CN102564918A - Particle counting device and blood cell analysis device - Google Patents
Particle counting device and blood cell analysis device Download PDFInfo
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- CN102564918A CN102564918A CN2010106197053A CN201010619705A CN102564918A CN 102564918 A CN102564918 A CN 102564918A CN 2010106197053 A CN2010106197053 A CN 2010106197053A CN 201010619705 A CN201010619705 A CN 201010619705A CN 102564918 A CN102564918 A CN 102564918A
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Abstract
The invention discloses a particle counting device. The particle counting device comprises a front pool part, a rear pool part, a counting component arranged between the front pool part and the rear pool part, wherein the rear pool part comprises a rear pool shell, a rear sheath inlet, a capturing pipe, a waste liquid outlet, a rear pool cleaning outlet and a rear pool; the rear pool is formed by an internal cavity of the rear pool shell; the rear sheath inlet is arranged on the rear pool shell and is communicated with the rear pool; one end of the capturing pipe, which is close to the counting component, is provided with a capturing pipe inlet, and the other end of the capturing pipe, which is far away from a technical component, is communicated with the waste liquid outlet; and the rear pool cleaning outlet is arranged at the middle part of the capturing pipe, and is respectively communicated with the rear pool and the waste liquid outlet. The invention also discloses a blood cell analysis device adopting the particle counting device. The particle counting device and blood cell analysis device disclosed by the invention have the advantages of simple structure and high reagent utilization ratio.
Description
Technical field
The present invention relates to the particle analysis field, particularly a kind of particle counting device and blood cell analytical equipment that is applicable to blood analyser.
Background technology
In the medical system, often need analyze to obtain various parameters, the scatter diagram of for example various particles or specified particle, and statistics particle (for example cell) quantity in modern times particle in the body fluid especially blood.The particle analyzer that is used for analytic statistics blood cell or other body fluid cell is also referred to as cytoanalyze usually.
Sheath flow impedance technology is a kind of counting technology commonly used in the blood analyser.Seeing also Fig. 1, is the schematic cross-section that prior art adopts the counter of sheath flow impedance technology.Counter comprises that forebay 3, after-bay 10, forebay housing 1, protheca inlet 2, specimen needle 4, specimen needle holder 5, forebay clean inlet 6, after-bay housing 13, back sheath inlet 9, catch pipe 11, catch tube inlet 8, waste liquid outlet 12 and after-bay washing outlet 14.。Specimen needle 4 is exposed the outer end of counter and is comprised sample liquid inlet 16, and specimen needle comprises specimen needle outlet 33 at the inner other end of counter.Counter also comprises the jewel sheet 15 that is provided with between forebay 3 and the after-bay 10, on jewel sheet 15, be provided with specimen needle export 33 over against counting aperture 7.
When containing the particle sample and count, protheca flows into from protheca inlet 2, the inflow from the sheath inlet 9 of back of back sheath.The sample liquid that includes molecule flows into from specimen needle inlet 16; Sample liquid flows out the back from specimen needle outlet 33 and under the parcel of protheca, passes through to count aperture 7, and sample liquid and protheca flow out from waste liquid outlet 12 through catching in the tube inlet 8 entering seizure pipes under the parcel of back sheath at last afterwards.Because reagent is the good conductor of electricity; And particle is the poor conductor of electricity; When particle through counting during aperture 7, the resistance of 15 both sides, jewel hole will be obviously greater than no particle when counting aperture 7, at this time detect the relevant information that resistance between forebay 3 and the after-bay 10 just can obtain particle.The measuring principle of Here it is sheath flow impedance counting.
For guaranteeing the accuracy of counting, there are not bubble and other particle in the pond before and after requiring during measurement, so need clean to the pond, front and back.In the counter as shown in Figure 1, when cleaning in the forebay, cleaning fluid cleans the inlet 6 from the forebay and flows into, and from protheca inlet 2, flows out; Sheath inlet 9 flows into cleaning fluid from the back when after-bay is cleaned, and flows out from after-bay washing outlet 14.In addition, above-mentioned counter also comprises specimen needle 4 and catches pipe 11 these two pipelines.Therefore, common counter needs 6 pipelines to realize various functions in the prior art, makes that its liquid line structure is comparatively complicated.
Summary of the invention
In order to solve prior art particle counting device liquid line structure complicated problems, be necessary to provide comparatively simple particle counting device of a kind of structure and blood cell analytical approach.
The present invention's one preferred implementation provide a kind of particle counting device to comprise forebay portion, after-bay portion and be arranged at forebay portion and after-bay portion between the counting assembly; Said after-bay portion comprises after-bay housing, back sheath inlet, catches pipe, waste liquid outlet, after-bay washing outlet and after-bay; After-bay enclosure interior cavity forms after-bay; Said back sheath inlet is arranged on the after-bay housing and with after-bay and communicates; The contiguous counting of said seizure pipe assembly one end has catches tube inlet and communicates with waste liquid outlet away from the other end of technology component, and the after-bay washing outlet is arranged on to catch in the middle part of the pipe and with said after-bay and said waste liquid outlet and is communicated with respectively.
In the above-mentioned particle counting device, said counting assembly is a schistose texture, and the counting aperture is set on it, and said forebay and after-bay are communicated with through said counting aperture.
In the above-mentioned particle counting device; Said forebay portion comprises that forebay housing, protheca inlet, specimen needle, specimen needle holder, forebay clean inlet and forebay; Specimen needle exposes that a housing outer end in forebay comprises the specimen needle inlet and the other end in the portion of forebay comprises the specimen needle outlet; Said forebay is that forebay enclosure interior cavity forms, and protheca inlet and forebay are cleaned inlet and be separately positioned on the housing of forebay, and all communicate with the forebay.
In the above-mentioned particle counting device, said forebay portion and after-bay portion are the cylindrical structure with internal chamber, its axis and surface level coplane, and with the axis of axis, the specimen needle of counting aperture, catch the axis conllinear of pipe.
The present invention's one preferred implementation provides a kind of particle counting device; Comprise forebay portion, after-bay portion and be arranged on the two counting assembly between straight; The after-bay washing outlet that said after-bay portion comprises the back sheath inlet that is used for when counting, providing back sheath liquid to get into the passage of after-bay and the passage that cleaning fluid gets into after-bay is provided when cleaning, be used to cleaning fluid to be provided and to get into the passage of waste liquid outlet, be used to comprise waste liquid outlet that liquid such as the cleaning fluid that flows to the sample liquid of catching pipe, flow into from the after-bay washing outlet, sheath fluid discharge, catch pipe and have the after-bay housing of cavity; Said after-bay enclosure interior has cavity and forms after-bay; Said back sheath inlet is arranged on the after-bay housing; Said seizure Guan Yiduan has the seizure tube inlet, and the other end is communicated with waste liquid outlet, the setting of the contiguous counting of said seizure tube inlet assembly.
In the above-mentioned particle counting device; Said forebay portion comprises the protheca inlet that is used for when counting, providing the passage that protheca liquid enters into the forebay and the passage that cleaning fluid flows out the forebay is provided when cleaning, be used for when cleaning, providing the forebay of the passage that cleaning liquid gets into the forebay to clean inlet, specimen needle and forebay housing; Said forebay enclosure interior has cavity and forms the forebay; The end that said specimen needle is positioned at outside the portion of forebay comprises the specimen needle inlet; An end that is positioned at forebay portion comprises the specimen needle outlet, and said protheca inlet and forebay are cleaned to enter the mouth to be arranged on the housing of forebay and also be communicated with the forebay respectively.
In the above-mentioned particle counting device; Several of said countings have the counting aperture; Said forebay portion and after-bay portion are the cylindrical structure with internal chamber, its axis and surface level coplane, and with the axis of the axis of said counting aperture, said specimen needle and the axis conllinear of said seizure pipe.
In the above-mentioned particle counting device; During counting; Protheca liquid flows into from the protheca inlet, and back sheath liquid flows into from the sheath inlet of back, and the sample liquid that includes molecule flows into from the specimen needle inlet; Sample liquid passes through the counting aperture from specimen needle outlet outflow back under the parcel of protheca liquid, sample liquid and protheca get into the seizure pipe through the seizure tube inlet and from waste liquid outlet, flow out at last under the parcel of back sheath liquid afterwards; During cleaning, the cleaning of said forebay is cleaned from the forebay by cleaning fluid that inlet flows into the forebay and is flowed out from the protheca inlet and carries out, and the sheath inlet flows into and flows out via after-bay washing outlet, waste liquid outlet and carries out from the back by cleaning fluid in the cleaning of said after-bay.
Blood cell analytical equipment of the present invention comprises above-mentioned arbitrary described particle counting device.
Compared to prior art; Do not have special after-bay washing outlet in the particle counting device of the present invention; But in catching pipe, increased a passage (being the after-bay washing outlet) newly; And be communicated with after-bay and waste liquid outlet, thus cleaning fluid can get into the seizure pipe from after-bay washing outlet and seizure tube inlet respectively when after-bay is cleaned, and then flow out from waste liquid outlet.Therefore, particle counting device of the present invention has been cancelled independent after-bay washing outlet, adopts waste liquid outlet to discharge cleaning fluid, and pipeline structure is comparatively simple.In addition, employed cleaning fluid can continue to clean waste liquid outlet and follow-up pipeline when cleaning after-bay, has also improved the utilization factor of cleaning fluid.Same, the counter tube line structure of blood cell analytical equipment of the present invention is comparatively simple.
Description of drawings
Fig. 1 is the partial cross section synoptic diagram of prior art particle counting device.
Fig. 2 is the partial cross section synoptic diagram of particle counting device of the present invention.
Embodiment
In conjunction with the accompanying drawings the present invention is further explained below by the specific embodiment.
Seeing also Fig. 2, is the partial cross section synoptic diagram of corpuscular counter one preferred embodiments of the present invention.This counter comprises forebay portion (not indicating), after-bay portion (not indicating) and is arranged on the counting assembly 32 between forebay and the after-bay.Portion gets into and passes counting assembly 32 backs and discharges from after-bay portion the sample liquid that contains fine particle from the forebay, thereby accomplishes particle counting.
Said forebay portion comprises that forebay housing 17, protheca inlet 18, specimen needle 20, specimen needle holder 19, forebay clean inlet 23 and forebay 22.The end that specimen needle is exposed outside the portion of forebay comprises specimen needle inlet 21, and the other end in the portion of forebay comprises specimen needle outlet 34.The relative position that said specimen needle holder 19 is used between specimen needle and the forebay housing is fixed.Said after-bay portion comprises after-bay housing 30, back sheath inlet 26, catches pipe 29, waste liquid outlet 28, after-bay washing outlet and after-bay 31.Catch pipe 29 contiguous counting assemblies 32 1 ends and have the tube inlet 25 of seizure, communicate with waste liquid outlet 28 away from the other end of technology component 32.
Counting assembly 32 is arranged between forebay portion and the after-bay portion, and it has counting aperture 24.Counting aperture 24 and the specimen needle outlet 34 of forebay portion, the seizure tube inlet 25 corresponding coaxial settings of after-bay.Forebay 22 and after-bay 31 through and only through said counting aperture 24 UNICOMs.
Forebay portion and after-bay portion are the cylindrical structure with internal chamber, its axis and surface level coplane, and with the axis of axis, the specimen needle 20 of counting aperture 24, catch the axis conllinear of pipe 29.Housing 17 internal cavities in forebay form forebay 22.After-bay housing 30 internal cavities form after-bay 31.Protheca inlet 23 cleans inlet 18 with the forebay and is separately positioned on the forebay housing 17, and all communicates with forebay 22.Back sheath inlet 26 is arranged on the after-bay housing 30 and with after-bay 31 and communicates.After-bay washing outlet 27 is arranged on catches pipe 29 middle parts and is communicated with after-bay 31 and waste liquid outlet 28.As shown in Figure 2.Catch pipe 29 and be used to collect sample liquid and sheath flow liquid body.Sample liquid flows out and counts the back through counting assembly 32 from specimen needle outlet 34 and gets into from catching tube inlet 25.Protheca inlet 18 is used for when counting, providing the passage that protheca liquid enters into forebay 22 and when cleaning, provides cleaning fluid to flow out the passage of forebay 22.The forebay is cleaned inlet 23 and is used for when cleaning, providing cleaning liquid to get into the passage of forebay 22.Back sheath inlet 26 is used for when counting, providing back sheath liquid to get into the passage of after-bay 31 and when cleaning, provide cleaning fluid to get into the passage of after-bay 31.After-bay washing outlet 27 is used to provide cleaning fluid and the passage that gets into waste liquid outlet 28.Waste liquid outlet 28 is used to comprise that the cleaning fluid that flows to the sample liquid of catching pipe 29, flows into from after-bay washing outlet 27, the liquid such as sheath fluid in the after-bay 31 discharge.
During counting, protheca liquid flows into from protheca inlet 18, and back sheath liquid flows into from back sheath inlet 26.The sample liquid that includes molecule flows into from specimen needle inlet 21; Sample liquid passes through counting aperture 24 under the parcel of protheca liquid after specimen needle outlet 34 is flowed out, sample liquid and protheca are caught pipe 29 and outflow from waste liquid outlet 12 at last through 25 entering of seizure tube inlet under the parcel of back sheath liquid afterwards.
During cleaning; The cleaning of forebay 22 is cleaned inlet 23 inflow forebays 22 by cleaning fluid and is flowed out from protheca inlet 23 and carries out from the forebay, sheath inlet 25 flows into and flows out via after-bay washing outlet 27, waste liquid outlet 28 and carries out from the back by cleaning fluid in the cleaning of after-bay 31.
In this embodiment, specimen needle 20 and seizure pipe 29 are separately positioned on the central axis of forebay 22 and after-bay 31, and protheca inlet 18 and forebay cleaning inlet 23 are with respect to the central shaft setting of forebay portion.Same, back sheath inlet 26 is provided with respect to after-bay portion central shaft with after-bay washing outlet 27.Counting assembly 32 is the jewel sheets with jewel hole, and jewel hole and specimen needle outlet and seizure tube inlet are over against coaxial setting.
Other embodiments of the present invention and above-mentioned embodiment have roughly the same structure, and difference is: on the after-bay housing of after-bay portion a plurality of after-bay washing outlet can be set, these a plurality of after-bay washing outlet are communicated with after-bay and the waste liquid outlet of catching Guan Yiduan respectively.Blood cell analytical equipment of the present invention comprises particle analysing device of the present invention.
Compared to prior art; Particle counting device of the present invention and blood cell analytical equipment do not have special after-bay washing outlet; But in catching pipe 29, increased a passage (being after-bay washing outlet 27) newly; And be communicated with after-bay 31 and waste liquid outlet 28, thus cleaning fluid can be caught pipe 29 from after-bay washing outlet 27 and 25 entering of seizure tube inlet respectively when after-bay is cleaned, and then from waste liquid outlet 28 outflows.Therefore, particle counting device of the present invention has been cancelled independent after-bay washing outlet, adopts waste liquid outlet 28 to discharge cleaning fluid, and pipeline structure is comparatively simple.In addition, employed cleaning fluid can continue to clean waste liquid outlet 12 and follow-up pipeline when cleaning after-bay, has also improved the utilization factor of cleaning fluid.
Above content is to combine concrete embodiment to the further explain that the present invention did, and can not assert that practical implementation of the present invention is confined to these explanations.For the those of ordinary skill of technical field under the present invention, under the prerequisite that does not break away from the present invention's design, can also make some simple deduction or replace, all should be regarded as belonging to protection scope of the present invention.
Claims (9)
1. particle counting device; It is characterized in that: comprise forebay portion, after-bay portion and be arranged at forebay portion and after-bay portion between the counting assembly; Said after-bay portion comprises after-bay housing, back sheath inlet, catches pipe, waste liquid outlet, after-bay washing outlet and after-bay; Said after-bay is that after-bay enclosure interior cavity forms; Sheath enters the mouth to be arranged on the after-bay housing and with after-bay and communicates after said, and the contiguous counting of said seizure pipe assembly one end has the seizure tube inlet and said seizure pipe communicates with waste liquid outlet away from the other end of counting assembly, and said after-bay washing outlet is arranged on seizure pipe middle part and is communicated with respectively with said after-bay and said waste liquid outlet.
2. particle counting device according to claim 1 is characterized in that: said counting assembly is a schistose texture, and the counting aperture is set on it, and said forebay and after-bay are communicated with through said counting aperture.
3. particle counting device according to claim 2; It is characterized in that: said forebay portion comprises that forebay housing, protheca inlet, specimen needle, specimen needle holder, forebay clean inlet and forebay; Specimen needle exposes that a housing outer end in forebay comprises the specimen needle inlet and the other end in the portion of forebay comprises the specimen needle outlet; Said forebay is that forebay enclosure interior cavity forms, and protheca inlet and forebay are cleaned inlet and be separately positioned on the housing of forebay, and all communicate with the forebay.
4. particle counting device according to claim 3; It is characterized in that: said forebay portion and after-bay portion are the cylindrical structure with internal chamber; Its axis and surface level coplane, and with the axis of axis, the specimen needle of counting aperture, catch the axis conllinear of pipe.
5. particle counting device; It is characterized in that: comprise forebay portion, after-bay portion and be arranged on the counting assembly between the two; The after-bay washing outlet that said after-bay portion comprises the back sheath inlet that is used for when counting, providing back sheath liquid to get into the passage of after-bay and the passage that cleaning fluid gets into after-bay is provided when cleaning, be used to cleaning fluid to be provided and to get into the passage of waste liquid outlet, be used to comprise waste liquid outlet that liquid such as the cleaning fluid that flows to the sample liquid of catching pipe, flow into from the after-bay washing outlet, sheath fluid discharge, catch pipe and have the after-bay housing of cavity; Said after-bay enclosure interior has cavity and forms after-bay; Said back sheath inlet is arranged on the after-bay housing; Said seizure Guan Yiduan has the seizure tube inlet, and the other end is communicated with waste liquid outlet, the setting of the contiguous counting of said seizure tube inlet assembly.
6. particle counting device according to claim 5; It is characterized in that: said forebay portion comprises the protheca inlet that is used for when counting, providing the passage that protheca liquid enters into the forebay and the passage that cleaning fluid flows out the forebay is provided when cleaning, be used for when cleaning, providing the forebay of the passage that cleaning liquid gets into the forebay to clean inlet, specimen needle and forebay housing; Said forebay enclosure interior has cavity and forms the forebay; The end that said specimen needle is positioned at outside the portion of forebay comprises the specimen needle inlet; An end that is positioned at forebay portion comprises the specimen needle outlet, and said protheca inlet and forebay are cleaned to enter the mouth to be arranged on the housing of forebay and also be communicated with the forebay respectively.
7. particle counting device according to claim 6; It is characterized in that: said counting assembly has the counting aperture; Said forebay portion and after-bay portion are the cylindrical structure with internal chamber; Its axis and surface level coplane, and with the axis of the axis of said counting aperture, said specimen needle and the axis conllinear of said seizure pipe.
8. particle counting device according to claim 7; It is characterized in that: during counting; Protheca liquid flows into from the protheca inlet, and back sheath liquid flows into from the sheath inlet of back, and the sample liquid that includes molecule flows into from the specimen needle inlet; Sample liquid passes through the counting aperture from specimen needle outlet outflow back under the parcel of protheca liquid, sample liquid and protheca get into the seizure pipe through the seizure tube inlet and from waste liquid outlet, flow out at last under the parcel of back sheath liquid afterwards; During cleaning, the cleaning of said forebay is cleaned from the forebay by cleaning fluid that inlet flows into the forebay and is flowed out from the protheca inlet and carries out, and the sheath inlet flows into and flows out via after-bay washing outlet, waste liquid outlet and carries out from the back by cleaning fluid in the cleaning of said after-bay.
9. a blood cell analytical equipment is characterized in that: comprise arbitrary described particle counting device according to claim 1-8.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010619705.3A CN102564918B (en) | 2010-12-31 | 2010-12-31 | Particle counting device and blood cell analysis device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010619705.3A CN102564918B (en) | 2010-12-31 | 2010-12-31 | Particle counting device and blood cell analysis device |
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| CN102564918A true CN102564918A (en) | 2012-07-11 |
| CN102564918B CN102564918B (en) | 2015-12-02 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103785491A (en) * | 2014-01-28 | 2014-05-14 | 桂林优利特医疗电子有限公司 | Disposable specimen cup, blood analyzer and use method |
| CN106404637A (en) * | 2016-10-08 | 2017-02-15 | 重庆医科大学附属永川医院 | Hemocyte analyzer for medical detection |
| CN107843542A (en) * | 2017-11-27 | 2018-03-27 | 桂林优利特医疗电子有限公司 | The specimen cup that a kind of cellanalyzer uses |
| CN112444619A (en) * | 2019-08-30 | 2021-03-05 | 深圳迈瑞生物医疗电子股份有限公司 | Impedance method counting device and blood cell analyzer |
| CN114062228A (en) * | 2020-08-07 | 2022-02-18 | 北京鑫骥金诺医疗器械有限公司 | Bacteria counting device with sheath flow impedance sensor and method thereof |
| CN114632758A (en) * | 2020-12-16 | 2022-06-17 | 深圳市帝迈生物技术有限公司 | Cleaning device, liquid path cleaning method and sample analyzer |
| CN115266539A (en) * | 2021-04-30 | 2022-11-01 | 深圳市帝迈生物技术有限公司 | Sheath flow impedance counting device and detection method |
| CN115389399A (en) * | 2021-05-24 | 2022-11-25 | 深圳市帝迈生物技术有限公司 | Sheath flow impedance counting device and detection method |
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| CN103785491A (en) * | 2014-01-28 | 2014-05-14 | 桂林优利特医疗电子有限公司 | Disposable specimen cup, blood analyzer and use method |
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| CN106404637A (en) * | 2016-10-08 | 2017-02-15 | 重庆医科大学附属永川医院 | Hemocyte analyzer for medical detection |
| CN106404637B (en) * | 2016-10-08 | 2018-11-13 | 重庆医科大学附属永川医院 | A kind of medicine detection cellanalyzer |
| CN107843542A (en) * | 2017-11-27 | 2018-03-27 | 桂林优利特医疗电子有限公司 | The specimen cup that a kind of cellanalyzer uses |
| CN112444619A (en) * | 2019-08-30 | 2021-03-05 | 深圳迈瑞生物医疗电子股份有限公司 | Impedance method counting device and blood cell analyzer |
| CN112444619B (en) * | 2019-08-30 | 2023-12-19 | 深圳迈瑞动物医疗科技股份有限公司 | Impedance method counting device and blood cell analyzer |
| CN114062228A (en) * | 2020-08-07 | 2022-02-18 | 北京鑫骥金诺医疗器械有限公司 | Bacteria counting device with sheath flow impedance sensor and method thereof |
| CN114632758A (en) * | 2020-12-16 | 2022-06-17 | 深圳市帝迈生物技术有限公司 | Cleaning device, liquid path cleaning method and sample analyzer |
| CN114632758B (en) * | 2020-12-16 | 2024-04-09 | 深圳市帝迈生物技术有限公司 | Cleaning device, liquid path cleaning method and sample analyzer |
| CN115266539A (en) * | 2021-04-30 | 2022-11-01 | 深圳市帝迈生物技术有限公司 | Sheath flow impedance counting device and detection method |
| CN115389399A (en) * | 2021-05-24 | 2022-11-25 | 深圳市帝迈生物技术有限公司 | Sheath flow impedance counting device and detection method |
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| CN102564918B (en) | 2015-12-02 |
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Application publication date: 20120711 Assignee: Shenzhen Mindray Animal Medical Technology Co.,Ltd. Assignor: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd. Contract record no.: X2022440020009 Denomination of invention: Particle counting device and blood cell analysis device Granted publication date: 20151202 License type: Common License Record date: 20220804 |