CN107843542A - The specimen cup that a kind of cellanalyzer uses - Google Patents
The specimen cup that a kind of cellanalyzer uses Download PDFInfo
- Publication number
- CN107843542A CN107843542A CN201711210136.5A CN201711210136A CN107843542A CN 107843542 A CN107843542 A CN 107843542A CN 201711210136 A CN201711210136 A CN 201711210136A CN 107843542 A CN107843542 A CN 107843542A
- Authority
- CN
- China
- Prior art keywords
- rear chamber
- cup
- protecgulum
- inlet
- liquid outlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007788 liquid Substances 0.000 claims abstract description 42
- 238000004140 cleaning Methods 0.000 claims abstract description 13
- 208000002925 dental caries Diseases 0.000 claims abstract description 7
- 239000012530 fluid Substances 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 description 12
- 210000003660 reticulum Anatomy 0.000 description 11
- 239000012895 dilution Substances 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 210000001367 artery Anatomy 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1028—Sorting particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1029—Particle size
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses the specimen cup that a kind of cellanalyzer uses, the specimen cup includes two cavitys of cup and rear chamber, between connected by counting hole, cup electrode and rear chamber electrode are respectively equipped with cup and rear chamber, wherein:The rear chamber is formed after being connected by the rear chamber protecgulum with the first cavity with the rear chamber bonnet with the second cavity, dividing plate is provided between rear chamber protecgulum and rear chamber bonnet, there is the passage passed through for liquid on dividing plate, the bore of the feeder connection is less than the bore of its outlet, and the center line of passage overlaps with the center line of counting hole;The first inlet and the first liquid outlet are provided with rear chamber protecgulum, covered after rear chamber provided with the second inlet and the second liquid outlet, the first inlet and the second inlet, the first liquid outlet and the second liquid outlet are respectively through pipeline connection of the flexible pipe with the equipment of offer negative pressure and with providing cleaning fluid.Specimen cup of the present invention can effectively control influence of the turbulent flow resistance variations close counting hole area electrodes in rear chamber.
Description
Technical field
The present invention relates to a kind of specimen cup, and in particular to the specimen cup that a kind of cellanalyzer uses.
Background technology
The size of leucocyte can be classified according to its volume size within the specific limits in normal human blood, at present
Three classification blood cell analyzers (also referred to as " electrical impedance method " or " Kurt method ") carry out blood count using " orifice method "
And stereometry, its Cleaning Principle are that haemocyte will produce resistance variations when passing through aperture, realize hemocytometer accordingly
Several automations.
The critical piece of three classification blood cell analyzers is aperture plate, also referred to as sensor (Transducer).Aperture
Plate is inserted into the cell suspension of isotonic electrolyte solution dilution, and dilution is filled with the inside of aperture plate, and has an interior electrode, outside
There is an external electrode side.The conduction property of cell is lower than isotonic dilution, after electrode switches on power, positioned at aperture both sides
Electrode between produce stable electric current, dilution is flowed by aperture.The conductibility of cell is less than dilution, when cell pass through it is small
Kong Shi, resistance increase, causes voltage change in moment and a pulse signal occurs, be referred to as passing through arteries and veins in aperture induction zone
Punching.Voltage change caused by cell volume is bigger is bigger, caused higher by pulse amplitude.When electric current I and impedance Z are stable
, cell volume can be determined by the measurement to impulse magnitude, distinguish different types of cell, the number of recording impulse
The result of cell count can be obtained.
The sample cup structure that existing three classification cellanalyzer uses is as shown in figure 1, including two chambers of cup and rear chamber
Body, cup electrode and rear chamber electrode being respectively arranged with cup and rear chamber, the cup is connected with rear chamber by counting hole, after
A liquid outlet and an inlet are provided with room housing.As shown in Figure 1, the rear chamber of existing specimen cup is a cavity, is adopted
It is the liquid stream cleaning way of one-in-and-one-out.The applicant in experiments it is found that, the specimen cup of existing structure exist it is following not
Foot:
(1) because rear chamber is single cavity geometry, when (including particle (i.e. cell) and liquid) passes through counting hole in sample
When, the easily change in rear chamber resistance between electrode before and after bore region turbulization and then influence is counted, cause count results not
Accurately.
(2) there is corner in rear chamber, be not easy to clean up in the short time, it is possible to subsequent counter can be had an impact.
The content of the invention
The technical problem to be solved in the present invention is to provide the specimen cup that a kind of cellanalyzer uses, the specimen cup passes through
Dividing plate is set in rear chamber and structure of two liquid outlets simultaneously with soft pipe connection is set, effectively controls turbulent flow to close in rear chamber
The influence of resistance variations between counting hole area electrodes, improve and count accuracy rate.
The specimen cup that cellanalyzer of the present invention uses, including two cavitys of cup and rear chamber, in cup and
Cup electrode and rear chamber electrode are respectively arranged with rear chamber, the cup is connected with rear chamber by counting hole, wherein:
The rear chamber is formed after being connected by the rear chamber protecgulum with the first cavity with the rear chamber bonnet with the second cavity,
Dividing plate is provided between rear chamber protecgulum and rear chamber bonnet, there is the passage passed through for liquid, the mouth of the feeder connection on the dividing plate
Footpath is less than the bore of its outlet, and the center line of the passage overlaps with the center line of counting hole;
The first inlet and the first liquid outlet are respectively arranged with rear chamber protecgulum, is covered after rear chamber and is provided with second and enters
Liquid mouth and the second liquid outlet, the first described inlet and the second inlet are connected by flexible pipe and the road for the equipment for providing negative pressure
Logical, described the first liquid outlet and the second liquid outlet passes through flexible pipe and the pipeline connection for providing cleaning fluid.
The present invention is divided into two cavitys connected by passage, when logical negative pressure counts, turbulent flow by setting dividing plate, by rear chamber
The change of rear chamber first half (i.e. rear chamber is close to counting bore region) resistance between electrode can not be influenceed at once because of spacers block;Separately
On the one hand, two liquid outlets are set, and with flexible pipe by the path connected of they and the equipment for providing negative pressure, so as to manufacture liquid stream
Time difference, control when once counting completion, sample (including cell and liquid) is just back to rear chamber first half;Two means
The influence for achieving effective control turbulent flow to resistance variations close counting hole area electrodes in rear chamber is combined, improves and counts accurately
Rate.
In above-mentioned technical proposal, the passage on the dividing plate is preferably the loudspeaker gradually increased in porch to exit bore
Shape, it so can effectively prevent the first half (i.e. rear chamber close to count bore region) in rear chamber by the cell of counting hole
Diffusion.
In above-mentioned technical proposal, the profile of dividing plate can be arranged as required to, and such as can be square or other irregular shapes
Shape, but preferably by baffle design into the horn shape gradually increased from porch to exit external diameter, so can be further
Reduce the corner for being not easy to be flushed in rear chamber so that it can clean cleaner within the relatively short time in cleaning, so as to
Instrument can be shortened and count the cycle, improve detection efficiency.
In above-mentioned technical proposal, the first inlet and the first liquid outlet are preferably respectively arranged at opposed on rear chamber protecgulum
Position, and the second inlet and the second liquid outlet cover opposed position after being then preferably respectively arranged at rear chamber, so may be used
To be more beneficial for cleaning rear chamber.
Equally consider from the corner that is not easy to be flushed to is reduced in rear chamber, rear chamber electrode is preferably arranged at rear chamber protecgulum
On the inwall of upper first cavity, and it will be covered after rear chamber between the bottom surface of the second cavity and its side using cambered surface connection or inclined-plane
Connection.Each face of the first cavity is formed on the rear chamber protecgulum in connection it is also preferred that being connected using cambered surface connection or inclined-plane.
In above-mentioned technical proposal, the rear chamber protecgulum and rear chamber bonnet are usually to be detachably connected, usually using screw
Realize the connection of rear chamber protecgulum, dividing plate and rear chamber bonnet three.
Compared with prior art, the method have the characteristics that:
1st, by setting dividing plate, by rear chamber be divided into two by passage connect cavitys, when logical negative pressure counts, turbulent flow because every
Plate stops and can not influence the change of rear chamber first half (i.e. rear chamber is close to counting bore region) resistance between electrode at once;The opposing party
Face, sets two liquid outlets, and with flexible pipe by the path connected of they and the equipment for providing negative pressure, so as to manufacture the time of liquid stream
Difference, control when once counting completion, sample (including cell and liquid) is just back to rear chamber first half;Two means are mutually tied
The influence for achieving effective control turbulent flow to resistance variations close counting hole area electrodes in rear chamber is closed, improves and counts accuracy rate.
2nd, using the rinse mode to go with each other all the time, effectively cleaning performance of the lifting to rear chamber.
Brief description of the drawings
Fig. 1 is the structural representation of the specimen cup that cellanalyzer uses in the prior art;
Fig. 2 is a kind of structural representation of embodiment of specimen cup that cellanalyzer of the present invention uses;
Fig. 3 is the installation diagram for each part that rear chamber is formed in Fig. 2 illustrated embodiments;
Fig. 4 is the simple schematic diagram of operation principle when being counted using the specimen cup of Fig. 2 illustrated embodiments;
Fig. 5 is the simple schematic diagram of operation principle in cleaning using the specimen cup of Fig. 2 illustrated embodiments.
In figure marked as:
1 cup;2 cup electrodes;3 rear chambers;4 rear chamber electrodes;5 first liquid outlets;6 first inlets;7 second liquid outlets;8
Second liquid outlet;9 rear chamber protecgulums;10 rear chamber bonnets;11 screws;12 dividing plates;13 flexible pipes.
Embodiment
With reference to specific embodiment, the present invention is described in further detail, to more fully understand present disclosure, but
The present invention is not limited to following examples.
As Figure 2-3, the specimen cup that cellanalyzer of the present invention uses, including cup 1 and rear chamber 3 two
Cavity, cup electrode 2 being provided with cup 1, the cup 1 is connected with rear chamber 3 by counting hole, wherein:
The rear chamber 3 can in process by the rear chamber protecgulum 9 with the first cavity and the rear chamber bonnet 10 with the second cavity
Formed, connected on the rear chamber bonnet 10 between the bottom surface and its side of second cavity for inclined-plane, the rear chamber after dismantling connection
Each face that the first cavity is formed on protecgulum 9 also connects in connection for inclined-plane;On rear chamber protecgulum 9 on the inwall of first cavity
Rear chamber electrode 4 is provided with, an annular slot is further opened with the rear chamber protecgulum 9, the annular slot is located at the of rear chamber protecgulum 9
The joint of one cavity and the second cavity of rear chamber bonnet 10;
Dividing plate 12 is provided between rear chamber protecgulum 9 and rear chamber bonnet 10, there is the passage passed through for liquid on dividing plate 12,
Rear chamber 3 is divided into two cavitys connected by passage by the setting of the dividing plate 12;The profile of the dividing plate 12 is in from porch to going out
The horn shape that external diameter gradually increases at mouthful, and exit has towards the snap ring extended with the direction of the axis perpendicular of dividing plate 12,
The shape of the snap ring is corresponding to the shape of annular slot on rear chamber protecgulum 9 so that after dividing plate 12 can be placed in by snap ring thereon
On room protecgulum 9;
The bore of passage its entrance on the dividing plate 12 is less than the bore of its outlet, and is in porch to exit bore
The horn shape gradually increased, and the center line of the passage overlaps with the center line of counting hole;
The first inlet 6 and the first liquid outlet 5 are respectively arranged with rear chamber protecgulum 9, is provided with rear chamber bonnet 10
Second inlet 8 and the second liquid outlet 7, the first described inlet 6, the first liquid outlet 5, the second inlet 8 and second go out liquid
Mouth 7 connects with rear chamber 3;Described the first inlet 6 and the second inlet 8 passes through flexible pipe 13 and the road for the equipment for providing negative pressure
Road connects, and the first described liquid outlet 5 and the second liquid outlet 7 pass through flexible pipe 13 and the pipeline connection for providing cleaning fluid.
In present embodiment, connected between the rear chamber protecgulum 9, dividing plate 12 and the three of rear chamber bonnet 10 using the connection of screw 11
Connect.
Specimen cup of the present invention is divided into two cavitys connected by passage by setting dividing plate 12, by rear chamber 3, logical negative
When pressure counts, turbulent flow can not influence the first half of rear chamber 3 (i.e. rear chamber 3 is close to counting bore region) electricity at once because dividing plate 12 stops
The change of electrode resistance;On the other hand, two liquid outlets are set, and with flexible pipe 13 by the road of they and the equipment for providing negative pressure
Connection, so as to manufacture the time difference of liquid stream, control when once counting completion, after sample (including cell and liquid) is just back to
The first half of room 3, as shown in Figure 4;Two means, which are combined, achieves effective control turbulent flow to electric close to bore region is counted in rear chamber 3
The influence of electrode resistance change, improves and counts accuracy rate.In addition, using the rinse mode (as shown in Figure 5) to go with each other all the time, cleaning
When the first half of rear chamber 3 and latter half have inlet and liquid outlet, beneficial to cleaning rear chamber 3 in each corner, effectively carry
Rise the cleaning performance to rear chamber 3.
Claims (6)
1. the specimen cup that a kind of cellanalyzer uses, including (3) two cavitys of cup (1) and rear chamber, cup (1) and after
Cup electrode (2) and rear chamber electrode (4) are respectively arranged with room (3), the cup (1) is connected with rear chamber (3) by counting hole,
It is characterized in that:
After the rear chamber (3) is by the rear chamber protecgulum (9) with the first cavity and the connection of the rear chamber bonnet (10) with the second cavity
Formed, dividing plate (12) is provided between rear chamber protecgulum (9) and rear chamber bonnet (10), had on the dividing plate (12) and pass through for liquid
Passage, the bore of the feeder connection is less than the bore of its outlet, and the center line of the passage overlaps with the center line of counting hole;
The first inlet (6) and the first liquid outlet (5) are respectively arranged with rear chamber protecgulum (9), is set on rear chamber bonnet (10)
The second inlet (8) and the second liquid outlet (7) are equipped with, described the first inlet (6) and the second inlet (8) pass through flexible pipe
(13) with the path connected for the equipment for providing negative pressure, described the first liquid outlet (5) and the second liquid outlet (7) pass through flexible pipe (13)
Pipeline connection with providing cleaning fluid.
2. the specimen cup that cellanalyzer according to claim 1 uses, it is characterised in that:On the dividing plate (12)
Passage is in the horn shape that porch to exit bore gradually increases.
3. the specimen cup that cellanalyzer according to claim 1 uses, it is characterised in that:Described dividing plate (12) is in
The horn shape that porch to exit external diameter gradually increases.
4. the specimen cup that the cellanalyzer according to any one of claim 1-3 uses, it is characterised in that:Described
Rear chamber electrode (4) is arranged at the inwall of the first cavity on rear chamber protecgulum (9).
5. the specimen cup that the cellanalyzer according to any one of claim 1-3 uses, it is characterised in that:After described
Room protecgulum (9) and rear chamber bonnet (10) are to be detachably connected.
6. the specimen cup that the cellanalyzer according to any one of claim 1-3 uses, it is characterised in that:After described
Connected on room bonnet (10) between the bottom surface and its side of the second cavity for cambered surface connection or inclined-plane.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711210136.5A CN107843542B (en) | 2017-11-27 | 2017-11-27 | Sample cup for blood cell analyzer |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711210136.5A CN107843542B (en) | 2017-11-27 | 2017-11-27 | Sample cup for blood cell analyzer |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107843542A true CN107843542A (en) | 2018-03-27 |
CN107843542B CN107843542B (en) | 2023-05-30 |
Family
ID=61680452
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201711210136.5A Active CN107843542B (en) | 2017-11-27 | 2017-11-27 | Sample cup for blood cell analyzer |
Country Status (1)
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CN (1) | CN107843542B (en) |
Citations (13)
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CN101246113A (en) * | 2007-02-14 | 2008-08-20 | 深圳迈瑞生物医疗电子股份有限公司 | Monitoring method for hemoglobin test result |
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CN104458540A (en) * | 2014-12-04 | 2015-03-25 | 南昌百特生物高新技术股份有限公司 | Flow chamber of blood analyzer |
CN206235543U (en) * | 2016-08-24 | 2017-06-09 | 桂林优利特医疗电子有限公司 | Particle analyzer |
CN207457028U (en) * | 2017-11-27 | 2018-06-05 | 桂林优利特医疗电子有限公司 | The specimen cup that a kind of cellanalyzer uses |
-
2017
- 2017-11-27 CN CN201711210136.5A patent/CN107843542B/en active Active
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CN101246113A (en) * | 2007-02-14 | 2008-08-20 | 深圳迈瑞生物医疗电子股份有限公司 | Monitoring method for hemoglobin test result |
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CN103785491A (en) * | 2014-01-28 | 2014-05-14 | 桂林优利特医疗电子有限公司 | Disposable specimen cup, blood analyzer and use method |
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