CN105381827A - Microfluidic chip magnetic bead even mixing apparatus and method - Google Patents
Microfluidic chip magnetic bead even mixing apparatus and method Download PDFInfo
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- CN105381827A CN105381827A CN201510954760.0A CN201510954760A CN105381827A CN 105381827 A CN105381827 A CN 105381827A CN 201510954760 A CN201510954760 A CN 201510954760A CN 105381827 A CN105381827 A CN 105381827A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
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Abstract
The present invention provides a microfluidic chip magnetic bead even mixing apparatus and a method, the microfluidic chip magnetic bead even mixing apparatus comprises a first container, a second container, a base, a gas intake hole, a groove and a diaphragm; the base includes a first surface, a second surface, a first through hole and a second through hole, the surface, near the base, of the first container is provided with a first opening, the shape of the first through hole is consistent with the shape of the first opening, the surface, near the base, of the second container is provided with a second opening, the shape of the second through hole is consistent with the shape of the second opening, the groove is arranged on the second surface of the base, and located in the center of the base, the base is provided with a first channel communicating the groove with the first through hole, a second channel communicating the groove with the second through hole, and a third channel communicating the gas intake hole and the first through hole, and the diaphragm covers the groove, first through hole, the second through hole and the second surface of the base. The microfluidic chip magnetic bead even mixing apparatus can achieve rapid even mixing of magnetic beads in a smaller space.
Description
Technical field
The present invention relates to micro-fluidic chip field, be specifically related to a kind of micro-fluidic chip magnetic bead evenly mixing device and method.
Background technology
Magnetic bead mixing is a physical process, and in micro-fluidic chip, carry out magnetic bead reaction mixing is required step, and its Blending Efficiency of Blending depends on the apparatus and method of use.Micro-fluidic chip, as carrier that is chemical and biological respinse, is widely applied in these areas.More mature technology is by wherein integrated, the high sensitivity contribution of magnetic bead technology to analytic process has become chemistry and the requisite process of biological respinse, but along with the increase of functional module, integration degree is also more and more higher, fluid Space Day convergent is in the chips little, the microfluidic laboratory that tradition needs independent a few step to complete, completes on a single die according to test needs are integrated now.Tradition relies on liquid flow movement to promote the blending manner of diffusion, also cannot meet the requirement that magnetic bead rapidly and efficiently mixes in micro-fluidic chip.
At present, the micro-fluidic chip evenly mixing device of extensive use is mainly divided into passive mixing and initiatively mixes two classes.The natural diffuseness that the power that passive mixing mainly relies on liquid stream self is formed realizes mixing, such as: oscillator mixing etc., cannot meet integrated needs; Initiatively mixing adopts active magnet adsorption mode, and the main change in location relying on external magnets magnetic force guides magnetic bead blending process.This mode can reach more satisfactory Blending Efficiency of Blending, and magnetic bead motion can be controlled, but active electrical magnet volume is larger, and need at multiple location arrangements magnet, take large quantity space, be unfavorable for integrated, the realization of chip, in addition because the mobile of magnetic bead needs the magnetic force by adjusting different azimuth to realize, require a great deal of time.
Summary of the invention
The invention provides a kind of micro-fluidic chip magnetic bead evenly mixing device and method, realize the quick mixing of magnetic bead in magnetic bead solution in less space.
First aspect, the present invention proposes a kind of micro-fluidic chip magnetic bead evenly mixing device, comprising: the first container, second container, base, air admission hole, groove and diaphragm;
Described base comprises first surface, second surface and the first through hole, the second through hole, and described first container is provided with the first opening near the surface of described base, and described first through hole is consistent with described first opening shape; Described second container is provided with the second opening near the surface of described base, and described second through hole is consistent with described second opening shape; The second surface that described groove is arranged on described base is positioned at the center of described base, described base is provided with the first passage being communicated with described groove and described first through hole, the second channel being communicated with described groove and described second through hole, is communicated with the third channel of described air admission hole and described first through hole; The second surface of groove, the first through hole, the second through hole and base described in described membrane covered.
Preferably, described device also comprises permanent magnet.
Preferably, described device also comprises the filter membrane of permeable watertight, and described filter membrane is arranged in described third channel.
Preferably, described device also comprises purge valve, and described purge valve is arranged on the position that described third channel is connected with described first through hole, for controlling opening and closedown of described third channel.
Preferably, described device also comprises described in cleaning liquid pool and cleans between liquid pool and described base and be provided with third through-hole.
Preferably, described device also comprises waste liquid pool, is provided with fourth hole between described waste liquid pool and described base.
Preferably, dividing plate is provided with between described cleaning liquid pool and described waste liquid pool.
Preferably, described device comprises one or more second container.
Second aspect, present invention also offers a kind of micro-fluidic chip magnetic bead mixing method, comprising:
Solution in second container is after the second through hole and second channel flow into groove, and the diaphragm covered on the second through hole receives positive air pressure, and diaphragm is heaved, blocking second channel;
The diaphragm covered in a groove receives positive air pressure, and force the solution in groove to flow into after the first container through first passage, diaphragm is heaved, blocking first passage;
In the first container, malleation air-flow is introduced, to mix the magnetic bead solution in described first container according to described malleation air-flow by air admission hole and third channel.
Preferably, after introducing malleation air-flow by air admission hole and third channel in the first container, described method also comprises:
By changing the distance between permanent magnet and the first container, assembling and breaing up the magnetic bead in the solution of described first container.
The invention provides a kind of micro-fluidic chip magnetic bead evenly mixing device and method, solution is flow in groove by second container, closedown second channel is heaved by diaphragm, the solution in groove is forced to flow into the first container by heaving of diaphragm under groove, at the first container after above-mentioned steps contains magnetic bead solution and sample solution, malleation air-flow is passed into air admission hole, by controlling pressure and the flow of malleation air-flow, promote the rapid movement of magnetic bead in the first container solution, realize the solution of magnetic bead chain in reaction cup constantly to mix and diffusion motion, thus in less space, realize the quick mixing of magnetic bead in magnetic bead solution.
Accompanying drawing explanation
The micro-fluidic chip magnetic bead evenly mixing device schematic diagram that Fig. 1 provides for one embodiment of the invention;
The sectional view in A-A ' direction in Fig. 1 that Fig. 2 provides for one embodiment of the invention;
Fig. 3 shows the micro-fluidic chip magnetic bead evenly mixing device schematic diagram for another embodiment of the present invention provides;
Fig. 4 is pneumatic mixing oscillogram in one embodiment of the invention;
Fig. 5 is Pneumatic cleaning oscillogram in one embodiment of the invention;
Description of reference numerals:
1, liquid pool is cleaned; 2, sample cup; 3, cup is marked; 4, substrate cup; 5, wrap by cup; 6, magnetic bead cup; 7, waste liquid pool; 8, groove; 9, reaction cup; 10, the first through hole; 11, air admission hole; 12, purge valve; 13, the second through hole; 14, diaphragm; 15, permanent magnet; 16, base; 17, first passage; 18, second channel; 19, third channel.
Detailed description of the invention
For making the object of the embodiment of the present invention, technical scheme and advantage clearly, below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly described, obviously, described embodiment is the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
The invention provides a kind of micro-fluidic chip magnetic bead evenly mixing device, will the magnetic bead solution of magnetic bead be had, be sent in reaction cup from magnetic bead cup by diaphragm cup and the cooperation of main membrane pump by main membrane pump; Include the sample solution of sample simultaneously, be also sent in reaction cup by diaphragm cup and the cooperation of main membrane pump from sample cup by main membrane pump; At this moment two kinds of solution enter in reaction cup simultaneously.Positive pressure gas flows through air admission hole purge valve and is connected in reaction cup, by to the pressure of outside positive pressure gas stream and the program control adjustment of flow, air-flow promotes magnetic bead liquid rapid movement by reaction cup bottom sides in a pulsing mode, realizes making constantly mixing and diffusion motion in the solution of magnetic bead chain in reaction cup.At this moment the permanent magnet near driving reaction cup, near magnetic bead solution, can adsorb in the reaction cup of magnetic bead chain near permanent magnet and assemble; Drive the permanent magnet near reaction cup to leave reaction cup, then can re-start mixing and the diffusion motion of magnetic bead chain.Under the promotion of air flow power, in micro-fluidic solution, can ensure that solution rapid mixing complete from the number of times gathering diffusion by repeating magnetic bead chain.
Fig. 1 and Fig. 2 shows the micro-fluidic chip magnetic bead evenly mixing device schematic diagram that one embodiment of the invention provides, as depicted in figs. 1 and 2, micro-fluidic chip magnetic bead evenly mixing device comprises: sample cup 2, mark cup 3, substrate cup 4, colour developing cup 5, magnetic bead cup 6, reaction cup 9, base 16, air admission hole 11, groove 8 and diaphragm 14, base 16 comprises first surface, second surface and the first through hole, second through hole, reaction cup 9 is provided with the first opening near the surface of base 16, first through hole and described first opening shape are consistent, specimen cup 2 is provided with the second opening near the surface of base 16, second through hole is consistent with described second opening shape, the second surface that groove 8 is arranged on base 16 is positioned at the center of base 16, base 16 is provided with the second channel of the first passage of communication groove 8 and the first through hole, communication groove 8 and the second through hole, be communicated with the third channel of air admission hole 11 and the first through hole, diaphragm 14 covers the second surface of groove 8, first through hole, the second through hole and base 16, mark cup 3, substrate cup 4, colour developing cup 5, magnetic bead cup 6 structure and specimen cup 2 similar,
Be understandable that, here reaction cup 9 is the first container, specimen cup 2, mark cup 3, substrate cup 4, colour developing cup 5, magnetic bead cup 6 are second container, second container in micro-fluidic chip magnetic bead evenly mixing device provided by the invention can expand to multiple according to actual needs, the various reactions relevant with magnetic bead can be realized in less space, in order to better illustrate, in the present embodiment, second container is 5, but the present invention is not limited to only comprise 5 second containers, in actual application, can expand accordingly and reduce.
In above-described embodiment, reaction cup 9 is the container of upper and lower opening, and the opening shape of shape of through holes corresponding with reaction cup 9 on base 16 and reaction cup lower surface is consistent, coincide to keep the lower surface of the through hole on base 16 and reaction cup 9; Specimen cup 2 is upper surface open, the container that lower surface is closed, and be provided with a hole in the center of lower surface, the shape of shape of through holes corresponding with specimen cup 2 on base 16 and specimen cup 2 lower surface centre bore is consistent, same, shape of through holes corresponding with mark cup 3, substrate cup 4, the cup 5 that develop the color, magnetic bead cup 6 on base with mark cup 3, substrate cup 4, the cup 5 that develops the color, magnetic bead cup 6 lower surface centre bore shape consistent.
In addition, micro-fluidic chip magnetic bead evenly mixing device provided by the invention also comprises permanent magnet 15, and permanent magnet 15 is arranged on described reaction cup 9 around, and the position of permanent magnet 15 can be moved within the scope of predeterminable range.
In third channel, micro-fluidic magnetic bead evenly mixing device also comprises the filter membrane 10 of permeable watertight.On the position that third channel is connected with the first through hole, micro-fluidic magnetic bead evenly mixing device also comprises purge valve, for controlling opening and closedown of described third channel.
What deserves to be explained is, micro-fluidic magnetic bead evenly mixing device also comprises cleaning liquid pool 1, is provided with third through-hole between cleaning liquid pool 1 and base 16, this device also comprises waste liquid pool 7, be provided with fourth hole between waste liquid pool 7 and base 16, meanwhile, between cleaning liquid pool 1 and described waste liquid pool 7, be provided with dividing plate.
Fig. 3 shows the micro-fluidic chip magnetic bead evenly mixing device schematic diagram that another embodiment of the present invention provides, and as shown in Figure 3, the position of permanent magnet 15 can be moved around reaction cup 9.
A kind of micro-fluidic chip magnetic bead evenly mixing device provided by the invention, adopt permanent-magnet, assemble magnetic bead to provide without the need to power, and permanent magnet is cheap, alternative strong, energy-conservation, and structure is simple, solve the circuit control system of source magnet complexity, simplify the complicated not easily integrated difficult problem of volume; In addition, micro-fluidic chip magnetic bead evenly mixing device provided by the invention is with pneumatic mode active drive magnetic bead, the flowing of air stream drives magnetic bead solution is controlled by the switch matching programization of gas flow adjustment and purge valve, improve solution blending efficiency, solve existing apparatus length consuming time, and a difficult problem for magnetic bead static sedimentation.Volume of the present invention is little, by micro-fluidic membrane pump, purge valve, Miniature permanent magnet body being combined in the Integration Design on micro-fluidic chip, several functions can be realized in 2x4cm areal extent, micro-fluidic chip magnetic bead evenly mixing device provided by the invention can to micro-fluidic magnetic bead solution realize multiple processing capacity as magnetic bead mix fast, shift, gather, rupture, clean, waste discharge, not only can improve blending efficiency when for mixing, the method for compatibility test simultaneously can realize complete biochemical test.And the present invention has portability, the miniflow membrane pump of this structure, permanent magnet, purge valve, function cup fluid channel can need change membrane pump size according to test on micro-fluidic chip, liquids and gases runner latus rectum, permanent magnet flux can also need according to test the quantity increasing application cup, slightly adjusts and just can be applicable in different test occasions.It is simple that above-mentioned micro-fluidic magnetic bead evenly mixing device can make magnetic bead mix structure fast, good mixing effect, volume compact, integrability.
In addition, the invention provides a kind of micro-fluidic chip magnetic bead mixing method, comprising:
Solution in second container is after the second through hole 13 and second channel 18 flow into groove 8, and the diaphragm 14 covered on the second through hole 13 receives positive air pressure, and diaphragm 14 is heaved, blocking second channel 13;
The diaphragm 14 covered in groove 8 receives positive air pressure, and force the solution in groove 8 to flow into after the first container through first passage 17, diaphragm 14 is heaved, blocking first passage 17;
In the first container, malleation air-flow is introduced, to mix the magnetic bead solution in described first container according to described malleation air-flow by air admission hole 11 and third channel 19.
After introducing malleation air-flow by air admission hole 11 and third channel 19 in the first container, described method also comprises:
By changing the distance between permanent magnet 15 and the first container, assembling and breaing up the magnetic bead in the solution of described first container.
After air admission hole 11 receives malleation air-flow after third channel enters the first container, described method also comprises:
Permanent magnet 15, by changing the distance between the first container, is assembled and breaks up the magnetic bead in the solution of described first container.
Concrete, the invention provides a kind of micro-fluidic magnetic bead mixing method, the detailed process of the method is described below:
(1) microfluid sample solution is by sample cup 2 after the second through hole and second channel flow into groove 8, and pass into positive air pressure by membrane pump to the diaphragm covered on the second through hole, diaphragm is heaved, blocking second channel;
Want that the diaphragm covered in groove 8 passes into positive air pressure by main membrane pump, force the sample solution in groove 8 to flow into reaction cup 9 through first passage, under the effect of positive air pressure, block first passage with rear film;
Same, the magnetic bead solution in magnetic bead cup 6 also enters in reaction cup 9 by the way; Each magnetic bead in magnetic bead solution is with antibody, and magnetic bead can have different-diameter, and microfluid solution medium can be different according to application.
Two kinds of solution form mixed solution in reaction cup;
(2) enter reaction cup 9 through third channel after air admission hole 11 receives malleation air-flow, mix under the effect of malleation air-flow to make the liquid in reaction cup 9.
Be understandable that, enter malleation air-flow from air admission hole 11, control purge valve 12 in a pulsing mode, thus rhythmical opening and closing third channel, magnetic bead mixed solution in malleation air-flow driving a reaction cup is not stopped transport dynamic, promotes the formation of magnetic bead chain, and mixes fast;
Be understandable that, have and can introduce outside gas circuit air admission hole 11 and can the purge valve 12 of pneumatic channel of switch control rule, bottom reaction cup 9, air-flow is introduced from cup bottom side to the motion of promotion magnetic bead solution rotating in tangentially position, air admission hole 11 is connected to external pressure source, filter membrane 10 position is provided with air-permeable, hydrophobic filter membrane, can filtered air, the diameter of adjustment air flue can realize flow-control, and the switch of control purge valve 12 can control purge valve 12 switch time and interval can realize the magnetic bead solution mixing speed of action and the control of frequency.The self-defined control of flow, the frequency, pressure can be realized by programme-control, realize the mixing of magnetic bead solution; Preferably, the gas flow scope entered in reaction cup 9 is (0.05 ~ 240mL/min), and pressure limit is (0.01 ~ 0.5MPa), and in micro-fluidic air admission hole 11, air flue areal extent is (0.25 ~ 4mm2).
(3) permanent magnet 15 is by changing the distance between reaction cup 9, assembles and breaks up the magnetic bead in the solution in described reaction cup 9.
Be understandable that, above move bottom permanent magnet 15 to reaction cup 9, magnetic bead is assembled near permanent magnet; There is the adjustable permanent magnet in far and near position 15, drive permanent magnet 15 to change the far and near distance of distance magnetic bead solution by minitype cylinder, the congregational rate of magnetic bead in magnetic bead solution can be changed; Permanent magnet 15, by changing different magnet material and volume, is carried out the change of magnetic field intensity, can be placed on and have the arbitrarily angled of magnetomechanical effects or position during use.Permanent magnet 15 can axial stretching action, and the Distance geometry magneticaction of distance reaction cup 9 be that proportionate relationship changes.
What deserves to be explained is, above-mentioned 3 kinds of modes are capable of being combined to be used or single use.
(4) in experimentation, can, by controlling purge valve 12 and intensified pressure pulsation rhythm, magnetic bead chain be ruptured; Label solution in mark cup 3 can be imported in reaction cup 9 and mark; Substrate solution in substrate cup 4 can be imported in reaction cup 9 and develop the color; By the discharging of waste liquid after separation magnetic bead in waste liquid pool 7, can import cleaning fluid from cleaning liquid pool 1 and clean; Mark cup and substrate cup, the process that mark cup 3 and the solution in substrate cup 4 import reaction cup 9 is consistent with the process of specimen cup 2, does not repeat them here.
(5) mixing of magnetic bead solution complete after, judge whether mixing meets the requirements, after above-mentioned 4 steps of sequencing complete, permanent magnet 15 can be left reaction cup, closedown purge valve 12 and main membrane pump.Nitrite ion in colour developing cup 5 is detected.
Come better to be described a kind of micro-fluidic chip magnetic bead mixing method provided by the invention below by a concrete experimental technique:
Chemiluminescence double antibody sandwich method magnetic bead mixes reaction test fast:
(1) the magnetic bead cup 6 of magnetic bead solution is added: the immunomagnetic beads (comprising amino magnetic bead, carboxyl magnetic bead) of antibody coupling, magnetic bead particle diameter (1um-0.2 μm), liquor capacity can at (20-50ul).
(2) sample solution is added to specimen cup 2:100-400 μ l sample.
(3) pneumatic mixing in reaction cup: time 8-10min (pneumatic mixing waveform is shown in Fig. 4)
(4) wash: wash three times.After permanent magnet holds magnetic bead, assemble completely until magnetic bead, with miniflow membrane pump, waste liquid is entered waste liquid pool, add cleaning fluid Pneumatic cleaning.(Pneumatic cleaning waveform is shown in Fig. 5)
(5) add the antibody-solutions of enzyme labeling: 100-200 μ l enzyme labelled antibody, from mark cup, add enzyme mark resist in reacting hole.
(6) pneumatic mixing reaction:
(7) wash: wash five times.After permanent magnet holds magnetic bead, assemble completely until magnetic bead, with miniflow membrane pump, waste liquid is entered waste liquid pool, add cleaning fluid Pneumatic cleaning.
(8) substrate is added: 100-200 μ l substrate A PS-5, enters reacting hole from substrate hole.
(9) pneumatic mixing: bleed pressure: 10kpa, pneumatic time: 30S.
(10) reading numerical values: after equilibrium at room temperature reaction 2min, reading numerical values.
Wherein, the liquor capacity of description can change according to practical application.
The bright micro-fluidic chip magnetic bead mixing method provided of this law, by adopting membrane pump technology on micro-fluidic chip, realizes the transmission of micro-fluidic magnetic bead solution, mixing, cleaning, waste discharge, magnetic bead aggregation capability; There is provided mixing power by air-flow, speed and the efficiency of mixing can be improved; The effect of assembling magnetic bead is realized by passive permanent magnet body.
It will be understood by those skilled in the art that adaptively to change the module in the equipment in embodiment and they are arranged and be in one or more equipment that this embodiment is different.Module in embodiment or unit or assembly can be combined into a module or unit or assembly, and multiple submodule or subelement or sub-component can be put them in addition.Except at least some in such feature and/or process or unit is mutually exclusive part, any combination can be adopted to combine all processes of all features disclosed in this description (comprising adjoint claim, summary and accompanying drawing) and so disclosed any method or equipment or unit.Unless expressly stated otherwise, each feature disclosed in this description (comprising adjoint claim, summary and accompanying drawing) can by providing identical, alternative features that is equivalent or similar object replaces.
In addition, those skilled in the art can understand, although embodiments more described herein to comprise in other embodiment some included feature instead of further feature, the combination of the feature of different embodiment means and to be within scope of the present invention and to form different embodiments.Such as, in the following claims, the one of any of embodiment required for protection can use with arbitrary combination.
All parts embodiment of the present invention with hardware implementing, or can realize with the software module run on one or more processor, or realizes with their combination.The present invention will be described instead of limit the invention to it should be noted above-described embodiment, and those skilled in the art can design alternative embodiment when not departing from the scope of claims.In the claims, any reference symbol between bracket should be configured to limitations on claims.Word " comprises " not to be got rid of existence and does not arrange element in the claims or step.Word "a" or "an" before being positioned at element is not got rid of and be there is multiple such element.The present invention can by means of including the hardware of some different elements and realizing by means of the computer of suitably programming.In the unit claim listing some devices, several in these devices can be carry out imbody by same hardware branch.Word first, second and third-class use do not represent any order.Can be title by these word explanations.
Last it is noted that one of ordinary skill in the art will appreciate that: above each embodiment, only in order to technical scheme of the present invention to be described, is not intended to limit; Although with reference to foregoing embodiments to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein some or all of technical characteristic; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the scope of the claims in the present invention.
Claims (10)
1. a micro-fluidic chip magnetic bead evenly mixing device, is characterized in that, comprising: the first container, second container, base, air admission hole, groove and diaphragm;
Described base comprises first surface, second surface and the first through hole, the second through hole, and described first container is provided with the first opening near the surface of described base, and described first through hole is consistent with described first opening shape; Described second container is provided with the second opening near the surface of described base, and described second through hole is consistent with described second opening shape; The second surface that described groove is arranged on described base is positioned at the center of described base, described base is provided with the first passage being communicated with described groove and described first through hole, the second channel being communicated with described groove and described second through hole, is communicated with the third channel of described air admission hole and described first through hole; The second surface of groove, the first through hole, the second through hole and base described in described membrane covered.
2. device as claimed in claim 1, it is characterized in that, described device also comprises permanent magnet.
3. device as claimed in claim 2, it is characterized in that, described device also comprises the filter membrane of permeable watertight, and described filter membrane is arranged in described third channel.
4. device as claimed in claim 3, it is characterized in that, described device also comprises purge valve, and described purge valve is arranged on the position that described third channel is connected with described first through hole, for controlling opening and closedown of described third channel.
5. device as claimed in claim 4, it is characterized in that, described device also comprises cleaning liquid pool, is provided with third through-hole between described cleaning liquid pool and described base.
6. device as claimed in claim 5, it is characterized in that, described device also comprises waste liquid pool, is provided with fourth hole between described waste liquid pool and described base.
7. device as claimed in claim 6, is characterized in that, be provided with dividing plate between described cleaning liquid pool and described waste liquid pool.
8. the device as described in any one of claim 7, is characterized in that, described device comprises one or more second container.
9. a kind of micro-fluidic chip magnetic bead mixing method of a kind of micro-fluidic chip magnetic bead evenly mixing device of an application rights requirement according to any one of 1-8, is characterized in that, comprising:
Solution in second container is after the second through hole and second channel flow into groove, and the diaphragm covered on the second through hole receives positive air pressure, and diaphragm is heaved, blocking second channel;
The diaphragm covered in a groove receives positive air pressure, and force the solution in groove to flow into after the first container through first passage, diaphragm is heaved, blocking first passage;
In the first container, malleation air-flow is introduced, to mix the magnetic bead solution in described first container according to described malleation air-flow by air admission hole and third channel.
10. method as claimed in claim 9, it is characterized in that, after introducing malleation air-flow by air admission hole and third channel in the first container, described method also comprises:
By changing the distance between permanent magnet and the first container, assembling and breaing up the magnetic bead in the solution of described first container.
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| CN109718873A (en) * | 2017-10-31 | 2019-05-07 | 中国科学院大连化学物理研究所 | The polynary immune response system of micro- magnetic bead based on digital drop micro-fluidic chip |
| CN112881235A (en) * | 2019-11-29 | 2021-06-01 | 深圳市帝迈生物技术有限公司 | Sample analysis device and method for calculating solidification time |
| CN112899141A (en) * | 2021-02-01 | 2021-06-04 | 海南微氪生物科技股份有限公司 | Automatic nucleic acid extraction detector and detection method thereof |
| CN114018787A (en) * | 2021-10-23 | 2022-02-08 | 广州市艾贝泰生物科技有限公司 | Particle detection unit, mixing system and mixing method |
| CN115684021A (en) * | 2022-11-15 | 2023-02-03 | 苏州思迈德生物科技有限公司 | Microfluidic chip for turbidimetry detection and detection device |
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| CN114018787A (en) * | 2021-10-23 | 2022-02-08 | 广州市艾贝泰生物科技有限公司 | Particle detection unit, mixing system and mixing method |
| CN114018787B (en) * | 2021-10-23 | 2023-10-20 | 广州市艾贝泰生物科技有限公司 | Particle detection unit, mixing system and mixing method |
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