CN110530782B - Optical system and method for eliminating side lobe signal interference - Google Patents
Optical system and method for eliminating side lobe signal interference Download PDFInfo
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- CN110530782B CN110530782B CN201910914391.0A CN201910914391A CN110530782B CN 110530782 B CN110530782 B CN 110530782B CN 201910914391 A CN201910914391 A CN 201910914391A CN 110530782 B CN110530782 B CN 110530782B
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- 210000004027 cell Anatomy 0.000 description 62
- 210000003743 erythrocyte Anatomy 0.000 description 13
- 238000010586 diagram Methods 0.000 description 11
- 230000004075 alteration Effects 0.000 description 5
- 210000000601 blood cell Anatomy 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
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- 238000002356 laser light scattering Methods 0.000 description 1
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Abstract
The invention discloses an optical system and a method for eliminating side lobe signal interference, wherein the optical system comprises a sheath flow cell unit, a signal receiving and processing unit and an illumination unit, wherein the sheath flow cell unit is used for carrying a cell sample in sheath flow so as to enable the cell sample to pass through an optical detection area one by one, the illumination unit is used for illuminating the cell sample, and the signal receiving and processing unit is used for collecting optical signals of the cell sample and converting the optical signals into electric signals; the illumination unit comprises a light source, a collimation module, a beam shaping module and an optical filter, wherein the light source is used for emitting laser, the collimation module is used for collimating the laser and forming parallel light, the beam shaping module is used for compressing and converging the parallel light, and the optical filter is used for filtering the compressed and converged light beam; the optical filter comprises an optical filter body and at least one light blocking device, wherein the optical filter body is provided with a light passing hole, each light blocking device comprises a light blocking sheet, and the light blocking sheets can move to block the edge of the light passing hole.
Description
Technical Field
The invention relates to the technical field of blood cell analysis, in particular to an optical system and method for eliminating side lobe signal interference.
Background
In clinical diagnosis and detection work, rapid and accurate platelet count is of great value. Blood cell analyzers are the main way to count platelets in clinical laboratory at all levels, and there are two main methods for blood cell analysis and platelet detection: electrical impedance methods (PLT-I) and laser scattering methods (PLT-O). Among them, the laser scattering method (PLT-O) detects blood cells by making the cells pass through a detection area, where the cells are illuminated by irradiation light, and emit scattered light or fluorescence to the whole airspace, collect useful light information, perform photoelectric conversion, then perform data processing, form a scatter diagram, and analyze an analysis object according to the scatter diagram.
As shown in fig. 1, the above-described optical system generally includes an illumination unit, a sheath flow cell unit 4, and a signal receiving processing unit 5. The lighting unit generally comprises a light source 1, a collimation module 2 and a beam shaping module 3, wherein light emitted by the light source 1 is collected by the collimation module 2 and shaped into parallel light, the parallel light is continuously compressed and shaped by the beam shaping module 3, finally an elliptic light spot is formed, and the elliptic light spot is irradiated to the sheath flow cell unit 4. The sheath flow cell unit 4 provides an optical detection region in which the cell sample is entrained in the sheath flow using the sheath flow principle, allowing the cells to pass through the region one by one, and after the cells passing through the region are illuminated by the illumination unit, scattered light or fluorescence is emitted in the full airspace. The signal receiving processing unit 5 collects scattered light and fluorescence information according to the design and converts into an electric signal. The electric signals can form an intuitive one-dimensional histogram or a two-dimensional or even three-dimensional scatter diagram after being screened, processed and analyzed, so that the classification and counting information of cells are obtained.
The light source is typically a semiconductor laser diode, which is characterized by: (1) The divergence angle perpendicular to the junction plane is large, typically between 40 ° and 60 °; (2) longitudinal astigmatism; (3) an unstable fundamental transverse mode occurs. A lens with a numerical aperture larger than 0.65 is used when the divergence angle perpendicular to the junction plane is larger than 40 degrees, and for a general aspheric collimator lens, the numerical aperture of the lens is 0.65 and is close to the design and processing limit, so that the coupling collimation effect perpendicular to the junction plane direction is reduced, the light rays at the edge of the aspheric collimator lens cannot be well coupled and collimated, larger aberration exists, and after being shaped by a cylindrical lens, a minor spot peak 7 with a small peak value is formed near the two sides of a major peak 6 of a focused spot, as shown in fig. 2. When the red blood cells pass through the focusing light spot, red blood cell sidelobe signals are correspondingly generated near the two sides of the red blood cell main lobe signal, as shown in fig. 3. Also, the presence of two factors, longitudinal astigmatism and unstable fundamental transverse mode, of the semiconductor laser diode increases the aberration of the optical system, resulting in the generation of side lobe signals.
In general, platelets and erythrocytes are detected in the same detection area, and the particle size of the platelets is much smaller than that of erythrocytes, so that the forward scattered light intensity peak value generated when the platelets pass through the irradiation area of a sheath flow focusing light spot is much smaller than that of erythrocytes, and the peak value, pulse width of side lobe signals at two sides of a main lobe signal of a erythrocyte are very similar to those of the main lobe signal of the platelets, so that an algorithm can misidentify the side lobe signal of the erythrocytes as the platelets in threshold identification, as shown in fig. 4, and the counting result of the platelets by a laser scattering method (PLT-O) is higher than that of the platelets by an electrical impedance method (PLT-I).
In order to eliminate the interference of the side lobe signal, a method is adopted, which comprises the following steps:
(1) The numerical aperture of the aspherical collimating lens is improved, the coupling collimating efficiency is optimized, the aberration is reduced, and the method has high requirements on the processing precision of the collimating lens and high manufacturing cost, as disclosed in the patent application number CN 200610062982.2;
(2) The influence caused by a large divergence angle and a high-order transverse mode is reduced by adopting an optical fiber coupling means, so that the influence of a side lobe is eliminated, for example, an utility model patent disclosed by patent application number CN201620912521.9 is an optical system for a particle analyzer, and the method needs to adopt a single-mode optical fiber for transmission and is complex to implement;
(3) The pulse is identified by an algorithm during signal processing, and small pulse signals smaller than a threshold line are removed, so that the side lobe signals accompanying with the main lobe signals of red blood cells are removed, however, the peak value and the pulse width of the main lobe signals of most of platelets (PLT-O) are similar to those of the side lobe pulse signals of red blood cells, and therefore, the side lobe signals of the red blood cells are removed, and meanwhile, the main lobe signals of useful platelets (PLT-O) are removed together, so that signal distortion is caused, and the error of cell analysis is increased.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides an optical system and a method for eliminating side lobe signal interference, which can effectively eliminate the interference of red blood cell side lobe signals on pulse signal identification of platelets (PLT-O), improve the accuracy and repeatability of pulse signal identification and counting of the platelets (PLT-O), and have the advantages of simple structure, easiness in implementation, low cost and good reliability.
In order to achieve the above object, the present invention provides the following technical solutions:
the utility model provides an optical filter, includes optical filter body and at least one light blocking device be equipped with the logical light hole on the optical filter body, the logical light hole is used for passing through the light beam, every the light blocking device includes a light blocking piece, the light blocking piece can remove in order to shelter from the edge of logical light hole. The light blocking sheet can adopt a common optical filter or a film plating sheet, and can block light.
The optical filter can adjust the position of the light blocking sheet, pertinently filters and blocks edge light beams with high efficiency, and has the advantages of high efficiency, reliability and low cost compared with other small-hole space filters with invariable fixed apertures. Meanwhile, the optical filter can selectively and effectively shield according to the side lobe signal condition, and can also avoid the problems of excessive attenuation of main pulse signals and signal-to-noise ratio reduction caused by excessive ineffective shielding, so that the optical filter can not only effectively eliminate side lobe signal interference, but also maintain the main pulse signal intensity and improve the signal-to-noise ratio.
In one embodiment, each light blocking device further comprises a guiding device, an adjusting device and a fastening device, wherein the adjusting device is connected with the light blocking sheet, the adjusting device can drive the light blocking sheet to move along the guiding device, the position is locked through the fastening device, and the guiding device is used for limiting the movement track of the light blocking sheet. Through the guide device, the adjusting device and the fastening device, the position of the light barrier can be accurately adjusted, so that the elimination of side lobe signal interference can be realized.
In one embodiment, each light blocking device is mounted on the optical filter body, and the integrated design is smaller in size and convenient to process and manufacture. Of course, the light blocking device can be arranged independently without being integrated on the optical filter body, as long as the light blocking sheet is arranged in the light beam propagation direction and can block the edge of the light passing hole.
In one embodiment, the guiding device comprises a guiding pin and a guiding groove, the guiding pin is fixedly connected with the light blocking sheet, and the guiding pin can move up and down along the guiding groove; the adjusting device comprises a screw rod assembly, one end of the screw rod assembly is connected with the optical filter body, and the other end of the screw rod assembly is connected with the light barrier; the fastening device is of a bolt or screw structure.
In one embodiment, the light blocking device comprises a first light blocking device and a second light blocking device, the first light blocking device comprises a first light blocking piece, the second light blocking device comprises a second light blocking piece, and the first light blocking piece and the second light blocking piece are oppositely arranged in a direction perpendicular to the light beam propagation direction. The opposite arrangement means that the edge of the light beam shielded by the first light blocking sheet is opposite to the edge of the light beam shielded by the second light blocking sheet, namely, the included angle between the two is a flat angle. Generally speaking, one of the two light blocking sheets is used for shielding the upper edge of the light passing hole, the other light blocking sheet is used for shielding the lower edge of the light passing hole, and compared with the condition that only one light blocking sheet is arranged, the two light blocking sheets have better side lobe signal eliminating effect and higher efficiency. When the light beam is inclined at a certain angle, the two light blocking sheets are correspondingly inclined at a certain angle, so long as shielding of the light beam edge can be realized, and side lobe signals can be eliminated.
In one embodiment, the first light blocking piece and the second light blocking piece are parallel and spaced along the propagation direction of the light beam. Of course, the two light-blocking sheets may also lie on the same plane, for example integrated in the optical filter.
The first light blocking piece and the second light blocking piece are staggered back and forth along the light beam propagation direction, and the light beam propagation direction has the advantages that part of side lobe signals are blocked by the first light blocking piece, and other side lobe signals are blocked by the second light blocking piece, so that high-efficiency filtering blocking can be realized, light energy loss is low, main pulse signal intensity can be maintained as much as possible, and signal to noise ratio is improved.
The invention also discloses a lighting unit which comprises a light source, a collimation module, a beam shaping module and an optical filter, wherein the light source is used for emitting laser, the collimation module is used for collimating the laser and forming parallel light, the beam shaping module is used for compressing and converging the parallel light, and the optical filter is used for filtering the compressed and converged light beam.
The illumination unit is provided with the optical filter, and the optical filter is used for carrying out filtering treatment on the compressed and converged light beams and inhibiting the influence of edge aberration, stray light and high-order transverse mode mutation of the light source, so that the effect of eliminating side lobe signal interference is achieved.
In one embodiment, the beam shaping module includes a first cylindrical mirror for converging the light beam perpendicular to the junction plane direction and a second cylindrical mirror for converging the light beam parallel to the junction plane direction.
In one embodiment, the first cylindrical mirror and the second cylindrical mirror are disposed orthogonally.
The invention also discloses an optical system for eliminating side lobe signal interference, which comprises a sheath flow cell unit, a signal receiving and processing unit and an illumination unit, wherein the sheath flow cell unit is used for wrapping a cell sample in sheath flow so as to enable the cell sample to pass through an optical detection area one by one, the illumination unit is used for irradiating the cell sample, and the signal receiving and processing unit is used for collecting scattered light and fluorescence information emitted by the cell sample and converting the scattered light and fluorescence information into an electric signal.
The optical system of the invention is provided with the optical filter in the lighting unit and the signal receiving and processing unit, so that the position of the light blocking sheet on the optical filter can be adjusted in a targeted manner through the side lobe signal displayed by the signal receiving and processing unit, thereby eliminating the side lobe signal interference.
In one embodiment, the optical filter of the illumination unit is located between the beam shaping module and the sheath flow cell unit.
Compared with the prior art, the invention reduces the side lobe signal interference by adjusting the divergence angle of the light source, for example, the patent with publication numbers CN100338529C and CN101153868B, and performs the filtering treatment after collimation, shaping and convergence. And the optical filter is arranged behind the beam shaping module, so that the light source has lower installation precision and lower structural cost compared with the arrangement of a diaphragm in the light source or near the luminous point.
In one embodiment, the signal receiving and processing unit includes a front scattered collecting lens, a baffle, a photoelectric sensor and an oscilloscope, wherein the front scattered collecting lens is used for collecting forward scattered light of the cell sample, the baffle is used for blocking direct light, the photoelectric sensor is connected with the front scattered collecting lens, the photoelectric sensor is used for converting an optical signal into an electric signal, the oscilloscope is connected with the photoelectric sensor, and the oscilloscope is used for displaying the electric signal.
The invention also discloses another optical system for eliminating side lobe signal interference, which comprises a light source, a collimation module, a beam shaping module, a sheath flow cell unit and a signal receiving and processing unit, wherein the light source is used for emitting laser, the collimation module is used for collimating the laser and forming parallel light, the beam shaping module is used for compressing and converging the parallel light, the sheath flow cell unit is used for carrying a cell sample in sheath flow so that the cell sample passes through an optical detection area one by one, the signal receiving and processing unit is used for collecting scattered light and fluorescence information emitted by the cell sample and converting the scattered light and fluorescence information into electric signals, and at least one light blocking sheet is arranged between the beam shaping module and the sheath flow cell unit and can move to block the edge of the light beam.
In the embodiment, an optical filter is not required to be arranged, and the edge of the light beam is directly shielded by moving the light blocking sheet, so that sidelobe signals are eliminated, the implementation is more convenient, and the maneuverability is better.
In one embodiment, the light blocking device comprises a first light blocking device and a second light blocking device, the first light blocking device comprises a first light blocking piece, the second light blocking device comprises a second light blocking piece, and the first light blocking piece and the second light blocking piece are oppositely arranged in a direction perpendicular to the light beam propagation direction.
In one embodiment, the first light blocking piece and the second light blocking piece are parallel and spaced along the propagation direction of the light beam.
The invention also discloses a method for eliminating side lobe signal interference, which comprises the following steps:
a light source, a collimation module, a beam shaping module and a sheath flow cell unit are sequentially arranged along the beam propagation direction;
setting a signal receiving and processing unit, which comprises a connecting oscilloscope;
Turning on a light source and an oscilloscope, and adding a cell sample into the sheath flow cell unit;
And according to the pulse signals displayed on the oscilloscope, at least one light blocking piece is arranged between the beam shaping module and the sheath flow cell unit, and the light blocking piece is moved to block the edge of the light beam until the sidelobe signals are eliminated.
The method of the invention adjusts the position of the light blocking sheet according to the pulse signal displayed on the oscilloscope, has simple operation, easy implementation and obvious effect, can effectively eliminate the interference of side lobe signals with lower cost, simultaneously ensures the peak value of the main pulse signal to the maximum extent, improves the signal to noise ratio and ensures the accuracy of pulse signal counting of the platelets (PLT-O).
In one embodiment, a first light blocking piece and a second light blocking piece are arranged between the beam shaping module and the sheath flow cell unit, the first light blocking piece and the second light blocking piece are oppositely arranged in a direction perpendicular to the beam propagation direction, and the first light blocking piece and the second light blocking piece are parallel and are arranged at intervals in the beam propagation direction. The light beam edge is blocked by moving the positions of the first light blocking piece and the second light blocking piece respectively.
In one embodiment, the optical filter is disposed between the beam shaping module and the sheath flow cell unit, such that the light passing hole of the optical filter is coaxial with the optical axis, and the sidelobe signal is eliminated by adjusting the position of the light blocking piece of the optical filter.
In one embodiment, the setting the signal receiving processing unit includes adjusting and fixing the position of the front scatter collection lens, and connecting the photo sensor with the front scatter collection lens, and connecting the oscilloscope with the photo sensor.
Compared with the prior art, the invention has the beneficial effects that:
(1) The optical filter can adjust the position of the light blocking sheet, pertinently carries out high-efficiency filtering shielding on edge light beams, and has the advantages of simpler structure, high efficiency, reliability and low cost compared with other small-hole space filters with invariable fixed apertures.
(2) Because the optical filter can selectively and effectively shield according to the side lobe signal condition, the optical filter only limits light beams in the direction vertical to the junction plane, and compared with the limiting in two directions, the optical filter can avoid the problems of excessive attenuation of main pulse signals and reduction of signal to noise ratio caused by excessive ineffective shielding, so that the optical filter can not only effectively eliminate side lobe signal interference, but also maintain the main pulse signal intensity and improve the signal to noise ratio.
(3) The optical system of the invention is provided with the optical filter in the lighting unit and the signal receiving and processing unit, so that the position of the light blocking sheet on the optical filter can be adjusted in a targeted manner through the side lobe signal displayed by the signal receiving and processing unit, thereby eliminating the side lobe signal interference.
(4) The invention carries out the filtering treatment after collimation, shaping and convergence, and has the advantages of effectively inhibiting the side lobe signal interference caused by the light source internal reasons such as overlarge divergence angle of the light source, high-order transverse mode and the like, and effectively inhibiting the side lobe signal interference caused by the increase of the edge aberration caused by the eccentric tolerance such as the eccentric of the light source, the eccentric of the collimating lens, the eccentric of the shaping lens and the like.
(5) The invention has lower installation accuracy and lower structural cost compared with the light source with diaphragm arranged in or near the luminous point after the light filter is arranged on the beam shaping module.
(6) The method of the invention adjusts the position of the light blocking sheet according to the pulse signal displayed on the oscilloscope, has simple operation, easy implementation and obvious effect, can effectively eliminate the interference of side lobe signals with lower cost, simultaneously ensures the peak value of the main pulse signal to the maximum extent, improves the signal to noise ratio and ensures the accuracy of pulse signal counting of the platelets (PLT-O).
Description of the drawings:
FIG. 1 is a schematic diagram of the detection of blood cells using laser light scattering (PLT-O).
Fig. 2 is a schematic illustration of the spot of the detection zone.
Fig. 3 is a schematic diagram of a generated pulse signal.
Fig. 4 is a schematic diagram of a red blood cell pulse signal and a platelet pulse signal.
The labels in fig. 1-4: the device comprises a 1-light source, a 2-collimation module, a 3-beam shaping module, a 4-sheath flow cell unit, a 5-signal receiving and processing unit, a 6-light spot main peak and a 7-light spot secondary peak.
Fig. 5 is a schematic view of the optical path of the first illumination spot formed by the present invention.
Fig. 6 is a schematic view of the optical path of the second illumination spot formed by the present invention.
Fig. 7 is a schematic diagram of the structure of an optical filter according to the present invention.
Fig. 8 is a schematic structural diagram of an apparatus for eliminating side lobe signal interference according to the present invention.
Fig. 9 is a schematic diagram of the present invention for canceling left side lobe signal interference.
Fig. 10 is a schematic diagram of the present invention for canceling right side lobe signal interference.
Fig. 11 is a schematic diagram of the present invention for canceling double side lobe signal interference.
The labels in fig. 5-11: 1-light source, 2-collimation module, 3-beam shaping module, 31-first cylindrical mirror, 32-second cylindrical mirror, 4-optical filter, 41-first light blocking piece, 42-second light blocking piece, 43-guiding device, 44-optical filter body, 45-adjusting device, 46-fastening device, 47-light passing hole, 5-sheath flow cell unit, 6-front scattered collecting lens, 7-separation blade, 8-photoelectric sensor, 9-first irradiation light spot, 10-second irradiation light spot, 11-main lobe signal, 12-left side lobe signal, 13-right side lobe signal.
Detailed Description
The present invention will be described in further detail with reference to test examples and specific embodiments. It should not be construed that the scope of the above subject matter of the present invention is limited to the following embodiments, and all techniques realized based on the present invention are within the scope of the present invention.
Example 1
As shown in fig. 7, an optical filter includes an optical filter body 44, a light-passing hole 47 is provided on the optical filter body 44, the light-passing hole 47 is used for passing a light beam, and a first light-blocking device and a second light-blocking device are provided on the optical filter body 44.
The first light blocking device comprises a first light blocking sheet 41, a guiding device 43, an adjusting device 45 and a fastening device 46, wherein the first light blocking sheet 41 is positioned at the upper right side (the direction shown in fig. 7) of the optical filter body 44, the first light blocking sheet 41 can block the upper edge of the light passing hole 47, and the first light blocking sheet 41 is installed on the optical filter body 44 through the adjusting device 45 and is locked through the fastening device 46.
The second light blocking device comprises a second light blocking sheet 42, a guiding device 43, an adjusting device 45 and a fastening device 46, wherein the second light blocking sheet 42 is positioned at the left lower part (the direction shown in fig. 7) of the optical filter body 44, the second light blocking sheet 42 can block the lower edge of the light passing hole 47, and the second light blocking sheet 42 is installed on the optical filter body 44 through another adjusting device 45 and is locked through another fastening device 46.
The adjusting device 45 includes a screw assembly, one end of the screw assembly is connected to the optical filter body 44, and the other end of the screw assembly is connected to the first light blocking sheet 41 or the second light blocking sheet 42. The guiding device 43 includes a guiding pin and a guiding groove, the guiding groove is located on the optical filter body 44, the guiding pin is fixedly connected with the first light blocking piece 41 or the second light blocking piece 42, and the guiding pin can move up and down along the guiding groove. The fastening means 46 is a bolt or screw structure.
The invention can independently adjust the positions of the first light blocking piece 41 and the second light blocking piece 42 by arranging the two guide devices 43, the two adjusting devices 45 and the two fastening devices 46. Taking the position adjustment of the first light blocking piece 41 as an example, the screw assembly drives the first light blocking piece 41 to move up and down along the guide groove, and after the first light blocking piece moves to a designated position, the first light blocking piece is locked by a bolt or a screw.
Example 2
This embodiment differs from embodiment 1 in that the first light blocking means and/or the second light blocking means are not provided on the optical filter body 44, the light blocking sheet, the guide means 43, the adjusting means 45 and the fastening means 46 are provided on the light blocking means body, one end of the screw assembly is connected to the light blocking means body, and the other end of the screw assembly is connected to the light blocking sheet.
The first light blocking device and the second light blocking device are arranged back and forth along the light beam propagation direction, and the first light blocking piece 41 and the second light blocking piece 42 are respectively positioned on two different planes, are parallel to each other in the light beam propagation direction and are arranged in a vertically staggered manner.
Example 3
As shown in fig. 8, an illumination unit includes a light source 1, a collimating module 2, a beam shaping module 3, and an optical filter 4 as shown in embodiment 1, the light source 1 is configured to emit laser light, the collimating module 2 is configured to collimate the laser light and form parallel light, the beam shaping module 3 is configured to compress and converge the parallel light, and the optical filter 4 is configured to filter the compressed and converged light beam.
The collimation module 2 adopts a collimator, the beam shaping module 3 comprises a first cylindrical mirror 31 and a second cylindrical mirror 32, the first cylindrical mirror 31 is used for converging a light beam perpendicular to the junction plane direction, the second cylindrical mirror 32 is used for converging a light beam parallel to the junction plane direction, and the first cylindrical mirror 31 and the second cylindrical mirror 32 are orthogonally placed. As shown in fig. 5, the first cylindrical mirror 31 can compress and converge the light beam perpendicular to the junction plane direction at the center of the sheath flow cell unit 5, and irradiates the cell to form a first irradiation light spot 9, where the first irradiation light spot 9 is an elliptical light spot, the long axis direction is perpendicular to the direction of the sample flow, the dimension is between 100um and 200um, the short axis direction is parallel to the direction of the sample flow, and the dimension is between 6um and 20 um. As shown in fig. 6, the second cylindrical lens 32 compresses and converges the light beam parallel to the junction plane at the baffle 7 to form a second irradiation light spot 10, the baffle 7 performs light blocking treatment on the direct light of the light source, the second irradiation light spot 10 is an elliptical light spot, the long axis direction is parallel to the direction of the sample flow, the dimension is between 100um and 200um, the short axis direction is perpendicular to the direction of the sample flow, and the dimension is between 6um and 20 um.
Example 4
As shown in fig. 5 to 6, an optical system capable of eliminating side lobe signal interference includes an illumination unit, a sheath flow cell unit 5 and a signal receiving processing unit as described in embodiment 3. The sheath flow cell unit 5 is located between the illumination unit and the signal receiving and processing unit, the optical filter 4 of the illumination unit is located between the beam shaping module 3 and the sheath flow cell unit 5, the sheath flow cell unit 5 is used for carrying cell samples in sheath flow to enable the cells to pass through an optical detection area one by one, the illumination unit is used for illuminating the cell samples, and the signal receiving and processing unit is used for collecting scattered light and fluorescence information emitted by the cell samples and converting the scattered light and fluorescence information into electric signals.
The signal receiving and processing unit comprises a front scattered collecting lens 6, a baffle 7, a photoelectric sensor 8 and an oscilloscope, wherein the front scattered collecting lens 6 is used for collecting forward scattered light of a cell sample, the baffle 7 is used for blocking direct light, the photoelectric sensor 8 is connected with the front scattered collecting lens 6, the photoelectric sensor 8 is used for converting optical signals into electric signals, the oscilloscope is connected with the photoelectric sensor 8, and the oscilloscope is used for displaying the electric signals.
As shown in fig. 5, after the beam collimation module 2 emitted by the light source 1 is collimated in the direction perpendicular to the junction plane, the first cylindrical mirror 31 can compress and converge the beam perpendicular to the junction plane direction at the center of the sheath flow cell unit 5, irradiate the cell to form a first irradiation light spot 9, the first irradiation light spot 9 is an elliptical light spot, the long axis direction is perpendicular to the direction of sample flow, the dimension is between 100um and 200um, the short axis direction is parallel to the direction of sample flow, the dimension is between 6um and 20um, and the forward scattered light of the cell sample is collected by the forward scattered collection lens 6, and finally converted into an electrical signal by the photoelectric sensor 8 and displayed on an oscilloscope.
As shown in fig. 6, after the beam collimation module 2 emitted by the light source 1 is collimated in the direction parallel to the junction plane, the second cylindrical lens 32 compresses and converges the beam parallel to the junction plane at the baffle 7 to form a second irradiation light spot 10, the baffle 7 performs light blocking treatment on the direct light of the light source, the second irradiation light spot 10 is an elliptical light spot, the long axis direction is parallel to the direction of sample flow, the dimension is between 100um and 200um, the short axis direction is perpendicular to the direction of sample flow, the dimension is between 6um and 20um, and the forward scattered light of the cell sample is collected by the forward scattered collection lens 6 and finally converted into an electric signal by the photoelectric sensor 8 and displayed on the oscilloscope.
Example 5
The utility model provides an optical system that can eliminate sidelobe signal interference, includes light source 1, collimation module 2, beam shaping module 3, sheath flow cell unit 5 and signal reception processing unit, light source 1 is used for transmitting laser, collimation module 2 is used for collimation and the formation parallel light of laser, beam shaping module 3 is used for compressing and converging the parallel light, sheath flow cell unit 5 is used for carrying the cell sample in the sheath flow, makes the cell pass through the optical detection region one by one, still be equipped with at least first light blocking piece 41 and second light blocking piece 42 between beam shaping module 3 and sheath flow cell unit 5, first light blocking piece 41 and second light blocking piece 42 can remove respectively in order to shelter from the light beam edge. The laser emitted by the light source 1 irradiates the sheath flow cell unit 5, and the signal receiving and processing unit is used for collecting scattered light and fluorescence information emitted by the cell sample and converting the scattered light and fluorescence information into an electric signal.
The signal receiving and processing unit comprises a front scattered collecting lens 6, a baffle 7, a photoelectric sensor 8 and an oscilloscope, wherein the front scattered collecting lens 6 is used for collecting forward scattered light of a cell sample, the baffle 7 is used for blocking direct light, the photoelectric sensor 8 is connected with the front scattered collecting lens 6, the photoelectric sensor 8 is used for converting optical signals into electric signals, the oscilloscope is connected with the photoelectric sensor 8, and the oscilloscope is used for displaying the electric signals.
Further, the first light blocking piece 41 and the second light blocking piece 42 are respectively located on two different planes, and the two planes are arranged in parallel in the light beam propagation direction, and the first light blocking piece 41 can block the upper edge of the light beam, and the second light blocking piece 42 can block the lower edge of the light beam.
Example 6
A method for eliminating side lobe signal interference using an optical system as shown in embodiment 4, comprising the steps of:
Step one: the method comprises the steps of arranging an illumination unit and a sheath flow cell unit 5, wherein the illumination unit and the sheath flow cell unit 5 comprise a fixed light source 1, a collimation module 2 and a beam shaping module 3 which are sequentially arranged along the light beam propagation direction, arranging the optical filter 4 between the beam shaping module 3 and the sheath flow cell unit 5, and enabling a light passing hole 47 of the optical filter 4 to be coaxial with an optical axis;
Setting a signal receiving and processing unit, comprising adjusting and fixing the positions of a front scattered collecting lens 6 and a baffle 7, connecting a photoelectric sensor 8 with the front scattered collecting lens 6, and connecting an oscilloscope with the photoelectric sensor 8;
Step two: turning on the light source 1 and the oscilloscope, and adding a cell sample into the sheath flow cell unit 5;
Step three: according to the pulse signals displayed on the oscilloscope, the positions of the first light blocking piece 41 and the second light blocking piece 42 are adjusted until the sidelobe signals are eliminated, and finally the positions of the first light blocking piece 41 and the second light blocking piece 42 are fixed, specifically:
if there is no accompanying small pulse signal, i.e., no side lobe signal, on both sides of the main lobe signal 11, all the light beams can be passed through the light passing holes 47 of the optical filter 4 without performing filtering processing.
If a small accompanying pulse appears on the left side of the main lobe signal 11 and a small accompanying pulse appears on the right side, that is, there is a left side lobe signal 12 and no right side lobe signal 13, as shown in fig. 9, at this time, the screw assembly of the optical filter 4 may be adjusted to move the first light blocking piece 41 downward, filter and block the upper edge light of the light beam, move the first light blocking piece 41 downward while observing the condition of the oscilloscope screen pulse signal until the left side lobe signal 12 on the oscilloscope screen is eliminated, and then fasten the screw locking position.
If a small accompanying pulse appears on the right side of the main lobe signal 11 and a small accompanying pulse appears on the left side, namely, a right side lobe signal 13 exists, and a left side lobe signal 12 exists, as shown in fig. 10, at this time, the screw assembly of the optical filter 4 can be adjusted to enable the second light blocking piece 42 to move upwards, filter and block the lower edge light of the light beam, and move the second light blocking piece 42 upwards while observing the condition of the pulse signal of the oscilloscope screen until the right side lobe signal 13 on the oscilloscope screen is eliminated, and then the screw locking position is fastened.
If the main lobe signal 11 has two side lobe signals, namely, left side lobe signal 12 and right side lobe signal 13, at the same time, called double side lobe signal, as shown in fig. 11, at this time, two screw components of the optical filter 4 can be adjusted simultaneously, so that the first light blocking piece 41 moves downward and the second light blocking piece 42 moves upward, respectively, filtering and shielding the upper and lower edge light beams, adjusting the light blocking piece while observing the condition of the oscilloscope screen pulse signal, until the left side lobe signal 12 and the right side lobe signal 13 on the oscilloscope screen are eliminated, and then fastening the screw locking position.
Example 7
The present embodiment differs from embodiment 6 in that the present embodiment does not require filtering with the optical filter 4, i.e., the optical filter 4 is not required to be provided between the beam shaping module 3 and the sheath flow cell unit 5. In this embodiment, the first light blocking piece 41 and the second light blocking piece 42 are directly disposed between the beam shaping module 3 and the sheath flow cell unit 5, where the first light blocking piece 41 and the second light blocking piece 42 are disposed at intervals parallel to the beam propagation direction and are disposed vertically opposite to each other in the direction perpendicular to the beam propagation direction, and the edges of the light beams are blocked by moving the first light blocking piece 41 and the second light blocking piece 42, so that sidelobe signals are eliminated.
The above embodiments are only for illustrating the present invention and not for limiting the technical solutions described in the present invention, and although the present invention has been described in detail in the present specification with reference to the above embodiments, the present invention is not limited to the above specific embodiments, and thus any modifications or equivalent substitutions are made to the present invention; all technical solutions and modifications thereof that do not depart from the spirit and scope of the invention are intended to be covered by the scope of the appended claims.
Claims (8)
1. An optical system for eliminating side lobe signal interference is characterized by comprising a sheath flow cell unit (5), a signal receiving and processing unit and an illumination unit, wherein the sheath flow cell unit (5) is used for carrying a cell sample in a sheath flow so as to enable the cell sample to pass through an optical detection area one by one, the illumination unit is used for illuminating the cell sample, the illumination unit comprises a light source (1), a collimation module (2), a beam shaping module (3) and an optical filter (4), the light source (1) is used for emitting laser light, the collimation module (2) is used for collimating the laser light and forming parallel light, the beam shaping module (3) is used for compressing and converging the parallel light, the optical filter (4) is used for filtering the compressed and converged light beam, the optical filter comprises a light filter body (44) and at least one light blocking device, the light filter body (44) is provided with a light through hole (47), each light blocking device comprises a light blocking sheet, and the light blocking sheet can be moved to enable the light blocking sheet to emit the light signal to the cell sample and the signal receiving cell (4) to be processed by the edge of the light receiving cell (4).
2. Optical system for eliminating side lobe signal interference according to claim 1, characterized in that each of the light blocking means further comprises guiding means (43), adjusting means (45) and fastening means (46), the adjusting means (45) being connected to the light blocking sheet, the adjusting means (45) being capable of driving the light blocking sheet along the guiding means (43) and locking the position by the fastening means (46).
3. An optical system for eliminating side lobe signal interference according to claim 2, wherein the guide means (43) comprises a guide pin and a guide groove, the guide pin being fixedly connected to the light blocking sheet, the guide pin being movable up and down along the guide groove;
the adjusting device (45) comprises a screw rod assembly, one end of the screw rod assembly is connected with the optical filter body (44), and the other end of the screw rod assembly is connected with the light barrier;
The fastening means (46) is a bolt or screw arrangement.
4. An optical system for eliminating side lobe signal interference according to any one of claims 1-3, wherein the light blocking means comprises a first light blocking means comprising a first light blocking sheet (41) and a second light blocking means comprising a second light blocking sheet (42), the first light blocking sheet (41) and the second light blocking sheet (42) being oppositely arranged in a direction perpendicular to the direction of propagation of the light beam.
5. The optical system for eliminating side lobe signal interference according to claim 4, wherein the first light blocking sheet (41) and the second light blocking sheet (42) are arranged in parallel and at intervals in the light beam propagation direction.
6. The utility model provides an eliminate optical system of sidelobe signal interference, its characterized in that includes light source (1), collimation module (2), beam shaping module (3), sheath flow cell unit (5) and signal reception processing unit, light source (1) is used for transmitting laser, collimation module (2) are used for collimation and form parallel light to the laser, beam shaping module (3) are used for carrying out compression convergence to the parallel light, sheath flow cell unit (5) are used for carrying the cell sample in the sheath flow, make the cell pass through the optical detection region one by one, signal reception processing unit is used for collecting scattered light and fluorescence information that the cell sample sent to convert into the signal reception processing unit still be equipped with at least one light blocking piece between beam shaping module (3) and sheath flow cell unit (5), the light blocking piece can remove in order to shelter from the light beam edge.
7. A method of canceling sidelobe signal interference, comprising the steps of:
a light source (1), a collimation module (2), a beam shaping module (3) and a sheath flow cell unit (5) are sequentially arranged along the light beam propagation direction;
setting a signal receiving and processing unit, which comprises a connecting oscilloscope;
turning on a light source (1) and an oscilloscope, and adding a cell sample into a sheath flow cell unit (5);
According to the pulse signals displayed on the oscilloscope, at least one light blocking piece is arranged between the beam shaping module (3) and the sheath flow cell unit (5), and the light blocking piece is moved to block the edge of the light beam until the sidelobe signals are eliminated.
8. The method of eliminating side lobe signal interference according to claim 7, wherein a first light blocking sheet (41) and a second light blocking sheet (42) are provided between the beam shaping module (3) and the sheath flow cell unit (5), the first light blocking sheet (41) and the second light blocking sheet (42) are oppositely provided in a direction perpendicular to a beam propagation direction, and the first light blocking sheet (41) and the second light blocking sheet (42) are arranged in parallel and at a distance in the beam propagation direction, and a light beam edge is blocked by moving positions of the first light blocking sheet (41) and the second light blocking sheet (42), respectively.
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| CN112687157B (en) * | 2021-01-12 | 2022-10-14 | 上海理工大学 | Teaching experiment instrument of flow cytometry analysis technology |
| CN114674729B (en) * | 2022-03-02 | 2023-11-21 | 迈克医疗电子有限公司 | Pulse identification method, pulse identification device, pulse identification storage medium, pulse identification equipment and blood cell analyzer |
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