CN112485251A

CN112485251A - Specific protein analyzer and blending method

Info

Publication number: CN112485251A
Application number: CN201910860447.9A
Authority: CN
Inventors: 谢子贤; 郁琦; 刘海
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2019-09-11
Filing date: 2019-09-11
Publication date: 2021-03-12

Abstract

A specific protein analyzer comprising: a sample supply device; a reagent supply device; the mixing pool is used for receiving a blood sample to be detected and the latex reagent to form mixed sample liquid; the detection device comprises a detection area and a light source; the blending component comprises a blending channel and a blending power device, the blending channel comprises a first end and a second end and is filled with liquid, the first end is communicated with the inner space of the blending pool, and the second end is connected with the blending power device; the blending power device drives the liquid in the blending channel to flow in the direction far away from the blending pool, and the liquid in the blending pool is sucked out of the blending channel; the blending power device drives the liquid in the blending channel to flow towards the direction close to the blending pool, the liquid in the blending channel is pushed into the blending pool to form a rotational flow, and the above steps are repeated in a circulating manner to blend the mixed sample liquid; no bubble is generated in the process of blending operation, and the detection accuracy is high.

Description

Specific protein analyzer and blending method

Technical Field

The application relates to the technical field of medical equipment, in particular to a specific protein analyzer and a blending method applied to the specific protein analyzer.

Background

With the popularization of clinical application, more and more parameters need to be detected in the field of blood test. From the first blood routine three-class, five-class parameters to later Protein-specific parameters, such as the CRP (C-Reactive Protein) parameter. The detection of specific protein parameters is typically performed using transmission and/or nephelometry.

To detect the amount of a specific protein (antigen), it is necessary to add specific latex particles (antibodies) to the blood sample. The latex particles are nano-scale spherical particles, and can react and combine with specific surrounding protein under certain conditions to form micelles with larger volume. As the latex particles continue to bind to the specific protein, the micelles formed gradually increase in size. The content of the specific protein in the blood sample can be obtained by gradually increasing the scattering signal and gradually decreasing the transmission signal formed after the light irradiation with the specific wavelength, monitoring the change rate of the transmission and/or scattering signal and calculating to a certain extent. In the case of a test using a whole blood sample, it is also necessary to add a hemolytic agent to the blood sample to lyse blood cells in the blood sample before adding the specific latex particles.

Therefore, a latex reagent (a suspension containing latex particles) is required for detecting a specific protein. However, since the latex reagent has large fluid properties such as viscosity and density, it is difficult to mix the blood sample and the latex reagent uniformly, and the reaction is not sufficient due to non-uniform mixing, which finally affects the detection result.

Disclosure of Invention

In order to solve the technical problems, the present application mainly aims to provide a specific protein analyzer with a good blending effect and a blending method applied to the specific protein analyzer.

In order to achieve the above technical problem, an embodiment of the present application provides a specific protein analyzer, including:

the sample supply device is used for providing a blood sample to be tested;

the reagent supply device is used for providing latex reagents which react with the blood sample to be detected;

the blending pool is used for receiving the blood sample to be detected supplied by the sample supply device and the latex reagent supplied by the reagent supply device so as to enable the blood sample to be detected to react with the latex reagent to form a mixed sample liquid;

a detection device including a detection area made of a light-transmitting material and a light source disposed corresponding to the detection area, the light source being configured to irradiate the mixed sample liquid flowing through the detection area so as to detect a specific protein content in the mixed sample liquid;

a blending component which comprises a blending channel and a blending power device, wherein the blending channel comprises a first end and a second end and is filled with liquid, the first end is communicated with the inner space of the blending pool, the second end is connected with the blending power device,

the mixing power device is used for driving liquid in the mixing channel to flow in a direction far away from the mixing pool or flow in a direction close to the mixing pool, so that the liquid in the mixing pool is sucked out of the mixing channel or pushed into the mixing pool.

In one embodiment, the blending power device may be configured as an injector, a quantitative pump, or a positive/negative pressure source, and is configured to provide a negative pressure for driving the liquid in the blending channel to flow in a direction away from the blending pool or a positive pressure for driving the liquid in the blending channel to flow in a direction close to the blending pool.

In one embodiment, the blending power device may be connected to the second end of the blending passage through a first control valve to connect or disconnect the blending power device to the blending passage.

In one embodiment, the specific protein analyzer further comprises a liquid source, the liquid source is connected with the blending power device, and the liquid source is used for providing liquid for the blending power device, so that the liquid can be provided for the blending channel.

In one embodiment, the liquid source may be connected to the mixing power device through the first control valve, and the control valve includes a first port, a second port, and a third port;

the first interface is connected with the blending power device, the second interface is connected with the liquid source, and the third interface is connected with the blending channel;

the first control valve controls the on-off of the blending power device and the blending channel by controlling the on-off of the first interface and the third interface;

the first control valve controls the on-off of the liquid source and the blending power device by controlling the on-off of the first interface and the second interface.

In one embodiment, the fluid source may be connected to the blending power plant via a second control valve to connect or disconnect the blending power plant to the fluid source.

In one embodiment, the detection zone may be disposed on the mixing well.

In one embodiment, the detection area of the detection device may be a part of the mixing channel, and when performing the detection operation, the mixing power device sucks the mixed sample liquid in the mixing pool out of the mixing channel, and the light source can irradiate the mixed sample liquid flowing through the part of the mixing channel constituting the detection area, so as to detect the content of the specific protein in the mixed sample liquid.

In one embodiment, the detection area may be configured as a detection channel independent from the mixing channel, the detection channel being in communication with the inner space of the mixing well and being filled with liquid;

the specific protein analyzer can further comprise a detection driving device, and the detection driving device is connected with the detection channel; the detection driving device is used for providing negative pressure to drive the liquid in the detection channel to flow in the direction far away from the mixing pool, and the mixed sample liquid which is mixed in the mixing pool is sucked out of the detection channel to be detected by specific protein.

In one embodiment, the blending power device and the detection driving device may be the same driving device, and the blending power device may be connected to the detection channel through a third control valve, so as to connect or disconnect the blending power device and the detection channel.

In one embodiment, the specific protein analyzer may further include a waste liquid channel and a waste liquid driving device, wherein two ends of the waste liquid channel are respectively connected to the inner space of the mixing tank and the waste liquid driving device; and the waste liquid driving device is used for discharging the liquid in the mixing pool through the waste liquid channel.

In one embodiment, the sample supply device and the reagent supply device may be constituted as the same pipette needle, or may be constituted as a sample needle and a reagent needle independent of each other.

In one embodiment, the specific protein analyzer may further include a hemolytic agent supply device including a hemolytic agent supply channel; the hemolytic agent supply channel is communicated with the blending pool and is used for supplying hemolytic agent to the blending pool to react with the blood sample to be tested.

In one embodiment, the specific protein analyzer may further comprise a blood routine detection module for classifying and/or counting cells in the blood sample to be tested.

In one embodiment, the specific protein analyzer may further include a controller electrically connected to the sample supply device, the reagent supply device, and the mixing power device, respectively, and configured to control the sample supply device, the reagent supply device, and the mixing power device, respectively.

The embodiment of the present application further provides a blending method, which is applied to the specific protein analyzer according to any one of the above embodiments, and the blending method includes:

the sample supply device and the reagent supply device respectively add a blood sample to be detected and an emulsion reagent into the mixing pool to form mixed sample liquid;

the blending power device executes at least one time of the following suction-push blending operation,

driving the liquid in the blending channel to flow in a direction far away from the blending pool so as to suck the liquid in the blending pool out of the blending channel;

and then driving the liquid in the mixing channel to flow towards the direction close to the mixing pool so as to push the liquid in the mixing channel into the mixing pool, thereby forming a rotational flow in the mixing pool to uniformly mix the blood sample to be detected and the latex reagent.

In one embodiment, in each of the suction-push blending operations, the volume of the liquid sucked into the blending passage by the blending power device may be smaller than the volume of the liquid pushed into the blending tank by the blending power device.

In one embodiment, in each of the suction-push kneading operations, the volume of the liquid sucked into the kneading passage by the kneading power unit may be equal to or greater than one-fourth of the volume of the liquid in the kneading tank.

In one embodiment, the kneading power device may perform the suction-push kneading operation a plurality of times, and in each suction-push kneading operation, the volume of the liquid sucked into the kneading passage by the kneading power device is the same as or different from the volume of the liquid pushed into the kneading tank by the kneading power device.

In one embodiment, the blood sample to be tested may be a whole blood sample, and before the reagent supplying device adds the latex reagent into the mixing pool, the method further includes:

and adding a hemolytic agent into the mixing pool.

The beneficial effect of this application: the application provides a specific protein analyzer is through setting up mixing passageway and mixing power device, the first end of mixing passageway is connected with the mixing pond, the second end is connected with mixing power device, and be full of liquid in the mixing passageway, mixing power device drives the direction flow of liquid in the mixing passageway to keeping away from the mixing pond, with liquid suction in the mixing pond to the mixing passageway in, mixing power device drives the liquid in the mixing passageway again and flows to the direction that is close to the mixing pond, liquid in the mixing passageway is pushed into in the mixing pond, form the whirl in the mixing pond, so circulation is reciprocal, make the mixed sample liquid mixing in the mixing pond. Because the mixing channel is filled with liquid, the gas in the mixing channel is emptied, no bubble is generated in the mixing operation process, and the detection accuracy is high.

Drawings

The advantages of the above and/or additional aspects of the present application will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a flow chart of the assay for detecting a specific protein;

FIG. 2 is a schematic diagram of the structure of a particular protein analyzer in one embodiment of the present application;

FIG. 3 is a schematic illustration of a homogenizing assembly of a particular protein analyzer in one embodiment of the present application;

FIGS. 4-7 are schematic structural diagrams of different embodiments of specific protein analyzers provided herein, respectively;

FIG. 8 is a functional block diagram of a particular protein analyzer in one embodiment of the present application;

fig. 9 and 10 are flow diagrams of various embodiments of a blending method provided herein.

Wherein, the corresponding relations between the reference numbers and the part names in fig. 2 to 8 are as follows:

10. a sample supply device; 20. a reagent supply device; 30. a blending pool; 40. a detection device; 41. a detection zone; 42. a light source; 50. a blending component; 51. a blending channel; 511. a first end; 512. a second end; 52. a blending power device; 60. a source of liquid; 61. another source of liquid; 70. a first control valve; 71. a first interface; 72. a second interface; 73. a third interface; 74. a third control valve; 75. a fourth control valve; 80. a detection channel; 81. detecting a driving device; 90. a waste liquid channel; 100. a waste liquid driving device; 110. a hemolytic agent supply device; 111. a hemolytic agent supply channel; 112. a hemolytic agent driving device; 120. a blood routine detection module; 130. and a controller.

Detailed Description

In order that the above objects, features and advantages of the present invention can be more clearly understood, a more particular description of the invention will be rendered by reference to the appended drawings. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.

The application provides a specific protein analyzer for detecting the content of specific protein in a blood sample to be detected. The blood sample to be tested may be a whole blood sample containing blood cells, or may be serum or plasma containing no blood cells.

When the blood sample to be detected is a whole blood sample, a hemolytic agent needs to be added into the whole blood sample to react and dissolve blood cells, and then a latex reagent is added to detect the content of the specific protein.

If the blood sample to be tested is a whole blood sample, please refer to fig. 1, the testing process includes the following steps:

step S01, add the blood sample to be tested.

And step S02, adding a hemolytic agent, uniformly mixing, reacting and dissolving blood cells in the blood sample to be tested.

In step S03, a latex reagent is added.

And step S04, mixing the latex reagent with the blood sample to be detected with the blood cells dissolved away.

And step S05, detecting the uniformly mixed liquid by a transmission and/or scattering turbidimetry method, and calculating to obtain the content of the specific protein in the blood sample to be detected.

At step S06, the state is restored to prepare for testing the next blood sample to be tested.

When the blood sample to be measured is serum or plasma, the step S02 is not performed.

Referring to fig. 2, in one embodiment, the present application provides a specific protein analyzer comprising:

a sample supply device 10 for supplying a blood sample to be tested;

a reagent supplying device 20 for supplying a latex reagent that reacts with the blood sample to be measured;

a mixing tank 30 for receiving the blood sample to be tested supplied by the sample supply device 10 and the latex reagent supplied by the reagent supply device 20, so that the blood sample to be tested reacts with the latex reagent to form a mixed sample liquid;

a detection device 40 including a detection region 41 made of a light-transmitting material and a light source 42 disposed corresponding to the detection region 41, the light source 42 being configured to irradiate the mixed sample liquid flowing through the detection region 41 so as to detect the content of the specific protein in the mixed sample liquid;

the blending component 50 comprises a blending passage 51 and a blending power device 52, the blending passage 51 comprises a first end 511 and a second end 512 and is filled with liquid, the first end 511 is communicated with the inner space of the blending pool 30, the second end 512 is connected with the blending power device 52,

the blending power device 52 is used for driving the liquid in the blending passage 51 to flow in a direction far away from the blending pool 30 or flow in a direction close to the blending pool 30, so as to suck the liquid in the blending pool 30 out of the blending passage 51 or push the liquid in the blending passage 51 into the blending pool 30.

The working principle and process of the specific protein analyzer provided by the embodiment are as follows:

the blood sample to be measured and the latex reagent are added into the mixing cell 30 through the sample supply device 10 and the reagent supply device 20, respectively, to form a mixed sample solution. However, at this time, the blood sample to be measured and the latex reagent are not sufficiently mixed and are in an uneven state. The specific protein detection can be carried out only after the mixture is fully mixed, and then the mixing operation is required.

It should be noted that, when the specific protein analyzer is put into use, it is first necessary to fill the liquid path components in the specific protein analyzer with liquid so as to exhaust the gas in the liquid path, thereby ensuring the accuracy of the detection result. Thus, when using a particular protein analyzer, the mixing channel 51 is also liquid-filled. And the first end 511 of the mixing channel 51 has a smaller diameter, so that the liquid in the mixing channel 51 cannot overflow into the mixing pool 30. In other embodiments, the height of the first end 511 relative to the second end 512 may be increased to prevent the liquid in the mixing channel 51 from flowing into the mixing tank 30 due to gravity. The mixing passage 51 is filled with a diluent.

When the blending operation is performed, the blending power device 52 first drives the liquid in the blending passage 51 to move in a direction away from the blending pool 30, so as to suck a part of the liquid in the blending pool 30 out of the blending passage 51. The blending power device 52 drives the liquid in the blending passage 51 to move towards the blending pool 30, so as to push a part of the liquid in the blending passage 51 into the blending pool 30, and form a rotational flow in the blending pool 30. The circulation is repeated, so that the blood sample to be tested and the latex reagent in the mixing pool 30 are mixed uniformly. Because the blending channel 51 is filled with liquid in the initial state, the gas in the blending channel 51 is emptied, no bubble is generated in the blending operation process, and the detection accuracy is higher compared with the traditional bubble blending method.

And the specific protein analyzer that this application provided can come the control to be followed the interior liquid measure of siphoning out to mixing passageway 51 of mixing pond 30 and the liquid measure of pushing into mixing pond 30 with mixing passageway 51 through controlling mixing power device 52 to it is controllable to dilute the ratio, can not cause the influence to the testing result.

During detection, the first end 511 of the mixing passage 51 is below the liquid level of the mixed sample liquid in the mixing pool 30, so that the mixing power device 52 can suck the liquid out of the mixing pool 30 through the mixing passage 51.

In one embodiment, the blending power device 52 is configured as a syringe, a constant displacement pump, or a positive or negative pressure source for providing a negative pressure that drives the liquid in the blending passage 51 in a direction away from the blending tank 30 or a positive pressure that drives the liquid in the blending passage 51 in a direction closer to the blending tank 30.

As shown in FIG. 3, in one embodiment, the blending power plant 52 is connected to the second end of the blending passage 51 through a first control valve 70 to connect or disconnect the blending power plant to the blending passage. In this embodiment, the blending power plant 52 is configured as an injector.

In addition, the specific protein analyzer further comprises a liquid source 60, wherein the liquid source 60 is connected with the blending power device 52 and is used for providing liquid for the blending power device 52 and further providing liquid for the blending channel 51. The liquid is a diluent. The liquid source 60 may be a liquid reservoir having a diluent stored therein.

In one embodiment, as shown in FIG. 3, the source 60 is also connected to the blending power plant 52 through a first control valve 70. In this embodiment, the first control valve 70 is configured as a three-way valve, such as a three-way solenoid valve including a spool. The first control valve 70 includes a first port 71, a second port 72, and a third port 73. The first port 71 is connected with the blending power device 52, the second port 72 is connected with the liquid source 60, and the third port 73 is connected with the blending passage 51. The first control valve 70 controls the on-off of the blending power device 52 and the blending channel 51 by controlling the on-off of the first interface 71 and the third interface 73, so as to determine whether the blending power device 52 can drive the liquid in the blending channel 51 to move; the first control valve 70 controls the on-off of the liquid source 60 and the blending power device 52 by controlling the on-off of the first interface 71 and the second interface 72.

When the blending operation needs to be performed, the first control valve 70 controls the first interface 71 and the third interface 73 to be communicated, so that the blending power device 52 sucks and pushes the liquid by providing positive pressure or negative pressure, the mixed sample liquid is blended, and at the moment, the first interface 71 of the first control valve 70 is disconnected from the second interface 72. When the blending operation is not needed, the first control valve 70 controls the first interface 71 and the third interface 73 to be disconnected, the blending power device 52 cannot provide positive pressure or negative pressure for the liquid in the blending passage 51 to suck and push the liquid, and at the moment, the first interface 71 and the second interface 72 of the first control valve 70 are connected.

In addition, when the first port 71 and the second port 72 of the first control valve 70 are connected, the blending power device 52 can suck the liquid from the liquid source, so that the liquid can be supplied to the blending passage 51, and the blending passage 51 is filled with the liquid.

Of course, in another embodiment, the hydraulic pressure 60 may be connected to the blending power plant 52 through a second control valve (not shown) separate from the first control valve 70.

In one embodiment, as shown in fig. 2, the detection area 41 is disposed on the blending pool 30, that is, the detection device 40 is disposed integrally with the blending pool 30, so that the structure and the arrangement are simple and cost-saving.

Specifically, a part of the mixing cell 30 is selected as the detection region 41. Portions of the detection zone 41 are made of a light transmissive material. The mixing well 30, except for the detection region 41, may or may not be made of a light-transmitting material, and is not limited thereto. After the mixed sample solution is mixed, the light source 42 is aligned with the detection area 41 on the mixing cell 30 for detection.

The detection area 41 is arranged corresponding to the mixed sample solution in the mixing well 30.

In other embodiments, a structure dedicated to detection may be provided in the mixing tank 30 as the detection area 41. For example, a light-transmitting line structure is provided in the kneading tank 30, which communicates with the region for kneading. After the sample liquid is uniformly mixed, the uniformly mixed sample liquid directly flows into the light transmission pipeline, and the light source irradiates the mixed sample liquid flowing to the corresponding position to perform detection, which is not limited herein.

In one embodiment, referring to fig. 4, the detection area 41 of the detection device 40 is a part of the mixing channel 51, when performing the detection operation, the mixing power device 52 sucks the mixed sample solution in the mixing pool 30 into the mixing channel 51, and the light source 42 can irradiate the mixed sample solution flowing through the part of the mixing channel 51 forming the detection area 41, so as to detect the content of the specific protein in the mixed sample solution.

The portion of the mixing channel 51 other than the detection area 41 may be made of a light-transmitting material, or may not be made of a light-transmitting material, and is not limited herein.

In this embodiment, set up detection zone 41 on mixing passageway 51, mixing passageway 51 sets up with detection zone 41 integral type structure promptly, and simple structure practices thrift the cost, and sets up detection zone 41 on mixing passageway 51 and make the mixed sample liquid flow in detection zone 41 when, the journey of flowing is long, and the testing result accuracy is high. And the occupied space can be reduced by bending the uniform mixing channel 51, so that the whole volume of the specific protein analyzer is small.

In one embodiment, referring to FIG. 5, the detection zone 41 is configured as a detection channel 80 separate from the mixing channel 51. The detection channel 80 is communicated with the inner space of the blending pool 30 and is filled with liquid, and the detection channel 80 is used for allowing the blended sample liquid to flow in, so that the light source 42 can irradiate the blended sample liquid flowing into the detection channel 80 to detect the content of the specific protein. The structure of the detection channel 80 can be referred to the above description of the mixing channel 51, and is not described herein again.

The specific protein analyzer further comprises a detection driving device 81, the detection driving device 81 is connected with the detection channel 80, and the detection driving device 81 is used for driving liquid in the detection channel 80 to flow in a direction away from the blending pool 30, so that the blended sample liquid in the blending pool 30 is sucked out of the detection channel 80 for specific protein detection.

The detection driving device 81 is further configured to drive the liquid in the detection channel 80 to flow in a direction close to the blending pool 30, and push the mixed sample liquid detected in the detection channel 80 into the blending pool 30 to wait for processing.

The detection operation process of the specific protein analyzer provided by the embodiment is as follows:

after the mixed sample liquid in the blending cell 30 is blended, the detection driving device 81 firstly provides negative pressure to drive the liquid in the detection channel 80 to flow in the direction far away from the blending cell 30, the mixed sample liquid blended in the blending cell 30 is sucked out of the detection channel 80, the light source 42 irradiates the mixed sample liquid flowing to the corresponding position, and the content of the specific protein is detected by monitoring the change of the transmission and/or scattered light signals.

After the detection, the detection driving device 81 provides positive pressure to drive the liquid in the detection channel 80 to flow in the direction close to the blending pool 30, and pushes the mixed sample liquid in the detection channel 80 into the blending pool 30 to be processed.

In the present embodiment, the detection drive can also be configured as a syringe, a metering pump or a positive or negative pressure source.

In one embodiment, as shown in FIG. 5, the blending power unit 52 and the detection drive unit are the same drive unit, and the blending power unit 52 is also connected to the detection path 80, such as through the third control valve 74 to the detection path 80. In the embodiment shown in FIG. 5, the blending power plant 52 is connected to the blending passage 51, the detection passage 80, or the fluid source 60 by controlling the first control valve 70 and the third control valve 74 (both three-way valves, for example). Of course, in other embodiments, the blending power device 52 and the detection driving device 81 may be different driving devices, as shown in fig. 6 and 7. In the embodiment shown in fig. 6, the detection drive 81 is connected to the further fluid source 61 via the third control valve 74; in the embodiment shown in fig. 7, the detection drive 81 is connected to the liquid source 60 via the third control valve 74, i.e., the detection drive 81 and the mixing power unit 52 are connected to the same liquid source 60 via the respective control valves.

In one embodiment, the fluid after testing needs to be drained from the mixing well 30 to empty the mixing well 30, so that the mixing well 30 can be used to make room for testing the next blood sample to be tested. Further, the specific protein analyzer further includes a waste liquid channel 90 and a waste liquid driving device 100. Both ends of the waste liquid channel 90 are respectively connected with the inner space of the mixing tank 30 and the waste liquid driving device 100. Similarly, the waste liquid channel 90 is filled with liquid to empty the gas in the waste liquid channel 90, so as to avoid the gas from entering the mixing pool 30 to form bubbles and influence the accuracy of the detection result. The waste liquid driving device 100 is used for discharging the liquid in the mixing tank 30 through the waste liquid channel 90. Specifically, the waste liquid driving device 100 drives the liquid in the waste liquid channel 90 to flow in the direction away from the mixing tank 30, so as to suck the liquid in the mixing tank 30 out of the waste liquid channel 90, thereby performing waste liquid discharge operation.

In one embodiment, the blending power device 52 and the waste fluid driving device 100 may be the same driving device, and the blending power device 52 is also connected to the waste fluid channel 90, for example, through the fourth control valve 75 and the waste fluid channel 90. Of course, in other embodiments, the blending power device 52 and the waste fluid drive device 100 may be different drive devices.

In one embodiment, when the detection region 41 is configured as the detection channel 80 separate from the mixing channel 51, the mixing channel 51 and the waste channel 90 may be configured as a single unit, and the mixing power device 52 and the waste driving device 100 may be the same driving device. That is, the waste liquid channel 90 and the waste liquid driving device 100 are used for both discharging the waste liquid and performing suction-push mixing.

In one embodiment, the sample supply apparatus 10 and the reagent supply apparatus 20 are configured as the same pipette needle, or as a sample needle and a reagent needle that are independent of each other.

In one embodiment, the specific protein analyzer further includes a hemolytic agent supply device 110, the hemolytic agent supply device 110 includes a hemolytic agent supply channel 111 and a hemolytic agent driving device 112, the hemolytic agent supply channel 111 is communicated with the mixing pool 30, and the hemolytic agent supply channel 111 is used for supplying hemolytic agent into the mixing pool 30 to react with the blood sample to be tested.

The hemolytic agent driving device 112 is used for driving hemolytic agent to enter the blending tank 30 through the hemolytic agent supply channel 111.

In one embodiment, the hemolytic agent driving device 112 may be the same driving device as the mixing power device 52 or may be a different driving device. Similarly, the hemolytic agent driving device 112 is connected to the hemolytic agent supply channel 111 through a control valve to connect or disconnect the hemolytic agent supply channel 111.

In one embodiment, referring to fig. 8, the specific protein analyzer further comprises a blood routine detection module 120 for classifying and/or counting cells in the blood sample to be tested. Specifically, the blood routine detection module 120 may include at least one of a WBC (white blood cell) classification measurement module, a WBC/HGB measurement module, and an RBC/PLT measurement module. The WBC classification measurement module is used for obtaining a five-classification result of WBCs of a blood sample to be measured, the WBC/HGB measurement module is used for completing WBC counting and morphological parameter measurement and has a function of measuring HGB (hemoglobin), and the RBC/PLT measurement module is used for completing RBC (red blood cell), PLT (blood platelet) counting and morphological parameter measurement.

As shown in fig. 8, the specific protein analyzer further includes a controller 130 electrically connected to the sample supply device 10, the reagent supply device 20, and the mixing power device 52, and configured to control the sample supply device 10, the reagent supply device 20, and the mixing power device 52, respectively. Further, the controller 130 is also electrically connected to each control valve.

The working process of the specific protein analyzer provided by one embodiment of the application is as follows:

the controller 130 controls the sample supply device 10 to add the blood sample to be tested into the mixing pool 30.

When the blood sample to be tested is a whole blood sample, the controller 130 controls the hemolytic agent driving device 112 to drive the hemolytic agent in the hemolytic agent supply channel 111 to enter the mixing pool 30, react with the blood sample to be tested in the mixing pool 30, and dissolve blood cells of the blood sample to be tested in the mixing pool 30. After the blood cells are dissolved, the controller 130 controls the reagent supplying device 20 to add the latex reagent into the mixing pool 30, so as to form a mixed sample solution in which the latex reagent and the blood sample to be detected are not fully mixed.

After the blending operation is started, the controller 130 controls the first control valve 70 to communicate the blending power device 52 with the blending passage 51, and the controller 130 controls the blending power device 52 to perform the following multiple suction-pushing blending operations:

the controller 130 firstly controls the blending power device 52 to provide negative pressure, so as to drive the liquid in the blending channel 51 to flow in a direction away from the blending pool 30, and further suck a part of the mixed sample liquid in the blending pool 30 out of the blending channel 51.

Then, the controller 130 controls the blending power device 52 to provide positive pressure, so as to drive the liquid in the blending passage 51 to flow in a direction close to the blending tank 30, and further push a part of the liquid in the blending passage 51 into the blending tank 30.

And repeating the circulation until the blood sample to be detected and the latex reagent in the mixing pool 30 are mixed uniformly to be detected.

If the detection area 41 is a part of the blending channel 51, after the blending operation is finished, the controller 130 controls the blending power device 52 to drive the liquid in the blending channel 51 to flow in a direction away from the blending pool 30, so that the mixed sample liquid blended in the blending pool 30 is sucked out of the detection area 41 of the blending channel 51, and the light source 42 irradiates the mixed sample liquid flowing through the detection area 41. The content of the specific protein in the blood sample to be detected is detected by monitoring the change rate of the transmission and/or scattered light signals and certain calculation.

After the detection operation is finished, the controller 130 controls the blending power device 52 to drive the liquid in the blending channel 51 to flow in the direction close to the blending pool 30, and pushes the liquid in the blending channel 51 into the blending pool 30 to wait for processing.

Finally, the controller 130 controls the waste liquid driving device 100 to drive the liquid in the waste liquid channel 90 to flow in a direction away from the mixing pool 30, so that the detected mixed sample liquid in the mixing pool 30 is discharged through the waste liquid channel 90, and the mixing pool 30 is emptied to detect a next blood sample to be detected.

Of course, in one embodiment, a particular protein analyzer may also include a washing assembly (not shown) for washing the homogenization channel and/or the detection channel and/or the waste channel after the detection operation is completed to prevent cross-contamination.

In one embodiment, the present application further provides a blending method applied to the blending device according to any one of the above embodiments. Specifically, referring to fig. 10, the blending method includes the following steps:

step S200, the sample supply device 10 and the reagent supply device 20 respectively add the blood sample to be tested and the latex reagent into the mixing pool 30 to form a mixed sample solution.

In step S210, the kneading power device 52 performs at least one suction/push kneading operation including step S211 and step S212.

Step S211, the liquid in the blending passage 51 is driven to flow in a direction away from the blending pool 30, so as to suck the liquid in the blending pool 30 into the blending passage 51.

Step S212, the liquid in the blending passage 51 is driven to flow in a direction close to the blending pool 30, so as to push the liquid in the blending passage 51 into the blending pool 30, thereby forming a rotational flow in the blending pool 30 to blend the blood sample to be tested and the latex reagent.

And performing suction-push mixing operation for multiple times until the blood sample to be detected and the latex reagent are mixed uniformly.

In each of the sucking-pushing kneading operations, the liquid pushed into the kneading cell 30 by the kneading power unit 52 may include the sample liquid to be measured sucked into the kneading passage 51 by the kneading power unit 52 and the liquid (e.g., diluent) originally filled in the kneading passage 51.

Preferably, in each sucking and pushing mixing operation, the volume of the liquid sucked into the mixing channel 51 by the mixing power device 52 is smaller than the volume of the liquid pushed into the mixing pool 30 by the mixing power device 52, so that the mixed sample liquid does not remain in the mixing channel 51, and the loss of the mixed sample liquid is prevented from influencing the accuracy of the detection result.

Preferably, in each sucking and pushing mixing operation, the volume of the liquid sucked into the mixing channel 51 by the mixing power device 52 is greater than or equal to one fourth of the volume of the liquid in the mixing pool 30, so that when the liquid sucked into the mixing channel 51 is pushed into the mixing pool 30 again, a rotational flow can be formed in the mixing pool 30, and the phenomenon that the liquid sucked and pushed is too small to form the rotational flow and cannot perform the mixing function is avoided.

In one embodiment, the kneading power unit 52 performs a plurality of suction-push kneading operations, and in each suction-push kneading operation, the volume of the liquid sucked into the kneading passage 51 by the kneading power unit 52 is the same as or different from the volume of the liquid pushed into the kneading tank 30 by the kneading power unit 52. That is, the volume of the liquid sucked into the kneading passage 51 by the kneading power unit 52 and the volume of the liquid pushed into the kneading tank 30 by the kneading power unit 52 can be adjusted according to actual conditions in each sucking-pushing operation, and is not limited to the same.

In one embodiment, the blood sample to be tested is a whole blood sample, and before the reagent supplying device 20 adds the latex reagent into the mixing pool 30, the mixing method further includes: the hemolytic agent is added to the mixing tank 30.

In an embodiment, referring to fig. 10, a blending method provided in the embodiment of the present application includes the following steps:

and step S300, adding the whole blood sample to be detected into the mixing pool 30.

In step S310, a hemolytic agent is added to the mixing pool 30 to dissolve blood cells in the sample.

Step S320, adding an emulsion reagent into the mixing cell 30 to form a mixed sample solution.

In step S330, the kneading power device 52 performs at least one suction/push kneading operation including step S331 and step S332.

Step S331, the liquid in the blending passage 51 is driven to flow in a direction away from the blending pool 30, so as to suck the liquid in the blending pool 30 into the blending passage 51.

Step S332, driving the liquid in the blending passage 51 to flow in a direction close to the blending pool 30, so as to push the liquid in the blending passage 51 into the blending pool 30, thereby forming a rotational flow in the blending pool 30 to blend the hemolyzed whole blood sample to be detected and the latex reagent.

In the description of the present application, it should be noted that the terms "upper", "lower", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, which are only for convenience in describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and operate, and thus, should not be construed as limiting the present application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; the communication may be direct, indirect via an intermediate medium, or internal to both elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art. In addition, in the description of the present application, "a plurality" means two or more unless otherwise specified.

The present invention is not intended to be limited to the particular embodiments shown and described, but is to be accorded the widest scope consistent with the principles and novel features herein disclosed.

Claims

1. A specific protein analyzer comprising:

the sample supply device is used for providing a blood sample to be tested;

2. The specific protein analyzer of claim 1, wherein the mixing power device is configured as an injector, a quantitative pump or a positive/negative pressure source, and is configured to provide a negative pressure for driving the liquid in the mixing channel to flow in a direction away from the mixing pool or a positive pressure for driving the liquid in the mixing channel to flow in a direction close to the mixing pool.

3. The specific protein analyzer of claim 1 or 2, wherein the mixing power device is connected to the second end of the mixing channel through a first control valve, so as to connect or disconnect the mixing power device to the mixing channel.

4. The specific protein analyzer of claim 3, further comprising a liquid source connected to the mixing power device, the liquid source being configured to provide liquid to the mixing power device, and further being configured to provide liquid to the mixing channel.

5. The specific protein analyzer of claim 4, wherein the liquid source is connected to the mixing power device via the first control valve, and the control valve comprises a first port, a second port, and a third port;

6. The specific protein analyzer of claim 4, wherein the fluid source is connected to the mixing power device via a second control valve to connect or disconnect the mixing power device to the fluid source.

7. The specific protein analyzer of any one of claims 1 to 6, wherein the detection zone is disposed on the mixing well.

8. The specific protein analyzer according to any one of claims 1 to 6, wherein the detection area of the detection device is a part of the mixing channel, the mixing power device sucks the mixed sample solution in the mixing cell out of the mixing channel during detection, and the light source can irradiate the mixed sample solution flowing through the part of the mixing channel constituting the detection area, so as to detect the specific protein content in the mixed sample solution.

9. The specific protein analyzer according to any one of claims 1 to 6, wherein the detection area is constituted as a detection channel independent from the mixing channel, the detection channel being in communication with the inner space of the mixing well and being filled with a liquid;

the specific protein analyzer also comprises a detection driving device, and the detection driving device is connected with the detection channel; the detection driving device is used for driving the liquid in the detection channel to flow in the direction far away from the mixing pool, and the mixed sample liquid mixed in the mixing pool is sucked out of the detection channel to be subjected to specific protein detection.

10. The specific protein analyzer of claim 9, wherein the mixing power device and the detection driving device are the same driving device, and the mixing power device is connected to the detection channel through a third control valve, so as to connect or disconnect the mixing power device and the detection channel.

11. The specific protein analyzer according to any one of claims 1 to 10, further comprising a waste liquid channel and a waste liquid driving device, wherein both ends of the waste liquid channel are respectively connected to the inner space of the mixing tank and the waste liquid driving device; and the waste liquid driving device is used for discharging the liquid in the mixing pool through the waste liquid channel.

12. The specific protein analyzer according to any one of claims 1 to 11, wherein the sample supply means and the reagent supply means are constituted as the same pipette needle, or are constituted as a sample needle and a reagent needle independent of each other.

13. The specific protein analyzer according to any one of claims 1 to 12, further comprising a hemolytic agent supply device comprising a hemolytic agent supply channel; the hemolytic agent supply channel is communicated with the blending pool and is used for supplying hemolytic agent to the blending pool to react with the blood sample to be tested.

14. The specific protein analyzer according to any one of claims 1 to 13, further comprising a blood routine detection module for classifying and/or counting cells in the blood sample to be tested.

15. The specific protein analyzer according to any one of claims 1 to 14, further comprising a controller electrically connected to the sample supply device, the reagent supply device, and the kneading power device, respectively, and configured to control the sample supply device, the reagent supply device, and the kneading power device, respectively.

16. A method of uniform mixing, which is applied to the specific protein analyzer according to any one of claims 1 to 15,

the uniformly mixing method comprises the following steps:

17. The kneading method according to claim 16, wherein the volume of the liquid sucked into the kneading passage by the kneading power unit is smaller than the volume of the liquid pushed into the kneading tank by the kneading power unit in each of the sucking-pushing kneading operations.

18. The kneading method according to claim 16 or 17, wherein the volume of the liquid sucked into the kneading passage by the kneading power unit is equal to or larger than one-fourth of the volume of the liquid in the kneading tank in each of the suction-push kneading operations.

19. The kneading method according to any one of claims 16 to 18, wherein the kneading power unit performs the suction-push kneading operation a plurality of times, and in each of the suction-push kneading operations, the volume of the liquid sucked into the kneading passage by the kneading power unit is the same as or different from the volume of the liquid pushed into the kneading tank by the kneading power unit.

20. The blending method according to any one of claims 16 to 19, wherein the blood sample to be tested is a whole blood sample, and before the reagent supplying device adds the latex reagent into the blending pool, the method further comprises:

and adding a hemolytic agent into the mixing pool.