CN209086141U - A kind of Optical devices for cell count and detection - Google Patents
A kind of Optical devices for cell count and detection Download PDFInfo
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- CN209086141U CN209086141U CN201821356284.8U CN201821356284U CN209086141U CN 209086141 U CN209086141 U CN 209086141U CN 201821356284 U CN201821356284 U CN 201821356284U CN 209086141 U CN209086141 U CN 209086141U
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Abstract
The utility model discloses a kind of Optical devices for cell count and detection, and sheath flow device is located on the optical path joint of light source shaping unit, fluorescence signal receiving unit and 0 degree and 10 degree of signal receiving unit this 3 units；Wherein the incidence end of the exit end of light source shaping unit and sheath flow device connects, one of exit end of sheath flow device is connect with the incidence end of 0 degree and 10 degree signal receiving unit, another exit end of sheath flow device and the incidence end of light element connect, and the exit end of light element is connect with the incidence end of fluorescence signal receiving unit.The utility model is designed by the Optical devices to cell analysis instrument, the utilization efficiency and fluorescence transfer efficiency for improving exciting light, shorten the transmission range of fluorescence, reduce the loss of fluorescence signal, the utilization rate of light is improved, to improve accuracy in detection.
Description
Technical field
The utility model relates to cell analysis technical fields, and in particular to a kind of to fill for the optics of cell count and detection
It sets.
Background technique
Currently, the instrument for cell count and detection has flow cytometer, cellanalyzer, leukocyte differential count to detect
Instrument etc., it is fast that these instruments are all based on the light scattering technique progress multi-parameters such as cell volume size and cell interior complexity
Fast quantitative analysis, to realize the cell analysis instrument analyzed specific cell colony and sorted.These cell analysis instruments
Working principle be: laser light source by multiple lens carry out shaping focusing, compression spot size to certain size range in,
And expose to laser beam on the pore in sheath flow device by reflecting mirror, when laser beam is radiated at capillary by sheath flow device
Scattered light signal can be generated when on the cell in hole, be then collected by lens on light signal, focussed collimated propagates to light
Electric multiplier tube or photodiode carry out the reception and conversion of optical signal, and electric signal sent into host computer carry out it is a series of
Signal processing realizes the signature analysis to cell.When analyzing due to host computer cell, it is all based on cell scattering light letter
It number realizes, therefore the light path system of cell analysis instrument determines the performance of cell analysis instrument, light path system is designed
More reasonable, the loss of cell scattered light signal is smaller, and the signal-to-noise ratio of cell scattered light signal is higher, the detection knot of cell analysis instrument
Fruit is then more accurate.
Utility model content
The utility model is intended to provide a kind of Optical devices for cell count and detection, can reduce cell scattering
The loss of optical signal improves the signal-to-noise ratio of scattered light signal, realizes the accurate detection of eucaryotic cell structure and bulk properties.
To solve the above problems, the utility model is achieved through the following technical solutions:
A kind of Optical devices for cell count and detection, mainly by pedestal, and the light source being mounted on the base is whole
Shape unit, sheath flow device, 0 degree and 10 degree of signal receiving units, light element, fluorescence signal receiving unit composition；Sheath flow device is located at
On the optical path joint of light source shaping unit, fluorescence signal receiving unit and 0 degree and 10 degree of signal receiving unit this 3 units；
Wherein the incidence end of the exit end of light source shaping unit and sheath flow device connects, one of exit end of sheath flow device and 0 degree and 10
The incidence end connection of signal receiving unit is spent, another exit end of sheath flow device and the incidence end of light element connect, guide-lighting single
The exit end of member is connect with the incidence end of fluorescence signal receiving unit.
In above scheme, fluorescence signal receiving unit receives shell by signal, and is located at signal and receives intracorporal 5, shell
Fluorescence dichroscope and 6 photoelectric converter compositions；The incidence end of first fluorescence dichroscope forms fluorescence signal receiving unit
Incidence end；The reflection output end of first fluorescence dichroscope is connect with the first photoelectric converter；First fluorescence dichroscope
Transmission output end is connect with the incidence end of the second fluorescence dichroscope；The reflection output end of second fluorescence dichroscope and the 5th glimmering
The incidence end of light dichroscope connects, the incidence end of the transmission output end and third fluorescence dichroscope of the second fluorescence dichroscope
Connection；The reflection output end of 5th fluorescence dichroscope is connect with the 6th photoelectric converter；The transmission of 5th fluorescence dichroscope
Output end is connect with the 5th photoelectric converter；The incident output end of third fluorescence dichroscope connects the 4th fluorescence dichroscope
Incidence end；The transmission output end of third fluorescence dichroscope is connect with the second photoelectric converter；4th fluorescence dichroscope it is anti-
Output end is penetrated to connect with third photoelectric converter；The transmission output end of 4th fluorescence dichroscope and the 4th photoelectric converter connect
It connects.
As an improvement, the output end of fluorescence dichroscope is connect by bandpass filter with photoelectric converter.
It is received in shell as an improvement, fluorescence dichroscope is movably mounted to signal using plug type.
In above scheme, light element is by guide-lighting pedestal, preceding guide-lighting meniscus lens, rear guide-lighting meniscus lens, cemented doublet, aobvious
The micro- eyepiece peace of micro- lens barrel, slit baffle, plano-concave highlights micro- eyepiece composition；Guide-lighting through-hole is offered in guide-lighting pedestal；
The entrance of guide-lighting through-hole forms the incidence end of light element, and towards sheath flow device；The outlet of guide-lighting through-hole forms light element
Exit end, and towards fluorescence signal receiving unit；Preceding leaded light meniscus lens, rear guide-lighting meniscus lens, cemented doublet and microcobjective
Mirror is sequentially arranged in the inlet of guide-lighting through-hole from front to back, and preceding guide-lighting meniscus lens are positioned close to the entrance one of guide-lighting through-hole
Side, microcobjective mirror are positioned close to the outlet side of guide-lighting through-hole；The micro- eyepiece peace of slit baffle, plano-concave highlights micro- eyepiece
It is sequentially arranged in the exit of guide-lighting through-hole from front to back, and slit baffle is positioned close to the inlet side of guide-lighting through-hole, puts down
Highlight the outlet side that micro- eyepiece is positioned close to guide-lighting through-hole.
In above scheme, light element is fiber optic bundle, and one end of the fiber optic bundle is coupled with sheath flow device, and the other end and fluorescence are believed
The incidence end coupling of number receiving unit.
As an improvement, having additional a plano-convex lens between fiber optic bundle and sheath flow device.
In above scheme, 0 degree and 10 degree of signal receiving units receive pedestal, diaphragm, forward direction condenser, perforation by first
Mirror, 0 degree of signal receiving circuit plate and 10 degree of signal receiving circuit board groups at；First reception pedestal in offer be connected enter light
Through-hole, 0 degree of light through-hole out of light through-hole and 10 degree out；0 degree out light through-hole coincide with the central axes for entering light through-hole, 10 degree out light it is logical
Hole and the central axes for entering light through-hole are perpendicular, and the central axes intersection for entering light through-hole, 0 degree of light through-hole out of light through-hole and 10 degree out exists
The intersection of this 3 through-holes；Perforated mirror is arranged in the intersection, and enter the outlet of light through-hole by perforated mirror with 0 degree out light lead to
The entrance of the light through-hole out of the entrance in hole and 10 degree is connected；The entrance for entering light through-hole forms entering for 0 degree and 10 degree signal receiving unit
End is penetrated, and towards sheath flow device, and the inlet into light through-hole is arranged in forward direction condenser；0 degree of signal receiving circuit plate is arranged 0
Spend the exit of light through-hole out, the exit of 10 degree of light through-holes out is arranged in 10 degree of signal receiving circuit plates.
As an improvement, the front side of forward direction condenser is equipped with diaphragm.
In above scheme, light source shaping unit is mainly by 2 lasers, 4 cylindrical mirrors, 1 reflecting mirror, 1 light source two
To Look mirror and 1 light-resource fousing lens composition；2 lasers have different wavelength, and one of laser is mutual by 2
It is incident on reflecting mirror after orthogonal cylindrical mirror, and is incident on after the reflection of reflecting mirror one of them of light source dichroscope and enters
Penetrate end；Another laser, which then passes through, to be directly incident on another of light source dichroscope after 2 mutually orthogonal cylindrical mirrors and enters
Penetrate end；The incidence end of the exit end connection light-resource fousing lens of light source dichroscope, the exit end of light-resource fousing lens form light
The exit end of source shaping unit.
As an improvement, sheath flow device can be realized the orientation adjustable plate for realizing the adjusting of X-axis, Y-axis and Z-direction by one
It is mounted on the base.
Compared with prior art, the utility model has the following characteristics:
1, it is designed by the Optical devices to cell analysis instrument, the utilization efficiency and fluorescence for improving exciting light pass
Efficiency is passed, the transmission range of fluorescence is shortened, reduces the loss of fluorescence signal, improves the utilization rate of light, to improve
Accuracy in detection.
2, it is shone using bidifly and carries out the detection of cell, the multifrequency nature of a cell can analyzed, improve detection
Efficiency；
3, the fluorescence dichroscope of fluorescence signal receiving unit is designed using plug type, convenient for multiple fluorescence channels from
By replacing, convenient for channel configuration change and flexible upgrading, use cost and maintenance cost greatly have been saved for user；
4, light element is scattered the collection of fluorescence signal using light transmission type optical fiber, can reduce optical path to a certain extent
Structure, so that equipment instrument minimizes, cost is more cheaper, and the low damage of optical signal is realized using optical fiber total reflection characteristic
Consumption.
Detailed description of the invention
Fig. 1 is a kind of schematic perspective view of Optical devices for cell count and detection；
Fig. 2 is a kind of top view of Optical devices for cell count and detection；
Fig. 3 is a kind of light path principle figure of Optical devices for cell count and detection；
Fig. 4 is the schematic perspective view of light source shaping unit；
Fig. 5 is the sectional axonometric drawing of 0 degree and 10 degree signal receiving unit；
Fig. 6 is a kind of sectional axonometric drawing (embodiment one) of light element；
Fig. 7 is the sectional view of fluorescence signal receiving unit (removing photoelectric converter)；
Fig. 8 is the light path principle figure of fluorescence signal receiving unit；
Fig. 9 is the scheme of installation (embodiment two) of another light element；
Figure label: 1, pedestal；2, light source shaping unit, 2-1 laser, 2-2, cylindrical mirror, 2-3, reflecting mirror, 2-4, light
Source dichroscope, 2-5, light-resource fousing lens；3, sheath flow device；4,4-1,0 degree and 10 degree of signal receiving units receive pedestal, 4-
1-1, enter light through-hole, 4-1-2,0 degree of light through-hole out, 4-1-3,10 degree of light through-holes out, 4-2, diaphragm, 4-3, forward direction condenser, 4-
4, perforated mirror, 4-5,0 degree of signal receiving circuit plate, 4-6,10 degree of signal receiving circuit plates；5, light element, 5-1, guide-lighting base
Seat, 5-1-1, guide-lighting through-hole, 5-2, preceding guide-lighting meniscus lens, 5-3, rear guide-lighting meniscus lens, 5-4, cemented doublet, 5-5, micro- object
Mirror lens barrel, 5-6, microcobjective regulating part, 5-7, slit baffle, the micro- eyepiece of 5-8, plano-concave, the micro- eyepiece of 5-9, plano-convex, 5-
10, fiber optic bundle；6, fluorescence signal receiving unit, 6-1, signal receive shell, 6-2, fluorescence dichroscope, 6-3, bandpass filter
Piece, 6-4, photoelectric converter.
Specific embodiment
For the purpose of this utility model, technical solution and advantage is more clearly understood, below in conjunction with specific example, and join
According to attached drawing, the utility model is further described.It should be noted that the direction term mentioned in example, such as "upper",
"lower", " in ", " left side " " right side ", "front", "rear" etc., be only the direction with reference to attached drawing.Therefore, the direction used is intended merely to illustrate
Not it is used to limit the protection scope of the utility model.
Embodiment one:
Referring to Fig. 1-3, a kind of Optical devices for cell count and detection mainly by pedestal 1, and are mounted on pedestal
3,0 degree of light source shaping unit 2, sheath flow device and 10 degree of signal receiving units 4, light element 5, fluorescence signal receiving unit 6 on 1
Composition.Sheath flow device 3 is located at light source shaping unit 2, fluorescence signal receiving unit 6 and 0 degree and 10 degree of signal receiving units 4 this 3
On the optical path joint of unit.Wherein the exit end of light source shaping unit 2 is connect with the incidence end of sheath flow device 3, sheath flow device 3 its
In an exit end connect with the incidence end of 0 degree and 10 degree signal receiving unit 4, another exit end of sheath flow device 3 and guide-lighting
The incidence end of unit 5 is connected with exit end, and the exit end of light element 5 is connect with the incidence end of fluorescence signal receiving unit 6.Light
The laser beam of the output of source shaping unit 2 shines directly on the pore in sheath flow device 3.Laser beam is passing through sheath flow device
3 when being radiated on the cell in pore, generates scattered light signal.A part in the scattered light signal enters directly into 0 degree
And in 10 degree of signal receiving units 4, another part in scattered light signal enters fluorescence signal receiving unit by light element 5
6.0 degree and 10 degree of signal receiving units 4 and fluorescence signal receiving unit 6 send into host computer after being collected to optical signal and carry out
Cell analysis.
Above-mentioned light source shaping unit 2 is mainly by 2 laser 2-1,4 cylindrical mirror 2-2,1 reflecting mirror 2-3,1 light
Source dichroscope 2-4 and 1 light-resource fousing lens 2-5 composition.2 laser 2-1 have different wavelength.In the present embodiment
In, one of laser 2-1 is the red laser 2-1 of 635nm, another laser 2-1 is the blue laser of 488nm
Device 2-1.One of laser 2-1 passes through reflection by being incident on reflecting mirror 2-3 after 2 mutually orthogonal cylindrical mirror 2-2
One of incidence end of light source dichroscope 2-4 is incident on after the reflection of mirror 2-3.Another laser 2-1 is mutual by 2
Another incidence end of light source dichroscope 2-4 is directly incident on after orthogonal cylindrical mirror 2-2.Light source dichroscope 2-4's goes out
End is penetrated to be incident in the pore of sheath flow device 3 via light-resource fousing lens 2-5.The round laser that one laser 2-1 launches
For hot spot after 2 mutually orthogonal cylindrical mirror 2-2, circular light spot will be compressed into one by cylindrical mirror 2-2 has specific ruler
Very little elliptical spot, the subsequent elliptical spot are radiated on reflecting mirror 2-3, and are passed through light source after reflecting mirror 2-3 reflection
Dichroscope 2-4 is focused on sheath flow device 3 finally by light-resource fousing lens 2-5, carries out detection of particles.And at the same time, separately
For the round laser facula that one laser 2-1 launches after 2 mutually orthogonal cylindrical mirror 2-2, circular light spot will be by
Cylindrical mirror 2-2 is compressed into the elliptical spot with specific dimensions, the subsequent elliptical spot directly through light source two to
Look mirror 2-4, and focused on sheath flow device 3 by light-resource fousing lens 2-5, carry out detection of particles.For spot shaping cylindrical mirror
2-2 can be other than laser 2-1 light-emitting window, while shaping cylindrical mirror 2-2 is also mountable within laser 2-1, that is, is integrated
Formula shaped laser device 2-1.Referring to fig. 4.
There is cell to be measured in the pore of above-mentioned sheath flow device 3.Sheath flow device 3 is mounted on pedestal 1 by an orientation adjustable plate
On, which is able to drive the movement that sheath flow device 3 realizes X-axis, Y-axis and Z-direction, to guarantee the capillary of sheath flow device 3
Hole is located exactly at light source shaping unit 2, fluorescence signal receiving unit 6 and 0 degree and 10 degree of signal receiving unit 4 this 3 units
On optical path joint.The elliptical spot with specific dimensions that light source shaping unit 2 is emitted is radiated to be checked in sheath flow device 3
When particle, light beam will generate 360 ° of light scattering along particle, and be believed respectively by fluorescence signal receiving unit 6 and 0 degree and 10 degree
Number receiving unit 4 receives.
Above-mentioned 0 degree and 10 degree of signal receiving units 4 are by reception pedestal 4-1, diaphragm 4-2, forward direction condenser 4-3, perforated mirror
4-4,0 degree of signal receiving circuit plate 4-5 and 10 degree of signal receiving circuit plate 4-6 compositions.It receives to offer in pedestal 4-1 and be connected
Enter light through hole socket 4-1-1,0 degree of light through-hole 4-1-3 out of light through-hole 4-1-2 and 10 degree out.0 degree light through-hole 4-1-2 and enters light out
The central axes of through hole socket 4-1-1 coincide, 10 degree out light through-hole 4-1-3 and the central axes for entering light through hole socket 4-1-1 it is perpendicular,
And enter light through hole socket 4-1-1, the central axes of 0 degree of light through-hole 4-1-3 out of light through-hole 4-1-2 and 10 degree out are intersected in this 3 through-holes
Intersection.Perforated mirror 4-4 is arranged in the intersection.Enter the outlet of light through hole socket 4-1-1 and passes through the light out of perforated mirror 4-4 and 0 degree
The entrance of the light through-hole 4-1-3 out of the entrance of through-hole 4-1-2 and 10 degree is connected.Enter the entrance of light through hole socket 4-1-1 towards sheath flow device
3.The inlet into light through hole socket 4-1-1 is arranged in forward direction condenser 4-3.Diaphragm 4-2 be placed in front of to the front side of condenser 4-3,
Its main function is to block the light beam that diffraction, scattering phenomenon does not occur with particle, thus promotes the reliability of detection, and reduce letter
The background voltage of number circuit board for receiving.The exit of 0 degree of light through-hole 4-1-2 out is arranged in 0 degree of signal receiving circuit plate 4-5. 10
The exit of 10 degree of light through-hole 4-1-3 out is arranged in degree signal receiving circuit plate 4-6.When 3 scattered beam of sheath flow device by it is preceding to
When condenser 4-3, scattered beam will gradually be assembled, and a part of low-angle light will be by perforated mirror 4-4, and passes through 0 degree
Light through-hole 4-1-2 is propagated on 0 degree of signal receiving circuit plate 4-5 out, and the light of another part wide-angle will be by perforated mirror
4-4 reflection, and by 10 degree out light through-hole 4-1-3 propagate on 10 degree of signal receiving circuit plate 4-6, this completes optical signals
Signal in 0 degree and 10 degree direction receives.Referring to Fig. 5.
It, will when on the cell particles specifically bound with fluorescent particle when laser beam is radiated in sheath flow device 3
It will do it fluorescence excitation and generate Stokes frequency domain, cause light beam wavelength to infrared direction transition.Therefore with light beam advance side
Fluorescence signal is imported into fluorescence signal receiving unit 6 to one light element 5 of setting.In the present embodiment, light element 5
Using micro- collimation lens set, mainly by guide-lighting pedestal 5-1, preceding guide-lighting meniscus lens 5-2, rear guide-lighting meniscus lens 5-3, double gluings
Lens 5-4, microcobjective lens barrel 5-5, slit baffle 5-7, the micro- eyepiece 5-8 peace of plano-concave highlight micro- eyepiece 5-9 composition.It is guide-lighting
Guide-lighting through-hole 5-1-1 is offered in pedestal 5-1.Towards sheath flow device 3, guide-lighting through-hole 5-1-1's goes out the entrance of guide-lighting through-hole 5-1-1
Mouthful towards fluorescence signal receiving unit 6.Preceding leaded light meniscus lens 5-2, rear guide-lighting meniscus lens 5-3, cemented doublet 5-4 and micro- object
Mirror mirror is sequentially arranged in the inlet of guide-lighting through-hole 5-1-1 from front to back, and preceding leaded light meniscus lens 5-2 is positioned close to leaded light
The inlet side of through-hole 5-1-1, microcobjective mirror are positioned close to the outlet side of guide-lighting through-hole 5-1-1.Slit baffle 5-7,
The micro- eyepiece 5-8 peace of plano-concave highlights the exit that micro- eyepiece 5-9 is sequentially arranged in guide-lighting through-hole 5-1-1 from front to back, and narrow
Seam baffle 5-7 is positioned close to the inlet side of guide-lighting through-hole 5-1-1, and the micro- eyepiece 5-9 of plano-convex is positioned close to guide-lighting through-hole
The outlet side of 5-1-1.It is 1~3 degree and 7~11 degree two that the effect of slit baffle 5-7, which is by forward-scattering signal beam splitting,
Light component.On microcobjective lens barrel 5-5 have microcobjective regulating part 5-6, to the focus to microcobjective lens barrel 5-5 into
Row is adjusted.The focal length of micro-imaging lens group is 35~100mm.The fluorescence signal that sheath flow device 3 scatters is first successively by leading
Preceding guide-lighting meniscus lens 5-2, rear guide-lighting meniscus lens 5-3, cemented doublet 5-4 and the microcobjective lens barrel 5- of 5 inlet of light unit
5, then in turn through the slit baffle 5-7 of 5 inlet of light element, the micro- eyepiece 5-8 peace of plano-concave highlights micro- eyepiece 5-9,
Last collimation focusing is in the fluorescence signal receiving unit 6 of rear end.Referring to Fig. 6.
Above-mentioned fluorescence signal receiving unit 6 receives shell 6-1 by signal, and 5 be located in signal reception shell 6-1
A fluorescence dichroscope 6-2,6 bandpass filter 6-3 and 6 photoelectric converter 6-4 compositions.All fluorescence dichroscope 6-2
Signal is movably mounted to using plug type to receive in shell 6-1.All fluorescence dichroscope 6-2 and fluorescent scattering signal master
The angular range of light is 25~50 °.Signal, which receives, is equipped with extinction cotton in shell 6-1, can be anti-by fluorescence dichroscope 6-2
Remaining wavelength will be irradiated on extinction cotton after penetrating and transmiting, and be avoided unnecessary photosignal and be generated.5 fluorescence two to
Look mirror 6-2 has different transmission peak wavelengths, and fluorescence dichroscope 6-2 is mounted on signal at 45 degree of angles and receives in shell 6-1,
Main function is to separate the light beam of different wave length, by reflecting back along 90 ° of directions for specific wavelength, and is allowed specific
Wavelength penetrates.When light has stepped through fluorescence dichroscope 6-2, light intensity will gradually weaken, while entrained particle
Information will gradually reduce.The incidence end of first fluorescence dichroscope 6-2 forms the incidence end of fluorescence signal receiving unit 6.5 bands
Pass filter 6-3 has different filter wavelengths, and main function is that non-specific wavelength is stopped to pass through, and only allows specific wavelength logical
It crosses, thus plays the role of clean signal.Bandpass filter 6-3 is being received in shell using that can intercut formula case.Photoelectric conversion
Device 6-4 main function is to convert optical signals into electric signal output, and be sent to host computer and show particle entrained by it
Characteristic.In the present embodiment, photoelectric converter 6-4 can be photomultiplier tube, avalanche photodide, photodiode or its
His charge coupled cell.
The reflection output end of first fluorescence dichroscope 6-2 is via the first bandpass filter 6-3 and the first photoelectric converter
6-4 connection, the fluorescent scattering signal chief ray after being excited are incident on the first fluorescence dichroscope 6-2 with 45° angle.First is glimmering
The transmission output end of light dichroscope 6-2 is connect with the incidence end of the second fluorescence dichroscope 6-2.Second fluorescence dichroscope
The reflection output end of 6-2 is connect with the incidence end of the 5th fluorescence dichroscope 6-2, and the transmission of the second fluorescence dichroscope 6-2 is defeated
Outlet is connect with the incidence end of third fluorescence dichroscope 6-2.The reflection output end of 5th fluorescence dichroscope 6-2 is via
Six bandpass filter 6-3 are connect with the 6th photoelectric converter 6-4.The transmission output end of 5th fluorescence dichroscope 6-2 is via
Five bandpass filter 6-3 are connect with the 5th photoelectric converter 6-4.The incident output end connection the of third fluorescence dichroscope 6-2
The incidence end of four fluorescence dichroscope 6-2.The transmission output end of third fluorescence dichroscope 6-2 is via the second bandpass filter 6-
3 connect with the second photoelectric converter 6-4.The reflection output end of 4th fluorescence dichroscope 6-2 is via third bandpass filter 6-
3 connect with third photoelectric converter 6-4.The transmission output end of 4th fluorescence dichroscope 6-2 is via the 4th bandpass filter 6-3
It is connect with the 4th photoelectric converter 6-4.Referring to Fig. 7 and 8.
Embodiment two:
Embodiment two and the structure of the Optical devices of embodiment one are substantially the same, difference be light element 5 using
One end of fiber optic bundle 5-10, rather than micro- collimation lens set, fiber optic bundle 5-10 are coupled with sheath flow device 3, and the other end and fluorescence are believed
The incidence end coupling of number receiving unit 6.Referring to Fig. 9.It can be realized micro- damage of optical signal using Y type light transmission type fiber optic bundle 5-10
Consumption, general standard single mode optical fiber are 0.2dB/Km in the loss factor of 1550nm.Fiber-optic signal receiving end and sheath flow device 3 away from
From for 2~10mm.In addition, also having additional the plano-convex lens of a large-numerical aperture, plano-convex between fiber optic bundle 5-10 and sheath flow device 3
The main function of lens is to collect light beam, preferably enters light beam coupling in optical fiber.
It should be noted that although the above embodiment described in the utility model be it is illustrative, this is not to this
The limitation of utility model, therefore the utility model is not limited in above-mentioned specific embodiment.The utility model is not being departed from
In the case where principle, the other embodiment that all those skilled in the art obtain under the enlightenment of the utility model is accordingly to be regarded as
Within the protection of the utility model.
Claims (10)
1. a kind of Optical devices for cell count and detection, characterized in that mainly by pedestal (1), and be mounted on pedestal
(1) light source shaping unit (2), sheath flow device (3), 0 degree and 10 degree of signal receiving units (4), light element (5) and fluorescence letter on
Number receiving unit (6) composition；
Sheath flow device (3) is located at light source shaping unit (2), fluorescence signal receiving unit (6) and 0 degree and 10 degree of signal receiving units
(4) on the optical path joint of this 3 units；Wherein the incidence end of the exit end of light source shaping unit (2) and sheath flow device (3) connects
It connects, one of exit end of sheath flow device (3) is connect with the incidence end of 0 degree and 10 degree signal receiving unit (4), sheath flow device (3)
Another exit end connect with the incidence end of light element (5), the exit end of light element (5) and fluorescence signal receiving unit
(6) incidence end connection.
2. a kind of Optical devices for cell count and detection according to claim 1, characterized in that fluorescence signal connects
It receives unit (6) and shell (6-1) is received by signal, and 5 fluorescence dichroscope (6- being located in signal reception shell (6-1)
2) it is formed with 6 photoelectric converters (6-4)；
The incidence end of first fluorescence dichroscope (6-2) forms the incidence end of fluorescence signal receiving unit (6)；First fluorescence two to
The reflection output end of Look mirror (6-2) is connect with the first photoelectric converter (6-4)；The transmission of first fluorescence dichroscope (6-2) is defeated
Outlet is connect with the incidence end of the second fluorescence dichroscope (6-2)；The reflection output end of second fluorescence dichroscope (6-2) and
The incidence end of five fluorescence dichroscopes (6-2) connects, the transmission output end and third fluorescence two of the second fluorescence dichroscope (6-2)
It is connected to the incidence end of Look mirror (6-2)；The reflection output end and the 6th photoelectric converter (6- of 5th fluorescence dichroscope (6-2)
4) it connects；The transmission output end of 5th fluorescence dichroscope (6-2) is connect with the 5th photoelectric converter (6-4)；Third fluorescence two
The incidence end of the 4th fluorescence dichroscope (6-2) is connected to the incident output end of Look mirror (6-2)；Third fluorescence dichroscope (6-
2) transmission output end is connect with the second photoelectric converter (6-4)；The reflection output end of 4th fluorescence dichroscope (6-2) and
Three photoelectric converters (6-4) connection；The transmission output end and the 4th photoelectric converter (6-4) of 4th fluorescence dichroscope (6-2)
Connection.
3. a kind of Optical devices for cell count and detection according to claim 2, characterized in that fluorescence dichroic
The output end of mirror (6-2) is connect by bandpass filter (6-3) with photoelectric converter (6-4).
4. a kind of Optical devices for cell count and detection according to claim 2, characterized in that fluorescence dichroic
Mirror (6-2) is movably mounted to signal using plug type and receives in shell (6-1).
5. a kind of Optical devices for cell count and detection according to claim 1, characterized in that light element
(5) by guide-lighting pedestal (5-1), preceding guide-lighting meniscus lens (5-2), rear guide-lighting meniscus lens (5-3), cemented doublet (5-4), micro- object
Mirror lens barrel (5-5), slit baffle (5-7), the micro- eyepiece of plano-concave (5-8) peace highlight micro- eyepiece (5-9) composition；
Guide-lighting through-hole (5-1-1) is offered in guide-lighting pedestal (5-1)；The entrance of guide-lighting through-hole (5-1-1) forms light element (5)
Incidence end, and towards sheath flow device (3)；The outlet of guide-lighting through-hole (5-1-1) forms the exit end of light element (5), and direction
Fluorescence signal receiving unit (6)；Preceding leaded light meniscus lens (5-2), rear guide-lighting meniscus lens (5-3), cemented doublet (5-4) with it is micro-
Object lens mirror is sequentially arranged in the inlet of guide-lighting through-hole (5-1-1) from front to back, and preceding guide-lighting meniscus lens (5-2) are positioned close to
The inlet side of guide-lighting through-hole (5-1-1), microcobjective mirror are positioned close to the outlet side of guide-lighting through-hole (5-1-1)；Slit
Baffle (5-7), the micro- eyepiece of plano-concave (5-8) peace highlight micro- eyepiece (5-9) and are sequentially arranged in guide-lighting through-hole (5-1- from front to back
1) exit, and slit baffle (5-7) is positioned close to the inlet side of guide-lighting through-hole (5-1-1), the micro- eyepiece (5- of plano-convex
9) it is positioned close to the outlet side of guide-lighting through-hole (5-1-1).
6. a kind of Optical devices for cell count and detection according to claim 1, characterized in that light element
It (5) is fiber optic bundle (5-10) that one end of the fiber optic bundle (5-10) is coupled with sheath flow device (3), the other end and fluorescence signal receive list
The incidence end coupling of first (6).
7. a kind of Optical devices for cell count and detection according to claim 6, characterized in that fiber optic bundle (5-
10) plano-convex lens are had additional between sheath flow device (3).
8. a kind of Optical devices for cell count and detection according to claim 1, characterized in that 0 degree and 10 degree
Signal receiving unit (4) is by reception pedestal (4-1), diaphragm (4-2), forward direction condenser (4-3), perforated mirror (4-4), 0 degree of signal
Circuit board for receiving (4-5) and 10 degree of signal receiving circuit plate (4-6) compositions；
It receives and offers entering of being connected in pedestal (4-1) light through hole socket (4-1-1), 0 degree light through-hole (4-1-2) and 10 degree are out out
Light through-hole (4-1-3)；0 degree out light through-hole (4-1-2) coincide with the central axes for entering light through hole socket (4-1-1), 10 degree out light it is logical
Hole (4-1-3) and the central axes for entering light through hole socket (4-1-1) are perpendicular, and enter light through hole socket (4-1-1), 0 degree of light through-hole (4- out
1-2) and 10 degree out light through-hole (4-1-3) central axes intersection this 3 through-holes intersection；Perforated mirror (4-4) is arranged at this
Intersection, and enter the outlet of light through hole socket (4-1-1) and pass through the entrance and 10 of perforated mirror (4-4) and 0 degree of light through-hole (4-1-2) out
The entrance for spending light through-hole (4-1-3) out is connected；The entrance for entering light through hole socket (4-1-1) forms 0 degree and 10 degree of signal receiving units
(4) incidence end, and towards sheath flow device (3), and forward direction condenser (4-3) is arranged in the inlet for entering light through hole socket (4-1-1)；
The setting of 0 degree of signal receiving circuit plate (4-5) is in the exit of 0 degree of light through-hole (4-1-2) out, 10 degree of signal receiving circuit plate (4-
6) it is arranged in the exit of 10 degree of light through-holes (4-1-3) out.
9. a kind of Optical devices for cell count and detection according to claim 1, characterized in that light source shaping list
First (2) are mainly by 2 lasers (2-1), 4 cylindrical mirrors (2-2), 1 reflecting mirror (2-3), 1 light source dichroscope (2-4)
It is formed with 1 light-resource fousing lens (2-5)；
2 lasers (2-1) have different wavelength, and one of laser (2-1) passes through 2 mutually orthogonal cylindrical mirrors
It is incident on reflecting mirror (2-3) after (2-2), and is incident on light source dichroscope (2-4) its after the reflection of reflecting mirror (2-3)
In an incidence end；Another laser (2-1) is then by being directly incident on light source after 2 mutually orthogonal cylindrical mirrors (2-2)
Another incidence end of dichroscope (2-4)；Exit end connection light-resource fousing lens (2-5) of light source dichroscope (2-4)
Incidence end, the exit end of light-resource fousing lens (2-5) form the exit end of light source shaping unit (2).
10. a kind of Optical devices for cell count and detection according to claim 1, characterized in that sheath flow device (3)
The orientation adjustable plate that can be realized the adjusting for realizing X-axis, Y-axis and Z-direction by one is mounted on pedestal (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821356284.8U CN209086141U (en) | 2018-08-22 | 2018-08-22 | A kind of Optical devices for cell count and detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821356284.8U CN209086141U (en) | 2018-08-22 | 2018-08-22 | A kind of Optical devices for cell count and detection |
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| Publication Number | Publication Date |
|---|---|
| CN209086141U true CN209086141U (en) | 2019-07-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| Country | Link |
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| CN (1) | CN209086141U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109060749A (en) * | 2018-08-22 | 2018-12-21 | 桂林优利特医疗电子有限公司 | A kind of Optical devices for cell count and detection |
| CN115015178A (en) * | 2022-08-05 | 2022-09-06 | 天津迈科隆生物科技有限公司 | Optical detection device and blood analyzer |
-
2018
- 2018-08-22 CN CN201821356284.8U patent/CN209086141U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109060749A (en) * | 2018-08-22 | 2018-12-21 | 桂林优利特医疗电子有限公司 | A kind of Optical devices for cell count and detection |
| CN109060749B (en) * | 2018-08-22 | 2024-03-29 | 桂林优利特医疗电子有限公司 | Optical device for cell counting and detection |
| CN115015178A (en) * | 2022-08-05 | 2022-09-06 | 天津迈科隆生物科技有限公司 | Optical detection device and blood analyzer |
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