CN113358435A - Sample loading device and analysis and sorting equipment comprising same - Google Patents
Sample loading device and analysis and sorting equipment comprising same Download PDFInfo
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- CN113358435A CN113358435A CN202110652413.8A CN202110652413A CN113358435A CN 113358435 A CN113358435 A CN 113358435A CN 202110652413 A CN202110652413 A CN 202110652413A CN 113358435 A CN113358435 A CN 113358435A
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- 238000012360 testing method Methods 0.000 claims abstract description 111
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- 238000013519 translation Methods 0.000 description 4
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- 230000015572 biosynthetic process Effects 0.000 description 1
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- 238000010276 construction Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Abstract
The invention relates to a sample loading device and analysis and sorting equipment comprising the same, wherein the sample loading device comprises a test tube carrier, a first driving device, a bin body and a sample feeding device, the test tube carrier for bearing a test tube can be driven by the first driving device to be used for enabling the test tube to extend into or out of the hollow part, and when the test tube is positioned in the hollow part, a sample liquid in the test tube is transferred from the sample feeding device to a flow chamber through a power source. The invention also comprises analysis and sorting equipment, wherein the analysis and sorting equipment comprises the sample loading device, the flow chamber, the detection module, the control module and the sorting module. The invention adopts the power source to realize positive pressure to drive the sample liquid to be transferred to the flow chamber so as to realize automatic sample loading of the sample liquid, and the degree of automation is high. Through increasing liquid way mechanism, can guarantee the constant temperature environment of storehouse body hollow part to guarantee that the test tube can keep the activity of biological particle in the sample liquid after getting into hollow part, with the analysis sorting performance that improves analysis sorting facilities, reduce cost.
Description
Technical Field
The invention relates to the field of analysis and sorting equipment, in particular to a sample loading device and analysis and sorting equipment comprising the same.
Background
The analyzing and sorting equipment is a device for automatically analyzing and sorting cells, can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in a liquid, and can sort out specified cell subsets according to a preselected parameter range.
At present, the existing sorting, analyzing and sorting equipment has a complex structure, and cannot realize the uniform mixing of samples and control the temperature of a sample liquid in the sorting process, so that the activity of biological particles in the sample liquid cannot be maintained, and the replacement of test tubes after the samples are sorted is complicated, time-consuming and labor-consuming, and has low automation degree, so a sample loading device related to the sorting and analyzing equipment needs to be designed.
Disclosure of Invention
In view of the above-mentioned shortcomings of the prior art, it is an object of the present invention to provide a loading device and an analytical sorting apparatus including the same, which solve one or more of the problems of the prior art.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a sample loading device, which comprises
A test tube carrier for carrying a test tube;
a first driving device connected with the test tube carrier for driving the test tube carrier to move;
the device comprises a bin body and a control device, wherein the bin body is provided with a hollow part and a passage connected with the hollow part, and the passage is used for leading a power source to the hollow part;
the sample feeding device is provided with a hollow part partially extending into the bin body;
bear the test tube carrier accessible of test tube the drive of first drive arrangement to be used for realizing the test tube stretches into or stretches out hollow portion, the test tube is in hollow portion through the power supply with the sample liquid in the test tube by sampling device shifts to the flow chamber.
Further, the sample loading device is also provided with a second driving device, the second driving device is provided with a part connected with a test tube holder, and the test tube holder is used for connecting the test tube so as to drive the test tube to rotate through the second driving device to realize uniform mixing of the sample liquid.
Furthermore, the sample loading device further comprises a liquid path mechanism arranged at the hollow part of the bin body, the liquid path mechanism comprises a pipeline arranged close to the inner wall of the bin body, and the pipeline is provided with a pipeline inlet and a pipeline outlet so as to be used for the inflow and outflow of constant-temperature liquid from the pipeline.
Furthermore, the outer side of the bin body is covered by a cover body, and one part of the cover body is connected with the cover plate, so that the bin body is sealed in the cover body.
Further, the sample loading device further comprises an illuminating device, and the illuminating device is arranged on the hollow part.
Further, the sample loading device further comprises a cleaning mechanism, one part of the cleaning mechanism is wrapped on the sample feeding device, and the other part of the cleaning mechanism is communicated with the cleaning liquid inflow port.
Furthermore, the sample loading device also comprises a waste liquid collector, wherein the test tube carrier is provided with a circulation hole, and the waste liquid collector is provided with a waste liquid outflow port connected with the circulation hole.
Further, the sample loading device further comprises a door, and the door can be arranged on the door frame in an opening and closing mode.
Further, the sample loading device further comprises a third driving device, one part of the third driving device is connected with the first driving device, and the other part of the third driving device is connected with the door.
Furthermore, the sample loading device also comprises a reset mechanism, one part of the reset mechanism is arranged on the door frame, and the other part of the reset mechanism is arranged on the door, so that the door can be closed through the reset mechanism after being opened.
Furthermore, the sample loading device further comprises a magnetic suction mechanism, one part of the magnetic suction mechanism is arranged on the door frame, and the other part of the magnetic suction mechanism is arranged on the door.
The analysis and sorting equipment comprises the sample loading device and a flow chamber connected with the sample loading device, and further comprises a detection module, a control module and a sorting module, wherein a sample liquid is transferred to the flow chamber from the sample loading device, the detection module is used for detecting and acquiring at least one characteristic of a biological particle in the sample liquid, the control module sorts and sorts the liquid drops according to the at least one characteristic of the biological particle in the liquid drops, and the sorting module places the liquid drops into a specified position by utilizing the sorting and sorting of the liquid drops.
Compared with the prior art, the invention has the following beneficial technical effects:
the sample liquid is driven to be transferred to the flow chamber in a positive pressure mode by adopting the power source, so that the sample liquid is automatically loaded, the use is convenient, and the automation degree is high.
And (II) further, by adding a liquid path mechanism, the constant temperature environment of the hollow part of the bin body can be ensured, so that the activity of biological particles in the sample liquid can be kept after the test tube enters the hollow part, the analysis and sorting performance of the analysis and sorting equipment is improved, and the cost is reduced.
And (III) further, the outer wall of the sample feeding device can be washed by adding a cleaning mechanism, so that the carrying rate of pollution or bacteria is reduced, and the analysis and separation performance of the analysis and separation equipment is improved.
(IV) furtherly, hold in the palm through setting up second drive arrangement and test tube and can realize the rotation to the test tube to guarantee the homogeneous mixing of sample liquid in the test tube, prevent in the sample liquid biological particle deposit to test tube bottom, select separately the performance in order to improve the analysis of analysis sorting facilities.
(V) furtherly, can realize test tube carrier, test tube and waste liquid collector's translation motion through setting up third drive arrangement for the test tube moves and can realize quick replacement outdoors, and waste liquid collector translation is to sampling device under simultaneously, the waste liquid treatment and the recovery of being convenient for.
(six) furtherly, can realize the automatic start-stop of door through setting up the connecting rod to guarantee to change the test tube on the test tube carrier, the contained angle that forms between the direction of motion of connecting rod and second cylinder sets up and can guarantee not to interfere with the AND gate formation when the test tube carrier is driven simultaneously, in order to improve the life of door.
And (seventhly), the reset mechanism can adapt to the closing speed of the door, can be automatically and slowly closed when the door is opened at a certain angle, reduces the impact force, forms a comfortable effect during closing, and prolongs the service life of the door.
Drawings
Fig. 1 shows an isometric view of a loading device and an analytical sorting apparatus incorporating the same according to an embodiment of the present invention.
Fig. 2 shows a cross-sectional view of a loading device in an embodiment of the present invention and an analytical sorting apparatus including the loading device.
Fig. 3 is a schematic bottom view of a sample loading device and a cartridge body in an analysis sorting device comprising the same according to an embodiment of the present invention.
Fig. 4 shows a schematic sectional structure view of the cartridge body in the direction a-a in the sample loading device and the analysis and sorting equipment comprising the same according to the embodiment of the invention.
Fig. 5 shows a schematic sectional structure view of the cartridge body in the C-C direction in the sample loading device and the analysis and sorting equipment comprising the same in the embodiment of the invention.
Fig. 6 shows a schematic sectional structure view of the cartridge body in the direction B-B in the sample loading device and the analysis sorting equipment comprising the same according to the embodiment of the invention.
Fig. 7 is a schematic view showing a partial enlarged structure of a sample loading device and a cabin body in the analysis and sorting equipment comprising the same according to the embodiment of the invention.
FIG. 8 shows a schematic diagram of a loading device and a pipeline in an analytical sorting device comprising the loading device according to an embodiment of the present invention.
FIG. 9 is a schematic diagram showing the relationship between the tube carrier, the door and the connecting rod in the sample loading device and the analytical sorting equipment comprising the device according to the embodiment of the present invention.
Fig. 10 is a schematic structural diagram of a door, a test tube carrier and a first driving device in a second sample loading device and an analysis and sorting device comprising the same according to an embodiment of the present invention.
In the drawings, the reference numbers: 1. a first driving device; 101. a first cylinder; 102. a first piston; 103. a first cylinder mount; 2. a test tube carrier; 201. a flow-through hole; 3. a second driving device; 301. a stepping motor; 302. a test tube carrier; 4. a test tube; 5. a bin body; 501. a hollow portion; 502. an illumination device; 503. a passage; 504. a liquid path inlet pipe; 505. a liquid path outlet pipe; 6. a sample introduction device; 701. a door; 702. a connecting rod; 703. a first damping bush; 704. a second damping bush; 705. a rotating shaft; 706. magnetically attracting the door; 707. frame magnetic attraction; 708. a door frame; 8. a third driving device; 801. a second cylinder; 802. a second piston; 803. a fixed block; 804. a first slider; 805. a first guide rail; 9. a cleaning mechanism; 901. a cleaning liquid inflow port; 902. cleaning the tube; 10. a liquid path mechanism; 1001. a cover plate; 1002. a pipeline; 10021. a pipeline inlet; 10022. a pipeline outlet; 1003. a cover body; 10031. a through hole; 11. a sealing device; 12. a support; 13. a waste liquid collector; 131. a waste liquid inlet; 132. a waste liquid outflow port; 14. a base plate; 15. a bin body mounting bracket; 16. a supporting seat; 171. a second guide rail; 172. and a second slider.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the sample loading device and the analytical sorting device including the same according to the present invention are further described in detail below with reference to the accompanying drawings and the detailed description. The advantages and features of the present invention will become more apparent from the following description. It is to be noted that the drawings are in a very simplified form and are all used in a non-precise scale for the purpose of facilitating and distinctly aiding in the description of the embodiments of the present invention. To make the objects, features and advantages of the present invention comprehensible, reference is made to the accompanying drawings. It should be understood that the structures, ratios, sizes, and the like shown in the drawings and described in the specification are only used for matching with the disclosure of the specification, so as to be understood and read by those skilled in the art, and are not used to limit the implementation conditions of the present invention, so that the present invention has no technical significance, and any structural modification, ratio relationship change or size adjustment should still fall within the scope of the present invention without affecting the efficacy and the achievable purpose of the present invention.
The first embodiment is as follows:
referring to fig. 1, the sample loading device according to an embodiment of the present invention includes a first driving device 1, a test tube carrier 2, a chamber 5, and a sample feeding device 6, wherein the first driving device 1 is connected to the test tube carrier 2, the test tube carrier 2 is used for carrying a test tube 4, a sample liquid can be placed inside the test tube 4, and the sample feeding device 6 is disposed in the chamber 5.
Referring to fig. 1 and 2, the following first describes a specific structure of the first driving device 1 as follows:
the first driving device 1 comprises a first air cylinder 101, a piston rod of the first air cylinder 101 is connected with a first piston 102, the first air cylinder 101 can drive the piston rod and the first piston 102 connected with the piston rod to move up and down along the vertical direction after ventilation, and the movable stroke of the piston rod driven by the first air cylinder 101 is 100 mm.
Of course, in other embodiments of the present invention, the first driving device 1 may also have any driving structure other than an air cylinder, such as an electric cylinder, and the driving direction of the first air cylinder 101 may be changed, and is not limited to moving up and down in the vertical direction as described in the present invention, as long as it can drive the test tube carrier 2 to move, for example, the first driving device 1 may be replaced with a structure as in the second embodiment, which can realize the linear motion of the test tube carrier 2 in a non-vertical direction, and the driving manner of the first driving device 1 in the second embodiment is described in detail in the second embodiment.
With continuing reference to fig. 1 and fig. 2, the sample loading device further includes a second driving device 3, in the sample loading device according to an embodiment of the present invention, the second driving device 3 employs any one of a stepping motor or a servo motor, an output end of the second driving device 3 is connected to the test tube holder 302, the test tube holder 302 has a hole for matching with the test tube 4, and the second driving device 3 can drive the test tube holder 302 and the test tube 4 to intermittently rotate, so as to achieve uniform mixing of the sample liquid in the sample test tube 4, and prevent biological particles in the sample liquid from depositing on the bottom of the test tube, so as to improve the analysis and sorting performance of the analysis and sorting apparatus.
Further, a sealing device 11 is further matched on the outer ring of the test tube holder 302, the sealing device 11 may be an O-ring, and the bottom of the O-ring is connected with the upper surface of the test tube carrier 2.
Further, in the sample loading device according to the first embodiment of the present invention, the rotation speed of the second driving device 3 may reach 0rpm to 300rpm or 0rpm to 600rpm or even higher, which is not further limited by the present invention.
With continued reference to fig. 1 and 2, the following describes the particular construction of the cartridge body 5:
the whole bin body 5 is arranged on a bin body mounting bracket 15, the bin body mounting bracket 15 is connected with a bottom plate 14, a hollow part 501 is arranged in the bin body 5, one part of the sample feeding device 6 is arranged in the hollow part 501, and the other part of the sample feeding device 6 is exposed out of the bin body 5. A passage 503 is formed in the cartridge body 5 and is communicated with the hollow portion 501, the passage 503 is used for introducing a power source to the hollow portion 501, and specifically, the power source in the sample loading device according to the first embodiment of the present invention is compressed gas.
Further, referring to fig. 2, fig. 3 and fig. 4, the sample loading device further includes a liquid path mechanism 10, and the liquid path mechanism 10 is used for introducing constant temperature water and ensuring that the hollow portion 501 inside the bin body 5 is at a constant temperature, so that the test tube 4 can be influenced by a constant temperature environment when the hollow portion 501 is at the constant temperature state, and the sample liquid is at the constant temperature state.
Further, with continuing reference to fig. 2, 3 and 4, the liquid path mechanism 10 includes a pipeline 1002 disposed near the inner wall of the bin body 5, the pipeline 1002 is bent in a square wave shape and surrounds in a ring shape, the head end of the pipeline 1002 has a pipeline inlet 10021, the tail end of the pipeline 1002 has a pipeline outlet 10022, the pipeline inlet 10021 is close to the pipeline outlet 10022, and the constant temperature liquid flows in from the pipeline inlet 10021 and flows out from the pipeline outlet 10022.
Further, with continuing reference to fig. 2, 3 and 4, the outer side of the bin body 5 is covered by a cover 1003, so that the interior of the bin body 5 can be further insulated, and the temperature range of the insulation can reach 4-50 ℃ to prevent the generation of condensed water on the metal surface. The upper end face of the cover 1003 is connected with a cover 1001 in a sealing manner, the joint between the cover 1001 and the cover 1003 is sealed by a sealing ring, so that the bin 5 is sealed in the inner space of the cover 1003, the inner space can be a counter bore arranged on the cover 1003, the shape of the counter bore is matched with that of the bin 5, similarly, a through hole 10031 is further formed in the contact position between the cover 1003 and the bottom of the bin 5, and the test tube 4 can extend into the hollow part 501 of the bin 5 through the through hole 10031.
Further, a liquid path inlet pipe 504 and a liquid path outlet pipe 505 are respectively disposed on the cover plate 1001, wherein the liquid path inlet pipe 504 is connected to the liquid path inlet 10021, the liquid path outlet pipe 505 is connected to the liquid path outlet 10022, and the constant temperature water flows from the liquid path inlet pipe 504 to the liquid path inlet 10021 and enters the liquid path 1002, and then flows out from the liquid path outlet 10022 to the liquid path outlet pipe 505.
Further, the cover plate 1001 and the cover body 1003 are made of a plastic material, such as any one of POM, ABS and PSU, the bin 5 is made of a metal material with good thermal conductivity and corrosion resistance, such as any one of 6061-T6 or 304 or 316L with an anodized surface, wherein the cover body 1003 covers the bin 5 to reduce the heat conduction inside and outside the bin 5, so that the temperature of the hollow part 501 of the bin 5 is controlled to be close to and maintained at a constant temperature through the liquid path mechanism 10.
Further, an illuminating device 502 is further arranged in the hollow part 501, and the illuminating device 502 is used for illuminating the hollow part 501 so as to improve the visibility of the cabin body 5 and facilitate observation by an operator. In the sample loading device according to the first embodiment of the present invention, the illumination device 502 employs an LED lamp.
Of course, in other embodiments of the present invention, the illumination device 502 may be other illumination devices, as long as the ambient brightness of the hollow portion 501 in the bin 5 can be improved, and the illumination devices are not limited to the LED lamp of the present invention.
Further, with reference to fig. 3, the sample loading device further includes a cleaning mechanism 9, specifically, the cleaning mechanism 9 is a cleaning tube 902 into which a cleaning liquid can be introduced, the cleaning tube 902 has a portion covering the outer side of the sample loading device 6, the cleaning tube 902 has another portion communicating with the cleaning liquid inlet 901, the other portion of the cleaning tube 902 includes at least one branch tube, in the first sample loading device according to the first embodiment of the present invention, the branch tube extends from the cover plate 1001 and is connected to the cleaning liquid inlet 901, the cleaning liquid driven by positive pressure flows into the cleaning tube 902 along the branch tube after flowing from the cleaning liquid inlet 901, and the cleaning liquid is attached to the outer side of the sample loading device 6 in the cleaning tube 902 to perform high-speed washing, so as to achieve the purpose of cleaning the sample loading device 6.
Referring to fig. 1 and 2, the sample loading device further includes a waste liquid collector 13, the waste liquid collector 13 has a waste liquid inlet 131 and a waste liquid outlet 132, the waste liquid collector 13 is fixed on the test tube carrier 2, the test tube carrier 2 is provided with a through hole 201, and the waste liquid outlet 132 of the waste liquid collector 13 flows out of the through hole 201.
With continuing reference to fig. 1 and fig. 2, the sample loading device further includes a third driving device 8, wherein the third driving device 8 has a portion connected to the first driving device 1 for driving the first driving device 1 and the test tube carrier 2 connected to the first driving device 1 to move through the third driving device 8.
With continued reference to fig. 1 and fig. 2, in detail, the first cylinder mounting seat 103 is respectively fixed on the left side and the right side of the first cylinder 101 in the first driving device 1. The third driving device 8 includes a second cylinder 801, a piston rod of the second cylinder 801 is connected to a second piston 802, the second piston 802 is fixedly connected to a portion of a fixed block 803, and another portion of the fixed block 803 is connected to the first cylinder mounting seat 103. At least one first sliding block 804 is fixedly connected to the bottom of each first cylinder mounting seat 103, and the first sliding block 804 is connected to a first guide rail 805, wherein the first guide rail 805 is connected to the base plate 14. Further, in the sample loading device according to the first embodiment of the present invention, the movable stroke of the piston rod driven by the second air cylinder 801 is 70 mm.
Of course, in other embodiments of the present invention, the third driving device 8 may also have any driving structure other than an air cylinder, such as an electric cylinder, and the driving direction of the second air cylinder 801 may be changed, and is not limited to the horizontal translation described in the present invention, as long as it is sufficient to drive the first driving device 1 and the test tube carrier 2 to move.
With continuing reference to fig. 1 and fig. 2, the sample loading device further includes a connecting rod 702, one end of the connecting rod 702 is connected to the fixing block 803, and the other end of the connecting rod 702 is connected to the back of the door 701, specifically, the door is close to the test tube carrier 2 and the test tube 4 for opening and closing and allowing a user to replace the test tube 4, and the third driving device 8 can drive the connecting rod 702 to drive the door 701 to open and close.
Further, with continuing reference to fig. 1, fig. 2 and fig. 9, in order to avoid the interference of the door 701 during the movement of the test tube carrier 2, the connecting rod 702 is connected to the fixed block 803 at an angle, specifically, the angle α between the connecting rod 702 and the horizontal axis of the fixed block 803 is an included angle α, and the included angle α has the following relationship with the width of the test tube carrier 2 and the distance between the test tube carrier 2 and the door 701:
wherein said L1Indicating the linear length of the connecting rod, L2Indicating the length of the horizontal projection, L, of the connecting rod3Indicating the length of the link in vertical projection, L4Denotes the distance, L, from the outside of the test tube carrier to the door5Indicating the width of the door.
As can be seen from the above relational expression, the larger the width of the test tube carrier 2, the smaller the angle α between the horizontal axes of the connecting rod 702 and the fixed block 803, the smaller the distance between the test tube carrier 2 and the door 701, and the smaller the angle between the connecting rod 702 and the fixed block 803 (i.e., the moving direction of the second cylinder 801). In the sample loading device according to the first embodiment of the present invention, the included angle α is 8 °.
The sample loading device of the first embodiment of the invention has the following specific working process:
referring to fig. 1 and fig. 2, first, the test tube 4 filled with the sample liquid is mounted on the test tube holder 302, and the stepping motor 301 is started to drive the test tube holder 302 and the test tube 4 to rotate, so that the sample liquid in the test tube 4 can be uniformly mixed in the rotating process, thereby improving the analysis and sorting efficiency of the subsequent analysis and sorting equipment.
With continued reference to fig. 1 and 2, when sample loading starts, the first cylinder 101 is started, the piston rod of the first cylinder 101 retracts from bottom to top and drives the first piston 102 and the test tube carrier 2 connected to the first piston 102 to move integrally from bottom to top, so that the test tube 4 moves from bottom to top and enters the hollow part 501 of the silo body 5 through the through hole in the cover 1003 until the sealing device 11, i.e. the O-ring, contacts with the outer side of the cover 1003, so that the test tube carrier 2 is in sealed connection with the cover 1003, the hollow part 501 is ensured to be sealed, and the power source is prevented from flowing out.
A power source, i.e., compressed gas, is injected into the hollow part 501 through the passage 503, and the sample liquid in the test tube 4 is driven by the compressed gas to flow from the sample introduction device 6, i.e., the inner bore of the sample introduction needle, into the flow chamber, thereby completing automatic sample introduction.
With continued reference to fig. 1 and 2, after the loading is finished, the passage 503 is communicated with the outside atmosphere to release the compressed gas in the hollow portion 501, and then the first cylinder 101 drives the first piston 102 to reset and separate the test tube carrier 2 from the cartridge body 5, i.e. the test tube 4 is separated from the hollow portion 501 of the cartridge body 5.
With continued reference to fig. 1 and 2, when the test tube needs to be replaced, the second cylinder 801 is activated and drives the second piston 802 to extend out horizontally, the second piston 802 drives the fixing block 803 and the first cylinder mounting seat 103, the first cylinder 101 and the test tube carrier 2 connected to the fixing block 803 to move in a translation manner integrally, and since the fixing block 803 is connected to the connecting rod 702, the door 701 connected to the connecting rod 702 can also be opened automatically, when the test tube carrier 2 and the test tube 4 move to a position close to the door 701 and stop, the waste liquid collector 13 is located right below the hollow portion 501 and the sample injection device 6, the cleaning process is started, specifically, the cleaning liquid flows into the cleaning tube 902 from the cleaning liquid inlet, the cleaning liquid flows along the wall of the cleaning tube 902 and adheres to the outer wall of the sample injection needle for high-speed washing, and the cleaned waste liquid flows into the waste liquid outlet 132 from the waste liquid inlet 131 of the waste liquid collector 13, finally, the waste liquid is led out from the circulation hole 201 of the test tube carrier 2 to an external waste liquid tank.
When the sample loading is needed again, the second air cylinder 801 is started again and controls the test tube carrier 2 and the first driving device 1 to move backwards integrally, and the sample loading can be carried out again until the test tube 4 on the test tube carrier 2 is located under the bin body 5.
Correspondingly, the invention also provides an analysis and sorting device, which comprises the sample loading device and a flow chamber connected with the sample loading device, and further comprises a detection module, a control module and a sorting module, wherein the sample loading device transfers the liquid drops in the sample liquid to the flow chamber, the detection module is used for detecting and acquiring at least one characteristic of biological particles in the liquid drops, the control module sorts and sorts the liquid drops according to the at least one characteristic of the biological particles in the liquid drops, and the sorting module places the liquid drops into a specified position by using the sorting and sorting of the liquid drops.
Example two:
the structure and working process of the second embodiment are mostly the same as those of the first embodiment, and the differences are as follows:
referring to fig. 10, in order to realize that a part of the test tube carrier 2 is not suspended and installed stably, in the second embodiment of the present invention, the first driving device 1 does not drive the test tube carrier to move vertically, the first driving device 1 drives the test tube carrier 2 to move horizontally, and the tail of the test tube carrier 2 is connected to the output end of the first driving device 1, preferably, the first driving device 1 specifically adopts an air cylinder and is arranged horizontally, wherein the bottom of the first driving device 1 is provided with a plurality of supporting seats 16 to ensure the stability of the first driving device 1. A pair of second guide rails 171 are connected to the bottom of the test tube carrier 2, each of the second guide rails 171 is slidably connected to a plurality of second sliders 172, and the test tube carrier 2 and the second guide rails 171 are driven by the piston of the air cylinder to linearly move with respect to the second sliders 172.
Further, with continued reference to fig. 10, in order to facilitate the door 701 to be opened with respect to the doorframe 708 and to enable the door 701 to be tightly connected to the doorframe 708 after being closed, a frame magnet 707 is disposed on the doorframe 708, a door magnet 706 is disposed on the door 701, and the door magnet 706 can be attracted to the frame magnet 707. In order to ensure that the door 701 can be opened after being contacted by the test tube carrier 2 and that the door 701 can be automatically reset after being opened, the loading device is further provided with at least one reset mechanism. The resetting mechanism comprises a pair of first damping shaft sleeves 703 arranged on the door 701 and a pair of second damping shaft sleeves 704 arranged on the door frame 708, wherein each first damping shaft sleeve 703 corresponds to the installation position of the second damping shaft sleeve 704, and the adjacent first damping shaft sleeves 703 and the second damping shaft sleeves 704 are connected through a rotating shaft 705. The damping shaft sleeve can adapt to the closing speed of the door 701, the opening degree of the door starts to automatically and slowly close at a certain angle (for example, 60 degrees) by utilizing the buffering technology, the impact force is reduced, the comfortable effect during closing is achieved, the door can be closed softly even if the door is closed hard, and the service life of the door is prolonged.
Specifically, with continued reference to fig. 10, after the first driving device 1 drives the test tube carrier 2 to perform a linear motion in the horizontal direction and contact the door 701, the door 701 is forced to rotate relative to the rotating shaft 705 through the first damping bushing 703, so that the door 701 is turned outwards relative to the door frame 708, thereby opening the door 701 and facilitating the user to replace the test tube. Similarly, when the first driving device 1 drives the test tube carrier 2 to perform the linear motion reset, the door 701 is reset through the first damping bushing 703 after being stressed no longer, so that the door 701 is closed relative to the door frame 708.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (12)
1. Sample loading device, its characterized in that: the sample loading device comprises
A test tube carrier for carrying a test tube;
a first driving device connected with the test tube carrier for driving the test tube carrier to move;
the device comprises a bin body and a control device, wherein the bin body is provided with a hollow part and a passage connected with the hollow part, and the passage is used for leading a power source to the hollow part;
the sample feeding device is provided with a hollow part partially extending into the bin body;
bear the test tube carrier accessible of test tube the drive of first drive arrangement to be used for realizing the test tube stretches into or stretches out hollow portion, the test tube is in hollow portion through the power supply with the sample liquid in the test tube by sampling device shifts to the flow chamber.
2. The loading device of claim 1, wherein: the sample loading device is also provided with a second driving device, the second driving device is provided with a part which is connected with a test tube holder, and the test tube holder is used for connecting the test tube so as to drive the test tube to rotate to realize uniform mixing of sample liquid through the second driving device.
3. The loading device of claim 1, wherein: the sample loading device further comprises a liquid path mechanism arranged in the hollow part of the bin body, the liquid path mechanism comprises a pipeline which is arranged close to the inner wall of the bin body, and the pipeline is provided with a pipeline inlet and a pipeline outlet and used for enabling constant-temperature liquid to flow in and out of the pipeline.
4. A loading unit as claimed in claim 3, wherein: the outer side of the bin body is covered by a cover body, and one part of the cover body is connected with the cover plate, so that the bin body is sealed in the cover body.
5. A loading unit as claimed in claim 3, wherein: the sample loading device further comprises an illuminating device, and the illuminating device is arranged on the hollow part.
6. The loading device of claim 4, wherein: the sample loading device further comprises a cleaning mechanism, the cleaning mechanism is provided with a part for coating the sample feeding device, and the cleaning mechanism is provided with another part for communicating with the cleaning liquid inflow inlet.
7. The loading device of claim 1, wherein: the sample loading device further comprises a waste liquid collector, wherein a circulation hole is formed in the test tube carrier, and the waste liquid collector is provided with a waste liquid outflow port connected with the circulation hole.
8. The loading device of claim 1, wherein: the sample loading device further comprises a door, and the door can be arranged on the door frame in an opening and closing mode.
9. The loading device of claim 8, wherein: the sample loading device further comprises a third driving device, one part of the third driving device is connected with the first driving device, and the other part of the third driving device is connected with the door.
10. The loading device of claim 8, wherein: the sample loading device further comprises a reset mechanism, one part of the reset mechanism is arranged on the door frame, the other part of the reset mechanism is arranged on the door, and the door can be closed through the reset mechanism after being opened.
11. The loading device of claim 10, wherein: the sample loading device further comprises a magnetic suction mechanism, one part of the magnetic suction mechanism is arranged on the door frame, and the other part of the magnetic suction mechanism is arranged on the door.
12. Analytical sorting equipment, characterized by: the analytical sorting device comprises the sample loading device of any one of claims 1 to 11 and a flow chamber connected with the sample loading device, and further comprises a detection module, a control module and a sorting module, wherein a sample liquid is transferred from the sample loading device to the flow chamber, the detection module is used for detecting and acquiring at least one characteristic of biological particles in the sample liquid, the control module sorts and sorts the liquid drops according to the at least one characteristic of the biological particles in the liquid drops, and the sorting module places the liquid drops into a designated position by utilizing the sorting and sorting of the liquid drops.
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| CN202110652413.8A CN113358435A (en) | 2021-06-11 | 2021-06-11 | Sample loading device and analysis and sorting equipment comprising same |
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