CN109682962A - Immunofluorescence test system and detection method based on micro-fluidic chip - Google Patents
Immunofluorescence test system and detection method based on micro-fluidic chip Download PDFInfo
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- CN109682962A CN109682962A CN201910030928.7A CN201910030928A CN109682962A CN 109682962 A CN109682962 A CN 109682962A CN 201910030928 A CN201910030928 A CN 201910030928A CN 109682962 A CN109682962 A CN 109682962A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
- B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
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Abstract
The invention discloses a kind of Immunofluorescence test system and detection method based on micro-fluidic chip, including chip body, one or more is formed with inside it independently of one another for testing the test runner of sample to be tested, test runner includes reaction tank and the first feed pathway being connected to reaction tank, the second feed pathway and liquid outlet channel.Wherein, the height of liquid outlet channel is respectively less than the diameter of the microballoon.First feed pathway is used to be passed through the microspheres solution and detection antibody of capture antibody modification for being passed through sample solution, the second feed pathway.The height of liquid outlet channel is respectively less than the diameter of microballoon in the present invention, so microballoon can only flow in the second feed pathway, and it rests in reaction tank and is reacted with subsequent sample and reaction reagent, without being sucked out out of liquid outlet channel, to eliminate the special constructions such as micro- weir or micro-valve, keep chip structure simpler, and be easy to make, cost is lower.
Description
Technical field
The invention belongs to microfluidic art more particularly to a kind of Immunofluorescence test systems based on micro-fluidic chip
And detection method.
Background technique
Real-time test (POCT) system is that one kind can realize the analysis method quickly detected in the lab.POCT equipment
Useful diagnostic message can be provided for patient with severe symptoms, and POCT equipment has been applied to clinically, such as gestation detection, urine
Sugar and ketone detection etc..For example, HCG test card and HBV test card may be implemented quickly to detect.However, traditional laboratory testing
Complicated equipment, complicated process and longer detection time are needed, nursing properly and timely cannot be provided for patient.Cause
This, it is necessary to develop it is a kind of low cost and portable device, with improve monitoring efficiency of the biomarker in biochemical diagnosis and
Accuracy.In the past few years, micro-total analysis system (μ-TAS) is vigorously sent out due to its Miniaturization Design in life science
Exhibition.Basic operation (including sample preparation, reaction, separation and detection) can be integrated into several square centimeters of chip by the system,
To carry out automatic biochemical and medical analysis, and many microfluid mancarried devices have been developed and have been examined for biochemistry detection and medicine
It is disconnected.Wherein, immunoassay has become most important analysis method in clinical detection and Biochemical Research based on its high specificity
One of.It is used as the standard technique for detecting various analytes as the conventional determining method of representative using ELISA, this traditional being immunized divides
Analysis usually carries out in 96 orifice plates, has the shortcomings that reagent consumption is mostly long with detection time.And traditional microfluidic immunological technique
The special constructions such as micro- weir and micro-valve must be used when making solid phase carrier using non-magnetic microspheres.These problems may be prevented finally
Immunoassay becomes practical technique and care diagnostic tool in business application,
Summary of the invention
First technical problem to be solved by this invention be to provide a kind of structure it is simple, can to avoid use micro- weir or
The Immunofluorescence test system of the special constructions such as micro-valve.
Second technical problem to be solved by this invention is to provide a kind of inspection using above-mentioned micro-fluidic chip system
Survey method.
In order to solve the first technical problem mentioned above, the present invention adopts the following technical scheme:
A kind of Immunofluorescence test system based on micro-fluidic chip, including chip body, inside be formed with one or
The a plurality of test runner for being used to test sample to be tested independently of one another；
The test runner includes reaction tank and the first feed pathway being connected to the reaction tank, the second feed pathway
And liquid outlet channel；
First feed pathway is for being passed through sample solution, and second feed pathway is for being passed through capture antibody modification
Microspheres solution and detection antibody, the height of the liquid outlet channel is respectively less than the diameter of the microballoon.
Further, the chip body includes upper substrate, middle partition and the lower substrate for being sequentially overlapped and sealing cooperation；Its
In,
It is logical that the end face that the upper substrate is in contact with the middle partition is recessed first feed liquor being sequentially connected to
Road, first chamber and the liquid outlet channel；
The end face that the lower substrate is in contact with the middle partition is recessed described second be sequentially connected to, and liquid is logical out
Road and second chamber；
The first chamber and second chamber by penetrate through the through-hole of the middle partition directly be connected to constitute it is described instead
Ying Chi.
Further, the shape of the reaction tank is circle.
Further, the height of first feed pathway is less than the diameter of the microballoon.
Further, the microballoon is polystyrene microsphere.
It further, further include power plant module；Wherein,
The power plant module is used for as described in the microspheres solution, detection antibody, sample solution importing and waste liquid export
Chip body provides power.
Further, the top of the chip body be equipped be connected to first feed pathway sample feeding mouth, and
The microballoon injection port of the second feed pathway connection and the waste liquid outlet being connected to the liquid outlet channel.
Further, the reaction tank in the chip body rectangular array be distributed, it is each it is horizontally-arranged on each reaction
Second feed pathway in pond shares a microballoon injection port, and the first feed pathway of each reaction tank in each tandem shares one
The liquid outlet channel of sample feeding mouth, all reaction tanks shares a waste liquid outlet.
Further, the power plant module includes vacuum pump and pressure-regulating valve, the vacuum pump by pipeline with it is described
Waste liquid outlet connection, the pressure-regulating valve are arranged on the pipeline.
It further, further include fluorescence microscope, the objective table of the fluorescence microscope is arranged in the micro-fluidic chip
On.
To solve above-mentioned second technical problem, the present invention adopts the following technical scheme:
A kind of immunofluorescent detection method using above-mentioned Immunofluorescence test system, by power plant module by sample solution
And the microspheres solution of capture antibody modification passes through the first feed pathway respectively and the second feed pathway imports in reaction tank, capture is anti-
Microspheres solution that body has been modified and antigen cross incubation in reaction tank, are passed through detection by the second feed pathway after the completion of being incubated for
Whether antibody has fluorescence by observing microballoon under fluorescence microscope and determines whether sample solution has antigen, and then complete
The qualitative and quantitative analysis of sample solution.
Compared with prior art, the present invention has the advantage that
(1) height of liquid outlet channel is respectively less than the diameter of microballoon, so microballoon can only flow in the second feed pathway, and
And rest in reaction tank and reacted with subsequent sample and reaction reagent, entire immunoassay is completed, to eliminate using non-
The special constructions such as the micro- weir used when magnetic microsphere is as solid phase carrier or micro-valve, thus chip structure is simplified, reduce behaviour
Make cost.
(2) there is a plurality of independent test runner, may be implemented to launch the orientation of the microballoon for carrying different antibodies and
The parallel sample introduction of a variety of biological samples reaches the high speed to Multi-example while detecting, analysis time and cost is greatly saved.
(3) have many advantages, such as portability, ease for use as driving force using micro-fluidic chip and vacuum pump；Biological sample
It is simple with reagent adding method, equipment good seal, securely and reliably.
Detailed description of the invention
Fig. 1 is the main view of micro-fluidic chip of the present invention；
Fig. 2 is the explosive view of micro-fluidic chip of the present invention；
Fig. 3 is the perspective view of micro-fluidic chip of the present invention；
Fig. 4 is the structural schematic diagram of micro-fluidic chip system of the present invention；
Fig. 5 is that 4 kinds of samples are modified with the fluorogram obtained after the microballoon reaction of different capture antibody from 4 kinds respectively；
The relationship of human IgG sample and fluorescence intensity in Fig. 6 micro-fluidic chip.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1 and Fig. 3, a kind of Immunofluorescence test system based on micro-fluidic chip, including chip body 1, in
Portion is formed with one or more independently of one another for testing the test runner of sample to be tested；Test runner include reaction tank 2 and
The first feed pathway 3, the second feed pathway 4 and the liquid outlet channel 5 being connected to reaction tank 2；First feed pathway 3 is for being passed through sample
Product solution, the second feed pathway 4 are used to be passed through the microspheres solution and detection antibody of capture antibody modification, the height of liquid outlet channel 5
The respectively less than diameter of microballoon.To save analysis time and cost, test runner be preferably designed for it is a plurality of, so as to simultaneously to not
Same sample carries out immune detection.The top of chip body 1 be equipped be connected to the first feed pathway 3 sample feeding mouth 6, and
Second feed pathway 4 connection microballoon injection port 7 and be connected to liquid outlet channel 5 waste liquid outlet 8.
Specifically, microballoon uses diameter for 40 μm of polystyrene microsphere, the capture antibody of 500 μ g/ml is fixed on microballoon
On, it silanization treatment has been carried out to microsphere surface and has been closed with BSA using preceding to improve the fixed efficiency of antibody.
In the present embodiment, the microspheres solution and sample solution of antibody modification pass through sample feeding mouth 6 and microballoon sample introduction respectively
Mouth 7 imports in reaction tanks 2, and cross incubation in reaction tank 2, is passed through detection antibody from microballoon injection port 7 after the completion of being incubated for, glimmering
Determine whether sample solution has antigen by the way that whether observation microballoon has fluorescence under light microscope, so as to complete sample
The qualitative and quantitative analysis of solution.Because the height of liquid outlet channel 5 is respectively less than the diameter of microballoon, so microballoon can only be logical in the second feed liquor
It is flowed in road 4, and rests in reaction tank 2 and reacted with subsequent sample and reaction reagent, complete entire immunoassay, without
It is sucked out out of liquid outlet channel, so that it is special that the micro- weir used when making solid phase carrier using non-magnetic microspheres or micro-valve etc. is omitted
Structure.Therefore the manufacture craft for simplifying chip, reduces operating cost.
To prevent microballoon from entering in the first feed pathway, the height design of the first feed pathway is less than the diameter of microballoon.Into
One step, for the ease of the processing and fabricating of chip, the cross sectional shape and size of the first feed pathway 3 with the liquid outlet channel phase
Together.
Specifically, as shown in Fig. 2, the chip body 1 of micro-fluidic chip includes being sequentially overlapped and sealing to match in the present embodiment
Upper substrate 101, middle partition 102 and the lower substrate 103 of conjunction.Wherein, the end face indent that upper substrate 101 is in contact with middle partition 102
It is formed with the first feed pathway 3, first chamber 9 and the liquid outlet channel 5 being sequentially connected to；Lower substrate 103 is in contact with middle partition 102
End face be recessed the second liquid outlet channel 4 being sequentially connected to and second chamber 10；First chamber 9 and second chamber 10 pass through
The through-hole of partition is directly connected to and then constitutes reaction tank 2, first chamber 9, second chamber 10 and through-hole coaxial arrangement in perforation.This
In embodiment, the second feed pathway 4 is used to import the microballoon of adsorbable capture antibody.First feed pathway 3 is for importing sample
Solution, middle partition 102 are used for the cross-mixing of blocking solution, avoid pollution, and chip body 1 uses layer structure, so as to
Facilitate the processing of the fluid channel in chip body 1.
It is envisioned that upper substrate 101, middle partition 102 and lower substrate 103 can make poly- diformazan in actual design
Radical siloxane (PDMS), polycarbonate (PC), cyclic olefine copolymer (COC), silica gel, polytetrafluoroethylene (PTFE), poly-methyl methacrylate
The production of the high molecular materials such as ester (PMMA).
The specific manufacturing process of chip body is as follows: 103 material of the present embodiment upper substrate 101, middle partition 102 and lower substrate
It is dimethyl silicone polymer (PDMS), and is made by photoetching technique, and formpiston used in the casting of this micro-fluidic chip is then adopted
It is processed with wet process mask lithography, which has many advantages, such as that flexible high, low manufacture cost and period are short, uses Adobe
Illustrator CS5 software design microchannel structure, is then printed on a high-resolution transparent membrane, it is required that
The exposure mask of size, and manufactured using UV exposure and wet chemical etching technology.
Anode membrane manufacturing process is as follows: using ito thin film as the substrate of microchannel complex mold, it is suitable to cut size respectively
Du Pont's dry film and ito thin film, respectively remove Du Pont's dry film and ito thin film outer layer protective film, by dry film under the red light of darkroom
It is slowly equably attached on substrate, guarantees pasted dry film surfacing, bubble-free.The substrate for posting dry film is protected with blank sheet of paper,
It slow transits through plastic packaging machine 3 times, fits closely the two.Then, the printed exposure mask for having microchannel pattern is tightly attached to dry film
On, it is put into uv exposure box and exposes, the time for exposure sets according to institute's paste layer number.Mask pattern is transmitted under ultraviolet light
Light make dry film below be denaturalized, obtain corrosion-resisting pattern.The protective film for taking microchannel mask and Du Pont's dry film off, with 0.85%
Na2CO3Develop, to will only leave dry film figure against corrosion by the removing that is partly dissolved at the masked of microchannel in exposure process
After case, remaining Na is cleaned repeatedly with ultrapure water2CO3Aqueous solution simultaneously dries up, and heating removes remaining moisture in 60 DEG C of baking ovens,
Obtain microchip complex mold.
Upper substrate, middle partition and lower substrate recombination process are as follows: 5% PVA is uniformly coated on to the bottom made by PDMS
On plate and it is put into evaporation removing water formation PVA thin layer in 60 DEG C of baking oven, by silicon rubber prepolymer Sylgard 184 and curing agent
It is mixed well, is cast on the mold of microchannel with 10: 1 ratios, vacuumize degassing 30min, object to be mixed with vacuum pump using circulatory water
After middle bubble completely disappears, the PDMS bottom plate for being coated with PVA is covered into mold and places weight on PDMS bottom plate to obtain thickness
Spend the uniform middle layer met the requirements.It is in 2h in 60 DEG C of baking ovens and accelerates the pre- plastomer solidification of PDMS.After cooling, after solidification
PDMS thin layer peeled from template, PDMS middle layer is combined together with PVA at this time.It is beaten with punch in microchannel end
Inlet and outlet of the hole as solution, the one side with arc gum by PDMS substrate with pipeline carry out oxygen plasma with middle layer and live
Change, the combination of two panels chip is completed under microscope auxiliary positioning.Then, the chip after combination is put into distilled water and is dissolved
It PVA layers and dries, oxygen plasma activation, microchannel 25 is carried out to chip and another chip containing microchannel structure again
μm upper layer PDMS chip (upper substrate), lower layer's PDMS chip that 200 μm of middle layer (middle partition) and microchannel are 100 μm (under
Substrate) binding site of three layers of micro-structure PDMS is accurately positioned under micro- auxiliary positioning, it is then irreversible to be closely pasted onto one
Act the production for completing three-decker chip.
For convenience of observing under the microscope, the shape of reaction tank 3 is preferably designed for circle.
Referring to fig. 4, a kind of Immunofluorescence test system based on micro-fluidic chip, further includes power plant module；Wherein, power
Microballoon, detection antibody, sample solution and the export waste liquid that module is used to import for the micro-fluidic chip provide power.
Referring to figs. 2 and 3, it is contemplated that in practical applications, the rectangular array in chip body 1 of reaction tank 2
Distribution, it is each it is horizontally-arranged on the second feed pathway of each reaction tank 2 share a microballoon injection port, each reaction in each tandem
First feed pathway in pond 2 shares a sample feeding mouth, and the liquid outlet channel of all reaction tanks shares a waste liquid outlet.Such as this
In embodiment, it is in four rows, four column distribution, correspondingly, the number of sample feeding mouth and microballoon injection port that the quantity of reaction tank, which is 16,
Amount is four.
Referring to fig. 4, power plant module includes vacuum pump 11, pressure-regulating valve 12 and waste liquid reservoir 13, and vacuum pump 11 passes through
Pipeline 14 is connected to waste liquid reservoir 13, and waste liquid reservoir 13 is connect by emulsion tube with waste liquid outlet 8, and pressure-regulating valve 12 is set
Set on waste liquid reservoir 13, provide negative pressure by vacuum pump 11, can by microspheres solution and sample solution adsorption reaction pond,
Waste liquid after the completion of simultaneous reactions is sucked in waste liquid reservoir 13 by the negative pressure that vacuum pump 11 provides through emulsion tube, is passed through simultaneously
Adjust the size of the adjustable adsorptive pressure of pressure-regulating valve 12.
Specifically, the present embodiment micro-fluidic chip system further includes fluorescence microscope 15, the setting of micro-fluidic chip ontology 1 exists
On the objective table of fluorescence microscope 15, the specific structure as fluorescence microscope 15 is the prior art, is not improvement of the present invention
Point, details are not described herein.
Application Example
Microspheres solution (goat anti-chicken IgG, the goat of different capture antibody modifications are passed through to four microballoon injection ports 7 respectively
Anti-rabbit IgG, Goat anti-Human IgG, goat anti-mouse IgG), different sample solution (chicken IgG, rabbits are passed through to four sample feeding mouths 6
IgG, human IgG, mouse IgG), the microspheres solution and antigen for having modified capture antibody cross in reaction chamber to be incubated for and by pressure tune
The pressure for saving valve control vacuum pump, is passed through detection antibody from microballoon injection port 7 after the completion of being incubated for, passes through sight under fluorescence microscope
It examines whether microballoon has fluorescence to determine whether sample solution has antigen, as shown in Figure 5 and Figure 6, completes determining for sample solution
Property quantitative analysis.In Fig. 5 (a), (b), (c) and (d) respectively indicates the antigen chicken IgG that concentration is 0.1 μ g/mL, rabbit igg, people
IgG, the fluorescent image of mouse IgG, 1,2,3 and 4 respectively represents with capture antibodies Goat anti-chicken IgG, goat anti-rabbit igg, goat
The microballoon of anti-human igg and goat anti-mouse IgG modification.
The present invention ensures that microballoon is successfully introduced into chip reaction chamber using the vacuum degree of 0.02MPa, respectively on upper substrate top
4 microballoon injection ports injection in portion has modified 4 kinds of microballoons of different capture antibody and has injected 4 kinds of antigens with 4 sample feeding mouths
Solution uses the complete antigen 2min of buffer solution for cleaning unreacted after antigen-antibody reaction, inject after the completion of being incubated for corresponding
Antibody is detected, every kind of capture antibody only just has reaction to corresponding specific antigen, and three meets realization to target antigen
Real-time detection.The enzyme-linked immunosorbent assay chip system can carry out qualitative, quantitative rapidly and efficiently to a variety of biological samples simultaneously
Analysis, primary operation can determine the immunological response of more biological samples, and high-speed parallel analysis ability can provide efficiently just
Prompt instant detection, and the Development Techniques of micro-fluidic chip system also will be eventually for the portable micro-fluidic chip disease of exploitation
Diagnostic system minimizes diagnostic equipment further, portability, ease-of-use.
Above-described embodiment is only to clearly demonstrate examples made by the present invention, rather than the restriction to embodiment.For
For those of ordinary skill in the art, other various forms of variations or change can also be made on the basis of the above description
It is dynamic.Here without can not be also exhaustive to all embodiments.And the obvious variation or change thus amplified out
It is dynamic to be still in the protection scope of this invention.
Claims (10)
1. a kind of Immunofluorescence test system based on micro-fluidic chip, including chip body, inside be formed with one or more
Item is used to test the test runner of sample to be tested independently of one another, it is characterised in that:
The test runner includes reaction tank and the first feed pathway being connected to the reaction tank, the second feed pathway and goes out
Liquid channel；
First feed pathway is used to be passed through the micro- of capture antibody modification for being passed through sample solution, second feed pathway
Ball solution and detection antibody, the height of the liquid outlet channel are less than the diameter of the microballoon.
2. Immunofluorescence test system according to claim 1, it is characterised in that: the chip body includes being sequentially overlapped
And upper substrate, middle partition and the lower substrate of sealing cooperation；Wherein,
The end face that the upper substrate is in contact with the middle partition is recessed first feed pathway being sequentially connected to,
One chamber and the liquid outlet channel；
The end face that the lower substrate is in contact with the middle partition be recessed second liquid outlet channel being sequentially connected to and
Second chamber；
The first chamber is directly connected to by the through-hole of the perforation middle partition with second chamber and constitutes the reaction tank.
3. Immunofluorescence test system according to claim 1, it is characterised in that: the shape of the reaction tank is circle.
4. Immunofluorescence test system according to claim 1, it is characterised in that: the height of first feed pathway is small
In the diameter of the microballoon.
5. Immunofluorescence test system according to claim 1-4, it is characterised in that: further include power plant module；
Wherein,
The power plant module is used to import for the microspheres solution, detection antibody, sample solution and waste liquid exports the chip
Ontology provides power.
6. Immunofluorescence test system according to claim 5, it is characterised in that: the top of the chip body be equipped with
The sample feeding mouth of first feed pathway connection, the microballoon injection port being connected to second feed pathway and with it is described
The waste liquid outlet of liquid outlet channel connection.
7. Immunofluorescence test system according to claim 6, it is characterised in that: the reaction tank is in the chip body
The distribution of interior rectangular array, it is each it is horizontally-arranged on the second feed pathway of each reaction tank share a microballoon injection port, it is each vertical
First feed pathway of each reaction tank on row shares a sample feeding mouth, and the liquid outlet channel of all reaction tanks shares one and gives up
Liquid outlet.
8. Immunofluorescence test system according to claim 6 or 7, it is characterised in that: the power plant module includes vacuum
Pump and pressure-regulating valve, the vacuum pump are connect by pipeline with the waste liquid outlet, and the pressure-regulating valve is arranged described
On pipeline.
9. Immunofluorescence test system according to claim 5, it is characterised in that: it further include fluorescence microscope, the core
Piece ontology is arranged on the objective table of the fluorescence microscope.
10. a kind of detection method using Immunofluorescence test system as claimed in claim 9, it is characterised in that: pass through power
The microspheres solution of sample solution and capture antibody modification is passed through the first feed pathway to module respectively and the second feed pathway imports
In reaction tank, the microspheres solution of capture antibody modification and antigen cross incubations in reaction tank, pass through second after the completion of being incubated for
Feed pathway is passed through detection antibody, under fluorescence microscope by observe microballoon whether have fluorescence determine sample solution whether band
There is antigen, and then completes the qualitative and quantitative analysis of sample solution.
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