CN114018787B - Particle detection unit, mixing system and mixing method - Google Patents
Particle detection unit, mixing system and mixing method Download PDFInfo
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- CN114018787B CN114018787B CN202111236637.7A CN202111236637A CN114018787B CN 114018787 B CN114018787 B CN 114018787B CN 202111236637 A CN202111236637 A CN 202111236637A CN 114018787 B CN114018787 B CN 114018787B
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- 238000001514 detection method Methods 0.000 title claims abstract description 78
- 239000002245 particle Substances 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000002347 injection Methods 0.000 claims abstract description 118
- 239000007924 injection Substances 0.000 claims abstract description 118
- 239000000758 substrate Substances 0.000 claims abstract description 59
- 239000000126 substance Substances 0.000 claims abstract description 25
- 238000007664 blowing Methods 0.000 claims description 136
- 239000007788 liquid Substances 0.000 claims description 48
- 239000012491 analyte Substances 0.000 claims description 28
- 239000000463 material Substances 0.000 claims description 17
- 238000007789 sealing Methods 0.000 claims description 13
- 238000004891 communication Methods 0.000 claims description 9
- 238000004378 air conditioning Methods 0.000 claims description 3
- 230000002209 hydrophobic effect Effects 0.000 claims description 3
- 238000011896 sensitive detection Methods 0.000 claims description 3
- 230000004907 flux Effects 0.000 claims 1
- 238000005086 pumping Methods 0.000 abstract description 18
- 230000008569 process Effects 0.000 abstract description 7
- 239000000203 mixture Substances 0.000 abstract description 4
- 238000004043 dyeing Methods 0.000 description 8
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 3
- 239000004926 polymethyl methacrylate Substances 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000565 sealant Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
- G01N2001/387—Other diluting or mixing processes mixing by blowing a gas, bubbling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Abstract
The present application relates to a particle detection unit, a mixing system and a mixing method, the particle detection unit comprising: the device comprises a substrate, a sample injection device, a flow channel and a suction device, wherein the sample injection device and the suction device are arranged on the substrate, the flow channel is embedded in the substrate, the sample injection device is provided with a sample injection port, and the sample injection device is provided with an outlet; one end of the flow channel is communicated with the sample injection device through the outlet; the suction device is connected with the sample injection device through a flow channel. The user does not need to manually mix the samples; the color substance can be placed in the particle detection unit and positioned in the path in front of the detection window, and the sample can be directly mixed with the color substance in the process of pumping and flowing through the pumping device by the pumping assembly, so that the step of manually mixing the color substance is avoided, and the detection efficiency is greatly improved.
Description
Technical Field
The application relates to the technical field of cell detection, in particular to a particle detection unit, a mixing system and a mixing method.
Background
In the biotechnology field, cell detection is usually performed by using card-type consumables, manual sample loading is required to be performed on the consumables, and samples are required to be fully mixed manually before sample loading; the multi-channel consumable material needs to prepare color substances by itself, and is manually injected into a detection window after manually mixing sample liquid and liquid color substances, and then is placed under a fluorescence microscope for observation and counting; thus, the cell detection efficiency is low.
Disclosure of Invention
In view of the above, it is desirable to provide a particle detection unit, a mixing system, and a mixing method that can improve detection efficiency.
A particle detection unit, the particle detection unit comprising:
a substrate;
the sample injection device is arranged on the substrate and is provided with a sample injection port and an outlet;
the flow channel is embedded in the substrate, and one end of the flow channel is communicated with the sample injection device through the outlet; a kind of electronic device with high-pressure air-conditioning system
The suction device is arranged on the substrate and is connected with the sample injection device through a flow channel.
In one embodiment, the flow channel may allow at least a portion of the liquid analyte to flow within the channel, the flow channel having a bend or obstruction at least a portion thereof such that the liquid analyte is at the bend or obstruction for adjusting the liquid analyte flow rate to extend the flow path.
In one embodiment, the particle detection unit further comprises a blowing and sucking device, the blowing and sucking device is arranged on the substrate, the blowing and sucking device is provided with a blowing and sucking mixing port, and the sample injection device is provided with sample injection mixing ports which are mutually spaced from the outlet; one end of one flow channel is communicated with the blowing and sucking device through the blowing and sucking mixing port, and the other end is communicated with the sample injection device through the sample injection mixing port.
In one embodiment, the flow channel has at least two branches, which are connected to and disconnected from the suction device by valve control.
In one embodiment, the substrate is a sheet material that can encapsulate at least a portion of the flow channel.
In one embodiment, the flow channel has a flow passage equivalent diameter in the range of 10-1000 μm.
In one embodiment, the suction device comprises a suction cavity arranged on the substrate and a sealing plug arranged on the suction cavity, and the suction cavity is communicated with the flow channel; the sealing plug is used for being connected with a suction assembly for providing suction power.
In one embodiment, the particle detecting unit further includes a detecting window, the detecting window is disposed on the substrate, one end of the detecting window is communicated with the flow channel, and the other end of the detecting window is communicated with the suction device.
In one embodiment, the other end of the detection window is communicated with the suction device through a connecting channel.
In one embodiment, a moisture sensitive detection member is disposed within the connection channel.
In one embodiment, a filter is disposed within the suction device.
In one embodiment, the particle detecting unit further includes a color substance disposed in the flow channel, or disposed in the sample injection device, or disposed in the suction device, or disposed in the blowing-sucking device, and the color substance is used for mixing with the liquid analyte injected into the sample injection device.
A mixing system, comprising:
the particle detection unit; a kind of electronic device with high-pressure air-conditioning system
The suction assembly comprises a suction power source, a suction device and a suction needle-shaped device, wherein the suction needle-shaped device is used for being inserted into the suction device and communicated with the suction device, and the suction power source is used for driving the suction device to act so as to suck air into the suction device through the suction needle-shaped device.
In one embodiment, the suction power source is a first linear stepper motor, the suction device comprises a first cylinder body and a first piston rod, one end of the first piston rod is arranged in the first cylinder body, the other end of the first piston rod is arranged at the output end of the first linear stepper motor, and the suction needle-shaped device is communicated with the first cylinder body.
In one embodiment, the mixing system further comprises a blowing and sucking assembly, the blowing and sucking assembly comprises a blowing and sucking power source, a blowing and sucking device and a blowing and sucking needle-shaped device, the blowing and sucking needle-shaped device is used for being inserted into the blowing and sucking device and communicated with the blowing and sucking device, and the blowing and sucking power source is used for driving the blowing and sucking device to act so as to blow or suck air through the blowing and sucking needle-shaped device.
In one embodiment, the blowing and sucking power source is a second linear stepper motor, the blowing and sucking device comprises a second cylinder body and a second piston rod, one end of the second piston rod is arranged in the second cylinder body, and the other end of the second piston rod is arranged at the output end of the second linear stepper motor; the blowing and sucking needle-shaped device is communicated with the second cylinder body.
In one embodiment, the mixing system further comprises a third power source and a driving plate, wherein the suction needle-shaped device and the blowing and sucking needle-shaped device are arranged on the driving plate, and the third power source is used for driving the driving plate to move along the direction approaching to or separating from the base plate.
A mixing method comprising the steps of:
injecting liquid analyte into the sample injection device through a sample injection port of the sample injection device, wherein the sample injection device is arranged on a substrate, a flow channel is arranged in the substrate, and a suction device communicated with the sample injection device through the flow channel is also arranged on the substrate;
inserting a suction needle device into the suction device;
the suction device is sucked through the suction needle-shaped device by driving the suction device to act through the suction power source, so that liquid analytes in the sample injection device enter the flow channel.
A mixing method comprising the steps of:
injecting liquid analyte into the sample injection device through a sample injection port of the sample injection device, wherein the sample injection device is arranged on a substrate, a flow channel is arranged in the substrate, and a suction device and a blowing and sucking device which are respectively communicated with the sample injection device through the flow channel are also arranged on the substrate;
inserting the blowing and sucking needle-shaped device into the blowing and sucking device;
the blowing and sucking device is driven by the blowing and sucking power source to act so as to blow or suck air to the blowing and sucking device through the blowing and sucking needle-shaped device, so that liquid analytes in the sample injection device flow into the blowing and sucking device through the flow channel or flow into the sample injection device through the flow channel, and the liquid analytes are uniformly mixed;
inserting a suction needle device into the suction device;
the aspirator is driven by the aspiration power source to act so as to aspirate the aspiration device through the aspiration needle-shaped device, so that the evenly mixed liquid analyte in the sample injection device enters the flow channel.
In one embodiment, the mixing method further includes the following steps:
and a color substance is arranged in the flow channel, the sample injection device, the suction device or the blowing and sucking device, and the color substance is mixed with the liquid analyte.
The particle detection unit, the mixing system and the mixing method have at least the following advantages:
adding a sample into a sample injection device through a sample injection port, inserting a suction needle-shaped device of a suction assembly into the suction device, sucking air from the suction device through the suction needle-shaped device by a suction power source of the suction assembly, sucking the sample in the sample injection device into a flow channel of a substrate through an outlet, blowing air from the suction power source of the suction assembly into the suction device through the suction needle-shaped device, reversely blowing the sample in the flow channel into the sample injection device, and repeatedly sucking air and blowing air for a plurality of times by the sample, so that full mixing can be realized; in addition, the suction power source of the suction assembly pumps the suction device through the suction needle-shaped device, and the sample in the sample feeding device is pumped into the flow channel of the substrate through the outlet and finally flows to the detection window for detection. Therefore, the sample is directly injected into the sample injection device, and a user does not need to manually mix the sample; the color substances can be placed in the particle detection unit and positioned in the path in front of the detection window, and the sample can be directly mixed with the color substances in the process of pumping and flowing through the pumping device by the pumping assembly, so that the step of manually mixing the color substances is omitted, and the detection efficiency is greatly improved.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application.
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a mixing system in one embodiment;
FIG. 2 is a top view of a sample plate in one embodiment;
FIG. 3 is a top view of the particle detection unit of the sample plate of FIG. 2;
FIG. 4 is a side view of a particle detection unit of the sample plate of FIG. 2;
FIG. 5 is a flow diagram of a hybrid method in one embodiment;
fig. 6 is a flow diagram of a hybrid method in another embodiment.
Reference numerals illustrate:
10. a mixing system; 100. a sample plate; 200. a suction assembly; 300. a blowing and sucking component; 400. a particle detection unit; 410. a substrate; 420. a sample introduction device; 430. a blowing and sucking device; 440. a second microchannel; 450. a first microchannel; 460. detecting a window; 470. a suction device; 421. a sample inlet; 422. an outlet; 431. blowing and sucking a mixing port; 423. a sample injection mixing port; 480. a connection channel; 490. a filter; 481. a moisture sensitive detection member; 210. a suction power source; 220. an aspirator; 230. sucking the needle-shaped device; 471. a first sealing plug; 310. a blowing and sucking power source; 320. a blowing and sucking device; 330. a blowing and sucking needle-shaped device; 432. a second sealing plug; 500. a third power source; 600. and a driving plate.
Detailed Description
In order that the above objects, features and advantages of the application will be readily understood, a more particular description of the application will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. The present application may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the application, whereby the application is not limited to the specific embodiments disclosed below.
Referring to fig. 1 and 2, a mixing system 10 in one embodiment includes a particle detection unit 400 and a pumping assembly 200. The aspiration assembly 200 mixes or stains the sample injected into the particle detection unit 400 by aspirating the sample plate 100.
Specifically, the sample plate 100 includes a plurality of particle detection units 400. For example, referring to fig. 2 together, the sample plate 100 includes 24 particle detection units 400, where the 24 particle detection units 400 are distributed in 2 rows of 12 particles each, so as to form a sample plate 100 with 24 channels, and the 24 particle detection units 400 can be sequentially detected, so that the working efficiency is improved. Of course, in other embodiments, the sample plate 100 may further include other numbers of particle detection units 400, for example, 2, 3, 10, 20, etc., and the number and arrangement positions of the particle detection units 400 may be set according to actual needs.
Referring to fig. 3 and 4, the particle detecting unit 400 includes a substrate 410, a sample injection device 420, a flow channel, and a suction device 470. Further, the particle detecting unit 400 further includes a detecting window 460 and a blowing and sucking device 430. The substrate 410 may be made of Polystyrene (PS), polycarbonate (PC) or polymethyl methacrylate (polymethyl methacrylate, PMMA). The base plate 410 of all the particle detection units 400 on the sample plate 100 is integrally formed.
In an embodiment, the sample injection device 420 is disposed on the substrate 410, and a sample injection port 421 is disposed on top of the sample injection device 420. Specifically, the top of the sample injection device 420 is opened to form a sample injection port 421, so that the convenience of sample injection is improved. Of course, in other embodiments, the top of the sample injection device 420 may be provided with a sample inlet 421, and the size of the sample inlet 421 is smaller than the inner diameter of the top of the sample injection device 420. The sample injection device 420 is provided with an outlet 422. Specifically, the outlet 422 is formed on the bottom surface of the sample injection device 420. Of course, in other embodiments, the outlet 422 may also be formed on the side of the bottom of the sample injector 420. Alternatively, in another embodiment, the outlet 422 may be further formed on a side of the middle of the sample injection device 420, so long as the sample in the sample injection device 420 can smoothly enter the flow channel. In the present embodiment, the number of outlets 422 is one. Of course, in other embodiments, the number of outlets 422 may be what.
Optionally, the flow channel includes a first fluidic channel 450 and a second fluidic channel 440. The first fluidic channel 450 is not in direct communication with the second fluidic channel 440. For example, in fig. 4, the first micro flow channel 450 is located below the second micro flow channel 440, and both the first micro flow channel 450 and the second micro flow channel 440 form a double flow channel structure.
Specifically, the first micro flow channel 450 is embedded in the substrate 410, and one end of the first micro flow channel 450 is communicated with the sample injection device 420 through the outlet 422. The first micro flow channel 450 has a color substance embedded therein. Specifically, the diameter of the first micro flow channel 450 may range from 10 micrometers to 1000 micrometers. The first micro flow channel 450 may be formed on the substrate 410 by a micro flow channel technology, so as to be embedded in the substrate 410, thereby preventing the first micro flow channel 450 from being damaged due to exposure. The color material in the first micro flow channel 450 is sprayed in the first micro flow channel 450 by spraying, and the color material is used for dyeing the sample.
Further, the first micro flow channel 450 is bent to extend the dyeing path, thereby improving the dyeing effect. For example, in fig. 3, the first micro flow channel 450 includes a plurality of straight line segments, and the straight line segments are bent and connected to form the first micro flow channel 450. Of course, in other embodiments, the first micro flow channel 450 may also be an arc segment or the like.
Further, a detection window 460 is disposed on the substrate 410, and one end of the detection window 460 is in communication with the first micro flow channel 450. The inspection window 460 is primarily for the camera to take a picture, and therefore the inspection window 460 is transparent.
Further, a suction device 470 is disposed on the substrate 410, and the other end of the detection window 460 is in communication with the suction device 470. For example, the other end of the inspection window 460 is in communication with the suction device 470 through a connection channel 480. The connecting channel 480 may also have a diameter ranging between 10 microns and 1000 microns.
Optionally, a filter 490 is disposed within the suction device 470. The filter 490 is provided with a hydrophobic and air-permeable material, and when the liquid in the first micro flow channel 450 reaches the filter 490, the liquid in the whole first micro flow channel 450 is kept in a non-flowing state or a slow flowing state through the filter 490, so that the liquid in the detection window 460 is ensured to be non-flowing or slow flowing, and the observation is facilitated.
Further, a humidity-sensitive detecting member 481 is provided in the connection channel 480, and when the liquid in the first micro flow channel 450 passes through the humidity-sensitive detecting member 481, the humidity-sensitive detecting member 481 changes color, proving that the particle detecting unit 400 has been used. For example, the moisture-sensitive detecting member 481 may be a moisture-sensitive detecting paper.
Optionally, the mixing system 10 further includes a blowing and sucking assembly 300, and the blowing and sucking assembly 300 mixes the sample in the particle detecting unit 400 by sucking air or blowing air. The blowing and sucking device 430 is disposed on the substrate 410, and a blowing and sucking mixing port 431 is disposed at the bottom of the blowing and sucking device 430. Specifically, the blowing and sucking mixing port 431 is provided at a side of the bottom of the blowing and sucking device 430. Of course, in other embodiments, the blowing and sucking mixing port 431 may be provided on the bottom surface of the blowing and sucking device 430. The bottom of the sample injection device 420 is further provided with sample injection mixing ports 423 spaced from the outlet 422. Similarly, the sample mixing port 423 is disposed on a side surface of the bottom of the sample device 420, and the blowing and sucking mixing port 431 is disposed opposite to the sample mixing port 423. One end of the second micro flow channel 440 is communicated with the blowing and sucking device 430 through the blowing and sucking mixing port 431, and the other end is communicated with the sample injection device 420 through the sample injection mixing port 423. The blowing and sucking device 430 and the sample injection device 420 may be disposed next to each other or at intervals, so long as the blowing and sucking device 430 is ensured to be communicated with the sample injection device 420 through the second micro flow channel 440. The blowing and sucking device 430 is located between the sample injection device 420 and the sucking device 470, so that arrangement rationality is improved, and space is saved.
Further, the diameter of the second fluidic channel 440 may range from 10 micrometers to 1000 micrometers. The second micro flow channel 440 may be formed on the substrate 410 by a micro flow channel technique. For example, the second micro flow channel 440 may be embedded in the substrate 410 to prevent the micro flow channel from being damaged due to exposure.
Referring again to fig. 1, the suction assembly 200 includes a suction power source 210, a suction unit 220, and a suction needle 230, the suction needle 230 being inserted into the suction device 470. For example, the suction device 470 includes a suction cavity provided on the base plate 410 and a first sealing plug 471 provided on the suction cavity. The top of the suction device 470 is provided with a first sealing plug 471 for the insertion of the suction needle-shaped means 230. The suction needle device 230 is a steel needle. The first sealing plug 471 may be a silicone plunger or a soft sealant that facilitates insertion of the suction needle device 230 to aspirate the suction device 470. The suction needle device 230 is connected with the aspirator 220, and the suction power source 210 is used for driving the aspirator 220 to act so as to suck air from the suction device 470 through the suction needle device 230, so that the suction needle device 230 is not contacted with the liquid in the suction device 470, the suction needle device 230 is not polluted, cleaning is not needed, and the cost is reduced.
The sample is added into the sample introduction device 420 through the sample introduction port 421, the sucking needle-shaped device 230 of the sucking assembly 200 is inserted into the sucking device 470, the sucking power source 210 is used for driving the aspirator 220 to act so as to suck air into the sucking device 470 through the sucking needle-shaped device 230, the sample in the sample introduction device 420 is sucked into the first micro-channel 450 through the outlet 422, and the sample is dyed in the first micro-channel 450 and flows into the detection window 460 under the sucking action of the aspirator 220 due to the fact that the color substances are embedded in the first micro-channel 450, finally the sample reaches the filter 490, after the liquid in the first micro-channel 450 reaches the filter 490, the liquid in the whole first micro-channel 450 is kept in a non-flowing state or a slow flowing state through the filter 490, so that the liquid in the detection window 460 is ensured to be no longer flowing or slowly flowing, and the detection window 460 is convenient to observe and count through a microscope.
Specifically, the suction power source 210 may be a first linear stepper motor, the aspirator 220 includes a first cylinder and a first piston rod, one end of the first piston rod is disposed in the first cylinder, the other end of the first piston rod is disposed at an output end of the first linear stepper motor, and the suction needle device 230 is communicated with the first cylinder. For example, the first linear stepper motor rotates forward, thereby driving the first piston rod to extend out of the first cylinder, and the aspirator 220 aspirates the interior of the aspiration device 470. The first piston rod is driven to retract into the first cylinder by reversing the first linear stepper motor, and the aspirator 220 does not aspirate the aspiration device 470.
Specifically, the blowing and sucking assembly 300 includes a blowing and sucking power source 310, a blowing and sucking device 320, and a blowing and sucking needle device 330, wherein the blowing and sucking needle device 330 is inserted into the blowing and sucking device 430. For example, the blowing and sucking device 430 includes a blowing and sucking cavity disposed on the substrate 410 and a second sealing plug 432 disposed on the blowing and sucking cavity. The top of the suction device 430 is provided with a second sealing plug 432 for the insertion of the suction needle 330. The second sealing plug 432 may be a silicone plunger or a soft sealing gel, which may facilitate insertion of the blowing and sucking needle device 330 to blow or suck air in the blowing and sucking device 430. The blowing and sucking needle device 330 is communicated with the blowing and sucking device 320, and the blowing and sucking power source 310 is used for driving the blowing and sucking device 320 to act so as to blow or suck air to the blowing and sucking device 430 through the blowing and sucking needle device 330, so that the blowing and sucking needle device 330 cannot contact with a sample in the blowing and sucking device 430, pollution cannot be generated, cleaning is not required, and cost is reduced.
Specifically, the blowing and sucking power source 310 is a second linear stepper motor, the blowing and sucking device 320 includes a second cylinder and a second piston rod, one end of the second piston rod is disposed in the second cylinder, and the other end of the second piston rod is disposed at an output end of the second linear stepper motor. The blowing and sucking needle device 330 is communicated with the second cylinder, for example, by forward rotation of a second linear stepper motor, so as to drive the second piston rod to extend out of the second cylinder, at this time, the blowing and sucking device 320 pumps air in the blowing and sucking device 430, and the sample in the sample injection device 420 flows into the blowing and sucking device 430 through the second micro flow channel 440. The second linear stepping motor is reversed to drive the second piston rod to retract into the second cylinder, and at the moment, the blowing and sucking device 430 is blown by the blowing and sucking device 320, and the sample in the blowing and sucking device 430 flows into the sample injection device 420 through the second micro flow channel 440. The second piston rod is driven to extend or retract to the second cylinder body through the forward rotation and the reverse rotation of the second linear stepping motor, so that the purpose of exhausting or blowing air to the blowing and sucking device 430 is achieved, samples injected into the sample injection device 420 are blown and evenly mixed back and forth, sample precipitation is prevented, the detected cell concentration is ensured, and the detection precision is improved.
Further, referring to fig. 1 again, the hybrid system 10 further includes a third power source 500 and a driving plate 600, where the suction needle device 230 and the blowing and sucking needle device 330 are disposed on the driving plate 600, and the third power source 500 is used to drive the driving plate 600 to move in a direction approaching or separating from the substrate 410. The third power source 500 may be a motor, and the driving plate 600 is driven to descend by the forward rotation of the motor, the suction needle device 230 is inserted into the suction device 470, and the blowing and sucking needle device 330 is inserted into the blowing and sucking device 430.
In other embodiments, the mixing or staining of the sample in the particle detection unit 400 may be achieved by the suction assembly 200 and the suction device 470 without the provision of the suction assembly 300 and the suction device 430. Optionally, the flow channel has at least two branches that are opened and closed to the suction device 470 by valve control. The valve of one of the branches is opened, the suction assembly 200 pumps or blows air, and the sample flows back and forth between the suction device 470 and the sample injection device 420 through the branch to realize uniform mixing; after mixing, the branch is closed, the valve of the other branch is opened, the color substances can be buried in the other branch, the uniformly mixed sample is pumped by the pumping component 200, and enters the other branch to be dyed and flow to the detection window 460 for detection. Alternatively, the flow channel is a single flow channel, and the suction is applied by the suction assembly 200, so that the sample flows between the suction device 470 and the sample introduction device 420 through the flow channel to achieve uniform mixing or staining. In one embodiment, the flow channel may allow at least a portion of the liquid analyte to flow within the channel, the flow channel having a bend or obstruction at least a portion thereof such that the liquid analyte is at the bend or obstruction for adjusting the liquid analyte flow rate to extend the flow path.
Optionally, the particle detecting unit 400 further includes a color substance disposed in the flow channel, or disposed in the sample injection device 420, or disposed in the suction device 470, or disposed in the blow-suction device 430, and the color substance is used for mixing with the liquid analyte injected into the sample injection device 420. The sample is a liquid analyte.
Adding a sample into the sample injection device 420 through the sample injection port 421, inserting the suction needle-shaped device 230 of the suction assembly 200 into the suction device 470, sucking air from the suction power source 210 of the suction assembly 200 to the suction device 470 through the suction needle-shaped device 230, sucking the sample in the sample injection device 420 into a flow channel of the substrate 410 through the outlet 422, blowing air from the suction power source 210 of the suction assembly 200 to the suction device 470 through the suction needle-shaped device 230, and reversely blowing the sample in the sample injection device 420, wherein the sample can be fully mixed through repeated air suction and air blowing for a plurality of times; in addition, the suction power source 210 of the suction assembly 200 pumps the suction device 470 through the suction needle device 230, and the sample in the sample introduction device 420 is pumped into the flow channel of the substrate 410 through the outlet 422, and finally flows to the detection window 460 for detection. Therefore, the sample is directly injected into the sample injection device 420, and the user does not need to manually mix the sample; the color materials can also be placed in the particle detection unit 400 and in the path in front of the detection window 460, and the sample can be directly mixed with the color materials in the process of pumping and flowing through the pumping device 470 by the pumping assembly 200, so that the step of manually mixing the color materials is omitted, and the detection efficiency is greatly improved.
Referring to fig. 5, an embodiment of the present application provides a mixing method, including the steps of:
in step S110, the liquid analyte is injected into the sample injection device 420 through the sample injection port 421 of the sample injection device 420, wherein the sample injection device 420 is disposed on the substrate 410, a flow channel is disposed in the substrate 410, and a suction device 470 is further disposed on the substrate 410 and is communicated with the sample injection device 420 through the flow channel.
In step S120, the suction needle device 230 is inserted into the suction apparatus 470. For example, the suction needle device 230 may be driven to move in a direction approaching the base plate 410 by the third power source 500 and the driving plate 600 until the suction needle device 230 is inserted into the blowing device 430.
In step S130, the aspirator 220 is driven by the aspiration power source 210 to aspirate the aspiration device 470 through the aspiration needle device 230, so that the liquid analyte in the sample injection device 420 enters the flow channel.
The mixing method can be implemented by using the mixing system 10 in any of the above embodiments, the suction power source 210 of the suction assembly 200 pumps or blows air to the suction device 470 through the suction needle device 230, the sample in the sample feeding device 420 is pumped into the flow channel of the substrate 410 through the outlet 422 or the sample in the flow channel is blown back into the sample feeding device 420, and the sample can be fully mixed through repeated movements. The suction power source 210 of the suction assembly 200 pumps the suction device 470 through the suction needle device 230, and the sample in the sample introduction device 420 is pumped into the flow channel of the substrate 410 through the outlet 422, and finally flows to the detection window 460 for detection. The user does not need to manually mix the samples; the color materials can also be placed in the particle detection unit 400 and in the path in front of the detection window 460, and the sample can be directly mixed with the color materials in the process of pumping and flowing through the pumping device 470 by the pumping assembly 200, so that the step of manually mixing the color materials is omitted, and the detection efficiency is greatly improved.
Referring to fig. 6, an embodiment of the present application further provides a mixing method, including the steps of:
in step S210, the liquid analyte is injected into the sample injection device 420 through the sample injection port 421 of the sample injection device 420, wherein the sample injection device 420 is disposed on the substrate 410, a flow channel is disposed in the substrate 410, and the substrate 410 is further provided with a suction device 470 and a blowing and sucking device 430 respectively communicating with the sample injection device 420 through the flow channel.
In step S220, the suction needle device 330 is inserted into the suction device 430.
In step S230, the blowing and sucking device 320 is driven by the blowing and sucking power source 310 to blow or suck air to the blowing and sucking device 430 through the blowing and sucking needle device 330, so that the liquid analyte in the sample injection device 420 flows into the blowing and sucking device 430 through the flow channel or the liquid analyte in the blowing and sucking device 430 flows into the sample injection device 420 through the flow channel, so that the liquid analytes are uniformly mixed.
In step S240, the suction needle device 230 is inserted into the suction apparatus 470. Step S240 and step S220 may be performed sequentially or simultaneously.
In step S250, the aspirator 220 is driven by the aspiration power source 210 to aspirate the aspiration device 470 through the aspiration needle device 230, so that the mixed liquid analyte in the sample injection device 420 enters the flow channel.
The mixing method can be implemented by using the mixing system 10 in any of the embodiments, the blowing and sucking power source 310 of the blowing and sucking assembly 300 pumps or blows air to the blowing and sucking device 430 through the blowing and sucking needle device 330, the sample in the sample injection device 420 is pumped into the flow channel of the substrate 410 or the sample in the flow channel is reversely blown into the sample injection device 420, and the sample can be fully mixed through repeated and flexible movements; the suction power source 210 of the suction assembly 200 pumps the suction device 470 through the suction needle device 230, and the uniformly mixed sample in the sample introduction device 420 is pumped into the flow channel of the substrate 410, and finally flows to the detection window 460 for detection. The user does not need to mix the sample manually, so that the detection efficiency is greatly improved, the blowing and sucking device 430 pumps or blows air, the sample injected into the sample injection device 420 is blown back and forth to be mixed uniformly, sample precipitation is prevented, the detected cell concentration is ensured, and the detection precision is improved.
Further, in one embodiment, the mixing method further includes the following steps:
in step S260, a color substance is disposed in the flow channel, the sample injection device 420, the aspiration device 470, or the aspiration device 430, and the color substance is mixed with the liquid analyte.
In the path of placing the color materials in the particle detecting unit 400 and before the detecting window 460, the sample is directly mixed with the color materials in the process of pumping the sample by the pumping assembly 200 through the pumping device 470 or pumping and blowing the sample by the blowing and sucking assembly 300 through the blowing and sucking device 430, so that the step of manually mixing the color materials is omitted, the dyeing efficiency is greatly improved, and the detecting efficiency is further improved.
The specific working principle of the above-mentioned mixing system 10 and the mixing method thereof is as follows:
the sample is injected into the sample injection device 420 through the sample injection port 421, and in order to ensure the detected cell concentration, the sample needs to be uniformly mixed before dyeing and detection, so that cell sedimentation is prevented. The third power source 500 drives the driving plate 600 to descend so that the suction needle device 230 is inserted into the suction device 470 and the blowing needle device 330 is inserted into the blowing device 430.
The second linear stepping motor rotates forward to drive the second piston rod to extend out of the second cylinder, and at the moment, the blowing and sucking device 320 pumps air in the blowing and sucking device 430 through the blowing and sucking needle-shaped device 330, and samples in the sample injection device 420 flow into the blowing and sucking device 430 through the second micro-channel 440. Then, the second linear stepping motor is reversed, so that the second piston rod is driven to retract into the second cylinder, at this time, the blowing and sucking device 320 blows air to the blowing and sucking device 430 through the blowing and sucking needle-shaped device 330, and the sample in the blowing and sucking device 430 flows into the sample injection device 420 through the second micro flow channel 440. At least three rounds of aspiration and suction were performed to homogenize the sample.
After the samples are uniformly mixed, the dyeing operation is started, the first piston rod is driven to extend out of the first cylinder body by forward rotation of the first linear stepping motor, at the moment, the aspirator 220 pumps air in the suction device 470 through the suction needle-shaped device 230, the samples are pumped into the first micro-flow channel 450 through the outlet 422, the sample dyeing is realized in the flowing process of the first micro-flow channel 450, and finally, the samples flow into the detection window 460, and finally, the samples reach the filter 490. Because the filter 490 is made of a hydrophobic and air-permeable material, after the liquid in the first micro flow channel 450 reaches the filter 490, the liquid in the whole first micro flow channel 450 is kept in a non-flowing state or a slow flowing state by the filter 490, so that the liquid in the detection window 460 is ensured to be non-flowing or slow flowing, and the observation is facilitated.
Therefore, the user does not need to dye manually, the sample can be directly injected into the sample injection device 420, the injection amount of the sample has no influence on the accuracy of the result, the color substance embedded in the first micro flow channel 450 is pre-embedded, the step of manually preparing the color substance is omitted, the consistency of the dye concentration is ensured, the accuracy is improved, the sampling amount accurately controlled by the suction power source 210 and the aspirator 220 is ensured, the full-automatic detection process is ensured, the result is more accurate, and the interference of manual operation is avoided. In addition, the sample is uniformly mixed in a blowing and sucking mode before the dyeing operation, so that cell sedimentation is prevented, the detected cell concentration is ensured, and the detection precision is improved.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the application, which are described in detail and are not to be construed as limiting the scope of the application. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application. Accordingly, the scope of protection of the present application is to be determined by the appended claims.
In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present application and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present application, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.
In the present application, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.
In the present application, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.
Claims (14)
1. A particle detection unit, characterized in that the particle detection unit comprises:
a substrate;
the sample injection device is arranged on the substrate and is provided with a sample injection port and an outlet;
the flow channel is embedded in the substrate, and one end of the flow channel is communicated with the sample injection device through the outlet;
the suction device is arranged on the substrate, is connected with the sample introduction device through a flow channel, comprises a filter, a suction cavity and a sealing plug, wherein a hydrophobic and breathable material is arranged in the filter, the filter is arranged at the bottom of the suction cavity, the suction cavity is communicated with the flow channel through the filter, and the sealing plug is arranged on the suction cavity and is used for being connected with a suction assembly for providing suction power;
the detection window is arranged on the substrate, one end of the detection window is communicated with the flow channel, the other end of the detection window is communicated with the suction device through a connecting channel, and a humidity-sensitive detection piece is arranged in the connecting channel;
the blowing and sucking device is arranged on the substrate and is provided with a blowing and sucking mixing port, and the sample injection device is provided with a sample injection mixing port which is mutually spaced with the outlet; one end of one flow channel is communicated with the blowing and sucking device through the blowing and sucking mixing port, and the other end is communicated with the sample injection device through the sample injection mixing port.
2. The particle detection unit of claim 1, wherein the flow channel is configured to allow at least a portion of the liquid analyte to flow within the channel, the flow channel having a bend or obstruction such that the liquid analyte is at the bend or obstruction for adjusting a liquid analyte flow rate to extend the flow path.
3. The particle detection unit of claim 1, wherein the flow channel has at least two branches, the two branches being in communication with and out of communication with the suction device by valve control.
4. A particle detection unit as claimed in any one of claims 1 to 3 wherein the substrate is a sheet material which can encapsulate at least a portion of the flow channel.
5. The particle detection unit of claim 4, wherein the flow channel has a flow channel flux equivalent diameter in the range of 10-1000 μm.
6. The particle detection unit of claim 1, further comprising a color substance disposed within the flow channel, or within the sample injection device, or within the aspiration device, or within the blow-aspiration device, the color substance configured to mix with a liquid analyte injected into the sample injection device.
7. A mixing system, comprising:
a particle detection unit according to any one of claims 1 to 6; a kind of electronic device with high-pressure air-conditioning system
The suction assembly comprises a suction power source, a suction device and a suction needle-shaped device, wherein the suction needle-shaped device is used for being inserted into the suction device and communicated with the suction device, and the suction power source is used for driving the suction device to act so as to suck air into the suction device through the suction needle-shaped device.
8. The mixing system of claim 7, wherein the suction power source is a first linear stepper motor, the aspirator includes a first cylinder and a first piston rod, one end of the first piston rod is disposed in the first cylinder, the other end of the first piston rod is disposed at an output end of the first linear stepper motor, and the suction needle device is in communication with the first cylinder.
9. The mixing system of claim 7, further comprising a blow-and-suction assembly comprising a blow-and-suction power source, a blow-and-suction device, and a blow-and-suction needle device, wherein the blow-and-suction needle device is configured to be inserted into a blow-and-suction device, wherein the blow-and-suction needle device is in communication with the blow-and-suction device, and wherein the blow-and-suction power source is configured to drive the blow-and-suction device to blow or suck air through the blow-and-suction needle device.
10. The mixing system of claim 9, wherein the blowing and sucking power source is a second linear stepper motor, the blowing and sucking device comprises a second cylinder body and a second piston rod, one end of the second piston rod is arranged in the second cylinder body, and the other end of the second piston rod is arranged at the output end of the second linear stepper motor; the blowing and sucking needle-shaped device is communicated with the second cylinder body.
11. The mixing system of claim 10, further comprising a third power source and a drive plate, wherein the suction needle and the blowing needle are disposed on the drive plate, and wherein the third power source is configured to drive the drive plate to move in a direction toward or away from the substrate.
12. A mixing method, characterized in that it is mixed by the mixing system according to claim 7, comprising the steps of:
injecting liquid analyte into the sample injection device through a sample injection port of the sample injection device, wherein the sample injection device is arranged on a substrate, a flow channel is arranged in the substrate, and a suction device communicated with the sample injection device through the flow channel is also arranged on the substrate;
inserting a suction needle device into the suction device;
the suction device is sucked through the suction needle-shaped device by driving the suction device to act through the suction power source, so that liquid analytes in the sample injection device enter the flow channel.
13. A mixing method, characterized in that it is mixed by the mixing system according to claim 7, comprising the steps of:
injecting liquid analyte into the sample injection device through a sample injection port of the sample injection device, wherein the sample injection device is arranged on a substrate, a flow channel is arranged in the substrate, and a suction device and a blowing and sucking device which are respectively communicated with the sample injection device through the flow channel are also arranged on the substrate;
inserting the blowing and sucking needle-shaped device into the blowing and sucking device;
the blowing and sucking device is driven by the blowing and sucking power source to act so as to blow or suck air to the blowing and sucking device through the blowing and sucking needle-shaped device, so that liquid analytes in the sample injection device flow into the blowing and sucking device through the flow channel or flow into the sample injection device through the flow channel, and the liquid analytes are uniformly mixed;
inserting a suction needle device into the suction device;
the aspirator is driven by the aspiration power source to act so as to aspirate the aspiration device through the aspiration needle-shaped device, so that the evenly mixed liquid analyte in the sample injection device enters the flow channel.
14. The mixing method according to claim 13, comprising the steps of:
and a color substance is arranged in the flow channel, the sample injection device, the suction device or the blowing and sucking device, and the color substance is mixed with the liquid analyte.
Priority Applications (2)
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| CN202111236637.7A CN114018787B (en) | 2021-10-23 | 2021-10-23 | Particle detection unit, mixing system and mixing method |
| PCT/CN2022/126795 WO2023066388A1 (en) | 2021-10-23 | 2022-10-21 | Particle detection unit, detection plate and mixing method |
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| CN202111236637.7A CN114018787B (en) | 2021-10-23 | 2021-10-23 | Particle detection unit, mixing system and mixing method |
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| CN114018787B (en) * | 2021-10-23 | 2023-10-20 | 广州市艾贝泰生物科技有限公司 | Particle detection unit, mixing system and mixing method |
| CN116355725B (en) * | 2023-03-07 | 2024-04-05 | 广州市艾贝泰生物科技有限公司 | Distributor, distributing device and distributing method |
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| WO2023066388A1 (en) | 2023-04-27 |
| CN114018787A (en) | 2022-02-08 |
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