CN103575636A - Sheath flow device of hematology analyzer - Google Patents
Sheath flow device of hematology analyzer Download PDFInfo
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- CN103575636A CN103575636A CN201310560181.9A CN201310560181A CN103575636A CN 103575636 A CN103575636 A CN 103575636A CN 201310560181 A CN201310560181 A CN 201310560181A CN 103575636 A CN103575636 A CN 103575636A
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Abstract
The invention discloses a sheath flow device of a hematology analyzer. The sheath flow device comprises a sheath flow device seat, a sheath flow chamber and a sheath flow device cover, wherein the sheath flow chamber and the sheath flow device cover are arranged at two ends of the sheath flow device seat; a sample injection needle is arranged in an inner cavity of the sheath flow device seat through a needle seat; the sheath flow chamber comprises a sheath flow chamber body which is of a cube shape; a flow-in cavity, a flow-out cavity and a test cavity communicating the flow-in cavity with the flow-out cavity are formed in the sheath flow chamber body; the test cavity is 60-80 microns high, and the aperture of the test cavity is 50-100 microns; the geometric center of the test cavity is superposed with that of the sheath flow chamber body; the flow-in cavity and the flow-out cavity are symmetric to each other about a horizontal line which penetrates through the geometric center of the test cavity and serves as a symmetric shaft; the center axis of the flow-in cavity, the center axis of the flow-out cavity and the longitudinal center axis of the test cavity are superposed with the longitudinal center axis of the sheath flow chamber body. The sheath flow device disclosed by the invention can simultaneously acquire laser scattering test data and impedance test data of a single cell while being linked with a system.
Description
Technical field
The present invention relates to cellanalyzer, be specifically related to a kind of cellanalyzer sheath flow device.
Background technology
The size of normal human's blood middle leukocytes can be classified according to its volume size within the specific limits, and current three classification blood cell analyzers all adopt " orifice method " (also claiming " electrical impedance method " or " Ku Ertefa ") to classify to it.But, similar acidophil, basocyte and the monocyte of leucocyte volume size all be distinguished, need to use five classification cellanalyzers.
In five classification cellanalyzers, in order to analyze the classification of each cell, must be by cell arrangement neatly one by one by laser detection district, accept Ear Mucosa Treated by He Ne Laser Irradiation, the method realizing is " Flow Cytometry " (also claiming " sheath stream technology "), in sheath stream chamber, by sheath fluid, " blood sample this " carries out " fluid-focus " to detecting, and makes sample to be tested form the liquid stream that a branch of diameter is about cell size, makes that cell is regular one by one to be detected by laser irradiation area.
Mostly the sheath flow device that cellanalyzer is used is at present to only have a sheath stream chamber, sheath stream chamber has fluid focus portion, one side of fluid focus portion has sample entrance and two sheath fluid entrances that arrange with sample entrance center axle rotational symmetry, and the opposite side of fluid focus portion has a sheath flow export.But when existing sheath flow device and system coupling, can only obtain the laser light scattering test data of individual cells, and can not obtain the testing impedance data of individual cells.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of cellanalyzer sheath flow device, can directly on the sheath stream chamber component of sheath flow device, add constant current source, make it when with system coupling, can obtain laser light scattering test data and the testing impedance data of individual cells simultaneously.
A kind of cellanalyzer sheath flow device, comprise sheath flow device seat, be installed on respectively sheath stream chamber and the sheath flow device lid at sheath flow device seat two ends, sample adds pin by needle stand, to be installed in the inner chamber of sheath flow device seat, on described needle stand, be also provided with two side pipes that add pin central shaft rotational symmetry to arrange with sample, wherein:
Described sheath stream chamber comprises the sheath stream chamber body of the side's of being shape, on the body of sheath stream chamber, be provided with an inflow chamber, an exhaust chamber, and the test chamber that is communicated with described inflow chamber and exhaust chamber, described inflow chamber and exhaust chamber are all taper type, described test chamber is square or is cylindrical, the height of described test chamber is 60～80 μ m, and its bore is 50～100 μ m; The geometric center of described test chamber overlaps with the geometric center of sheath stream chamber body, described inflow chamber and exhaust chamber be take and be symmetrical structure through the horizontal line of the geometric center of test chamber as axis of symmetry, and the central axis of described inflow chamber, longitudinal center's axis that longitudinal center's axis of the central axis of exhaust chamber and test chamber all flows chamber body with sheath overlap.
In technique scheme,
The height of described test chamber is preferably 70 μ m, and its bore is preferably 60 μ m.
The angle of the extended line of two buses of described inflow chamber or exhaust chamber is preferably 30 degree.
Described sheath stream chamber body is the shape of rectangular parallelepiped or square normally.
Described sheath stream chamber body is made by crystalline ceramics, organic glass or optical glass.
Compared with prior art, the feature of sheath flow device of the present invention is:
1, guarantee haemocyte sample under the effect of the fluid-focus of sheath flow device, form sheath flow and make haemocyte single-row one by one pass through test section;
2, by directly flow two termination constant current sources of chamber at sheath, when individual cells passes through test section, according to impedance ratio juris, can obtain a potential pulse V who is caused by this cell and be recorded, thus the testing impedance data of acquisition individual cells;
When 3, individual cells is by test section, when recording the potential pulse being caused by this cell also by Ear Mucosa Treated by He Ne Laser Irradiation, thereby also can record anterior angle scattered light (A place) signal S0, narrow angle scattered light (B place) the signal S10 of this cell, the test data of 90 degree scattered light (C place) signal S90,90 degree depolarization scattered light (D place) signal S90D, these 5 groups of data can better be screened and count specific cells;
4, optical detection function element and impedance detection function element unite two into one, and volume is less, and reliability is higher.
Accompanying drawing explanation
Fig. 1 is the perspective view of a kind of embodiment of cellanalyzer sheath flow device of the present invention;
Fig. 2 is the cut-away view of Fig. 1 illustrated embodiment;
Fig. 3 is the cut-away view of Fig. 1 illustrated embodiment mesotheca stream chamber;
Fig. 4 is the fundamental diagram of sheath stream chamber;
Fig. 5 is the light path system figure with cellanalyzer sheath flow device of the present invention coupling.
Number in the figure is:
1 sheath stream chamber, 101 inflow chambers; 102 test chambers; 103 exhaust chambers; 2 sheath flow device seats; 3 sheath flow device lids; 4 samples add pin; 5 side pipes; 6 sleeve pipes; 7 needle stands; 8 first catoptrons; 9 cylindrical mirrors; 10 second catoptrons; 11 little slits; 12 imaging balsaming lenss; 13 forward direction condensers; 14 perforation catoptrons; 1501 photoelectric cell A; 1502 photoelectric cell B; 16 micro-gummed mirrors; 17 large slits; 18 plano-concaves, plano-convex convergent lens; 19 spectroscopes; 20 polaroid filters; 21 photomultiplier A; 22 photomultiplier B; 23 semiconductor lasers; 24 sheath flow devices; H represents the height of test chamber; The bore of W test chamber.
Embodiment
As depicted in figs. 1 and 2, cellanalyzer sheath flow device of the present invention, comprise sheath flow device seat 2, be installed on the sheath stream chamber 1 on sheath flow device seat 2 tops, and the sheath flow device lid 3 that is arranged on sheath flow device seat 2 bottoms, in the inner chamber of sheath flow device seat 2, be provided with needle stand 7, needle stand 7 is separated into sheath flow device seat 2, lower two inner chambers, in the bottom of needle stand 7, be provided with 1 sleeve pipe 6 and 2 side pipes 5, sample adds pin 4 by described sleeve pipe 6, to stretch in the top inner chamber of sheath flow device seat 2, and sample adds longitudinal center's axis of the central axis of pin 4 and the test chamber 102 of sheath stream chamber 1 to coincide, 2 described side pipes 5 are for carrying sheath fluids, 2 described side pipes 5 to be positioned at the both sides of sleeve pipe 6 and with the central axis rotational symmetry setting of sleeve pipe 6 to sheath flow device seat 2 inner chambers.Described sheath stream chamber 1 comprises sheath stream chamber 1 body of the side's of being shape, on 1 body of sheath stream chamber, be provided with an inflow chamber 101, an exhaust chamber 103, and the test chamber 102 that is communicated with described inflow chamber 101 and exhaust chamber 103, described inflow chamber 101 and exhaust chamber 103 are all taper type, described test chamber 102 is square or is cylindrical, the height H of described test chamber 102 is 60～80 μ m, and its bore W is 50～100 μ m; The geometric center of described test chamber 102 overlaps with the geometric center of sheath stream chamber 1 body, described inflow chamber 101 and exhaust chamber 103 be take and be symmetrical structure through the horizontal line of the geometric center of test chamber 102 as axis of symmetry, and longitudinal center's axis that the central axis of described inflow chamber 101, the central axis of exhaust chamber 103 and longitudinal center's axis of test chamber 102 all flow chamber 1 body with sheath overlaps; Dock with top, 101 end of the inflow chamber of sheath flow device at the top of described sheath flow device seat 2 inner chambers, and both docking ports fit like a glove.
In the present embodiment, sheath stream chamber 1 body is rectangular shape, by quartz glass, is made; The angle of the extended line of two buses of inflow chamber 101 or exhaust chamber 103 is 30 degree, and the height H of test chamber 102 is 70 μ m, and its bore W is 60 μ m, and its sectional view as shown in Figure 3.
Principle of work is: two lower lumen that side pipe 5 enter sheath flow device seat 2 of sheath fluid from needle stand 7, hole on needle stand 7 enters the top inner chamber of sheath flow device seat 2 again, then enter the top of sheath flow device seat 2 top inner chambers, then enter the inflow chamber 101 (in top and the interior formation fluid-focus of inflow chamber 101 portion of sheath flow device inner chamber) of the sheath stream chamber 1 being connected with sheath flow device seat 2 top inner chambers, and cell sample adds pin 4 to enter from sample, in inflow chamber 101, under the effect of fluid-focus, form sheath flow and make haemocyte single-row one by one pass through test chamber 102.The two ends of flowing chamber 1 due to sheath are connected to constant current source, and therefore, when individual cells passes through test section, according to impedance ratio juris, can obtain a potential pulse V who is caused by this cell and be recorded, thus the testing impedance data of acquisition individual cells, and when this cell passes through test section, also (identical with conventional prior art with the light path system of sheath flow device coupling of the present invention by Ear Mucosa Treated by He Ne Laser Irradiation when recording the potential pulse being caused by this cell, specifically can be used in conjunction with the URIT-5500 blood analyser that the applicant produces, this light path system figure specifically as shown in Figure 5), thereby also can record anterior angle scattered light (A place) the signal S0 of this cell, narrow angle scattered light (B place) signal S10, 90 degree scattered light (C place) signal S90, the test data of 90 degree depolarization scattered light (D place) signal S90D, as shown in Figure 4, by these 5 groups of data, remove and can obtain S0-S10 coordinate diagram, S90-S90D coordinate diagram, S0-S90-S90D coordinate diagram is distinguished cell, can also obtain V-SO, V-S90, V-S90D, the coordinate diagram of V-S90-S90D is distinguished cell, thereby can to specific cells, screen better, counting.
Fig. 5 for the light path system of sheath flow device coupling of the present invention (being the light path system that URIT-5500 blood analyser that the applicant produces is used), in figure, the laser beam that semiconductor laser 23 produces enters cylindrical mirror 9 through the reflected light of the first catoptron 8 reflections, through the second catoptron 10 reflections, enter little slit 11 more afterwards, by little slit 11 laser out, after imaging balsaming lens 12, incide sheath flow device 24, incide the Ear Mucosa Treated by He Ne Laser Irradiation of sheath flow device 24 process shapings to flowing through on the cell of sheath flow device 24, cell produces scattered light, this light path is collected the scattered light information of 2 directions: forward scattering light and 90 ° of scattered lights.
The light path flow process of forward scattering light is: forward scattering light arrives perforation catoptron 14 by forward direction condenser 13, perforation catoptron 14 is divided into 2 bundles by the light beam of incident, a branch of is that 0 ° of light directly arrives photoelectric cell A1501 by perforation catoptron 14, and 10 ° of light of another bundle arrive photoelectric cell B1502 through perforation catoptron 14.
The light path flow process of 90 ° of scattered lights is: 90 ° of scattered lights are by the large slit 17 of the rear arrival of micro-gummed mirror 16, by arriving plano-concave, plano-convex convergent lens 18 after large slit 17 shapings, light beam arrives spectroscope 19 by plano-concave, plano-convex convergent lens 18 after assembling, spectroscope 19 is divided into 2 bundles by incident light, a branch of photomultiplier B21 that directly enters, another bundle is by the rear arrival photomultiplier of polaroid filter 20 A21.
Claims (5)
1. a cellanalyzer sheath flow device, the sheath stream chamber (1) and the sheath flow device that comprise sheath flow device seat (2), are installed on respectively sheath flow device seat (2) two ends cover (3), sample adds pin (4) to be installed in the inner chamber of sheath flow device seat (2) by needle stand (7), on described needle stand (7), be also provided with two side pipes (5) that add pin (4) central shaft rotational symmetry to arrange with sample, it is characterized in that:
Described sheath stream chamber (1) comprises sheath stream chamber (1) body of the side's of being shape, on the body of sheath stream chamber (1), be provided with an inflow chamber (101), an exhaust chamber (103), and the test chamber (102) that is communicated with described inflow chamber (101) and exhaust chamber (103), described inflow chamber (101) and exhaust chamber (103) are all taper type, described test chamber (102) is square or is cylindrical, the height (H) of described test chamber (102) is 60～80 μ m, and its bore (W) is 50～100 μ m; The geometric center of described test chamber (102) overlaps with the geometric center of sheath stream chamber (1) body, the horizontal line that described inflow chamber (101) and exhaust chamber (103) be take through the geometric center of test chamber (102) is symmetrical structure as axis of symmetry, and longitudinal center's axis that the central axis of described inflow chamber (101), the central axis of exhaust chamber (103) and longitudinal center's axis of test chamber (102) all flow chamber (1) body with sheath overlaps.
2. cellanalyzer sheath flow device according to claim 1, is characterized in that: the height (H) of described test chamber (102) is 70 μ m, and its bore (W) is 60 μ m.
3. cellanalyzer sheath flow device according to claim 1 and 2, is characterized in that: the angle of the extended line of two buses of described inflow chamber (101) or exhaust chamber (103) is 30 degree.
4. cellanalyzer sheath flow device according to claim 1 and 2, is characterized in that: described sheath stream chamber (1) body is rectangular parallelepiped or square.
5. cellanalyzer sheath flow device according to claim 1 and 2, is characterized in that: described sheath stream chamber (1) body is made by crystalline ceramics, organic glass or optical glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310560181.9A CN103575636A (en) | 2013-11-12 | 2013-11-12 | Sheath flow device of hematology analyzer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310560181.9A CN103575636A (en) | 2013-11-12 | 2013-11-12 | Sheath flow device of hematology analyzer |
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| CN103575636A true CN103575636A (en) | 2014-02-12 |
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| CN201310560181.9A Pending CN103575636A (en) | 2013-11-12 | 2013-11-12 | Sheath flow device of hematology analyzer |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106226223A (en) * | 2016-08-24 | 2016-12-14 | 桂林优利特医疗电子有限公司 | Particle analyzer |
| CN106769805A (en) * | 2017-01-22 | 2017-05-31 | 江西特康科技有限公司 | Cell count sorter |
| CN107843542A (en) * | 2017-11-27 | 2018-03-27 | 桂林优利特医疗电子有限公司 | The specimen cup that a kind of cellanalyzer uses |
| CN108663306A (en) * | 2018-07-27 | 2018-10-16 | 北京指真生物科技有限公司 | A kind of flow cytometry device and compensation method |
| CN109060749A (en) * | 2018-08-22 | 2018-12-21 | 桂林优利特医疗电子有限公司 | A kind of Optical devices for cell count and detection |
| CN110832302A (en) * | 2017-06-28 | 2020-02-21 | 迪亚戈达夫公司 | Measuring cuvette for counting and/or characterizing cells |
| WO2020146967A1 (en) * | 2019-01-14 | 2020-07-23 | 深圳迈瑞生物医疗电子股份有限公司 | Optical test device for sample |
| CN114112919A (en) * | 2020-08-31 | 2022-03-01 | 深圳市帝迈生物技术有限公司 | Optical flow cell assembly, optical detection device and sample analysis apparatus |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114112919A (en) * | 2020-08-31 | 2022-03-01 | 深圳市帝迈生物技术有限公司 | Optical flow cell assembly, optical detection device and sample analysis apparatus |
| CN114112919B (en) * | 2020-08-31 | 2024-04-05 | 深圳市帝迈生物技术有限公司 | Optical flow cell assembly, optical detection device and sample analysis device |
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Application publication date: 20140212 |