CN220610452U - Flushable reagent taking and placing device and cell enrichment and dyeing integrated equipment - Google Patents

Abstract

The utility model discloses a flushable reagent taking and placing device and cell enrichment and dyeing integrated equipment, wherein the reagent taking and placing device comprises: a reagent sucking and discharging assembly for sucking/discharging the reagent; the intermediate container is communicated with the reagent sucking and discharging assembly; the two-way pump is arranged between the intermediate container and the reagent sucking and discharging assembly; a rinse liquid supply unit for supplying a rinse liquid into the intermediate container; when the intermediate container is not used for storing flushing liquid, the bidirectional pump can convey negative pressure or positive pressure to the reagent sucking and discharging assembly, and the reagent sucking and discharging assembly is controlled to suck or discharge the reagent; after the washing liquid supply assembly supplies washing liquid to the intermediate container, the bidirectional pump can convey the washing liquid from the intermediate container to the reagent sucking and discharging assembly for washing. In the utility model, the bidirectional pump can control the suction and discharge of the reagent, and can control the delivery of the flushing liquid to the reagent suction and discharge assembly for flushing, so that the reagent suction and discharge assembly is not required to be detached for cleaning independently, and the operation is more convenient.

Description

Flushable reagent taking and placing device and cell enrichment and dyeing integrated equipment

Technical Field

The utility model belongs to the technical field of medical instruments, and particularly relates to a flushable reagent taking and placing device and cell enrichment and dyeing integrated equipment.

Background

In most detection in the biomedical field, the same or different reagents are required to be added into a sample to be detected repeatedly for many times, and in most detection processes at present, detection personnel are required to manually add the reagents, so that the efficiency bias is greatly influenced. The existing device capable of automatically adding the reagent can only realize the throwing of a single type of reagent, and when the reagent type needs to be replaced, the device needs to be disassembled and cleaned, so that the device is very troublesome.

Therefore, if the reagent taking and placing device with the cleaning function can be designed, the automatic addition of different types of reagents can be realized, the detection efficiency is obviously improved, and the reagent taking and placing device plays an important role in various detection and research in the field of biological medicine. For example, the efficiency of immunological research, genetic research or microbiological detection can be improved, and even the research and development of biochips, and organoids can be facilitated. On the other hand, the reagent taking and placing device with the cleaning function can be suitable for changing various eluents to realize the application scene of a chromatographic separation preparation method, or can be also applied to the application scene of adding samples or reagents in a timed and quantitative manner, such as the induction expression of a gene recombinant and the purification of a product.

In the biomedical field, for example, circulating tumor cells are detected by taking a blood sample from a patient, and then processing the blood sample to enrich the circulating tumor cells therein and stain the cells for further detection. In the process of enriching and staining cells, different reagents need to be added into a blood sample for many times, and if the addition of the reagents can only be completed through manual operation, the detection efficiency can be seriously affected, and even the diagnosis and subsequent treatment of a patient can be delayed. Even if the existing device capable of automatically adding the reagent is adopted for assisting the detection process, detection personnel are required to frequently detach and clean the device, and under the condition of large sample quantity, the problem that the detection result cannot be obtained in time still occurs.

In view of this, the present utility model has been made.

Disclosure of Invention

The utility model aims to solve the technical problems of overcoming the defects of the prior art and providing a flushable reagent taking and placing device and a cell enrichment and dyeing integrated device, which can realize the internal flushing without disassembly and are convenient to use.

In order to solve the technical problems, the utility model adopts the basic conception of the technical scheme that:

a flushable reagent dispensing device comprising:

a reagent sucking and discharging assembly for sucking/discharging the reagent;

the intermediate container is communicated with the reagent sucking and discharging assembly;

the bidirectional pump is arranged between the intermediate container and the reagent sucking and discharging assembly;

a rinse liquid supply assembly for supplying a rinse liquid into the intermediate container;

when the intermediate container is not filled with flushing liquid, the bidirectional pump can convey negative pressure or positive pressure to the reagent sucking and discharging assembly, and the reagent sucking and discharging assembly is controlled to suck or discharge reagent;

after the washing liquid supply assembly supplies washing liquid to the intermediate container, the bidirectional pump can convey the washing liquid from the intermediate container to the reagent sucking and discharging assembly for washing.

Further, the reagent sucking and discharging assembly comprises a reagent accommodating tube for temporarily storing the sucked reagent; the upper end opening of the reagent holding tube is communicated with the two-way pump through a pipeline.

Further, the reagent sucking and discharging assembly further comprises a needle, the needle is communicated with the lower end opening of the reagent accommodating tube, and the reagent is sucked into or discharged out of the reagent accommodating tube through the needle.

Further, the washing liquid supply assembly includes:

a washing liquid storage bottle for storing washing liquid;

a washing liquid conveying pipeline, the inlet end of which is communicated with the washing liquid storage bottle, and the outlet end of which is communicated with the intermediate container;

and a transfer pump for transferring the rinse liquid in the rinse liquid storage bottle to the intermediate container.

Further, the inlet end of the wash liquid delivery line is disposed proximate to the bottom of the wash liquid storage bottle.

Further, the reagent taking and placing device comprises a plurality of reagent sucking and placing components and a plurality of bidirectional pumps which are arranged in one-to-one correspondence with the plurality of reagent sucking and placing components; and the two-way pumps are communicated with the same intermediate container.

Further, the intermediate container is connected with a main pipe, the main pipe is respectively communicated with a plurality of shunt pipes, and each bidirectional pump is connected with the shunt pipes in a one-to-one correspondence manner.

Further, the intermediate container is a lotion buffer bottle, and one end of the main pipe extends into the bottom of the lotion buffer bottle.

Further, the bidirectional pump is a peristaltic pump, one end of the peristaltic pump is communicated with the reagent sucking and discharging assembly through a pipeline, and the other end of the peristaltic pump is communicated with the intermediate container through a pipeline.

The integrated cell enrichment and staining equipment comprises the flushable reagent taking and placing device.

By adopting the technical scheme, compared with the prior art, the utility model has the following beneficial effects.

In the utility model, the bidirectional pump can control the suction and discharge of the reagent and can control the delivery of the flushing liquid to the reagent suction and discharge assembly for flushing, so that the device has multiple purposes and simplifies the structure of the reagent suction and discharge device. Through setting up the washing liquid supply assembly, can carry the washing liquid to the intermediate container again before need wash, and when not needing to wash, does not deposit the washing liquid in the intermediate container, and then reagent is inhaled and is put the subassembly and can be conducted with the atmosphere through the intermediate container, does not influence the two-way pump and utilizes the pressure differential change to control the process that reagent was absorbed and discharged. In the use, need not to pull down reagent and inhale and put the subassembly and wash alone, more convenient operation.

In the utility model, the upper end opening of the reagent accommodating tube is communicated with the bi-directional pump, the lower end opening is connected with the needle through which the reagent passes in and out, and when the bi-directional pump conveys flushing liquid into the reagent accommodating tube for flushing, the flushing liquid enters from the upper end opening of the reagent accommodating tube, enters into the needle through the lower end opening and is finally discharged from the needle. Under the action of gravity, the flushing liquid can flush the pipe wall of the reagent accommodating pipe more fully, so that a more thorough and effective flushing effect is realized.

The following describes the embodiments of the present utility model in further detail with reference to the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the utility model. It is evident that the drawings in the following description are only examples, from which other drawings can be obtained by a person skilled in the art without the inventive effort. In the drawings:

FIG. 1 is a schematic view of a reagent dispensing apparatus (reagent sucking/discharging process) according to a first embodiment of the present utility model;

FIG. 2 is a schematic view of a reagent dispensing device (rinsing process) according to a first embodiment of the present utility model;

FIG. 3 is a schematic diagram of a reagent dispensing device according to a second embodiment of the present utility model.

In the figure: 110. a reagent sucking and discharging assembly; 111. a reagent accommodating tube; 114. the rubber tube is communicated; 115. a needle; 116. a support frame; 130. a reagent holding device; 140. an intermediate container; 141. a bi-directional pump; 142. a header pipe; 143. a shunt; 144. a first communication pipe; 145. a second communicating pipe; 146. a wash buffer bottle; 150. a wash liquid supply assembly; 151. a wash liquid delivery line; 152. a transfer pump; 153. the washing liquid is stored in the bottle.

It should be noted that these drawings and the written description are not intended to limit the scope of the inventive concept in any way, but to illustrate the inventive concept to those skilled in the art by referring to the specific embodiments.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present utility model, and the following embodiments are used to illustrate the present utility model, but are not intended to limit the scope of the present utility model.

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

Example 1

As shown in fig. 1 and 2, this embodiment provides a reagent that can wash gets puts device, can realize the suction and the discharge of reagent to realize the function of automatic interpolation reagent, simultaneously, reagent gets and puts device and still can realize independently washing, has saved the trouble that the manual dismantlement of operating personnel was cleared up.

Specifically, the reagent dispensing device of the present embodiment includes a reagent dispensing assembly 110, an intermediate container 140, a bi-directional pump 141, and a wash liquid supply assembly 150. Wherein the reagent sucking and discharging assembly 110 is used for sucking or discharging the reagent, the intermediate container 140 is communicated with the reagent sucking and discharging assembly 110, and the bi-directional pump 141 is arranged between the intermediate container 140 and the reagent sucking and discharging assembly 110. The rinse solution supply assembly 150 communicates with the intermediate container 140 for supplying rinse solution into the intermediate container 140.

In this embodiment, when the reagent pick-and-place apparatus sucks and discharges the reagent, the intermediate container 140 does not store the rinse liquid as shown in fig. 1. At this time, the bi-directional pump 141 delivers negative pressure to the reagent sucking and discharging unit 110, that is, the reagent sucking and discharging unit 110 extracts air and reduces pressure, and the reagent sucking and discharging unit 110 can suck the required reagent by using the internal negative pressure environment. After the reagent is sucked, the bi-directional pump 141 conveys positive pressure to the reagent sucking and discharging assembly 110, that is, ventilates and pressurizes the reagent sucking and discharging assembly 110, and the reagent sucking and discharging assembly 110 can discharge the reagent sucked inside due to the pressure increase, so that the reagent adding process is completed.

When the reagent sucking and discharging unit 110 needs to be flushed, as shown in fig. 2, the flushing liquid supplying unit 150 supplies the flushing liquid into the intermediate container 140, and a certain amount of the flushing liquid is stored in the intermediate container 140. The bi-directional pump 141 is then operated to transfer the rinse liquid from the intermediate container 140 to the reagent suction and discharge assembly 110 for rinsing.

In the above-described scheme, by providing the intermediate container 140, when the rinse liquid is not stored therein, the bi-directional pump 141 can be directly connected to the atmosphere through the intermediate container 140, so that the negative or positive pressure can be supplied to the reagent suction and discharge assembly 110. When the flushing is required, the flushing liquid is injected into the intermediate container 140, and the flushing operation of the reagent sucking and discharging assembly 110 can be completed through the bi-directional pump 141. Thus, the suction and discharge of the reagent and the transfer of the rinse liquid to the reagent suction and discharge assembly 110 can be controlled by the bi-directional pump 141, simplifying the structure of the reagent suction and discharge device. Meanwhile, the reagent taking and placing device can be controlled to switch between two working states of sucking and discharging the reagent and flushing the reagent sucking and placing component 110 by whether flushing liquid is stored in the intermediate container 140 or not, and the device is not required to be disassembled for flushing, so that the operation is more convenient.

In a further aspect of this embodiment, the reagent sucking and discharging assembly 110 includes a reagent accommodating tube 111 for temporarily storing the sucked reagent. The reagent holding tube 111 is disposed vertically, and its upper end opening is in communication with the bi-directional pump 141 through a pipe.

Further, the reagent sucking and discharging assembly 110 further includes a needle 115, the needle 115 communicates with the lower end opening of the reagent containing tube 111, and the reagent is sucked into or discharged from the reagent containing tube 111 through the needle 115.

Specifically, in the present embodiment, the bi-directional pump 141 is a peristaltic pump, one end of which is in communication with the reagent containing tube 111 of the reagent sucking and discharging assembly 110 through the second communication tube 145, and the other end of which is in communication with the intermediate container 140 through the first communication tube 144. More specifically, a communicating hose 114 is connected to the lower end opening of the reagent containing tube 111, and the lower end of the communicating hose 114 is connected to a needle 115.

With the above structure, when the reagent taking and placing device is in operation, the lower end of the needle 115 is immersed below the liquid level of the reagent to be sucked, and then the reagent can be sucked into the reagent accommodating tube 111 along the needle 115 by sucking air from the reagent accommodating tube 111 and decompressing the air by the peristaltic pump. Then, the peristaltic pump is used to ventilate and pressurize the reagent containing tube 111, so that the reagent in the tube can be discharged along the needle 115, thereby adding the reagent.

When the reagent sucking and discharging assembly 110 needs to be flushed, the flushing liquid sequentially passes through the first communication pipe 144, the peristaltic pump and the second communication pipe 145 under the pumping action of the peristaltic pump, enters the pipe from the upper end of the reagent containing pipe 111, then flows out from the lower end and finally is discharged through the needle 115. The flushing liquid flows downwards in the reagent containing tube 111 under the action of gravity, so that the tube wall of the reagent containing tube 111 can be flushed more fully, and a more thorough and effective flushing effect is realized.

Further, in this embodiment, the intermediate container 140 is a lotion buffer bottle 146, and the first communication tube 144 extends into the bottle from the mouth of the lotion buffer bottle 146. One end of the first communication pipe 144 extending into the washing liquid buffer bottle 146 is disposed at a position close to the bottom of the washing liquid buffer bottle 146, preferably against the bottom of the washing liquid buffer bottle 146. In this way, the rinse solution supplied from the rinse solution supply assembly 150 to the rinse solution cache bottle 146 can be sufficiently pumped along the first communication tube 144 by the bi-directional pump 141 to avoid residual rinse solution in the rinse solution cache bottle 146.

In a further aspect of this embodiment, the lotion supply assembly 150 includes a lotion storage bottle 153, a lotion delivery line 151, and a delivery pump 152. The washing liquid storage bottle 153 is used for storing washing liquid, an inlet end of the washing liquid conveying pipeline 151 is communicated with the washing liquid storage bottle 153, an outlet end of the washing liquid conveying pipeline 151 is communicated with the washing liquid caching bottle 146, and the conveying pump 152 is used for conveying washing liquid in the washing liquid storage bottle 153 to the washing liquid caching bottle 146 along the washing liquid conveying pipeline 151.

It will be appreciated that the wash solution storage bottle 153 and the wash solution buffer bottle 146 in this embodiment are not sealed, but may be communicated with the outside atmosphere, ensuring that the sucking and discharging operations of the reagent and the transfer process of the wash solution are smoothly performed.

In one embodiment of the present embodiment, a transfer pump 152 is provided on the wash liquid transfer line 151, and a peristaltic pump is employed as the transfer pump 152. In use, the peristaltic pump used as the transfer pump 152 controls the volume of the rinse solution transferred from the rinse solution storage bottle 153 to the rinse solution buffer bottle 146, so that the rinse solution entering the rinse solution buffer bottle 146 can be completely consumed in the subsequent rinsing process, and the subsequent process of sucking and discharging the reagent is not affected.

Further, in the present embodiment, the inlet end of the lotion feed line 151 extends into the lotion storage bottle 153 from the mouth of the bottle, and is disposed near the bottom of the lotion storage bottle 153. Preferably, the inlet end of the wash liquid delivery line 151 is in contact with the bottom of the wash liquid storage bottle 153.

With the above structure, the rinse liquid stored in the rinse liquid storage bottle 153 can be completely extracted, and the condition that the rinse liquid remains in the rinse liquid storage bottle 153, but the inlet end of the rinse liquid conveying pipeline 151 is separated from the liquid surface of the rinse liquid, and the rinse liquid cannot be continuously extracted is avoided. In this way, after the operator adds the rinse solution to the rinse solution storage bottle 153 once, the added rinse solution can be fully utilized to complete the multiple rinsing processes, thereby avoiding the trouble of adding the rinse solution frequently.

In a further aspect of this embodiment, the reagent sucking and discharging assembly 110 is mounted on a moving mechanism (not shown in the figure) and can move in both horizontal and vertical directions under the driving of the moving mechanism. A plurality of reagent holding apparatuses 130, such as test tubes, for holding reagents are disposed under the reagent sucking and holding assembly 110, and the reagents held in the respective reagent holding apparatuses 130 may be different. The reagent sucking and placing assembly 110 is driven to move by controlling the moving mechanism, so that the reagent can be sucked from the corresponding reagent holding device 130, and then the reagent is moved to the position above the position where the reagent needs to be added, and the sucked reagent is discharged, so that the reagent adding operation is completed.

The operation of the reagent dispensing device according to this embodiment will be described in detail below.

Before using the reagent dispensing device, an operator needs to add a sufficient amount of washing liquid to the washing liquid storage bottle 153, and also needs to place one to a plurality of reagent storage devices 130 storing reagents. And then the reagent taking and placing device can be started to perform reagent adding operation.

After the reagent taking and placing device is started, the reagent sucking and placing assembly 110 is driven by the moving mechanism to move to the position above the reagent containing device 130 where the required reagent is stored, and then the reagent sucking and placing assembly 110 is driven to descend, so that the lower end of the needle 115 is immersed in the reagent. Then, the motor of the bi-directional pump 141 is rotated forward to provide negative pressure, so that the reagent can be sucked into the reagent accommodating tube 111. After the reagent is sucked, the moving mechanism drives the reagent sucking and placing assembly 110 to rise until the lower end of the needle 115 is higher than the top opening of the reagent holding device 130, and then drives the reagent sucking and placing assembly 110 to horizontally move to a position above the position where the reagent needs to be added. After the reagent sucking and discharging assembly 110 is moved in place, the motor of the bi-directional pump 141 is reversed to provide positive pressure, so that the reagent in the reagent accommodating tube 111 can be discharged along the needle 115, and a reagent adding operation is completed.

After reagent addition is completed, if a different reagent needs to be sucked for addition, the reagent sucking and discharging assembly 110 is rinsed first. Specifically, the transfer pump 152 is started to transfer the rinse liquid to be consumed for one rinse operation into the rinse liquid cache bottle 146. During or after the transfer of the rinse solution to the rinse solution buffer bottle 146, the moving mechanism is required to drive the reagent sucking and discharging assembly 110 to move above a waste solution collecting container (not shown) for collecting the waste solution. Then, the motor of the bi-directional pump 141 is reversed to convey the rinse liquid in the rinse liquid buffer bottle 146 to the reagent sucking and discharging unit 110, and the rinse liquid sequentially flows through the reagent holding tube 111, the communicating hose 114 and the needle 115 from top to bottom, and finally is discharged along the lower end of the needle 115 to be collected in the waste liquid collecting container. After the washing is completed, the reagent can be added again.

The above reagent adding operation and the operation of flushing the reagent sucking and discharging assembly 110 are alternately performed, and may be repeated a plurality of times, thereby realizing the addition of a plurality of different reagents to satisfy different detection requirements. The reagent adding operation and the flushing operation can be automatically controlled in a preset program mode, so that the reagent taking and placing device can complete the adding process of various reagents in a full-automatic mode without manual intervention, and pollution can not exist among different reagents. The operator only needs to pre-place the reagent containing device 130 and add sufficient flushing liquid, so that the subsequent detection process can be directly performed after the reagent containing device is operated.

Example two

As shown in fig. 3, this embodiment is further defined in the first embodiment, and the reagent picking and placing device includes a plurality of reagent sucking and placing components 110, and a plurality of bi-directional pumps 141 disposed in one-to-one correspondence with the plurality of reagent sucking and placing components 110. Several bi-directional pumps 141 are in communication with the same intermediate container 140, i.e. the same wash buffer bottle 146.

Specifically, a plurality of second communication pipes 145 are provided, and the plurality of reagent containing tubes 111 are communicated with the plurality of bi-directional pumps 141 in one-to-one correspondence through the plurality of second communication pipes 145.

In this embodiment, by providing a plurality of reagent sucking and discharging units 110, a plurality of reagent adding operations can be performed simultaneously. For example, in the detection process, reagents can be added to a plurality of samples at the same time, so that the method is suitable for rapid detection of a large number of samples. The reagent sucking and discharging assemblies 110 are respectively connected with a two-way pump 141 in a one-to-one correspondence manner, so that whether each reagent sucking and discharging assembly 110 performs sucking/discharging operation of the reagent or not and whether flushing is required or not can be independently controlled and are not interfered with each other. Therefore, the number of the used reagent sucking and discharging components 110 can be selected according to the actual sample number, and other reagent sucking and discharging components 110 which do not need to be used do not need to be detached from the reagent taking and discharging device, so that the reagent sucking and discharging device is convenient to use and can be more flexibly suitable for different application scenes.

In this embodiment, the two-way pumps 141 are connected to the same wash buffer bottle 146, so that the washing requirement can be met, and the overall structure of the reagent taking and placing device can be simplified.

In a further aspect of this embodiment, the wash buffer bottle 146 is connected to a manifold 142, the manifold 142 is respectively connected to a plurality of shunt tubes 143, and each bi-directional pump 141 is connected to a plurality of shunt tubes 143 in a one-to-one correspondence. When the bi-directional pump 141 starts to pump the flushing liquid in the flushing liquid cache bottle 146, the flushing liquid firstly enters the main pipe 142, then can be split along the main pipe 142, enters the split pipe 143 of the connected bi-directional pump 141 in a working state, finally enters the corresponding reagent accommodating pipe 111, and the flushing of the reagent sucking and discharging assembly 110 is realized.

Specifically, the manifold 142 and the plurality of shunt tubes 143 may be connected by a three-way joint or the like, thereby achieving respective communication of the manifold 142 and the plurality of shunt tubes 143.

Further, one end of the manifold 142 extends into the bottom of the wash buffer bottle 146, preferably against the bottom of the wash buffer bottle 146. In this manner, rinse solution entering the rinse solution cache bottle 146 may be completely drawn along the manifold 142 without the problem of rinse solution remaining in the rinse solution cache bottle 146.

In this embodiment, the plurality of reagent sucking and discharging assemblies 110 are fixed on one supporting frame 116, the supporting frame 116 is connected with a moving mechanism, and the moving mechanism can drive the supporting frame 116 to move, so that the plurality of reagent sucking and discharging assemblies 110 move synchronously.

The specific working process of the reagent picking and placing device in this embodiment is as follows.

After the reagent taking and placing device is started, the moving mechanism drives the supporting frame 116 to move, so that one or more reagent sucking and placing components 110 move to the position above the corresponding reagent containing device 130, and then drives the supporting frame 116 to descend, so that the lower end of the needle 115 in the reagent sucking and placing component 110 requiring reagent sucking is immersed in the reagent. Then, the corresponding one or more bi-directional pumps 141 deliver negative pressure, sucking the reagent into the reagent accommodating tube 111. After the reagent is sucked, the moving mechanism drives the supporting frame 116 to rise until the lower ends of the needles 115 are higher than the top opening of the reagent holding device 130, and then drives the supporting frame 116 to move horizontally, so that the reagent sucking and placing assembly 110 storing the reagent moves to a position above the position where the reagent needs to be added. After moving into place, the corresponding one or more bi-directional pumps 141 deliver positive pressure, and the reagent is discharged from the reagent containing tube 111, completing the addition of the reagent.

After the reagent addition is completed, the reagent suction and discharge assembly 110 is rinsed. The transfer pump 152 is started to transfer the rinse liquid to be consumed for one rinse operation into the rinse liquid cache bottle 146. In general, the greater the number of reagent suction and discharge assemblies 110 that need to be flushed, the greater the volume of flushing fluid delivered. The moving mechanism also needs to drive the supporting frame 116 to move, so that the reagent sucking and discharging assembly 110 moves above a waste liquid collecting container (not shown in the figure) for collecting waste liquid. Then, the corresponding one or more bi-directional pumps 141 are activated to deliver the rinse solution to the reagent suction and discharge assembly 110 to be rinsed, thereby completing the rinsing process.

In another working mode of the reagent taking and placing device, the moving mechanism drives the supporting frame 116 to move, so that at least two reagent sucking and placing components 110 are located above the reagent containing device 130 storing different reagents, and then drives the supporting frame 116 to descend, so that the lower ends of the needles 115 of the at least two reagent sucking and placing components 110 are immersed in the reagents. Then, the corresponding at least two bi-directional pumps 141 simultaneously supply negative pressure, and different reagents are sucked into the respective reagent accommodating tubes 111.

Then, the moving mechanism drives the supporting frame 116 to rise first and then move horizontally, so as to move the at least one reagent sucking and discharging assembly 110 to a position above the position where the reagent needs to be added. Then, the corresponding at least one bi-directional pump 141 is operated to deliver positive pressure to add reagent to the corresponding location. After the addition is completed, the moving mechanism drives the support frame 116 to move horizontally again, the reagent sucking and discharging assembly 110 sucked with the other reagent is moved to the same position, and then the corresponding bidirectional pump 141 conveys positive pressure, so that the other reagent can be added to the same position.

The above procedure may be repeated a number of times to sequentially add a plurality of different reagents to the same location, e.g., the same test sample. Thus, the support 116 is driven to repeatedly move without a moving mechanism to suck the reagent, so that the efficiency is improved. After all the reagents to be added are added, the reagent sucking and discharging assembly 110 is flushed, and at this time, the plurality of reagent sucking and discharging assemblies 110 which have just sucked different reagents can be flushed simultaneously, that is, the corresponding bidirectional pumps 141 are started simultaneously, and flushing fluid is delivered to the plurality of reagent sucking and discharging assemblies 110.

The reagent taking and placing device of the embodiment is provided with a plurality of reagent sucking and placing components 110, so that a plurality of reagent adding operations can be completed simultaneously, or a plurality of different reagents can be sucked at one time and added sequentially, thereby being beneficial to improving the efficiency of reagent adding.

Example III

As shown in fig. 1 to 3, the present embodiment further provides a cell enrichment and staining integrated apparatus, which includes the flushable reagent taking and placing device described in the first or second embodiment.

Specifically, the cell enrichment and staining integrated device can autonomously convey a blood sample collected from a patient, and filter the blood sample to enrich nucleated pathological cells. The reagent taking and placing device can add various reagents into the blood sample, thereby assisting the enrichment process of cells and staining the enriched cells for the subsequent detection flow. In the process of adding the reagent, if different reagents are required to be sequentially sucked by the same reagent sucking and discharging assembly 110 for adding, after the addition of one reagent is completed, the conveying pump 152 can be started to convey quantitative flushing liquid into the flushing liquid buffer bottle 146, then the flushing liquid is conveyed to the reagent sucking and discharging assembly 110 for flushing through the two-way pump 141, and after the flushing is completed, the next reagent is sucked. The reagent taking and placing device and the device for filtering the blood sample and staining the cells are all arranged on the same fixed frame to form an integrated device capable of realizing the whole process of cell enrichment and staining.

The foregoing description is only illustrative of the preferred embodiment of the present utility model, and is not to be construed as limiting the utility model, but is to be construed as limiting the utility model to any and all simple modifications, equivalent variations and adaptations of the embodiments described above, which are within the scope of the utility model, may be made by those skilled in the art without departing from the scope of the utility model.

Claims (10)

1. A flushable reagent dispensing device, comprising:

a reagent sucking and discharging assembly for sucking/discharging the reagent;

the intermediate container is communicated with the reagent sucking and discharging assembly;

the bidirectional pump is arranged between the intermediate container and the reagent sucking and discharging assembly;

a rinse liquid supply assembly for supplying a rinse liquid into the intermediate container;

when the intermediate container is not filled with flushing liquid, the bidirectional pump can convey negative pressure or positive pressure to the reagent sucking and discharging assembly, and the reagent sucking and discharging assembly is controlled to suck or discharge reagent;

after the washing liquid supply assembly supplies washing liquid to the intermediate container, the bidirectional pump can convey the washing liquid from the intermediate container to the reagent sucking and discharging assembly for washing.

2. The flushable reagent dispensing device of claim 1, wherein the reagent aspirating assembly comprises a reagent receiving tube for storing aspirated reagent; the upper end opening of the reagent holding tube is communicated with the two-way pump through a pipeline.

3. The flushable reagent dispensing device of claim 2, wherein the reagent aspirating assembly further comprises a needle in communication with the lower end opening of the reagent containing tube through which reagent is aspirated or expelled from the reagent containing tube.

4. A flushable reagent dispensing device according to any one of claims 1 to 3, wherein the wash liquid supply assembly comprises:

a washing liquid storage bottle for storing washing liquid;

a washing liquid conveying pipeline, the inlet end of which is communicated with the washing liquid storage bottle, and the outlet end of which is communicated with the intermediate container;

and a transfer pump for transferring the rinse liquid in the rinse liquid storage bottle to the intermediate container.

5. The flushable reagent dispensing device of claim 4, wherein the inlet end of the wash liquid delivery line is positioned adjacent the bottom of the wash liquid storage bottle.

6. A flushable reagent dispensing device according to any one of claims 1 to 3, wherein the reagent dispensing device comprises a plurality of reagent dispensing components and a plurality of bi-directional pumps disposed in one-to-one correspondence with the plurality of reagent dispensing components; and the two-way pumps are communicated with the same intermediate container.

7. The flushable reagent pick-and-place device of claim 6, wherein the intermediate container is connected with a manifold, the manifold is respectively communicated with a plurality of shunt tubes, and each bi-directional pump is connected with a plurality of shunt tubes in a one-to-one correspondence.

8. The flushable reagent dispensing device of claim 7, wherein the intermediate container is a wash buffer bottle and one end of the manifold extends into the bottom of the wash buffer bottle.

9. A flushable reagent dispensing device according to any one of claims 1 to 3, wherein the bi-directional pump is a peristaltic pump having one end in communication with the reagent dispensing assembly via a conduit and the other end in communication with the intermediate container via a conduit.

10. A cell enrichment staining apparatus comprising the flushable reagent delivery device of any of claims 1 to 9.