CN116859071B - Full-automatic chemiluminescence immunoassay analyzer and detection analysis method thereof - Google Patents
Full-automatic chemiluminescence immunoassay analyzer and detection analysis method thereof Download PDFInfo
- Publication number
- CN116859071B CN116859071B CN202311136417.6A CN202311136417A CN116859071B CN 116859071 B CN116859071 B CN 116859071B CN 202311136417 A CN202311136417 A CN 202311136417A CN 116859071 B CN116859071 B CN 116859071B
- Authority
- CN
- China
- Prior art keywords
- liquid
- needle
- cleaning
- sample
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000003018 immunoassay Methods 0.000 title claims abstract description 19
- 238000001514 detection method Methods 0.000 title claims abstract description 17
- 238000004458 analytical method Methods 0.000 title claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 414
- 238000004140 cleaning Methods 0.000 claims abstract description 230
- 239000002699 waste material Substances 0.000 claims abstract description 156
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 153
- 230000005284 excitation Effects 0.000 claims abstract description 145
- 238000005406 washing Methods 0.000 claims description 85
- 238000002347 injection Methods 0.000 claims description 13
- 239000007924 injection Substances 0.000 claims description 13
- 238000005259 measurement Methods 0.000 claims description 8
- 230000002572 peristaltic effect Effects 0.000 claims description 8
- 238000005086 pumping Methods 0.000 claims description 6
- 238000007599 discharging Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 5
- 238000005070 sampling Methods 0.000 abstract description 3
- 238000012864 cross contamination Methods 0.000 abstract 1
- 238000002425 crystallisation Methods 0.000 abstract 1
- 230000008025 crystallization Effects 0.000 abstract 1
- 238000007885 magnetic separation Methods 0.000 abstract 1
- 230000037452 priming Effects 0.000 description 66
- 239000000243 solution Substances 0.000 description 24
- 238000010926 purge Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 9
- 239000000758 substrate Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a full-automatic chemiluminescence immunoassay analyzer and a detection analysis method thereof. The analyzer comprises a liquid container module, a cleaning waste liquid module, a reagent sample filling module, a cleaning filling module and an excitation liquid filling module. The full-automatic chemiluminescence immunoassay analyzer provided by the invention has the effects of improving the accuracy of sampling and liquid adding, avoiding pipeline crystallization blockage, achieving accurate magnetic separation and cleaning, preventing cross contamination and the like during detection, and can reduce human errors and improve the consistency and repeatability of detection through automatic operation.
Description
Technical Field
The invention relates to the field of chemical detection equipment, in particular to a full-automatic chemiluminescence immunoassay analyzer and a detection analysis method thereof.
Background
Chemiluminescent immunoassay is a relatively advanced immunoassay technology accepted by the current medical community, has high accuracy and specificity, and is one of the most important technologies in the inspection method.
The full-automatic luminous equipment has important significance and wide prospect in the medical field, can be applied to aspects of clinical diagnosis, prevention control, personalized treatment and the like, and provides a quick and reliable detection result for medical staff.
In the full-automatic luminous equipment complete machine, the liquid path system is particularly important for the accuracy and consistency of detection, and in the detection, the accuracy, speed and efficiency of steps such as sampling, liquid adding and cleaning are the core of the whole machine, the manual operation is time-consuming and labor-consuming, the error of the detection result is larger, and the consistency and repeatability of the detection are lower.
Disclosure of Invention
In order to solve the problems, the invention provides a full-automatic chemiluminescence immunoassay analyzer and a detection analysis method thereof.
According to one aspect of the present invention, there is provided a full-automatic chemiluminescence immunoassay analyzer comprising a liquid container module, a wash waste liquid module, a reagent sample filling module, a wash filling module, and an excitation liquid filling module;
the liquid container module comprises a waste liquid container, a strong washing liquid container and a washing liquid container, wherein the waste liquid container is connected with a waste liquid pipeline, the strong washing liquid container is connected with a strong washing liquid pipeline, and the washing liquid container is connected with a washing liquid pipeline;
the cleaning waste liquid module comprises four waste liquid sucking needles, wherein each waste liquid sucking needle is connected with the waste liquid pipeline, and a waste liquid sucking peristaltic pump is arranged on the connecting pipeline;
the reagent sample filling module comprises a sample needle, a reagent needle, a sample cleaning pool, a reagent cleaning pool, a sample plunger pump and a reagent plunger pump, wherein one end of the sample needle is positioned right above the sample cleaning pool, the other end of the sample needle is connected with the sample plunger pump, one end of the reagent needle is positioned right above the reagent cleaning pool, the other end of the reagent needle is connected with the reagent plunger pump, the sample plunger pump and the reagent plunger pump are both connected with the cleaning liquid pipeline and are provided with a reagent sample inner wall cleaning pump on the connecting pipeline, the outer wall of the sample cleaning pool and the outer wall of the reagent cleaning pool are both connected with the cleaning liquid pipeline and are provided with a reagent sample outer wall cleaning pump on the connecting pipeline, and the bottom of the sample cleaning pool and the bottom of the reagent cleaning pool are both connected with the waste liquid pipeline and are provided with a waste liquid pump on the connecting pipeline;
the cleaning and filling module comprises three cleaning and filling needles, three cleaning needle filling liquid receivers, three cleaning needle three-way valves and three cleaning needle plunger pumps, wherein one end of each cleaning and filling needle is positioned right above each cleaning needle filling liquid receiver, the other end of each cleaning and filling needle is connected with the first end of each cleaning needle three-way valve, the second end of each cleaning needle three-way valve is connected with each cleaning needle plunger pump, the third end of each cleaning needle three-way valve is connected with a cleaning liquid pipeline, and the bottom end of each cleaning needle filling liquid receiver is connected with the waste liquid pipeline and is provided with a waste liquid collecting pump on the connecting pipeline;
the excitation liquid filling module comprises a pre-excitation liquid filling needle, an excitation liquid filling needle, a pre-excitation liquid filling connector, an excitation liquid filling connector, a pre-excitation liquid three-way valve, an excitation liquid three-way valve, a pre-excitation liquid plunger pump, an excitation liquid plunger pump, two pre-excitation liquid containers and two excitation liquid containers, wherein one end of the pre-excitation liquid filling needle is positioned right above the pre-excitation liquid filling connector, the other end of the pre-excitation liquid filling needle is connected with the first end of the pre-excitation liquid three-way valve, the second end of the pre-excitation liquid three-way valve is connected with the pre-excitation liquid plunger pump, the third end of the pre-excitation liquid three-way valve is respectively connected with the two pre-excitation liquid containers, one end of the excitation liquid filling needle is positioned right above the excitation liquid filling connector, the other end of the excitation liquid three-way valve is connected with the first end of the excitation liquid three-way valve, the second end of the excitation liquid valve is connected with the excitation liquid plunger pump, and the third end of the excitation liquid filling needle is respectively connected with the two pre-excitation liquid three-way valve, and the excitation liquid three-way valve is respectively connected with the excitation liquid container and the liquid filling connector.
In some embodiments, a sample needle inner wall cleaning valve and a reagent needle inner wall cleaning valve are respectively arranged on the pipeline connecting the sample plunger pump and the reagent plunger pump with the reagent sample inner wall cleaning pump. The device has the advantages that the sample plunger pump and the pipeline connecting the reagent plunger pump and the reagent sample inner wall cleaning pump can be controlled respectively by arranging the reagent needle inner wall cleaning valve and the sample needle inner wall cleaning valve.
In some embodiments, a sample needle cleaning liquid injection valve and a reagent needle cleaning liquid injection valve are respectively arranged on the outer wall of the sample cleaning tank and the pipeline of the outer wall of the reagent cleaning tank connected with the reagent sample outer wall cleaning pump. The sample cleaning tank and the reagent cleaning tank can be respectively controlled by arranging the sample needle cleaning liquid injection valve and the reagent needle cleaning liquid injection valve, and pipelines connected with the reagent sample outer wall cleaning pump.
In some embodiments, a sample needle cleaning waste suction valve and a reagent needle cleaning waste suction valve are respectively arranged on a pipeline connected with the waste liquid pump at the bottom of the sample cleaning tank and the bottom of the reagent cleaning tank. The waste liquid pump has the advantages that the sample cleaning pool and the reagent cleaning pool can be respectively controlled by arranging the sample needle cleaning waste suction valve and the reagent needle cleaning waste suction valve, and the pipelines connected with the waste liquid pump are respectively controlled by the sample cleaning pool and the reagent cleaning pool.
In some embodiments, the outer wall of the sample washing tank is connected to the strong wash conduit, and a sample strong wash pump is provided on the connecting conduit. The sample cleaning tank has the advantage that the sample cleaning tank can be subjected to strong cleaning.
In some embodiments, the reagent sample filling module further comprises a measurement waste needle connected to the waste pump and having a waste suction valve disposed on the connecting conduit. The method has the advantage that the waste liquid can be measured.
In some embodiments, each of the cleaning needle priming receiver, the pre-priming receiver, and the priming receiver is respectively joined to each of the tubes to which the waste tube is connected, and the waste collection pump is disposed on the joined tubes. The device has the advantages that the waste liquid collecting pump can be arranged to control the waste discharge of the cleaning and filling module and the excitation liquid filling module.
In some embodiments, the third end of the pre-excitation liquid three-way valve and the two pipelines respectively connected with the pre-excitation liquid containers are respectively provided with a pre-excitation liquid two-way valve, and the pipelines respectively connected with the third end of the excitation liquid three-way valve and the two excitation liquid containers are respectively provided with an excitation liquid two-way valve. The device has the advantages that the pre-excitation liquid two-way valve and the excitation liquid two-way valve are arranged to control the related pipelines of the excitation liquid filling module.
According to an aspect of the present invention, there is provided a detection analysis method applied to the above-mentioned full-automatic chemiluminescence immunoassay analyzer, comprising the steps of:
1) Pouring the sample needle, the reagent needle, each cleaning and pouring needle, the pre-excitation liquid pouring needle and the excitation liquid pouring needle, and discharging the gas in the liquid path;
2) Performing excitation liquid pouring, cleaning needle pouring, and pouring of a sample needle and a reagent needle;
3) The reaction cup is moved to the first waste liquid sucking needle, the peristaltic pump for sucking waste liquid in the reaction cup is controlled to suck the liquid out, the waste liquid sucking operation for the first time is completed, the reaction cup is moved to the first cleaning and filling needle, the three-way valve of the cleaning needle and the plunger pump of the cleaning needle corresponding to the reaction cup are controlled to suck the cleaning liquid in the cleaning liquid container into the reaction cup, and the first cleaning operation is completed;
4) Sequentially using a second waste liquid sucking needle to perform a second waste liquid sucking operation, using a second waste liquid sucking needle to perform a second cleaning operation, using a third waste liquid sucking needle to perform a third waste liquid sucking operation, using a third waste liquid sucking needle to perform a third cleaning operation, and using a fourth waste liquid sucking needle to perform a fourth waste liquid sucking operation;
5) The sample needle firstly sucks a section of air, then descends and detects the air to be below the liquid level of the sample, the sample plunger pump operates to suck the sample into the sample needle, the sample needle further sucks a section of air, the sample needle is moved to a reaction cup and the sample is added into the reaction cup, and then the inner wall cleaning pump and the outer wall cleaning pump of the reagent sample are opened to wash the sample needle and the sample cleaning pool;
6) The reagent needle firstly sucks a section of air, then descends to detect the condition that the reagent is below the liquid level, the reagent plunger pump operates to suck the reagent into the reagent needle, the reagent needle sucks a section of air again, the reagent needle moves to the reaction cup and adds the reagent into the reaction cup to react, and then the reagent sample inner wall cleaning pump and the reagent sample outer wall cleaning pump are opened to flush the reagent needle and the reagent cleaning pool.
In some embodiments, step 2) further comprises the steps of:
a) Opening the waste liquid collecting pump, controlling and operating the pre-excitation liquid three-way valve, the pre-excitation liquid two-way valves and the pre-excitation liquid plunger pump, pumping the pre-excitation liquid in the pre-excitation liquid containers and pouring the pre-excitation liquid into the pre-excitation liquid pouring receiver through the pre-excitation liquid filling needle, and controlling and operating the excitation liquid three-way valve, the excitation liquid two-way valves and the excitation liquid plunger pump, pumping the excitation liquid in the excitation liquid containers and pouring the excitation liquid into the excitation liquid pouring receiver through the excitation liquid filling needle;
b) Opening the waste liquid collecting pump, controlling and operating the three-way valve of each cleaning needle and the plunger pump of each cleaning needle, pumping out the cleaning liquid in the cleaning liquid container and filling the cleaning liquid into the filling liquid receiver of each cleaning needle through each cleaning filling needle;
c) And opening the waste liquid pump, controlling and operating the sample plunger pump, the reagent plunger pump and the reagent sample inner wall cleaning pump, and leading the cleaning liquid in the cleaning liquid container into the sample cleaning pool and the reagent cleaning pool through the sample needle and the reagent needle respectively.
It is beneficial to describe a specific method of operation of each step of performing the infusion of various substrates.
Drawings
FIG. 1 is a schematic diagram of a full-automatic chemiluminescence immunoassay analyzer according to one embodiment of the present invention;
FIG. 2 is a schematic view of the liquid container module shown in FIG. 1;
FIG. 3 is a schematic view of the cleaning waste module shown in FIG. 1;
FIG. 4 is a schematic diagram of the reagent sample injection module of FIG. 1;
FIG. 5 is a schematic view of the cleaning and priming module of FIG. 1;
fig. 6 is a schematic structural view of the excitation liquid filling module shown in fig. 1.
In the figure: the liquid container module 100, the wash waste liquid module 200, the reagent sample filling module 300, the wash filling module 400, the excitation liquid filling module 500, the waste liquid container 101, the strong wash liquid container 102, the wash liquid container 103, the waste liquid pipe 104, the strong wash liquid pipe 105, the wash liquid pipe 106, the waste liquid sucking needle 201, the waste liquid sucking peristaltic pump 202, the sample needle 301, the reagent needle 302, the sample washing tank 303, the reagent washing tank 304, the sample plunger pump 305, the reagent plunger pump 306, the reagent sample inner wall washing pump 307, the reagent sample outer wall washing pump 308, the waste liquid pump 309, the reagent needle inner wall washing valve 310, the sample inner wall washing valve 311, the sample needle washing injection valve 312, the reagent needle washing injection valve 313, the sample needle washing suction waste valve 314, the reagent needle washing waste valve 315, the sample strong wash pump 316, the measurement waste liquid needle 317, the waste liquid suction valve 318, the wash injection needle 401, the wash needle filling connector 402, the wash needle three-way valve 403, the wash needle plunger pump 404, the waste liquid collection pump 405, the pre-excitation liquid filling needle 501, the excitation liquid injection needle 502, the pre-excitation liquid connector 504, the pre-excitation liquid injector 503, the pre-excitation liquid injector valve 508, the pre-excitation liquid injector valve 509, the pre-excitation liquid injector valve 507, the pre-excitation liquid injector valve 510, the pre-excitation liquid injector valve 507, the pre-excitation liquid injector valve and the pre-connector valve 510, the pre-excitation liquid injector valve and the pre-excitation liquid injector valve.
Detailed Description
The invention is described in further detail below with reference to the accompanying drawings.
As shown in fig. 1, the analyzer mainly includes a liquid container module 100, a wash waste module 200, a reagent sample filling module 300, a wash filling module 400, and an excitation liquid filling module 500.
As shown in fig. 2, the liquid container module 100 includes a waste liquid container 101, a strong washing liquid container 102, and a washing liquid container 103, which are provided independently of each other. Wherein, the waste liquid container 101 is connected with a waste liquid pipeline 104, namely, waste liquid can be discharged into the waste liquid container 101 or can be pumped out from the waste liquid container 101 through the waste liquid pipeline 104; the strong washing liquid container 102 is connected with a strong washing liquid pipeline 105, namely strong washing liquid can be pumped out of the strong washing liquid container 102 through the strong washing liquid pipeline 105; the cleaning liquid container 103 is connected with a cleaning liquid pipe 106, that is, the cleaning liquid can be pumped out of the cleaning liquid container 103 through the cleaning liquid pipe 106.
As shown in fig. 3, the cleaning waste liquid module 200 includes a plurality of waste liquid sucking needles 201, wherein each waste liquid sucking needle 201 is connected to the waste liquid pipe 104 and a waste liquid sucking peristaltic pump 202 is provided on the connecting pipe, and the operation of the waste liquid sucking peristaltic pump 202 can suck the waste liquid in the waste liquid container 101 into each waste liquid sucking needle 201 through the waste liquid pipe 104 for the subsequent waste liquid cleaning work.
Preferably, the number of waste liquid sucking needles 201 is four.
As shown in fig. 4, the reagent sample filling module 300 mainly includes a sample needle 301, a reagent needle 302, a sample washing tank 303, a reagent washing tank 304, a sample plunger pump 305, a reagent plunger pump 306, and the like. One end of the sample needle 301 is located directly above the sample washing tank 303, the other end of the sample needle is connected to the sample plunger pump 305, one end of the reagent needle 302 is located directly above the reagent washing tank 304, and the other end of the reagent needle is connected to the reagent plunger pump 306. Further, the sample plunger pump 305 and the reagent plunger pump 306 are both connected to the cleaning liquid pipe 106, and a reagent sample inner wall cleaning pump 307 is provided on the connecting pipe. Thereby, under the operation control of the reagent sample inner wall cleaning pump 307, the sample plunger pump 305, the reagent plunger pump 306, and the like, the cleaning liquid in the cleaning liquid container 103 can be drawn out through the cleaning liquid pipe 106 and added to the sample cleaning reservoir 303 and the reagent cleaning reservoir 304 through the sample needle 301 and the reagent needle 302, respectively, to clean the sample cleaning reservoir 303 and the reagent cleaning reservoir 304.
Preferably, a sample needle inner wall cleaning valve 310 and a reagent needle inner wall cleaning valve 311 are respectively provided on the pipes connecting the sample plunger pump 305 and the reagent plunger pump 306 with the reagent sample inner wall cleaning pump 307, so as to further ensure the effect of operation control thereof.
The outer wall of the sample washing reservoir 303 and the outer wall of the reagent washing reservoir 304 are both connected to the washing liquid pipe 106, and a reagent sample outer wall washing pump 308 is provided on the connection pipe thereof. Thereby, under the operation control of the reagent sample outer wall cleaning pump 308, the cleaning liquid in the cleaning liquid container 103 can be pumped out through the cleaning liquid pipe 106 to clean the outer wall of the sample cleaning reservoir 303 and the outer wall of the reagent cleaning reservoir 304.
Preferably, a sample needle cleaning liquid injection valve 312 and a reagent needle cleaning liquid injection valve 313 are respectively arranged on the outer wall of the sample cleaning tank 303 and the outer wall of the reagent cleaning tank 304 and the pipeline connected with the reagent sample outer wall cleaning pump 308, so as to further ensure the operation control effect.
The bottom of the sample washing tank 303 and the bottom of the reagent washing tank 304 are both connected to the waste liquid pipe 104, and a waste liquid pump 309 is provided on the connection pipe thereof. Thereby, under the operation control of the waste liquid pump 309, the waste liquid in the sample washing tank 303 and the reagent washing tank 304 can be discharged into the waste liquid container 101 through the waste liquid pipe 104.
Furthermore, the outer wall of the sample washing tank 303 is connected to the strong washing liquid pipe 105, and a sample strong washing pump 316 is provided on the connection pipe. Thus, under the operation control of the sample strong washing pump 316, strong washing liquid in the strong washing liquid container 102 can be pumped out through the strong washing liquid pipe 105 to strong wash the outer wall of the sample washing tank 303.
Preferably, the reagent sample filling module 300 further comprises a measurement waste needle 317, the measurement waste needle 317 being connected to the waste pump 309 and a waste suction valve 318 being provided in the connecting line. Thus, by the operation of the waste liquid pump 309 and the waste suction valve 318, the waste liquid in the waste liquid container 101 can be drawn out through the waste liquid pipe 104 into the measurement waste liquid needle 317 for subsequent waste liquid measurement work.
As shown in fig. 5, the purge and fill module 400 mainly includes a plurality of purge and fill needles 401, a plurality of purge needle priming receptacles 402, a plurality of purge needle three-way valves 403, and a plurality of purge needle plunger pumps 404, which are the same in number. Wherein, one end of each cleaning filling needle 401 is respectively located right above each cleaning needle filling liquid receiver 402, the other end is respectively connected with the first end of each cleaning needle three-way valve 403, the second end of each cleaning needle three-way valve 403 is respectively connected with each cleaning needle plunger pump 404, and the third end is connected with the cleaning liquid pipeline 106. Thus, under the operation of the respective cleaning needle plunger pumps 404 and the control of the respective cleaning needle three-way valves 403, the cleaning liquid in the cleaning liquid container 103 can be drawn out through the cleaning liquid pipe 106 and filled into the respective cleaning needle filling liquid receiver 402 through the respective cleaning filling needles 401.
In addition, the bottom end of each of the cleaning needle priming connectors 402 is connected to the waste liquid pipe 104, and a waste liquid collecting pump 405 is provided on the connected pipe, so that the waste liquid collecting pump 405 can control the waste liquid in each of the cleaning needle priming connectors 402 to be discharged into the waste liquid container 101 through the waste liquid pipe 104.
Preferably, the number of purge fill needle 401, purge needle priming receptacle 402, purge needle three-way valve 403, and purge needle plunger pump 404 are all three.
As shown in fig. 6, the priming module 500 mainly includes a pre-priming needle 501, a priming needle 502, a pre-priming receiver 503, a priming receiver 504, a pre-priming three-way valve 505, a priming three-way valve 506, a pre-priming plunger pump 507, a priming plunger pump 508, two pre-priming containers 509, and two priming containers 510.
Wherein, one end of the pre-excitation liquid filling needle 501 is located right above the pre-excitation liquid filling liquid receiver 503, the other end is connected with the first end of the pre-excitation liquid three-way valve 505, the second end of the pre-excitation liquid three-way valve 505 is connected with the pre-excitation liquid plunger pump 507, and the third end is respectively connected with the two pre-excitation liquid containers 509. Thus, the pre-priming solution in the two pre-priming solution containers 509 can be filled into the pre-priming solution priming receiver 503 through the pre-priming solution filling needle 501 by operation of the pre-priming solution plunger pump 507 and control of the pre-priming solution three-way valve 505.
Preferably, the third end of the pre-excitation liquid three-way valve 505 is provided with a pre-excitation liquid two-way valve 511 on the pipeline respectively connected with the two pre-excitation liquid containers 509, so as to further ensure the control effect.
One end of the excitation liquid filling needle 502 is positioned right above the excitation liquid filling liquid receiver 504, the other end of the excitation liquid filling needle is connected with the first end of the excitation liquid three-way valve 506, the second end of the excitation liquid three-way valve 506 is connected with the excitation liquid plunger pump 508, and the third end of the excitation liquid three-way valve 506 is connected with two excitation liquid containers 510 respectively. Thus, the priming solution in the two priming reservoirs 510 may be filled into the priming receiver 504 through the priming needle 502 by operation of the priming plunger pump 508 and control of the priming three-way valve 506.
Preferably, the third end of the excitation liquid three-way valve 506 and the two excitation liquid containers 510 are respectively connected with two pipelines, and the excitation liquid two-way valve 512 is arranged on each pipeline to further ensure the control effect.
In addition, the bottom ends of the pre-priming connector 503 and the priming connector 504 are connected to the waste liquid conduit 104. Wherein, the pipes connected to the bottom end of each cleaning needle priming liquid receiver 402, the bottom end of the pre-priming liquid receiver 503 and the bottom end of the priming liquid receiver 504 are connected to the waste liquid pipe 104 after being combined, and the waste liquid collecting pump 405 is arranged on the combined pipes.
The detection and analysis method of the full-automatic chemiluminescence immunoassay analyzer comprises the following specific steps.
The first step is to start the device, and fill the sample needle 301, the reagent needle 302, the cleaning filling needles 401, the pre-excitation liquid filling needle 501, the excitation liquid filling needle 502 and other needles, so that the gas in the liquid path of each needle is discharged, and the redundant liquid in each needle flows into the liquid waste container, thereby avoiding corrosion caused by long-term contact with the liquid,
and secondly, pouring the substrate, wherein the substrate comprises a priming solution pouring step, a cleaning needle pouring step, a sample needle pouring step and a reagent needle pouring step.
In the process of priming, the waste liquid collecting pump 405 is firstly turned on to be in a working state until the priming is completely finished; then, the pre-priming or priming solution is poured into the pre-priming or priming solution pouring connector 503 or 504 as required, and then the excess pre-priming or priming solution is introduced into the waste liquid container 101 during pouring.
Taking the priming pre-priming solution as an example, the second end and the third end of the pre-priming solution three-way valve 505 are opened, the two-way valves 511 of each pre-priming solution are opened, the plunger of the pre-priming solution plunger pump 507 is operated to the bottom, the pre-priming solution in each pre-priming solution container 509 can be pumped out, then the first end of the pre-priming solution three-way valve 505 is switched to be opened, the two-way valves 511 of each pre-priming solution are closed, the plunger of the pre-priming solution plunger pump 507 is operated to the top, the pre-priming solution can be poured into the pre-priming solution pouring receiver 503 through the pre-priming solution filling needle 501, and finally the steps are repeated until a proper amount of pre-priming solution is poured.
The principle and method of priming is similar to priming pre-priming.
In the process of filling the cleaning needles, the waste liquid collecting pump 405 is also turned on first until the filling is completely finished, and then the cleaning liquid is filled into the cleaning needle filling liquid receiver 402 according to the requirement, wherein the principle and the method for filling the cleaning liquid are similar to that of filling the excitation liquid, namely, the cleaning liquid in the cleaning liquid container 103 is pumped out and filled into the cleaning needle filling liquid receiver 402 through the cleaning filling needles 401 by controlling and operating the cleaning needle three-way valves 403 and the cleaning needle plunger pumps 404, and the redundant cleaning liquid is introduced into the waste liquid container 101 during filling.
In the sample needle and reagent needle priming, the sample needle purge waste valve 314, the reagent needle purge waste valve 315, and the waste liquid pump 309 are opened to bring the sample purge tank 303 and the reagent purge tank 304 into a waste liquid collection state until the priming is completed. Then, the reagent needle inner wall cleaning valve 310 and the sample needle inner wall cleaning valve 311 are opened, and the sample plunger pump 305, the reagent plunger pump 306, and the reagent sample inner wall cleaning pump 307 are operated, so that the cleaning liquid in the cleaning liquid container 103 flows into the sample cleaning reservoir 303 and the reagent cleaning reservoir 304 through the sample needle 301 and the reagent needle 302, respectively. And excess cleaning liquid is introduced into the waste liquid container 101 during the priming.
And thirdly, cleaning the reaction cup.
Moving the reaction cup to the corresponding position of the first waste liquid sucking needle 201, and operating the peristaltic pump 202 for controlling waste liquid sucking to suck out the liquid in the reaction cup and leading the liquid into the waste liquid container 101 to finish the first waste liquid sucking operation; then the reaction cup is moved to the corresponding position of the first cleaning and filling needle 401, and the cleaning needle three-way valve 403 and the cleaning needle plunger pump 404 corresponding to the reaction cup are operated and controlled to suck the cleaning liquid in the cleaning liquid container 103 into the reaction cup, so that the first cleaning work is completed; then, the second waste liquid sucking needle 201 is used for sucking waste liquid for the second time, the second cleaning and filling needle 401 is used for completing the second cleaning work, the third waste liquid sucking needle 201 is used for sucking waste liquid for the third time, the third cleaning and filling needle 401 is used for completing the third cleaning work, and the fourth waste liquid sucking needle 201 is used for sucking waste liquid for the fourth time, so that the reaction cup cleaning process is completed.
Fourth, filling the sample and the reagent into the reaction cup.
Taking the sample filling as an example, the sample needle 301 firstly sucks a section of air, then descends to detect 1-2mm below the sample liquid surface, the sample plunger pump 305 operates to suck the sample into the sample needle 301 and lift the sample into the high position, and the sample needle 301 sucks a section of air again. The first section of air is used for avoiding the dilution of a sample by the cleaning liquid, the second section of air is used for ensuring that the sample liquid does not overflow outwards in the moving process, and the sampling accuracy is improved.
The sample needle 301 is moved to the cuvette and down to a fixed depth, and the sample is introduced into the cuvette and half of the air in the upper section of the sample is expelled. Then, the sample needle 301 is moved to above the sample washing reservoir 303, the reagent sample inner wall washing pump 307 and the reagent needle inner wall washing valve 310 are opened, the washing liquid in the washing liquid container 103 is sucked out to wash the inner wall of the sample washing reservoir 303, the reagent sample outer wall washing pump 308 and the sample needle washing liquid filling valve 312 are opened, the washing liquid is made to form a flowing water plane in the sample washing reservoir 303, and the sample needle 301 is lowered to 10mm below the water plane to wash.
Then, the reagent needle 302 and the reagent plunger pump 306 may be used to fill the reagent into the cuvette to perform a reaction, and the reagent needle 302 and the reagent washing tank 304 may be washed. The principle and flow of filling reagent and washing are similar to filling sample.
Furthermore, if the analysis detected is an infectious item, a strong wash of the sample wash tank 303 is also required. In the strong washing, the sample strong washing pump 316 is turned on to suck the strong washing liquid in the strong washing liquid container 102 into the sample washing tank 303, the sample plunger pump 305 is operated to repeatedly flow the strong washing liquid in the sample washing tank 303, so as to perform strong washing on the sample needle 301 and the sample washing tank 303, and then perform washing on the sample needle 301 and the sample washing tank 303.
What has been described above is merely some embodiments of the present invention. It will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the spirit of the invention.
Claims (7)
1. Full-automatic chemiluminescence immunoassay analyzer is characterized in that: comprises a liquid container module (100), a cleaning waste liquid module (200), a reagent sample filling module (300), a cleaning filling module (400) and an excitation liquid filling module (500);
the liquid container module (100) comprises a waste liquid container (101), a strong washing liquid container (102) and a washing liquid container (103), wherein the waste liquid container (101) is connected with a waste liquid pipeline (104), the Jiang Xiye container (102) is connected with a strong washing liquid pipeline (105), and the washing liquid container (103) is connected with a washing liquid pipeline (106);
the cleaning waste liquid module (200) comprises four waste liquid sucking needles (201), wherein each waste liquid sucking needle (201) is connected with the waste liquid pipeline (104) and a waste liquid sucking peristaltic pump (202) is arranged on the connecting pipeline;
the reagent sample filling module (300) comprises a sample needle (301), a reagent needle (302), a sample washing tank (303), a reagent washing tank (304), a sample plunger pump (305) and a reagent plunger pump (306), wherein one end of the sample needle (301) is positioned right above the sample washing tank (303), the other end of the sample needle is connected with the sample plunger pump (305), one end of the reagent needle (302) is positioned right above the reagent washing tank (304), the other end of the reagent needle is connected with the reagent plunger pump (306), the sample plunger pump (305) and the reagent plunger pump (306) are both connected with the washing liquid pipeline (106) and a reagent sample inner wall washing pump (307) is arranged on a connecting pipeline, the outer wall of the sample washing tank (303) and the outer wall of the reagent washing tank (304) are both connected with the washing liquid pipeline (106) and a reagent outer wall washing pump (308) is arranged on a connecting pipeline (308), the bottom of the sample washing tank (303) and the waste liquid pipeline (309) are both connected with a waste liquid pump (309), a sample needle inner wall cleaning valve (310) and a reagent needle inner wall cleaning valve (311) are respectively arranged on the pipelines connected with the sample plunger pump (305) and the reagent plunger pump (306) and the reagent sample inner wall cleaning pump (307);
the cleaning and filling module (400) comprises three cleaning and filling needles (401), three cleaning needle filling liquid receivers (402), three cleaning needle three-way valves (403) and three cleaning needle plunger pumps (404), wherein one end of each cleaning and filling needle (401) is respectively positioned right above each cleaning needle filling liquid receiver (402), the other end of each cleaning and filling needle is respectively connected with the first end of each cleaning needle three-way valve (403), the second end of each cleaning needle three-way valve (403) is respectively connected with each cleaning needle plunger pump (404), the third end of each cleaning needle three-way valve is connected with a cleaning liquid pipeline (106), and the bottom end of each cleaning needle filling liquid receiver (402) is connected with the waste liquid pipeline (104) and is provided with a waste liquid collecting pump (405) on a connecting pipeline;
the excitation liquid filling module (500) comprises a pre-excitation liquid filling needle (501), an excitation liquid filling needle (502), a pre-excitation liquid filling connector (503), an excitation liquid filling connector (504), a pre-excitation liquid three-way valve (505), an excitation liquid three-way valve (506), a pre-excitation liquid plunger pump (507), an excitation liquid plunger pump (508), two pre-excitation liquid containers (509) and two excitation liquid containers (510), wherein one end of the pre-excitation liquid filling needle (501) is positioned right above the pre-excitation liquid filling connector (503), the other end of the pre-excitation liquid filling needle is connected with a first end of the pre-excitation liquid three-way valve (505), a second end of the pre-excitation liquid three-way valve (505) is connected with the pre-excitation liquid plunger pump (507), third ends of the pre-excitation liquid three-way valve (506) are respectively connected with two pre-excitation liquid plunger pumps (507) and are respectively arranged on the connected pipeline, one end of the pre-excitation liquid filling needle (501) is positioned right above the pre-excitation liquid filling connector (503), the other end of the pre-excitation liquid three-way valve (506) is connected with the excitation liquid three-way valve (506), the other end of the pre-excitation liquid three-way valve (505) is connected with the pre-excitation liquid three-way valve (506), the bottom ends of the pre-excitation liquid filling liquid receiver (503) and the excitation liquid filling liquid receiver (504) are connected with the waste liquid pipeline (104), the cleaning needle filling liquid receiver (402), the pre-excitation liquid filling liquid receiver (503) and the excitation liquid filling liquid receiver (504) are respectively converged with the pipelines connected with the waste liquid pipeline (104), and the waste liquid collecting pump (405) is arranged on the converged pipeline.
2. The full-automatic chemiluminescence immunoassay analyzer of claim 1, wherein: the outer wall of the sample cleaning pool (303) and the outer wall of the reagent cleaning pool (304) are respectively provided with a sample needle cleaning liquid injection valve (312) and a reagent needle cleaning liquid injection valve (313) on a pipeline connected with the reagent sample outer wall cleaning pump (308).
3. The full-automatic chemiluminescence immunoassay analyzer of claim 1, wherein: the bottom of the sample cleaning pool (303) and the pipeline, which is connected with the waste liquid pump (309), of the reagent cleaning pool (304) are respectively provided with a sample needle cleaning waste suction valve (314) and a reagent needle cleaning waste suction valve (315).
4. The full-automatic chemiluminescence immunoassay analyzer of claim 1, wherein: the outer wall of the sample cleaning tank (303) is connected with the strong washing liquid pipeline (105), and a sample strong washing pump (316) is arranged on the connecting pipeline.
5. The full-automatic chemiluminescence immunoassay analyzer of claim 1, wherein: the reagent sample filling module (300) further comprises a measurement waste needle (317), wherein the measurement waste needle (317) is connected with the waste pump (309), and a waste suction valve (318) is arranged on the connecting pipeline.
6. A detection analysis method applied to the full-automatic chemiluminescence immunoassay analyzer of any one of claims 1-5, wherein: comprises the following steps
1) Filling the sample needle (301), the reagent needle (302), each of the cleaning filling needle (401), the pre-excitation liquid filling needle (501) and the excitation liquid filling needle (502), and discharging the gas in the liquid path;
2) Performing excitation liquid pouring, cleaning needle pouring, and pouring of a sample needle and a reagent needle;
3) The reaction cup is moved to the first waste liquid sucking needle (201), the peristaltic pump (202) for sucking out liquid in the reaction cup is operated and controlled to finish the first waste liquid sucking operation, the reaction cup is moved to the first cleaning and filling needle (401), and the three-way valve (403) of the cleaning needle and the plunger pump (404) corresponding to the reaction cup are operated and controlled to suck out the cleaning liquid in the cleaning liquid container (103) into the reaction cup to finish the first cleaning operation;
4) Sequentially using a second waste liquid sucking needle (201) to perform a second waste liquid sucking operation, using a second waste liquid sucking needle (401) to perform a second cleaning operation, using a third waste liquid sucking needle (201) to perform a third waste liquid sucking operation, using a third waste liquid sucking needle (401) to perform a third cleaning operation, and using a fourth waste liquid sucking needle (201) to perform a fourth waste liquid sucking operation;
5) The sample needle (301) firstly sucks a section of air, then descends to detect the condition that the sample is below the liquid level of the sample, the sample plunger pump (305) operates to suck the sample into the sample needle (301), the sample needle (301) further sucks a section of air, the sample needle (301) moves to a reaction cup and adds the sample into the reaction cup, and then the reagent sample inner wall cleaning pump (307) and the reagent sample outer wall cleaning pump (308) are opened to flush the sample needle (301) and the sample cleaning pool (303);
6) The reagent needle (302) firstly sucks a section of air, then descends to detect the condition that the reagent is below the liquid level, the reagent plunger pump (306) operates to suck the reagent into the reagent needle (302), the reagent needle (302) sucks a section of air again, the reagent needle (302) moves to a reaction cup and adds the reagent into the reaction cup to react, and then the reagent sample inner wall cleaning pump (307) and the reagent sample outer wall cleaning pump (308) are opened to flush the reagent needle (302) and the reagent cleaning pool (304).
7. The detection and analysis method applied to the full-automatic chemiluminescence immunoassay analyzer as set forth in claim 6, wherein: step 2) further comprises the following steps
A) Opening the waste liquid collecting pump (405), controlling to operate the pre-excitation liquid three-way valve (505) and the pre-excitation liquid plunger pump (507), pumping the pre-excitation liquid in each pre-excitation liquid container (509) and pouring the pre-excitation liquid into the pre-excitation liquid pouring receiver (503) through the pre-excitation liquid filling needle (501), and controlling to operate the excitation liquid three-way valve (506) and the excitation liquid plunger pump (508), pumping the excitation liquid in each excitation liquid container (510) and pouring the excitation liquid into the excitation liquid pouring receiver (504) through the excitation liquid filling needle (502);
b) Opening the waste liquid collection pump (405), controlling and operating the three-way valve (403) and the plunger pump (404) of each cleaning needle, and pumping out the cleaning liquid in the cleaning liquid container (103) and filling the cleaning liquid into the filling liquid receiver (402) of each cleaning needle through the cleaning filling needle (401);
c) And opening the waste liquid pump (309), controlling and operating the sample plunger pump (305), the reagent plunger pump (306) and the reagent sample inner wall cleaning pump (307), and leading the cleaning liquid in the cleaning liquid container (103) into the sample cleaning pool (303) and the reagent cleaning pool (304) through the sample needle (301) and the reagent needle (302), respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311136417.6A CN116859071B (en) | 2023-09-05 | 2023-09-05 | Full-automatic chemiluminescence immunoassay analyzer and detection analysis method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311136417.6A CN116859071B (en) | 2023-09-05 | 2023-09-05 | Full-automatic chemiluminescence immunoassay analyzer and detection analysis method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116859071A CN116859071A (en) | 2023-10-10 |
| CN116859071B true CN116859071B (en) | 2023-11-10 |
Family
ID=88236353
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311136417.6A Active CN116859071B (en) | 2023-09-05 | 2023-09-05 | Full-automatic chemiluminescence immunoassay analyzer and detection analysis method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116859071B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111257579A (en) * | 2020-03-23 | 2020-06-09 | 厦门优迈科医学仪器有限公司 | Full-automatic chemiluminescence immunoassay analyzer |
| CN211179865U (en) * | 2019-11-30 | 2020-08-04 | 威海威高生物科技有限公司 | Liquid path device for chemiluminescence immunity analyzer |
| CN113514656A (en) * | 2021-05-13 | 2021-10-19 | 桂林优利特医疗电子有限公司 | High-speed full-automatic chemiluminescence immunoassay analyzer liquid path system |
| CN215005450U (en) * | 2021-05-13 | 2021-12-03 | 桂林优利特医疗电子有限公司 | High-speed full-automatic chemiluminescence immunoassay analyzer liquid path system |
| CN114776269A (en) * | 2022-04-15 | 2022-07-22 | 长江大学 | Experimental device and method for simulating influence of cracks on oil displacement effect of nano-magnetic fluid |
| CN114813520A (en) * | 2022-04-11 | 2022-07-29 | 中国科学院苏州生物医学工程技术研究所 | Flow cytometer liquid path system and flow cytometer |
-
2023
- 2023-09-05 CN CN202311136417.6A patent/CN116859071B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN211179865U (en) * | 2019-11-30 | 2020-08-04 | 威海威高生物科技有限公司 | Liquid path device for chemiluminescence immunity analyzer |
| CN111257579A (en) * | 2020-03-23 | 2020-06-09 | 厦门优迈科医学仪器有限公司 | Full-automatic chemiluminescence immunoassay analyzer |
| CN113514656A (en) * | 2021-05-13 | 2021-10-19 | 桂林优利特医疗电子有限公司 | High-speed full-automatic chemiluminescence immunoassay analyzer liquid path system |
| CN215005450U (en) * | 2021-05-13 | 2021-12-03 | 桂林优利特医疗电子有限公司 | High-speed full-automatic chemiluminescence immunoassay analyzer liquid path system |
| CN114813520A (en) * | 2022-04-11 | 2022-07-29 | 中国科学院苏州生物医学工程技术研究所 | Flow cytometer liquid path system and flow cytometer |
| CN114776269A (en) * | 2022-04-15 | 2022-07-22 | 长江大学 | Experimental device and method for simulating influence of cracks on oil displacement effect of nano-magnetic fluid |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116859071A (en) | 2023-10-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101324487A (en) | Quantitative sampling and dilution analysis apparatus | |
| CN111721956A (en) | Liquid path system of full-automatic biochemical analyzer | |
| CN112798345A (en) | Pre-dilution mode sample collection and distribution system, method and blood cell analyzer | |
| JP2008518240A (en) | Method and apparatus for automating sample loading in liquid chromatography | |
| CN215711746U (en) | Reagent filling device | |
| CN112462082A (en) | Automatic sample adding system and automatic sample adding method | |
| CN113075013A (en) | Solution preparation device and preparation method thereof and in-vitro diagnosis and analysis equipment | |
| CN110146708B (en) | Sample adding system and method based on speed open loop control and stroke positioning compensation | |
| CN116859071B (en) | Full-automatic chemiluminescence immunoassay analyzer and detection analysis method thereof | |
| CN111024481A (en) | Sample mixing method and sample analyzer | |
| CN201083641Y (en) | Liquid analytical equipment | |
| CN112557687A (en) | Integrated liquid path control system, control method and integrated biochemical analyzer | |
| CN112748254A (en) | Medium system for sample detection equipment | |
| CN201965136U (en) | Flow path system for automation biochemical reaction flow | |
| CN210720437U (en) | Sample adding needle cleaning liquid drainage system for in-vitro diagnostic equipment | |
| CN217652869U (en) | Quantitative assembly, sample processing device and reagent preparation device | |
| CN215641281U (en) | Liquid path system of biological sample detection analyzer and biological sample detection analyzer | |
| CN209946178U (en) | Liquid path system of full-automatic biochemical analyzer | |
| CN209858577U (en) | Liquid path system | |
| CN219831102U (en) | Full-automatic dry-type fluorescence immunoassay appearance | |
| CN102632057B (en) | Cleaning device for capillary tube of automatic sampler and cleaning method | |
| CN215677713U (en) | Pipettor cleaning system and sample analyzer | |
| CN209858317U (en) | Full-automatic dilution liquid preparation device | |
| JP2663661B2 (en) | Liquid vacuum suction device | |
| CN217155955U (en) | Large-circulation chemical examination sampling system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |