CN109060749A - A kind of Optical devices for cell count and detection - Google Patents
A kind of Optical devices for cell count and detection Download PDFInfo
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- CN109060749A CN109060749A CN201810961915.7A CN201810961915A CN109060749A CN 109060749 A CN109060749 A CN 109060749A CN 201810961915 A CN201810961915 A CN 201810961915A CN 109060749 A CN109060749 A CN 109060749A
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- 230000003287 optical effect Effects 0.000 title claims abstract description 36
- 238000001514 detection method Methods 0.000 title claims abstract description 30
- 238000007493 shaping process Methods 0.000 claims abstract description 24
- 230000005540 biological transmission Effects 0.000 claims abstract description 20
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 24
- 230000005499 meniscus Effects 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 12
- 239000000835 fiber Substances 0.000 claims description 11
- 230000008878 coupling Effects 0.000 claims description 5
- 238000010168 coupling process Methods 0.000 claims description 5
- 238000005859 coupling reaction Methods 0.000 claims description 5
- 230000005622 photoelectricity Effects 0.000 claims 1
- 238000004458 analytical method Methods 0.000 abstract description 10
- 210000004027 cell Anatomy 0.000 description 33
- 239000002245 particle Substances 0.000 description 9
- 230000006872 improvement Effects 0.000 description 5
- 239000011148 porous material Substances 0.000 description 5
- 239000013307 optical fiber Substances 0.000 description 4
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- 230000013011 mating Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000009738 saturating Methods 0.000 description 2
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
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- 230000003760 hair shine Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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Abstract
The present invention discloses a kind of Optical devices for cell count and detection, and sheath flow device is located on the optical path joint of light source shaping unit, fluorescence signal receiving unit and 0 degree and 10 degree of signal receiving unit this 3 units；Wherein the incidence end of the exit end of light source shaping unit and sheath flow device connects, one of exit end of sheath flow device is connect with the incidence end of 0 degree and 10 degree signal receiving unit, another exit end of sheath flow device and the incidence end of light element connect, and the exit end of light element is connect with the incidence end of fluorescence signal receiving unit.The present invention is designed by the Optical devices to cell analysis instrument, is improved the utilization efficiency and fluorescence transfer efficiency of exciting light, is shortened the transmission range of fluorescence, reduce the loss of fluorescence signal, improve the utilization rate of light, to improve accuracy in detection.
Description
Technical field
The present invention relates to cell analysis technical fields, and in particular to a kind of Optical devices for cell count and detection.
Background technique
Currently, the instrument for cell count and detection has flow cytometer, cellanalyzer, leukocyte differential count to detect
Instrument etc., it is fast that these instruments are all based on the light scattering technique progress multi-parameters such as cell volume size and cell interior complexity
Fast quantitative analysis, to realize the cell analysis instrument analyzed specific cell colony and sorted.These cell analysis instruments
Working principle be: laser light source by multiple lens carry out shaping focusing, compression spot size to certain size range in,
And expose to laser beam on the pore in sheath flow device by reflecting mirror, when laser beam is radiated at capillary by sheath flow device
Scattered light signal can be generated when on the cell in hole, be then collected by lens on light signal, focussed collimated propagates to light
Electric multiplier tube or photodiode carry out the reception and conversion of optical signal, and electric signal sent into host computer carry out it is a series of
Signal processing realizes the signature analysis to cell.When analyzing due to host computer cell, it is all based on cell scattering light letter
It number realizes, therefore the light path system of cell analysis instrument determines the performance of cell analysis instrument, light path system is designed
More reasonable, the loss of cell scattered light signal is smaller, and the signal-to-noise ratio of cell scattered light signal is higher, the detection knot of cell analysis instrument
Fruit is then more accurate.
Summary of the invention
The present invention is intended to provide a kind of Optical devices for cell count and detection, can reduce cell scattering light letter
Number loss, improve the signal-to-noise ratio of scattered light signal, realize the accurate detection of eucaryotic cell structure and bulk properties.
To solve the above problems, the present invention is achieved by the following technical solutions:
A kind of Optical devices for cell count and detection, mainly by pedestal, and the light source being mounted on the base is whole
Shape unit, sheath flow device, 0 degree and 10 degree of signal receiving units, light element, fluorescence signal receiving unit composition；Sheath flow device is located at
On the optical path joint of light source shaping unit, fluorescence signal receiving unit and 0 degree and 10 degree of signal receiving unit this 3 units；
Wherein the incidence end of the exit end of light source shaping unit and sheath flow device connects, one of exit end of sheath flow device and 0 degree and 10
The incidence end connection of signal receiving unit is spent, another exit end of sheath flow device and the incidence end of light element connect, guide-lighting single
The exit end of member is connect with the incidence end of fluorescence signal receiving unit.
In above scheme, fluorescence signal receiving unit receives shell by signal, and is located at signal and receives intracorporal 5, shell
Fluorescence dichroscope and 6 photoelectric converter compositions；The incidence end of first fluorescence dichroscope forms fluorescence signal receiving unit
Incidence end；The reflection output end of first fluorescence dichroscope is connect with the first photoelectric converter；First fluorescence dichroscope
Transmission output end is connect with the incidence end of the second fluorescence dichroscope；The reflection output end of second fluorescence dichroscope and the 5th glimmering
The incidence end of light dichroscope connects, the incidence of the transmission output end and third light fluorescence dichroscope of the second fluorescence dichroscope
End connection；The reflection output end of 5th fluorescence dichroscope is connect with the 6th photoelectric converter；5th fluorescence dichroscope it is saturating
Output end is penetrated to connect with the 5th photoelectric converter；The incident output end of third light fluorescence dichroscope connect the 4th smooth fluorescence two to
The incidence end of Look mirror；The transmission output end of third light fluorescence dichroscope is connect with the second photoelectric converter；4th smooth fluorescence two
It is connect to the reflection output end of Look mirror with third photoelectric converter；The transmission output end and the 4th light of 4th smooth fluorescence dichroscope
Electric transducer connection.
As an improvement, the output end of fluorescence dichroscope is connect by bandpass filter with photoelectric converter.
It is received in shell as an improvement, fluorescence dichroscope is movably mounted to signal using plug type.
In above scheme, light element is by guide-lighting pedestal, preceding guide-lighting meniscus lens, rear guide-lighting meniscus lens, cemented doublet, aobvious
The micro- eyepiece peace of micro- lens barrel, slit baffle, plano-concave highlights micro- eyepiece composition；Guide-lighting through-hole is offered in guide-lighting pedestal；
The entrance of guide-lighting through-hole forms the incidence end of light element, and towards sheath flow device；The outlet of guide-lighting through-hole forms light element
Exit end, and towards fluorescence signal receiving unit；Preceding leaded light meniscus lens, rear guide-lighting meniscus lens, cemented doublet and microcobjective
Mirror is sequentially arranged in the inlet of guide-lighting through-hole from front to back, and preceding guide-lighting meniscus lens are positioned close to the entrance one of guide-lighting through-hole
Side, microcobjective mirror are positioned close to the outlet side of guide-lighting through-hole；The micro- eyepiece peace of slit baffle, plano-concave highlights micro- eyepiece
It is sequentially arranged in the exit of guide-lighting through-hole from front to back, and slit baffle is positioned close to the inlet side of guide-lighting through-hole, puts down
Highlight the outlet side that micro- eyepiece is positioned close to guide-lighting through-hole.
In above scheme, light element is fiber optic bundle, and one end of the fiber optic bundle is coupled with sheath flow device, and the other end and fluorescence are believed
The incidence end coupling of number receiving unit.
As an improvement, having additional a plano-convex lens between fiber optic bundle and sheath flow device.
In above scheme, 0 degree and 10 degree of signal receiving units receive pedestal, diaphragm, forward direction condenser, perforation by first
Mirror, 0 degree of signal receiving circuit plate and 10 degree of signal receiving circuit board groups at；First reception pedestal in offer be connected enter light
Through-hole, 0 degree of light through-hole out of light through-hole and 10 degree out；0 degree out light through-hole coincide with the central axes for entering light through-hole, 10 degree out light it is logical
Hole and the central axes for entering light through-hole are perpendicular, and the central axes intersection for entering light through-hole, 0 degree of light through-hole out of light through-hole and 10 degree out exists
The intersection of this 3 through-holes；Perforated mirror is arranged in the intersection, and enter the outlet of light through-hole by perforated mirror with 0 degree out light lead to
The entrance of the light through-hole out of the entrance in hole and 10 degree is connected；The entrance for entering light through-hole forms entering for 0 degree and 10 degree signal receiving unit
End is penetrated, and towards sheath flow device, and the inlet into light through-hole is arranged in forward direction condenser；0 degree of signal receiving circuit plate is arranged 0
Spend the exit of light through-hole out, the exit of 10 degree of light through-holes out is arranged in 10 degree of signal receiving circuit plates.
As an improvement, the front side of forward direction condenser is equipped with diaphragm.
In above scheme, light source shaping unit is mainly by 2 lasers, 4 cylindrical mirrors, 1 reflecting mirror, 1 light source two
To Look mirror and 1 light-resource fousing lens composition；2 lasers have different wavelength, and one of laser is mutual by 2
It is incident on reflecting mirror after orthogonal cylindrical mirror, and is incident on after the reflection of reflecting mirror one of them of light source dichroscope and enters
Penetrate end；Another laser, which then passes through, to be directly incident on another of light source dichroscope after 2 mutually orthogonal cylindrical mirrors and enters
Penetrate end；The incidence end of the exit end connection light-resource fousing lens of light source dichroscope, the exit end of light-resource fousing lens form light
The exit end of source shaping unit.
As an improvement, sheath flow device can be realized the orientation adjustable plate for realizing the adjusting of X-axis, Y-axis and Z-direction by one
It is mounted on the base.
Compared with prior art, the present invention has a characteristic that
1, it is designed by the Optical devices to cell analysis instrument, the utilization efficiency and fluorescence for improving exciting light pass
Efficiency is passed, the transmission range of fluorescence is shortened, reduces the loss of fluorescence signal, improves the utilization rate of light, to improve
Accuracy in detection.
2, it is shone using bidifly and carries out the detection of cell, the multifrequency nature of a cell can analyzed, improve detection
Efficiency；
3, the fluorescence dichroscope of fluorescence signal receiving unit is designed using plug type, convenient for multiple fluorescence channels from
By replacing, convenient for channel configuration change and flexible upgrading, use cost and maintenance cost greatly have been saved for user；
4, light element is scattered the collection of fluorescence signal using light transmission type optical fiber, can reduce optical path to a certain extent
Structure, so that equipment instrument minimizes, cost is more cheaper, and the low damage of optical signal is realized using optical fiber total reflection characteristic
Consumption.
Detailed description of the invention
Fig. 1 is a kind of schematic perspective view of Optical devices for cell count and detection；
Fig. 2 is a kind of top view of Optical devices for cell count and detection；
Fig. 3 is a kind of light path principle figure of Optical devices for cell count and detection；
Fig. 4 is the schematic perspective view of light source shaping unit；
Fig. 5 is the sectional axonometric drawing of 0 degree and 10 degree signal receiving unit；
Fig. 6 is a kind of sectional axonometric drawing (embodiment one) of light element；
Fig. 7 is the sectional view of fluorescence signal receiving unit (removing photoelectric converter)；
Fig. 8 is the light path principle figure of fluorescence signal receiving unit；
Fig. 9 is the scheme of installation (embodiment two) of another light element；
Figure label: 1, pedestal；2, light source shaping unit, 2-1 laser, 2-2, cylindrical mirror, 2-3, reflecting mirror, 2-4, light
Source dichroscope, 2-5, light-resource fousing lens；3, sheath flow device；4,4-1,0 degree and 10 degree of signal receiving units receive pedestal, 4-
1-1, enter light through-hole, 4-1-2,0 degree of light through-hole out, 4-1-3,10 degree of light through-holes out, 4-2, diaphragm, 4-3, forward direction condenser, 4-
4, perforated mirror, 4-5,0 degree of signal receiving circuit plate, 4-6,10 degree of signal receiving circuit plates；5, light element, 5-1, guide-lighting base
Seat, 5-1-1, guide-lighting through-hole, 5-2, preceding guide-lighting meniscus lens, 5-3, rear guide-lighting meniscus lens, 5-4, cemented doublet, 5-5, micro- object
Mirror lens barrel, 5-6, microcobjective regulating part, 5-7, slit baffle, the micro- eyepiece of 5-8, plano-concave, the micro- eyepiece of 5-9, plano-convex, 5-
10, fiber optic bundle；6, fluorescence signal receiving unit, 6-1, signal receive shell, 6-2, fluorescence dichroscope, 6-3, bandpass filter
Piece, 6-4, photoelectric converter.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific example, and referring to attached
Figure, the present invention is described in more detail.It should be noted that the direction term mentioned in example, for example, "upper", "lower",
" in ", " left side " " right side ", "front", "rear" etc., be only the direction with reference to attached drawing.Therefore, the direction used is intended merely to explanation
For limiting the scope of the invention.
Embodiment one:
Referring to Fig. 1-3, a kind of Optical devices for cell count and detection mainly by pedestal 1, and are mounted on pedestal
3,0 degree of light source shaping unit 2, sheath flow device and 10 degree of signal receiving units 4, light element 5, fluorescence signal receiving unit 6 on 1
Composition.Sheath flow device 3 is located at light source shaping unit 2, fluorescence signal receiving unit 6 and 0 degree and 10 degree of signal receiving units 4 this 3
On the optical path joint of unit.Wherein the exit end of light source shaping unit 2 is connect with the incidence end of sheath flow device 3, sheath flow device 3 its
In an exit end connect with the incidence end of 0 degree and 10 degree signal receiving unit 4, another exit end of sheath flow device 3 and guide-lighting
The incidence end of unit 5 is connected with exit end, and the exit end of light element 5 is connect with the incidence end of fluorescence signal receiving unit 6.Light
The laser beam of the output of source shaping unit 2 shines directly on the pore in sheath flow device 3.Laser beam is passing through sheath flow device
3 when being radiated on the cell in pore, generates scattered light signal.A part in the scattered light signal enters directly into 0 degree
And in 10 degree of signal receiving units 4, another part in scattered light signal enters fluorescence signal receiving unit by light element 5
6.0 degree and 10 degree of signal receiving units 4 and fluorescence signal receiving unit 6 send into host computer after being collected to optical signal and carry out
Cell analysis.
Above-mentioned light source shaping unit 2 is mainly by 2 laser 2-1,4 cylindrical mirror 2-2,1 reflecting mirror 2-3,1 light source
Dichroscope 2-4 and 1 light-resource fousing lens 2-5 composition.2 laser 2-1 have different wavelength.In the present embodiment,
One of laser 2-1 is the red laser 2-1 of 635nm, another laser 2-1 is the blue laser 2- of 488nm
1.One of laser 2-1 passes through reflecting mirror 2- by being incident on reflecting mirror 2-3 after 2 mutually orthogonal cylindrical mirror 2-2
One of incidence end of light source dichroscope 2-4 is incident on after 3 reflection.Another laser 2-1 is mutually orthogonal by 2
Cylindrical mirror 2-2 after be directly incident on another incidence end of light source dichroscope 2-4.The exit end of light source dichroscope 2-4
It is incident in the pore of sheath flow device 3 via light-resource fousing lens 2-5.The round laser facula that one laser 2-1 launches
After 2 mutually orthogonal cylindrical mirror 2-2, circular light spot will be compressed into one by cylindrical mirror 2-2 has specific dimensions
Elliptical spot, the subsequent elliptical spot are radiated on reflecting mirror 2-3, and passed through after reflecting mirror 2-3 reflection light source two to
Look mirror 2-4 is focused on sheath flow device 3 finally by light-resource fousing lens 2-5, carries out detection of particles.And at the same time, another
For the round laser facula that laser 2-1 launches after 2 mutually orthogonal cylindrical mirror 2-2, circular light spot will be by cylinder
Mirror 2-2 is compressed into the elliptical spot with specific dimensions, and the subsequent elliptical spot is directly through light source dichroscope
2-4, and focused on sheath flow device 3 by light-resource fousing lens 2-5, carry out detection of particles.It can for spot shaping cylindrical mirror 2-2
Other than laser 2-1 light-emitting window, while shaping cylindrical mirror 2-2 is also mountable within laser 2-1, as integral type shaping
Laser 2-1.Referring to fig. 4.
There is cell to be measured in the pore of above-mentioned sheath flow device 3.Sheath flow device 3 is mounted on pedestal 1 by an orientation adjustable plate
On, which is able to drive the movement that sheath flow device 3 realizes X-axis, Y-axis and Z-direction, to guarantee the capillary of sheath flow device 3
Hole is located exactly at light source shaping unit 2, fluorescence signal receiving unit 6 and 0 degree and 10 degree of signal receiving unit 4 this 3 units
On optical path joint.The elliptical spot with specific dimensions that light source shaping unit 2 is emitted is radiated to be checked in sheath flow device 3
When particle, light beam will generate 360 ° of light scattering along particle, and be believed respectively by fluorescence signal receiving unit 6 and 0 degree and 10 degree
Number receiving unit 4 receives.
Above-mentioned 0 degree and 10 degree of signal receiving units 4 are by reception pedestal 4-1, diaphragm 4-2, forward direction condenser 4-3, perforated mirror
4-4,0 degree of signal receiving circuit plate 4-5 and 10 degree of signal receiving circuit plate 4-6 compositions.It receives to offer in pedestal 4-1 and be connected
Enter light through hole socket 4-1-1,0 degree of light through-hole 4-1-3 out of light through-hole 4-1-2 and 10 degree out.0 degree light through-hole 4-1-2 and enters light out
The central axes of through hole socket 4-1-1 coincide, 10 degree out light through-hole 4-1-3 and the central axes for entering light through hole socket 4-1-1 it is perpendicular, and
Enter light through hole socket 4-1-1, the central axes of 0 degree of light through-hole 4-1-3 out of light through-hole 4-1-2 and 10 degree out are intersected in this 3 through-holes
Intersection.Perforated mirror 4-4 is arranged in the intersection.Enter the outlet of light through hole socket 4-1-1 by perforated mirror 4-4 with 0 degree out light lead to
The entrance of the light through-hole 4-1-3 out of the entrance of hole 4-1-2 and 10 degree is connected.Enter the entrance of light through hole socket 4-1-1 towards sheath flow device 3.
The inlet into light through hole socket 4-1-1 is arranged in forward direction condenser 4-3.Diaphragm 4-2 be placed in front of to the front side of condenser 4-3,
Main function is to block the light beam that diffraction, scattering phenomenon does not occur with particle, thus promotes the reliability of detection, and reduce signal
The background voltage of circuit board for receiving.The exit of 0 degree of light through-hole 4-1-2 out is arranged in 0 degree of signal receiving circuit plate 4-5.10 degree
The exit of 10 degree of light through-hole 4-1-3 out is arranged in signal receiving circuit plate 4-6.To poly- before 3 scattered beam of sheath flow device passes through
When light microscopic 4-3, scattered beam will gradually be assembled, and a part of low-angle light will be by perforated mirror 4-4, and passes through 0 degree out
Light through-hole 4-1-2 is propagated on 0 degree of signal receiving circuit plate 4-5, and the light of another part wide-angle will be by perforated mirror 4-4
Reflection, and by 10 degree out light through-hole 4-1-3 propagate on 10 degree of signal receiving circuit plate 4-6, this completes optical signals 0
Degree and the signal in 10 degree of directions receive.Referring to Fig. 5.
It, will when on the cell particles specifically bound with fluorescent particle when laser beam is radiated in sheath flow device 3
It will do it fluorescence excitation and generate Stokes frequency domain, cause light beam wavelength to infrared direction transition.Therefore with light beam advance side
Fluorescence signal is imported into fluorescence signal receiving unit 6 to one light element 5 of setting.In the present embodiment, light element 5
Using micro- collimation lens set, mainly by guide-lighting pedestal 5-1, preceding guide-lighting meniscus lens 5-2, rear guide-lighting meniscus lens 5-3, double gluings
Lens 5-4, microcobjective lens barrel 5-5, slit baffle 5-7, the micro- eyepiece 5-8 peace of plano-concave highlight micro- eyepiece 5-9 composition.It is guide-lighting
Guide-lighting through-hole 5-1-1 is offered in pedestal 5-1.Towards sheath flow device 3, guide-lighting through-hole 5-1-1's goes out the entrance of guide-lighting through-hole 5-1-1
Mouthful towards fluorescence signal receiving unit 6.Preceding leaded light meniscus lens 5-2, rear guide-lighting meniscus lens 5-3, cemented doublet 5-4 and micro- object
Mirror mirror is sequentially arranged in the inlet of guide-lighting through-hole 5-1-1 from front to back, and preceding leaded light meniscus lens 5-2 is positioned close to guide-lighting lead to
The inlet side of hole 5-1-1, microcobjective mirror are positioned close to the outlet side of guide-lighting through-hole 5-1-1.Slit baffle 5-7, it puts down
Recessed micro- eyepiece 5-8 peace highlights the exit that micro- eyepiece 5-9 is sequentially arranged in guide-lighting through-hole 5-1-1 from front to back, and slit
Baffle 5-7 is positioned close to the inlet side of guide-lighting through-hole 5-1-1, and the micro- eyepiece 5-9 of plano-convex is positioned close to guide-lighting through-hole 5-
The outlet side of 1-1.It is 1~3 degree and 7~11 degree of two light that the effect of slit baffle 5-7, which is by forward-scattering signal beam splitting,
Component.Microcobjective regulating part 5-6 is had on microcobjective lens barrel 5-5, is adjusted to the focus to microcobjective lens barrel 5-5
Section.The focal length of micro-imaging lens group is 35~100mm.The fluorescence signal that sheath flow device 3 scatters is first successively by guide-lighting single
Preceding guide-lighting meniscus lens 5-2, rear guide-lighting meniscus lens 5-3, cemented doublet 5-4 and the microcobjective lens barrel 5-5 of first 5 inlet, so
Successively pass through the slit baffle 5-7 of 5 inlet of light element afterwards, the micro- eyepiece 5-8 peace of plano-concave highlights micro- eyepiece 5-9, finally
Collimation focusing is in the fluorescence signal receiving unit 6 of rear end.Referring to Fig. 6.
Above-mentioned fluorescence signal receiving unit 6 receives shell 6-1 by signal, and 5 be located in signal reception shell 6-1
Fluorescence dichroscope 6-2,6 bandpass filter 6-3 and 6 photoelectric converter 6-4 compositions.All fluorescence dichroscope 6-2 are adopted
Signal is movably mounted to plug type to receive in shell 6-1.All fluorescence dichroscope 6-2 and fluorescent scattering signal key light
The angular range of line is 25~50 °.Signal, which receives, is equipped with extinction cotton in shell 6-1, can reflect fluorescence dichroscope 6-2
It will be irradiated on extinction cotton with wavelength remaining after transmission, and avoid unnecessary photosignal and generate.5 fluorescence dichroics
Mirror 6-2 has different transmission peak wavelengths, and fluorescence dichroscope 6-2 is mounted on signal at 45 degree of angles and receives in shell 6-1, main
Effect is to separate the light beam of different wave length, by reflecting back along 90 ° of directions for specific wavelength, and allows specific wavelength
Through.When light has stepped through fluorescence dichroscope 6-2, light intensity will gradually weaken, while entrained particle information
It will gradually reduce.The incidence end of first fluorescence dichroscope 6-2 forms the incidence end of fluorescence signal receiving unit 6.5 band logical filters
Mating plate 6-3 has different filter wavelengths, and main function is that non-specific wavelength is stopped to pass through, and only specific wavelength is allowed to pass through,
Thus play the role of clean signal.Bandpass filter 6-3 is being received in shell using that can intercut formula case.Photoelectric converter 6-
4 main functions are to convert optical signals into electric signal output, and be sent to host computer and show particle properties entrained by it.
In the present embodiment, photoelectric converter 6-4 can be photomultiplier tube, avalanche photodide, photodiode or other electricity
Lotus coupling element.
The reflection output end of first fluorescence dichroscope 6-2 is via the first bandpass filter 6-3 and the first photoelectric converter
6-4 connection, the fluorescent scattering signal chief ray after being excited are incident on the first fluorescence dichroscope 6-2 with 45° angle.First is glimmering
The transmission output end of light dichroscope 6-2 is connect with the incidence end of the second fluorescence dichroscope 6-2.Second fluorescence dichroscope 6-
2 reflection output end is connect with the incidence end of the 5th fluorescence dichroscope 6-2, the transmission output of the second fluorescence dichroscope 6-2
End is connect with the incidence end of third light fluorescence dichroscope 6-2.The reflection output end of 5th fluorescence dichroscope 6-2 is via the 6th
Bandpass filter 6-3 is connect with the 6th photoelectric converter 6-4.The transmission output end of 5th fluorescence dichroscope 6-2 is via the 5th
Bandpass filter 6-3 is connect with the 5th photoelectric converter 6-4.The incident output end connection the of third light fluorescence dichroscope 6-2
The incidence end of four smooth fluorescence dichroscope 6-2.The transmission output end of third light fluorescence dichroscope 6-2 is via the second bandpass filter
Piece 6-3 is connect with the second photoelectric converter 6-4.The reflection output end of 4th smooth fluorescence dichroscope 6-2 is filtered via third band logical
Mating plate 6-3 is connect with third photoelectric converter 6-4.The transmission output end of 4th smooth fluorescence dichroscope 6-2 is via the 4th band logical
Optical filter 6-3 is connect with the 4th photoelectric converter 6-4.Referring to Fig. 7 and 8.
Embodiment two:
Embodiment two and the structure of the Optical devices of embodiment one are substantially the same, difference be light element 5 using
One end of fiber optic bundle 5-10, rather than micro- collimation lens set, fiber optic bundle 5-10 are coupled with sheath flow device 3, and the other end and fluorescence are believed
The incidence end coupling of number receiving unit 6.Referring to Fig. 9.It can be realized micro- damage of optical signal using Y type light transmission type fiber optic bundle 5-10
Consumption, general standard single mode optical fiber are 0.2dB/Km in the loss factor of 1550nm.Fiber-optic signal receiving end is at a distance from sheath flow device 3
For 2~10mm.In addition, also having additional the plano-convex lens of a large-numerical aperture between fiber optic bundle 5-10 and sheath flow device 3, plano-convex is saturating
The main function of mirror is to collect light beam, preferably enters light beam coupling in optical fiber.
It should be noted that although the above embodiment of the present invention be it is illustrative, this be not be to the present invention
Limitation, therefore the invention is not limited in above-mentioned specific embodiment.Without departing from the principles of the present invention, all
The other embodiment that those skilled in the art obtain under the inspiration of the present invention is accordingly to be regarded as within protection of the invention.
Claims (10)
1. a kind of Optical devices for cell count and detection, characterized in that mainly by pedestal (1), and be mounted on pedestal
(1) light source shaping unit (2), sheath flow device (3), 0 degree and 10 degree of signal receiving units (4), light element (5) and fluorescence letter on
Number receiving unit (6) composition；
Sheath flow device (3) is located at light source shaping unit (2), fluorescence signal receiving unit (6) and 0 degree and 10 degree of signal receiving units
(4) on the optical path joint of this 3 units；Wherein the incidence end of the exit end of light source shaping unit (2) and sheath flow device (3) connects
It connects, one of exit end of sheath flow device (3) is connect with the incidence end of 0 degree and 10 degree signal receiving unit (4), sheath flow device (3)
Another exit end connect with the incidence end of light element (5), the exit end of light element (5) and fluorescence signal receiving unit
(6) incidence end connection.
2. a kind of Optical devices for cell count and detection according to claim 1, characterized in that fluorescence signal connects
It receives unit (6) and shell (6-1) is received by signal, and 5 fluorescence dichroscope (6- being located in signal reception shell (6-1)
2) it is formed with 6 photoelectric converters (6-4)；
The incidence end of first fluorescence dichroscope (6-2) forms the incidence end of fluorescence signal receiving unit (6)；First fluorescence two to
The reflection output end of Look mirror (6-2) is connect with the first photoelectric converter (6-4)；The transmission of first fluorescence dichroscope (6-2) is defeated
Outlet is connect with the incidence end of the second fluorescence dichroscope (6-2)；The reflection output end of second fluorescence dichroscope (6-2) and
The incidence end of five fluorescence dichroscopes (6-2) connects, the transmission output end and third light fluorescence of the second fluorescence dichroscope (6-2)
The incidence end of dichroscope (6-2) connects；The reflection output end and the 6th photoelectric converter of 5th fluorescence dichroscope (6-2)
(6-4) connection；The transmission output end of 5th fluorescence dichroscope (6-2) is connect with the 5th photoelectric converter (6-4)；Third light is glimmering
The incident output end of light dichroscope (6-2) connects the incidence end of the 4th smooth fluorescence dichroscope (6-2)；Third light fluorescence two to
The transmission output end of Look mirror (6-2) is connect with the second photoelectric converter (6-4)；The reflection of 4th smooth fluorescence dichroscope (6-2)
Output end is connect with third photoelectric converter (6-4)；The transmission output end and the 4th photoelectricity of 4th smooth fluorescence dichroscope (6-2)
Converter (6-4) connection.
3. a kind of Optical devices for cell count and detection according to claim 2, characterized in that fluorescence dichroic
The output end of mirror (6-2) is connect by bandpass filter (6-3) with photoelectric converter (6-4).
4. a kind of Optical devices for cell count and detection according to claim 2, characterized in that fluorescence dichroic
Mirror (6-2) is movably mounted to signal using plug type and receives in shell (6-1).
5. a kind of Optical devices for cell count and detection according to claim 1, characterized in that light element
(5) by guide-lighting pedestal (5-1), preceding guide-lighting meniscus lens (5-2), rear guide-lighting meniscus lens (5-3), cemented doublet (5-4), micro- object
Mirror lens barrel (5-5), slit baffle (5-7), the micro- eyepiece of plano-concave (5-8) peace highlight micro- eyepiece (5-9) composition；
Guide-lighting through-hole (5-1-1) is offered in guide-lighting pedestal (5-1)；The entrance of guide-lighting through-hole (5-1-1) forms light element (5)
Incidence end, and towards sheath flow device (3)；The outlet of guide-lighting through-hole (5-1-1) forms the exit end of light element (5), and direction
Fluorescence signal receiving unit (6)；Preceding leaded light meniscus lens (5-2), rear guide-lighting meniscus lens (5-3), cemented doublet (5-4) with it is micro-
Object lens mirror is sequentially arranged in the inlet of guide-lighting through-hole (5-1-1) from front to back, and preceding guide-lighting meniscus lens (5-2) are positioned close to
The inlet side of guide-lighting through-hole (5-1-1), microcobjective mirror are positioned close to the outlet side of guide-lighting through-hole (5-1-1)；Slit
Baffle (5-7), the micro- eyepiece of plano-concave (5-8) peace highlight micro- eyepiece (5-9) and are sequentially arranged in guide-lighting through-hole (5-1- from front to back
1) exit, and slit baffle (5-7) is positioned close to the inlet side of guide-lighting through-hole (5-1-1), the micro- eyepiece (5- of plano-convex
9) it is positioned close to the outlet side of guide-lighting through-hole (5-1-1).
6. a kind of Optical devices for cell count and detection according to claim 1, characterized in that light element
It (5) is fiber optic bundle (5-10) that one end of the fiber optic bundle (5-10) is coupled with sheath flow device (3), the other end and fluorescence signal receive list
The incidence end coupling of first (6).
7. a kind of Optical devices for cell count and detection according to claim 6, characterized in that fiber optic bundle (5-
10) plano-convex lens are had additional between sheath flow device (3).
8. a kind of Optical devices for cell count and detection according to claim 1, characterized in that 0 degree and 10 degree
Signal receiving unit (4) is by reception pedestal (4-1), diaphragm (4-2), forward direction condenser (4-3), perforated mirror (4-4), 0 degree of signal
Circuit board for receiving (4-5) and 10 degree of signal receiving circuit plate (4-6) compositions；
It receives and offers entering of being connected in pedestal (4-1) light through hole socket (4-1-1), 0 degree light through-hole (4-1-2) and 10 degree are out out
Light through-hole (4-1-3)；0 degree out light through-hole (4-1-2) coincide with the central axes for entering light through hole socket (4-1-1), 10 degree out light it is logical
Hole (4-1-3) and the central axes for entering light through hole socket (4-1-1) are perpendicular, and enter light through hole socket (4-1-1), 0 degree of light through-hole (4- out
1-2) and 10 degree out light through-hole (4-1-3) central axes intersection this 3 through-holes intersection；Perforated mirror (4-4) is arranged at this
Intersection, and enter the outlet of light through hole socket (4-1-1) and pass through the entrance and 10 of perforated mirror (4-4) and 0 degree of light through-hole (4-1-2) out
The entrance for spending light through-hole (4-1-3) out is connected；The entrance for entering light through hole socket (4-1-1) forms 0 degree and 10 degree of signal receiving units
(4) incidence end, and towards sheath flow device (3), and forward direction condenser (4-3) is arranged in the inlet for entering light through hole socket (4-1-1)；
The setting of 0 degree of signal receiving circuit plate (4-5) is in the exit of 0 degree of light through-hole (4-1-2) out, 10 degree of signal receiving circuit plate (4-
6) it is arranged in the exit of 10 degree of light through-holes (4-1-3) out.
9. a kind of Optical devices for cell count and detection according to claim 1, characterized in that light source shaping list
First (2) are mainly by 2 lasers (2-1), 4 cylindrical mirrors (2-2), 1 reflecting mirror (2-3), 1 light source dichroscope (2-4)
It is formed with 1 light-resource fousing lens (2-5)；
2 lasers (2-1) have different wavelength, and one of laser (2-1) passes through 2 mutually orthogonal cylindrical mirrors
It is incident on reflecting mirror (2-3) after (2-2), and is incident on light source dichroscope (2-4) its after the reflection of reflecting mirror (2-3)
In an incidence end；Another laser (2-1) is then by being directly incident on light source after 2 mutually orthogonal cylindrical mirrors (2-2)
Another incidence end of dichroscope (2-4)；Exit end connection light-resource fousing lens (2-5) of light source dichroscope (2-4)
Incidence end, the exit end of light-resource fousing lens (2-5) form the exit end of light source shaping unit (2).
10. a kind of Optical devices for cell count and detection according to claim 1, characterized in that sheath flow device (3)
The orientation adjustable plate that can be realized the adjusting for realizing X-axis, Y-axis and Z-direction by one is mounted on pedestal (1).
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| CN201810961915.7A CN109060749B (en) | 2018-08-22 | 2018-08-22 | Optical device for cell counting and detection |
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