sample reaction unit 3 is 20s, and the time for discharging the remaining sample liquid is 2s; the time from the injection of the sample liquid to the completion of the sample detection in the third sample reaction unit 3 is 30s, and the time for discharging the remaining sample liquid is 1s; the time from the injection of the sample to the completion of the sample detection in the fourth sample reaction unit 3 was 40s, and there was no remaining sample liquid. At this time, the sample pushing units 53 correspond to continuous pushing times of 10s, 20s, 30s and 40s, and the average continuous pushing time of the sample pushing units 53 is 25s. The time for the second sheath fluid line 42 to open and deliver the sheath fluid until a laminar flow of the sheath fluid is formed is 5s, so that the continuous opening time of the second sheath fluid line 42 is 5s+10s+4s+20s+2s+30s+1s+40s is 112s. The average continuous advancing time of the sample advancing unit 53 is 4 times 100s, and thus the continuous opening time of the secondary sheath fluid piping 42 is greater than 4 times the average continuous advancing time of the sample advancing unit 53. That is, the continuous open time of the secondary sheath fluid circuit 42 is greater than N times the average continuous advance time of the sample advance unit 53.

Optionally, please continue to refer to fig. 3 and 4. The sheath fluid supply unit 4 includes a first sheath fluid pipe 41 and a second sheath fluid pipe 42 connected in parallel with each other.

Wherein, the pipe diameter of the first sheath liquid pipeline 41 is larger than that of the second sheath liquid pipeline 42. The larger the pipe diameter of the pipe is, the smaller the pipe resistance of the pipe is in the same case, so that when the sheath liquid is pushed in the same sheath liquid pushing step, the flow rate of the sheath liquid on the first sheath liquid pipe 41 is larger than the flow rate on the second sheath liquid pipe 42.

And/or the tube length of the first sheath fluid tube 41 is smaller than the tube length of the second sheath fluid tube 42. The larger the tube length of the tube is, the larger the tube resistance of the tube is in the same case, and therefore, when pushing the sheath liquid in the same sheath liquid pushing force gear, the flow rate of the sheath liquid on the first sheath liquid tube 41 is larger than the flow rate on the second sheath liquid tube 42.

It will be appreciated that in order to achieve a greater flow rate of sheath fluid on the primary sheath fluid conduit 41 than on the secondary sheath fluid conduit 42 when pushing the sheath fluid in the same sheath fluid thrust range. The tube diameter of the first sheath liquid tube 41 in the blood cell analysis apparatus a is larger than the tube diameter of the second sheath liquid tube 42, or the tube length of the first sheath liquid tube 41 is smaller than the tube length of the second sheath liquid tube 42, or the tube diameter of the first sheath liquid tube 41 is larger than the tube diameter of the second sheath liquid tube 42, and the tube length of the first sheath liquid tube 41 is smaller than the tube length of the second sheath liquid tube 42.

The first sheath liquid piping 41 and the second sheath liquid piping 42 can be turned on and off independently of each other, and the sheath liquid is supplied to the sheath liquid receiver 12 via the first sheath liquid piping 41 and/or the second sheath liquid piping 42 in the on state.

In this embodiment, a two-way valve may be disposed on each of the first sheath fluid line 41 and the second sheath fluid line 42, and the two-way valve on the first sheath fluid line 41 is opened and closed independently of the two-way valve on the second sheath fluid line 42. So that the primary sheath fluid piping 41 and the secondary sheath fluid piping 42 can be turned on and off independently of each other. And under the condition that the pushing pressure of sheath liquid is required to be smaller, the second sheath liquid pipeline 42 with larger pipe resistance can be conducted, the first sheath liquid pipeline 41 with smaller pipe resistance is cut off, and the sheath liquid is pushed through the second sheath liquid pipeline 42 by the first sheath liquid pushing gear with smaller pushing pressure.

Under the condition that the pushing pressure of sheath fluid is required to be large, the first sheath fluid pipeline 41 is conducted, the second sheath fluid pipeline 42 is cut off, and the sheath fluid is pushed through the first sheath fluid pipeline 41 by the second sheath fluid pushing gear with large pushing pressure; alternatively, the first sheath liquid pipeline 41 and the second sheath liquid pipeline 42 are both communicated, and the sheath liquid is pushed by the first sheath liquid pipeline 41 and the second sheath liquid pipeline 42 in a first sheath liquid thrust gear; or, the sheath liquid is pushed by the second sheath liquid pushing force gear through the first sheath liquid pipeline 41 and the second sheath liquid pipeline 42, so that under the condition of larger pushing pressure requirement, different pushing pressure can be selected, the pushing pressure of the sheath liquid supply unit 4 can be changed into multiple gears, and when the gears are switched, liquid flow is smoother, and turbulence is not easy to occur due to the gears or pressure mutation.

Further, the propellant liquid supply unit 5 includes a first propellant liquid line 51 and a second propellant liquid line 52 connected in parallel with each other.

The pipe diameter of the first propellant line 51 is larger than the pipe diameter of the second propellant line 52, so that the flow rate of the propellant in the first propellant line 51 is larger than the flow rate in the second propellant line 52 when the propellant is pushed in the same propellant thrust gear.

And/or the length of the first propellant line 51 is smaller than the length of the second propellant line 52. So that the flow rate of the propellant in the first propellant line 51 is greater than the flow rate in the second propellant line 52 when the propellant is propelled by the same propellant thrust gear.

It will be appreciated that in order to achieve a propulsion of the propulsion fluid in the same propulsion fluid thrust gear, the flow rate of the propulsion fluid in the first propulsion fluid line 51 is greater than the flow rate in the second propulsion fluid line 52. The pipe diameter of the first propellant line 51 in the blood cell analysis apparatus a is larger than the pipe diameter of the second propellant line 52, or the pipe length of the first propellant line 51 is smaller than the pipe length of the second propellant line 52, or the pipe diameter of the first propellant line 51 is larger than the pipe diameter of the second propellant line 52, and the pipe length of the first propellant line 51 is smaller than the pipe length of the second propellant line 52.

Wherein the first propellant line 51 and the second propellant line 52 can be switched on and off independently of each other, the propellant being fed into the sample receiving member 11 only in the on state of the first propellant line 51 and/or the second propellant line 52.

In the present embodiment, the first propellant line 51 and the second propellant line 52 may each be provided with one two-way valve, and the opening and closing of the two-way valve on the first propellant line 51 is independent of the opening and closing of the two-way valve on the second propellant line 52, so that the first propellant line 51 and the second propellant line 52 can be turned on and off independently of each other. And under the condition that the propulsion pressure is required to be smaller, the second propulsion liquid pipeline 52 with larger pipe resistance is conducted, the first propulsion liquid pipeline 51 with smaller pipe resistance is cut off, and the propulsion liquid is propelled by the first propulsion liquid pipeline 52 with smaller propulsion pressure in a thrust gear.

When the propulsion pressure is required to be high, the first propulsion liquid pipeline 51 is conducted, the second propulsion liquid pipeline 52 is cut off, and the propulsion liquid is propelled through the first propulsion liquid pipeline 51 by the second propulsion liquid thrust gear with the high propulsion pressure; alternatively, the first propellant line 51 and the second propellant line 52 are both connected, and the propellant is pushed through the first propellant line 51 and the second propellant line 52 in the first propellant thrust gear, or the propellant is pushed through the first propellant line 51 and the second propellant line 52 in the second propellant thrust gear. Under the condition of the requirement of large pushing pressure, the selection of different pushing pressure is still realized, the multi-shift of the pushing pressure of the pushing liquid supply unit 5 is realized, and when the shift is switched, the liquid flow is smoother, and turbulence is not easy to occur due to the shift or the abrupt change of pressure.

In summary, the sheath fluid supply unit 4 has at least two sheath fluid thrust steps with different pushing pressures, and has a first sheath fluid pipeline 41 and a second sheath fluid pipeline 42 which can be independently turned on and off and have different pipe resistances of the pipelines, the same sheath fluid thrust step is matched with different sheath fluid pipelines, the same sheath fluid pipeline is matched with different sheath fluid thrust steps, a plurality of different pushing pressures are used for pushing the sheath fluid, multiple steps of the sheath fluid supply unit 4 are realized, and when the steps are switched, fluid flow is smoother, and turbulence is not easy to occur due to steps or pressure mutation. The propulsion liquid supply unit 5 also has at least two propulsion liquid thrust gears with different propulsion pressures, and also has a first propulsion liquid pipeline 51 and a second propulsion liquid pipeline 52 which can be independently connected and disconnected and have different pipe resistances of the pipelines, the same propulsion liquid thrust gear is matched with different propulsion liquid pipelines, the same propulsion liquid pipeline is matched with different propulsion liquid thrust gears, a plurality of different propulsion pressures are used for propelling the propulsion liquid, the multi-gear of the propulsion liquid supply unit 5 is realized, and when the gears are switched, liquid flow is smoother, and turbulence is not easy to occur due to gear or pressure mutation.

The sheath liquid supply unit 4 and the propulsion liquid supply unit 5 are different in part model and parameter, so that the propulsion pressure provided by the sheath liquid supply unit 4 and the propulsion pressure provided by the propulsion liquid supply unit 5 are matched more, more propulsion pressure matched choices are provided for the blood cell analysis equipment A, the requirements of more detection channels on the propulsion pressure of sample liquid and sheath liquid can be met, the practicability and detection efficiency of the blood cell analysis equipment A are improved, and the use experience of a user on the blood cell analysis equipment A provided by the application is improved.

The foregoing description is only of embodiments of the present application, and is not intended to limit the scope of the patent application, and all equivalent structures or equivalent processes using the descriptions and the contents of the present application or other related technical fields are included in the scope of the patent application.

Claims

1. A blood cell analysis apparatus, characterized in that the blood cell analysis apparatus comprises:

A main line, the sample receiving member being connected to the main line;

2. The blood cell analysis apparatus of claim 1, further comprising a controller for:

3. The blood cell analysis apparatus according to claim 2, wherein the propellant liquid supply unit includes a first propellant liquid line and a second propellant liquid line, the first propellant liquid line and the second propellant liquid line being connected to the main line, respectively;

the controller is used for:

and/or, the controller is further configured to:

4. The blood cell analysis apparatus of claim 1, further comprising a controller for:

Controlling the sheath fluid supply unit to supply the sheath fluid to the sheath fluid receiving member at the propulsion pressure of the second sheath fluid thrust gear in the at least two sheath fluid thrust gears;

the propulsion pressure of the first sheath fluid thrust gear is smaller than that of the second sheath fluid thrust gear.

5. The blood cell analysis apparatus according to claim 4, wherein,

the sheath liquid supply unit comprises a first sheath liquid pipeline and a second sheath liquid pipeline, and the first sheath liquid pipeline and the second sheath liquid pipeline are respectively connected with the sheath liquid receiving piece;

the controller is further configured to:

6. The blood cell analysis apparatus according to claim 1, wherein,

the model of the components composing the sheath liquid supply unit is different from the model of the components at least partially corresponding to the model of the components composing the propulsion liquid supply unit;

7. The blood cell analysis apparatus according to claim 5, wherein,

the propellant liquid supply unit comprises a first propellant liquid pipeline and a second propellant liquid pipeline;

and/or the pipe diameter of the second sheath liquid pipeline is different from the pipe diameter of the second propellant liquid pipeline.

8. The blood cell analysis apparatus according to claim 5, wherein,

the propulsion liquid supply unit comprises a first propulsion liquid pipeline, a second propulsion liquid pipeline and a sample propulsion unit, when a sample is detected, the first propulsion liquid pipeline and the second propulsion liquid pipeline are closed, valves between the main pipeline and all the sample reaction units are closed, and the sample propulsion unit is used for pushing propulsion liquid to the main pipeline.

9. The blood cell analysis apparatus according to claim 8, wherein,

the sheath fluid supply unit is configured to supply the sheath fluid to the sheath fluid receiver at a pushing pressure of the first sheath fluid pushing force range when a valve between the main pipe and the sample reaction unit is closed,

Wherein,,

10. The blood cell analysis apparatus according to claim 1, wherein,

the sheath liquid supply unit comprises a first sheath liquid pipeline and a second sheath liquid pipeline which are connected in parallel;