CN203249851U - Cell scattering light multi-position detection cytometer - Google Patents
Cell scattering light multi-position detection cytometer Download PDFInfo
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- CN203249851U CN203249851U CN 201320260046 CN201320260046U CN203249851U CN 203249851 U CN203249851 U CN 203249851U CN 201320260046 CN201320260046 CN 201320260046 CN 201320260046 U CN201320260046 U CN 201320260046U CN 203249851 U CN203249851 U CN 203249851U
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Abstract
The utility model relates to a cell scattering light multi-position detection cytometer, which comprises a laser radiation mechanism, a cell sheath flow mechanism, a scattering light collection mechanism and a signal analyzing and processing mechanism, wherein the scattering light collection mechanism comprises one set of forward scattering light detector and 2-4 sets of 90-degree scattering light detectors arranged at an incident part of the laser and on a plane vertical to the incident direction of the laser; the distances from the 90-degree scattering light detectors to the center line of a sheath flow pipe are equal, and the 90-degree scattering light detectors are symmetrically arranged according to the center line of the sheath flow pipe. The cell scattering light multi-position detection cytometer is additionally equipped with 90-degree scattering light detection passages, so that the signal light intensity of the obtained 90-degree scattering light is high, the signal to noise ratio is high, and the accuracy and the stability of detection are improved; the magnification times and the noise reduction function of the cytometer component can be reduced, so that the instrument cost is lowered and the reliability is improved.
Description
Technical field
The utility model belongs to technical field of biological, is specifically related to a kind of cell scattering light multiposition and detects cytoanalyze.
Background technology
Flow cytometer (flow cytometer, FCM) is a kind of cell analysis instrument according to light scattering theory.During each cell of the single arrangement of flowing through in the flow chamber when Ear Mucosa Treated by He Ne Laser Irradiation because the physical characteristics of cell, part light from the cell through different scattered through angles.Wherein, the light intensity of Small angle forward scattering light can reflect cell volume; The light intensity of large-angle scattered light can reflect the information of nucleus complexity and cell granulations; And the light intensity of side scattered light can the reacting cells film, nuclear membrane, cytoplasmic variation, thereby cell is classified, and cell DAN, RNA are analyzed.
Existing flow cytometer is by sheath stream technology cell to be lined up 1 row, pass through one by one the optical detection window of sheath flow device, the Ear Mucosa Treated by He Ne Laser Irradiation that laser instrument produces is at the optical detection window, and individual cells is subject to Ear Mucosa Treated by He Ne Laser Irradiation successively, scatters forward direction 0 degree, 10 degree scattered lights and 90 degree scattered lights.Settle a cover pick-up unit on each forward direction and the 90 degree directions, photomultiplier is converted to electric signal with scattered light intensity, for Computer Analysis.
The cell scattering trimmed book comes just not strong, also will carry out 2 times, 3 times even more frequently light splitting at 90 scattered lights of spending directions, and polarized light, depolarized light, fluorescent exciting etc. are different to be detected to carry out, and each its intensity of light splitting all weakens 50% at least.Have to can't avoid noise to strengthen with the photomultiplier of high-gain for the 90 degree scattered light information that detect cell, this strengthens the cost of flow cytometer, and the Stability and veracity of whole instrument also is difficult to further raising.
The utility model content
The purpose of this utility model is that a kind of cell scattering light multiposition of design detects cytoanalyze, becoming the multiple spot on 90 ° the scattered light plane of cell that pick-up unit is set with laser direction, to obtain at double cell scattering light, available photomultiplier than low gain, reduce cost, improve the Stability and veracity of instrument integral body.
When Ear Mucosa Treated by He Ne Laser Irradiation on individual cells, cell is on the plane that becomes 90 ° with laser direction at the scattered light of 90 ° of directions, on this plane, all can detect so 90 ° of scattered lights of cell, and on this plane, be equal to the cell scattering optical information of the cell distance place's of equating detection gained.
The cell scattering light multiposition of the utility model design detects cytoanalyze, comprise laser radiation mechanism, cellular sheath stream mechanism, scattered light collecting mechanism and signal analysis and processing mechanism, cellular sheath stream mechanism makes cell to be analyzed defiled in sheath flow liquid manage streamlined flow along its sheath stream, the laser that laser radiation mechanism produces shines successively to the cell in the sheath stream pipe, the laser incident direction is vertical with sheath stream pipe trend, the scattered light collecting mechanism comprises 1 cover forward scattering photodetector and 90 ° of light scattering detectors of 2～4 covers, the forward scattering photodetector be arranged at laser in the same way, 10 ° of positions, the place ahead, 90 ° of light scattering detectors are arranged on the laser incident place plane vertical with the laser incident direction.
Each 90 ° of light scattering detector present position equates with the distance of this sheath stream tube hub line.Can adopt identical light scattering detector module.
When used sheath stream tube section is rectangle, 90 ° of light scattering detectors on the laser incident place plane vertical with the laser incident direction be positioned at sheath stream tube wall up and down or about take sheath stream tube hub line as 2 symmetrical points, perhaps be positioned at sheath stream tube wall periphery with sheath stream tube hub line symmetrical 4 points in twos.
Preferred plan is for being positioned at 2 points take sheath stream tube hub line as symmetry about sheath stream tube wall at 90 ° of light scattering detectors on the laser incident place plane vertical with the laser incident direction, this line of 2 is horizontal line.
Compared with prior art, the advantage of the utility model cell scattering light multiposition detection cytoanalyze is: 1, on the basis of former detection light path, increased the sense channel of 90 ° of scattered lights, 90 ° of scattered light signal light intensity of acquisition are intrinsic 2～4 times; 2, because 90 ° of scattered light signal light intensity that obtain are high, and signal to noise ratio (S/N ratio) improves, the Stability and veracity of cell analysis improves; 3, need not the photomultiplier of adapted high-gain, reduced the requirement to equipment circuit enlargement factor and noise removal function, instrument cost reduces, Reliability Enhancement.
Description of drawings
Fig. 1 is that this cell scattering light multiposition detects cytoanalyze embodiment principle schematic;
Fig. 2 is that this cell scattering light multiposition detects cytoanalyze example structure synoptic diagram.
Number in the figure is:
1, laser radiation mechanism, 2,90 ° of light scattering detectors, 3, cellular sheath stream mechanism, 4, the forward scattering photodetector.
Embodiment
Further describe the utility model below in conjunction with the drawings and specific embodiments.
As shown in Figure 1, laser is advanced along X-direction, and sheath stream tube hub line is along the Y direction setting, and sheath stream tube section is rectangle, and tube wall is parallel to the XY plane before and after it, left and right sides tube wall and the laser perpendicular direction of advancing.When the individual cells that is arranged in rows during through laser, the cell that is subject to Ear Mucosa Treated by He Ne Laser Irradiation is at the scattered light of 90 ° of directions on the plane P that becomes 90 ° with laser direction, namely Fig. 1 the YZ plane.
On plane P, be equal to the cell scattering optical information of detection gained on the point that sheath stream tube hub linear distance equates.For example desirable A, the B that equates with sheath stream tube hub linear distance, C, a D4 point are check point.
This is for example shown in Figure 2, this routine cytoanalyze by upper routine cell scattering light multiposition detection method design comprises laser radiation mechanism 1, cellular sheath stream mechanism 3, scattered light collecting mechanism and signal analysis and processing mechanism, and the laser that laser radiation mechanism 1 produces is advanced along X-axis; Cellular sheath stream mechanism 3 makes cell to be analyzed, and defiled is along its sheath stream pipe streamlined flow in sheath flow liquid, and sheath stream pipe is along the Y direction setting, and sheath stream tube section is rectangle, and tube wall is parallel to the XY plane before and after it, left and right sides tube wall and the laser perpendicular direction of advancing.The intersection point of laser and sheath stream pipe is true origin.The laser that laser radiation mechanism 1 produces shines successively to the cell of the process true origin in the sheath stream pipe.
The scattered light collecting mechanism comprises three cover light scattering detectors.Through the 10 ° of positions, the place ahead after the true origin, namely the F of Fig. 2 point is provided with forward scattering photodetector 4 at X-axis laser.At Z-direction, A, B point in about sheath stream pipe 90 ° of light scattering detectors 2 of a cover are set respectively, A, B point and initial point are apart from equating.A, B point is on Z axis, take true origin as symmetrical.The line of this routine AB is horizontal line.
Above-described embodiment only is the specific case that the purpose of this utility model, technical scheme and beneficial effect are further described, and the utility model is not to be defined in this.All any modifications of within scope of disclosure of the present utility model, making, be equal to replacement, improvement etc., all be included within the protection domain of the present utility model.
Claims (4)
1. cell scattering light multiposition detects cytoanalyze, comprise laser radiation mechanism (1), cellular sheath stream mechanism (3), scattered light collecting mechanism and signal analysis and processing mechanism, cellular sheath stream mechanism (3) makes cell to be analyzed defiled in sheath flow liquid manage streamlined flow along its sheath stream, the laser that laser radiation mechanism (1) produces shines successively to the cell in the sheath stream pipe, the laser incident direction is vertical with sheath stream pipe trend, the scattered light collecting mechanism comprises 1 cover forward scattering photodetector (4), it is characterized in that:
Also have 2～4 cover 90 ° of light scattering detectors (2), 90 ° of light scattering detectors (2) are arranged on the laser incident place plane vertical with the laser incident direction.
2. cell scattering light multiposition according to claim 1 detects cytoanalyze, it is characterized in that:
Described each 90 ° of light scattering detectors (2) present position equates with the distance of sheath stream tube hub line.
3. cell scattering light multiposition according to claim 2 detects cytoanalyze, it is characterized in that:
Described sheath stream tube section is rectangle, the 90 ° of light scattering detectors (2) on the vertical plane of laser incident place and sheath stream pipe be positioned at sheath stream tube wall up and down or about take sheath stream tube hub line as 2 symmetrical points, perhaps be positioned at sheath stream tube wall periphery with sheath stream tube hub line symmetrical 4 points in twos.
4. cell scattering light multiposition according to claim 3 detects cytoanalyze, it is characterized in that:
Be positioned at 2 symmetric points about sheath stream tube wall at the 90 ° of light scattering detectors (2) on the laser incident place plane vertical with the laser incident direction, the line of 2 symmetric points is horizontal line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320260046 CN203249851U (en) | 2013-05-14 | 2013-05-14 | Cell scattering light multi-position detection cytometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320260046 CN203249851U (en) | 2013-05-14 | 2013-05-14 | Cell scattering light multi-position detection cytometer |
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| Publication Number | Publication Date |
|---|---|
| CN203249851U true CN203249851U (en) | 2013-10-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201320260046 Expired - Lifetime CN203249851U (en) | 2013-05-14 | 2013-05-14 | Cell scattering light multi-position detection cytometer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278440A (en) * | 2013-05-14 | 2013-09-04 | 桂林优利特医疗电子有限公司 | Cell scattered light multi-position detecting method as well as cell analyzer |
| CN109060749A (en) * | 2018-08-22 | 2018-12-21 | 桂林优利特医疗电子有限公司 | A kind of Optical devices for cell count and detection |
| CN111504869A (en) * | 2020-05-15 | 2020-08-07 | 中国计量科学研究院 | Flow type aggregate impurity analyzer |
-
2013
- 2013-05-14 CN CN 201320260046 patent/CN203249851U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278440A (en) * | 2013-05-14 | 2013-09-04 | 桂林优利特医疗电子有限公司 | Cell scattered light multi-position detecting method as well as cell analyzer |
| CN109060749A (en) * | 2018-08-22 | 2018-12-21 | 桂林优利特医疗电子有限公司 | A kind of Optical devices for cell count and detection |
| CN109060749B (en) * | 2018-08-22 | 2024-03-29 | 桂林优利特医疗电子有限公司 | Optical device for cell counting and detection |
| CN111504869A (en) * | 2020-05-15 | 2020-08-07 | 中国计量科学研究院 | Flow type aggregate impurity analyzer |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131023 |
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| CX01 | Expiry of patent term |