CN112798345A

CN112798345A - Pre-dilution mode sample collection and distribution system, method and blood cell analyzer

Info

Publication number: CN112798345A
Application number: CN202011592544.3A
Authority: CN
Inventors: 王兴红; 邹海涛
Original assignee: Shenzhen Comen Medical Instruments Co Ltd
Current assignee: Shenzhen Comen Medical Instruments Co Ltd
Priority date: 2020-12-29
Filing date: 2020-12-29
Publication date: 2021-05-14
Also published as: WO2022142052A1

Abstract

The application discloses sample collection and distribution system and method and hematology analyzer of predilution mode, including at least two detection ponds, first syringe that has first capacity, second syringe that has second capacity and the sampling subassembly that has the sampling needle, there are at least one count detection pond and at least one immunodetection pond in the detection pond, first syringe with the second syringe passes through first tube coupling, the second syringe with the sampling subassembly passes through the sampling tube coupling, each count detection pond through rather than the second tube coupling that corresponds with first syringe, each immunodetection pond through rather than the third tube coupling that corresponds with first syringe. The system only needs one sample collection process to absorb the mixed sample required by detection, increases the number of blood cells actually participating in the detection process, has small change of reagents and sample amount, has little change of a reaction system, and has stable detection result.

Description

Pre-dilution mode sample collection and distribution system, method and blood cell analyzer

Technical Field

The invention relates to the field of blood detection and analysis, in particular to a blood cell analyzer.

Background

Blood cell analyzers generally have two modes: whole blood mode and pre-dilution mode.

The whole blood mode is a general technology that a high-precision 100ul injector is connected with a sampling needle, the sampling needle is inserted into a blood collection tube after the blood collection tube is fully shaken up, and a power is provided by the 100ul injector to collect a blood sample into the sampling needle and a pipeline connected with the sampling needle. Current blood cell analyzers compare the complete patterns of CBC (measure the number of white blood cells, red blood cells and platelets), DIFF (four-class statistical technique for white blood cells) and CRP concentration measurement and or the reactive concentration measurement of SAA, so that a volume of 100ul is sufficient to provide a complete sample of CBC + DIFF + CRP counts.

However, in the actual detection process, some patients with difficulty in collecting venous blood, such as infants, fat people, severe patients and large-area burns, are inevitably encountered, and the pre-dilution mode is very suitable at the moment.

The sample in pre-dilution mode is prepared by mixing 20ul of peripheral blood sample and 180ul of diluent outside the machine to form a 1:10 mixed sample, collecting a certain amount of mixed sample by a sampling assembly of the instrument and then distributing the mixed sample into a detection cell inside the instrument. Therefore, if a 100ul syringe is used as the power source to collect 90ul of the mixed sample at the maximum in 90% capacity of the syringe, the volume of the blood cells contained in the mixed sample is preferably 9 ul. Since the sample is diluted by 10 times and the volume of real cells contained in the sucked mixed sample is small, the number of particles recognized by the blood cell analyzer and the number of pulses of received particle signals are far less than those in the whole blood mode, which may cause a risk of a large deviation between the detection result and the real value, and affect the judgment of a doctor.

The prior art has two main methods for solving the problems:

in the first method, a 100ul syringe is still used as a power device for collecting the pre-diluted sample, 80-90 ul samples are collected at one time, the rest actions are the same as the whole blood mode, and then the dilution ratio of the pre-dilution mode is changed through an algorithm to correct to obtain a new detection result. The method is simple to operate, changes little compared with a whole blood mode, but because the sample amount of blood cells actually participating in detection in the instrument detection process is too small, the sample adding action, the diluent adding action, the reaction reagent adding action and the diluting and mixing action involved in the counting process are all in error, meanwhile, the pre-dilution mode changes a reaction system of the sample and the reagent to a certain extent, the CRP and SAA detection adopts a latex immunoturbidimetry method to measure the reaction concentration, and different from the red blood cell and white blood cell detection which adopts a statistical counting mode, the results obtained by all methods of correcting the dilution ratio by the algorithm have some deviation and instability with actual values.

In the second method, taking the CBC + DIFF + CRP mode as an example, still using a 100ul syringe as a power source, 50ul of the diluted sample is first aspirated and added to the CRP reaction chamber to ensure that the actual amount of blood cells added to the CRP reaction chamber is 5ul, which is consistent with the whole blood mode, and then the sampling needle is returned to the sampling site to aspirate 80ul of the mixed sample for CBC and DIFF counting. The mode preferentially ensures accurate CRP counting, but the counting time is long due to multiple times of sample suction, so the detection speed is influenced, and meanwhile, the secondary sample suction mode is only suitable for some semi-automatic instruments, needs to fix a test tube seat at an open sampling position of the instrument, and is not suitable for some completely open models.

Disclosure of Invention

The present invention provides a new sample collection and distribution system.

The invention provides a sample collecting and distributing system in a pre-dilution mode, which comprises at least two detection pools, a first injector with a first capacity, a second injector with a second capacity and a sampling assembly with a sampling needle, wherein the detection pools are provided with at least one counting detection pool and at least one immunodetection pool; when the sample is dispensed, the stored part of the mixed sample is injected into the counting detection pool by the power provided by the first syringe, and the stored part of the mixed sample is injected into the immunoassay detection pool by the power provided by the second syringe; the initial position of the second syringe is in a set volume position, the set volume being less than the second volume, the second volume being less than the first volume.

The mixed sample is a mixed diluted sample formed by mixing a blood sample and a diluent according to a certain proportion.

The first capacity may be substantially greater than the second capacity, such as the first capacity being at least ten times greater than the second capacity. The set capacity may be half of the second capacity. When the sample is collected, the first injector is used as a power source, and all mixed samples required by detection are collected at one time.

The sample collection and distribution system further comprises a diluent container, wherein the first syringe or the second syringe is connected with the diluent container through a first diluent pipeline, and the first diluent pipeline is connected with a control component capable of controlling the on-off of the pipeline. When the first diluent pipeline is conducted, the first injector or the second injector can suck diluent from the diluent container.

Each detection pool is connected with the diluent container through a second diluent pipeline corresponding to the detection pool, and the second diluent pipeline is connected with a control component capable of controlling the on-off of the pipeline. Every detection pond all can be rather than the second diluent pipe connection that corresponds, through the drive of malleation source, can pour into the detection pond with the diluent in the diluent container.

A blood cell analyzer comprising the sample collection and dispensing system.

A method for collecting and dispensing a sample in a pre-dilution mode, wherein a first syringe having a first volume is connected to a second syringe having a second volume through a first tube, the first volume being larger than the second volume, the second syringe is connected to a sampling assembly having a sampling needle through a sampling tube, the first syringe is connected to a count detection cell through a second tube, and the first syringe is connected to an immunoassay detection cell through a third tube, the method further comprising the following steps performed in a set sequence:

a presetting step, namely enabling the initial position of the second injector to be at a position with a set volume, wherein the set volume is smaller than the second volume;

a sample collection step, wherein a mixed sample with a total volume required for detection is sucked by power provided by a first syringe, and the mixed sample is stored in the sampling needle and a sampling pipeline;

a first sample dispensing step of injecting a first volume of the mixed sample into the counting detection cell by power provided by a first syringe;

a second sample dispensing step of injecting a second volume of the mixed sample into the immunoassay reservoir by a power provided by a second syringe;

the first sample dispensing step and the second sample dispensing step are each performed at least once, and a sum of each of the first volumes and each of the second volumes is not greater than the total volume.

When the number of the counting detection pools is multiple, the first sample distribution step is executed for multiple times. When there are a plurality of immunoassay pools, the second sample dispensing step is performed a plurality of times.

The sample collecting and distributing method further comprises a mixing step of conducting the second pipeline or the third pipeline and mixing the liquid in the detection cell by repeatedly performing suction and discharge actions on the first injector.

The sample collection and distribution method comprises the steps that the first injector is connected with a diluent container through a first diluent pipeline, the method further comprises a cleaning step, the first diluent pipeline is conducted, the first pipeline is disconnected and conducted, and the first injector sucks diluent from the diluent container; and disconnecting and connecting the first diluent pipeline, connecting the first pipeline, and discharging the diluent through the sampling needle by the first injector.

The second injector is connected with a diluent container through a first diluent pipeline, and the method further comprises a first cleaning step, so that the second diluent pipeline is conducted, the sampling pipeline is disconnected and conducted, and the second injector sucks diluent from the diluent container; and the second diluent pipeline is disconnected and conducted, the sampling pipeline is conducted, and the second injector discharges the diluent through the sampling needle.

Each the detection pond through with its second diluent pipeline that corresponds with the diluent container is connected, still includes the second and washs the step, makes the second diluent pipeline switches on, and it is right through the drive of malleation source the diluent in the diluent container the detection pond washs.

The second syringe is connected to a diluent container through a first diluent line, and in the second sample dispensing step, the second syringe draws a diluent having a volume equal to the set volume from the diluent container. As the sampling assembly moves from one immunoassay reservoir to another, diluent in accordance with the set volume is replenished into the second syringe.

The invention has the beneficial effects that: only need the course of once gathering the sample to suck the mixed sample that the detection needs, increase the number of blood cells that actually participate in the testing process, reagent and sample amount change little, change little to the reaction system, the testing result is stable; and the mixed sample is injected into the immunodetection pool through the second injector with small capacity, so that the sample injection precision is improved.

Drawings

FIG. 1 is a schematic block diagram of a first embodiment of a sample collection and distribution system in pre-dilution mode;

FIG. 2 is a schematic diagram of the dispensing of a mixed sample of the first embodiment;

FIG. 3 is a schematic diagram of the structure of a second embodiment of the sample collection and distribution system in pre-dilution mode;

FIGS. 4 to 6 are schematic structural views of a second embodiment when liquids in three immunoassay wells are mixed uniformly;

FIG. 7 is a schematic diagram showing the change of the volume of liquid in each immunoassay cell with time when each immunoassay cell is sequentially washed in accordance with the second embodiment, wherein the abscissa is time, the ordinate is the volume of liquid in each immunoassay cell, the long bars indicate the washing time of the diluent, and the three black squares indicate the discharge time of the waste liquid port of each immunoassay cell, respectively.

Detailed Description

Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.

In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.

In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.

As shown in FIG. 1, a pre-dilution mode sample collection and dispensing system includes a first syringe 2, a second syringe 5, a sampling assembly 6, and at least two test cells. The first syringe 2 has a first capacity capable of serving as a power source for sucking and discharging a liquid. The second syringe 5 has a second capacity capable of serving as a power source for sucking and discharging the liquid, and the second capacity is smaller than the first capacity. The sampling assembly 6 has a sampling needle which is capable of aspirating a sample and discharging a sample. Among the detection pools, there are at least one immunoassay detection pool 1 and at least one counting detection pool. The initial position of the second syringe corresponds to a set volume that is less than the second volume. The first capacity may be substantially greater than the second capacity, such as the first capacity being at least ten times greater than the second capacity. The set capacity may be half of the second capacity. Specifically, the first volume may be 2.5ml, the second volume may be 100ul, and the set volume may be 50 ul.

The first syringe 2 is connected to the second syringe 5 by a first tubing, and the second syringe 5 is connected to the sampling needle of the sampling assembly 6 by a sampling tubing 105. Each counting detection pool is connected with the first injector 2 through a second pipeline corresponding to the counting detection pool, and each immunodetection pool 1 is connected with the first injector 2 through a third pipeline corresponding to the immunodetection pool.

The system can realize sample collection and sample distribution according to set time sequence. When the sample is collected, the first pipeline and the sampling pipeline 105 are both communicated, the first injector 2 is used as a power source, the mixed sample is sucked by the sampling needle, and the mixed sample is stored in the sampling needle and the sampling pipeline 105. When a sample is dispensed to the immunoassay cell 1, the sampling line 105 is opened, and the stored partially mixed sample is injected into the immunoassay cell 1 through the sampling needle using the second syringe 5 as a power source. When the sample is dispensed to the counting chamber, both the first line and the sampling line 105 are conducted, and the stored part of the mixed sample is injected into the counting chamber using the first syringe 2 as a power source.

When the sample is collected, the total volume of the collected mixed sample is larger than the sum of the volumes of the mixed samples distributed to the immunoassay cells and the counting detection cells, namely, the sample collection is only needed once.

The system can also comprise a diluent container 120, wherein the diluent container 120 is connected with the high-capacity first injector 2 through a diluent pipeline, a control component capable of controlling the on-off of the pipeline is arranged on the diluent pipeline, the control component has an open state and a closed state, and the diluent pipeline is conducted in the open state; in the closed state, the diluent pipeline is disconnected and communicated. When the control means is in the open state, the diluent container and the first syringe 2 are communicated, so that the first syringe 2 can suck the diluent from the diluent container 120.

In another structure, the diluent container 120 and the small-capacity second syringe 5 may be connected by a diluent pipeline, the diluent pipeline is provided with a control component capable of controlling the on-off of the pipeline, the control component has an open state and a closed state, and in the open state, the diluent pipeline is conducted; in the closed state, the diluent pipeline is disconnected and communicated. When the control means is in the open state, the diluent container and the second syringe 5 are communicated, so that the second syringe 5 can suck the diluent from the diluent container 120.

This system can also carry out the mixing to the liquid in the detection pond, during the mixing to first syringe 2 is as the power supply, carries out the operation of inhaling repeatedly and telling to the liquid in the detection pond, realizes the mixing to liquid.

The system can also clean the detection pool, and when the system is cleaned, the first injector 2 is used as a power source to clean the pipeline and the detection pool by driving diluent under pressure.

Referring to fig. 1 and 2, a pre-dilution mode sample collection and distribution system for a blood cell analyzer is mainly applied to a blood routine plus an immunoassay project mode. The system comprises an immunoassay pool 1, a first injector 2, a two-position three-way electromagnetic valve 3, a two-position three-way electromagnetic valve 4, a second injector 5, a sampling assembly 6 and

pipelines

101, 102, 103, 104, 105 and 106. The first syringe 2 has a first capacity, e.g. 2.5ml, and the second syringe 5 has a second capacity, e.g. 100 ul. The initial position of the second syringe 5 is in a set volume position, e.g. 50ul, i.e. the initial position of the second syringe piston corresponds to a volume of 50 ul.

The first injector 1 is connected with a first port of the two-position three-way electromagnetic valve 3 through a pipeline 102, a second port of the two-position three-way electromagnetic valve 3 is connected with a first port of the two-position three-way electromagnetic valve 4 through a pipeline 103, a third port of the two-position three-way electromagnetic valve 4 is connected with a port of the second injector 5 through a pipeline 104, and a second port of the two-position three-way electromagnetic valve 4 is connected with a diluent container 120 through a pipeline 106. The third port of the two-position three-way electromagnetic valve 3 is connected with an immunodetection pool 1 through a pipeline 101, and the immunodetection pool 1 is provided with a waste liquid port 7. The other port of the second syringe 5 is connected with a sampling assembly 6 through a sampling pipeline 105, and the sampling assembly 6 can be connected with a waste liquid pump 121. For the two-position three-way electromagnetic valves 3 and 4, when power is lost, the first port is communicated with the second port; when power is supplied, the first port and the third port are communicated.

The working process of the system is as follows:

step 1: the pre-dilution mode is performed as follows:

when entering a pre-dilution mode, calling a time sequence from a whole blood mode to the pre-dilution mode, converting the preparation state of the instrument into a state of the pre-dilution mode, and setting the position of a second syringe 5 to a position with the capacity of 50ul in the time sequence;

executing a counting sequence of the corresponding pre-dilution mode, and still setting the initial position of the second injector 5 as the position with the capacity of 50ul in the ending process of the sequence;

judging whether the next sample exits from the pre-dilution mode when the execution of the counting time sequence is finished, and if not, continuing to execute according to the step two; if yes, executing the step (iv);

quitting the pre-dilution mode, executing the pre-dilution to whole blood mode, converting the preparation state of the instrument from the pre-dilution mode state to the whole blood mode state, and restoring the second injector 5 to the initial position of the whole blood mode.

By now, with the above procedure, it is ensured that the second syringe 5 is in a position with a capacity of 50ul at the beginning of each measurement in pre-dilution mode, and that it does not affect the whole blood mode.

Step 2: after the instrument enters a pre-dilution mode, the sampling assembly 6 moves to a sampling position, the two-position three-way electromagnetic valve 4 is electrified, then the first injector 2 is communicated with the

pipelines

102, 103, 104 and 105, 160ul of mixed sample is sucked by taking the first injector 2 as power, and the sucked mixed sample is stored in a sampling needle and a sampling pipeline 105 of the sampling assembly 6.

And step 3: the sampling component 6 moves to the position of the immunoassay cell, the second injector 5 is used as power, 50ul of stroke is pushed outwards, the sample adding and distributing process to the immunoassay cell 1 is completed, and the actual reaction blood sample of the immunoassay cell 1 is 5ul under the pre-dilution and whole blood modes, so that the reaction system is almost unchanged under the two modes; meanwhile, the second injector 5 with the capacity of 100ul is used as the power for adding the immune detection sample, so that the sample adding error can be controlled in a very small range.

And 4, step 4: after the sample distribution of the immunoassay detection cell is completed, the sampling assembly 6 moves into a WBC detection cell (not shown in the figure), and as shown in fig. 1, the two-position three-way solenoid valve 4 is powered to communicate the first injector 2 with the

pipelines

102, 103, 104, 105 and the sampling needle, and then the first injector 2 is used as a power source to distribute 64ul of samples required by CBC counting to the WBC detection cell, so as to ensure that the sample distribution of CBC counting in the reaction blood sample mode and the whole blood mode is 6.4 ul;

and 5: after the step 4 is completed, the sampling assembly 6 is moved into a DIFF detection cell (not shown in the figure), as shown in fig. 1, the two-position three-way solenoid valve 4 is powered on, so that the first injector 2 is communicated with the

pipelines

102, 103, 104, 105 and the sampling needle, then the first injector 2 is used as a power source, DIFF (four-class statistics of white blood cells) is distributed to the DIFF detection cell to count 40ul of required samples, 8ul of blood samples are theoretically required in a whole blood mode, and therefore the dilution ratio needs to be corrected in a pre-dilution mode. Since the DIFF detection pool is used for detecting the percentage of four categories of the white blood cells, the requirement on the number of particles is not high, and therefore the influence on the correction dilution comparison result is not large.

To this end, the pre-dilution mode of sample collection and dispensing has been completed, with blood sample dispensing as shown in FIG. 2, with the trailing volume V1 of sample being purged along with the cleaning of the sampling needle and tubing.

As shown in figure 1, first syringe 2 can be used for mixing the liquid in the immunoassay pond 1 evenly simultaneously, and after sample and reagent all added in immunoassay pond 1, two tee bend solenoid valves 3 commutate after getting electric, and first syringe 2 passes through

pipeline

102, 101 and links to each other with immunoassay pond 1, absorbs and discharges the action through first syringe 2 self repetition, realizes the rolling mixing of the inside liquid of immunoassay pond 1.

As shown in fig. 1, the first injector 2 can be used for cleaning the immunoassay pool 1 and the pipeline 101 connected with the immunoassay pool, in a normal state, the first injector 2 is pulled down to absorb a certain amount of diluent, then the two-position three-way electromagnetic valve 3 is powered on, the pipeline 102 is communicated with the pipeline 101, the first injector 2 discharges the diluent which is just absorbed, the discharged diluent washes the

pipelines

102 and 101 along the way, finally reaches the immunoassay pool 1, washes the immunoassay pool 1, and the washed waste liquid is discharged from the waste liquid port 7 at the bottom of the immunoassay pool 1, and the washing process is completed by repeating the steps.

As shown in fig. 1, the first syringe 2 can be used to clean the sampling needle and the sample tube 105 of the sampling assembly 6, after the first syringe 2 sucks the diluent from the diluent container 120, the two-position three-way solenoid valve 4 is electrically switched to communicate the first syringe 2 with the tube 104, the second syringe 5, the tube 105 and the sampling needle of the sampling assembly 6, then the first syringe 2 discharges the sucked diluent outwards, the discharged diluent cleans the tube and the inner wall of the sampling needle along the way, and the cleaned diluent is collected and discharged by the negative pressure provided by the waste liquid pump 121 connected to the sampling assembly 6.

In this embodiment, the first syringe 2 and the second syringe 5 are connected by a first pipeline, the first syringe 2 and the diluent container 120 are connected by a diluent pipeline, the first syringe 2 and the immunoassay cell 1 are connected by a third pipeline, the first pipeline, the third pipeline and the diluent pipeline can be composed of multiple sections of pipelines, and the first pipeline, the third pipeline and the diluent pipeline can have a common pipeline. The

lines

102, 103 may be a common line of the first line and the diluent line. Line 102 may be a common line of the first and third lines. The first pipeline can be connected with the two-position two-way electromagnetic valves 3 and 4, and when the two-position two-way electromagnetic valves 3 and 4 are both de-energized, the diluent pipeline is conducted; when the two-position two-way electromagnetic valve 3 is electrified, the third pipeline is conducted; when the two-position two-way electromagnetic valve 3 is powered off and the two-position two-way electromagnetic valve 4 is powered on, the first pipeline is conducted.

For the system, the devices and pipelines such as the syringes, the electromagnetic valves and the like are repeatedly used for many times, so that the system is simplified, and the uniform mixing and cleaning of the immunodetection pool, the sample collection and the sample cleaning are realized.

As shown in fig. 3-7, a second embodiment of a pre-dilution mode sample collection and dispensing system, as applied to blood routine plus three immunoassay items.

The sample collection and dispensing system includes a first syringe 4, a second syringe 5, a sampling assembly 6 having a sampling needle, a diluent container 120, and multiple sections of tubing. The first injector 4 is connected with one interface of the second injector 5 through

pipelines

112 and 119, the other interface of the second injector 5 is connected with a first port of a two-position three-way electromagnetic valve V4 through a pipeline 113, a third port of the two-position three-way electromagnetic valve V4 is connected with the sampling assembly 6 through a pipeline 115, and the sampling assembly 6 is connected with a waste liquid pump 121. The second port of the two-position three-way solenoid valve V4 is connected to the second port of the two-position three-way solenoid valve V6 via a line 114, and the first port of the two-position three-way solenoid valve V6 is connected to the diluent container 120 via a line 116. The third port of the two-position, three-way solenoid valve V6 is connected to the second port of the two-position, three-way solenoid valve V5 by line 110. The third port of the two-position three-way solenoid valve V5 is connected to the line 111, and the

lines

111, 119, and 112 are communicated through a three-way joint J4. A first port of the two-position three-way solenoid valve V5 is connected to the common line 108. The line 107 and the line 106 are connected by a two-position two-way solenoid valve V3 to form a first branch line. The line 103 and the line 104 are connected by a two-position two-way solenoid valve V2 to form a second branch line. The line 102 and the line 101 are connected by a two-position two-way solenoid valve V1 to form a third branch line. The common pipeline 108 is connected with one end of the first, second and third branch pipelines through three-way joints J3, J2 and J1, and the other end of the first, second and third branch pipelines is connected with the immunodetection pools 1, 2 and 3. The

immunoassay cells

1, 2, 3 have

waste ports

7, 8, 9, respectively. For the two-position two-way solenoid valves V4, V5 and V6, when power is lost, the first port is communicated with the second port; when power is supplied, the first port and the third port are communicated. The two-position two-way solenoid valves V1, V2, and V3 are normally closed solenoid valves.

Step 1: the initial position of the second syringe 5 at the start of the pre-dilution mode is at the stroke 50ul, and the method of setting and execution is in accordance with the first embodiment.

Step 2: after the instrument enters a pre-dilution mode, the sampling assembly 6 moves to a sampling position, the two-position three-way electromagnetic valve V4 is electrified, the pipeline 113 is communicated with the pipeline 115, the first injector 4 is connected with the sampling assembly 6 through the pipeline 119 and the pipeline 113, the pipeline 115 is connected with the sampling assembly 4, 260ul of mixed sample is sucked through the power provided by the first injector 4, and the sucked mixed sample is stored in the sampling needle and the sampling pipeline 115 of the sampling assembly 6.

In the embodiment, three immune channels are adopted for adding blood for routine detection items, so that the mixed sample can be mixed by 30ul of blood sample and 270ul of diluent to form a mixed diluted sample with the ratio of 1: 10.

And step 3: after the step 2 is completed, the sampling assembly 6 moves to the immunoassay pool 1, then the two-position three-way electromagnetic valve V4 is electrified, so that the pipeline 113 is communicated with the pipeline 115, the second injector 5 is used as a power source, 50ul of mixed sample is pushed outwards, and the process of filling 50ul of mixed sample into the immunoassay pool 1 is completed.

And 4, step 4: after the distribution of the sample in the immunoassay pool 1 is completed, the sampling assembly 6 moves from the immunoassay pool 1 to the immunoassay pool 2, meanwhile, the second injector 5 sucks 50ul of stroke inwards, and the second injector 5 is connected with the diluent container 120 through the pipeline 113, the pipeline 114 and the pipeline 116, so that the 50ul of stroke sucked by the second injector 5 is completely filled with the diluent, an isolated gas column section cannot be generated, the internal pressure balance cannot be influenced, and the accuracy of subsequent distribution samples cannot be influenced.

And 5: after the step 4 is completed, the two-position three-way electromagnetic valve V4 is electrified, so that the pipeline 113 is communicated with the pipeline 115, the second injector 5 is used as power, 50ul of stroke is pushed outwards, and the process of filling 50ul of mixed sample into the immunoassay pool 2 is completed.

And 6, after the step 5 is completed, moving the sampling component 6 from the immunoassay pool 2 to the immunoassay pool 3, and sucking 50ul of stroke inwards by the second syringe 5 while moving the sampling component 6, wherein the stroke is completely filled with the diluent.

And 7: after the step 6 is completed, the two-position three-way electromagnetic valve V4 is electrified, so that the pipeline 113 is communicated with the pipeline 115, the second injector 5 is used as a power source, 50ul of stroke is outwards pushed, and the process of filling 50ul of mixed sample into the immunoassay pool 3 is completed.

So far, the sample distributing action of the three immune channels is finished, and according to the action, the sample distributing action can be ensured to be finished by using the second injector 5 with high precision and small capacity each time.

And 8: after the sample distribution of the immunoassay detection cell is completed, the sampling assembly 6 moves into the WBC cell (not shown in the figure), the two-position three-way solenoid valve V4 is powered, so that the first syringe 4 is communicated with the pipeline 119, the pipeline 113, the pipeline 115 and the sampling needle of the sampling assembly 6, and then the first syringe 4 is used as a power source to distribute 64ul of samples required by CBC counting to the WBC cell, thereby ensuring that the sample distribution of CBC counting is 6.4ul in a reaction blood sample and whole blood mode.

And step 9: after the step 8 is completed, the sampling assembly 6 is moved into a DIFF cell (not shown in the figure), the two-position three-way solenoid valve V4 is powered on, so that the first injector 4 is communicated with the pipeline 119, the pipeline 113, the pipeline 115 and the sampling needle of the sampling assembly 6, then the first injector 4 is used as a power source, DIFF (four-classification statistical technique of white blood cells) is distributed to the DIFF cell to count 40ul of required samples, and the dilution ratio in the corresponding pre-dilution mode is modified.

To this end, the pre-dilution mode of sample collection and distribution for the three immune channels has been completed and the remaining unused tail of the blood sample in the tubing is purged along with the cleaning of the sampling needle and tubing.

As shown in fig. 3 and 4, the first injector 4 can mix the immunodetection pools 1, 2 and 3. After the sample and the reagent are added into the immunoassay cell 1, the two-position three-way solenoid valve V5 is powered on, the two-position three-way solenoid valve V6 is powered on, so that the other liquid path of the first injector 4 is blocked by the two-position three-way solenoid valve V6 (namely, the liquid path where the pipeline 119, the pipeline 113 and the pipeline 114 are located is disconnected and communicated with the pipeline 116 because the two-position three-way solenoid valve V6 is powered on), then the two-position two-way solenoid valve V3 is powered on, the first injector 4 is communicated with the immunoassay cell 1 through the pipeline 112, the pipeline 111, the pipeline 108, the pipeline 107 and the pipeline 106, as shown in fig. 4, power is provided through the back-and-forth sucking and spitting actions of the first injector 4, and the liquid in the immunoassay. The mixing of the immunoassay pool 2 is as shown in fig. 5, when mixing, the two-position three-way electromagnetic valve V6, the two-position three-way electromagnetic valve V5 and the two-position two-way electromagnetic valve V2 are powered, and the first injector 4 is communicated with the immunoassay pool 2 through the pipeline 112, the pipeline 111, the pipeline 108, the pipeline 103 and the pipeline 104. The mixing of the immunoassay pool 3 is as shown in fig. 6, when mixing, the two-position three-way electromagnetic valve V6, the two-position three-way electromagnetic valve V5 and the two-position two-way electromagnetic valve V1 are powered, and the first injector 4 is communicated with the immunoassay pool 3 through the pipeline 112, the pipeline 111, the pipeline 108, the pipeline 102 and the pipeline 101.

In the process of mixing, the mixture of the sample and the reagent in the immunoassay cell will be sucked into the pipeline 101, the pipeline 104 or the pipeline 106, and even exceed the two-position two-way electromagnetic valve V1, the two-position two-way electromagnetic valve V2 or the two-position two-way electromagnetic valve V3, so generally, the

pipelines

101, 104 and 106 are roughly selected from teflon pipes with the pipe diameter of 1.0mm and the length of which is greater than 750mm, so that at least 500ul of volume can be borne, and the reaction liquid of the liquid in the three

immunoassay cells

1, 2 and 3 can not pollute the adjacent detection channels in the action of sucking, spitting and mixing. Simultaneously, two lead to solenoid valves V1, V2, V3 can use the double-layered tubular solenoid valve for the reaction liquid of mixing need not contact with solenoid valve V1, V2, V3 inner wall, but with the tight hose contact of valve clamp, avoid appearing the reaction liquid because inside the washing of solenoid valve is unclean, bring the residue, and then pollute next sample.

As shown in FIG. 3, the system can realize rapid washing of three immunoassay wells. The diluent container 120 is connected to the diluent tank 122 outside the machine, and when the two-position two-way solenoid valve V8 is energized, the diluent container 120 is connected to the negative pressure source 124, and the diluent is sucked from the diluent tank 122 by the suction force of the negative pressure source 124 and stored inside the diluent container 120. The negative pressure source 124 may be an external source of air or negative pressure created by pneumatic components inside the instrument.

As shown in fig. 3, the system can flush the immunoassay cell, taking flushing of the immunoassay cell 1 as an example for illustration, when the detection process is performed to finally flush the immunoassay cell 1, firstly, the reaction liquid in the original detection cell is drained through the waste liquid port 7 at the bottom of the immunoassay cell 1, then the two-position three-way solenoid valve V6 is powered, the two-position two-way solenoid valve V3 is powered, so that the

pipelines

106, 107, 108, 110 and 116 are connected, then the two-position two-way solenoid valve V7 is powered, the continuous flushing pipeline of the liquid in the diluent container 120 and the immunoassay cell 1 are pushed through the positive pressure source 123, simultaneously, the waste liquid port 7 is opened periodically, the flushed diluent is timely drained, and liquid overflowing is avoided, and the liquid condition in the immunoassay cell in the whole flushing process is shown in fig. 7. Compared with the existing cleaning mode that the syringe sucks a section of cleaning liquid and then discharges the cleaning liquid, the cleaning mode of the embodiment uses the positive pressure source 123 as a power source, does not need time consumed in the liquid sucking process, and is cleaned in the whole process, so that higher cleaning force can be provided, and the cleaning speed is extremely high, so that the effect of the cleaning mode is more obvious under the condition that the number of detection channels is more.

As shown in fig. 3, the system may also perform the function of purging the second syringe and sampling line as described in the first embodiment. The first injector 4 sucks a certain amount of diluent from the diluent container 120, then the two-position three-way electromagnetic valve V4 is electrically switched, so that the first injector 4 is communicated with the pipeline 119, the second injector 5, the pipeline 113, the pipeline 115 and the sampling needle of the sampling assembly 6, then the diluent sucked outwards by the first injector 4 is discharged, the pipeline along the way and the inner wall of the sampling needle are cleaned, and the cleaned diluent is collected and discharged through negative pressure provided by a waste liquid pump 121 connected with the sampling assembly.

The foregoing is a more detailed description of the present invention that is presented in conjunction with specific embodiments, and the practice of the invention is not to be considered limited to those descriptions. It will be apparent to those skilled in the art that a number of simple derivations or substitutions can be made without departing from the inventive concept.

Claims

1. A sample collection and dispensing system in pre-dilution mode comprising at least two test cells, a first syringe having a first capacity, a second syringe having a second capacity, and a sampling assembly having a sampling needle, the detection pool is provided with at least one counting detection pool and at least one immunity detection pool, the first injector and the second injector are connected through a first pipeline, the second injector is connected with the sampling assembly through a sampling pipeline, each counting detection pool is connected with the first injector through a second pipeline corresponding to the counting detection pool, each immunodetection pool is connected with the first injector through a third pipeline corresponding to the immunodetection pool, when a sample is collected, drawing a mixed sample by the power provided by the first syringe, storing the mixed sample in the sampling needle and sampling line; when the sample is dispensed, the stored part of the mixed sample is injected into the counting detection pool by the power provided by the first syringe, and the stored part of the mixed sample is injected into the immunoassay detection pool by the power provided by the second syringe; the initial position of the second syringe is in a set volume position, the set volume being less than the second volume, the second volume being less than the first volume.

2. The sample collection and dispensing system according to claim 1, further comprising a diluent container, wherein the first syringe or the second syringe is connected to the diluent container by a first diluent line, and wherein the first diluent line is connected to a control component capable of on-off control of the line.

3. The specimen collection and dispensing system of claim 2, wherein each of the test cells is connected to the diluent container by a second diluent line corresponding thereto, the second diluent line being connected to a control component capable of on-off control of the line.

4. A blood cell analyzer comprising the sample collection and distribution system of any one of claims 1-3.

5. A sample collecting and distributing method in a pre-dilution mode is characterized in that a first syringe with a first volume is connected with a second syringe with a second volume through a first pipeline, the first volume is larger than the second volume, the second syringe is connected with a sampling assembly with a sampling needle through a sampling pipeline, the first syringe is connected with a counting detection pool through a second pipeline, and the first syringe is connected with an immunity detection pool through a third pipeline, and the method further comprises the following steps of performing the following steps according to set time sequence:

6. The sample collection and distribution method according to claim 5, further comprising a mixing step of conducting the second line or the third line and mixing the liquid in the detection cell by repeating the suction operation and the discharge operation of the first syringe.

7. The sample collection and dispensing method of claim 5, wherein the first syringe is connected to a diluent reservoir via a first diluent line, further comprising a purging step to open the first diluent line and to disconnect the first line, the first syringe drawing diluent from the diluent reservoir; and disconnecting and connecting the first diluent pipeline, connecting the first pipeline, and discharging the diluent through the sampling needle by the first injector.

8. The sample collection and dispensing method of claim 5, wherein the second syringe is connected to a diluent container via a first diluent line, further comprising a first purging step to turn on the second diluent line and turn off the sampling line, the second syringe drawing diluent from the diluent container; and the second diluent pipeline is disconnected and conducted, the sampling pipeline is conducted, and the second injector discharges the diluent through the sampling needle.

9. The method of claim 8, wherein each of the test wells is connected to the diluent container via a corresponding second diluent line, and further comprising a second washing step of turning on the second diluent line and washing the test wells with the diluent in the diluent container driven by a positive pressure source.

10. The sample collection and dispensing method of claim 5, wherein the second syringe is connected to a diluent container via a first diluent line, and wherein the second syringe draws diluent from the diluent container in accordance with the set volume during the second sample dispensing step.