CN114113571A - Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system - Google Patents
Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system Download PDFInfo
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- 239000007788 liquid Substances 0.000 title claims abstract description 130
- 238000004140 cleaning Methods 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000003018 immunoassay Methods 0.000 title claims abstract description 14
- 239000004005 microsphere Substances 0.000 claims abstract description 12
- 239000012530 fluid Substances 0.000 claims description 65
- 230000007246 mechanism Effects 0.000 claims description 41
- 238000000605 extraction Methods 0.000 claims description 24
- 239000002699 waste material Substances 0.000 claims description 24
- 238000011084 recovery Methods 0.000 claims description 21
- 238000005259 measurement Methods 0.000 claims description 17
- 238000001514 detection method Methods 0.000 claims description 14
- 230000003287 optical effect Effects 0.000 claims description 6
- 238000007599 discharging Methods 0.000 claims description 5
- 238000004891 communication Methods 0.000 claims 1
- 230000014759 maintenance of location Effects 0.000 claims 1
- 239000000523 sample Substances 0.000 description 40
- 238000005406 washing Methods 0.000 description 12
- 238000002156 mixing Methods 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000011109 contamination Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
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- 238000011534 incubation Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
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Abstract
The application relates to the technical field of medical equipment, and particularly discloses an immunoassay analyzer, a liquid path system thereof and a cleaning method of the liquid path system, wherein the liquid path system comprises: a flow chamber. And the cleaning liquid storage tank is communicated with the flow chamber. And the sheath liquid injector is respectively communicated with the cleaning liquid storage tank and the flow chamber, and is used for conveying the cleaning liquid in the cleaning liquid storage tank to the flow chamber, wherein the cleaning liquid is used for cleaning the inner wall of the flow chamber so as to remove microspheres in the flow chamber. Through the mode, the residual fluorescent coding microspheres in the flow chamber can be effectively removed, the manual cleaning operation of a user is not needed, and the risk that a sample to be detected is polluted is reduced.
Description
Technical Field
The application relates to the technical field of medical equipment, in particular to an immunoassay analyzer, a liquid path system of the immunoassay analyzer and a cleaning method of the liquid path system.
Background
The flow type fluorescence is also called as a suspension array, a liquid phase chip and the like, the technology takes a fluorescence coding microsphere as a core, integrates a plurality of technologies such as a current-collecting principle, laser analysis, high-speed digital signal processing and the like, performs multi-index parallel analysis, and has the characteristics of high flux, high sensitivity, parallel detection and the like; can be used for various and multi-field researches such as immunoassay, nucleic acid research, enzymology analysis, receptor, ligand recognition analysis and the like.
In the long-term research process, the inventor of the present application finds that if the detection speed is desired to be increased, the detection period is generally shortened, which also results in the shortened time for cleaning, and the fluorescent-coded microspheres are easy to remain in the flow chamber, increasing the risk of contamination of the sample to be detected.
Disclosure of Invention
In order to solve the technical problems, the application provides an immunoassay analyzer, a liquid path system thereof and a cleaning method of the liquid path system, which can clean and maintain a flow chamber, effectively remove residual fluorescent coding microspheres in the flow chamber and reduce the risk of contamination of a sample to be detected.
In order to solve the technical problem, the application adopts a technical scheme that: there is provided a fluid path system, comprising: a flow chamber. And the cleaning liquid storage tank is communicated with the flow chamber. And the sheath liquid injector is respectively communicated with the cleaning liquid storage tank and the flow chamber, and is used for conveying the cleaning liquid in the cleaning liquid storage tank to the flow chamber, wherein the cleaning liquid is used for cleaning the inner wall of the flow chamber so as to remove microspheres in the flow chamber.
In order to solve the above technical problem, another technical solution adopted by the present application is: an immunoassay analyzer is provided, which comprises the liquid path system and a control circuit.
In order to solve the above technical problem, the present application adopts another technical solution: the cleaning method of the liquid path system is provided, and the method is based on the liquid path system, and the cleaning method comprises the following steps: the sheath fluid injector is used for conveying the cleaning fluid in the cleaning fluid storage tank to the flow chamber to clean the flow chamber.
The beneficial effect of this application is: be different from prior art's condition, this application is carried the washing liquid in with the washing liquid holding vessel for flowing the room through sheath liquid syringe, and wherein, the washing liquid in the washing liquid holding vessel is used for wasing the inner wall that flows the room, effectively clears away remaining fluorescence coding microballon in the room that flows, need not the manual cleaning operation of user, reduces the contaminated risk of sample that awaits measuring.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts. Wherein:
FIG. 1 is a schematic diagram of a fluid circuit system according to an embodiment of the present application;
FIG. 2 is a schematic structural diagram of another embodiment of a fluid path system of the present application;
FIG. 3 is a schematic structural diagram of an embodiment of the immunoassay analyzer of the present application.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
In the present application, the reagent used in the immunoassay analyzer includes one or more of fluorescence-encoded microspheres, antibodies and fluorescent biotin, the original sample is a sample provided by the object to be tested, including but not limited to whole blood, serum, etc., and the sample to be tested is a sandwich structure including fluorescence-encoded microspheres-antigen-antibodies-fluorescent biotin. Sample particles to be detected flow through the flow chamber in an arrangement mode, particles in the sample flow are irradiated by laser, various types of scattered light signals generated in irradiation are collected, and the signals are classified and counted by combining biological characteristics to obtain parameter information with clinical significance. If the cleaning time is not enough, the fluorescent coding microspheres are easy to remain in the flow chamber, and the risk of contamination of the sample to be detected is increased.
In order to solve the technical problem, the application provides a liquid path system.
Referring to fig. 1, a fluid path system 10 according to an embodiment of the present disclosure includes: a flow chamber 11, a cleaning liquid storage tank 12, and a sheath liquid injector 13. The cleaning liquid storage tank 12 communicates with the flow chamber 11. The sheath liquid injector 13 is respectively communicated with the cleaning liquid storage tank 12 and the flow chamber 11, and the sheath liquid injector 13 is used for conveying the cleaning liquid in the cleaning liquid storage tank 12 to the flow chamber 11, wherein the cleaning liquid is used for cleaning the inner wall of the flow chamber 11 to remove microspheres (not shown) in the flow chamber 11.
The cleaning liquid storage tank 12 is used to supply new cleaning liquid to the flow chamber 11. That is, after the waste liquid in the flow chamber 11 is discharged, new cleaning liquid can be further refilled into the flow chamber 11 by using the cleaning liquid storage tank 12 to perform the secondary cleaning of the flow chamber 11.
Be different from prior art's condition, this application is carried the washing liquid in with washing liquid storage jar 12 for flowing room 11 through sheath liquid syringe 13, and wherein, the washing liquid in washing liquid storage jar 12 is used for wasing the inner wall that flows room 11 to clear away the microballon in flowing room 11, need not the user and manually carry out the cleaning operation, and effectively get rid of remaining fluorescence coding microballon in flowing room 11, reduce the contaminated risk of sample that awaits measuring.
Referring to fig. 2, in an embodiment, the fluid path system 10 further includes: a first solenoid valve LV11 and a second solenoid valve LV06, wherein a first port of the first solenoid valve LV11 is communicated with the sheath fluid injector 13, a second port of the first solenoid valve LV11 is communicated with a first port of the second solenoid valve LV06, a second port of the second solenoid valve LV06 is communicated with the cleaning fluid storage tank 12, and a third port of the second solenoid valve LV06 is communicated with the first port of the flow chamber 11.
The sheath liquid injector 13 is configured to deliver the cleaning liquid in the cleaning liquid storage tank 12 to the pipeline between the first solenoid valve LV11 and the second solenoid valve LV06 when the first port of the second solenoid valve LV06 is communicated with the second port of the second solenoid valve LV06, and the first port of the first solenoid valve LV11 is communicated with the second port of the first solenoid valve LV 11.
The sheath fluid injector 13 is further configured to deliver the cleaning fluid in the pipe between the first solenoid valve LV11 and the second solenoid valve LV06 to the flow chamber 11 to clean the flow chamber 11 when the third port of the second solenoid valve LV06 and the first port of the second solenoid valve LV06 are communicated with the first port of the first solenoid valve LV11 and the second port of the first solenoid valve LV11 is communicated with the second port of the first solenoid valve LV 11.
Referring to fig. 2, in an embodiment, the fluid path system 10 further includes: the device comprises a suction pump 14, a buffer mechanism 15, a waste liquid recovery tank 16, a third electromagnetic valve LV07 and an eighth electromagnetic valve LV10, wherein a first interface of the third electromagnetic valve LV07 is communicated with a second interface of the flow chamber 11, a second interface of the third electromagnetic valve LV07 is communicated with a first interface of the buffer mechanism 15, a first interface of the eighth electromagnetic valve LV10 is communicated with a second interface of the buffer mechanism 15, a second interface of the eighth electromagnetic valve LV10 is communicated with the suction pump 14, and the suction pump 14 is communicated with the waste liquid recovery tank 16.
The extraction pump 14 is configured to discharge the liquid in the flow chamber 11 to the waste liquid recovery tank 16 when the first port of the third solenoid valve LV07 is communicated with the second port of the third solenoid valve LV07, the first port of the eighth solenoid valve LV10 is communicated with the second port of the eighth solenoid valve LV10, and the extraction pump 14 is opened.
Referring to fig. 2, in an embodiment, the fluid path system 10 further includes: the sheath fluid storage tank 17, the buffer mechanism 15, a third solenoid valve LV07, a fourth solenoid valve LV05, a fifth solenoid valve LV02 and a sixth solenoid valve LV04, wherein a first port of the third solenoid valve LV07 is communicated with a second port of the flow chamber 11, a second port of the third solenoid valve LV07 is communicated with a first port of the buffer mechanism 15, a first port of the fourth solenoid valve LV05 is communicated with the sheath fluid injector 13, a second port of the fourth solenoid valve LV05 is communicated with a first port of the first solenoid valve LV11, a third port of the fourth solenoid valve LV05 is communicated with a first port of the fifth solenoid valve LV02 and a first port of the sixth solenoid valve LV04, a second port of the fifth solenoid valve LV02 is communicated with a third port of the flow chamber 11, and a second port of the sixth solenoid valve LV04 is communicated with the sample injector 19.
The sheath fluid injector 13 is further configured to deliver the sheath fluid in the sheath fluid storage tank 17 to a conduit between the fourth solenoid valve LV05 and the first LV solenoid valve 11 when the first port of the sixth solenoid valve LV04 is not communicated with the second port of the sixth solenoid valve LV04, the first port of the fifth solenoid valve LV02 is not communicated with the second port of the fifth solenoid valve LV02, the first port of the third solenoid valve LV07 is not communicated with the second port of the third solenoid valve LV07, the first port of the fourth solenoid valve LV05 is communicated with the second port of the fourth solenoid valve LV05, and the first port of the first solenoid valve LV11 is communicated with the third port of the first solenoid valve LV 11.
The sheath fluid injector 13 is further configured to deliver the sheath fluid in the conduit between the fourth solenoid valve LV05 and the first solenoid valve LV11 to the flow chamber 11 when the first port of the sixth solenoid valve LV04 is communicated with the second port of the sixth solenoid valve LV04, the first port of the fifth solenoid valve LV02 is communicated with the second port of the fifth solenoid valve LV02, the first port of the third solenoid valve LV07 is communicated with the second port of the third solenoid valve LV07, and the first port of the fourth solenoid valve LV05 is communicated with the third port of the fourth solenoid valve LV 05.
In this embodiment, the sheath liquid and the cleaning liquid may be the same or different in composition.
Referring to fig. 2, in an embodiment, the fluid path system 10 further includes: a sample injector 19, a measurement needle mechanism 21 and a seventh solenoid valve LV 01.
The measurement probe mechanism 21 includes a measurement probe (not shown) and a stirring member (not shown), and the measurement probe mechanism 21 is used for sucking a sample to be detected into the optical detection mechanism for detection; the stirring piece stirs the mixture of the original sample and the fluorescent coding microspheres, so that the antigen in the original sample is fully combined with the specific antibody in the fluorescent coding microspheres.
And preparing a sample to be detected after the incubation and the magnetic separation are finished. Thereafter, the sample to be measured is sucked into the optical detection mechanism by the measurement probe mechanism 21 and detected. In addition, before the sample to be detected is sucked into the optical detection mechanism for detection, the method also comprises the step of uniformly mixing the sample to be detected, wherein the uniformly mixing method comprises one or more of mechanical uniform mixing, bubble uniform mixing or sucking and spitting uniform mixing. In other implementations; in the example, the measurement tip mechanism 21 mixes the sample in the cuvette (not shown) by sucking and discharging the sample, and thus, a separate stirring device is not required, which is advantageous for simplifying the structure of the immunoassay analyzer.
The first port of the sample injector 19 is communicated with the second port of the sixth solenoid valve LV04, the second port of the sample injector 19 is communicated with the fourth port of the flow chamber 11 and the first port of the seventh solenoid valve LV01, respectively, and the second port of the seventh solenoid valve LV01 is communicated with the first port of the measurement needle mechanism 21.
After the samples to be measured are uniformly mixed, the measuring needle mechanism 21 moves to the bottom of the reaction cup, at this time, the sheath fluid injector 13 is used for conveying the samples to be measured to a pipeline between the seventh electromagnetic valve LV01 and the sample injector 19 when the first interface of the seventh electromagnetic valve LV01 is communicated with the second interface of the seventh electromagnetic valve LV01, the first interface of the sixth electromagnetic valve LV04 is communicated with the second interface of the sixth electromagnetic valve LV04, and the first interface of the fourth electromagnetic valve LV05 is communicated with the third interface of the fourth electromagnetic valve LV05, and it needs to be noted that at this time, the liquid level of the samples to be measured is just at the first interface of the seventh electromagnetic valve LV 01.
The sheath fluid injector 13 is configured to deliver the sample to be tested in the pipeline between the seventh electromagnetic valve LV01 and the sample injector 19 to the flow chamber 11 when the first interface of the seventh electromagnetic valve LV01 is not communicated with the second interface of the seventh electromagnetic valve LV 01.
Further, the piston of the sheath fluid injector 13 and the piston of the sample injector 19 are driven by the same driving mechanism for applying a pushing force or a pulling force to the piston of the sheath fluid injector 13 and the piston of the sample injector 19. The capacity of the sheath liquid injector 13 is smaller than that of the sample injector 19, in the embodiment, the sheath liquid injector 13 and the sample injector 19 enable the pressure in the pipeline to have different change amplitudes, the sample injector 19 performs coarse adjustment on the pressure in the pipeline in order of magnitude, the sheath liquid injector 13 performs fine adjustment on the pressure in the pipeline in specific numerical value, and the combination of the large-volume injector and the small-volume injector can quickly and accurately control the pressure in the pipeline and the volume of the liquid conveyed in the pipeline, so that the operation efficiency is improved.
Referring to fig. 2, in an embodiment, the fluid path system 10 further includes: the device comprises an extraction pump 14, a waste liquid recovery tank 16 and an eighth electromagnetic valve LV10, wherein a first port of the eighth electromagnetic valve LV10 is communicated with a second port of a buffer mechanism 15, a second port of the eighth electromagnetic valve LV10 is communicated with the extraction pump 14, and the extraction pump 14 is communicated with the waste liquid recovery tank 16.
The extraction pump 14 is configured to discharge the liquid in the flow chamber 11 to the waste liquid recovery tank 16 when the first port of the third solenoid valve LV07 is communicated with the second port of the third solenoid valve LV07, the first port of the eighth solenoid valve LV10 is communicated with the second port of the eighth solenoid valve LV10, and the extraction pump 14 is opened.
Further, the sheath liquid storage tank 17 and the cleaning liquid storage tank 12 are respectively provided with a liquid level detection device (not shown), and when the liquid levels of the sheath liquid storage tank 17 and the cleaning liquid storage tank 12 are detected to be lower than preset values, an alarm is given to supplement the sheath liquid and the cleaning liquid. Of course, the waste liquid recovery tank 16 is also provided with a liquid level detection device for alarming and reminding the waste liquid cleaning when the liquid level of the waste liquid recovery tank 16 exceeds the preset height.
Specifically, the waste liquid recovery tank 16 is provided at an end of a drain line (not shown) through which the suction pump 14 is connected. Therefore, the waste liquid discharged from the liquid path system 10 can be injected into the waste liquid recovery tank 16 through the relevant path. The extraction pump 14 can be a diaphragm pump, which, in contrast to peristaltic pumps, does not require frequent replacement of the relative tubing, having a longer life and at the same time facilitating better maintenance.
Referring to fig. 2, in an embodiment, the fluid path system 10 further includes: a ninth electromagnetic valve LV08, a first port of the ninth electromagnetic valve LV08 is communicated with a second port of the measuring needle mechanism 21, a second port of the ninth electromagnetic valve LV08 is communicated with the extraction pump 14, and the extraction pump 14 is communicated with the waste liquid recovery tank 16.
The sheath liquid injector 13 is configured to deliver the sheath liquid in the sheath liquid injector 13 to the measurement needle mechanism 21 and discharge the sheath liquid in the measurement needle mechanism 21 to the waste liquid recovery tank 16 when the first port of the fourth solenoid valve LV05 is communicated with the third port of the fourth solenoid valve LV05, the first port of the sixth solenoid valve LV04 is communicated with the second port of the sixth solenoid valve LV04, the first port of the seventh solenoid valve LV01 is communicated with the second port of the seventh solenoid valve LV01, and the first port of the ninth solenoid valve LV08 is communicated with the second port of the ninth solenoid valve LV08, and the extraction pump 14 is turned on.
Referring to fig. 2, in an embodiment, the sheath fluid injector 13 is configured to convey the sheath fluid in the sheath fluid injector to the lower port of the swab of the measurement needle mechanism and to drain the sheath fluid through the upper port of the swab of the measurement needle mechanism when the first port of the fourth solenoid valve LV05 is communicated with the second port of the fourth solenoid valve LV05, the first port of the ninth solenoid valve LV08 is communicated with the second port of the ninth solenoid valve LV08, and the draw pump 14 is turned on.
Referring to fig. 2, in an embodiment, the fluid path system 10 further includes: a tenth solenoid valve LV09, the first port of the tenth solenoid valve LV09 is communicated with the third port of the measurement needle mechanism 21, and the second port of the tenth solenoid valve LV09 is communicated with the first port of the sixth solenoid valve LV04 and the third port of the fourth solenoid valve LV05, respectively.
The sheath fluid injector 13 is configured to deliver the sheath fluid in the sheath fluid injector 13 to the measurement needle mechanism 21 when the first port of the fourth solenoid valve LV05 is communicated with the third port of the fourth solenoid valve LV05, the first port of the sixth solenoid valve LV04 is not communicated with the second port of the sixth solenoid valve LV04, and the first port of the tenth solenoid valve LV09 is communicated with the second port of the tenth solenoid valve LV 09.
Referring to fig. 3, the present application further provides an immunoassay analyzer 100, wherein the immunoassay analyzer 100 includes the liquid path system 10, the optical detection device 20, and the control circuit 30 according to the above-mentioned embodiment. In this embodiment, the optical detection device 20 includes a laser emitting device (not shown) and a light beam collecting device (not shown), the sample particles to be detected flow through the flow chamber in an aligned manner, the laser emitting device emits laser light on the sample particles to be detected wrapped by the sheath fluid, and the light beam collecting device collects the intensity of scattered light and fluorescence emitted by the sample particles to be detected under the irradiation of the laser light, so as to further determine the classification and quantity of the sample to be detected.
The application also provides a cleaning method of the liquid path system, which is based on the liquid path system of the embodiment, and the cleaning method comprises the following steps: and delivering the cleaning solution in the cleaning solution storage tank to the flow chamber through the sheath solution injector to clean the flow chamber, wherein the residence time of the cleaning solution in the flow chamber is 3-15 minutes, such as 3 minutes, 5 minutes, 10 minutes or 15 minutes.
Be different from prior art's condition, this application is carried the washing liquid in with the washing liquid holding vessel for flowing the room through sheath liquid syringe, and wherein, the washing liquid in the washing liquid holding vessel is used for wasing the inner wall that flows the room, effectively clears away remaining fluorescence coding microballon in the room that flows, need not the manual cleaning operation of user, reduces the contaminated risk of sample that awaits measuring.
The above description is only for the purpose of illustrating embodiments of the present application and is not intended to limit the scope of the present application, and all modifications of equivalent structures and equivalent processes, which are made by the contents of the specification and the drawings of the present application or are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.
Claims (12)
1. A fluid path system, comprising:
a flow chamber;
a cleaning fluid storage tank in communication with the flow chamber; and
and the sheath liquid injector is respectively communicated with the cleaning liquid storage tank and the flow chamber, and is used for conveying the cleaning liquid in the cleaning liquid storage tank to the flow chamber, wherein the cleaning liquid is used for cleaning the inner wall of the flow chamber so as to remove the microspheres in the flow chamber.
2. The fluid path system of claim 1, further comprising: a first solenoid valve and a second solenoid valve, wherein a first port of the first solenoid valve is communicated with the sheath fluid injector, a second port of the first solenoid valve is communicated with a first port of the second solenoid valve, a second port of the second solenoid valve is communicated with the cleaning fluid storage tank, and a third port of the second solenoid valve is communicated with the first port of the flow chamber;
the sheath fluid injector is used for conveying the cleaning fluid in the cleaning fluid storage tank to a pipeline between the first electromagnetic valve and the second electromagnetic valve when the first interface of the second electromagnetic valve is communicated with the second interface of the second electromagnetic valve and the first interface of the first electromagnetic valve is communicated with the second interface of the first electromagnetic valve;
the sheath fluid injector is further configured to deliver the cleaning fluid in the pipe between the first electromagnetic valve and the second electromagnetic valve to the flow chamber when the third interface of the second electromagnetic valve is communicated with the first interface of the second electromagnetic valve and the first interface of the first electromagnetic valve is communicated with the second interface of the first electromagnetic valve.
3. The fluid path system of claim 2, further comprising: the waste liquid recovery device comprises an extraction pump, a buffer mechanism, a waste liquid recovery tank, a third electromagnetic valve and an eighth electromagnetic valve, wherein a first interface of the third electromagnetic valve is communicated with a second interface of the flow chamber, a second interface of the third electromagnetic valve is communicated with a first interface of the buffer mechanism, a first interface of the eighth electromagnetic valve is communicated with a second interface of the buffer mechanism, a second interface of the eighth electromagnetic valve is communicated with the extraction pump, and the extraction pump is communicated with the waste liquid recovery tank;
and the extraction pump is used for discharging the liquid in the flowing chamber to the waste liquid recovery tank when the first interface of the third electromagnetic valve is communicated with the second interface of the third electromagnetic valve and the first interface of the eighth electromagnetic valve is communicated with the second interface of the eighth electromagnetic valve.
4. The fluid path system of claim 2, further comprising: the sheath fluid storage tank is communicated with a second interface of the flow chamber, a second interface of the third electromagnetic valve is communicated with a first interface of the buffer mechanism, a first interface of the fourth electromagnetic valve is communicated with the sheath fluid injector, a second interface of the fourth electromagnetic valve is communicated with a first interface of the first electromagnetic valve, a third interface of the fourth electromagnetic valve is respectively communicated with a first interface of the fifth electromagnetic valve and a first interface of the sixth electromagnetic valve, a second interface of the fifth electromagnetic valve is communicated with a third interface of the flow chamber, and a second interface of the sixth electromagnetic valve is communicated with the sample injector;
the sheath liquid injector is further configured to convey the sheath liquid in the sheath liquid storage tank to a pipeline between the fourth electromagnetic valve and the first electromagnetic valve when the first interface of the sixth electromagnetic valve is not communicated with the second interface of the sixth electromagnetic valve, the first interface of the fifth electromagnetic valve is not communicated with the second interface of the fifth electromagnetic valve, the first interface of the third electromagnetic valve is not communicated with the second interface of the third electromagnetic valve, the first interface of the fourth electromagnetic valve is communicated with the second interface of the fourth electromagnetic valve, and the first interface of the first electromagnetic valve is communicated with the third interface of the first electromagnetic valve;
the sheath liquid injector is further configured to convey the sheath liquid in the pipeline between the fourth electromagnetic valve and the first electromagnetic valve to the flow chamber when the first interface of the sixth electromagnetic valve is communicated with the second interface of the sixth electromagnetic valve, the first interface of the fifth electromagnetic valve is communicated with the second interface of the fifth electromagnetic valve, the first interface of the third electromagnetic valve is communicated with the second interface of the third electromagnetic valve, and the first interface of the fourth electromagnetic valve is communicated with the third interface of the fourth electromagnetic valve.
5. The fluid path system of claim 4, further comprising: the sample injector, the measuring needle mechanism and the seventh electromagnetic valve;
the first interface of the sample injector is communicated with the second interface of the sixth electromagnetic valve, the second interface of the sample injector is respectively communicated with the fourth interface of the flow chamber and the first interface of the seventh electromagnetic valve, and the second interface of the seventh electromagnetic valve is communicated with the first interface of the measurement needle mechanism;
the measuring needle mechanism is used for sucking a sample to be detected into the optical detection mechanism for detection;
the sheath fluid injector is used for conveying the sample to be detected to a pipeline between the seventh electromagnetic valve and the sample injector when the first interface of the seventh electromagnetic valve is communicated with the second interface of the seventh electromagnetic valve, the first interface of the sixth electromagnetic valve is communicated with the second interface of the sixth electromagnetic valve, and the first interface of the fourth electromagnetic valve is communicated with the third interface of the fourth electromagnetic valve;
the sheath fluid injector is used for conveying the sample to be detected in the pipeline between the seventh electromagnetic valve and the sample injector to the flow chamber when the first interface of the seventh electromagnetic valve is not communicated with the second interface of the seventh electromagnetic valve.
6. The fluid path system of any of claims 4-5, further comprising: the buffer mechanism comprises an extraction pump, a waste liquid recovery tank and an eighth electromagnetic valve, wherein a first interface of the eighth electromagnetic valve is communicated with a second interface of the buffer mechanism, a second interface of the eighth electromagnetic valve is communicated with the extraction pump, and the extraction pump is communicated with the waste liquid recovery tank;
and the extraction pump is used for discharging the liquid in the flow chamber to the waste liquid recovery tank when the first interface of the third electromagnetic valve is communicated with the second interface of the third electromagnetic valve, the first interface of the eighth electromagnetic valve is communicated with the second interface of the eighth electromagnetic valve, and the extraction pump is opened.
7. The fluid path system of claim 6, further comprising: a first port of the ninth electromagnetic valve is communicated with a second port of the measuring needle mechanism, a second port of the ninth electromagnetic valve is communicated with the extraction pump, and the extraction pump is communicated with the waste liquid recovery tank;
the sheath liquid injector is used for conveying the sheath liquid in the sheath liquid injector to the measurement needle mechanism and discharging the sheath liquid in the measurement needle mechanism to the waste liquid recovery tank when the first interface of the fourth electromagnetic valve is communicated with the third interface of the fourth electromagnetic valve, the first interface of the sixth electromagnetic valve is communicated with the second interface of the sixth electromagnetic valve, the first interface of the seventh electromagnetic valve is communicated with the second interface of the seventh electromagnetic valve, the first interface of the ninth electromagnetic valve is communicated with the second interface of the ninth electromagnetic valve, and the extraction pump is opened.
8. The fluid path system of claim 7,
and the sheath liquid injector is used for conveying the sheath liquid in the sheath liquid injector to the lower port of the swab of the measuring needle mechanism when the first interface of the fourth electromagnetic valve is communicated with the second interface of the fourth electromagnetic valve, the first interface of the ninth electromagnetic valve is communicated with the second interface of the ninth electromagnetic valve and the extraction pump is opened, and discharging the sheath liquid through the upper port of the swab of the measuring needle mechanism.
9. The fluid path system of claim 5,
the fluid path system further includes: a tenth electromagnetic valve, a first interface of which is communicated with a third interface of the measurement needle mechanism, and a second interface of which is respectively communicated with a first interface of the sixth electromagnetic valve and a third interface of the fourth electromagnetic valve;
the sheath fluid injector is used for conveying sheath fluid in the sheath fluid injector to the measuring needle mechanism when the first interface of the fourth electromagnetic valve is communicated with the third interface of the fourth electromagnetic valve, the first interface of the sixth electromagnetic valve is not communicated with the second interface of the sixth electromagnetic valve, and the first interface of the tenth electromagnetic valve is communicated with the second interface of the tenth electromagnetic valve.
10. An immunoassay analyzer comprising the fluid circuit system of any one of claims 1-9 and a control circuit.
11. A method for cleaning a fluid path system, the method being based on the fluid path system according to any one of claims 1 to 9, the method comprising:
delivering the cleaning solution in the cleaning solution storage tank to the flow chamber through the sheath solution injector to clean the flow chamber.
12. The method of claim 11,
the retention time of the cleaning liquid in the flow chamber is 3-15 minutes.
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